LOCUS       DAZKBH010000001       128818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000001 DAZKBH010000000
VERSION     DAZKBH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 128818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 128818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..128818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.001"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..1535)
                     /locus_tag="ACG8OO_000001"
     CDS             complement(12..1535)
                     /locus_tag="ACG8OO_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP9043945.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="ACG8OO_000002"
     CDS             1787..2968
                     /locus_tag="ACG8OO_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9043946.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACG8OO_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACG8OO_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9043947.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="ACG8OO_000004"
     CDS             complement(5053..7764)
                     /locus_tag="ACG8OO_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFP9043948.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="ACG8OO_000005"
     CDS             complement(7774..8475)
                     /locus_tag="ACG8OO_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9043949.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="ACG8OO_000006"
     CDS             8661..9260
                     /locus_tag="ACG8OO_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9043950.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="ACG8OO_000007"
     CDS             9341..10354
                     /locus_tag="ACG8OO_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9043951.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="ACG8OO_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="ACG8OO_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9043952.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="ACG8OO_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="ACG8OO_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9043953.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="ACG8OO_000010"
     CDS             11802..13175
                     /locus_tag="ACG8OO_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9043954.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="ACG8OO_000011"
     CDS             13532..15676
                     /locus_tag="ACG8OO_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9043955.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="ACG8OO_000012"
     CDS             complement(15728..16768)
                     /locus_tag="ACG8OO_000012"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9043956.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="ACG8OO_000013"
     CDS             complement(17045..18847)
                     /locus_tag="ACG8OO_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9043957.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="ACG8OO_000014"
     CDS             complement(18861..19613)
                     /locus_tag="ACG8OO_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9043958.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="ACG8OO_000015"
     CDS             complement(19622..19984)
                     /locus_tag="ACG8OO_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9043959.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="ACG8OO_000016"
     CDS             complement(20160..21032)
                     /locus_tag="ACG8OO_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9043960.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="ACG8OO_000017"
     CDS             complement(21058..21570)
                     /locus_tag="ACG8OO_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFP9043961.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="ACG8OO_000018"
     CDS             complement(21708..22499)
                     /locus_tag="ACG8OO_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFP9043962.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="ACG8OO_000019"
     CDS             complement(22554..23162)
                     /locus_tag="ACG8OO_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFP9043963.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="ACG8OO_000020"
     CDS             complement(23155..23472)
                     /locus_tag="ACG8OO_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9043964.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="ACG8OO_000021"
     CDS             complement(23628..24356)
                     /locus_tag="ACG8OO_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9043965.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="ACG8OO_000022"
     CDS             24515..25396
                     /locus_tag="ACG8OO_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9043966.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="ACG8OO_000023"
     CDS             complement(25633..26676)
                     /locus_tag="ACG8OO_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9043967.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="ACG8OO_000024"
     CDS             complement(26679..27347)
                     /locus_tag="ACG8OO_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9043968.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="ACG8OO_000025"
     CDS             27489..29825
                     /locus_tag="ACG8OO_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9043969.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="ACG8OO_000026"
     CDS             complement(29940..30251)
                     /locus_tag="ACG8OO_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9043970.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="ACG8OO_000027"
     CDS             30432..31907
                     /locus_tag="ACG8OO_000027"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFP9043971.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="ACG8OO_000028"
     CDS             31954..32538
                     /locus_tag="ACG8OO_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFP9043972.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="ACG8OO_000029"
     CDS             32540..32959
                     /locus_tag="ACG8OO_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFP9043973.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="ACG8OO_000030"
     CDS             complement(33240..33524)
                     /locus_tag="ACG8OO_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFP9043974.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="ACG8OO_000031"
     CDS             complement(33876..34586)
                     /locus_tag="ACG8OO_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFP9043975.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="ACG8OO_000032"
     CDS             complement(34583..36055)
                     /locus_tag="ACG8OO_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9043976.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="ACG8OO_000033"
     CDS             36193..36666
                     /locus_tag="ACG8OO_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9043977.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="ACG8OO_000034"
     CDS             complement(36819..37370)
                     /locus_tag="ACG8OO_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9043978.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="ACG8OO_000035"
     CDS             37475..38005
                     /locus_tag="ACG8OO_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFP9043979.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="ACG8OO_000036"
     CDS             complement(38488..38931)
                     /locus_tag="ACG8OO_000036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9043980.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="ACG8OO_000037"
     CDS             complement(38947..40107)
                     /locus_tag="ACG8OO_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9043981.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="ACG8OO_000038"
     CDS             40614..42314
                     /locus_tag="ACG8OO_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9043982.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="ACG8OO_000039"
     CDS             complement(42416..43249)
                     /locus_tag="ACG8OO_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9043983.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="ACG8OO_000040"
     CDS             complement(43553..45718)
                     /locus_tag="ACG8OO_000040"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFP9043984.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="ACG8OO_000041"
     CDS             45978..48272
                     /locus_tag="ACG8OO_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFP9043985.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="ACG8OO_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="ACG8OO_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9043986.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="ACG8OO_000043"
     CDS             48923..49930
                     /locus_tag="ACG8OO_000043"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9043987.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="ACG8OO_000044"
     CDS             49952..50971
                     /locus_tag="ACG8OO_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9043988.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACG8OO_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACG8OO_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9043989.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACG8OO_000046"
     CDS             complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACG8OO_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFP9043990.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACG8OO_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACG8OO_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9043991.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACG8OO_000048"
     CDS             complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACG8OO_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9043992.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="ACG8OO_000049"
     CDS             58397..59698
                     /locus_tag="ACG8OO_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9043993.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="ACG8OO_000050"
     CDS             complement(59767..60366)
                     /locus_tag="ACG8OO_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9043994.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="ACG8OO_000051"
     CDS             60620..61501
                     /locus_tag="ACG8OO_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9043995.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="ACG8OO_000052"
     CDS             61514..63124
                     /locus_tag="ACG8OO_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9043996.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="ACG8OO_000053"
     CDS             complement(63201..63860)
                     /locus_tag="ACG8OO_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9043997.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="ACG8OO_000054"
     CDS             complement(64143..64808)
                     /locus_tag="ACG8OO_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFP9043998.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="ACG8OO_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="ACG8OO_000055"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9043999.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="ACG8OO_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="ACG8OO_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9044000.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="ACG8OO_000057"
     CDS             complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="ACG8OO_000057"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9044001.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="ACG8OO_000058"
     CDS             67122..67442
                     /locus_tag="ACG8OO_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9044002.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68411)
                     /locus_tag="ACG8OO_000059"
     CDS             complement(67554..68411)
                     /locus_tag="ACG8OO_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9044003.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="ACG8OO_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="ACG8OO_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(69819..70328)
                     /locus_tag="ACG8OO_000061"
     CDS             complement(69819..70328)
                     /locus_tag="ACG8OO_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044004.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="ACG8OO_000062"
     CDS             complement(70503..72638)
                     /locus_tag="ACG8OO_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9044005.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="ACG8OO_000063"
     CDS             complement(72721..74376)
                     /locus_tag="ACG8OO_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9044006.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="ACG8OO_000064"
     CDS             complement(74378..74692)
                     /locus_tag="ACG8OO_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9044007.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="ACG8OO_000065"
     CDS             74862..75122
                     /locus_tag="ACG8OO_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFP9044008.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="ACG8OO_000066"
     CDS             75136..75450
                     /locus_tag="ACG8OO_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFP9044009.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="ACG8OO_000067"
     CDS             complement(76180..76734)
                     /locus_tag="ACG8OO_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9044010.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="ACG8OO_000068"
     CDS             complement(76860..78788)
                     /locus_tag="ACG8OO_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFP9044011.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="ACG8OO_000069"
     CDS             complement(79094..79810)
                     /locus_tag="ACG8OO_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9044012.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="ACG8OO_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="ACG8OO_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFP9044013.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="ACG8OO_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="ACG8OO_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9044014.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="ACG8OO_000072"
     CDS             complement(83280..84740)
                     /locus_tag="ACG8OO_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9044015.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="ACG8OO_000073"
     CDS             complement(84730..86025)
                     /locus_tag="ACG8OO_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9044016.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="ACG8OO_000074"
     CDS             complement(86357..87034)
                     /locus_tag="ACG8OO_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFP9044017.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="ACG8OO_000075"
     CDS             complement(87064..88155)
                     /locus_tag="ACG8OO_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFP9044018.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="ACG8OO_000076"
     CDS             complement(88256..88690)
                     /locus_tag="ACG8OO_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9044019.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /locus_tag="ACG8OO_000077"
     CDS             complement(89274..90731)
                     /locus_tag="ACG8OO_000077"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9044020.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="ACG8OO_000078"
     CDS             complement(90728..92623)
                     /locus_tag="ACG8OO_000078"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9044021.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="ACG8OO_000079"
     CDS             complement(92681..94456)
                     /locus_tag="ACG8OO_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP9044022.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="ACG8OO_000080"
     CDS             complement(94506..96002)
                     /locus_tag="ACG8OO_000080"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9044023.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="ACG8OO_000081"
     CDS             complement(96196..97818)
                     /locus_tag="ACG8OO_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9044024.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="ACG8OO_000082"
     CDS             complement(98267..99580)
                     /locus_tag="ACG8OO_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9044025.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="ACG8OO_000083"
     CDS             complement(99825..100049)
                     /locus_tag="ACG8OO_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044026.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100491)
                     /locus_tag="ACG8OO_000084"
     CDS             complement(100069..100491)
                     /locus_tag="ACG8OO_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9044027.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="ACG8OO_000085"
     CDS             complement(100635..101345)
                     /locus_tag="ACG8OO_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFP9044028.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="ACG8OO_000086"
     CDS             complement(101363..101860)
                     /locus_tag="ACG8OO_000086"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9044029.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="ACG8OO_000087"
     CDS             complement(101897..102349)
                     /locus_tag="ACG8OO_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9044030.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="ACG8OO_000088"
     CDS             complement(102484..103089)
                     /locus_tag="ACG8OO_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9044031.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="ACG8OO_000089"
     CDS             complement(103539..104159)
                     /locus_tag="ACG8OO_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9044032.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="ACG8OO_000090"
     CDS             complement(104161..105030)
                     /locus_tag="ACG8OO_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9044033.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="ACG8OO_000091"
     CDS             complement(105053..105460)
                     /locus_tag="ACG8OO_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9044034.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="ACG8OO_000092"
     CDS             complement(105479..106768)
                     /locus_tag="ACG8OO_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9044035.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="ACG8OO_000093"
     CDS             complement(106795..107097)
                     /locus_tag="ACG8OO_000093"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9044036.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="ACG8OO_000094"
     CDS             complement(107122..109215)
                     /locus_tag="ACG8OO_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9044037.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="ACG8OO_000095"
     CDS             complement(109704..110468)
                     /locus_tag="ACG8OO_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFP9044038.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="ACG8OO_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="ACG8OO_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFP9044039.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="ACG8OO_000097"
     CDS             complement(111473..112549)
                     /locus_tag="ACG8OO_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9044040.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="ACG8OO_000098"
     CDS             complement(112573..113166)
                     /locus_tag="ACG8OO_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFP9044041.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="ACG8OO_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="ACG8OO_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFP9044042.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="ACG8OO_000100"
     CDS             complement(114836..115135)
                     /locus_tag="ACG8OO_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFP9044043.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="ACG8OO_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="ACG8OO_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFP9044044.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="ACG8OO_000102"
     CDS             complement(116890..117801)
                     /locus_tag="ACG8OO_000102"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9044045.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="ACG8OO_000103"
     CDS             117921..118778
                     /locus_tag="ACG8OO_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9044046.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="ACG8OO_000104"
     CDS             complement(118965..119327)
                     /locus_tag="ACG8OO_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9044047.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(119516..119698)
                     /locus_tag="ACG8OO_000105"
     CDS             complement(119516..119698)
                     /locus_tag="ACG8OO_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044048.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            119673..121112
                     /locus_tag="ACG8OO_000106"
     CDS             119673..121112
                     /locus_tag="ACG8OO_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9044049.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="ACG8OO_000107"
     CDS             complement(121119..123671)
                     /locus_tag="ACG8OO_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFP9044050.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="ACG8OO_000108"
     CDS             complement(123785..125116)
                     /locus_tag="ACG8OO_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9044051.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="ACG8OO_000109"
     CDS             complement(125082..126560)
                     /locus_tag="ACG8OO_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9044052.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="ACG8OO_000110"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="ACG8OO_000110"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9044053.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        37675 a        26943 c        22534 g        41666 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggaa ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
   128761 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgt
//
LOCUS       DAZKBH010000002        84070 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000002 DAZKBH010000000
VERSION     DAZKBH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.002"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..733
                     /locus_tag="ACG8OO_000111"
     CDS             41..733
                     /locus_tag="ACG8OO_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9044054.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            941..1231
                     /locus_tag="ACG8OO_000112"
     CDS             941..1231
                     /locus_tag="ACG8OO_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044055.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1503..1852)
                     /locus_tag="ACG8OO_000113"
                     /pseudo
     CDS             complement(1503..1852)
                     /locus_tag="ACG8OO_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            1945..3315
                     /locus_tag="ACG8OO_000114"
     CDS             1945..3315
                     /locus_tag="ACG8OO_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFP9044056.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(3555..3638)
                     /locus_tag="ACG8OO_000115"
     tRNA            complement(3555..3638)
                     /locus_tag="ACG8OO_000115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3602..3604),aa:Leu,seq:gag)
     gene            3711..3872
                     /locus_tag="ACG8OO_000116"
     CDS             3711..3872
                     /locus_tag="ACG8OO_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044057.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            4021..5238
                     /locus_tag="ACG8OO_000117"
     CDS             4021..5238
                     /locus_tag="ACG8OO_000117"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9044058.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            5422..6258
                     /locus_tag="ACG8OO_000118"
     CDS             5422..6258
                     /locus_tag="ACG8OO_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9044059.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            6334..7482
                     /gene="nagA"
                     /locus_tag="ACG8OO_000119"
     CDS             6334..7482
                     /gene="nagA"
                     /locus_tag="ACG8OO_000119"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9044060.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            7759..8760
                     /locus_tag="ACG8OO_000120"
     CDS             7759..8760
                     /locus_tag="ACG8OO_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFP9044061.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            8779..9798
                     /locus_tag="ACG8OO_000121"
     CDS             8779..9798
                     /locus_tag="ACG8OO_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFP9044062.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(9900..10616)
                     /locus_tag="ACG8OO_000122"
     CDS             complement(9900..10616)
                     /locus_tag="ACG8OO_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9044063.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(10585..11370)
                     /locus_tag="ACG8OO_000123"
     CDS             complement(10585..11370)
                     /locus_tag="ACG8OO_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9044064.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(11361..12110)
                     /locus_tag="ACG8OO_000124"
     CDS             complement(11361..12110)
                     /locus_tag="ACG8OO_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9044065.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            12408..12932
                     /locus_tag="ACG8OO_000125"
     CDS             12408..12932
                     /locus_tag="ACG8OO_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9044066.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            12948..14447
                     /locus_tag="ACG8OO_000126"
     CDS             12948..14447
                     /locus_tag="ACG8OO_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP9044067.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(14535..15137)
                     /locus_tag="ACG8OO_000127"
     CDS             complement(14535..15137)
                     /locus_tag="ACG8OO_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9044068.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            15241..16197
                     /locus_tag="ACG8OO_000128"
     CDS             15241..16197
                     /locus_tag="ACG8OO_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFP9044069.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(16502..17458)
                     /locus_tag="ACG8OO_000129"
     CDS             complement(16502..17458)
                     /locus_tag="ACG8OO_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9044070.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            17632..18192
                     /locus_tag="ACG8OO_000130"
     CDS             17632..18192
                     /locus_tag="ACG8OO_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044071.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            18185..19009
                     /locus_tag="ACG8OO_000131"
     CDS             18185..19009
                     /locus_tag="ACG8OO_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFP9044072.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            19019..19636
                     /locus_tag="ACG8OO_000132"
     CDS             19019..19636
                     /locus_tag="ACG8OO_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9044073.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            19654..20337
                     /locus_tag="ACG8OO_000133"
     CDS             19654..20337
                     /locus_tag="ACG8OO_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9044074.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            21020..22255
                     /locus_tag="ACG8OO_000134"
                     /pseudo
     CDS             21020..22255
                     /locus_tag="ACG8OO_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            22263..23150
                     /locus_tag="ACG8OO_000135"
     CDS             22263..23150
                     /locus_tag="ACG8OO_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9044075.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            23167..24003
                     /locus_tag="ACG8OO_000136"
     CDS             23167..24003
                     /locus_tag="ACG8OO_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9044076.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            24006..24629
                     /locus_tag="ACG8OO_000137"
     CDS             24006..24629
                     /locus_tag="ACG8OO_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044077.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            24626..25918
                     /locus_tag="ACG8OO_000138"
     CDS             24626..25918
                     /locus_tag="ACG8OO_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9044078.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      26277..26374
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            26490..27071
                     /locus_tag="ACG8OO_000139"
     CDS             26490..27071
                     /locus_tag="ACG8OO_000139"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9044079.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            27074..28381
                     /locus_tag="ACG8OO_000140"
     CDS             27074..28381
                     /locus_tag="ACG8OO_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFP9044080.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            28674..29162
                     /gene="purE"
                     /locus_tag="ACG8OO_000141"
     CDS             28674..29162
                     /gene="purE"
                     /locus_tag="ACG8OO_000141"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9044081.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            29155..30279
                     /gene="purK"
                     /locus_tag="ACG8OO_000142"
     CDS             29155..30279
                     /gene="purK"
                     /locus_tag="ACG8OO_000142"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9044082.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            30292..31587
                     /gene="purB"
                     /locus_tag="ACG8OO_000143"
     CDS             30292..31587
                     /gene="purB"
                     /locus_tag="ACG8OO_000143"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9044083.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(31767..32144)
                     /locus_tag="ACG8OO_000144"
     CDS             complement(31767..32144)
                     /locus_tag="ACG8OO_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044084.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            32400..33128
                     /gene="purC"
                     /locus_tag="ACG8OO_000145"
     CDS             32400..33128
                     /gene="purC"
                     /locus_tag="ACG8OO_000145"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9044085.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            33128..33382
                     /gene="purS"
                     /locus_tag="ACG8OO_000146"
     CDS             33128..33382
                     /gene="purS"
                     /locus_tag="ACG8OO_000146"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFP9044086.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            33384..34055
                     /gene="purQ"
                     /locus_tag="ACG8OO_000147"
     CDS             33384..34055
                     /gene="purQ"
                     /locus_tag="ACG8OO_000147"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFP9044087.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            34056..36278
                     /gene="purL"
                     /locus_tag="ACG8OO_000148"
     CDS             34056..36278
                     /gene="purL"
                     /locus_tag="ACG8OO_000148"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFP9044088.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            36263..37702
                     /gene="purF"
                     /locus_tag="ACG8OO_000149"
     CDS             36263..37702
                     /gene="purF"
                     /locus_tag="ACG8OO_000149"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9044089.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            37725..38777
                     /gene="purM"
                     /locus_tag="ACG8OO_000150"
     CDS             37725..38777
                     /gene="purM"
                     /locus_tag="ACG8OO_000150"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9044090.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            38774..39352
                     /gene="purN"
                     /locus_tag="ACG8OO_000151"
     CDS             38774..39352
                     /gene="purN"
                     /locus_tag="ACG8OO_000151"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9044091.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            39349..40884
                     /gene="purH"
                     /locus_tag="ACG8OO_000152"
     CDS             39349..40884
                     /gene="purH"
                     /locus_tag="ACG8OO_000152"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9044092.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            40900..42174
                     /gene="purD"
                     /locus_tag="ACG8OO_000153"
     CDS             40900..42174
                     /gene="purD"
                     /locus_tag="ACG8OO_000153"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9044093.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            42296..43210
                     /locus_tag="ACG8OO_000154"
     CDS             42296..43210
                     /locus_tag="ACG8OO_000154"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP9044094.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(43343..44908)
                     /locus_tag="ACG8OO_000155"
     CDS             complement(43343..44908)
                     /locus_tag="ACG8OO_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9044095.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(44901..46352)
                     /locus_tag="ACG8OO_000156"
     CDS             complement(44901..46352)
                     /locus_tag="ACG8OO_000156"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9044096.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(46366..47382)
                     /locus_tag="ACG8OO_000157"
     CDS             complement(46366..47382)
                     /locus_tag="ACG8OO_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9044097.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            47602..48690
                     /gene="chvE"
                     /locus_tag="ACG8OO_000158"
     CDS             47602..48690
                     /gene="chvE"
                     /locus_tag="ACG8OO_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9044098.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            48744..50270
                     /gene="mmsA"
                     /locus_tag="ACG8OO_000159"
     CDS             48744..50270
                     /gene="mmsA"
                     /locus_tag="ACG8OO_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9044099.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            50272..51477
                     /gene="mmsB"
                     /locus_tag="ACG8OO_000160"
     CDS             50272..51477
                     /gene="mmsB"
                     /locus_tag="ACG8OO_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFP9044100.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            51734..52711
                     /gene="guaC"
                     /locus_tag="ACG8OO_000161"
     CDS             51734..52711
                     /gene="guaC"
                     /locus_tag="ACG8OO_000161"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9044101.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            52975..54399
                     /locus_tag="ACG8OO_000162"
     CDS             52975..54399
                     /locus_tag="ACG8OO_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9044102.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(54441..54698)
                     /locus_tag="ACG8OO_000163"
     CDS             complement(54441..54698)
                     /locus_tag="ACG8OO_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044103.1"
                     /translation="MPIRILTALVNKITIRKAFYFSSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            54860..55969
                     /gene="pdhA"
                     /locus_tag="ACG8OO_000164"
     CDS             54860..55969
                     /gene="pdhA"
                     /locus_tag="ACG8OO_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFP9044104.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            55972..56949
                     /locus_tag="ACG8OO_000165"
     CDS             55972..56949
                     /locus_tag="ACG8OO_000165"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFP9044105.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            56974..58617
                     /locus_tag="ACG8OO_000166"
     CDS             56974..58617
                     /locus_tag="ACG8OO_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP9044106.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            58625..60031
                     /gene="lpdA"
                     /locus_tag="ACG8OO_000167"
     CDS             58625..60031
                     /gene="lpdA"
                     /locus_tag="ACG8OO_000167"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9044107.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            60193..60468
                     /locus_tag="ACG8OO_000168"
     CDS             60193..60468
                     /locus_tag="ACG8OO_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFP9044108.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            60474..61241
                     /locus_tag="ACG8OO_000169"
     CDS             60474..61241
                     /locus_tag="ACG8OO_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFP9044109.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            61409..63238
                     /gene="typA"
                     /locus_tag="ACG8OO_000170"
     CDS             61409..63238
                     /gene="typA"
                     /locus_tag="ACG8OO_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFP9044110.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            63499..65271
                     /gene="recQ"
                     /locus_tag="ACG8OO_000171"
     CDS             63499..65271
                     /gene="recQ"
                     /locus_tag="ACG8OO_000171"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFP9044111.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            65385..65687
                     /locus_tag="ACG8OO_000172"
     CDS             65385..65687
                     /locus_tag="ACG8OO_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFP9044112.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            65690..66853
                     /locus_tag="ACG8OO_000173"
     CDS             65690..66853
                     /locus_tag="ACG8OO_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9044113.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            66879..70307
                     /locus_tag="ACG8OO_000174"
     CDS             66879..70307
                     /locus_tag="ACG8OO_000174"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFP9044114.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            70328..71452
                     /locus_tag="ACG8OO_000175"
     CDS             70328..71452
                     /locus_tag="ACG8OO_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFP9044115.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            71433..71879
                     /locus_tag="ACG8OO_000176"
     CDS             71433..71879
                     /locus_tag="ACG8OO_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9044116.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            71863..72201
                     /locus_tag="ACG8OO_000177"
     CDS             71863..72201
                     /locus_tag="ACG8OO_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFP9044117.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            72320..72880
                     /gene="rsmD"
                     /locus_tag="ACG8OO_000178"
     CDS             72320..72880
                     /gene="rsmD"
                     /locus_tag="ACG8OO_000178"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFP9044118.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            72877..73368
                     /gene="coaD"
                     /locus_tag="ACG8OO_000179"
     CDS             72877..73368
                     /gene="coaD"
                     /locus_tag="ACG8OO_000179"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9044119.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            73358..74401
                     /locus_tag="ACG8OO_000180"
     CDS             73358..74401
                     /locus_tag="ACG8OO_000180"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFP9044120.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            74560..75288
                     /locus_tag="ACG8OO_000181"
     CDS             74560..75288
                     /locus_tag="ACG8OO_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9044121.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            75404..75649
                     /locus_tag="ACG8OO_000182"
     CDS             75404..75649
                     /locus_tag="ACG8OO_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9044122.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            75646..75864
                     /locus_tag="ACG8OO_000183"
     CDS             75646..75864
                     /locus_tag="ACG8OO_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9044123.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            76000..76539
                     /locus_tag="ACG8OO_000184"
     CDS             76000..76539
                     /locus_tag="ACG8OO_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFP9044124.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            76532..78805
                     /locus_tag="ACG8OO_000185"
     CDS             76532..78805
                     /locus_tag="ACG8OO_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFP9044125.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            78843..79889
                     /gene="holA"
                     /locus_tag="ACG8OO_000186"
     CDS             78843..79889
                     /gene="holA"
                     /locus_tag="ACG8OO_000186"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9044126.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(79978..80232)
                     /gene="rpsT"
                     /locus_tag="ACG8OO_000187"
     CDS             complement(79978..80232)
                     /gene="rpsT"
                     /locus_tag="ACG8OO_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9044127.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(80632..80901)
                     /locus_tag="ACG8OO_000188"
     CDS             complement(80632..80901)
                     /locus_tag="ACG8OO_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044128.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(81163..81291)
                     /locus_tag="ACG8OO_000189"
     CDS             complement(81163..81291)
                     /locus_tag="ACG8OO_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044129.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(81371..81826)
                     /locus_tag="ACG8OO_000190"
     CDS             complement(81371..81826)
                     /locus_tag="ACG8OO_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9044130.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(81964..83892)
                     /locus_tag="ACG8OO_000191"
     CDS             complement(81964..83892)
                     /locus_tag="ACG8OO_000191"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9044131.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        27392 a        14376 c        18109 g        24193 t
ORIGIN      
        1 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
       61 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      121 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      181 gcttgatcat acattgacag ataaagagca agcgcttaca gatcttaccg ctcgactagc
      241 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      301 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      361 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      421 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      481 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      541 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      601 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      661 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      721 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
      781 tatactgctg cctctttttt ttagtacatg tccttcatct tcgcaggaag tgccaatatg
      841 attttaagaa aggtaataga caaaaagtca aaaagataaa acagagtttc ttctttacaa
      901 agggaatgta tgttcgtata atggtattga ggtgaaggtt atggagaaaa aatatggaac
      961 agtagataag atccgtactt tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa
     1021 tggggtcaat tgtttgattg cttcccatag tttgaacttc ctagctgaag tagacgaagg
     1081 gatgaagtta gtcgttacag gttattataa taatagaaag caatttgttg tgagatccta
     1141 tcatgtgtta gggaaaccca aaattgtggt agaatatgag aaaagtctat acccaagaaa
     1201 aaaggtccga caagatgaaa gaataaacta gaaaacaagc ggctgagaca aaaatgtcct
     1261 agccgcttgt tttggaaaga tggatgcaga cgatgggatt tgaacccaca cgagcatacg
     1321 ctcacaagaa cctcaatcta tatataacta catgtattta agtatttgtg atgaatcact
     1381 aaactttcca aactcatttg agggatattg cttaaagatt gaacagatca attcgaagcc
     1441 ctattagtat tcgctcaatg gggggatcag aggccataaa aagagactag tcattactag
     1501 tctcaggtag tggaattgta ggattttaaa cgttcctgga gagtaccggt atgtaactcg
     1561 aagaggtgat tatcatagtc gtaaaagtaa attgaagaag cctcgccttt cacacgagaa
     1621 cgtcctggtt cgacagttaa acctaacatt tggatttttg agacaaacga atctatatct
     1681 tgttcgtcaa ttttaaaagc aatatgattg tatgtctttg gcagtttatt cgacgaattc
     1741 tgcattactg caatccatag gtccttaact aagaaaaatt tttctggata taatgaaaat
     1801 ttttttgttg actatcgtag atttcttttg catcaaataa ggtatttaac aattaagtat
     1861 ttaaaagata acttttaagt tatctttaag taatgcttaa agctatgtta tactctatta
     1921 ggaaatataa gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa
     1981 gaactgatct tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg
     2041 ccgactatga gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg
     2101 ggtgcttcga tagaacatta taagacatgg agttcaagaa ttattataga actattctta
     2161 atgttctttt ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga
     2221 attgtagtat tactttgcaa gtatgttttt gagaaaataa atgttaaaaa tttattattg
     2281 gtttgctcta tatactgttt aataccattg acagtaatgg gggaaaccgg ttggagagca
     2341 accaccttga actatcagtg gccagtagca tttagtttac taacttttta tccgtttttt
     2401 caactcttaa gaggagaaga aataaataga aaaatatatt gggtaagtat tcctttattg
     2461 atatttttaa ctaatcaaga acaagtcaac gcctgttttt ttgttctaac aagtatagtt
     2521 agtttatatt tgattgtcaa tggacgatat aattataagt tatcagtatt ttcaataata
     2581 agcttagcag aattaatttt ctctttgaca actccaggga atgcattacg tgctgcacat
     2641 gaaattaata aatggtttcc tgaatataaa aatttcaact ttcttaataa gttagattta
     2701 ggtatttcat catttggaaa gccgtttttt ttagacatga atatcttatt tttattgcta
     2761 tttttcttaa ttttccttct aacatatagg aaatgtcaaa actattatgt gcgtatattg
     2821 actgcattac cgtttttctt aaacctgatt atttattttg gtaatacaat gggtcaaagc
     2881 tttacttacg tcaatggaaa taaaagggca atgatttgga gtagtagtaa cctgaataat
     2941 ctctttacag agttaggaac gaaactgtca cttttttatc caggtacttg gatagctact
     3001 ttagtagtct tggcattatt actatgttta attgttggaa tttatttaag ttttgacaat
     3061 aagaaaactt ctattttttt agttattttg atgataatgg gattttgttc aaggttaatt
     3121 atgggatttt ctcccacagt atgggcttcc ggaatgcgga cgtactatat actttatgtt
     3181 gttatcgcca tcttagtact aatggcagtt aaagagctaa tgaaaagcat gagtgtacaa
     3241 aaaaatgaat ttatgcaatt tggattaacc gtgcttggta tttgtacctt tataataacg
     3301 gtgataaaca gataatatac aaaaaatccc tcgctttagt attaggtgag ggattttact
     3361 cgtttttcga ttaattgttt taacacttgc aagtttctac gagtagcttg tttccgacct
     3421 tcatatactc acctcaggct gtttacatga ttgtacgttc tttttgtaaa ggatacagtg
     3481 atatgtatat aatggcataa agaaacgttc aaacaaaaaa actcgtgaag caaaattaac
     3541 ttcacgagtt gtagtgcaga tgatgggact cgaacccaca cgagctaacg ctcacatgac
     3601 cctcaatcat gcttgtctgc caattccaac acatctgcgt aataataaca cactaatgat
     3661 agcgaggttt tacatagttg tcaagctcaa aaatgggagg acttattaag atggagaaat
     3721 atgacggaga attttcagga ctgggaatga tcttaggtgt attgattgga cttgcattcg
     3781 gacgtttttt gcttggtttt atgctaggaa tcatttgtgg aattgcaatg gattgggcag
     3841 cgaatctatg gaatgactat catgacaatt aagttctcaa cgcttgtttg tgttttttta
     3901 atacttacgt ttttcctaaa taagtggcaa aattttctag gatggagtat gatattgaaa
     3961 tagtaaataa caaaaaattt ggaaagttct aactttttga aatgagggat tggctaagtg
     4021 atgagagaat ttgaaaaatc caacaaatta gaaggtgtca gttacgatgt tcgcggtccc
     4081 gttctagaag aagcggagcg gatgcaagaa gagggcatta gtattttaaa attgaacacg
     4141 ggaaatcccg caccttttgg atttgaagca ccaaacgaag tcattcgtga tttgatcatg
     4201 aatgcccgtg actcagaagg ctactcagat tccaagggga tcttttctgc tagaaaagca
     4261 attgagcaat attgccaact gaaacatttt ccgaatgtga cgatcaatga tatctacacc
     4321 ggcaacggag tgagcgaatt gatcacgatg tgtatgcagg ggttgctgga caatggagat
     4381 gaagtactgg ttccaatgcc ggactatccg ttgtggacgg cttctattgc attagctgga
     4441 ggcacacctg ttcattatat ttgcgatgaa gaggcagaat ggtatcctga tattgatgat
     4501 atcaaatcaa aaataacgag tcggacaaaa gctattgtta tcattaatcc taataatcct
     4561 actggggcac tttatccaaa agaattatta gaagaaatcg ttgaagtagc tcgacaaaat
     4621 aacctgatta tttattccga cgaaatctat gatcgcttag tgatggatgg tctagaacat
     4681 attccgattg ctacattagc gcctgatttg ttcgttgtta cgctgaacgg tctatccaaa
     4741 tctcatcgtg tagcaggatt tcgagtaggc tggatggtcc taagtggaga taagtcacac
     4801 gtcaaaggct atatagaagg gctgaatatg ttgtcctcca tgcgtttgtg ttccaatgta
     4861 ttgtctcagc aaattatcca aaccgcgcta ggcggttatc aaagtgtcga tgagctgctc
     4921 ctgccaggcg gaaggatcta tgaacaaaga gaatatatct ataatgcaat caatgatatt
     4981 cctggtcttt cagccgtaaa accaaaagct gctttttata tattcccaaa aattgataca
     5041 aagcgattca atattttgga tgatgaaaaa ttcgttttgg actttttaca tgaacatcat
     5101 attctacttg tacatggtgg tggatttaat tggaaccagc cagatcattt tcgaatcgtt
     5161 tatcttccta aaatggatga tttgaaacaa acagctaaga aaatgagaga attcctagca
     5221 acctatcgcc aaaaatgatt ttaaaaacca aatttttcag taggagggcc acagacaaac
     5281 acactgaggc tctctttcta gtggagtttt cttaaataac taaagtatat tatttagttg
     5341 caagtctttt cttgaagttt gcgacttcct aaagtaaaat acgaatagaa tgcgaatacg
     5401 catattttgg ggaggatact tatggaaacg ttaatcgagt cctttggaac gatcattaaa
     5461 gagattcgaa aagaacaaaa aatgacgcaa aaaatgcttt cgcatgatat ttgttcacaa
     5521 agtgtattaa gtcggatcga aaataatgag gaactgccga atgtattggt gatgatgcag
     5581 atttgtcaac ggctaggggt aacgattgat catgtgatga atctctctcg aaaaactact
     5641 cggacaaacg ataaattatt tgaactgttg gatagttgtt tccaaaaacg tgattatcgg
     5701 aagttagaac aagtactaaa atcttctgat atttctgaaa atctgtatca agcaactgac
     5761 tttcaacgct attattatta tctcggtgtt tgcgaattca cgttgcgtca aaatatctct
     5821 cagtcccttt attatttgaa agaagcatta agctactcta gccaaaaaga aagaatccat
     5881 gtttctgaca cagaaataca actgattagt tgtatcggaa aaatatatgg tgtggcagga
     5941 aaaacggctg aagcacgcta ctatttgagt cgaagcatcc aactgctgca tcaatcagct
     6001 gatgaacgat caaaaagtga attttcgcaa attttttata attatggaaa tttcttattc
     6061 catcaaaatg aaatcgacga agcgttagaa caggtgaatc aaggaatcta ttgggctcaa
     6121 gagaagaata gctactatta cctcaatgat ttatttgtgc ttaaaagctt aatatacaag
     6181 cgtaaagaag aaccagaaag agcagcattc tatgaaggct tagcacaagc tgtgaaacaa
     6241 atttcaaaaa atttgtaaat ggtgtagacc attcttgact tcttctctaa ataagagtac
     6301 tttagaggta gaaaaacaaa gggagagaat agaatgagaa cttttatcta tgctgacaaa
     6361 ttctttttag cgtcaggtgt caaacatgca ggctatcttg aaattaccga tggacttttt
     6421 ggtgaataca ccaaagaact acctgaggga gaagtcaaca tcattgacca aagcggcaaa
     6481 tggattgctc ctggtttggt agatacacat attcacggct atatgaatca tgatgtaatg
     6541 gataatgatg cagaaggcat caaagtgatg tctgaaggtc tgttatcttg tggagtgact
     6601 tcatttttac caacaacatt gacgtcaagc aaagaacgtt taaaagatgt agctgagaca
     6661 atcggcaaaa tgtatcagga agcaccagga gctaaaatca aaggaatcta ttttgaaggt
     6721 cctttcttca cagaagaaca caaaggggca caaaacccaa gttatttcgg ggatcctgat
     6781 ttggatactt tccatgaatg gcaagaagct tcaggtggtt taatcaaaaa aattgcttta
     6841 gcaccggaac gtcaaggtgt agaagagttt gtcaaacaag tgactgaaga aggagtagtt
     6901 gtttctctag gacacagtaa tgctacacta gaagaagcgc aaaaagctgt tgaagcaggc
     6961 gcaagtgtat ttgtccatgc tttcaatggt atgcgtggat tgaatcatcg cgaaccaggt
     7021 atggtgggtg cgttattatc tttagaacat gtcttttctg aattgatttg tgatggccat
     7081 catgtacatc caaatgctgc gggaatccta atggaaaaag caggacatga tcatgtcgca
     7141 ttaattacag actgtatgat ggccggtggg atgcctgatg gaaattacaa tttaggcgaa
     7201 tttccagtag tagtaaaaga aggaacagca cgtctagaat ctggcaactt agctggaagt
     7261 atattgaaat tgaaagaagc aattaaaaat gttgtagact ggggattagc aacaccagaa
     7321 caagcagtca tgatggctag tctgattcca gcaatcagct gtaaaatcga tgatacatgt
     7381 ggaaaaatcg ctcaaggacg cgatgcagac tttatcgtat tgaaccctga tatgacattg
     7441 gaagcaactt atctagatgg cgtggaacgt ttccacgcat aatataattt aagttaacct
     7501 taaaactaaa cttcttaagt ttggttttaa ggtttttttg tgttctagta aaaatcactt
     7561 tacataagtt tcaaaagttt ctttaatttc ctattgttca ataagtaaca atgtaaagat
     7621 aaaattaaaa atgttaatac gtattagcaa aataaaagct ttttttattg tgaataaatg
     7681 aacataatta ggaggagaaa gatgaactta tttgacattt ttcgatcgac tttgacagat
     7741 atgtcgattg tcagtgcaat cacttcaaca gtttttatta tattattagg gtttgtttgt
     7801 agaaaaaagg ggattttttc tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca
     7861 gtggctttgc cagcactggc atttcgagct ttcatgcaag atatcaatac agaattactg
     7921 atgcaaggaa tcaatgtctt gatttgggga ttagttattt atattattct tattctagtt
     7981 tcaaaacctt ttttcttaag ctataaaggg gataagcaaa caacgttgcg tgtcctgacg
     8041 atctttggtt ctacgacctt tttcggaacg cctatcgtca gtgcgattta tggtcctgtc
     8101 ggcgtgatgt attcctcaat tttcaatatt ggctaccgaa tctttttgta ttcttatgga
     8161 tacataaaaa tgagtggctt gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat
     8221 ccaatcatta tcgctacttt tgccggcctt ttcatttggc tgttccaagg gtatctgcca
     8281 caagtgtcag ttacaggtgc agaaggagag ataagccaag tagcgttttt gcgaattgac
     8341 cagactgcac cgtggctatt tcagccgatg gattatttag ctggtttaag ttcaccattg
     8401 gcgtggctag caatcggcgc aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa
     8461 aaaacttcat ggtattacag tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg
     8521 attctagcag ttttgacggc aacgaatatt ctaccagtca attttgaagc actggcaaca
     8581 atcgttatca tgatggctac accagctgca acagtggcag cagcctatgc tatcagtttt
     8641 gatagggagg cacttttagc ctccaatgca tccttactgt caactatttt tgctgtggta
     8701 ctgacacctg tatggatcgt tgtattacaa atgattcatg gcataggttt attttcataa
     8761 gaaatggagg aagaaagtat gagaatcgca tgttacggtg ttcgtccgaa cgagatagat
     8821 ttttttgaaa aattgaatat ttaccattat gatctcagtc tttatgaaga actgttgaca
     8881 catgacaata tcgtgtcagc aaaggaccat gatgctgtat tgttgagggg aaattgtata
     8941 gcagatgaga ccaatcttgc aaaaatgcag gaatatggga ttcgttatgt ttttacacgg
     9001 actgtgggag tcaatcatat cgatctacaa gcggcagctg attttggtat gacagtagcc
     9061 cgtgtaccat cgtattcgcc aaatgcgata gcagagttat ctttgacttt tgccatgatg
     9121 cttttgagaa atactgcgta tacgacgatc cgaacatctt tcaaagattt tcgagtggat
     9181 gagcaaatgt ttagtcgtga aatcagaaat tgcacagtag ggatcatagg aacaggcagg
     9241 atagggttga cggaagctgt tttgtttaag ggactaggag ccaaagtgat cggctacgat
     9301 attcatcaga cagaagctgc taaaaaagta ttgacgttcc actcgcttga agagttatta
     9361 gccaaaagcg atattgtcag tattcatgta cctcatatcc caggtgaaaa cgatcaaatg
     9421 atcaatgaag gatttttagc tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt
     9481 ggagagcttc aagataatga agctatttta agagctttga aaagcaatca tttagctggt
     9541 tttgcaaccg atgtttttgc aaatgaaaca gaggtttttt tccgatcctt caagccatgg
     9601 gagacgattc cagaccctgc tatccagcaa ttagtagaaa tgtaccctag agttcttgta
     9661 accccgcatg taggttcaaa tacagatgag gcattatcaa atatgatttc gacaagtttc
     9721 gagaactttc gtgaaataat agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa
     9781 gcccgtcaat taagataaga aaacatggat attcgtcaat aaaatgttta aaaatagtcc
     9841 aagtaaacag ttgttacttc tgtatacttg gactattttg tatgggataa ttcgatgact
     9901 caacagtaat ctgtgagctt tttccgccca ttctttgtca attcataaca ggttagcggc
     9961 tggccgattt ccaaatattt gattctttcg ttcaatatat cttgttctac taaaaaattc
    10021 aaatatttct tgacggatat acgtgacagt gagctttttt gagccagttc ttttgttgaa
    10081 aaaatagtat cttcttttag aataaatgca cttatttttt ttaaagtcag ttttgacaga
    10141 cctttaggca aatcgatgaa tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact
    10201 tgatcgatca atttctgatc ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga
    10261 tacttttcga tcgccagctg aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag
    10321 gcaccgtagc ggatcacatt ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc
    10381 actgggatat catttccttg ctgtctaagc caagataaaa aatggatacc actttcgcca
    10441 ggcatgtata catcaagcag cagcagatct gcgttttttt gtctgaaata tttttttcct
    10501 tcttctacat ttctgaaact ttcgattacg ttcatattag ggatgctttc cacaaaagta
    10561 cgattcaatt ctgcaaccat tggatcatct tcgattatca ggacatttgt catcattttg
    10621 atcttccttt tttggtaatt ctagatagac gattgtaccg ccatttgttt gtttggaaaa
    10681 ttctaaaaat ccaccgtggc gtttgacgat tgaatggatg agattcaatc caaacccacg
    10741 attgattccc ttcgtcgaaa aacctagcgt aaatattttt tctgagattt cttctgatat
    10801 cccgacaccg ccatcgcaga cggtcacgat gaatacttca gcttctgtgt catagtttaa
    10861 atgaatccgg acctgtccgt ttctttcatg tttttgtagc acggcttctt ttgcattatc
    10921 aagtaagttc cctaacactt gcaaaagtga atggatggag tcatccatct ccattttagg
    10981 gataaaagaa tcgctttcta acagcatttc aacagattgt tctttggctt cgtttatttt
    11041 gcctaataaa aaaccggcaa ttgcaggtac tttgatcaga cttgagactt ggccaatctc
    11101 ttttttataa ttttgattta gctgctggat aaaatgggtg acttcttcat acttcttcaa
    11161 atcaatcaaa ccagaaatga catgaagttt attcatgaat tcatgggttt gagctctcat
    11221 ggagtttata tattgtttcg tccccccaag ctcctccatt aatttcatta cttctgatcg
    11281 atttcgtaaa gttgcgactg ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct
    11341 agaaatagtt tctatcccat ttaaatacat ggattgagtc cgaacggtcg tttgatgctc
    11401 aatcgtctct ttaaacaaaa cgtcatatag attatcactg atttttttcc tacttcgaaa
    11461 tccaatagct gctctgtttg gtagaaaatg ttttttgcag gtgtattgat caagaggata
    11521 gttttatcag aagagatggc taagattcct tcatctactt cattttcgat aatcgttttt
    11581 tcacttaact gttctgctat ttcttgtggt tcaagtccaa gcagtacttt tttcattttt
    11641 tgggcaagaa aaacggcgcc tatgatgcct acgctaagtc caataatcaa tgaaaaaaca
    11701 atcatttttt gtccatcttt gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa
    11761 actgcaccaa taatctcttg atcataataa atagggacga aatagcggaa gccgaaccct
    11821 aacattcctt ctttttcaga gaaaaattct tgtcctccaa tcactttttt cacatcttca
    11881 tgacttgaga aaggaagacc gatgtaactt gggtctgcgt gagaatatcg aatgccttca
    11941 gggtccatga caacgataaa atctaaattc gatgcaatca tgatttcatt tgttattcct
    12001 tgaatcgcat gtgtcgggtc accagtctgc agcttttttt ggatgagttt atcattggca
    12061 acgattttag caatgctaga gattttatct gacacactag atcggttcat cgaaagaata
    12121 aaattcctga tcaatatggc agaaacaatc aaagaaacga atgtggagaa acaaacgaca
    12181 aggatgatcg tgttctggag ctttaatggt tttttcttaa aaaaaggaaa cataggctac
    12241 ctcgaaagaa tttattgtga aaattttaac atgaaaacaa aaatttaaca aattgtcaac
    12301 gataaacaca accttcctat aaaaaacacc actgagtcgg taaaagaagc tatacagaat
    12361 tttcaaacat tggtatgata gacgtagaaa tggaaggagg cgtgtatatg atagggggaa
    12421 tcgcacttga gacgatttat tggtatacat tgctgatttg tgcaggattg gcagtcctat
    12481 tgattatttt tggagatgta ttcgattttg atggcccgct tgatccgatg cttattatcc
    12541 cttggttaac tttcacaagc ttatttggtt ttctaggtga gcgtttaaca gatcaatcat
    12601 ctttattttt atttattgct agtggaggca tatcgatagt gttggtattt ttgcttaatt
    12661 tctatatatt gatgccgatg aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc
    12721 tggaaggaca agtagctgtt gtgatcacac cgatccctgt acgaggtatg ggagaaattc
    12781 agttgaaaag tgttaccggt tccattagtc gtccagcttg tttttacagc ccccaagaac
    12841 agacagctga gcggggaacg aaagtattga tcattgagat caaggaaaga atctgttatg
    12901 tcatgcctta tgaaagcatg ataaaagctt aaaggagcgt gtgttaaatg gaaataccag
    12961 ttgtttttat cgcgatcgca atcgcagctt tgattttact gatgttgctg atcgtctttg
    13021 tagcgaagta ccagacagca aaaccggatg aagcgttgat catcagtgga agttatttag
    13081 gaagcaaaaa tgtgcacatt gatgaaagca ataacaaaat caaaattgtg cgtggaggtg
    13141 gcgcattcgt cttgccagtt tttcaacgtt cgaatcggat cagtctatta tcgagtaaat
    13201 tggacgtctc cacccctgaa gtgtataccg aacaaggcgt accggtgatg tgtgatggta
    13261 cttcgatcat caaaatcggt tcatctgtag aagagattgc tacagcagca gaacagtttt
    13321 taggcaagac aagagaagaa ctggaaaatg aagcaagaga agtattagaa ggacacttgc
    13381 gctcgattct tggttctatg actgtagaag aaatctatca aaatcgcgat aagttcagtc
    13441 aaagcgtaca agaagtcgct tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt
    13501 tcactatcaa agaggtcaga gacaaaaacg gttatcttga ctctttaggg aaaccaagaa
    13561 tcgcccaagt caaaagagat gcagatatag cagaagcgga agctctaaaa gaaacgcgta
    13621 tcaaaaaagc tcaagcagaa aaagaatcac aagcagctga attacagcga caaacagaga
    13681 tcgcagaatc attaaaagaa aaagaactga aattagcaac ctacaaacaa gaacaagatg
    13741 ttgctaaagc aaaagcagac caagcctaca atttagaaag tgcccgagca caacaacaag
    13801 taatcgaaca ggaaatgcag attaaaatcg tagaacgcca aaaacaaatc gaactggaag
    13861 aaaaagaaat cacacgtcgt gaaaagcaat atgattcgga agtgaagaaa aaagcagatg
    13921 ccgatcggta cgctaaagaa caagaagcac aagctcaaaa agtcaaagaa gtgactgaag
    13981 ccgaagcaga acgattccgt gtggaagcac tggcagaagc ggaagccaat aagacacgtt
    14041 tagctggaca agcagaagcc gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa
    14101 aacaaaagat cgccaatgcc ttcaaggaat atggcgaagc agctgtcttg agtatggtta
    14161 tcgatatgct accgcaattg atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa
    14221 tctctgttgt cgatacagga agcagttcag gagaaaccag tggagcgaac cgagtgacaa
    14281 attatgccac aaatttgctt tcttctactc aagaaacact aaaagcgaca acaggacttg
    14341 acttgaagga aatcgtagag aatttgtcga cacgtgggac tactagccaa gtaaatatgt
    14401 atgaagcgcc aaaagaggca gaacaaaact cgatgaagga agaataaagt agtacacttc
    14461 accgttcaat caaaaatgga ggaagacttt ttaaagttct tctctccatt tttttatttt
    14521 attgcaaaag aaaattagtc ctcgctattt ttcttttgtt tttggatctt agcttctatc
    14581 tcttgtaaat ttgttcttag caatcgttct ttcttttgga gttcgaccaa atcttctgat
    14641 agttcttcct tcttttctgt ttcaggagga cgaagaaaga aattgatacc gattgcaatg
    14701 aatgtcccta taaaagtatc tagtacacgt tggatagcat acaaagcgga ttcgccttga
    14761 gggatgctca acgcaatcag caacaacgta gctattgcgg aaatgatacc ggaattattg
    14821 ttaataccat ctgacacgac gatcacaata ataactagaa taggaagcaa gatcaattct
    14881 accaaaaaat cattgtgaaa atagcttttg acaaaaaaat aaatgatagc taatgtacca
    14941 cctaatgtat tgccgatgat cctagacttt ccaaaagaaa cggttgtagt caagtcctga
    15001 cgcaaagaaa aaacagcaga taatgcagca atcatcggga ttcctcgttg agtcaaatga
    15061 aaaaacaaga tacataaaaa aacagctaag gcacttttga tcgtacgcat acctagtcga
    15121 aaacggccaa attgcataga gattctcctt tttggtcttt ttactatcat acctaaactt
    15181 ttgtctgata gctagttttt cattattcaa gtataattaa aataaaaaag ggggaagtaa
    15241 atgcgtatag gaatgcgaac agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca
    15301 gcgggtagct tatcgctgtt gtattggcca gctgcaggga tcattgcatt attaagtgta
    15361 ggaaatacca agcgctctac attgcgtacg ggcatgaatc gaatcgcagc ctttatatta
    15421 gctacggcaa cagcactgtt cagttttttt gctgttgggt atactatttt tggcttcagt
    15481 ctttttttat tcctgttcat cccgcttgcc tctcgcttca aattaaccga agggatagta
    15541 gtaaattcgg ttttggtcac tcattattta gtggaggaaa acatgtcttg gcagctgatc
    15601 ggcaatgaag cgggattaat ggcgattgga ttagtatttg ctttgcttgc aaatgtttac
    15661 atgccagatc gtacgaagca gctgaaggaa aaccaaatac agatcgaaaa agaatttcga
    15721 aatattttga gacaaatggc agaatttctt ttatcagaaa ataaagaaga tgtgcagatc
    15781 aaatgcgaac atttactgac attcattcgc gaaagccaag aagatgccag agaacatcaa
    15841 gaaaactact ggctcagaca gccgctatac tacgaaactt acttctctat gagaagagca
    15901 caggccaatg tgattaaaga catgctggaa aatctagaga gaatccagca accagcttat
    15961 tatggaaaac atatctatgg tttattgatt tatactgcag aaacattttc agagtcaaat
    16021 gatggcagac aaattttaac acgtattgaa gaagtttatg tattgtatcg gcaaatgcct
    16081 ctgccgacta gtcgccctga attcgaggat cgagcagaat tgttccagtt tttgcagtct
    16141 tttaaaagtt ttattgaaat caaggcagaa ttctcccaac aaaaaatcca aaaatagagg
    16201 ttgtgaattc agctaatatt caggtcaaaa agctgatttc acaacctctt ctttaaacgg
    16261 tgttctcctg tcaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
    16321 ttttgaaatt gctccttgtg actcggagct gaacggtagg ctcacaacct ctttcttaaa
    16381 cggtgttcaa aagctttcct ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc
    16441 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca accttttaaa
    16501 attacatatt tttccataga acttgcattt gatccaagat ttcgatgaca tcttttgtca
    16561 agaagagaga gcgattgggc aagtcacctt caatcatgtg taccatattc tttatttcgt
    16621 aattcattgc ttcagatgta ttcccgcttt cgatgaattc ctttgtacca tctgtaaata
    16681 agatctccgc acgatcagca cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg
    16741 caacgatacc gacttttggc atttttgcct gaaaagtcaa gctgacagca gcaagttcat
    16801 tttctttgtt acgcaagata gtgacggatt gttcgtccac gcctgtttca aaaggaacca
    16861 ttgtagaggc aactacttca ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat
    16921 acgtaccgat atccaacaga gctcctccag ctaattctgg attgaagaac cggtttttag
    16981 ggtctggttc tttgtaactg ccgaaaggtg cttggatcat ttttaaagcc cccagttttc
    17041 ctgtatccat tattgagcgt aactgctgat aaagtggcat gttgaagatt gtcatcgctt
    17101 ctgctaagat caaattcttt tcttctgcta agcgcatagc gtcagccagt tcttttttat
    17161 tcatcgtgat ggctttttca catagaacgt gtttgcctgc ttccaatgct gccaagatat
    17221 ggtcgatatg ctgtctattt ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta
    17281 acatttcttg atagcttccg taagcttttg gaatattgta acggtgagcg aagttttccg
    17341 ctttttctga agttctggaa gcaactgctg ttagttcgct cgtctcttga ttgaagcttt
    17401 cggcaaactc atgtgcgatg gtaccaagac caacgattcc ccattgatat tttttcatct
    17461 gaaaacgctc cttcatagtt ggattgagtt tcttgtctgt ctattattat aacaaaaatc
    17521 acgaagaata aagaggagag gacttgaaac attaaaaaaa atgaaagcag cgatttaaga
    17581 ggtgcttttt gatcaaaact taagtagaat agttaaaagg aggggataaa cgtgcatcaa
    17641 gtatatattg atcttattat gagtgtatta ttagaacagt ttgaaactga aaaaaatttt
    17701 ttagatgaat acttgctgat cagtccagaa caatggcaaa aatggaaaga tggcgagaaa
    17761 aatctgccat ctgaagcgat gcagaaagta aaaaatcttt tcactgatta tgaatggatg
    17821 ctaacgcaaa agattttgcg tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt
    17881 gcagagtaca agcaaatcaa aacacgcatc gcgcaaaaat ggcttagcag caaagcagca
    17941 aaagtcgagc tgatcccttt gaaaaataat cagcaggctg gtagatacct tgacttgaaa
    18001 gtaagtattc aatatgatga atggggctat gatgatattt tgaattttcg tctgccagca
    18061 gatgttcaaa aacagatcga aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat
    18121 ctggaagaaa cttacgttca tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt
    18181 tcaagtgaat aaatttttag gatttggagc agttgtgatt atgatcgcag caatgggcta
    18241 tgttattttt agcgatgatg attcgcatgt tcaagccggc aatcaagcag tcgaacgaac
    18301 aagcaccaaa acaactgcta gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa
    18361 ggacaaagaa acaaaagatc gtacgtccaa acaattgcca gatgttaaaa cagacgattg
    18421 gaagctagtt ttggttggtc cagatcacaa attagaaaaa gaaattgatg agtctactca
    18481 attggcatca ttaagtaacg gatacttgat cgacaaacgc attgctccag aatatgaagc
    18541 atttgccaag gctgctgaag cagctggatt tccattagtg atggtctcag cttaccggtc
    18601 tgtatcatcc caacaacaag ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg
    18661 tgtatcagaa gaggaggcga caaagattac taaagaaacg attacggagc caggatacag
    18721 cgaacaccac acaggtctgg cagtcgatgt cgtcgatcaa aactggtaca acagttatcc
    18781 ttctactgta ttggatgcaa gttacggaga tcagcctgga gctaaatgga tcgcagataa
    18841 tgctgcgcag tatggattta tcgttcgtta tccaaaagga cacgaagata taactaagat
    18901 cacatatgaa ccttggcact tacgttatgt cggtaaagaa aacgctgagt atatcaccaa
    18961 acaccaattg acgctagaag aatatctgaa tgaattaaat gaaaagtagg aattgatcat
    19021 ggcgaaaaaa aagtggaaaa agcaaaagcg caagaatatc cgttggccag cagtggttgc
    19081 cggtgtagca gtgatactct tagcctttgt tttttccttg aagaatctgt cttctccttt
    19141 tactgataat cagcaagaaa atgaagcgca atcccatcag caatttattg aacggcttgt
    19201 tccgcatgcc caagaactac aagatggtta tggagtgctt cctagtatca ttttaggaca
    19261 agctatatta gaatcaaatt ggggaaaaag taccttggct agcaaataca ataatctttt
    19321 cgggatcaag gcttatgggg atcagaagaa agtaagcctg gaaacaaagg aatttgttaa
    19381 cgaagaatgg ataactatcc aaggagactt caaagtgtat gattcatggg aacagtccat
    19441 ggatgaccat acacaactgt tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa
    19501 agtgattacg gccaccaact atcaggaagc cgcacaagca ttgcaagatg caggttatgc
    19561 gacagacccc ggttatgcgc aaaaaatcat ccaggtcatc gagacgtatc aattgaatca
    19621 gtatgaccgg agatagcaaa ggagaagtgg ctgatggcag aacaatttac gccagaaatc
    19681 atgacggaat tgagaaaaga tatcgtaaga gtaccggaaa tcattttaga agcaagcggg
    19741 atcaagatat ttggaaaatt gatccgttcg atcatcttta ctacggatat tgcgatcata
    19801 cgtaatacaa atgcgaatgc agtaattgct gtgtatcctt ttacacctca tccagcgatt
    19861 actaaaagca ttattgaggc agctgacatt ccagttttct ccggtgtagg tggtggtttg
    19921 acacaaggtg ctcgttcttc ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg
    19981 gtcgttttga acggtcctac gcctttagag acagtaaaag aagtttgtcg agtagttgat
    20041 attccagtta tcagtacggt aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct
    20101 ggtatttcga ttatcaatat cagtgcaggt aaagaaactg cagaagcagt acgccatttt
    20161 cgtaaaaaat atcctgaact tcctatcatt gcgacaggtg gtccaacgga agaaagtatt
    20221 gctgagacca ttcaagcagg agcaaatgcc atcacatata cgccaccaag tagcggagag
    20281 ctttttagtc gtaaaatgga aaaatatcgg gaaactgaga aaaagaatca tgaataacag
    20341 gtgagaatca aaagacgagg aagagcagat agtggctctt cctcgtcttt tgtactccag
    20401 tactatttgt tcagaattca aaaaaaagag aaattttttt gtttttttac ctgtaatgta
    20461 cataagaaga gttaaaacct tttgttatta ttagcattct aacaaaaaaa tgtcttgatt
    20521 tattgaagcg ctttctatag aatgaaaaac gaataaaaac taaaaaaggc acccgacaat
    20581 ttagtagcag ctaaattgtc ggggcttgaa aagtcagcgg gaactgacca atcaagttgc
    20641 catagtcaat gcttgtgcat cgacgtgtat attgtaccta atttcataca gaattactag
    20701 ttatttttct gttttttagg gaagagcgca cacgaagaag catgcttatt tagctatgtc
    20761 aaggatattg ggcagttgtc tgatatcctt ttttctttgc gtaattatcc tcccatcatt
    20821 gaagcaagaa gtgtttttag cgatgaaaga agaatcagca ctatctaatt tttcttgaaa
    20881 gtattgagtt gttcatccta ggcagaacga taaggcaagt cttggttttt attccccttg
    20941 aaaaagatcg tttaataaat atgttctgta taaaaataca gtatattgaa aaataacaat
    21001 ggaagtagag gtgcagagaa tgaagaaaag aatgagaatc atcttagctt taagtgttat
    21061 gttattcttc ttgacggctt gtgggcaaga agatgacaaa aaaatcacgc tgaacatggc
    21121 ttggtggggt tcgcaagtcc gacacgatgc aacagtaaaa gtaatcgagc tttatgaaga
    21181 aaaaaatccc catgtgacga tcgattatga attttatgat tatgaaggat attacacaaa
    21241 attaggtaca ctagcagcat cagatgatat ctgggacatt ttccaactga acaatcaatt
    21301 tcctcaatac atcaatcaga ttgaaccatt agatgaatat gtggagcaag ggataattga
    21361 tatttctaat gcagatgaat cctatttagc aacgacgact tacgacgggc aattagttgg
    21421 actgtctaac ggggtgaatt cgtttgcgat tgcttacaat aaaaatatat ttgaacaatt
    21481 aaatatccct gaaccagctt caaattggac atgggatgaa tttgaacaaa tcagctatca
    21541 aatcactgaa gaattaggaa tctatgctat ttcaagattc gaagatttta tagctggctg
    21601 tattattcaa ataccacaag ttgagaaagg attgaatttt tataatcggg aagatctatc
    21661 taacagcttg ggatttgaaa atccagacta tctaacagat tttttccagc tgcgtaaaaa
    21721 tctggtagac aaaggggccc atccagaacc aggtggtgga gctgcaagta cgacggatct
    21781 gtcttttcag gcggttaggg atagagaagc ggcaatggtg tttctatcaa gtaaccagtt
    21841 gacagaaata ttgtcagggg ctcctgaaga tatggagatt cagttgatta atccacctcg
    21901 cagaaaagca gatggagaat ctggtatccc tttacgttct tcgcaaatgc tgagtatggc
    21961 aaaaagctca gcccataaag aagaagctgc taagtttatt gatttctttc aaaacagtga
    22021 agaagccaat gaaattttaa aaggagaacg tggtgtacca atcatgtcga atattcgaga
    22081 acagctcgct cgtgcagatg atgcaacttt gcaagcgaca tttgattatt tagaaatgat
    22141 ttccgatatg gacaacggcg aagtgaatgt ctttgatagt gctttgaatc cggaaattca
    22201 agaccaatac aatcttttag ttgaaaaagt catttatgaa gaagtaacag cagaaggaag
    22261 gcatgaaaaa agaaaaacta aaacggttct tggatcaaga caatgtggtc ggttacgtat
    22321 ttgcttctcc attcatccta ggatttctat tgataacagt gataccgatg atcttgtcac
    22381 tttattattc gttcaccgat tatcgtttgg gaaatccagt ctcatggatc ggcttggata
    22441 attatataaa gatgctgagt gatgcccgtt ttttgaactc catccgagtc acttttctct
    22501 atgtatttgc atctgtacct gtgaaactga tctttggtct attgattgct tatcttttaa
    22561 cgaggaatat caaaggaacg actttctttc gttctttgta ttatgtgcct agtttgatcg
    22621 gtggcagtat ttctatcgct ctagtttggc gggagttgtt ttctaaaaac gggttagtga
    22681 atattttcct ggaacgatta ggcgcacctg cgctttcctg gtttagcgat caaaaactgg
    22741 tcatgatccc tcttgttttg atgtctgctt ggcaattcgg ttcggcaatg atcatctttg
    22801 ctgcaggtat caaagagatc ccaggctcgt attatgaagc ggcagagatc gatggagcga
    22861 acaaatggca gacgttttac aagatcacgt tacctcttct ttcgccgatc attttgtata
    22921 acttggttat gcagacaatc gcggcgttta tgacttttac gcaggcattt atcattaccc
    22981 aaggtggtcc gaatgatgcc acgaactttt atgctcttta tgtgtataac caagcttttt
    23041 cttttaattc tatggggtat gctagtgcta tttcgtggat catgctgttg attatgggag
    23101 ctgtcacctt tctgattttc aaatcatcaa aaaaatgggt cgttgtgtga atgagaggag
    23161 gaagaaatga ataatacagt aacgaaatgg tttcaatacg gttttatttt gctattaggt
    23221 ctagtcatga tttatccggt cttatggatg atcgcaggtt cttttaaaac ggatcaagag
    23281 attatgagtg gttcgctgaa tttgattcca gagacttttc aatgggaaaa ttacgcaaaa
    23341 ggatgggcgg ggtttgctgg aatttcattt tttactttct ttaggaattc gtttgtcatc
    23401 tctgcagttt cgacgatagg gacagttctg agctcgactt gtattgctta cgcattatcg
    23461 agaatcgatt tcaaaggaaa aagattctgg ttcgtcgtca tgttagtaac gatgatgatt
    23521 cctgcacaag tgattttgat tccgcagttt attatttaca atcggctgaa tttggttggg
    23581 acgtatgtgc cgctgatttt accacatttt tttggacaag catttttcat ttaccagatc
    23641 atgcaattca tggtcagcat ccccaaagag ttagatgaat cagcgattat tgacggatgc
    23701 agcaagtata gtgtctttac caaaattatt tttcctttgc tgaaaccttc gatcatcacg
    23761 acgatcatca tccagtttta ttggaaatgg gacgacttca tgggaccatt gatttattta
    23821 aacaagccga gatcttacac ggtatcgatt gccatcaaat tatttgcaga cgctggatct
    23881 agtacgactt acgcgagtat gtttgcgatg tctacgttgt cgctgctacc ggtttttctg
    23941 attttcttat tcttcaataa atatcttgtc caggggatca gtacaagcgg tctgaagggc
    24001 tgaatatgaa aaagccaata gaagagatgt ttctttacaa gctcaactac tatgtctgtc
    24061 ggattttttt aaccagtctt atcagtaatg ctcttttcgc tttagttcta ggatgtttgt
    24121 tcactttagg attagtgctg tctgctcgtg ttgtctatct gatttatttt ttgatgatgt
    24181 atccttctta ttatctatct gtccattact tggcagaaaa aaaggtatgt tggaggaaag
    24241 ggaaaatcaa acggatgtgt ttcatcatta cgttgtttca gctgtttgtc attatttttg
    24301 cagttgattg gtatttatta gtgggaaaga ttggagcttg gtatctgatt ccttttctat
    24361 tgtgtctgtt gctttttgta tcccatagtt tttttagtac gctaattgat atctgcaaag
    24421 aagataaagc agatagtctt gtttatttga tcaaaaggaa tatctggctg ttgtcaaaaa
    24481 acgtcaaggg gtttctgtgg tatttatgcc ttttgtatgg actgttctgg tgtatacagc
    24541 ctgatttttt actcactgca ttactgtatc cagggctgcc atttgtcaca gtattttgct
    24601 taaaagaaaa ggagaatgac tttaaatgaa gaaaaaatat gtacaagtcg gacttggtgg
    24661 gagagcgcgt tttttttatg aggcaattgc ctctacgtat catcaaactt gtgaactagt
    24721 cggtttttgt gatgtatctc aaacaaggat ggattatgca aatcggatac ttgtgaacaa
    24781 gtatggacag aaaaaagtaa agacatgtat gccatcacaa tttgagcaac tactggatga
    24841 cgtacggccg gctgaagtca ttattacgtc agtagatcga acgcatcatg attatatcat
    24901 tcgtgcaatg aaaaaaggat atgatgttat ttgcgaaaaa cctctgacta tcgatgagga
    24961 aaaagctcaa gaaataataa cggtccaaaa acagacggga agaagcctgc gagtcacttt
    25021 taattatcgt tatgcaccgt atcacacgaa gataaaggaa ctgatagaaa gtgatgtgat
    25081 tggtgatgtg ttttctatcc acttcgaatg gctgttaaat acgcaacacg gggcagatta
    25141 tttcagaaga tggcatcgaa ataagcacaa tagcggcggg cttcttgttc ataaatctac
    25201 ccatcatttt gatctagtga atttttggtt gaacagccgt ccccaaaagg tatttgcatt
    25261 cggtggatta aacttttatg ggatgcataa tgcggagaaa cgcggagtag aaatatttta
    25321 cagtcgatgc tatggaagcg aactagctaa agctgatcca tttgctatag acctgtcagc
    25381 caatgaggaa ttgcgagaaa tgtatttaga agcagaaaaa gacagtggat atattcgtga
    25441 ccgcagtgta ttttccgatg atatctctat tgaagataca ctaggcgtaa cggtcaaata
    25501 tgaaactggt gcgattttga cgtattcgct aaacgcgtat atgccgtggg aaggattcaa
    25561 tgtttctttc aatggcagca aaggacggat cgaaatgaaa gtgatcgaaa agtcttacat
    25621 caatgcaggt ggagataaaa aggatgaagg agcagccgtt cttcagtcca tccatgtcta
    25681 tccgatgttc ggtgagccat atgaggtaat cgtcgaacaa gaagcgggcg gacatggcgg
    25741 aggagatccg cgtttattag atgatctatt tggtgaagaa aaagaagaag atccgtggaa
    25801 tcgggcagca actcacgtag atggtatttt gtcgattttg acaggaatcg cagcaaatca
    25861 ttctattgct tcaggaaaag cagtagtgat tgacgagctt gtcagcttaa aagaataaga
    25921 atagatttac ggaaattact tcccgtagtt tataaaatct tgccttattg ccgataggct
    25981 gtcttttgaa cactatttat tcggaaaaag gggctatgac atgctttttg tcatgccttc
    26041 tttttatgtg ggctgctttt ttagatagga gaaaataaaa ataactattt aaacccgaac
    26101 aaaaaggaat aatgcattaa aaatcattag attttacaaa aaaataagta aaccacgttg
    26161 tcagtttcag aaagtagtgt tagactgttt tcaatatttg aaacaacatt ttttattcaa
    26221 atatggcaaa ctgccttcac gtgcaaaaca gttgcaagaa aatgaattta cactattatt
    26281 agttttacat acctacttat atatcgtcat aatatggatg acagtttcta gccagtaccg
    26341 taaatgctgg actataagta aaagattggc tatttcaccc ttcttttctt atgataaagt
    26401 ggagggtgtt tttctttaac agaaacatag taggtttttg cacaaaagcg gttatttcgg
    26461 tattaataaa tgataaagga gcaaataaag tgaaagaatt agttgaacgt attcaaaaag
    26521 acggacgtat attaggcgag ggcgtattaa aagtcgacag cttcgtcact caccaagtag
    26581 accctgtatt gatggaacaa atgggaaaac ggtttgcaga agtatttaaa gaacaaggaa
    26641 tcacgaaagt tgtgaccatt gaagcttctg ggatcgcacc agctttattt gctgcaagag
    26701 aattagatgt tccaatgatc ttcgctagaa aagcaaaaag tttgacgatg gatgaagaat
    26761 tattgacagc ttctgtttat tcttttacga aacaagtgac aagtacgatt tctatttctc
    26821 gtaaattttt atcttcagaa gacaaagtat tgattatcga cgatttcttg gcaaacggac
    26881 aagcggcaaa aggattgatc gaattatgcc agcaagctgg agcgcaagta gaaggaatcg
    26941 ggatcgtcat cgaaaagtct ttccaagatg gacgtcagtt acttgaagaa atgggattga
    27001 gagtcgtttc tcttgcgcgt attgcttcct tgacgaatgg acaagttgag tttcttgagg
    27061 aggacgcata atcgtggaaa aagaaacaca aaatggaaaa gctgcggttt tagggctgca
    27121 gcatctgcta gcgatgtatg caggagcagt agcggtgcct cttttgatcg gtacaggact
    27181 aggctttaat caagaacaga tgacttattt gatttcaatt gatatcttta tgtgtggatt
    27241 agcgacatta ttgcaattga cagttaaccg atttttcgga attggcttgc ctgtcgtttt
    27301 aggatgtgcg atccaagcag tagctccact gatcttgatc ggaaccaatg aaggagtagg
    27361 agctatctac ggatctatta tcgcttctgg gatctttgtt gtgttagttt ctggtgtctt
    27421 ttctaaaatt aaaaaactat tccctcctat agtaacagga acggttatca ccgttattgg
    27481 tttgactttg attcctgtag caatcgagaa aatgggtgga ggtaatgcaa cagcagctga
    27541 ttttggtgac aaaacaaatc tgctacttgc atttgtgacg attctcctaa ttgtaggcgt
    27601 gcaaatgctg gcaaaagggt tcattcgttc aatcgcggta ttgattggtt tagtcggagg
    27661 aagcatctta gctgctttca tgggaatgat tgatctgagc gcgattggag atgcgccgtt
    27721 gttccatgtt ccacaaccct tttattttgg taaaccaact ttcgacgtat ggtcgatttt
    27781 actaatgatc atcatttcca tggtcagcat ggtagaatca acaggtgtat attttgcact
    27841 tggtgacatt actgggaaaa atgttggaga agaagaattg aaaaagggat atcgtgcaga
    27901 aggacttgcc gttatcttag gcggtatttt caatacattc ccttatactg gcttttctca
    27961 aaatgtcgga ttggttcaac tttcgggaat caaaacaaga aaacccatct atttctctgc
    28021 attcttcttg attgttttag gattattccc taaaatcggt gcgttagcac aaatcatccc
    28081 tgagccggta cttggtggag gaatgttagt catgttcggg atggtggcag tccaaggaat
    28141 ccgtatgctg ttggaagttg atttcagcaa tgataaaaat ctattgattg ttgctgtttc
    28201 tgttggattt gggctgggtt tcaatgttac gccgacatta ttcagtcaaa tgccgcaaac
    28261 attgcaaatg ttcactggaa atggcatcgt aatgagtagc attacggcta tcatattgaa
    28321 tttgatcttt aatggattga aaaaagatga agaaatattg gaagaagcct tcaagaagta
    28381 atagagcaaa agaaaggttg tgaaatcagc gttttgacct gattgttaga gaaacaaacg
    28441 gaaaaatagt tcctcgtatt ttgccgtttg tttgagctaa taccgagggt cagctgattg
    28501 aacaccgttt atccataaaa aggaatgtga cctaactttt gtcacattcc tttttttcat
    28561 ctttaataaa cacgaacaat aaaatgtttt tctttgttaa cattcgaaat ttctttgaca
    28621 tcaaaaaaag tgattgttaa aatatgaatg tagaaaaact gggaggaaaa acaatgacag
    28681 caaaaatctc tgtgattatg gggagtactt cagattggga aacaatgaaa gaagcatgtc
    28741 aaatattgga tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc
    28801 cagatttgat gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg
    28861 caggagcagg gggagccgcg catctgccgg gtatggtcgc atcaaaaaca actttgccgg
    28921 ttatcggtgt accggtccaa tcacgaacat taaatggttt ggattcgctt ctttcgattg
    28981 tccaaatgcc tggaggagta cctgtagcta ctacagcaat cggaaaagcg ggagcaatta
    29041 atgctggact acttgcgata cagatgcttt ccatgtatga tttagaattg gaggctaaat
    29101 tggctgaaag acgttcaatg ttggctaaaa ctgttataga aagtagtgat caacttggtt
    29161 aaacctttgt tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg
    29221 atggcgatta gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat
    29281 tgtccagccg cacaagttgc agattggcac attgaaagtg tctatgatga cacctttgcg
    29341 ctgaaagagc ttgccagaag aacagatgtc attacctatg aattcgaaaa cgtcagtgta
    29401 gaggcgttaa atgcgatttt acccatgtca tttattcctc aagggacaga cttgcttgcg
    29461 attacgcaag accgcctgtt agaaaaatct tttttggaaa cgaataacat cgtgattgct
    29521 ccttatgcaa cgatcgtcag tccgacggat atccaagatg cgattgacgg gatcggctat
    29581 ccttgtgttt tgaaaaccac tcgaggcggc tatgatggca aggggcagta tgttttgaaa
    29641 agtcgggcgg atctagctcc tgccatggac cttttaagag aaggaacctg tgttttagaa
    29701 gcatggatcc cgtttgaaaa agagatttcg atcatggtgg caggaaatgg tcaaggagat
    29761 ttcacgactt ttccggtcgt ggaaaacatc caccacaaca atattcttca tgaaacgatt
    29821 gctccagccg cgattgatca ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt
    29881 gcagaagcag tgagcctttc aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc
    29941 gggctatatg tgaatgaatt agctcctcgt cctcacaatt ctggacatta ttctattgaa
    30001 gcttgttcta tgagtcagtt cgatgcccat attcgaggta tttgtggttg gccattagga
    30061 gaaaaagtac gtttgataag caatgcagta atggtcaatg ttttgggaga acaactgctg
    30121 gaaagctatc gcttgattga gaataagcca gactggcaat tccatttcta tggaaaaaaa
    30181 caagcaaaaa aaggtcgcaa gatgggacat atcactatct tgacagaaga catccagcaa
    30241 acgctggaag aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa catgttagaa
    30301 cgttatacac gccctgaaat ggggaatatc tggacggaag agaacaaata ccgtgcgtgg
    30361 ctcgaagtcg agattttagc agatgaagct tgggcagaac ttggtgaaat cccgcaagaa
    30421 gatgtgaaaa aaattcgtga gcatgcatca tttgatatcg atcggatctt ggaaatcgaa
    30481 aaattaacac gccatgatgt cgttgcgttt acacgagcgg tttctgaaac attgggagaa
    30541 gaaagaaaat gggtccatta tggtctaaca agtacggatg tcgtagatac tgcttatggc
    30601 tatctcatca agcaagcaaa tgatatttta cgggaagact tgaaacgttt tacggaaatc
    30661 attggcgaaa aagcaaaaga acacaaatat acagtgatga tgggtcggac acacggtgtc
    30721 catgctgaac caactacttt tggattgaaa cttgccacat ggtattcaga aatgaaacgg
    30781 aacatcgaac gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca
    30841 gtgggaacgt tcgcaaatat cccgccattt attgaagagt atgtatgtaa acaattgggg
    30901 atccgtccac aagaaatctc tactcaagtt ctccctcggg atctacatgc tgaatatttc
    30961 tcagcgatgg cgcttattgc tacaagtatt gaacgttttg caacagagat acgcggattg
    31021 caaaaatccg aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa aggttcttct
    31081 gctatgccgc acaaaaggaa tccgatcggt tcagaaaata tggctggtct agccagagtg
    31141 gtgcgcggac acatggtcac tgcctatgaa aatgtcacat tatggcatga aagagatatt
    31201 tctcattctt cagcagaacg aatcatcttg cctgatacca cgattttatt gaattacatg
    31261 ttaaatcgtt ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg
    31321 aatatgtctg caacatttgg tttgatttat agccagcgcg ttatgctgaa attgatcgat
    31381 aagggaatga caagagaaga agcttatgat cttgttcagc caaaaactgc gtacgcttgg
    31441 gacaaccaag tgcagttccg cccgctgtta gaagaagatc caaccatcac atcggtatta
    31501 tctaaagaag aaatagacga tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg
    31561 atattcaaac gagtaggttt ggaataaaga agagtgagtt gcaaaattca attaaaatag
    31621 aatagcaaaa ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta
    31681 ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc
    31741 ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa
    31801 tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca
    31861 ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg
    31921 attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca
    31981 cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat
    32041 aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt
    32101 aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc
    32161 gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa
    32221 cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa
    32281 tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg
    32341 aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca
    32401 tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg
    32461 agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag
    32521 accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc
    32581 tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag
    32641 caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt
    32701 tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct
    32761 actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac
    32821 aacttgcaaa accggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa
    32881 ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg
    32941 gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat
    33001 gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt
    33061 tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc
    33121 gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca
    33181 aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg
    33241 aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat
    33301 tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat
    33361 caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt
    33421 gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac
    33481 ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct
    33541 atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga
    33601 ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga
    33661 cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca
    33721 aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg
    33781 aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac
    33841 ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca
    33901 gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc
    33961 atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt
    34021 ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag
    34081 ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc
    34141 gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt
    34201 ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc
    34261 ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg
    34321 gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc
    34381 cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg
    34441 ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga
    34501 aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac
    34561 cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg
    34621 caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg
    34681 gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt
    34741 ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg
    34801 atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg
    34861 attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa
    34921 tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa
    34981 ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg
    35041 ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag
    35101 tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag
    35161 tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga
    35221 aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag
    35281 aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa
    35341 tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg
    35401 ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg
    35461 cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta
    35521 gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt
    35581 ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg
    35641 cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg
    35701 atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata
    35761 aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga
    35821 cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg
    35881 tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca
    35941 tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac
    36001 tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg
    36061 ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga
    36121 cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag
    36181 cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa
    36241 agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg
    36301 gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag
    36361 tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa
    36421 ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca
    36481 gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt
    36541 ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa
    36601 tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt
    36661 ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga
    36721 ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt
    36781 aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat
    36841 cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat
    36901 tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg
    36961 aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat
    37021 ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa
    37081 tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc
    37141 taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat
    37201 cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag
    37261 agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt
    37321 gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt
    37381 gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc
    37441 atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat
    37501 tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct
    37561 gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt
    37621 caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa
    37681 agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa
    37741 atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca
    37801 agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg
    37861 gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg
    37921 gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg
    37981 actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct
    38041 tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg
    38101 tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc
    38161 ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa
    38221 aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat
    38281 ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc
    38341 agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg
    38401 agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag
    38461 gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat
    38521 ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac
    38581 aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta
    38641 tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg
    38701 gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta
    38761 taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc
    38821 gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga
    38881 tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt
    38941 ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa
    39001 agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt
    39061 gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc
    39121 tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat
    39181 tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat
    39241 tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat
    39301 ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca
    39361 ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa
    39421 ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa
    39481 acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca
    39541 cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag
    39601 gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc
    39661 aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc
    39721 ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt
    39781 gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt
    39841 tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg
    39901 atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg
    39961 tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat
    40021 cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa
    40081 ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac
    40141 gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg
    40201 attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc
    40261 atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta
    40321 gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa
    40381 aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg
    40441 gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa
    40501 gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt
    40561 gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag
    40621 acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa
    40681 caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat
    40741 gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc
    40801 atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact
    40861 gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt
    40921 gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg
    40981 gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc
    41041 agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt
    41101 atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga
    41161 ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct
    41221 aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac
    41281 gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc
    41341 ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca
    41401 gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag
    41461 tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg
    41521 atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc
    41581 ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg
    41641 gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt
    41701 gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg
    41761 cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa
    41821 gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac
    41881 aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt
    41941 ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac
    42001 ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc
    42061 aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt
    42121 tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc
    42181 tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct
    42241 tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa
    42301 aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg
    42361 tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga
    42421 tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc
    42481 tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt
    42541 tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt
    42601 gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt
    42661 agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta
    42721 ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca
    42781 accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg
    42841 gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta
    42901 ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg gattttttga
    42961 aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc
    43021 agctaaatat caaacgacac caactggctt ggcttctgct tggattttac gccatccagc
    43081 caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc
    43141 ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat
    43201 tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa
    43261 cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt
    43321 atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat
    43381 attcttttgg agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat
    43441 tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt
    43501 ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag
    43561 aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg
    43621 acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct
    43681 gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa
    43741 ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc
    43801 aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag
    43861 tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt
    43921 gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa
    43981 agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa
    44041 tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag
    44101 gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc
    44161 ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt
    44221 cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca
    44281 cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa
    44341 agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc
    44401 gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac
    44461 cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt
    44521 tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt
    44581 taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga
    44641 aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt
    44701 ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct
    44761 gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga
    44821 ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt
    44881 cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga
    44941 gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt
    45001 tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata
    45061 ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca
    45121 gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgcg
    45181 gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca
    45241 atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg
    45301 atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata atcattgata
    45361 tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa
    45421 aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa
    45481 gaaataatgg catccaaact gttataaata aaatggggag taatctgagc ttgcaataca
    45541 cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt
    45601 agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc
    45661 agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga
    45721 ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa
    45781 atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc
    45841 gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac
    45901 agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct
    45961 tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt
    46021 agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga
    46081 atatcattcc taagttcttc taggatattt tcctcattga actcatctac ggttacttgg
    46141 ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca
    46201 tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac
    46261 agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca
    46321 aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt
    46381 tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg
    46441 taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca
    46501 gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat
    46561 catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt
    46621 ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc
    46681 gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg
    46741 aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc
    46801 atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt
    46861 ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga
    46921 atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg
    46981 gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat
    47041 gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa
    47101 aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg
    47161 ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc
    47221 agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa
    47281 accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta
    47341 agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg
    47401 gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg
    47461 aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa
    47521 agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc
    47581 aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct
    47641 attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt
    47701 acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg
    47761 aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa
    47821 gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca
    47881 cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa
    47941 gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat
    48001 tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac
    48061 ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac
    48121 gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac
    48181 aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc
    48241 tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac
    48301 caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca
    48361 ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat
    48421 gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa
    48481 gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa
    48541 gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat
    48601 ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat
    48661 tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct
    48721 aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca
    48781 aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa
    48841 tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg
    48901 gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat
    48961 ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac
    49021 taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag
    49081 gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt
    49141 tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg
    49201 aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg
    49261 cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag
    49321 gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa
    49381 tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag
    49441 agcatattat tcgcgggatg gtgggcagag atttagttga tcgttatcct gaaagaaaac
    49501 cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc
    49561 acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa
    49621 tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt
    49681 atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg
    49741 ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac
    49801 tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc
    49861 aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga
    49921 tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac
    49981 aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc
    50041 caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg
    50101 cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt
    50161 gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg
    50221 cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag
    50281 gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat
    50341 ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg
    50401 atgacggatg gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc
    50461 catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg
    50521 tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc
    50581 caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca
    50641 tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga
    50701 ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt
    50761 ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc
    50821 cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa
    50881 tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc
    50941 ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct
    51001 tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt
    51061 ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg
    51121 gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga
    51181 gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa
    51241 gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact
    51301 tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg
    51361 aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg
    51421 atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag
    51481 aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct
    51541 aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca
    51601 ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg
    51661 gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca
    51721 ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg
    51781 tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa
    51841 aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt
    51901 agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt
    51961 tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga
    52021 ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat
    52081 cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata
    52141 tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt
    52201 ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac
    52261 aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc
    52321 gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc
    52381 aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga
    52441 ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc
    52501 tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca
    52561 acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat
    52621 ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa
    52681 aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga
    52741 aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt
    52801 tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt cagaataagg
    52861 gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt
    52921 ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg
    52981 gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga
    53041 gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg
    53101 caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt
    53161 ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa
    53221 ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac
    53281 tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta
    53341 tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt
    53401 gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac
    53461 aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt
    53521 gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga
    53581 gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca
    53641 ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa
    53701 gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct
    53761 acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat
    53821 cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa
    53881 gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg
    53941 gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa
    54001 ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg
    54061 gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa
    54121 gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca
    54181 attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta
    54241 gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat
    54301 ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat
    54361 tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta
    54421 aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata
    54481 cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa
    54541 aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg
    54601 taagtttgat tagaaaatag aaaaaatgca gatgaaaaat aaaacgcttt ccttattgtg
    54661 attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat
    54721 ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa
    54781 atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg
    54841 agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct
    54901 agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt
    54961 taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat
    55021 ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg
    55081 cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga
    55141 taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt
    55201 acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga
    55261 agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg
    55321 tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga
    55381 tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc
    55441 aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca
    55501 aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt
    55561 cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat
    55621 cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact
    55681 atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa
    55741 agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa
    55801 agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga
    55861 caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca
    55921 atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa
    55981 aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat
    56041 gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga
    56101 ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc
    56161 gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc
    56221 tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat
    56281 cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt
    56341 catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt
    56401 cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga
    56461 agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa
    56521 gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca
    56581 gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat
    56641 cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat
    56701 gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca
    56761 caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt
    56821 tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa
    56881 gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg
    56941 gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa attgccggat
    57001 atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg
    57061 atcaatgaag atgatacatt actagaagtt caaaatgaca aatcagtaga agaaatcccg
    57121 tcacctgtta caggaacggt taaaaatgtg attgttccag aaggtaccgt agcaaatgtt
    57181 ggggatgtat tagtagaaat cgatgcacca ggacacgaag acaatgaagg ggacagtggt
    57241 gtagccgcag aatcccaaac tccagctaaa ccggctgctg aacccactgt tgatacagaa
    57301 tcagctggat cgtcaagcga aggagtcttc caatttaagt taccggatat cggtgaagga
    57361 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
    57421 gatacattac tagaagttca aaatgacaaa tcagtagaag aaattccgtc acctgtcaca
    57481 gggacagtta aaaacgtgat tgttccagaa ggcactgtag caaatgttgg ggatgtatta
    57541 gtagaaatcg atgctcctgg acacaatagt gcaccgtcaa ctagtgcgcc atcagctgaa
    57601 gcaccaaaag aaaaagtgga aacatcaggt tcagcaagtg tcgttgaagc tgccgatcca
    57661 aataaacgtg tattggctat gccatctgtt cgtcaatttg cccgtgaaaa agatgtagat
    57721 atctcacaag taactgcaac tggaaaaggt ggcagagtaa caaaagagga catcgaaaac
    57781 ttcttggcag gtggtccaag cagtgcacca gcgaaatcag aagcgccaga agcggcagca
    57841 ccgaaagaag cagctcctgc agcagaaagc aaaccagctg caccagcaaa accattcaag
    57901 tccaacttag gagacttgga agaacgtgta gcaatgacgc caacacgtaa agctatcgct
    57961 aaagcaatgg tcaacagtaa gcatacagct ccacacgtga cattgcatga tgaagtagaa
    58021 gtgtctaaac tatgggataa tcggaaacga tttaaagaag ttgctgctgc aaatggtacg
    58081 aaacttactt tcttgccata tgtcgtgaaa gctttaacag cgactgttaa gaaataccca
    58141 gtattgaatg catctatcga cgatgcaaac caagaaatcg tttataaaca ttactacaat
    58201 atcggtattg caactgatac ggatcacgga ctatatgtac caaatgtgaa agatgcagac
    58261 cgcaaaggaa tgtttgctat tgcagacgaa atcaatgaga aagcaaaatt agctcatgac
    58321 ggcaaactat ctgcagaaga tatgcgcaat ggaacaatta cgatcagcaa tatcggatct
    58381 gtaggtggcg gttggtttac accagtcatc aactatccag aagtagctat tttaggtgta
    58441 ggaacgattg cacaacaacc aatcgtaaat gctgaaggcg aaatcgttgt cggtcgtgtg
    58501 atgaaactgt cattaagttt cgatcaccgt atcgtagatg gagcaactgc gcaacaagca
    58561 atgaataata tcaaacgtct tttagctgat ccagagctat taatgatgga aggatgataa
    58621 acaaatggta gttggagatt ttgctgtaga attagataca gttgtaattg gtgcgggacc
    58681 tggtggctat gtagctgcca tccgcgctgc tgaaatggga caaaaagtag caatcattga
    58741 aagagagtat attggcggtg tttgtttgaa tgtcggctgt attccttcaa aagcactgat
    58801 agctgcagga caccattatc aagagtcact agattctgaa atgtttggag taactagtga
    58861 aaacgtcaaa ctagattttg caaaaacaca agaatggaaa gaaaacaaag ttgtccatac
    58921 gttgacttct ggtgttggtt tcctattaaa aaaacataag gttgaaacaa tcgaaggaga
    58981 agcgttcttc gtagatgatc acacactacg cgtcattcat ccagattcag ctcaaactta
    59041 ttcgttcaat aacgcaatca tcgcaacagg ttcccgtcca atcgagattc caggatttaa
    59101 attcggagga cgtgtgttag attctactgg cggattagca ttgaaagaag tacctaagaa
    59161 atttgtgatc atcggtggcg gtgtgattgg tgccgaattg ggcggagcgt acgcaaactt
    59221 aggagcagaa gtaacgattc ttgaaggctc accacaaatt ttaccaactt acgaaaaaga
    59281 tctagtaaaa ttagtagaag atgatttcaa gaaaaaaggc gtaacagtcg taacgaatgc
    59341 aatggcgaaa gaagcagtcg ataacggtga cagcgtgaca gtgaaatatg cagtggacgg
    59401 gaaagaagaa tccgttactg ctgattatgt gatggtcact gtaggtcgtc gtccgaatac
    59461 agatgatatg ggcttagaac aagcgggtgt cgaagtagga gaacgaggat tgatcacggt
    59521 ggataaacaa ggacgtacga acgtacctaa catctatgcc atcggtgata tcgttccagg
    59581 ggcggcatta gcacacaaag ccagttatga agcaaaaatc gctgctgaag caatctctgg
    59641 caaaaaagta gctgtagatt ataaagcaat gccggcagta gccttcactg atcctgaatt
    59701 ggcttctgta ggtatgacga tcaaagaagc aaaagatgct ggtatcgaag caaaagcata
    59761 taaattccca ttttcaggga acggtcgggc attatcgctt ggtaaaacag aaggattcat
    59821 ccgtctagtt acgacgatcg aagataacgt gcttattggc gcgcaaatcg gtggagtcgg
    59881 cgcaagtgat atggtctctg aattagcttt agcaatcgaa tcaggaatga atgctgaaga
    59941 tatcgccttg acgattcatc ctcatccatc tcttggcgaa atcgtcatgg atgcttcgga
    60001 attagcatta ggtttaccaa tccatatttg atttgaataa tacaggcgaa acgcaactga
    60061 aaaatcagtt gcgttttgtt ttttagaatt atcaataata ttattatgta aacttaaaaa
    60121 tatgacaact tcttggttta ttaccacttc ctatgctaca ctgaagagga atatgaaata
    60181 ggagagttga agatgcaaga atatcagtat ccacttgatt tagattggac aacagacgaa
    60241 atggtcattg taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa agggataaac
    60301 aatgaagctt ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc cattggtgag
    60361 gaaaaacgat tgggacggga atttgaaaaa gcttccggtt attctttata tcgtacagtg
    60421 cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga taaatggaac
    60481 agatgattga tcatatcaag tcctggcttg aagaagctgg tgagaaaatc aaatcttctc
    60541 aacaaacatt aacaatctcg caaaaatcaa atcgaacgga tctagtaaca aatgtcgatc
    60601 gaaacatcca gaattttcta atcgaaaaaa tccattcctt tgatccagag gcgaaaatat
    60661 tggcagaaga agaagggtgt aatcaattat cggatctcga tggaagagtc tttatcatcg
    60721 atcctattga tggcaccttg aattttgtga ttgaagggga aaatttttgt attatgctcg
    60781 ctgtttatga atccggcgtt ggaaagcttg gtttcattta tgacgtgatg agaaatgaac
    60841 ttttttgggg tggaaaagga ataggcgtgt ataaaaatga tgagccattg cctagaccag
    60901 caaataaatc cttatcggaa ggcttacttg gtgtgaatag ttatcttttc ggtcataata
    60961 aatatggaat ccgagaaatt gcggaaagca gcatggggac acgtatgtac ggatgcgctg
    61021 gtcttgaact agtggcaatc ttgaaaggaa accacattgc ttatatttca aaccttagcc
    61081 cttgggatta tgcagcggga aatgtcttat tggaagagtt tggtatggta acaagcggtc
    61141 tttttggtac tcctttgaca tttgaaaaaa gggaatacta tttagcaggg acttcttgtg
    61201 catatgcaga aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt agaaatcatt
    61261 cacaaaaatg ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa aacattttac
    61321 tatatttcat gaaaaaagtc tgctataatt agcagtcgag taaaatttgc cctgtcataa
    61381 agaaccaata aaaaaggagt tagttacatt gaattataga aacgatatcc gtaacgtcgc
    61441 aattattgcc cacgttgacc acggaaaaac aacattagtt gatgaactat taaaacaatc
    61501 agatacatta gacgcacata cgcaattgca agaacgtgcg atggactcaa atgcgctaga
    61561 aaaagaacgt ggtatcacga ttttagccaa aaacacagct gtagactata aaggtattcg
    61621 cgtaaacatc atggatactc caggacacgc ggacttcggt ggcgaagtag aacggatcat
    61681 gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa tgcctcaaac
    61741 acgttttgta ttgaaaaaag cactggaaca acatatcaca ccaatcgttg tcgtaaacaa
    61801 gattgacaaa ccttctgctc gtcctgagca tgtcgtagat gaagttcttg aactattcat
    61861 cgaattagga gcagatgatg atcagttaga ctttcctgtg atttatgcat ctgcactaaa
    61921 tggtacgtca agcttgtcag atgatccagc agatcaagaa ccaaccatgg cacctatttt
    61981 tgatacaatt attgaaaaaa tccctgctcc tgtcgataac agcgacgaac cattacaatt
    62041 ccaagtttca ttacttgact acaacgatta tgtaggtcgt atcggaatcg gacgtgtatt
    62101 tagaggaaca atcaaagtag gagaccaagt agcattaatt aaattagacg gaacagttaa
    62161 gaaattccgt gtaacgaaat tatttggttt ctttggacta aaacgtctag agattcaaga
    62221 agcaaaagct ggagacttga ttgctgtctc aggtatggaa gatatctttg tcggtgaaac
    62281 agttactcct gtggatcatc aagatgcgtt acctatcctt cacattgacg aaccaacatt
    62341 gcaaatgact ttcttagtta ataattcacc atttgccggt cgagaaggta aattcgtaac
    62401 cgctcgtaag attgaagaac gtttgatggc agaacttcaa acagatgtat ccttacgagt
    62461 tgaaccaaca aactctccag atgcatggac agtttctggt cgtggagaat tgcatttgtc
    62521 tatcttgatt gaaaacatgc gccgtgaggg atacgaattg caagtatctc gtccagaagt
    62581 tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc aaatcgatac
    62641 accagaagaa tatatgggta gtgttatcga gtccttgagc ttacgtaaag gtgaaatgca
    62701 agatatggtc catacaggaa acggacaaat ccgtttgaca ttcttaacac ctgcccgcgg
    62761 tttgattggt tattcaactg aattcctttc aatgacacgt ggttacggaa tcatgaacca
    62821 tacttttgac caatatctgc caatgcttcc tggtcaaatt ggcggacgtc accaaggggc
    62881 gcttgtttcg atcgatacag gaaaagcaac gacttattct atcatgagta tcgaagaacg
    62941 cggtacagtc ttcgtagaac caggtacaga agtatatgaa ggaatgatta tcggggaaaa
    63001 cagccgtgat aatgatttga ctgtcaacat cacgaaagca aaacaaatga caaacgtacg
    63061 gtctgctact aaagaccaaa catctgtcat caaaaaacca aaacaattga cgttggaaga
    63121 atctttggaa ttcttgaatg atgatgaata ttgcgaagta acaccagaaa gcattcgctt
    63181 gagaaaacaa attttagaga aaaatgctcg tgaaaaagca tctaaaaaga aaaaataatc
    63241 tgtaaaagga tgaagagaga aaccagcttg ttataaaaaa tagctggttt ctttttttgt
    63301 ccaactcttt tctgtggaga agtcatcttt gtcaatggac ataaattcag aaaatacgcc
    63361 gaaagacgtt taaaagactt ttaaaaaact tatttctaga gagtttttgt ttcataataa
    63421 gataatgttt cacgaaagga gatcaacttg tccttcgaag aaaatcgaag taaaatggca
    63481 agagaaagga gcgggaagat gaacgactta ttaaaacaat actttggtta tgatgagttt
    63541 agacctggtc aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt attaggaatc
    63601 atgcctaccg gaagtgggaa atcaatctgt taccagcttc cagctttact attagatggg
    63661 cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga cgcggcgaat
    63721 caattaggta tacccgccac ttttatcaat agctcgttag atggttacga aacagctaga
    63781 cgcttccaag aaatcgatag acagcagtac cgtttacttt atatcgctcc tgaacgtttt
    63841 atcatgccgg attttatcca agcaatgaag cgatggaacg tccgtatgat cgctattgac
    63901 gaggcacatt gtatctctca gtgggggcat gatttccgcc caagttattt acagatggcg
    63961 aaccagctag atcaactgcc aaatcgtcct gtaattgttg ctttgacagc tacagcgact
    64021 gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa ccatattcag
    64081 actggttttg aacgagaaaa tctacgattt caagtaatta aagatcaaaa aaaagaacaa
    64141 tatctaatcg aatatttaaa gatcaacaaa aatcaatctg gtatcattta tgcagcaacg
    64201 agaaaagaag tagaccgtct ctatcatttg cttaaaaaat tcgatttttc cgtgggtaga
    64261 tatcatggtg gtctaaatga gaatgagcga acagaaatgc aagaagcctt tttatacgat
    64321 cgcttgcagc tgattgtagc gacaaatgca tttggtatgg gtataaataa aagcaatgtc
    64381 cgttttgtta tccattatca aattcctggc tctctagaag catactatca ggaagctggt
    64441 cgggcgggaa gagatggtct ttccagtgaa gccatcctgc ttttctctcc tcaagatatc
    64501 caagtccaaa agttttttat ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa
    64561 tatgaaaaat taaaagcgat gacagaatac gtgtatattg agtcctgtct tcagcaatac
    64621 atcctaaact attttggcga gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat
    64681 gacagagaaa tagtagaagt aacgacagaa gcacagatgg ttctttcttg cttaaaacgt
    64741 atgggtgaga actatggtaa acaaatgctg atgaaagtct tagcaggatc gaaagaacaa
    64801 aagctgcaag ctttaggttt tggacattta tctacctatg gactgatgaa aaaccaatcg
    64861 caaaaagaaa cgatgcagtt aattgaatat ttgatatcga atggctatct tctgaccatc
    64921 aatggtgagt atcctgtttt gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa
    64981 gaatctgttt atcgaaagga acccaagaaa gtccagcagc tatcggatga agaaaccgat
    65041 acactatttg aagtgctgcg ggaactgcga acggatctag catcagaagc aggcgttccc
    65101 ccgtatgtcg tattttcaga ttccacattg aaagaaatga gccgaatacg tccatcaagc
    65161 agattggaga tgcttcagat aaaaggagtt ggccaaagca agcttgataa atatggcgaa
    65221 gcctttttat ctcggataaa aaatgcggac tctaacgttc tggataagta gaaagaacga
    65281 atacgtaaaa atgaataaaa acaactggtt aaatctgttt tttttgctcc ttatgaaaat
    65341 caaatatgtt ataattaagg aattagaatg ggaggtaagc acagatggat tctttgtcga
    65401 aaaaacttgc gatccaagta ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa
    65461 atcaaaacaa cagtctctgt atctcacaat gcaaagcctt cgaagaagtg gtagatacac
    65521 agatgtacgg cttttcccgg caagtcggtt ttgctgtccg tataggagta atcggaaaga
    65581 acgagggcca gctgatgtta agtgagctag aacgagaatt gaatcagtta tatagtgagg
    65641 tttaccaaga aatttatgat aaaaaagaag ttggcaagga ggaatagact tgcctaagaa
    65701 agtgaaaaaa aggcatctat tagactatag catcttgatt ccctatttaa ttttatgtgt
    65761 attgggattg atcatggtat atagttctac gtcgtatcta ttattagaaa acggacaaaa
    65821 tccgtccgca tcagtcatta accagagtat tttttgggtg cttagcttga ttgctatcgc
    65881 actactttat aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca tggcagccat
    65941 cgcagttttg acgattttgc tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc
    66001 gaaaggttgg ctgcaaattg caggattctc gatccaacct gccgaatacc taaaaatcat
    66061 ttctatctgg tacctgtctc tgactttatc taaaagacaa aacagtgtac aaaaagattt
    66121 tcttgggact gtgaaacgtc cacttgcaat ggttatcgga cttactgcat tagtcgctat
    66181 tcttcccgat tttggtaatg cggcagttat ctttttgatt attttggttc tgcttttagc
    66241 cagcggagtc aattatgtgt atactttgat tgtaggagta ggaggctttt gtctaagtac
    66301 ttttacgatc tggctgatca atatcacaaa cggaaagatc ttacccggac gtttacagta
    66361 tatctataat cgatttgcta tttaccaaaa tcctttctca gacgaattaa ataaagggca
    66421 tcaactagtc aacggctact atgccatgtt caacggagga ctatttggca ggggattagg
    66481 taacagtatc cagaaaaaag gatttttaca agaagcacaa accgatttta tttatgcaat
    66541 cgtagtggaa gaactgggag tgattatggg gattttgata ttagcgttgc tattctttat
    66601 gattgtgcgc attgtccttg taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg
    66661 cattgggatc ggcgcaatgt ttttgatcca agtatttgtc aatcttggag gaattactgg
    66721 cgttattcct ttgacaggga tcacgttccc atttttaagt caaggaggct ccagtttgct
    66781 gatgctttcg atttgtgtcg gctttgtcct caacatcagt gcggatgaaa aacgcaagag
    66841 cttaggttta taataaaaaa agatgtagga gtggttcgat gaaaaaagtt ttagttgcta
    66901 acaggggtga aatcgctgta cgtgtatttc gtgcttgtac agaattagga attaaaactg
    66961 ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat
    67021 atttagtagg acaaggaaaa aaaccgattg atgcgtatct agatattgaa gggatcattt
    67081 ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa
    67141 acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga cccaaactgc
    67201 atcatttaga tatttttgga gataaaatca aagcaaaagc tgcagctatc gaagcgggga
    67261 ttgcttcgat tccaggcaca gatggaccaa tcgcttctat agacgatgcc ttggagtttg
    67321 cgaaacaata cggttatccg attatgatca aggcagctct aggcggcggc ggacgcggca
    67381 tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag
    67441 caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa atacatcgct aatcctaagc
    67501 atatcgaagt acagattctc ggggatacgc atggaaacgt catccatctt tttgaaagag
    67561 attgttctgt tcagcgccgt caccaaaaag tagtagaagt agcgccttgt gtctctatga
    67621 atgaacagca acgtcaaaaa atctgccagg cagctgtcca attgatgaaa tatgtcgggt
    67681 atgtcaacgc aggtacagta gaatttcttg ttgaaggaga tgatttttac tttatcgaag
    67741 tcaaccctcg cgtccaagtg gaacatacga tcacagaaat gattacagat attgatatcg
    67801 taacaacgca actgttgatt gctcaaggtt tggatctgca taaagaaatc ggattgcctc
    67861 agcaagaagg aattaaatta aatggttcag ccattcaatg ccgaatcact acagaagacc
    67921 cgttgaataa ctttttacca gacacaggga aaatcgatac gtatcgttca cctggtggtt
    67981 ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg
    68041 attcattgct agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa
    68101 tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt ccatttatgc
    68161 tcaatgtcat cacacatcct gaattccaat caggaaatgc aaaaacaacc ttcattgata
    68221 gtacagcaac attatttgaa tttcctcgac tacgagaccg cggaaacaaa acgatgaaat
    68281 atatcggtga aattactgtg aatggttttc caggtatcga aagtggcgaa aaaccatttt
    68341 atgaagaacc acgtatgcct aaagatctaa tcacaagaac tgattacgta acggctaaga
    68401 atgtattaga tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga caggaaaatt
    68461 tgttgttgac ggatacgaca tttagagacg cccatcaaag tctgctagca actcgtgttc
    68521 ggacaaagga tttcaagcag attgcacgtt tgactggaga aggtcttcct gaactctttt
    68581 caagtgagat gtggggggga gcaacatttg atgtggctta ccgctttttg aatgaagatc
    68641 cttggcaacg gttgagaaaa attcgttctt taatgcctaa tacgttgctg caaatgcttt
    68701 tcagaggttc aaatgccgtt gggtattcta attatcctga caatgtattg gtggaatttg
    68761 tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt ttgaactgga
    68821 ctccacaaat ggaaaaaagt atccaagctg tacgggatac tggtaaaatc gcagaggcag
    68881 ctatttgcta tactggcgat atcaacgatc caagtcgggc aaaatacaat gttcagtatt
    68941 ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca atcaaagata
    69001 tggctgggct gcttaaaccg caggcagctt atcgtttgat tagtgaattg aaagaaacga
    69061 cagatctgcc aatccatctg catactcatg ataccagcgg gaatggaatc atcacttatt
    69121 ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc aatgagtgca atgagtggga
    69181 atactagtca gcctagcatg agcagcttgt attatgcact ggtcaatggt ccacggctgc
    69241 cagaaatcac gattgaaaat gcacagaaat taaaccatta ttgggaagat gtgcgcatgt
    69301 attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa
    69361 tgccaggagg acagtattcg aatctacagc aacaggcaaa agctgtcgga ttaggtcatc
    69421 gctgggatga aatcaaacaa atgtatcata ctgtcaactt gatgtttggc gatatcgtta
    69481 aagtaactcc atcatctaaa gtagttggag atatggcact atttatggtc caaaatgatc
    69541 ttacagaaga agatatttat gaaaaaggcg aaacactaag tttccctgaa tctgttgtga
    69601 ctttcttcca aggagaacta gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga
    69661 ttattcttaa aggcagacct gcattgaatg aacgtccagg tcttttggct gaatcagtcg
    69721 acttcaacga agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca aaacaagacg
    69781 aagtattaag ctatttgatg tatccgcaag tctttttgga ctatcaaaaa gcatataatc
    69841 aatttggcga tgttacatta ctggacacac caacattttt ccaaggtata cgtttgggtg
    69901 aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat cattcgttta gatgaaatcg
    69961 gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg
    70021 aaatcactat caaagataat tcgatcatca gtacggtcca aacccgattg aaagctgaac
    70081 cgacaaaccg agaacaaatt ggagcaacga tgtctggttc tgttttggat gtattagtga
    70141 aaaaaggcga taacgttaaa aaaggtgata cgttgatgat tactgaagcg atgaaaatgg
    70201 aaacggccat cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg tcttcaggag
    70261 acactatttc gtctggtgac cttttgattg aagtgactga gaaataataa gcgaaaggtg
    70321 gggaaacatg aaaagactgg gtttgttttg gctgattctg ctattagtta ttgtggcatt
    70381 ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca
    70441 aatatcaaac caaatgacaa cgtggaaata ccaggagctt aaagcaactg gtttttctac
    70501 ttatatcgga aaaccgatcg atcagctgga aaaggatttt ggccaagcct atgatcgatt
    70561 aactagcgga tttggttttg aaacaagata ttatgaaaat aaagctagtc atctcacatt
    70621 tgaagcgaac atccaagaca gcagagtcac aactgttaaa gtactgcaaa cagggcctgc
    70681 tgatatcgcg ccgttttatt tagggatgac gatgcaagat cttacgaaaa tcactactat
    70741 ctatactaat ttcacttttg agtaccatga tacagaagtt ggaatcgaac taatggaaga
    70801 ggatatgaat tatcgtccgc taattgcttt tgataacgac acttttgcta ttttattttt
    70861 tgatcaaaca acaaacggcc tattttcagt tgtgtattta gataaagata gtcttttgaa
    70921 actattgcca tatcaagtgt ttggtgaagg ccttccacat tatcaagcag ataaatcggc
    70981 tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt acgactttat taaatatgct
    71041 tagaagagaa gacgaacttc ccatatacaa tcaaacactt gcctttcaaa accacagtca
    71101 gctgctacta catgattttt tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc
    71161 tgattggcaa aaatcgctag gcagtgctca aacgacagtg aaattctcac tgacgaatga
    71221 tgaattggag aagttaatca gtaaacaaaa attagaaaat actagtgggc tatttattca
    71281 tccggcgatt gacgctactt tttcgttttt atattggtac agcgatcctt acaaccatga
    71341 ccgtttcatg gacccgggaa cggattctgt aggcattgct ttttctaaag aaaatatgtt
    71401 agtcttattg caagaagaaa acggagaaag tagtgatagc agtgatcgtt aacgaagagc
    71461 tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag agtaaaacga
    71521 tggcttctta tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt tgccagttga
    71581 aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt agcggcatat ggtgagtatg
    71641 cacctgatta tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag cttgatatgg
    71701 atgagcatgt tgcagctttc cgatatagtg aaacacagct gcaaagtctg ttggatcaaa
    71761 ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa agtagatgca ggtagcccgt
    71821 tttttgaaac gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt agacaatagt
    71881 gaatttgaaa taacaaaacg acgcgggctg atcgtatggg tctacagctt aaaacaatta
    71941 aagaatctga aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa atatgtcgtg
    72001 ctttacttaa atgaagagac attggaagaa acagaagaac gaatccgtaa attacatttt
    72061 gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc cgaaaaaata
    72121 ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg aagagctaaa tacagaaatc
    72181 cgattagctg aacatcttta aaaatctctt atgagcagtt tttaaataaa aaataacttg
    72241 gataacgatc gatttttgat cattgtccaa gtattttttt ggtatactag taccagtacc
    72301 atttctaagg agtgagtaga tgcggataat atcaggaaat tatggcgggc gccgtttaaa
    72361 aagtttagca ggggcgaaca ccagaccaac tacagataaa gtaaaagaat ccatattcaa
    72421 tatgattgga ccatattttg atggagagac agtattggat ctgtttgcag gaagcggtgg
    72481 tttagctatc gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag acaaaaatta
    72541 ccaagcgcta aaaatcatca aagaaaacat cgaaattacg aaagaaccag agaaatttac
    72601 cacaataaaa cttgatgcgg acaaagcaat cgaacggcta gcagcggaga aacaacaatt
    72661 cgactacttg tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc aaatccataa
    72721 aatggaagaa ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa cggataaaac
    72781 agtcgaattg ccagaaactg tggctggatt ccatcaaata agaaaacaga tttatggcat
    72841 aacggccgta actatctata gaaaagaggg aaatcaatga aacgtgcatt attcccagga
    72901 agttttgatc cttttacaaa aggtcactta gatacggtgg aacgtgcggc taaattattc
    72961 gatgaggtag taattggtgt ttttatcaat acttcaaaga aaagtttgtt tcccccagaa
    73021 gaaagaatga cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa agtcatgcat
    73081 caagaaaatc aattgacggt agaaacagca aaagagattg gtgcagatgc gttgatcaga
    73141 ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga ttgctcagat gaatcatcat
    73201 ctatatgatg aattggaaac tgtcttctta ttagcaaaac cagaatatag tcacgtgagt
    73261 tccagtattt taaaagaagt actgcacttt ggcggagatg tgagcagcta cttaccgcca
    73321 gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg aaagctaaac aatggttgaa
    73381 aacagtactg attatcatat ttggaatgtt gatcattgga tttgtagtag ttcccttgcc
    73441 gtattatgta gaatcaccgg gagccacgat cgatctgaaa gaactaatta cggtcaatga
    73501 taaggaagat aaaggttctg ggtctttctc tcttacctct gttggtatca ggcaagctac
    73561 aggtttctta gctttaaaag ctaaaatgac cccttttgaa gaattgatca gtgaagaaga
    73621 attgactggt ggggcgacaa atgaagaata taatcaaatg caacagtatt atatggattc
    73681 ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt ataaaatgga
    73741 attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg aatttctttg gcaagctttc
    73801 agttggagat acagtaacaa aagtagacgg gaaaagcttt caaagtacag aagcatttat
    73861 ggattatgta aaaagccaaa aagttggaca aacaatcgaa atcagttatt tgcataatgg
    73921 tgaagaaaaa acagcttctg gtgatctgat cgagttacca acagataaaa aggcaggaat
    73981 aggtatcacg cttactgacc ataccgagat cgaatcagat acagatgttc gtattaattc
    74041 tggctctatc ggtgggccat cagcgggatt gatgttcact ttagaaatct atgaacaagt
    74101 cactggtaaa aacttgcgac atggtaagca aattgcagga actggaacga tcaacagtga
    74161 aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg ataaagctgg
    74221 cgtagagatt ttctttgcac cagatgatac gatttcagac gatgttaaga aagaatatcc
    74281 tgaaataaaa accaactacg aagaagcaaa agctgccgct aaaaaattga attcctcaat
    74341 gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac tatctagaga aaatgaaatg
    74401 aaactaaata attaaagagg ggttgtgaca cgagttctgt cacaacccct ctttttcgtc
    74461 aaaaatcttc acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta gatgttgaag
    74521 ggacgatttt gccagagctc actgtttctt caattttaaa tgaaaggagt aatgaatgat
    74581 ttttactggt cattctttga aaaaagaaca atgatagaaa aaatcagcaa gaaaatacta
    74641 gcttcaccca aattgatcat ttggatgtct atgatagttc ttatcttttg tatcgcaagt
    74701 ggttttttat tatcaaattt gctattttat agagaaacag aagagactgc tgcaaaagaa
    74761 atactttctt cgtttagttc cagtggagaa gatccatcag ctgaaggaaa cgatgtagaa
    74821 gaggatgctc gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa ggagcctggt
    74881 atctattcct tttcttcaga agaacgggtc tatgatgtac taaagcgtgc aggtggatta
    74941 ttagaagaag cggatagcga tcgaatcaac ttttctgcga agatagaaga tcagcaagtc
    75001 ctatatatcc ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt ggcatcctcg
    75061 gaaggaaaac agcctacagc agattccgag cctcccaaaa taaatatcaa tacagccact
    75121 ccatcagaac ttcagcagat tcctgggatc ggatcagtca aagcgcaaga aatcattcga
    75181 tttagagaag aaaacggatc cttccagaaa gtggaagacc tgcaggagat ttctggtatc
    75241 ggtgaaaaga cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa tgattcaaaa
    75301 tcgactggca cgagcaataa atggacgcta atggatacta agaataatcc aattctaaga
    75361 gcttggcagg acgaggaggt agtatttttg gataggaaat agcttgattg ttcgcaaaga
    75421 aacattaaaa aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg ggattcatat
    75481 tatgaatacg gctgtcagcg gcaataacag tcaagctcta cgagaacgct ttgccaaaga
    75541 tgtacttagc tatacagcag ataaagtatt tattctcatt gggacgaatg atctagcaga
    75601 gcataaacaa ctttctaaag aaacgtacca aaaaatctgc agtggatgat agttcagctg
    75661 aaaaaagact atagttctga tcatgctttc tttatcacgc caccagcagt cgatgagaca
    75721 aaacaaaaat atcggaataa caagttgatc aaagaatata tctctgttct cacagaagta
    75781 gcaaaacgcc atgattgtga agtatcgatt tttatacgga atttcttgaa caaatggatt
    75841 cgaagaattt ttataatggg ctaatggatg acggacttca tttcggcagt gaagggtagg
    75901 atcttttgtc agacaggatg attcgttata tttaaagaat attttgttta agcaatgagt
    75961 actttttaat caacttgaaa aaacggagga atataaaaca tgttagaaaa atcttcaaaa
    76021 gcatgcgata cgaataaaga aaaagttact gataagtcac ataatgaacg cattccttgg
    76081 gaccagtact ttatggctca ggcagtactg ttatctttac gtagtacgtg tacacgttta
    76141 gaagtaggag cgacactcgt tcgagaaaaa aggatcattg ctggcggcta taatggagca
    76201 gtcagtggag atgtccactg catagatgaa gggtgttacg tcgtggatgg acactgcttg
    76261 cgaacgatcc atgcagagat gaatgcactc ctacagtgtg caaaacttgg tatcccgacg
    76321 gaaggttcag aaatctatgt cacacatttt ccatgtttgg cctgtacaaa ggctttgttg
    76381 caagctggtg tgaaagggat caattatctc catgactatc gaaatgatcc ttatgcccaa
    76441 gcgttgatcg aacaattagg tgtatctgta caccaagttg ctttagactc acagcatttt
    76501 tcaaaacttc aaaatgaatt aacaggaaaa catgtctagc gttttagaaa ggcatgttcg
    76561 aaatcggttg atttttccgg caattacgtc gatgattatc tgtttgttgt attacactga
    76621 caattggggt tatcgtttgc ttcttgccgg aattttttta tggttttcgc taaaagaagg
    76681 acgttttatc gcgtacttgt gtattttgtg tagcataacg gtatccattt cttataggct
    76741 acaagagtat tcatttttag aggaaaaaac aacatctgcg caaattactg tatttacaga
    76801 tacgattcgt gcaaatggta attttttgac aatggaagga aggttatcag agaataaaca
    76861 aaagctccag ctttcttatc aagtaaacac agaagcagaa ttgaaaacat ggcttaactt
    76921 ccgaggaaag ggaatagaat tgactgctga aggcgagttc atctctccag caaaaaagcg
    76981 gaatctttat aattttgacc aagaagctta cttcaggaac catagaattt ctggacggtt
    77041 ccaaattcaa aagattgaac agatcaccat gcaaaatcac tggcgaaact ggcttcagag
    77101 aaaaagagga tcttttatct cctttgtcca atctgaattt ccgcaaaaaa caagtgtata
    77161 tattaattct ttattatggg gctataaaga tgcaggattc agagaaccag aagaaatttt
    77221 tagggagagt ggtttgctgc atctgttcag tctatcgggt ctacacatcc aattttattt
    77281 agggtggctg tattatttat ttcgacgtat cggatggcca ctacacgtca gtattattcc
    77341 gttgagtcta ctaactatct gttatgtttc tattgcagga agttctattt ctgttttgcg
    77401 tgctagtttg ctgtttttat tgcgattgct agtcaaattg ctgaataagc attattccac
    77461 catggactta tttgcagttg tcttttggct tcttttacta tttgatcctc gatttctttt
    77521 tcatgcgggt ggacagctta gttttttcat gagttttttg tttatcctga tttcttttga
    77581 tggagattgg ttgaaaaaaa cggcctctca cgtcctgttt actatgatca cgatgccgtt
    77641 gattgtatgg catttttatg agtggcctat tttaggtagt ctttttacat tactgattgc
    77701 accggtattc aagtttcttt ttcttcctgg tgtgttattt ctcacactct tcaatcgctg
    77761 gctgccggaa actttacttt tgctgataga agagggatta atcttatttg agcgaataat
    77821 cgatttttct tcagggtcgt ctctcattat aggacgtttg ccccttgtgc tatctgggat
    77881 gtatattttc ggattactga tttgtatgga taggttgaaa gaacaacctg tgaaacactt
    77941 gttttatgtt ggtctttttt cttttatttt tttgactttg ccatttttac gcttttcttc
    78001 aacgatcgct tttatcgatg tcggtcaagg agacagtatc tttttgcaaa gtccttttcg
    78061 aaaagaaacg attttgatcg atactggagg tcagttacac tttgaaagag aaaagtgggc
    78121 gcaaggtatg atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa aaggtcaggg
    78181 gataagtgta atcgataaac tcatcttgac acacgacgat acggatcatg tgggagaatt
    78241 acccacgtta tctcagcatt ttactatcaa aaaaatttat atcggttggg gcgctggacg
    78301 aaatgagcca ttacaaaggc atttatctga agctcaaaaa aatggtactg agatcattga
    78361 aatcaaacat ggggacagga tcagcggtta ttatgatctt tatgtattga ctccttttga
    78421 aaagggagag ggaagaaatg aagattcgat tggtctatgg atggaatata acaataggcg
    78481 ctttttattt ttaggagatt tgaatcaagc aatggaaaaa caattgcttt tgatttatcc
    78541 taatctgaaa gccgatgtag tcaaattagg tcatcacggt agccgtacgt caagtaacac
    78601 agattttctt tcccatattg aagcaaaaca tgggattata tcctgcggcg taaataatcg
    78661 ttatggtcac ccgcacagcg aagtattgga gacactagaa gaaaatcaga taaaaacatg
    78721 gcgaacggat caacaaggaa tgatcaccta tcgctggcat ccagtttttc atcctcatgg
    78781 gctagtggaa acaatgatag actagtaatt ctcatctgct catggtagga taatttttga
    78841 aagtggtgag taatttgagt atacaagcag agttacaaaa aatacgtaaa gaaaaacttg
    78901 catcttgtta cttgatttta ggaacagaaa agtttctcca agatcaagtt cggacagaaa
    78961 ttgtcaaaaa aatccaatta gagggcgaag atgacttaaa ttttttaagt tttgacatgg
    79021 aaaatgcaag tttagatgag gtagtagctg aagcagagac gttgccgttt ttcggagatc
    79081 aacggttagt attcgtagag aatccttatt ttttaacagg tgaaaaagta aacaatggaa
    79141 tcgaacaaaa tactgattta ttgacggctt atttgaaaga accattagaa tcgactgtat
    79201 tagtattctt tgcgccatat gaaaagttgg atgaacggaa aaaagtaacg aaacaattga
    79261 aaaaaacagc aattatcatc aatgtccaac agttgaatga aaaagaagta cgtcaatatc
    79321 tgatgaatac cttagagaat tcctcaatca aaatggaacg aagtgcaatt gatttattct
    79381 tgaggttgac cgacttagat ttatccaaaa tgatgagaga gttacagaaa ttggtccttt
    79441 atggacaaaa tcaacagcaa atcacggtaa gagaagtaga agaattagtt cctaaaacgt
    79501 tagaacataa tatttttgac atgacccaat atattttaaa agggaagacg gaacaagctt
    79561 tgcgtttata cgaagattta gttatgcaag gggaagaaac aatcaaaatt aatgcgattt
    79621 tgctttctca attacgttta tttttacaga caaaatttct tgtaaagatt ggttatcagc
    79681 aggcaaatat tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt gccatgcagg
    79741 aagtaaaaaa attcgatgaa catttgctgg tacagttgtt tgatcaatta gttgagatgg
    79801 attatcaaat caaaacggga caaatcgaaa aagaattaag ttttcaacta ttcgttttgc
    79861 gcacagggca gttgttaaga aatcgataag aagaaagacg agggcaaagc atgcgggaag
    79921 gtcgtttgtt tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc gattctttta
    79981 tttagcgatt tttttgctta aacgagattt gtcgcggtta gctttgtttt tgtggattag
    80041 accttttgat tcagccatat caatagcttt aacagcttct ttatataacg catctacgtt
    80101 gtcagcacct gaagctacag catcttcaaa ttttttgatt gcagtacgca tagcgcttgt
    80161 ttgagatgaa tttttcacat tagcgttttc gcttgtacga acacgtttga ttgcagattc
    80221 aatgtttggc attagtttca cctccgataa cttaagttct tacgcgtgtt accgcataat
    80281 tcaacattca ttattatacc taaaggcagc gtgctttgca atatgaacat gacaagtttt
    80341 tttccttaac agtcttttat taggattttt tttcagtaaa aacggatagt acaagctgaa
    80401 aaaaatcaaa aagacgctat tgtgggaggc aatagcgtca aaccaaacta gatttttatt
    80461 tatctattta aacaaagttt gaatgggaat gtttggtact aagttattat ataacaaaga
    80521 gaaccgtttg caaagttttt tttgaaaagg gatacgattc cctgttgttc tttgtttttc
    80581 tattaagaaa ctgcagttat tttataacca gtatcctttg atcagtaaag gctattctgg
    80641 tgtaccgatt attttaatgt attcatcgat attttttatc aacatttttt ttatgaccaa
    80701 ctggttccga cggttccatt tgccacttac ttccagcaca tatttattta ggggcaaacg
    80761 taagatatat tttgccaaat agcgatcttt aagcaggcca ttgatcacac ggtcctcaag
    80821 ctgcaagttg atcccagtca gcatcattgg ctgttcttgt aaaacgtacg tatcaattaa
    80881 aagaccttca aaagtttcca caagcgtctt cctttctttt caaagtaacc tgaaaacagg
    80941 cactaattct tccattttga tattcgttga agtggatgcg atcattcttt cttttgtcca
    81001 ctgtaatcta gtataacaaa aaagagcaga agagccaaaa agaaaaaaga gcatcgaatt
    81061 ctataggaag tctcattggc acggcctaaa ttattacaaa acattgatta aaaaaagccc
    81121 ctgcatcgca ggagcatccc ctatatcgtt ttaataggtc aatcaaaaag cagtatcaaa
    81181 cacattttgt atcaaatcag gaaataatag ataagctaag cccagaatca aaagtaccgc
    81241 tagaagaaac cagccgattg ttcctatgaa atactttaat ttttcgaaca ttgtatcacc
    81301 tcgttgtcag tatatcacgg atcagtaaat tgggggtgtt aagaatctcc taaaatagac
    81361 aaaatttgtt ctattttcct aaagcaaata cttgtaagat acagtaagtg ataccagaag
    81421 caatgaccgg tccggcggcg attcctctta agaagacaac acctaaaatc gtgccgaaaa
    81481 caagtgcaac agtgatttgt ggatcagaag caatcaatcc tacacccttg gaagataaga
    81541 cagccactag cccaccgcaa aaaacagcta cccagccaat aggggattta aaggcttcta
    81601 atagatcacg tagaccgatt tctcctgttg cgatagggat tagaatagcc gctgtgatga
    81661 tcgtcacccc aatattgatt cctttgcctt gaagccaagg gaaaaaggaa cgggaggctg
    81721 gtatgagctt cataatcaaa acaaaagctg ccgctatcat cagactttga tttttagcta
    81781 aaattgctac aagtaaaata attcctaaaa aaagccaact ttccatgaaa tatattcctc
    81841 actttcttcg aaacaatcat aacatgtttt tagaaagtta caaaagtagg aattcatcct
    81901 tcaagaccta ttcataagct gtttttttca taaaataaac aaaatcataa aaaaacactt
    81961 tgcttatttg acagaagaca gccgcgcttt gtatagtgga caagacttac atttttttga
    82021 agaatcagaa agatcaggta aaggtcttag taaacgcaag ccattcagaa taaccaatat
    82081 tgtgcttcct tcgtgtccta caactccaag aggtagattt aaaaattgca ggaagttaga
    82141 agcaatcaac aagagaataa caagaatcga aaaaataata ttttgtttga tgatccgccg
    82201 catttttaaa gatagccgat gactgtagac aaatttattt aagtcgcttt tcattaagac
    82261 gatgtctgcg atatccatag cggcatctgt cccttgcccc atagcgacac ctaaagaagc
    82321 ggctgctaaa gcaggcgcat cattgactcc atcaccaacc atcgcattta cttggtaaag
    82381 ttcattctct tttttgacta gttgtgtttt ttcttccgga gtacagcttg catagtaatg
    82441 ttcaatccct acttgttcag caatggcctt tgctgtgcca gaatggtcgc cggttatcat
    82501 acttgtatgg atgttttggg atttaaagta ggaaattact tgttgggtat ttgcttttgg
    82561 aatatctagc agacctaaaa cagcaattaa ctgctgatct ttagaaagat aaatcacggt
    82621 tttgccttga ttttccaaac gttcgatcat ttccgcaatc tctttgctga ttatcatcgt
    82681 ctcaggatca tatgcgtgct tgccaacttt ccattgcgca cctaaatagg tagtttgtaa
    82741 tccaaaacca gtaatttctt ctattggtaa gtattggatt tcttcaggta ttgtgcaatc
    82801 gaaatattgc aaaatcgcat gagcaagtgg atgcgtcgtt ttttgttcca tagcaacaag
    82861 aagttgttgt gccaaatcct tttcatcaaa gaaaaagctg tcagttacaa ctggttttcc
    82921 tttagtgagc gttcctgttt tatcaaaggc aatcgctttt aattcagaaa gcttttctaa
    82981 atggataccg cctttgatta aaatcccatt ttttgcagaa tttgaaagag cagctagaga
    83041 agctggtgta gcagatgcga caagcgcaca aggagaagcc acaactaata ataccattcc
    83101 tcgataaaag ctttcatccc aactccaacc taggaaaaag tgggggagaa ggatcattag
    83161 aggaacagca agcaaaatca attttacata attattttcg attcgttcga taaatgaagc
    83221 tgtttgagtt ggcgtattct gcgcttcttc tactaattgg ataatcttag caaagacagt
    83281 ttcggaactc gtttttgtta ctcgaatcgt gatgggattt ccttgattca gcgttcctgc
    83341 atacatctca tcgcctaaat atttttctac aggcacagat tcaccgctta atgcagactc
    83401 atcgatcgtt gcctgtgtgc tttctaaaaa gccatctatc ggaacagctg ctcctttagc
    83461 gacaaatacc aagttatcca cctttagttg agaaacatct acttcttgca tttgtccatt
    83521 ttcttttaat aattgggctt tttgtggttg aagattcatc aaagcagtaa tttctttttg
    83581 acttttatta gttgtgtatt cctctaatgc accgcttaaa caaaagatga atgtcaacat
    83641 tgctccttca aagtaattac cgatcaaaca ggcaccaata gcagctaaag ccatcaataa
    83701 atccacattc agtgtccgat cattccacaa ttctttcaag ccttccgatg tttgtttata
    83761 tccaccgatg ataatggcac tgatataaaa aagtggggtg atggtaagag agaatcggtc
    83821 gaagataaaa ccgaagatca taaataaaag gcagccgatt gttgctgcaa aagctttttt
    83881 ctgattagtc atcgttatta gacctccttg tcaaaacaag gatagcataa taaaaatgta
    83941 attgataata ataacagcta aatgagaacg ataatggtta tcatttggtg attaagcttg
    84001 atgttgtttt tattatttat aatgattatt attaaagaaa ataaaaaacc agaacatttc
    84061 tcaaataaga
//
LOCUS       DAZKBH010000003        80178 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000003 DAZKBH010000000
VERSION     DAZKBH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.003"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..446
                     /locus_tag="ACG8OO_000192"
     CDS             210..446
                     /locus_tag="ACG8OO_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044132.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            453..2405
                     /locus_tag="ACG8OO_000193"
     CDS             453..2405
                     /locus_tag="ACG8OO_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9044133.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2477..2974)
                     /gene="dfrG"
                     /locus_tag="ACG8OO_000194"
     CDS             complement(2477..2974)
                     /gene="dfrG"
                     /locus_tag="ACG8OO_000194"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9044134.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3249..4376
                     /locus_tag="ACG8OO_000195"
                     /pseudo
     CDS             3249..4376
                     /locus_tag="ACG8OO_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            4474..4662
                     /locus_tag="ACG8OO_000196"
     CDS             4474..4662
                     /locus_tag="ACG8OO_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP9044135.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4720..5073)
                     /locus_tag="ACG8OO_000197"
     CDS             complement(4720..5073)
                     /locus_tag="ACG8OO_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9044136.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5604..6026
                     /locus_tag="ACG8OO_000198"
     CDS             5604..6026
                     /locus_tag="ACG8OO_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9044137.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6023..6253
                     /locus_tag="ACG8OO_000199"
     CDS             6023..6253
                     /locus_tag="ACG8OO_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9044138.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6751..6951
                     /locus_tag="ACG8OO_000200"
     CDS             6751..6951
                     /locus_tag="ACG8OO_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFP9044139.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            6978..8171
                     /locus_tag="ACG8OO_000201"
     CDS             6978..8171
                     /locus_tag="ACG8OO_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9044140.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8239..9804)
                     /gene="guaA"
                     /locus_tag="ACG8OO_000202"
     CDS             complement(8239..9804)
                     /gene="guaA"
                     /locus_tag="ACG8OO_000202"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9044141.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9863..10786)
                     /gene="coaA"
                     /locus_tag="ACG8OO_000203"
     CDS             complement(9863..10786)
                     /gene="coaA"
                     /locus_tag="ACG8OO_000203"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9044142.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10982..11587
                     /locus_tag="ACG8OO_000204"
     CDS             10982..11587
                     /locus_tag="ACG8OO_000204"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9044143.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11895..12911
                     /locus_tag="ACG8OO_000205"
     CDS             11895..12911
                     /locus_tag="ACG8OO_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9044144.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13052..14353
                     /locus_tag="ACG8OO_000206"
     CDS             13052..14353
                     /locus_tag="ACG8OO_000206"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFP9044145.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14399..15061
                     /gene="deoC"
                     /locus_tag="ACG8OO_000207"
     CDS             14399..15061
                     /gene="deoC"
                     /locus_tag="ACG8OO_000207"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9044146.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15082..15480
                     /locus_tag="ACG8OO_000208"
     CDS             15082..15480
                     /locus_tag="ACG8OO_000208"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9044147.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15546..16628
                     /locus_tag="ACG8OO_000209"
     CDS             15546..16628
                     /locus_tag="ACG8OO_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9044148.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16840..18402
                     /locus_tag="ACG8OO_000210"
     CDS             16840..18402
                     /locus_tag="ACG8OO_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044149.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18395..19528
                     /locus_tag="ACG8OO_000211"
     CDS             18395..19528
                     /locus_tag="ACG8OO_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9044150.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19528..20484
                     /locus_tag="ACG8OO_000212"
     CDS             19528..20484
                     /locus_tag="ACG8OO_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9044151.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20583..21749
                     /gene="deoB"
                     /locus_tag="ACG8OO_000213"
     CDS             20583..21749
                     /gene="deoB"
                     /locus_tag="ACG8OO_000213"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9044152.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21773..22591
                     /locus_tag="ACG8OO_000214"
     CDS             21773..22591
                     /locus_tag="ACG8OO_000214"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9044153.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22608..23315
                     /gene="deoD"
                     /locus_tag="ACG8OO_000215"
     CDS             22608..23315
                     /gene="deoD"
                     /locus_tag="ACG8OO_000215"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9044154.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23613..24299)
                     /gene="gpmA"
                     /locus_tag="ACG8OO_000216"
     CDS             complement(23613..24299)
                     /gene="gpmA"
                     /locus_tag="ACG8OO_000216"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9044155.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24471..25052)
                     /locus_tag="ACG8OO_000217"
     CDS             complement(24471..25052)
                     /locus_tag="ACG8OO_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9044156.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25185..25868)
                     /gene="rpiA"
                     /locus_tag="ACG8OO_000218"
     CDS             complement(25185..25868)
                     /gene="rpiA"
                     /locus_tag="ACG8OO_000218"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9044157.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26187..26600
                     /gene="rpsL"
                     /locus_tag="ACG8OO_000219"
     CDS             26187..26600
                     /gene="rpsL"
                     /locus_tag="ACG8OO_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9044158.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26684..27154
                     /gene="rpsG"
                     /locus_tag="ACG8OO_000220"
     CDS             26684..27154
                     /gene="rpsG"
                     /locus_tag="ACG8OO_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9044159.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27248..29335
                     /gene="fusA"
                     /locus_tag="ACG8OO_000221"
     CDS             27248..29335
                     /gene="fusA"
                     /locus_tag="ACG8OO_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9044160.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29507..30694
                     /gene="tuf"
                     /locus_tag="ACG8OO_000222"
     CDS             29507..30694
                     /gene="tuf"
                     /locus_tag="ACG8OO_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9044161.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31101..31409
                     /gene="rpsJ"
                     /locus_tag="ACG8OO_000223"
     CDS             31101..31409
                     /gene="rpsJ"
                     /locus_tag="ACG8OO_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9044162.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31440..32069
                     /gene="rplC"
                     /locus_tag="ACG8OO_000224"
     CDS             31440..32069
                     /gene="rplC"
                     /locus_tag="ACG8OO_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9044163.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32097..32720
                     /gene="rplD"
                     /locus_tag="ACG8OO_000225"
     CDS             32097..32720
                     /gene="rplD"
                     /locus_tag="ACG8OO_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9044164.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32720..33010
                     /gene="rplW"
                     /locus_tag="ACG8OO_000226"
     CDS             32720..33010
                     /gene="rplW"
                     /locus_tag="ACG8OO_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9044165.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33049..33882
                     /gene="rplB"
                     /locus_tag="ACG8OO_000227"
     CDS             33049..33882
                     /gene="rplB"
                     /locus_tag="ACG8OO_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9044166.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33925..34203
                     /gene="rpsS"
                     /locus_tag="ACG8OO_000228"
     CDS             33925..34203
                     /gene="rpsS"
                     /locus_tag="ACG8OO_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9044167.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34226..34573
                     /gene="rplV"
                     /locus_tag="ACG8OO_000229"
     CDS             34226..34573
                     /gene="rplV"
                     /locus_tag="ACG8OO_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9044168.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34587..35243
                     /gene="rpsC"
                     /locus_tag="ACG8OO_000230"
     CDS             34587..35243
                     /gene="rpsC"
                     /locus_tag="ACG8OO_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9044169.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35246..35680
                     /gene="rplP"
                     /locus_tag="ACG8OO_000231"
     CDS             35246..35680
                     /gene="rplP"
                     /locus_tag="ACG8OO_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9044170.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35670..35858
                     /gene="rpmC"
                     /locus_tag="ACG8OO_000232"
     CDS             35670..35858
                     /gene="rpmC"
                     /locus_tag="ACG8OO_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9044171.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35883..36149
                     /gene="rpsQ"
                     /locus_tag="ACG8OO_000233"
     CDS             35883..36149
                     /gene="rpsQ"
                     /locus_tag="ACG8OO_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9044172.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36207..36575
                     /gene="rplN"
                     /locus_tag="ACG8OO_000234"
     CDS             36207..36575
                     /gene="rplN"
                     /locus_tag="ACG8OO_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9044173.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36612..36920
                     /gene="rplX"
                     /locus_tag="ACG8OO_000235"
     CDS             36612..36920
                     /gene="rplX"
                     /locus_tag="ACG8OO_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9044174.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36948..37487
                     /gene="rplE"
                     /locus_tag="ACG8OO_000236"
     CDS             36948..37487
                     /gene="rplE"
                     /locus_tag="ACG8OO_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9044175.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37506..37691
                     /locus_tag="ACG8OO_000237"
     CDS             37506..37691
                     /locus_tag="ACG8OO_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFP9044176.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37727..38125
                     /gene="rpsH"
                     /locus_tag="ACG8OO_000238"
     CDS             37727..38125
                     /gene="rpsH"
                     /locus_tag="ACG8OO_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9044177.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38157..38693
                     /gene="rplF"
                     /locus_tag="ACG8OO_000239"
     CDS             38157..38693
                     /gene="rplF"
                     /locus_tag="ACG8OO_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9044178.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38854..39210
                     /gene="rplR"
                     /locus_tag="ACG8OO_000240"
     CDS             38854..39210
                     /gene="rplR"
                     /locus_tag="ACG8OO_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9044179.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39231..39731
                     /gene="rpsE"
                     /locus_tag="ACG8OO_000241"
     CDS             39231..39731
                     /gene="rpsE"
                     /locus_tag="ACG8OO_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9044180.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39746..39925
                     /gene="rpmD"
                     /locus_tag="ACG8OO_000242"
     CDS             39746..39925
                     /gene="rpmD"
                     /locus_tag="ACG8OO_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9044181.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39969..40409
                     /gene="rplO"
                     /locus_tag="ACG8OO_000243"
     CDS             39969..40409
                     /gene="rplO"
                     /locus_tag="ACG8OO_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9044182.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40409..41704
                     /gene="secY"
                     /locus_tag="ACG8OO_000244"
     CDS             40409..41704
                     /gene="secY"
                     /locus_tag="ACG8OO_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9044183.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41766..42413
                     /locus_tag="ACG8OO_000245"
     CDS             41766..42413
                     /locus_tag="ACG8OO_000245"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9044184.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42604..42822
                     /gene="infA"
                     /locus_tag="ACG8OO_000246"
     CDS             42604..42822
                     /gene="infA"
                     /locus_tag="ACG8OO_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9044185.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42855..42971
                     /gene="rpmJ"
                     /locus_tag="ACG8OO_000247"
     CDS             42855..42971
                     /gene="rpmJ"
                     /locus_tag="ACG8OO_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9044186.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42989..43354
                     /gene="rpsM"
                     /locus_tag="ACG8OO_000248"
     CDS             42989..43354
                     /gene="rpsM"
                     /locus_tag="ACG8OO_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9044187.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43382..43771
                     /gene="rpsK"
                     /locus_tag="ACG8OO_000249"
     CDS             43382..43771
                     /gene="rpsK"
                     /locus_tag="ACG8OO_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9044188.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43851..44789
                     /locus_tag="ACG8OO_000250"
     CDS             43851..44789
                     /locus_tag="ACG8OO_000250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9044189.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44817..45197
                     /gene="rplQ"
                     /locus_tag="ACG8OO_000251"
     CDS             44817..45197
                     /gene="rplQ"
                     /locus_tag="ACG8OO_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9044190.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45469..46308
                     /locus_tag="ACG8OO_000252"
     CDS             45469..46308
                     /locus_tag="ACG8OO_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9044191.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46284..47153
                     /locus_tag="ACG8OO_000253"
     CDS             46284..47153
                     /locus_tag="ACG8OO_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9044192.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47143..47940
                     /locus_tag="ACG8OO_000254"
     CDS             47143..47940
                     /locus_tag="ACG8OO_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFP9044193.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48143..48889
                     /gene="truA"
                     /locus_tag="ACG8OO_000255"
     CDS             48143..48889
                     /gene="truA"
                     /locus_tag="ACG8OO_000255"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9044194.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49222..51891)
                     /locus_tag="ACG8OO_000256"
     CDS             complement(49222..51891)
                     /locus_tag="ACG8OO_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFP9044195.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52129..52317)
                     /gene="rpmB"
                     /locus_tag="ACG8OO_000257"
     CDS             complement(52129..52317)
                     /gene="rpmB"
                     /locus_tag="ACG8OO_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9044196.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52587..52949
                     /locus_tag="ACG8OO_000258"
     CDS             52587..52949
                     /locus_tag="ACG8OO_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9044197.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            53001..54683
                     /locus_tag="ACG8OO_000259"
     CDS             53001..54683
                     /locus_tag="ACG8OO_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFP9044198.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            54800..56836
                     /gene="recG"
                     /locus_tag="ACG8OO_000260"
     CDS             54800..56836
                     /gene="recG"
                     /locus_tag="ACG8OO_000260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9044199.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            56885..57886
                     /gene="plsX"
                     /locus_tag="ACG8OO_000261"
     CDS             56885..57886
                     /gene="plsX"
                     /locus_tag="ACG8OO_000261"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9044200.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            58008..58250
                     /gene="acpP"
                     /locus_tag="ACG8OO_000262"
     CDS             58008..58250
                     /gene="acpP"
                     /locus_tag="ACG8OO_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9044201.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            58518..59522
                     /locus_tag="ACG8OO_000263"
     CDS             58518..59522
                     /locus_tag="ACG8OO_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044202.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            59523..60467
                     /locus_tag="ACG8OO_000264"
     CDS             59523..60467
                     /locus_tag="ACG8OO_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044203.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            60467..61429
                     /gene="opp4B"
                     /locus_tag="ACG8OO_000265"
     CDS             60467..61429
                     /gene="opp4B"
                     /locus_tag="ACG8OO_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9044204.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            61456..62370
                     /locus_tag="ACG8OO_000266"
     CDS             61456..62370
                     /locus_tag="ACG8OO_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9044205.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            62396..64177
                     /locus_tag="ACG8OO_000267"
     CDS             62396..64177
                     /locus_tag="ACG8OO_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9044206.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            64525..65211
                     /gene="rnc"
                     /locus_tag="ACG8OO_000268"
     CDS             64525..65211
                     /gene="rnc"
                     /locus_tag="ACG8OO_000268"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9044207.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            65231..68812
                     /gene="smc"
                     /locus_tag="ACG8OO_000269"
     CDS             65231..68812
                     /gene="smc"
                     /locus_tag="ACG8OO_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFP9044208.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            68821..69630
                     /locus_tag="ACG8OO_000270"
     CDS             68821..69630
                     /locus_tag="ACG8OO_000270"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9044209.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            69641..70639
                     /gene="ftsY"
                     /locus_tag="ACG8OO_000271"
     CDS             69641..70639
                     /gene="ftsY"
                     /locus_tag="ACG8OO_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9044210.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            70826..73093
                     /gene="gshAB"
                     /locus_tag="ACG8OO_000272"
     CDS             70826..73093
                     /gene="gshAB"
                     /locus_tag="ACG8OO_000272"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFP9044211.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(73182..73634)
                     /locus_tag="ACG8OO_000273"
     CDS             complement(73182..73634)
                     /locus_tag="ACG8OO_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFP9044212.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            73784..74734
                     /locus_tag="ACG8OO_000274"
     CDS             73784..74734
                     /locus_tag="ACG8OO_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9044213.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            74727..75686
                     /locus_tag="ACG8OO_000275"
     CDS             74727..75686
                     /locus_tag="ACG8OO_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9044214.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            75683..76438
                     /locus_tag="ACG8OO_000276"
     CDS             75683..76438
                     /locus_tag="ACG8OO_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044215.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            76461..77414
                     /locus_tag="ACG8OO_000277"
     CDS             76461..77414
                     /locus_tag="ACG8OO_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9044216.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(78058..78354)
                     /locus_tag="ACG8OO_000278"
     CDS             complement(78058..78354)
                     /locus_tag="ACG8OO_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFP9044217.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            78709..79065
                     /locus_tag="ACG8OO_000279"
     CDS             78709..79065
                     /locus_tag="ACG8OO_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9044218.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            79075..79974
                     /locus_tag="ACG8OO_000280"
     CDS             79075..79974
                     /locus_tag="ACG8OO_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9044219.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        26221 a        14426 c        17052 g        22479 t
ORIGIN      
        1 gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga atttgatact cgttttttat
       61 tgttaaatca acgtttgttg ttaatttaca ccacaaaatg taagttaaaa tgtatataaa
      121 aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg tgtgttaaaa tggttacaat
      181 ttactaagca aaaaggggtg cattttccta tgcaatttct tgagccaaaa aataagaacg
      241 ctaagtctgt ggattgggaa atatcagagc aagtaagggt gatagttaag caatacgctg
      301 aatatgcaga gcgtactgaa agcgaagcag tggatgaatt cttattgaac attttagacg
      361 ataagaagtt tattgaatgg attgccaaca agcgaagtaa taagcggatt gttgaaaaaa
      421 tgggtatcaa agatagagtg ggttgataga caatgaccaa gcttaaaaga ttagccacga
      481 aggaagatga aattgtagat gttaaaattc cgatttcaaa cgaagaatta aaagataggg
      541 ctaaacaata tgatttatta actccaaaac gatttgcaac aaggtataac aaaatgttgt
      601 tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc cattcaatat aattactgca
      661 tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa attcaaagat gtaaaaggga
      721 aaccaagtcg atataaaatg actggtagtt ccaaagataa aggtcataaa ggtatgtact
      781 gcaatgacaa tcctattggc agaggtgtat cgcaaaactg tacagtaacc cctttatcta
      841 attggtctgt tgttgaagaa attaaacgcc ttatagaaat caatagcatc caagatgttg
      901 agccagatta tcaatttcat aaagaaggct gctcggaaga agaatcaacc ccattcaatg
      961 agccgaagca attttacaaa agggggaaaa gtaggggcaa atctcaacgc tatcaatgta
     1021 aggcgtgtaa gaagttcact aatgttctac caaaaagaga agaaaccact acttatcacc
     1081 aacaaaagaa caccattctt ccaatgttcg ctaagatggt tgttggaagg gtttcagtca
     1141 gtcgtacttg tgatatttta ggaattggag ttggaaccta ctatcataag ctggagtggc
     1201 tgtataggcg ttgtttggag tttttagaac gatatgagac acaacccctt caaacaaaga
     1261 aatttaacga gatgtggctt aatacggaca agatgcacta ctacctaaat aacgtccgta
     1321 agaaaggaca aggttcaaag aaatatacgg ggtttgaaga ccttaatatg caaacgtata
     1381 tagttgtatc tgctgaggtc ttatcaagat atgtgttccg ttctgatgtt gcctatgatt
     1441 ggaatatttc aatggatgaa ttaaatgaag atacaagaaa attcaaagaa gaccatctca
     1501 atacttttag tcgtaaaaat gataggttag attggtctta ttatccacaa gaaccatccg
     1561 ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt agggaaaatt actaatagga
     1621 gtagatttgt cgatggttta aatgttgatg ctccttatac tacaactgct cactattggt
     1681 taattaaaca aatggttaat gccgacgaat ggcgaatgat tagtgatgat gacttctcta
     1741 ttagaaacgc cttttataga gtattcacga aagaattaag gttgagcgat gcacatcatt
     1801 ttatttgcca agttaacaag acgaaaagca gaaaacaatg cttaaaagaa ttcggacaag
     1861 caaaagcaga actgctcgat tggggagata tcagaggatt caaaacaaag tttctacgga
     1921 ctttggcatc tcattatcta actgaattac taacaagtca tcaattccac gaagaggcta
     1981 ttagtaaaga tggagaaaga tatcgcaaat atgccgataa ccctatcaaa catccattag
     2041 ccactaagga taaaggattt tattcagttg attgtagaac ggatttatct gctctggaac
     2101 caaacgaaat cgcaaagatg ctattaaatg ttaatgacca ttcgaccaat agctttattc
     2161 aacagataag acgttatata tcatctcttg aaagaccatt aacaacagct cgtggtgata
     2221 agaagagcta tatttacgct aacttcaatc cgaagtatgc tcaatttgcg attacaattc
     2281 taagaactta ctacaacttt tgtcgccctt ttaaatcagc ggataaaaag gttttgacac
     2341 ctgctcaaag attagggatt acagataaac aatttgattg gaaagatata atttatttca
     2401 aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc tttatcttta ctgttcttct
     2461 tcaattaacg cacccgttaa ctcaataagt tttttctttc atatacatga aaatagtagt
     2521 tatacggatt tttatcattc tttatccctt tttgggcaaa tacctcattc cattcctcat
     2581 aattcacttc tggaaaaaaa gtatctcctt cgaattcatg atgtattttt gtgatgtaca
     2641 ttttctcaac ataagggaaa aacaaattat aaatctgttc tcctccgaaa ataaaaattt
     2701 cttcttcgtt tttacataac tcaaaaacat cttctattga atgaacaatt tcacaaccat
     2761 taaaggtaaa ccccttatct ctcgtcagaa taatatttct tctgtcaggt aaggctcttc
     2821 cgattgattc aaggttcttc ctacctaata ttatcggatg tccctttgta gtatttttaa
     2881 catattccca gtccttggga atcctccaag gaatgtcatt ctctttgcca atcactctat
     2941 tcttatccat cgcagcaatc aaagaaactt tcatttataa cacagccttt ttaactaatt
     3001 taccatctaa tttcttattt ctatcaaaaa accttttctc attatcctgc ccgttagttg
     3061 aataagcaat tttttctata agttatatta tcataatacc acaaaatcac aaattttcat
     3121 tcaattatca ttttcaattg cattttataa atcattttgt atgtaatttg gtatgtaagt
     3181 taaaatgaaa aaagtagtcc attttgaagt acattttgtc attttgtact ccaaaactga
     3241 actacttact tgcatatata cgcctttata gtggagtact acatttacga gattcggtta
     3301 gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat
     3361 tatgtaagat tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga
     3421 agttaaatag tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc
     3481 cgcaccctct actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc
     3541 ttgatgccct tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta
     3601 cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac
     3661 tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg
     3721 agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct
     3781 gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga
     3841 gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac
     3901 gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa atctgtttta
     3961 agtacggttt atactatagc cctgttagta ctccagcaga ttttcggatg cttactccta
     4021 ttgtactgga gcaagccttt agaaaagctg gaacagaatt gttagagcca tatcttagtt
     4081 ttaaagttta tgcaccacag gaatatcttt cacgggcata taacgatgct cccaaatatt
     4141 gtgcaaatat cgtaaatact caactgaaaa ataatgaggt cattattatt ggagaaattc
     4201 ctgctcgatg tattcaagat tatcgcaatg atttaacttt ttttacaaat gggcttagtg
     4261 tttgtttagc agagctaaaa ggatatcagg ttaccactgg cgaacctgtt tgccagaccc
     4321 gtcgtctaaa tagtcggata gataaagtaa gatatatgtt caataaaata acttagtgcg
     4381 ttttatgttg ttatataaat atggtttctt attaaataag atgaaatatt ctttaatata
     4441 gatttgaatt aaagtggaaa ggaggagatt gttattataa actacaagtg gatattgtgt
     4501 cctatttgtg gaaataaaac aagactacga atacgagtgg atactatact taaaaatttc
     4561 cctttatact gccccaaatg taagaacgaa actttaatta atgttcaaaa aatgaatata
     4621 ataacaatca aagagccaga cgccaagacg cagagccgat aatttgagaa atgaaactct
     4681 catcttatcg gctctttttg tttatctgaa ttttactgac tagccttcaa tatttcttgc
     4741 ttcattgata ccttttgcta gtgcttccat taaggatagt tctttgtctg taaagctatc
     4801 catgtatttt tctacctgta accttcgagt gcttttaatc aaattatctg taggtaagaa
     4861 aaattcatca atagatacat ggagtaatga tacaagatca tacagcactt gtgtacttgg
     4921 gtgttgtcct ttgttttcta tatttgttaa ataacgtggg tcaatttcaa tcattgttcc
     4981 cacttgttca cgagtgagac cccgtttcat tcgagcctct ttaatcgcta aaccaacggc
     5041 tctaaaatcg tatttatctt cttttttacg catactaaat cacctctata cattttactg
     5101 ttcctatata accataaaca ggtatagaaa aacatgttat atagtttatg agttcctata
     5161 gaacactgtg gaatttatta atgcgatgaa tccaatttca aatagaaagg tcgctgattt
     5221 tatttaccta tacaacttta taaaatttag tcatactcct agaaagtaga taaaattggc
     5281 gacttaatgg agggaaaagt cgccatgaga aagataatct gtcatctagt tctcttaatt
     5341 aagttcttat caaaaaaact tatccacctg tactggatag atatgaccat caatgtggtt
     5401 ttgcagatta catagataga aaaatcaaag catatgaaca gagaaaacaa tctgtttatg
     5461 tgcttttttg gtcacttaat aaatatcttt taggatttct cttcatttat gcaacaaatc
     5521 ccccgttcgt gtcggggttt agtatgaagg gagataaaga agcacatgcc actccctttc
     5581 acgacagaaa gggggtgaaa agcatgaaac catcttcttt tcaagaaacc atagaaaatc
     5641 agtttgacta tatctgcaaa caagttattg aagatgaacg aaaagattac ttcaaacatc
     5701 tagacagact atcagcaaaa gaagtctcgt tttctgatat agataactac cttgtcaact
     5761 ctttttctac tgttgatacg tacgtcactg atttccaact atttaaatta tttggattaa
     5821 gaatcaatgt tgaaagtgat ttattaagtg aagccctgcg tcatctttca gagaaaaaac
     5881 ggaacattat tttacttcat tactttatgg atatgagtga tgtggagatt gcggagttac
     5941 taaatttgaa tcgttcgact gtttatcgtc atcgaataag tggactagcc atgattaaag
     6001 aattcatgaa ggagtgtaaa gaatgaaaac agcttatcca agggttccct tccccctcat
     6061 tgtaaaggcg acagatggcg acgtggaagc aattaatcag attgttaaac attatcgagg
     6121 gtatacctca aaacgttcat tacgtcgcat gacagacgaa tatggaaatt ctcatatggt
     6181 tattgatgaa accctacgtg gcagaatgga aacgaaactc atctctaaga ttttatcgtt
     6241 cgaaattaaa tagtcccatt ttttcgtgga agcgtgtcga atcgtatcca cgcttcccga
     6301 tttgggaggt ttgttattcc atcaaagcat attgtgtgtt cagtaggttt tgataggcta
     6361 acaagccaaa cgttctttga caaccaaata aaggcaatcg aatacgtttc ggtaagaaaa
     6421 gagctgatga actaacacgc catgacctac aaaattagtg agcgattcat gttagtaatt
     6481 cgaaagtaat ctttagcagg attgctgcca tgactttctt atcgtgataa tgatactccc
     6541 gtatagccat agtccgagcg ttagaagcgt cacaggcaat gggtacggct acatgagaac
     6601 catgcagaag tgaaattcct gtgagctttg ctagagctgt tcgattgcta agatattctt
     6661 ctcttgtcag tgtattatta taaaactatt tgatataatt ataggtgaat attggagttt
     6721 tcctagccta taattgggaa aggagtaata atgataacgg tcacaaaaaa ggatttagtt
     6781 gagctaggat atggaacttc tttttcagca gacatcataa aaaaagcaaa agaattgatg
     6841 attacaaaag ggcatatcta ctatcaatct cgaaagctag atagagtccc tcgtgaagct
     6901 gttgaagaaa ttctaggaat tcggttttca aatgaaaaca ggtctgagta gtttttgcac
     6961 agtaaggagt aaaaactatg gttaaagacc caataaaaaa agcaaagaat ggaacctatt
     7021 attttcgtgc aaatctaggt tatgattcta aagggaaaaa aattcaaaaa tatcgtagtg
     7081 gattcactac taaaaaagaa gcaagagaag aatattcgaa actgctattg atgaaaccag
     7141 aagaattaag tgagaataag gataaaatga cgtttaaaca ttatatcttt gaaattttcc
     7201 ttccatggta taagacacag gtaaagctca gaacatatga aaatcgctta ccaactatta
     7261 agaagcattt ctcttatttt gataaaatgg ctgtttccga tattgagcct attgatgtgc
     7321 aaaattggca actaaaatta tcaaagaagt gtaagtcttc ttatgtaaga gctgttcaag
     7381 gactattttc agttgctatg gatcgagcaa ttgtccttgg gataaccacc acaaatccat
     7441 ctaagattat tggcaatgtg aaaaagcaaa agtctaaaat tgaattttgg acaaaagaag
     7501 aatttgaaaa agtaatctct ctaatttaca aagaagatta ctatcaacac tttttattta
     7561 tctctctgtg gtttttattt atgactggaa tgcgaattgg tgaagctact gccattcaat
     7621 gggaagatat tgattttgac tcaggagtac taaccatcaa caaaacactc tattacaaga
     7681 atcaatcgaa ttatagattt gttgaaccaa aaacaaaggc gagcgttcgt catattgtat
     7741 tagataaatg cacattaact tatttatccg aatggaagga agtacaacaa aatctaattc
     7801 agacagactt tgtaatgagc tataacggta ttcctaccca aaaacataca ctatcatatg
     7861 ctattgaacg ctatgctaag ctggctggta tccatcgaat cagaatccat gggttacggc
     7921 attctcatgc ttccttgtta atcagtatgg gagaaaatcc acttatcatt aaagatcgtc
     7981 taggacacga agatattgag acgactttag gaacgtatgg acatctttat cctaacagta
     8041 atttcgaggt agctaacaaa ttaaatggag ccatttcctt taaagaagca aaacaaaatt
     8101 atgatacttc accaaagaat caatttactg tagactactt gcgaaacaaa gaaatgaaaa
     8161 aagtgcaata aaagtgcagt aaaaagataa gaagagccag aaacccttgg tataagagga
     8221 tttctagctc tcctacaact attcccactc cactgttgct ggtggtttag aagtgatgtc
     8281 atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa
     8341 gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg
     8401 gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc
     8461 tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc
     8521 acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag
     8581 aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat
     8641 gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat
     8701 caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg
     8761 tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga
     8821 gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt
     8881 cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg
     8941 atctcttgca tcgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac
     9001 ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc
     9061 accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc
     9121 aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa
     9181 gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa
     9241 ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg
     9301 tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc
     9361 ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt
     9421 ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg
     9481 gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca
     9541 aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc
     9601 atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac
     9661 ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg
     9721 tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt
     9781 cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa
     9841 atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat
     9901 tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta
     9961 aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat
    10021 ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg
    10081 caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag
    10141 aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga
    10201 tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat
    10261 catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt
    10321 tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga
    10381 ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa
    10441 cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt
    10501 ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat
    10561 gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa agataaatca
    10621 gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac
    10681 ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc
    10741 gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg
    10801 ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga
    10861 gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact
    10921 tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga
    10981 catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc
    11041 ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga
    11101 aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga
    11161 tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt
    11221 tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca
    11281 aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga
    11341 agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa
    11401 aaaagttgta catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact
    11461 gacaattgtg atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac
    11521 atttggtaat gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa
    11581 agaataacta gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg
    11641 ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt
    11701 caaaaagaaa tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga
    11761 acttgtatag aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt
    11821 tccttttttc tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga
    11881 agggagccaa taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag
    11941 taacaactgt ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa
    12001 aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg
    12061 tgatcaatcg acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct
    12121 tctccaaact gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt
    12181 tatgcaattc tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc
    12241 gctcagtgga cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag
    12301 acagcggttt ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg
    12361 ataacggacg gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta
    12421 ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag
    12481 aagaacgttt caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga
    12541 aatttgttga acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac
    12601 tcaaacataa agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct
    12661 atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg
    12721 atggattaaa gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt
    12781 ttgatattgg attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa
    12841 aagttccgaa caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga
    12901 caaagggcta actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct
    12961 ttttttattt ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat
    13021 aataccgaat gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa
    13081 gagggatgga aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa
    13141 tggaaagatt ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat
    13201 gacaaatgaa gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga
    13261 tttgagttct cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac
    13321 gaccacattg attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc
    13381 tggacgcggt ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt
    13441 tcagatcgag ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt
    13501 aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt
    13561 cacagcgacc gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc
    13621 ggcaggagca gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa
    13681 catcgaagat gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg
    13741 agaaacagtg gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag
    13801 tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga
    13861 aatgtgctac gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga
    13921 tgaagcaaga acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa
    13981 agaaatgatc caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt
    14041 gactgcacgc tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt
    14101 ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga
    14161 agatattgat cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa
    14221 aggtgagtca ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt
    14281 attatataaa aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat
    14341 catcacagaa taaaggaaaa aagcggactt taccgtttta aagaaaaggg gaaaagaaat
    14401 ggaactaaac cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt
    14461 gatgcaaatt atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc
    14521 atgggttgcg ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat
    14581 cgggtttcct ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat
    14641 caataacggt gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga
    14701 tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct
    14761 tgtcaaagtt attattgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga
    14821 aatagcaaaa gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg
    14881 agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa
    14941 agcttccggc ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac
    15001 aagaatcgga acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta
    15061 attttttcaa aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt
    15121 gaagcgttag ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta
    15181 acggaatcag gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg
    15241 aattgtgccg agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg
    15301 catctagtcg tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa
    15361 gtaatggcag aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg
    15421 acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa
    15481 ctcatgttca ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta
    15541 cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat
    15601 ttttaggtgc atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag
    15661 acacaacaac tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc
    15721 agtcagcttg ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg
    15781 atggcttcca atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag
    15841 cattgaatgc tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc
    15901 aagaacaagc aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg
    15961 gattagataa cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg
    16021 ttgcagcagc ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg
    16081 aagtaatcga tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat
    16141 taggaaaaga aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag
    16201 gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag
    16261 gcggtacagg taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa
    16321 aagtttgggt tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg
    16381 gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag
    16441 atttggcaca aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat
    16501 tgaaagaaga cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag
    16561 ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag
    16621 aaaactaaaa ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat
    16681 tagaacaaga attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac
    16741 tccctgaagt aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat
    16801 cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc
    16861 atagaaatgc agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat
    16921 ttacaagtaa aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct
    16981 acattgatga acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt
    17041 gggaaagaag taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc
    17101 catcagcact ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc
    17161 gagcctagtc gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt
    17221 tctgaacagt atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga
    17281 atggaacaac gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt
    17341 gatgagccga cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag
    17401 gaattagtca aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa
    17461 gcagtagctg accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt
    17521 aaagacgtaa cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa
    17581 acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg
    17641 aaagaaaatc gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa
    17701 gtacttggta tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact
    17761 ggtttgcgaa aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa
    17821 aaaccccgaa aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac
    17881 gggttagtcc tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa
    17941 ccttacagta aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg
    18001 atcgaagaat atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc
    18061 ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt
    18121 gttgcaaatc caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg
    18181 atcgaacaaa gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt
    18241 ttaaatgttt cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat
    18301 ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa
    18361 gcacgaaaag agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg
    18421 taatttatta gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat
    18481 ggctgctttt ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg
    18541 ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt
    18601 aggtttttct gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact
    18661 ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt
    18721 tttgcttccg ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc
    18781 aggtttactt cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta
    18841 tatcttatta tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc
    18901 aagtttggac tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac
    18961 acaagctttt ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat
    19021 tttcgtttgg ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt
    19081 gaatcctttt gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat
    19141 ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca
    19201 aaacttcttt gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt
    19261 gcttggttct ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa
    19321 gatcggtgga cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc
    19381 gattgctttg attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa
    19441 agtcttccaa gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa
    19501 tcaaaaccat gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt
    19561 acttctacac tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct
    19621 gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt
    19681 tccatcgttt tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct
    19741 attttagtgg gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc
    19801 agtttgcgtg ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta
    19861 ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat
    19921 ctggggtatt tttctttccc aggattagca gatattccgg tcattggacc aattttcttt
    19981 aaaaatacat tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc
    20041 atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc
    20101 gacacattag gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg
    20161 ttaggcggta tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt
    20221 acgatcgttg gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct
    20281 ttaggggcaa tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc
    20341 gcacaattac cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg
    20401 ctgacaatca ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg
    20461 aagaactata tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt
    20521 gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa
    20581 tcatgtttaa acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag
    20641 atgccgataa gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag
    20701 gattgaacgt tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg
    20761 tggaatcagt gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga
    20821 aagatacgat gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg
    20881 tgtttcctaa tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta
    20941 aagtcgtatg taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc
    21001 aaatgaaaac aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg
    21061 cacatgaaga aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata
    21121 taacaaaaga tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac
    21181 caggagactt cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca
    21241 cagtccttga ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg
    21301 atattttcaa cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg
    21361 ttgaccagct gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag
    21421 tagattttga tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg
    21481 aagaatttga cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa
    21541 tgattacagc cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat
    21601 atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat
    21661 attattcaga tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg
    21721 gcgaaagctt cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa
    21781 gttaagcgag atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat
    21841 tgattttggt ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc
    21901 tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc
    21961 aggaaagcta gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt
    22021 ccactactat gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg
    22081 actaggggcc cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt
    22141 accaggagat ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat
    22201 cggtccgaat gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc
    22261 tgactatcaa gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg
    22321 cgtatatatg ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg
    22381 gacaatagga gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca
    22441 cagcggatta aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc
    22501 gaatctgagc catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc
    22561 attggttaaa gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata
    22621 ttgaagcgaa agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag
    22681 ctaaatatat tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta
    22741 tgttaggctt taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg
    22801 gaatgccatc agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat
    22861 tgattcgagt aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac
    22921 tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt
    22981 ttcctccaat cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg
    23041 gctatacggt tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga
    23101 cagagacgtt ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc
    23161 tttattactt aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc
    23221 atttgatcac cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga
    23281 tcgaagtagg attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc
    23341 attaagtaat aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact
    23401 tattattgca aatgtaagct tttgaacacc gtttaccaag aggttgtgaa tctgccgttt
    23461 aactctgagt cacaaggaac aatttcaaaa aacagctttt catgtttgat gaatattgtg
    23521 acttgtgacc gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg actgtgacaa
    23581 atcgtgtcac agtccctttt ttatagtgtt ttttataggt agtatttttt agcaactgtt
    23641 aagtcatcat ttaattcata aacaagtggt tgacctgttg gaatttcaag atccatgatg
    23701 tcttcgtcag agataccttc gatatgttta gctagggcgc gtaatgagtt accatgtgca
    23761 gctactaaaa cagttttgtt gtctagtaat gctggagcaa tttcatcttg ccagaatggc
    23821 aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag gaatatcgcg tttgtctaac
    23881 attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag tttcttccat taatggagga
    23941 agagtatcgt aagaacgacg ccagatgtgc acttgttcgt cgccgtattt ttcagctgtt
    24001 tcttttttgt tcaaaccttg taatttacca tagtgacgtt cgtttaagcg ccatgatttg
    24061 atttgtggta cccaaagttg atctgaatat tctaagatca agttacatgt tttgattgca
    24121 cgagttaaaa cagaagtgta agcaacatca aattcgattc cagcttcttt gattttgcgt
    24181 ccgccttctt ttgcttcttc gattccttct ggtgctagat ttacatcagc ccaaccagta
    24241 aattggttca atgcattcca ttcactaaga ccgtgacgag aaaaaactaa ttttggcatg
    24301 tttagctctc cttttaagat aggtttatat ttctgaaatt ttcagttctg ttttattgta
    24361 caattttttg gaagagattt ccatcgaaaa attagataag attctatgag aaaacaagta
    24421 gagaatataa aaaaacgaac agtgcccacg caactgttcg tttttaggta ttagatgcat
    24481 aattgctcga tttctttttc aggtaaattg atatatttat aacatgttcc aactgaaaca
    24541 ccgcattttg aagaaataaa ttggatcgtt tctttttttt cataatagag acgtcggatt
    24601 tttttgatcg tttttgcatc gatttttggt cttccgccga tttttccttt tttacgagct
    24661 tcatctaatc caactaatgt acgttctttg atcaatgcgc actccatgtt tgctaggttt
    24721 tccattaatt ggaaatagat ttttcccatt tcgcgcgtat cgattccttc atcaagacta
    24781 atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg ccgcgtagat
    24841 ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc cttgttgcat gtcagcgatc
    24901 actgactcca aatgaacagc agtagaatca ttttgttcca cttcatatgt ctcatggaaa
    24961 atttcttgac aaccaaagtc agataattga tcaacctgag ttgttaaatc aacgtcattt
    25021 atggttgtgc gtgcataacc aataattttc atggtgattc ctttctagca gtgattttct
    25081 aactggatat aacagtaaaa atgtagcata aagtacgtca aaaagatagt tttttcgcaa
    25141 attttccgct gtcttttttt attggatgat caagcagttt ttctttattt acgacggatt
    25201 gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta ctttagaaac catgtttagg
    25261 aaaagcccat gttctactac cccaacaagt tcatctaaat aggtagctaa gctttctggc
    25321 tgttcgatga cttctagatg cagatcaatg atgtaatgac caccatctgt ggttaagact
    25381 tctccgtttt cgtctgtacg caagacgggt tggaatcttt tttcttcaaa aatatgaagt
    25441 agctgttggg agccataagg gatcacttca actgggagtg ggaacttccc aagtttatcg
    25501 actaatttag aagaatcaac gatccaaatc gtttcttttg agtacgtagc gactattttc
    25561 tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa aatcagcact gatttcatca
    25621 gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa tactctttaa cggtattcct
    25681 aatttttccg cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat acgtaaacct
    25741 tcttcacgca tacggcgacc aatctcttct accatataat aggcggttga accggtcccg
    25801 agacctacga tcatcccgtc ctttacgaat ttcgcggctt caatgccagc taattgtttt
    25861 aaattcatct ttgcgtccac ctttttttag tttgagcatc gtgctttcct cactatttta
    25921 gcgtgattta cggtaaaaag ataccattct tttttattcc aataaagctt tttcaaaaaa
    25981 aattgcatct cttgttgaca tgttttttca ttcgtgatat tctaacaaag gtgctttatc
    26041 tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga taaggatgca
    26101 gacattgcat ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg aactagacgc
    26161 aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa ccaattagta cgtaaacctc
    26221 gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa caaaggatat aacagcttta
    26281 agaaaactca aactaacgta aactcacctc aaaaacgtgg ggtagcaact cgtgtgggta
    26341 caatgacacc taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt cgtttgtcaa
    26401 acttaattga agttactgct tatatcccag ggattggtca taacttacaa gaacacagtg
    26461 ttgtattatt acgcggtggt cgtgtaaaag acttaccagg ggtacgttac catatcgttc
    26521 gcggtgcttt agatactgct ggtgttaacg accgtaaaca aagccgttct aaatacggaa
    26581 ctaaaaaacc taaagcttaa ttttaattat taaacaaatg aaacttgctt ctactatata
    26641 gaagtaatta ctatgaacaa tatttcggaa ggaggagtta cggatgccac gtaaaggtcc
    26701 tgttgcaaaa cgtgatgttt taccagatcc tatttataac tcaaaattag taactcgctt
    26761 gatcaaccgt gtaatggttg acggaaaacg cgggattgca gctaacatta tctataatgc
    26821 ctttgacttg atcaaagaat ctacaggtaa cgatccatta gaagtgtttg aacaagcaat
    26881 gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt gttgggggtt caaactacca
    26941 agtaccagtt gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt gggtagtaaa
    27001 ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag aaatcatgga
    27061 tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa gacactcata aaatggctga
    27121 tgccaaccgt gcgtttgctc actatcgctg gtaagattct ctctgtttgg tcggctttta
    27181 taagtcgacc tcagatagtg tccttatttt tagcaactag atagtaaaga gaggagaaac
    27241 aaggagaatg accagagaat tttctttaca aaacactcgt aatatcggta tcatggccca
    27301 catcgatgct ggtaaaacga ctgctactga acgtatcttg tactatactg gtaaaatcca
    27361 taaaattggt gaaactcatg aaggagcttc acaaatggac tggatggaac aagaacaaga
    27421 acgtggtatt acgatcactt ctgctgctac tacagcacaa tggaagggtc accgaatcaa
    27481 catcatcgac acaccaggac acgtagactt cacagttgaa gttgaacgtt cattgcgtgt
    27541 acttgatggt gctgtaactg ttcttgatgc acaatcaggt gttgaacctc aaactgaaac
    27601 agtttggcgt caagcaacaa cttatggtgt acctcgtatc gtatttgcaa acaaaatgga
    27661 caagatcggt gctgacttct tatattcagt atcaacttta catgaccgtt tgcaagcaaa
    27721 cgctcaccca atccaattac caattggtgc ggaagacgac ttcacaggaa ttatcgacct
    27781 tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga acagagatcg aagaaacaga
    27841 aattcctgaa gaataccgcg aattagctga agaatggcgc gaaaaattgg ttgaagctgt
    27901 agctgaaact gatgaagaac ttactttgaa atacttagaa ggtgaagaaa tcactgaagc
    27961 tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta gaattctacc cagtactatg
    28021 tggttcagca tttaagaaca aaggggttca attactattg gacgctgttc ttgattactt
    28081 gccatcaccg ttagatatcc cagctatcaa agggattgac cctaaaactg atgaagaagt
    28141 agaacgtcct gcagacgaca gcgctccttt ctcagcttta gcatttaaag ttatgactga
    28201 cccattcgta ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac aatcaggttc
    28261 atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc tacagatgca
    28321 tgccaactct cgttcagaaa tctctgaagt ttacgctgga gatatcgctg ctgctgttgg
    28381 tttgaaagac acaactactg gggacacact atgtgatgaa aagagtctag ttattcttga
    28441 atccatggaa ttcccagaac cagttatcca agtcgctatt gaacctaaat caaaagctga
    28501 ccaagataaa atgggtgtgg ctttacaaaa actatctgaa gaagatccta ctttccgtgc
    28561 tgaaacaaac gtggaaacag gcgaaacaat catcgctggt atgggtgaac ttcacttaga
    28621 tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag gtgcgcctca
    28681 agtatcttac cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt ttgtacgtca
    28741 gtctggtggt aaaggtcaat acggtcacgt atggattgaa tttacaccaa atgaagaagg
    28801 tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg aatacattcc
    28861 agctgttgaa actggtttga aagatgctat ggaaaatggt gtcttagctg gctatccatt
    28921 agtagatatc aaagctaaac tttacgatgg ttcataccat gatgtcgatt caaatgaaac
    28981 tgccttccgt gttgctgctt ctatggcatt acgtgcagca gctaagaaag ctaatccagt
    29041 aatccttgaa ccaatcatgg ctgtagaagt tgttatccct gaagattatt taggtgatgt
    29101 tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt atggaagctc gcgctggcgg
    29161 ccaacaagtt gttcgtgcga tggttccact tgctgaaatg tttggttatg ctacaactct
    29221 tcgttctgct actcaaggtc gtggtacatt tacaatgaca tttgaccact acgaagatgt
    29281 accaaaatca gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg aataatttct
    29341 taaaaccgga tagtcctgca aagagattat ccaaagagac tatattttta atatagtttc
    29401 gtgtaaaata attatcgatg atatgggcat gtggtgtacc ttgtgtacat catataccta
    29461 tcagacaaat aaaaaatata ttgtattttt aggaggacat tttaaaatgg caaaagaaaa
    29521 atttgaccgt tctaaaccac acgttaacat tggtactatc ggacacgttg accatggtaa
    29581 aactacatta acagctgcaa tcacaactgt actatctaag aaaaacggcg gccaagctat
    29641 ggcttacgat caaatcgatg gtgctccaga agaacgcgaa cgcggaatca caatctcaac
    29701 agctcacgtt gagtatgaaa cagatacacg ccactacgct cacgttgact gcccaggaca
    29761 cgcggattac gttaaaaaca tgatcactgg tgctgctcaa atggacggag ctatcttggt
    29821 agtttctgct gctgacggcc caatgcctca aactcgtgaa cacatcctat tgtctcgtca
    29881 agttggtgtt ccttacatcg ttgtattctt gaacaaagta gacatggttg atgacgaaga
    29941 attactagaa ttagttgaaa tggaagttcg tgacctatta acagaatacg aattccctgg
    30001 tgacgatgtt cctgtagttg ctggatcagc tttgaaagct ctagaaggcg acgcttcata
    30061 cgaagaaaaa attcttgaat taatggctgc agttgacgaa tacatcccaa ctccagaacg
    30121 tgacaacgac aaaccattca tgatgccagt tgaagacgtg ttctcaatta ctggacgtgg
    30181 tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg aagttgaagt
    30241 tgttggtatt gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa tgttccgtaa
    30301 attgttagac tacgctgaag ctggagacaa cattggtgct ttactacgtg gtgttgcacg
    30361 tgaagacatc caacgtggac aagttttagc taaaccaggt acaatcacac ctcatacaaa
    30421 attctctgca gaagtatacg tgttgacaaa agaagaaggt ggacgtcata ctccattctt
    30481 cactaactac cgtccacaat tctacttccg tacaactgac gtaacaggtg ttgttgaatt
    30541 accagaagga actgaaatgg ttatgcctgg cgacaacgta acaatggaag ttgaattaat
    30601 ccacccaatc gctatcgaaa acggtactaa attctcaatc cgtgaaggcg gacgtacagt
    30661 tggtgccggt gtcgttacag aaatcaaagc ttaattcact taagttttaa agtaaagcaa
    30721 catttattcg gacgtaatcg ctcggaacga aggtcttagg atctttgttc cgagcttttt
    30781 tatttattgc aggatcagga aaaaagacag gagtattata aatagaagaa acacgaaata
    30841 aaagagtcag tattttctaa atgaaacttt atgaaaacaa aatgtttttt acaagaaaaa
    30901 gccttgcttt gtgagtttat atatattata atgacaaagg tgcgtttact ttagtgaatt
    30961 gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg agaggttgcg acacgcccgg
    31021 acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga gctatgtcta cttttcaaaa
    31081 taggcgaagg agggaacaag atggcaaaac aaaaaattcg tatccgttta aaagcgtatg
    31141 aacaccgtat tttagatcaa tcagcggata aaatcgtgga aactgcaaaa agaactggag
    31201 ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag tctttacaca atcatccgtg
    31261 cgactcataa atacaaagat tcacgcgaac aattcgaaat gcgtacacac aaacgtctta
    31321 tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt gatgaagcta gacttaccat
    31381 ctggtgtaaa catcgaaatc aaattataaa actaaaacaa aaataatgga ggtgtactca
    31441 tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca aatcttcact gaatctggtg
    31501 aattaattcc agtaactgtt gtagaagcta ctccaaacgt agtactacaa gtgaaaacaa
    31561 tggaaacgga tggctacgaa gctattcaag ttggttacca agatatgcgt gaagttttat
    31621 caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac ggctcctaag cgcttcattc
    31681 gtgaattcaa aaatgttgag ctaggagaat atgaagtagg aaaagaaatt acagtagatg
    31741 ttttccaagc aggagacatt attgatgtaa caggtactac gaaaggtaaa ggattccaag
    31801 gggttatcaa acgccacgga caaagccgcg gaccaatggc tcacggttct cgttaccatc
    31861 gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt atttaaaaac aaacgtcttg
    31921 ctggccgtat gggtggcaac cgcgtaacta tccaaaactt ggaagttgta cgtgtcgatg
    31981 cagaaagaaa tgttatcttg atcaaaggta atgttcctgg agctaaaaaa tctttaatta
    32041 ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga aaggaggaac taaggaatgc
    32101 cgaatgtagc attattcaaa caagatggaa gccaaaacgg tgaaatcaca cttaacgaag
    32161 aaatcttcgg aattgaacct aatgaaagtg ttgtttacga tgcaatcgta atgcaacgtg
    32221 cgtcattaag acaaggaaca cacgcggtta aaaaccgtag cgctgttcgc ggcggcggac
    32281 gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca aggatctatc cgctcaccac
    32341 aatggcgtgg aggtggcgta gtctttggac caacaccacg ttcttacagc tacaaacttc
    32401 ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc agaaaaagtt gctgaaaaca
    32461 acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa aacaaaagaa ttcaaacaag
    32521 ttcttgctaa cttatctatc gactctaaag tattggttgt tctagaaaaa ggtaacgact
    32581 ttgctgcttt atcagcacgt aacttaccaa acgtttctgt agtaacttct gataatgtga
    32641 gtgtgctaga cgttgtatca aatacaaaag tgttagcaac acaaactgct cttactcaaa
    32701 ttgaggaggt gcttgcataa tgaacttact agacgtaatc aaacgcccag tgatcactga
    32761 aaaatcaatg cttgccatgg atgacaagaa atacactttt gaagtggaca ctcgcgcaaa
    32821 caaaacttta gtaaaacaag cggtagaagc tgcttttgac gttaaagtca aaaacgtgaa
    32881 catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa tatgcaggat atacaaaaaa
    32941 acgtcgcaaa gcgatcgtta cattgactga agattcaaaa gaaatccaat tattcgaagc
    33001 tgctgaataa gcactaatag ataactcatt aaattaggag ggaaaaacgt ggcgattaaa
    33061 aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag gttctgattt tgctgaaatc
    33121 acaacatcaa cacctgaaaa atcattgtta caaccattga aaaaccatgc tggacgtaac
    33181 aacaacggcc gcatcactgt acgtcatcaa ggtggcggac acaaacgcca atatcgcgtg
    33241 atcgacttca aacgtaacaa agataacgtt gttgcaactg ttcaaacgat cgaatatgat
    33301 ccaaaccgtt cagctaacat cgcgttagta cactatgaag atggagtgaa agcttacatc
    33361 ttagcaccta aaggactaga agtgggtatg aaacttgttt caggtccaga agcagatatc
    33421 aaagtcggaa atgctcttcc attagaaaac attccagttg gtactgttat ccacaacatt
    33481 gaaatgaaac ctggtaaagg tggacaattg atccgttctg ctggtacaag tgctcaagta
    33541 cttggtaaag aaggcaaata cgtattgatc cgcttgaact ctggtgaagt tcgtatgatc
    33601 ttagctgcat gccgtgctac aatcggttct gtaggtaacg aacaacacga attaatcaac
    33661 atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc caactgtacg tggtagcgta
    33721 atgaacccta atgatcaccc acacggtggt ggtgaaggta aagctcctat cggacgtaaa
    33781 gcaccagttt ctccttgggg tcaaccagct cttggttaca aaactcgtaa caaaaaagct
    33841 aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat aattactttg accatgtgtc
    33901 gcacattttg agaggaggtt caccatgggt cgtagtttaa agaaaggacc ttttgtcgat
    33961 gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg aaaagaaaaa agttatcaaa
    34021 acttggtcac gccgttcaac agttttccca agttttgttg gatataccat cgcagtttat
    34081 gatggacgta aacatgtacc agtttacatc caagaagaca tggtaggaca caaattaggt
    34141 gaatttgcac ctacaagaac ttatcgtgga catgctgctg acgataagaa aacgaaacgc
    34201 taatttgagg ggaggataaa tccaaatggc agaacaaatc acatcagcta aagcaactgc
    34261 aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta attgacctta tcagaggtaa
    34321 aagtgtcgca gatgcaattt caatcttgaa attcacacca aacaaatctg ctggaatcat
    34381 tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat aactttgact tagatgttga
    34441 aaacttggta gtaagcgaag cttttgttaa cgaaggacct acaatgaaac gtttccgtcc
    34501 tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca agtcatatca cggtagtcgt
    34561 atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt acatccaatt ggaatgcgtg
    34621 taggcatcat ccgcgactgg gatgctaaat ggtatgctga aaaagagtat gctgagttct
    34681 tacacgaaga tttaagaatc cgtaaattta tttcaactaa acttgctgat gctgctgtat
    34741 caacagttga aatcgaacgc gcaacaggtc gcgtaaacat ttcaattcac acagctaaac
    34801 caggtatggt tattggtaaa ggcggttctg aagtcgaaag cctaagaaaa gaattaaaca
    34861 aattaactgg taaaagaatc catatcaata tcgtggaaat caaaaaacca gatctagatg
    34921 caaaattagt aggtgaagga atcgcacgtc aattagaaaa ccgtgttgct ttccgtcgcg
    34981 ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc tcaagggatc aaaactcaag
    35041 tatctggtcg tctaaacgga gcagatatcg ctcgttcaga aggatactca gaaggaactg
    35101 ttcctcttca tactttacgt gcggacattg attacgcatg ggaagaagca gatacaacat
    35161 acggaaaact aggagttaaa gtgtggatct atcgtggaga aattcttcca actaaaaaaa
    35221 acactgagaa aggagggaaa taatcatgtt agtacctaaa cgtgtaaaac accgtcgtga
    35281 attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa gaagtagcat tcggtgaata
    35341 cggtttgcaa gctgttgatt cacattggat cacaaaccgc caaatcgaag ctgctcgtat
    35401 cgcaatgact cgttacatga aacgtggtgg gaaagtatgg attaaaattt tccctcacaa
    35461 atcttatact gccaaagcaa ttggggtacg tatgggttct ggtaaagggg cacctgaagg
    35521 atgggttgca ccagtaaaac gtggtaaaat catgtttgaa atcgcaggcg ttcctgaaga
    35581 agtagctcgt gaagcgttac gtctagcttc tcacaaatta ccaatgaaaa ctaagatcgt
    35641 aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa agaaatcaga gaattaacca
    35701 ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga attgtttaat cttagattcc
    35761 aattagcaac aggtcaatta gaaaacactg cacgtattaa agaagtacgt aaatcgattg
    35821 cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt atggaaggag gccattaagc
    35881 gtatgactga aggaagaaat caacgcaaag tttatcaagg tcgcgtggtt tcagacaaaa
    35941 tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa ccaccctatc tatggcaaac
    36001 gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa caacacagca aaagttggcg
    36061 acatcgtgag aattatggaa actcgtccat tatcagctac aaaacgtttc cgtttgttgg
    36121 aagtagtcga agaagcagtt attatctaat aacacgagat tttcctatat agattgaatg
    36181 caaaatctga aaggaggata cacaacgtga tccaagcaga aagtcgttta aaagtcgctg
    36241 ataactcagg cgcacgtgaa attttgacga tcaaagtcct tggtggatct ggtcgtaaaa
    36301 ctgctaacat cggtgatgtg atcgttgcta cggttaaaca agcaacgcca ggtggggttg
    36361 tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac aaaatcagga gctcgtcgta
    36421 cagacggttc atatattaaa tttgatgaaa atgctgcagt gattattcgt gatgataaaa
    36481 gccctcgtgg aacacgtatc tttgggccag ttgcacgtga actacgtgaa aacaacttca
    36541 tgaagatcgt ttcactagca ccagaagttt tataatcttg agacacttat caaaggaggt
    36601 gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa agttatcact ggaaaagaca
    36661 aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa agataaagtt attgtcgaag
    36721 gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc tgctcctcaa ggtggaattg
    36781 ttgagatgga agcgccaatt catgtttcta acgtgatggt ggttgactct actggtgtag
    36841 ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt ccgtgtttct aaaaaaaccg
    36901 gtgaagtttt agataaataa aagtttaagg aaggagggtc ttactgaatg aaccgcctaa
    36961 aagaaaaata tcttaaagaa gtaactccat cattgatgga aaaatttaac tatagctctg
    37021 ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat gggtgttggt gatgcggtat
    37081 caaacgctaa aaacttagat aaagcagttg aagaattaac tttgatcact ggtcaaaaac
    37141 caatgattac aaaagctaaa aaatcaatcg ctggattccg tttacgtgaa ggaatgccaa
    37201 ttggtgcaaa agttacattg cgcggagaaa gaatgtacga attcttagac aaattggttt
    37261 ctgtttcact accacgtgta cgtgacttcc atggtgtaag taaaaaagcc tttgacggtc
    37321 gtggaaacta tactttaggt attaaagaac aattgatctt cccagaagta gattatgatt
    37381 tagtagataa agtacgtggt atggacatcg ttattgtaac aacagcaaac tctgatgaag
    37441 aatctcgtga gttgttgaca caattaggca tgccattcca aaaataatta aaggaggcga
    37501 actacgtggc taaaaaatca atgattgcta aaaacaaacg tcctgcaaaa ttttcaacac
    37561 aagcttatac tcgttgcgaa cgttgcggac gtccacattc agtttatcgt aagttccatc
    37621 tttgccgtat ttgcttccgc gaacttgcct ataaaggtca aattcccggc gtgaagaaag
    37681 ctagctggta aaaagtaaat tcgcataaga aggaggtaaa agatcaatgg tcatgacaga
    37741 tccaattgca gattttctaa ctcgtatccg taacgcaaac atggttaaac atgagtcttt
    37801 agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa atcttgaaac gtgaaggttt
    37861 cgttcgtgat gttgaataca tcgaagatga caaacaaggc gtgatccgtg ttttcctaaa
    37921 atttggtaaa aacggcgaac gtgttatcac aaacttaaaa cgtatctcta aaccaggttt
    37981 gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg aacggtttag gtatcgcaat
    38041 catttcgact tctgaaggtg ttgtcactga taaagaagct cgtgaaaaaa atatcggcgg
    38101 cgaagttatc gcttacgtat ggtaatcaag tagaatctac aaggaggtgt ctttaagtga
    38161 gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt aacagttact caagacggaa
    38221 acaacgttac agttaaagga ccaaaaggtg aattgactcg tactttttca gcagatatca
    38281 aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc aaacgacagt aaagaaatga
    38341 aaacaattca tggaacaact cgtgcgaact tcaacaacat ggttgtaggt gtcagcgaag
    38401 gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg tgcacaatta caaggatcaa
    38461 aacttgtatt gaacgttggt tactcacatc cagtagaaat cacaccacca gctggagtaa
    38521 ctgttgaagt accagctaac acacaagtaa ttgttaaagg tgcgaacaaa gaagaagttg
    38581 gcgaattagc tgcaaacatt cgtggaactc gtcctccaga accttataaa ggtaaaggta
    38641 ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa aactggtaaa taatcgttta
    38701 gaaaaattgc atagaagcca gcagcaggaa aaaggcattt ggctgaagtt taccttcaaa
    38761 aaaatacatt tttacttgtg ccgcagcttc tatttttcga aacgtattta ctgcttgaaa
    38821 tagtagtaca ataaaaataa agaggtgaca attgtgatta caaaaccaga taagaacaaa
    38881 acacgtcaaa agagacatcg tcgtgtacgt aacaatatct ctggtactgc tgagcgccca
    38941 cgcttgaacg tttttcgttc taacaaaaac atctacgctc aaattattga tgacgtagcg
    39001 ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa tttcaggtgg aacaaaaaca
    39061 gaagcagcac aagctgtcgg taaactagtt gctgaacgtg cagctgaaaa aggcattaaa
    39121 gtagtagtct ttgaccgtgg tggatacctt taccatggcc gtgtgcaagc tttagctgaa
    39181 gctgctcgcg aaaatggact agaattttag aagaaggagg aataccattc atggtttata
    39241 tcgatccaaa acatttggaa ttagaagacc gcgttgttgc aattaaccgt gtaacaaaag
    39301 ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt tgttgtcggt gacaaaaacg
    39361 gacacgtagg gtttggtaca ggtaaagctc aagaagttcc tgaagcaatc cgtaaagcaa
    39421 ttgaagatgc gaagaaaaac ttagttgaag tacctatggt tggttcaaca atcccacacg
    39481 aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa acctgctgta gaaggttctg
    39541 gggttgctgc tggtggacca gttcgtgccg tattagaatt agctggggta gcagatatca
    39601 cttctaaatc tttaggctca aatacaccaa tcaacgttgt tcgcgcaaca gttgaaggtt
    39661 tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg cggcaaatca gttgaagaat
    39721 taatcggata aggaggacaa caataatggc tgaattaaaa gttactttaa aacgcagtat
    39781 tatcggacgt cctcaaaacc aacgcgatac agttaaagcg ttaggtctta caaagattaa
    39841 cagttcagtg gttaagcctt ctaatgaagc aattaaaggc atgatcaata ctgtttctca
    39901 cctagtggac gttgaagaaa tttaatttag aaaaatcata actagaacat taaggaggtg
    39961 ccaaacctat gaaacttcat gaattaaaac ctgctgaagg ttcacgccaa gtacgtaacc
    40021 gtgttggtcg tggtacttca tctggtaacg gaaaaactgc cggacgcgga caaaaaggac
    40081 aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga agggggacaa acaccattgt
    40141 tccgtcgttt accaaaacga ggattcacaa acgtgaaccg taaagactat gcagtcatta
    40201 atcttgatgt attgaaccgc tttgaagacg gagctgaagt aactcctgtt gctttagttg
    40261 aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt attagctaat ggggaactaa
    40321 acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc agctcaagaa gcaattgaag
    40381 cggctggtgg atcaatcgag gtgatctaat gtttagacta ttaaaagatg cttttaaagt
    40441 aaaagacatt agatcgaaaa tcctatttac tgtatttgtg ttgtttgtat ttcgcctagg
    40501 tgcacatatt actgtaccag gcgtaaatgc tgcttcattg caaaaccttt cttctttgcc
    40561 atttttcaac atgttgaact tggtaagtgg tagtgcgatg caaaactttt ccattttctc
    40621 aatgggggtt tcaccataca ttactgcttc tatcgtcatt cagcttcttc aaatggacat
    40681 cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt ggtcgtaaaa aactgaatca
    40741 agcgactcga tatctgacat tagtattggc atttatccaa tctgtcggta tcactgctgg
    40801 attcaatcaa tacgctcaat taggtttcgt gaataatcct aacgtagcaa cttacgtaac
    40861 gatcggtttg attctaacag ccggtacgat gtttgttact tggctaggag aacagatcac
    40921 agaaaaaggt atcggaaatg gcgtatcaat gatcattttt gcaggtatca tttctagatt
    40981 gcctgaaagt atcagagaaa tttatgaaga ttactttata aatattgatc cttcagacat
    41041 atggaaatct gctatttttg tggccatttt actagtagcg gtcttgttga tcgtgacatt
    41101 cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc caatatacga aacgcgtagc
    41161 tggcgcgcct acaagcagct atctgccgtt gaaagtaaat gcagctggag ttattccagt
    41221 tatctttgct agttcgttta ttgctacgcc aaatgctgtt ttacaagcat tgagcggtag
    41281 attcggttct gaaaactggt acaaagtgat gatggagatc tttaactata atacagcacc
    41341 tggtgctacg atttatacga ttctgatcgt tgccttcact ttcttctacg cctttgttca
    41401 agtaaaccct gagaaattag cggaaaactt gcaaaagcaa ggaagttata taccaagcgt
    41461 acgccctggt aaggggactg aagagtatgt ctcaagatta ctgatgcgtt taagtacagt
    41521 tggtgcagtc ttccttggtt tagtggcatt gcttccaatc attgctcaaa tggtttggga
    41581 tcttccacaa tcaatcggat taggcggtac aagcttactg atcgttatcg gtgtagcttt
    41641 agaaactgcg aaacagttag aaggcttaat gctgaaacgt aaatatgttg gattcattga
    41701 gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct cacacattta ttaggaggga
    41761 aaacaatgaa cctcatttta atggggctgc caggcgcagg taaaggaaca caagctgaaa
    41821 aaatcattga tgcttatggt attccgcaca tctcaacagg agatatgttc cgcgcagcga
    41881 tgcaaaacga aacagctctt ggcctggaag caaagtctta tatggataaa ggtgcattag
    41941 ttcctgatga agtaacaaat ggaatcgtga aagagcgact agctgaacca gatacagaga
    42001 aaggcttctt attagatggc tttccacgta cgctagatca agcaaaagct ctggacgcaa
    42061 tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga tatccatgtt ggtgaagaaa
    42121 tcttggtcga acgtctagcc ggccgattta tctgcagcaa ttgtggagca acttaccata
    42181 aaattttcaa ccctacaaaa gttgaagaca catgtgatcg ttgcggtggg catgaattct
    42241 atcaaagaga agatgataaa cctgagacgg ttaaaaatcg tctggctatc aacatcaaaa
    42301 atagtgaacc aatcttggct tattataaag aacaaggttt gctaaacact attgatggtg
    42361 ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat cattgaaaaa tagcacgatt
    42421 gcccgtagcc gacttgcatt atgcagttac tcttgtcaag ttcaataaat tatgatagaa
    42481 tatgtcagtc cgacttctag agaatcctct agatgatcca ttttgagaat tttgaggtgg
    42541 gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa acaagtacaa ggaggtactg
    42601 tgcgtggcaa aagaagatat gattgaagtc gaaggtacag tcgtcgaaac tttgccgaat
    42661 gcaatgttta aagttgaact agaaaacgga caccaagttc ttgctactgt ttcaggtaaa
    42721 attcgtatgc attacatccg tatcttacct ggtgacaaag taacggttga attgtctcca
    42781 tacgatctta accgtggccg tattacttat cgctttaaat aattgtactc cgtaaaacta
    42841 caggaggtat tatcatgaaa gtaagaccat cagtaaaacc aatgtgcgaa cattgtaaag
    42901 taattcgtcg taaaggacgc gttatggtga tttgcccagc aaatccaaaa cataaacaac
    42961 gtcaaggata attggaggtg ctataaatat ggctcgtatt gcaggagtag atattcctcg
    43021 tgataaacgt gtagtagttt cacttactta tatctatggt attggtaaca ctactgctaa
    43081 aaagatctta gctgatgctg gtgtatctga agatgttcgc gttcgtgaat taacgaatga
    43141 acaaacagat gctatccgtg cagaagtaga taaattaaaa gttgaagggg atcttcgtcg
    43201 tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt tcttaccgtg gaatccgtca
    43261 tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac aatgcacgta ctcgtaaagg
    43321 cccagctcgc gctatcgctg gcaagaaaaa ataattctta agtgaaggag gttaaaactt
    43381 catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag aagaatatag aagcaggtat
    43441 tgcacatatc cattctacat tcaataacac tatcatcatg atcactgata ctcacggaaa
    43501 tgcattagcg tggtcatcag ctggttcatt aggtttcaaa ggaagcaaaa aatcaactcc
    43561 ttttgccgct caaatggcag cagaaactgc tacaaaagca gcaatggaac acggattaaa
    43621 gactgttgaa gtaacagtta aaggacctgg ttctggacgt gaagcagcaa ttcgttcatt
    43681 acaagctaca ggtttagaag tgactgcaat tcgtgacgtg actccagttc ctcataatgg
    43741 tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg ctcattttga gttttatcag
    43801 taacgtcatt gaacaagaca cttttcgttt tgaaaggggt aaagataaga atgattgagt
    43861 tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga ttatggcaag ttcatcgttg
    43921 aacctcttga aagagggtat gggactactt tagggaattc cctacgtcgt attttattat
    43981 cttctttgcc aggtgctgcc atcactaata ttcaaatcga tggtgtactg catgaattct
    44041 ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat cttgaacatt aaaggcttag
    44101 cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat cgatattacc ggacctgcta
    44161 cagtaactgc tggcgatatt atcgttgata gtgatgtaga aattcttaac aaagatatgt
    44221 acatctgtag tgtctctgaa ggagctacgt tccatgctcg cttaacagtg aaacctggac
    44281 gtggttatgt tcaagcagat gaaaataaaa aagaagatat gccaattggc gttcttccag
    44341 tcgattcaat ctacacacct gttcgtcgtg tgaactatca agttgagaac acacgtgttg
    44401 gacaccgtga agattttgat aaattgacga tggagatttg gacagatggt tcaattgaac
    44461 cgttggaagc aatgagtttg tctgcgaaaa tcatgactga acatttggat atttttgtga
    44521 acttaactga tgaagcaaaa aatgctgaaa tcatggtcga aaaagaagaa acacaaaaag
    44581 aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc tgttcgctct tacaattgtt
    44641 tgaaacgtgc aggaatcaat actgtacaag aacttacaaa taaatctgag cctgaaatga
    44701 tcaaagtccg taacttagga cgtaaatcat tagaagaagt taaagcaaaa ttgcacgact
    44761 taggtttagg tttacgtaaa gaagattaaa accttttacg aaggagggaa accaacgtga
    44821 gttatcgtaa actaggacgc acatctagcc aacgtaaagc gatgttgcgt gatttaacaa
    44881 ctgatttatt aatcaacgaa cgtattgtga ctactgaagc tcgtgcgaaa gaagttcgct
    44941 caactgcaga aaaaatgatc actttaggta aacgcggaga tttgcatgca cgtcgtcaag
    45001 ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga agaaaatgag gacatcgtca
    45061 ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc tcgttacgca gaacgtcaag
    45121 gcggatacac acgcattttg aagacagaac caagacgcgg tgatggtgca ccaatggttg
    45181 tcttagaact tgtctaaaat cacactaata acatcacttt atagatgatt cttttagagt
    45241 gttatgatgt tggaagatat ttttccgagt ctagctcagc gctgctcctc aagcgaatat
    45301 cgcttgggga gtaagcactc atcataaagt gtttttttat accttcaatt acctgtcatg
    45361 acagatatgt ttgaaggcat aaaaaaacat tttaagcgag aaataattga aaattttgtc
    45421 tttttttgat agtgttagta ggaactttag gatgaagaag gagtaatcat ggagccaatt
    45481 attgaattag aaaagataaa ttataaatac cagccagatg atcttcgccc cgcattgaaa
    45541 gatgtctcct ttacaattga taaaggtgag tggatcgcta ttattggaca caatggttca
    45601 ggaaaatcga cacttgccaa aacgatcaat ggattgcttc tgccagaatc agggatcgtg
    45661 aaagtgggaa atcaaatatt ggatgaagaa aatatttgga cgatccgaca gatggttgga
    45721 atgatttttc aaaatccaga caatcagttt gttggttcga cagtagaaga tgacgttgct
    45781 tttggtcttg aaaatcaagg gattcccaga gaagaaatgc tagtgcgtgt gaaagatgca
    45841 cttgagaaag ttagaatggc tgagtttgct tcacgcgagc ctgctcgttt atcaggaggg
    45901 caaaaacaac gtgttgccat tgccggcgtg gttgccttac gacctgatat cattatttta
    45961 gatgaagcaa ccagtatgct agatccagaa ggtcgtgaag aagtgatctc tacgatcaag
    46021 aagatcaaag aagaaagtca attgaccgtt atatctatca ctcatgatat tgatgaagca
    46081 gctaatgcca atcggatact agtgatgaga caaggcgaac ttgtccgtga aggtacacca
    46141 aaagaaattt tttctgctgg tcctgaactg atcgacttag gtctagatct accatttcct
    46201 gaaaaactga agagcgcact gaaagaacgt ggggttgatg tgcccagtga atatatgaca
    46261 gaagaaagga tggtggattg gttatggaca tccgttttga acaagtagac ttcacttatc
    46321 agccgaatac accatttgaa caaagagcct tatttgacat caatatgacg atcaaagaaa
    46381 acagttatac tgcattagta gggcataccg gaagcgggaa atcaacctta cttcagcatt
    46441 tgaatgcgct tgttaagcca acaagcggta cagtccatat tggagaacga gatattcagc
    46501 cggatacgga taataagaat ttaaagccca tccgaaaaaa agttggcatc gtctttcagt
    46561 ttccagaagc acagctattc gaagaaacgg tagcaaaaga cattgctttc ggtcctaaaa
    46621 actttggtgt cagcgaagaa gaagcactag tcctagcaaa agaaacatta gaacaagttg
    46681 ggctggatga aagctatttg gaacgttcgc catttgaact ttcaggaggg caaatgcgta
    46741 gagtagcaat cgctggtgtg cttgccatga ggccggaagt acttgtattg gatgaaccaa
    46801 cggcaggact tgatccccag ggcagaaaag aaatgatgga gatgttttgg cgtcttcata
    46861 aggaacagca gatcactatt gtattagtta cgcatttaat ggatgatgta gccaattttg
    46921 cagattatgt ctatgtgttg gaaaagggac gaatcgttaa tagcggggag ccgcaagagg
    46981 tctttcaaaa cattgaatgg ctaaaagaaa aacaactagg cgtacctact gccaccgagt
    47041 ttgcagaaga attaatggct aaagggatga actttgctac cttgcctttg acagctgaag
    47101 aattagcaga cgctatcgtc cattatgctg gaggaaatgc ccatgatgaa taaattgata
    47161 tttggtcgtt atatgcctgg agattccttg atccatcgat tggatccccg agctaaatta
    47221 ttagccagtt tttattttat cggaatcatt tttttagcaa ataattggga aacgtacctt
    47281 ttgttggcat ttttcacatt actttctgtc ttcttgtcaa aagtagattt gaaatttttt
    47341 atccgaggca ttcgcccatt gatctggcta atcctcttca ctgtagcttt gcaggtcttg
    47401 tttacaagtg gcggagaaac ttattggagt tggggtgttt tccatatcac tgaatttggt
    47461 gtgatgaacg gcctctttat tttctgtcga ttcgtgttga tcatttttat gtcgactcta
    47521 ctcacgctta ctacaccgcc tttggaatta tcggatgcga ttgagtactt gcttcgtcca
    47581 ttgaaagccg tacggttccc agtccacgaa atttcattga tgctgtcgat tgctcttcgt
    47641 tttgtgccta cattgatgga cgaaacagaa aagatcatga acgctcagcg cgctcgcggt
    47701 gttgattttg gtgaaggaaa cctagttcag aaaatgaaag cagtcattcc tttgctgatt
    47761 cctttatttg tcagcagctt caaccgtgcg gaagatcttg cgactgctat ggaagcaaga
    47821 ggctacaaag gtggggaagg aagaacgaaa tatcgtgtat tgcattggca taatagagac
    47881 acgatggtta tcattgcttt tgtattatta actgtgctac tgatcttttt aagaggataa
    47941 acgttcagtg ggccgctatc tattcttttc ttgctgatct gtttgcaaga aaggatgcaa
    48001 gcagtgtatg acaagaaata ttagggtctg tggcagacta aggtcacagg cctctttctt
    48061 ttatctgaaa agaaagaacg aaaaaatttt atagaaaaat cattattcca gataaccttt
    48121 taggaacaaa ggagagaaaa acatggtacg ttacaaagca atcattgcgt acgacggaac
    48181 aaattttaat ggattccaaa aacagccaaa tgggcgtact gttcaagaag aagtagaaaa
    48241 aacattacaa aaaatggcaa atggaaaaga aatcacggtc tttggttcgg gaaggacaga
    48301 tgccggagta catgcgatcg ggcaagtaat ccattttgat tatccagagg agcgtccgtt
    48361 agaacgaatg cgtttcgcgc tagataccca gtcaccagag gatattgctg taagacaagt
    48421 agaaattgtt tcagaagact tccacgcaag atatttggtc aaagaaaaga cgtatcaatt
    48481 tcgagtggat atagggaaac cacgcagtcc ttttcgacgc cattatgcaa gttattttcc
    48541 ttacccactt gatttggaaa aaattcagcg ggcgcttcct gatttattag ggactcacga
    48601 tttcacctct ttttgtgcat caggaagctc gatagaggat aaaatacgta cgatctacga
    48661 ggctaagatg gaggtaaatg aaacaaaaga cgaattgttg tttacattta ggggaaatgg
    48721 cttcttgtat aagatgatac ggatcttagt aggaacgctt ttgaaaattg gcaatggccg
    48781 attgcctgaa gatagtatcc cagatattat tgcgaaaaaa gatcgtaatg cagcaggacc
    48841 tacagcacac cctgaaggac tttatttgta tgaagtgaaa tatgaataag aggttatgat
    48901 agaagtggtc agcttcaaga aataagaagg tatttacgaa aattacttct caaaattttg
    48961 caaaatttcg gcttattttc gaagaagcta cttctggaac accgtttaag aaagaggtcg
    49021 tgagcctgtc atttaactct gagtcacaaa gagcaatttc aaaaaacagc ttctcatctt
    49081 tgatgaatat cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta
    49141 ttttttctgt aaccaaaaaa cgatggaacg ttgttttgat tgcccttggt gcggacagtc
    49201 tgaacaggtt ccatcgtttc tttattttct acgtcttctt ctgtgtgtgt ttcgtggttg
    49261 ttcttctatt gctggatcaa tcgtgccata aaaggggcga ttgcggtcaa ggtaccagac
    49321 ataaagaata aacaatgcaa tacataggac aaaaaggatt gcacctatcg taaatccttc
    49381 aggcaagtaa ctgaaagtga cagtatgttt acctgctgga acttttatca tcaagaatgc
    49441 gtctttgaaa ttgctgacct tgactggttt cccatcaatt ttagctttcc atcctttgtc
    49501 ataaggaata gttgtcacca acatggaatc ttcagcggta tttacagtcc cagaagcttt
    49561 tctgccagtg gcttccattt cgacaccttt ttctttgatc tgattcattg cggcttgata
    49621 ggctacaaca tctagaccaa cgactttagg ctccatgaaa cttatttctt tcgtcccata
    49681 aaaacttgca gtaaatgtca ctgttgtctc ttgaggataa tagccaagat catagtattg
    49741 accagaaatg ttgatttgcg tttttcgcga tgtaccattg acagtgatcg tcgcactaga
    49801 actttctaat tgcgcaaaat tggttgggaa caaacttaag taagcttgtg tatttgcagg
    49861 cacatggact tcccaagtga gctcttttgg ctggttatct gctgtttctg agtaagtgac
    49921 gccagtcgtt gtctttgtga tcgtcgtatt caactgattc ttcaaagtag gtgtatagaa
    49981 tgtaaaatac tgttcattta gaccagacaa tgtattgatc aagtttgtct gactggtcaa
    50041 attatctgtc actggctgct caacatcatt aacggacaac ggtgcagtaa atgctagtgg
    50101 caatgcattg acattctgat acagtgtata gtcgccgctt tgaccagctt ggcggaagcc
    50161 atatttactg aacgtgcttt cgtttttaga aatgttatat ttgattcctg taaacccatc
    50221 catcagcaaa gtattattgt tataacggat attcaaattt gtccctcttg aacggaaacc
    50281 taatttatcc aggtaagagg aagaattacg atttcttata gaagaaaaca aactgatacc
    50341 actgtaacca tagttgatcg aatcgtttgc agatactgga tccagatttt ccaagcggta
    50401 aaacgagtcg gaatcttttt ttgtttcatt tacaagagct ttcaagtcag gataaggatc
    50461 agtatataaa ctgcgggaag cataattcca atcatcaaga atgccatgga tcatactgtt
    50521 cgtattgacc agtgcttcag cagacatgat caataataac aatatactca gatgattgaa
    50581 tgaaagtttt ttatgctgat aaaatcccac agcaaaagca taaatcccta agaaaaccac
    50641 tgtcaataca aaggaagtca tcgtcacata ggtgtagctt ccttcaggtt tgatgcccca
    50701 tgccaccgca aagagagcag ctacgaccaa aatgattcct aacaacagcc caccatcgtc
    50761 ttgttctatc ttttcccatc cttttcctgc aagcaggagc actaagaaag agaacagata
    50821 ggcataacgg aataaaaaca tattcggtgc atgcatacca tgccagaaca aattcaatgg
    50881 aacaagataa aaacttgcga taagtatggc gaaaagactg ccgtacaaaa atttattttt
    50941 caacggtact ttccgactgg caaaataaaa cagacagagg actaatggta gcagacctgc
    51001 gtaaacgaac ggaatcgatc cgtattttgt cgtgtcataa acaccaatca tatttttcat
    51061 gattagatcc agataagaag tcgcttctgt tttcaacgta gtgatctgac tcagtgcctc
    51121 gccatttgac cgaagatcca gcactgctgg caatacaatg atcattgaag cgccgcctgc
    51181 cagtaaagaa gtgataccat aaggaacaat cgttttttta tattctgacc agtttgtcaa
    51241 tagacgggcg ataaaatata agaaagaaaa tacgccaatc ataaaaccca tataaaaact
    51301 ggataaaaac aagagtaaat aactgataaa taaaagtttc ggccgtttaa gatccataac
    51361 acgatgaatc cccaaaatca ctaatggaag ataaacaaat gcatccagcc acatgatgat
    51421 ctccgaatgt gcagtgataa atgagatcaa tgcgtaactg acgctaagtg caacatgccc
    51481 ccagcgagga atcttgaacg tacgtgcaag aaaccaaaaa gccaatccgg cacagccgac
    51541 cttaagcaaa gtcagaacat acagtgcatc tggcatcatc tggtttggaa aaaagaacac
    51601 cagtggcgtg aagatcccgc ctagatagta agaaattaaa gataaataat tcaatcctag
    51661 agaagcattc catgtataaa aaatactttg ctttccaaga agaacatttc ggaagctggc
    51721 atgaaaatta gaaaactgtg aaaaggcatc acttgccaaa atacttctgc tgcttccagg
    51781 atagattccg atacttagat aaacaagtgc taaaatcaaa aacggaatca ggaaactagc
    51841 tgctgcgtag cgtccgtttt ctctaaagaa tgactttaaa ttttttttca tatttatttt
    51901 ccaccaaatt tctatgaagt tgtattcagt ttatcatatc cttgtttttc tctgcttaaa
    51961 aagagtctag ttttcacaaa aaaataaaat ttagaataaa taacttcata atttgttcgt
    52021 ataacaaaaa taaacgcaaa aaaagggtag gaatcaaaag tcccaccctt aaaaattttt
    52081 attaaataaa aaaccgttca ctatctgaac ggtccaagta tcattcaatt aaacgcgttc
    52141 aacttttcct gatttcaaag cacgagttga cacccaaact tttttaggtt ttccgtcgac
    52201 cataacgcga actttttgta agttaggttt aacagtacgt tttgttgagt tcatagcgtg
    52261 tgaacggttg tttccacttt ttgtcttgcg acctgtaaag taacaaactt ttgccatggt
    52321 aatttcctcc tttgccttga tttggagcaa tagaataatt cagctcatac cagactaaca
    52381 tatcatatag aatgggaatt tgcaagtaca ttttcataga tacaattgac aattgagaag
    52441 aataattgta aattaaacaa ggttatacct gaaattttgc aaggtttttg agtactttgg
    52501 gtaactgcct tttatttcaa gttgcgctat gataaaatat gatagaagaa aaatgggagt
    52561 tttttacccg ttaaggaggc tcattcatgg ctgtaaaaat tcaaacgtca gctggaacca
    52621 ttgaaattac caatgatgta attgcaacag ttgttggtgg cgccgcgaca gatgtgttcg
    52681 gtgttgtagg aatggccagc aaaaatcaaa ttaaagataa tattactgag attttgcgta
    52741 aagaaaatta ctcaagagga gtagttgtcc gccaagaaga taatggtatt gctgtagatg
    52801 tttatacgat tatcagctat ggtacgaaaa tctctgaagt ttcccgcaac gtccaagaaa
    52861 aagtgaaata caatctagag gcattattag gcgtaactgc caattcagtt aatgtctttg
    52921 tacaaggtgt tcgtgtactg ccagactagg caggagactg gctggtaact cagcagtcga
    52981 tctgaaggag gattatctag gtgagtgtaa cagaaataag tgcaagccag ttccaggaaa
    53041 tggtccaggc aggtgcgaat cgtctgcaag taaatgcaga atacgtcaat tcattaaatg
    53101 tattccctgt gccagatggc gatacgggaa caaatatgaa tctatctatg actagtggag
    53161 ctaaggcagt agtagattca acctcagaaa aagtaggaga attagcagca atgctttcaa
    53221 aaggattgct gatgggtgcg cgcggaaatt ccggcgttat tttgtctcaa ttgtttcgtg
    53281 gattttctaa gcaaattcca gatgttgtct cattaaatgc ttctgatctt gctgcagcct
    53341 tcactcatgg agttgaaact gcgtataaag cagtaatgaa accagtagaa ggaacgattt
    53401 tgacagtagc tagagaagca gcgaaagctg gagaaaagaa agcaaaaagt actgatgatg
    53461 tcgttgaagt aatgacagct gttgtaaaag gagcaaagcg tgctttagct aaaactccag
    53521 acttgcttcc agtcctaaaa gaagtcggtg tcgtagacag tggagggcaa ggactgctgt
    53581 ttgtttatga gggcttttta agtgcattga atggagaatt ccaagcggat gaaacatatg
    53641 aaccttctcc agcagaaatg gatgatatgg tcaatgcaga acatcatcgc agtgtccaag
    53701 gtcaaatggc gacagaagat atccattttg gttactgtac agaaatcatg gtaaaaatcg
    53761 gagaaggacc tacagtcgat agtacattcg attacgaaga attccgtaac tatttagatg
    53821 gtataggaga ttctttactt gtggtcaatg atgacgagat catcaaagtc cacgtgcata
    53881 cagaaaaccc aggagaagtc ttgaattatg gacaaaaatt cggttcatta gtcaaaatca
    53941 aagtcgacaa catgcgcttg cagcatgaga cgattttgga acacgatgaa gaagtgaacg
    54001 agtttgcgaa aaaagaaata ccacgtatcc cgtatggtat cgtagcgatc gctgctggta
    54061 aaggcgtcca agagctgttt gaaagtttag gcgctaatta tgttatcagt ggtggacaaa
    54121 caatgaatcc aagtacggaa gacattgtta aagcgattca agaagtaaac gcagataaag
    54181 tgattgtctt gccaaacaat aagaacatct tcatggcagc tgatcaagca gcagaagtag
    54241 cagatatccc agtagcagtt gttccttcaa gaacgatttc ccaaggattg acagctatgc
    54301 tttcattcaa tgatcaagct tctttagaag aaaacaaaga aacgatgaca gaaatgttgt
    54361 ctagcgttgt gagcggtcaa gtcactcacg cgatcagaga tacctcaatt gacggtgtag
    54421 agatcaaaga aggagacttt ttgggcatga tcgatggaaa aatcctgatc tctaacccag
    54481 atatcacagc aacagcgtta agtacactag aacaaatgat caatgaggac acagagatcg
    54541 taacgatctt ggcaggtgat gaaggaacag ttgaacaagc aaatgaattt gctgagaaac
    54601 taacagaaaa acatcctgat ttggaaatcg agatccacca aggagatcag cctgtctatc
    54661 cttatctatt ttctgctgaa taatttctgt gtcagaatat ctttctaaca cagtattcat
    54721 tcataaaagg ccttcggtta gtgatcaggt atgatcgctg accaagtcct ttttttgtta
    54781 aaggagggag gaaaaattta tgcgttcttt agatgatcca atcactatgc tggcaggagt
    54841 cggagaaaaa agagcagcta gtcttgccag cttgggcatc caaacgattg aagacttact
    54901 gacttattat ccttttcgct acgaagatat tcaggaaaga aatctaaacg agatccagga
    54961 tcaagaaaag gttactttga aaggaatcgt agtctcgcct cctgtgatga atcgatttgg
    55021 ttataaaaaa agccgattgc aatttcgtat gatgcaagaa catgatgtgt tcaacgtttc
    55081 tttttttaat caaccttatc taaaagaaaa agtcatttta tcggaggaaa tcgccgtata
    55141 cggtaaatgg gatgcaaagc gtaaagcctt gaacgggatg aagattttag gttctcaaac
    55201 aatggatgac ttttcgccga tatatcacgt caacaaatca attcgtcaaa cgacattagt
    55261 tgatctaatc aaaaaagggt ttcaagaata cggcacattg attgaagaaa atctgccaaa
    55321 caaccttgtg gagaaatatc gactgcttga ccgagcttct gctgtaagaa gtatgcattt
    55381 tccaaaagac cacgaagaaa accatcaagc caaacgacgt gtcgtttttg aagagttctt
    55441 tttatttcaa atgcgtcttc aaggattgaa aaaagcagaa aaagcagaaa cgaatggttt
    55501 ggagatcctt tatgatgtcc aaaaattgaa acaatttact caaaagctac cttttgaact
    55561 gacaaatgca caaaaacgtg taacgaatga aatctgtcgg gatcttcgca gtccgcagca
    55621 tatgcagcga cttttgcagg gagatgtagg tagcggaaaa acagtggttg ccgcgattgc
    55681 gttatacgct accgttacag caggtttcca aggtgcgttg atggtgccta cagaaatctt
    55741 ggcacaacaa catatggaaa gcttgcaaca gctgtttgat cccaatgaag taaccatcgc
    55801 attgattacg gggtcaacga aagcaaaaga acgacgtgag ctccttgaaa aactagagca
    55861 aggggaaatt aacgtcgtga tcggcacaca tgcattgatc caagatggag tcgaatttca
    55921 gaatttaggt ctagttatta cggatgagca gcaccgtttt ggcgtgaacc agcggaaagt
    55981 acttcgtgaa aaaggctggc gaccagatgt cttatttatg acagcgactc cgattcctag
    56041 aacattagct attacagcat atggtgagat ggatgtatct atcattgatg aacttccagc
    56101 tggacgtatc ccgatcgaaa cacgctgggt aagaacacct caattggaca gtgtactgga
    56161 ttggacgaaa aaagaactgg caagaggaca tcaaatgtat gtcatctgtc cgctgattga
    56221 agaatcagaa gcattagatg taaaaaatgc agtggaaatc tatgaaaaac ttcgtgaact
    56281 atttgcacca aaatatgaag ttggcttgtt acatggtaaa atgaagaatc aggaaaaaga
    56341 agtaatcatg gaagccttca aagacaacca gatgcagata ttagtttcga ctactgtaat
    56401 tgaagtaggg gtaaacgtgc cgaacgcgac cgtcatgctg atcatggatg cggatcgatt
    56461 tggattggca cagctgcacc aacttcgtgg acgtgttgga cgcggaagtg atgcttccta
    56521 ctgtatctta gttgctaatc ctaaaaatga actaggtgtg gaacggatga aaatcatgac
    56581 agagaccacc aatggttttg tactaagcga aaaagatctg gaattacgtg gtcctggtga
    56641 agtatttggc ttccgtcaat ctggtctgcc tcaatttgcg attgctgact tggtgacgga
    56701 tggaaatgtt ttggaggtag ccagagaaga ggctgcagcc ctttggcaaa taaaggattg
    56761 gcagatgctt cctgagtatc agcctttagc gcaaagcctc caaatagaaa atcaggataa
    56821 tcgttatttc gattagcaga ctgtgctata ctttatactg atgacggaaa aaggagtgga
    56881 aacaatgaaa attgccgtag atgcaatggg cggagatcac gcacctcaag caattgtaga
    56941 aggcgtggct ttagcgcagc aagattttcc agaagtagaa ttcttacttt acggaaaaga
    57001 agcagaaatc aagaaatact taacaaatga aaaaaacatc acgattatcc atacagacga
    57061 aaaaatcaat agtgatgatg agccagtaaa agcaattcga cgaaaaaaga ctgcatcaat
    57121 ggtccttgct gcacaagcag tgaaaaacgg tgaagcagat gctgtatttt cagcagggaa
    57181 tacaggcgcg ctactagcag cagggttgtt catcgtagga cgtatcaaaa atatcgaacg
    57241 ccctggcttg atgtctacct tgccagtagt agggaaagaa ggtgccggtt tcgatatgct
    57301 ggatcttggc gctaacgcag aaaacaaacc agaacatttg ttgcaatacg gtattttagg
    57361 ttctttttat gcacgtaaag tccgaggtat cggacgtcct cgtgtcgcac tcttaaacaa
    57421 cggaacagaa gcaacaaaag gtagtgaagt gacgaaaaaa gcctatgaac tccttcagaa
    57481 tgaaacaagc ttgaatttta ttggaaacgt agaagctcgt gagctactca atggtgtggc
    57541 tgatgtagtg gtcacagatg gatttacagg caacgctgtt ttgaaatcta ttgaaggaac
    57601 agcaatgaat atgatgaatc tattaaaatc tgctattcta aatgaaggga tcaaaggaaa
    57661 aatgggcgct atgcttttaa aagacggctt acgttctttg aaagcagaaa tggattattc
    57721 caaacatggc ggtgccgttt tgtttggctt gaaagcacca gtcattaaaa ctcatggagc
    57781 aacaggcccc gatgctgttc gctatacgat ccgccaaatc cacacaatgc ttgagacaga
    57841 tgtagttgga caattagtcg aacaatttga aaaaagcgaa gaatagtatt tggatatctg
    57901 aagcgatccg ttccattgaa aaaaaggtgg acattcctga aaaatgccag taaaattagc
    57961 catggtgtag gtaattgaca agtgtatgat tcttggaggt gaaaaagttg actcgcgaag
    58021 aagtatttaa caaagtagcg aaaattattt caaatcattt tgagatagat accgataaag
    58081 tgacggatga attgaacatc aaagatgatt taaaagctga ttcaattagt attatggaat
    58141 tcgttttaga gcttgaagat gaattcggta cggaaatttc agatgaagac gctgaacaaa
    58201 ttgaaacagt cggtggcgca gtagattata tcagcagcca tcttaaataa tttcaaataa
    58261 aagcaaagaa ggataaaaag ctgcatgcag ctttttatcc ttcttttttt aatctttttg
    58321 acacgtaaaa gtcatttttc tgataattct ggcgataaac agaaattatg ttgcaaagta
    58381 aagcggtttt ctatataatt attaataatt cattacacaa agataagaat aacctttgtt
    58441 ataaaacgat ttattctgtg taatgtcctt ttttattaag aaaaacttcc atttaggaag
    58501 cagagagggg tattcgattg tccgataacc aattattaga agttcagaac ctacatacag
    58561 gattccgact caaagatgac tattacgatg cagtagatga cgtttcattt actttagaca
    58621 aaaacgagat tttagcaatc gttggggaat ctgggtgcgg gaaaagtacc ttggctacta
    58681 cgattatggg attacttgat cctaataaca caaaaattac aggtgagatt ttatataacg
    58741 atctgaattt aatcgacctg aacgaaacgc tctataataa aatccgcgga aatgatatcg
    58801 gaatgatttt ccaagatccg ttgtctgcat taaatcctct catgagaatc gaagaccaga
    58861 tcaaagaagg actttcctat cacacaaaga tgaatgcaga acagcgtcaa gctcgtgcac
    58921 tagaattgct agaacaagta gggattccta atcctgcgag agttggacgc caatatcccc
    58981 atgaactatc aggcggtatg agacaacgtg tcatcatcgc aatcgcaatc gcctgcaaac
    59041 ctccaatcgt cattgcagat gaaccgacga cagccctaga tgtaaccatc caagcacaga
    59101 ttcttgattt actgaaagac cttcaggaag aaacaaaaac agggatcatc ttgatcactc
    59161 atgacttagg agtcgtagca gaaatggctg atcgagtggc agtgatgtat ggcggacagt
    59221 tcgttgaagt agcaggtgta aaagaactgt ttgatcatcc taagcatcct tatacacgct
    59281 ctttactgaa ctctatacca caagaaggaa atcatgatgc agaactccat gtgatcgaag
    59341 gtgtcgttcc ttctctgaaa aacatgcctc gtaaaggttg tcggtttgca ccacgtattc
    59401 catggatacc agcaagcgat catgaagaag agcctatcct tcatgaagtc gcaccgaacc
    59461 acttggtacg ttgctcctgc tataagcatt tccactttag agacgaaaaa ggagaggtgt
    59521 agatgacaga attaatcaca atcaaagacc tgaaagttca ttaccctatt cgcagtggat
    59581 tcttcaatcg agtaacggat catgtatatg ctgtagatgg tgtggatttt attatcgaaa
    59641 aaggaaagac atatggttta gtgggagagt ctggctctgg aaaatctact acgggaaaag
    59701 ccgttgtggg attagaaaag gtaacagccg gacaaatcat gtatgaagga aaagatgtaa
    59761 cgaagcgaag caaccgcaaa aaaatgggtt ataacaagga tgtgcagatg atcttccaag
    59821 attcaatgtc cagtttgaat cctaaaaagc gagtgttgga tatcattgct gaacccatcc
    59881 gaaattttga acggttaagc gatcaagaag aaaagaaaaa ggtaaaagga ctgctagata
    59941 tcgttgggat gccggaagat gcgctatata aatatcctca tgaattttca ggcggacaac
    60001 gtcaacgtct aggagtagcc cgtgctgttg ctaccaatcc aaaactgatc gtagccgatg
    60061 aacctgtatc tgctctggat ctatctgttc aagcgcaagt attgaatttc atgaaacgaa
    60121 tccagcaaga atacggcctc agctatttat tcatttcaca tgatttaggt gtagtaaaac
    60181 acatgtgcga caatatcgca attatgtaca aaggtcgatt tgtagaaatc ggtacaagag
    60241 aagacattta taatgacccg cgacatatct atacgaaacg tctgctttca gctattccgc
    60301 aaatcgatgt agaaaatagg gaaagccaca aagaaaatcg acgaagagta gaacaagagt
    60361 atatccaaaa ccaaaaagaa tactatgatg cgactggtcg agtctacgat ttgcgtacga
    60421 tcacaactac acacaaagtg gcgttgaaag atggaggtgc aagctaatgt ggaaaacgat
    60481 tctgcgacgt gttttattga tgatcccgca agttattatt ttaagtgttc tgatcttctt
    60541 attagcccaa gcgatgccgg gggatccatt taccggtttg atcaatccaa atcaggatcc
    60601 ggcagtgatc gaacagatgc gacaagcagc tggcttgaat gacccatggt atgtccagta
    60661 cttccgttgg atcggaaatg ctttgcaagg cgattttggt caaagtttct tatacaaacg
    60721 ctctgttgct gacttgatcg gcgaacggat catcaacact ttatatctat cgatcttgac
    60781 agttgtcctt acttatttga ttgctgtgcc tctggggatg cttgctggcc ggtatcaaaa
    60841 ttcaatcttg gataagattg tcgtaatcta caacttcttt agttttgctg ttcctttatt
    60901 tatcttcggc ttgatcatgc tatttgtttt tggttatcgt ttaggctggt tcccaacaag
    60961 tggatcacaa acactaggtt tgcaaggtgg atttatcact caatggatag accgtttgaa
    61021 atatattctg ttaccatcaa ttacccaagc atttttggcg actgccgtca caatccaata
    61081 tctgcgaagt gaagtaatcg attcgaaatc gctcgatttt gtcagaacag cccgttccaa
    61141 aggggtaccg acgaataaag tattcagtcg acatattttc cgaaatgcag cgttgccgat
    61201 ggcttctcaa atggggtatg aaatcacttc gttgattgct ggttctgtag ttatcgaaaa
    61261 gatttttggt tatccgggag tcggaaaact atttattgat tccatcggcc aacgggacta
    61321 taccgtgatc acatccttag tattgatttt aggaatcgcg acgctagtgg ggacattact
    61381 ttcagatatc atcatgagta tcgttgaccc gcgtattcgg attcaataaa accaatcaga
    61441 aaggagaatg gatcgatgag tgaaaaaaac aatacagcag ttcaagaaag tattcctcct
    61501 atggggatac gaatgattgc tcgagaattt aaaaaagata aaattgctat tttttcatta
    61561 ggtttgctag tggttttatt attagtgatc tttatcggtg cgatgctgac cgatcaagac
    61621 aaagtgatgt atgttagtat cttcgacaaa tacgctgcac caggaacagt ttcgacacaa
    61681 ggacaaaagt tcattctggg agcagatgaa ggcggacgtg atgtattagg acaattgatc
    61741 ataggtgcta gaaactctgt tacgatcgga tttgcgatca cgatccttac ttcgatcatc
    61801 ggggttggat tagggatcat ctctggctac tacggaggga tcattgacaa catcttgatg
    61861 cggatcgttg attttatcat gattctaccg atcatgctga tcattatcgt cttcgtatcg
    61921 attatttcca gatacaatgt ctggtccttt atattcatca tgagtgcatt ttattgggta
    61981 gctaaagccc gattattccg aagcaagaca ttatctgaag caagaagaga ttatgtcagt
    62041 gcctcaaaaa caatgggaac caatgatttt aaaatcatgt tccgcgaaat catgccgaat
    62101 ctaagttcat taatcattac taatttgacg atgaactttg cggcaaacat cggtatcgag
    62161 acaactttga ctttcttagg tttcggcttg ccggcaaatg tacctagttt aggtacattg
    62221 atcggttatg caagtaacgg agaagtctta gtgaatcggc aatggatctg gttacccgca
    62281 tcaatcctta ttttagtttt gatgttgagt atcaattatg ttggacaagc attcaagcgt
    62341 tctgcagatg cgcgccaacg gttaggctag tatagaagcg aagggggaaa taggaatgaa
    62401 gaaaacgatt tttggatttg cagcgctgat gtctgctgtc gttcttgcag catgcggggg
    62461 cggaggtaca gatacctctg gaaacaactc tggaggcaac ggcaataaca aagaggaatt
    62521 aacttttcca attgcaacaa aaaataatga tgatgcaatc gaagacggcg aattagaagc
    62581 tgcggtcgca acggacagtc agtttaaagg attgttccag tgggagtttt accaagattc
    62641 atatgatgca gcttttatgg ctccttctca tgaaccgcta tttgcaaacg atgacgattt
    62701 cagaatcaca gacgacggag ctgcaacgct tgatctagat gaagatacga aaaaagcgac
    62761 aatcaaagta aaagacaacg tcaaatggtc agatggtgaa gatgtaacgg cggacgatgt
    62821 gattttccct tacgaaatca ttgggaataa agattacact ggtatccgtt atgacgacac
    62881 tttccgtaac atcgtaggta tggaagaata tcatgatgga agtgccgaca caatctccgg
    62941 aatcaaaaaa atagacgaca aaacagttga aatcgaatat aaagaagtaa acccaagcat
    63001 gcttcaatcg ggtggtggta tctggtcata tgcagcacca aaacatacgt tagaaggtgt
    63061 agcaatcaaa gatatggaat caagcgatca agtacgtaaa aatccagtga cttttggccc
    63121 ttattacatg agtaatatcg ttgcaggaga atcagtagaa tacttgccaa acgagcacta
    63181 ctggaatggt acgcctaaat tgaaaaagat cacgatgaaa tctgtaccga tggcaagtgc
    63241 aactgaagca ttgaactcta aattatatga tatgattttc caaatgccaa cagatactta
    63301 cgatacctat aaagaaattg ctggctatac aaacttaggc cgtgaacaaa cttcttatac
    63361 ttatctagga ttcaagttag gtaaatggaa tgcagataca aagagtgtgg aatacaatcc
    63421 agattcaaaa atggctgata aatcattacg tcaagcaatg ggctatgcat tagataataa
    63481 tgcagtaggt gaacgtttct atgcaggttt gagaagcaat gcgacttcat tgattcctcc
    63541 agtattccag tcataccatg atgatgagat gaaaggattc acacaagatc tagacaaagc
    63601 gaaaaaacta ttagatgatg ctggctacaa agatgtggat gacgatggtt taagagaaga
    63661 caaagatgga aataaactaa caatcaactt tgcttcgatg tccggtgggg aaacagcgca
    63721 accattggca gattactata tccaacaatg gaaagaaatt ggtttggatg tccaatacac
    63781 aacaggtcgt ctgatcgaat tcaattcttt ctatgataaa ttagaaaacg atgatcctga
    63841 aattgatgtt taccaagctg cttggaatac aggaactgat ccaaatccaa gtggactatg
    63901 gggaccaaat gctgcgttca actacactcg ttatgaatca gaagaaaata caaaattgat
    63961 caacgatatc agttcaagca aagcatttga tgaagagtac caaaaagaag catatcaaaa
    64021 atggcaacaa tatgcatttg aagaagcatt tgcgattcca acgttgttcc gtaatgaagt
    64081 attgccagtc aacgaccgtg tcaaagactg ggattggtct tatgatgtac caaacccatg
    64141 ggcaaatgtt tctgttacag cagattcacg ttcataatca ctaaaaatga ccgaaaaagg
    64201 gcgatgtcct ttttcggttt tttgatattt ttctgtttat ttctctaata ttcaggtcaa
    64261 ggcgctgatt tcatgacttc tccaataaac ggtgttcaaa gtccagtttc tcggtattaa
    64321 gtcaaattta tgcaaaaata tggaaagcca ttttcacaaa attttttctt catactcgaa
    64381 actaaacgaa tttttcacaa cttctcgtca atcactagga ttattaaagc tttacttacc
    64441 ttttgaccga accatttcaa atttgacaaa aaaccagtat aataaggaag tatcgaaaaa
    64501 atatccataa aaggaatgtg gaaaatggac aaccagctga caaaagaact aaaagaaaac
    64561 tataacatcg tttttcacga tctaaatttg cttgaacaag cttttaccca ttcatcctat
    64621 gtgaatgagc atcgcaatct gcaattatcg gataatgagc gacttgaatt tttaggagat
    64681 gccgttttag aactgatggt ttctcagtat ttattccgtt tatatcctca tttgccagaa
    64741 ggaaaattga caaaaacacg agctgcgatc gttcgcgaag acagcttgtc gaaatttgcc
    64801 aaagaatgtc actttgatca atatgttttg ttgggcaagg gagaagagaa ttcaggagga
    64861 cgtacgcgac cagcattgct ttgcgaccta ttcgaagctt ttttaggcgc gttatatctt
    64921 gaccagggat tcgacacgac ccatgcattc atcgaaaaag tgatttttcc taaagtcaaa
    64981 gcaggtgctt tttcacatga gatggatcat aaaacaaaac tacaagaagt cttgcaaaaa
    65041 tcaggtgatg tatcaatcga ataccgattg atcaatgaag aaggtccagc tcatgaacga
    65101 gtattttgga tcgaagttta tgtagatgat caattgattg gcactggaca agggaaatcg
    65161 aaaaaattag cagaacaagc tgcggcggaa aatgcattgg cagcactgta aaagtaagtg
    65221 agggaaagct ttgtatttga aacgaattga aatagccggt tttaaatcat ttgcagacaa
    65281 gacagtcatt gattttgaaa atagcgtcac agcagtggtt ggaccaaatg gcagcggaaa
    65341 aagcaatatt acagaggcta tccgctgggt attaggagaa caatctgcta aaagtctgcg
    65401 aggcggaaaa atgccggata tcatctttgc cggttccgat acccgtaagc agctgaatat
    65461 tgcagaagtc acagtgattt tagacaatag tgatcattat ctgccattgg agtacaatga
    65521 gatcagtgta acccgccgtt atcgacggac tggggaaagc gagtttttca tcaataaaca
    65581 aagctgcaga ttaaaagata tccaagaatt gttcttagat tctggattag gaaaagaatc
    65641 gttctcaatc atttctcaag gaaaagtaga agcgattttc agtagtaaac cggaagaccg
    65701 tcgtgggatc tttgaagaag cagctggtgt cttgaaatac aagcagcgga aaaagaaagc
    65761 agaacagaag ctttttgaaa cagaagacaa cctcagccgc gtccaagaca tcatccacga
    65821 attggaagaa cagttaactc ctttggccgc ccagagtgag gcagcaaagg agtttttacg
    65881 gctaaaagaa acactgactc aaaccgatgt ctctttgatg gttgctgaaa tcaagacagc
    65941 aaaaaaagat tgggacaaca aacaagcaca gcttgcgaaa ttcaatctag aattaggcaa
    66001 actatctgag tcgatccaag aacaagaatc cattttggcg aagcagcgta aagaaaatgc
    66061 gcaagccgat cgtctgatcg aaaaaaatca gcaagtatta ttagatcttt cggaaaaact
    66121 gaagcagaca gaaggacaaa aagatgtttt gcaggagcgg acaaagcata cacaaaaaag
    66181 ctcgcaagaa tatcaaactt cattagcaga agcacaaaaa aaggttaaac attttgaaaa
    66241 gcttcaagaa agcttgatga aagcagcagc agaaaaagaa acagagatcc aaaaagctga
    66301 agcaaatctc ataaaaacac agcaagaatt agaaaaatac caaaaatcaa cgaaagaatt
    66361 attggcagaa ttaagagatc aatatgttga tttgatgcaa gaacaagcag ctgtaggaaa
    66421 cgaactgaag tatctagaac gtcaatatat acaagaaaca gcaaaaagca agcagacgtt
    66481 agccaagcaa tcggaagtag aagcatcagt agaccgattg atcttacaaa aagaagaatt
    66541 gacacaaaag caggcgcagc taaaatcatc tttaactgaa acaaaagaga agttagaaat
    66601 gatccaacaa aacgggaaaa agttccaaga agcattagca aaagaacagc ctaaaatgta
    66661 tcaattaatg aatcaagtcc agcagctacg tgctcgtaag aaaagccttc aagaaatcca
    66721 agaaaactat tttggttttt accaaggggt tcgattggtc ttgcagcata aacagcagct
    66781 ttcgggtatt gtcggagcag tggctgaact gatcgatgtt cctgcagatt tcacgttagc
    66841 tatcgaaaca gctttaggtg gggcagccca gcatgtcatc gttgaaaatg aaaaagatgc
    66901 tcggcaagcc atcacgtatt tgaaacagca aagaggagga agagcaacat tccttccact
    66961 gacgacaatc aaaccaagac aattaccggc tcatatattg acacaagcag cagctgtaga
    67021 agggtttata gggattgcca gtgaacaagt cagttatccc gatcaaatcc aaactgtggt
    67081 tcaaaacctg ctaggaacga tccttttagc caaagactta actagtgcca atgcaatcgc
    67141 gcaaacgatc cgctatcaat atcgagtggt ttccttagaa ggtgatgtga tgaatgccgg
    67201 aggatccatg acaggtggag cgaataaacg aggaaaccaa ggcagtttgt ttgtacaaaa
    67261 tcaagaattg aaacagttga ctagtgaatt tgaagaagca gataaacaac tccaagcaca
    67321 agaaaaaaaa gttcaggagc tgcaacaaga aacagctcgt ttagccgaag aacaagaagt
    67381 tttgcgtaca cgaggcgaac aattacgttt tgaagaacaa gaagcgacaa atcagctaca
    67441 aaacatcata aatgaacttg aacgttttga aaaagaaaag caaatctcaa cttttgagac
    67501 acgtgaactg cagcagttta tcgaagatta cgaaaagcaa acgaacgaat tgaaagacaa
    67561 acaaacagat cttgaatcgc aacgacaaaa aatcgacgaa gaaatcaaat cgttaagtca
    67621 agaaagcgac cagatggaag ccagaagagc acaagtacaa agtcaaaaag cacaagaaca
    67681 agcagatctt gctgtcttga aagagcagtt taatcatctg cagatccagc tccgcggtgc
    67741 ccgagtccaa aaagcagagg caaccgaacg tcaagaagcg atagaaaagc agttggcgac
    67801 attgacagca gacttttctg atcatgaagt cacagaagaa agtttggaaa aacaaattaa
    67861 cgaattatct gctcagcgag aaacattaaa agccgagttg gcaaaagcca aagagcagcg
    67921 agatagaagg caaaaggaga tcgataagct tgaagcagtc ttggcagagc gtaatcgtga
    67981 gcaaaaagca cgactgtcgg aacaatccaa attagaagtc caaaaagacc gagctgaaat
    68041 gattttagat aatcatttga cctatctgca gtcagaatat cagctgacat ttgagaaagc
    68101 ttcccaagac tatcaagaaa caaccgatat cgaagacagt agaacaaagg tttcatcctt
    68161 aaaagaacag attgaaaaac tgggaccagt aaacttaaat tcaatcgaac aatacgaaca
    68221 agtcagtgaa agacatacat tcttagcaac acagcgagat gatctgttag cagcgaaaaa
    68281 ccaattgttt gaaacgatgg atgagatgga tgatgaagtg cggacaagat tcaaagaagt
    68341 atttgaagct atccgtcaag aattcaaaat tgtctttcca aatatgtttg gcggcggtaa
    68401 agctgaatta gtactgacgg aaccaactga tttgctgaaa acaggtattg agattgaagt
    68461 acaacctcct ggcaaaaaac tgcaaagtct aagtttgctt tctggaggag aacgagcact
    68521 tacagccatc gcgttgctct tttccattat ccgtgtctgt cctgttccgt tttgtatctt
    68581 agatgaggta gaagcagcgt tagatgaagc caacgtgaaa cgttttggtc gttacttgag
    68641 cgatttccaa gatgacacac aatttatcgt agtcacacac cgcaaaggaa cgatggtcgc
    68701 agcagatgta ctgtacggag tgacgatgca ggaatccggt gtatcaaaaa tcgtttctgt
    68761 acggatggaa gatatcaatg aagaggcgaa aatcaacgaa acgaaagtgt aggaacgaaa
    68821 atgatcaaat tagtagcaat cgatttggac ggtacattat taaatgaaca aaagctgatt
    68881 tcagatgaga ataaacaagc attagcacaa gcaaaacagc aaggggtcaa gatcgttcta
    68941 tgtacaggac gccccttagc tgcgatggcg cattatctgc aagaattagg actggtagat
    69001 gaaggtgact acagcatcac tttcaacggc ggtttggtac aaaaaaatga tactggtgaa
    69061 atcatggaaa agaaagtgat ggaagtatca gatatccaac gcttatacaa actggctcag
    69121 caattggatc tgccactaga tgttttgtct gacaatgtgg ttttacagct gcctagtgcc
    69181 ccaaggaagc aatccttgta caatgtgttg aataagctgc tgcaattcca accagcggat
    69241 ataagcgata ttacagatga atttgtgctt aataaggcag taatcgctta tgaccaagaa
    69301 gaattagatc ctaaaatcaa agaaatacca gcagaattcc atgaaatgta tgagatcatc
    69361 aaaacaagaa gtatgctttt ggaatttatg ccaaaaggtg ttacaaaagc ttatggtatt
    69421 tctttattgg caaaagatct tggtttgaaa caagaagaaa tcatggcgat cggtgatgag
    69481 gaaaatgatt taccgatgat ccaatatgct ggactaggcg tagcgatgga gaatgctgtg
    69541 cctttcgtca aagaagcagc agatttcgtg acagccagca atgaagaaaa tggcgtagcc
    69601 aaagtgatcc aaaaatttgt attagagtag ggggagtcaa atgggatttt ttgataaaat
    69661 caagcgtgct tttactggcg aagagaaaac agaagaagtt gaagtacagg agaagtacga
    69721 caagggttta gaaaagacgc gtaaaacatt tggtgaacgg atgaatgagt tgtttgccaa
    69781 ttttcgaaca gtcgatgaag acttttttga agaattagaa gaaacactta tcggtgcaga
    69841 tgtaggtttt gaaacagcga tacgaattac cgaagcatta cgtcaagaag tgaaactcag
    69901 aaatgcgaaa aaatcggcag aagtccaaaa tgcgatcatc gagaaacttg tggatctcta
    69961 tgaagcggaa ggaatcaatg aagtaaatga actaaatatc caagaagacg gactaacagt
    70021 tattttgttt gtcggtgtta atggtgtagg gaaaacaaca agtattggta aacttgccca
    70081 tcagttcaaa aacgaaggta aaaaagtatt gatggcagca gcagatacct tccgcgctgg
    70141 agcaatcgac cagttagttg tctggggcga acgcgctggt gttgaagttg ttcgtggcaa
    70201 tgcaggaggc gatccagcag ctgtcgtatt cgatgctttg gaaagagcaa agacagagca
    70261 ggcagatgtt ttactagttg atactgcagg ccgactgcaa aataaagtga acttgatgaa
    70321 cgaattagaa aaaataaaac gagtaatcca gcgggaatta ccagaagcac cccaagaaac
    70381 acttcttgta ttagatgcaa ccactggtca aaacgcgatg gttcaagcaa aacaattcaa
    70441 agaaacaaca gatgtgaccg gtcttgtttt gacaaaatta gatggtacag caaagggcgg
    70501 tatcgtactt gctatccgta atgaattgca tttaccagtg aaattggtcg gtcttggtga
    70561 aggaatcgat gacttggagc cgtttgatcc aaatgatttt gtcgttggat tgttcaaagg
    70621 attactaaaa gaagaataac ccatgtctaa aaagcaggag ctgtgacaaa agttgcagct
    70681 ccttgtttga atgattgttt ttaacatagc tgtgtttttg atgccttcat ttttatactt
    70741 atttgtaagc ggttctcaaa caatttcgtt ctttttctta gactttttga ttataattag
    70801 attcatatga attggaggtc acttaatgat gaattttaag caattattat tgcatgtcaa
    70861 tgcgcgccct tttatcgacc aagctcgatt tggtatagaa cgggaaggac agcgggttga
    70921 tcttgcagga aatctagcaa aaaccgatca tccagcaatc tttggcgatc gatcctatca
    70981 cccctatatc caaacagatt ttagcgaaac acaaacagag atgatcaccc ctgttaccga
    71041 ttctattccc gaattatttc agtatctcgc tgctgtttat gatgtgactg ctcgttctat
    71101 accgaaagaa gagatgatct ggccattaag tatgccacct gccttaccgg aaaaagacga
    71161 agagatcatt attgcaaaat taaaaaattt cgaagatgtc ttgtatcgac gttatttagc
    71221 aaaagaatac gggaagcgaa aacagatggt aagcggtatt catttcaatt ttgaatttgg
    71281 cgatgagttg ctaagaacat tgttcagcca tcaggaagaa tttcaagatt tttccgaatt
    71341 caaaacagaa ctttatttga aaacagcgag aaactttatg cgctatcgct ggatgatcac
    71401 ttatttattc ggtgcttcac caatgagtga gaagaactac tttttagatg aatcacatcc
    71461 gcaagaacct gttcgcagta ttcgcaacag tgcgctgggc tatacgaatc atccaaatgt
    71521 gaaagtttcc tatgcttcta tgaaacagta tttagcagat atcgagcgaa tgatcgaaga
    71581 agggaaactt tcggaggaga aagaatttta tacaccgctt cgtttccgag gcggaaagaa
    71641 agtcgcagat ttagcaacaa caggtgttcg ttatattgaa ttgcggaata tcgatttgaa
    71701 tccttatgca agattgggta tcaatccaga gcaagttcgg ttcttacaat tattcctgat
    71761 gtacatgtta tggacagaag aaaaagaaga ctgtgaccaa tgggtagcag aaggtacgac
    71821 tcgaaataac aaagtagcac tagaacaacc atcagatcaa acagaatttc atcaagaagg
    71881 tagagaaatt cttgaaggaa tgaagcaaat gctggttgaa ttggattggt tggattctct
    71941 ttatttagta gaggaagcat tgactcaaat ggatcatcct gaacaaacgt tagcagcgaa
    72001 actctatcaa gaagcacagc tatcgagtca gcaagaagtt gctgtggcat tgggacatca
    72061 atattataaa gaaagccatg aacgtcccta tcagttggcc ggttttcgcg aaatggaact
    72121 ttctactcag atatttatgt tcgatgccat ccaaaaaggt gtccaagtca aagtattaga
    72181 tgagtcggat caatttttgc gtctgcaatt ccaagaccat gtagaatatg tgaaaaatgc
    72241 aaacatgaca agtaaggaca gctatatcgt cccacttatc atggaaaaca aaacagtaac
    72301 gaaaaaagtc ttaaaagaag ctgggttccg ggtaccagga ggcgcagaat tttcatccat
    72361 ggaagaagcg gtaaaagctt atccgagatt tgccgagcag gcatttgtga tcaaacctaa
    72421 atcaaccaat tacgggttag gtatcacgat tttcaaggaa ggagctagtc tggaagatta
    72481 tcaagcaggt ttagccattg cttttcgtga agacagttcg gttttagtag aagaatttat
    72541 gccgggaacg gaatatcgtt tctttgtgat cgacggagaa gtccaagcca tcatgttgcg
    72601 agtccctgct aatgttattg gtgacagtat ccgaacagtg aaagaacttg ttgaagaaaa
    72661 aaatagtgac cctttgcgtg gaacgaatca tcgtgcacct ttagaattga tccaattagg
    72721 tgaattagaa caattgatgt tgaaagaaca agggttgacg atcgaatcag ttccccaagc
    72781 caatcaaatt gtttatctaa gggagaactc aaatatcagt acaggtggtg attcgatcga
    72841 tatgactgat gaattttctg aagcttacaa aaaaatcgcc gtttctgcgg tagaagcact
    72901 aggagctaag attagtggta tcgatttgat tataccagat aaagaaatcg atcctacaac
    72961 tgataaaaaa gcatatggga tcatcgaagc aaatttcaat ccagccatgc atatgcatgt
    73021 ttatcctttt gcaggaaaag gcagaagatt gacgatgaat gtattgaaat tgttatatcc
    73081 tgaagtattt taaaagaaaa agcttttgac agattgtcct atttgatgtc aatctgatca
    73141 aaagcttttt ttgcatgtaa aaatttatgt gttttttcgt tttaaaataa acgatcccag
    73201 aagctttttt tcttttttgg tttttctttt tcagctggcg aagagttttc ttctgggtat
    73261 tttttttcga tatcaatcac aggttcattt actgcttctt ttgctgttaa gagcaatccg
    73321 aattcctcag gatttgcagt gacgtaatcg ttgactacag tgaaacggct attgtttttc
    73381 gttgctagct ggatatattg gttttggaca gaaaggggta gagccccatt caagagtatg
    73441 gaagcatctg gatgtttttt gagttctttt aaaaagtttt ctttgttttt tggttggatc
    73501 atttcagcaa cagtcatact aagatagcag cgttccctga atgtccctaa atatttatgc
    73561 tgttcttcag ggttcactaa aggtgtacca taaagtccct tatccaaatg gctttttaca
    73621 tcatcttgtg ccataaaaaa taccctcctt cttctctttg tctttcttac gattatagca
    73681 tattcccaat aatacctaaa acatatgaaa aagcggttgc ttttgttcaa cgattcactt
    73741 aaaataagaa tgaaaatcat tctcaattag aaaggtggat tggatgcgct acggacgact
    73801 agtagcatta ttagttattt taagtagtat ttcattgttc atcggagtat cgaatgtaca
    73861 attgtcagaa ctgctccagt tagatgggca aagcttacaa atcttgctag tgactcgttt
    73921 gccacgaacg atttgtttga tccttgtggg agcgaccagt agtatttgtg ggttgatcat
    73981 gcaacatttg acccaaaata aatttgtttc accaacgact gcgggcacga tggatagtgc
    74041 gcgtttaggt attttagtgg ctatgatttt cctacctggt gcatccttga ttgtccgatc
    74101 cttgacagca ttttgctttg cttttgcagg aacattgctt tttttatccc ttacgcgatt
    74161 gttcctccag aaaaatcaat tgatgcttcc gttgattgga gtcatgttag ggaatatcat
    74221 tggttcagtt gctacttttt ttgcttatca attccagttg gtacaaaata tgacttcatg
    74281 gcttcaaggg aattttgcga ctgtgatacg aggaaattat gagttattgt atgctgttgt
    74341 tccattgttg ctcttggttt ggctatttgc ttaccgattt acgatcattg gtctgggcga
    74401 atcgtttgca gtaaatctgg gaatagatta ccagaagatg cagtttttag gcattggctt
    74461 agtatcattg gctagtgcag tcatgttgat catggtagga agcattcctt ttatcggagt
    74521 gatcattccg aatcttgtct ctagaatata tggagatcaa gtgcacaaaa caattggcgt
    74581 aactgcttta gcaggtagtt tgttcttagt attttgcgat cttgtcgctc gcgtgttgat
    74641 ttttccttat gaaataccag tcagtgtgat cgttggaatc atcggtgggg ctatcttcct
    74701 ttatcttctg ataaggggac gacgatatgc ttaaaaaaaa gccatctatt tttattggta
    74761 tgcttgttct tgttttattt gttacaggaa tgtacttgac gatccataca tatggaaact
    74821 ggcattttgc atgtgcattg aggagtaaga aaatttttgc atttatcctt gtctcgctag
    74881 ctgtcagcag tgccacaatc agctttcaga caattacgca taaccagttt ttgactcctt
    74941 cagtattggg acttgagtcg ttatatgtcc ttgtccaaac ggtccttttc ttctttatcg
    75001 gaggagtgac gatgcttcag cagacaggta cagttttttt cttattgaac gttgggctga
    75061 tggtcgggct gagtctattg ttatttttct ttcttttgaa aaaaatgaga gcgaatcttt
    75121 ttttattact gatgtcaggg atgattttag gtacgctttt tggtagtgtc agtacctttt
    75181 tacaagtcat catggatccg aatgaatatg atttgcttca aggcagatta tttgcaagct
    75241 ttggcaatat ccagagtcag tatcttttgc ctgcttcttt gatcattatc ggctgctggt
    75301 acctgttatt tcgatatcga catgtgctag acgttttcca tctcggaaat gaacaagcag
    75361 taaacctcgg gatagacaca gatcgttttg aatttttgat tttatgttgt gtcagtcttt
    75421 tgacagggac atcaacggct cttgtgggtc cagttacttt tttagggttt attgtcgcaa
    75481 atatcagtta tcgtgtcttt tctacttatc gtcatggtca gctatttatc ggaagctttc
    75541 tggtagctat gctgtttctg acaggaggac agctcgcagt agagcagatt ttcggctgga
    75601 atacaacagt gagtgtcgtc gttgaatttg tcggaggaat ttattttgtc acaaaattga
    75661 ttcgcgaaag gaagagtatc ctatgattga tttacaaaag gtcaataaac tgtaccatgg
    75721 aaaatcagtg gttaaagatg tacagttaac gattcctgaa aagaaattga ctgcttttat
    75781 cggtccaaat ggtgctggaa agtcaacggt gttgtcgatg atcagtcgtt tgataccaaa
    75841 agatacaggt gaaatctatt tagaccataa cgaagtaaag gcttggaaat ccaatgaatt
    75901 agccaaaaaa ttatcgatcc tacgacaatc gaatgaaata aatgtccaaa tcaccgtgaa
    75961 agaattagta gaatttggac gatttcctta tacgaaagga aaattgacga caaaagacga
    76021 ggaaatcgtt caggaatcac tggaacacct tgggttagaa gaaatagcgg atcaatcgat
    76081 tgacactctt tcaggcggtc aactccaacg tgcgtatatt gcaatggtgc tagcgcaaga
    76141 cacggattat attttactgg atgaaccttt gaacaatcta gatatgaatt ttgcggttca
    76201 aatcatgcag attttaaaaa atttagtaca agaaatcggg aaaacaattg ttattgttct
    76261 tcatgatatc aactttgctg caagttatgc agacgaaatt gttgcaatga aagatggaca
    76321 gttatttacg caaggaacga cccaagaaat gataaaaaaa gatgtattgg atgaattata
    76381 cgaaatggac atacggattt gtgagattga aggccgcaga ttctgtcttt atttctaaaa
    76441 ctaacattag gagagaagag atgaaaaaat tagtagtagg tatcttgatt ctagcatcat
    76501 ttggtttagc agcatgtgga aatagcaaca acaccagtca ggcagacaca aaaaaaagca
    76561 gcacacaaac tgaaactaca ttgacgataa cagatagtaa tggagaccaa atcgaggtgc
    76621 caaataatcc agaaaaagtc gtcgtattcg acaatggttc tttagatacg atggatgctc
    76681 ttggtgttgg agataaggta gttggagcag ctacaagcag ccttcctgag tatctttcat
    76741 cctataaaaa agtagaatca gcaggtggaa taaaagaacc agacttagaa aaaattaatc
    76801 agcttcagcc agatctaatc atcatttctg gccgacaaag agattttcaa aaagatctaa
    76861 gtcaaattgc tccgaccatt ttcctttctt tagatgcgaa aaatccatgg gaatctttcc
    76921 agcaaaatgt cactgcttta ggggagatct ttggtaagca agaagaagct aaaactcaat
    76981 tagaggaatt atctagtgca atcgatcaga cgaaaaagaa agcagaagct actgacaaaa
    77041 aagcactagt cacacttgtg aatgaagggc aattatcagc ctacggatct ggttctagat
    77101 tcggtttcat ccatgattta tttggctttg aacaagcaga tgatcaaatc gaagcatcca
    77161 ctcacggtca aagtgtttct tatgaatatg tgctggaaaa aaatccggat atcttatttg
    77221 tagtagacag aacaaaagct attggtggag acgacagcaa agatgatatt tctgccaatg
    77281 aactggtagc acaaacgaat gctggtaaaa atcagcagat catctccctt gaaccagatg
    77341 tctggtattt aagcgggggc ggtttagaat caatgaaact gatgatcgaa gatgtgaatc
    77401 aagcatttaa atagcataaa taacagagga ttaaactctc atactatgta acaataatgt
    77461 ttcttacatt cttaaatagc aagagcctat cctcaaagaa gttatcaaaa accatccgta
    77521 aaatcgtatt gatgatttta cggatggttt ttatttattt gagaaatctt ttttcaacgt
    77581 gaattcatta tctacatcat acgggttgat tctagaatgg ggagtggcaa aaaatagtgt
    77641 gatccatttt ttcagcatca agatcgcctc ctttgatttg aatcaaaggt acaaagcgaa
    77701 acttagataa aacttaaatt tagatatttt tattttgtac cgatctcttc tttaaacgat
    77761 gttccatctg ctgcttctcg gcaataagtc cactagacta gaaaatatga gaagctattt
    77821 tctgtccagc gttcttatta ctcaaagcaa atcgcagttg tcacaatctc ttctaaacga
    77881 tgttccatct gctgcttctc ggcaataagc ccactagact agaaaatatg agaagctatt
    77941 ttctgtccag cgttcttatt actcaaagca aatcgcagtt gtcacaatct cttctaaaca
    78001 atgttcaatc agctgacctt cggtattagc tcaaagcact gatttcacaa cctctattta
    78061 ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc atttttcgat aaaaatcggc
    78121 gggaagctta ctccaccgtt ttaataattg atagaaataa ctctcttctg ctttgatttt
    78181 atactcgctt aatatcaatc caaaactatt cgtttttttt gaagctaatt ttttataaat
    78241 atccaatgct ttggttttat cttcattgac aaagagtgtt tttacttcaa tataagaacc
    78301 ctcgatattt tgtgtttctt cgataaaata gatgtttttt atatattcct tcatagtgat
    78361 tcctccaaag aagtctttta tttgataagt ttactctacc gaagagttgt taataatccg
    78421 tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt tttttatttt ttatagtatt
    78481 ttttgttatt taagaaagga ataaggttaa acaaataaca ggttgctttt tgggtctttt
    78541 tccgttcaag aaactgaacg aagaaaaggg aaagttgtct cttttgtgat aagagaaatc
    78601 ataataaacg aatgacttac tttccaataa attgagaaca gtcagaacca gacaaattag
    78661 ttgcatctga tgaataaagt gattattcta tgacatgagg tgatacagat gaaattagaa
    78721 gtgacaaaag aagcacaaga agtattgaag aataaattag aagcagacga tcaactttta
    78781 ttagatatcg aaaacggtga cggtccattt gcagaaagcc aagtcagttg ccaattggat
    78841 acggcatttc gattgatcat tgtcaaaaaa gatacacaaa cagatctaag tctttattct
    78901 gtagtggctg atacaccagt tggaccaatc aaaatcaaag acagtgcaat actttacatg
    78961 gatgatccat ccacacttgc tttagaacca acttataata gtttacaatt aaaaggacca
    79021 agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga taaaatggga
    79081 aaattggcga tcatcagcga cctacatgtg gacatcaata aattcggcga atcagagctg
    79141 atgcagcttt ggcaagtttt gcaaaagcac aagatcactc ggcttcatct agccggagat
    79201 acggctaata aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa taaaggatta
    79261 tctacgacat ttcatttcgg caatcacgag ttggcagatg tgcccaatga acagatgatg
    79321 gaccattttc cagacccaca ttttttaaat gaacggtata ttgctttaaa tgagcagacc
    79381 gttcttttag ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa agattacgag
    79441 gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt tcatccagcg tgggaaaaca
    79501 gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga ccagctggaa
    79561 gcaaaaaaac ttgaggtgat tttatccact catttcgttc ctaaacgggc atttatcatg
    79621 tatcaatctg ctccttatga gcgttggaac aaattaaatg catttttagg atcagaatca
    79681 ttcggagaat tacttgaccg ttattcaaat atcaaacaag tagtttttgg ccatacgcat
    79741 cgtcgctttg aagataaatc catcggtcac acgatttaca gctgcagacc ttttggttat
    79801 tattatgaat ggcagctgac ccgagatttt gtcttgaaag aacagttgct agacagctat
    79861 gatcctatga aattacgtgt cctcttgcgt gcccatcacc cagcttttca ggcctatcaa
    79921 acacaacatt tgcaagagga atttgaacaa gcgatgacaa tcgttccgta ttgagacagc
    79981 attagtaatg tgtaaaagaa aataccttgt aaaacataag gataagtaag agcagtagat
    80041 ccatttatcg tggatatgct gctttttttg cggaaaataa tcagcgaatg tttatttgat
    80101 cggatgattt gtaactcgca gagtactaga tgcaagcgtg tattcttttt ttgaacgata
    80161 atcacaaaaa aaaatata
//
LOCUS       DAZKBH010000004        61153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000004 DAZKBH010000000
VERSION     DAZKBH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.004"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG8OO_000281"
     CDS             352..1041
                     /locus_tag="ACG8OO_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9044220.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG8OO_000282"
     CDS             1028..2236
                     /locus_tag="ACG8OO_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9044221.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG8OO_000283"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG8OO_000283"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9044222.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8OO_000284"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8OO_000284"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9044223.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG8OO_000285"
     CDS             complement(6339..7034)
                     /locus_tag="ACG8OO_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFP9044224.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8OO_000286"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8OO_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9044225.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG8OO_000287"
     CDS             complement(8233..8424)
                     /locus_tag="ACG8OO_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFP9044226.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG8OO_000288"
     CDS             complement(8445..9335)
                     /locus_tag="ACG8OO_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP9044227.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG8OO_000289"
     CDS             complement(9322..10089)
                     /locus_tag="ACG8OO_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9044228.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8OO_000290"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8OO_000290"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9044229.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8OO_000291"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8OO_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9044230.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8OO_000292"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8OO_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9044231.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG8OO_000293"
     CDS             14660..15043
                     /locus_tag="ACG8OO_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044232.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8OO_000294"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8OO_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFP9044233.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG8OO_000295"
     CDS             complement(15987..16805)
                     /locus_tag="ACG8OO_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFP9044234.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8OO_000296"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8OO_000296"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9044235.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8OO_000297"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8OO_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9044236.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8OO_000298"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8OO_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9044237.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8OO_000299"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8OO_000299"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9044238.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8OO_000300"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8OO_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFP9044239.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACG8OO_000301"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACG8OO_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9044240.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8OO_000302"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8OO_000302"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9044241.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8OO_000303"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8OO_000303"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFP9044242.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8OO_000304"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8OO_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9044243.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8OO_000305"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8OO_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9044244.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8OO_000306"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8OO_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9044245.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30087
                     /locus_tag="ACG8OO_000307"
                     /pseudo
     CDS             28113..30087
                     /locus_tag="ACG8OO_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
     gene            30093..30545
                     /gene="rplI"
                     /locus_tag="ACG8OO_000308"
     CDS             30093..30545
                     /gene="rplI"
                     /locus_tag="ACG8OO_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9044246.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30819..32186
                     /gene="dnaB"
                     /locus_tag="ACG8OO_000309"
     CDS             30819..32186
                     /gene="dnaB"
                     /locus_tag="ACG8OO_000309"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9044247.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32966..34258
                     /locus_tag="ACG8OO_000310"
     CDS             32966..34258
                     /locus_tag="ACG8OO_000310"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9044248.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            34994..35857
                     /locus_tag="ACG8OO_000311"
     CDS             34994..35857
                     /locus_tag="ACG8OO_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9044249.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(35986..37608)
                     /locus_tag="ACG8OO_000312"
     CDS             complement(35986..37608)
                     /locus_tag="ACG8OO_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9044250.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            37857..38378
                     /locus_tag="ACG8OO_000313"
     CDS             37857..38378
                     /locus_tag="ACG8OO_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9044251.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38564..40294)
                     /gene="spxB"
                     /locus_tag="ACG8OO_000314"
     CDS             complement(38564..40294)
                     /gene="spxB"
                     /locus_tag="ACG8OO_000314"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFP9044252.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40459..41277
                     /locus_tag="ACG8OO_000315"
     CDS             40459..41277
                     /locus_tag="ACG8OO_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFP9044253.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41380..41577)
                     /locus_tag="ACG8OO_000316"
     CDS             complement(41380..41577)
                     /locus_tag="ACG8OO_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044254.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41746..42324
                     /locus_tag="ACG8OO_000317"
     CDS             41746..42324
                     /locus_tag="ACG8OO_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9044255.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42419..42556
                     /locus_tag="ACG8OO_000318"
     CDS             42419..42556
                     /locus_tag="ACG8OO_000318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044256.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43369..43872)
                     /locus_tag="ACG8OO_000319"
     CDS             complement(43369..43872)
                     /locus_tag="ACG8OO_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9044257.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44231..45256
                     /locus_tag="ACG8OO_000320"
     CDS             44231..45256
                     /locus_tag="ACG8OO_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9044258.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45579..46457
                     /locus_tag="ACG8OO_000321"
     CDS             45579..46457
                     /locus_tag="ACG8OO_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFP9044259.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            46933..47049
                     /locus_tag="ACG8OO_000322"
     CDS             46933..47049
                     /locus_tag="ACG8OO_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP9044260.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            47977..48315
                     /locus_tag="ACG8OO_000323"
     CDS             47977..48315
                     /locus_tag="ACG8OO_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044261.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48409..48639
                     /locus_tag="ACG8OO_000324"
     CDS             48409..48639
                     /locus_tag="ACG8OO_000324"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFP9044262.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49039..49410
                     /locus_tag="ACG8OO_000325"
     CDS             49039..49410
                     /locus_tag="ACG8OO_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFP9044263.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49684..50376)
                     /locus_tag="ACG8OO_000326"
     CDS             complement(49684..50376)
                     /locus_tag="ACG8OO_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9044264.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50379..51164)
                     /locus_tag="ACG8OO_000327"
     CDS             complement(50379..51164)
                     /locus_tag="ACG8OO_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9044265.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51401..52801
                     /gene="lacG"
                     /locus_tag="ACG8OO_000328"
     CDS             51401..52801
                     /gene="lacG"
                     /locus_tag="ACG8OO_000328"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFP9044266.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            52826..53143
                     /locus_tag="ACG8OO_000329"
     CDS             52826..53143
                     /locus_tag="ACG8OO_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9044267.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53156..54829
                     /locus_tag="ACG8OO_000330"
     CDS             53156..54829
                     /locus_tag="ACG8OO_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFP9044268.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            54843..56474
                     /locus_tag="ACG8OO_000331"
     CDS             54843..56474
                     /locus_tag="ACG8OO_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFP9044269.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            57346..58536
                     /locus_tag="ACG8OO_000332"
     CDS             57346..58536
                     /locus_tag="ACG8OO_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFP9044270.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            58670..60859
                     /locus_tag="ACG8OO_000333"
     CDS             58670..60859
                     /locus_tag="ACG8OO_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9044271.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            60856..>61153
                     /locus_tag="ACG8OO_000334"
     CDS             60856..>61153
                     /locus_tag="ACG8OO_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9044272.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFI"
BASE COUNT        19014 a        11157 c        12121 g        18861 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25201 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25261 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26641 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct ccttcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
    29401 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
    29461 ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc agagctagtt
    29521 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
    29581 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
    29641 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
    29701 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
    29761 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
    29821 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
    29881 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
    29941 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
    30001 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
    30061 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
    30121 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
    30181 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
    30241 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
    30301 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
    30361 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
    30421 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
    30481 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
    30541 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
    30601 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
    30661 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
    30721 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    30781 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    30841 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    30901 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    30961 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    31021 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    31081 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    31141 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    31201 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    31261 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    31321 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    31381 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    31441 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    31501 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    31561 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    31621 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    31681 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    31741 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    31801 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    31861 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    31921 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    31981 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    32041 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    32101 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    32161 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    32221 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    32281 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    32341 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    32401 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    32461 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    32521 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    32581 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    32641 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    32701 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga
    32761 agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt
    32821 ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa
    32881 taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt
    32941 aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg
    33001 cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg
    33061 gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact
    33121 gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt
    33181 tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga
    33241 aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt
    33301 ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat
    33361 cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga
    33421 taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt
    33481 aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata
    33541 cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga
    33601 tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg
    33661 aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg
    33721 tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt
    33781 cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt
    33841 aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt
    33901 agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga
    33961 tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact
    34021 aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga
    34081 attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa
    34141 cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc
    34201 tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg
    34261 ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata
    34321 cgataataat aacttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc
    34381 acagtccctt tttccgaaca aacggtgttc aaaagcttgc atctacggca ctaagcccaa
    34441 tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc
    34501 agctttttca caacctctcc actaaacggt gttctcctgc caactcctcg gtcacaagtc
    34561 acaatattca acaaacatga gaagctgttt tttgaaattg ttccttgtga ctcggagcta
    34621 aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc agctgaccct cggtattagc
    34681 tcaaaaaacg gcaaaatatg aggaactatt ttttccgttt ttttctctaa tactcaggtc
    34741 aaaacgctga tttcacaacc tctgattcac ggtgttccag aagtagcttc tacggaaata
    34801 agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    34861 gctgaccact tctgtcacaa cctcttattt tgattagaac ttgcgaaaat aaaccggtta
    34921 aagtgtgtct agcgcaagag atttgctata atagacgata agggtaaaat gtaacgagaa
    34981 ggggttgaaa aatgtgtatc aaattgtaac agattcttgt tgtgatctgc cataccatgt
    35041 attagcaaaa gaaaaagttg attttttatc aatgaatctc caattgaatg atcaagagtt
    35101 agtagatgac ctcggaaaga cattcaatta tgaccgctac atgaatcagc tgaaaaatgg
    35161 tgcgatgcct acgacatcac aaatcaatgt tggccgatat ttagaatttt tccgttctta
    35221 tgcggaaaaa cagattcctg ttttatatgt ggctttttcc agtggattaa gcggctctta
    35281 cagtagtgcg cttcaagcgg ttgaattgct gaaggaagaa tatgaagacc ctgagatcta
    35341 tgtttttgat acaaaagctg caagtattgg ggaagggctt ttagtattgg aagctgtaag
    35401 attgaaaaaa gaaggtaaaa gcatcgaaga aaccctagag tggctggaaa gtaattattt
    35461 gaaagtccat tcatgggtaa cagtagacga tctgaaatat ctagaacgag gcggtaggat
    35521 ttccaaagca agtgctactc ttggcagtct attgagtgta aagcctatta ttacggtgga
    35581 taaagaagga aggctgacga atgttgacaa agttcgagga agaaacaagt cgatccagac
    35641 aatcgtcaat gagacggtaa aaacaatcgt tgcaccactt gatcaaatcg tttatgtggc
    35701 ttacgctggg gatctagaga gcgcggagaa ggcaaaacgg cttttggaag agcagataaa
    35761 gatgaaagat gtgaagcttt atcccttagg accaacgatc gccagtcaca caggctatgg
    35821 ctgtatcgcg attttttcga tgggaatcag tcgataaaag cagtaactca ttctgtaaaa
    35881 aagcccagaa attgattgta acaaaaacag cgactcttaa aagatagatg agcatcctat
    35941 ctcttagagg tcgctgtttt tttatttttt aagctaaata ggccattatg cccataactc
    36001 gttcaattgt ttttgaatat ctgcattttc caaaaattca tcatatgtcg tatcagctcg
    36061 atcgatcaca cctttatccg aaacggcaat gatgcggttt gccaatgttt ggataaattg
    36121 atggtcatgt gacgcaaata agattgagcc agtaaatgcc atcaagccgt cattcaatgc
    36181 tgtgattgat tctaagtcta aatggttcgt tggatcatct aatacaagta cgttagcttt
    36241 cgaaagcatc atttttgaaa gcatgcaacg gactttctct ccaccagata aaacattcac
    36301 tggtttcaat acttcttcac cagaaaacaa cattcttcct aagaagcttc ttaagaaggt
    36361 gttatcatct tcttctttac ttgcatattg acgcaaccag tctaaaatcg ttagatcagt
    36421 atcgaattct ttcgttgtat cctttggtaa ataagaatta cttgttgtca caccccaacg
    36481 tactgttcca gtatctggcg taatttcacc catgataact ttgaatagcg ttgttgttgt
    36541 tatatcattt tctgcgacga aagcgacctt gtcttctttg ctcaaattaa aagatatatc
    36601 atctaaaatt ttctttccat caatcgttac acttacattt tctacctgca ataagtcgtt
    36661 tccaatctca cgttcaggtt tgaatccaac gaatggataa cgtctagaag aaggttggat
    36721 atcgtctaaa gtaatttttt caagcatttt tttacgtgaa gttgcttgtt ttgattttga
    36781 tgcattcgca ctaaagcggg caataaattc ttggagttct ttgatttgtt cttctttttt
    36841 ggcatttgcg tttgcttgaa gtttagccgc tagttgacta gattccaacc aaaaatcata
    36901 atttcctaca taaagtttga ttttaccaaa atctagatcc gccatatgtg tacatacttt
    36961 gttcaaaaag tgacggtcat gggatacaac gatcacagta ttttcaaaat tgatcaaaaa
    37021 ttcttccagc caattgatcg attggatatc cagaccgttt gttggttcgt ctagcaaaag
    37081 aacatcaggt ttaccaaata gtgtttgtgc taataacact tttacctttt gtccaccagt
    37141 taattcactc atttgctgat ggtgcaattc ttcagggatg ttcagacctt gcagtaaaac
    37201 agctgcttct ggttcagctt cccatccatt aagctcagca aattctcctt ctaattcagc
    37261 tgctcgaata ccatcttcgt cagtgaaatc ctctttcatg taaatcgcat ctttttcttt
    37321 cagcacttca tacagacgct tgtgtcccat gatgactgtt tcaagtacag tatagtcttc
    37381 atagtcgtaa tggttctgtt tcaatacggc catccgttca tctggtccca tagtaacgga
    37441 acctgtggaa ggctgcagat cccctgataa cactttaagg aacgttgatt tacctgcacc
    37501 atttgcacca atcaagccat agcagtttcc gggggtaaat ttaatattca catcatcaaa
    37561 tagtttgcgg tctgaaaagt gcaaactcac atcatttaca gtaatcaatt tttttcctca
    37621 cttttatcat tattagtttt gacaagtctt atagaattat ataggggaaa ggtaaaagat
    37681 tcaaggcaat cgaacattct ttgtgtttca cgtgaaacag aatgaaaaaa ataaaaataa
    37741 aagcggagcg aaaatagcta ctataggatt tattagtttg caacagcata atttttgacg
    37801 ttatttataa gagggagtac tataaaactg taaaagaaat gaaagaagga ttgaaaatgg
    37861 atgaaaaaag aaccatcgac tggggctcat tgattttggg aattttattt gtacttgtct
    37921 cattgttatc atttcaagac cctgtcggaa atttagttgc aattgtagta gtctttgcga
    37981 tttttgcctt tattaaaggg atatttgaac tgtttgtacg gaacagatta aaagagctaa
    38041 caggatataa aggaaagatg cccttaataa tcgggatcat cgatattcta gttggcgtct
    38101 ttttcttatt caatattggt gcaggagtcg cagcattgcc ttttgttttt gcagtatggt
    38161 ttattgctga ttcagtttta gcgttattta ctgctgactt ggcaagagga gtaagtgaag
    38221 gatattattg gtttacgatc atcgtcaata ttttaggaat tttattagga attttcttat
    38281 tgtttaatcc aatttcttct gcgttgacat taagcttttt agttggattc tattttatgt
    38341 tatttggtat cacacatatc gtctatgcat ttagataagt aaaatcaaac aaataaaaaa
    38401 gccggcttcc gattacagtt ttactgttgt aattggaagc cggctttttt atcatggaca
    38461 aatatgcacg aggtgatttt taggtgtcga gacaaattta tgcaaaaatg tgagaagcta
    38521 tatacacaaa ttttatctta atactcgaag catagcgcag ttgtcacaac ctcttttcat
    38581 ataattcatt caatactggc aaatcatgga cttcatattt ttctttgaat gcttggattt
    38641 cttcttcaga atattttctt ggatctaaat gcagatcttc tactggcaat gggcgcttgt
    38701 tattgatctt cacatcgatt actacaggac gatctgattt tgcagctgca tcaaaggcaa
    38761 atcgaagttg cgagtattct gtgactgtaa agctatttgc cccgagcgct tctccaactt
    38821 taccgaaatc tgcatcttct aaaaatacac caaatttctt ttgttccgta tcttcttgtt
    38881 ctgcttcgat gaaaccaaaa gaatcattcg aaaagacaac attaataatt ggaagctgat
    38941 atttgacttg tgtaatgata tcttgcatga ccatagcaaa cccgccatca ccacttaagg
    39001 taaatacttg tttttctggg tagcttagtt gtgcggcaat tcctcctggc acaccattac
    39061 ccatagttgc gaaccatcca gatgtcgtat gtttttgttt accgttcata ttcaataaac
    39121 gaatagaatg gatagtcgtg ttaccgacat ctgtgacgaa aatcgcatcg tcttcagcaa
    39181 tacgattgat ttctttatag acagcctctg gacgaatcgg ttcttcctct cgatcttcaa
    39241 aagatttcaa ccaatttacc catttttctt tattttcttg atttgctagg taccagctat
    39301 cagcagggcg agcatcccct aactcgacaa gtctttgcaa agctgtgttt gcatcaccga
    39361 ggatcgacaa agatgtttca tggcgtcgtc caaattttga cgcatcgata tcgatttgga
    39421 taaattcagc agttggatta aaaaatgcac gtccaaatgg gaagtcgcta ccgacaaata
    39481 agatcagatc ggcttctgcc aatgcttcat ttccgggttt agtagcgaca cgtccagcaa
    39541 aaccaagaaa gttttcataa tcatcaggaa taattccttt tgctagaacg gaagcgacaa
    39601 caggaactga aaaatgttct gagaattctt tgatttgcgg aaatccgttt cgcgttcctt
    39661 gaccaacata aagaactggt ttttttgctt tttcaagata aggcaaagca gctagcaaat
    39721 ctttttcttc tggtaatatc acacttgttt tgtgagtgtg agcggtggag aaaggttgtt
    39781 cgtcaatttc ttcaaaacca agatcaacag ggatagtcac gatagccacg cctttatgct
    39841 cataggcggc tttaatggct tcatctacta catgcggaag actttgtggt gtcatgactg
    39901 tacgattgta tacgctaaca tctgcgaaaa taggattttc atttagttcc tggaaagagt
    39961 tatagttcat ggatgtagaa gcaacttgtc ctaagaacgc tactagaggc acatggtcca
    40021 tctgtgcatc atacaaacca ttgatcaaat gtgtcgcacc aggtcctgca gagccgaaga
    40081 cagcgccgac tttccccgtt aattttgcat ctgcagcggc tgcgagtgcg ccgacttctt
    40141 catgacgaac ttgaatgtag ttgatttcgg ttttttcttg atatagcgca tccataattg
    40201 aattgaaaga tcctccggga atgccgtaaa tatggtcgat accccagctt tcaagaactt
    40261 ttaccattgc gattcctgca ttgatttttg acatggtgtt tcactccttt agcaatgatg
    40321 atacataaat tttttgtatc tagagtatag aactttttat cgaaaatccc aactaattcg
    40381 tcttttcaca aaaatgtagt catgtttttg ggaatatgct agagtagtac atagatagat
    40441 tcaagagagg ggaatggcat ggttgaatgg ctattgagac ttgaaccttg gcagcaagca
    40501 cttgctggga cagcttttac ttattttatg acagctcttg gtgccgggtt ggtttttttc
    40561 tttaaagaaa tcaaaaaaga agtgctaaac ttgatgttag gatttgcttc tggtgtcatg
    40621 attgctgcaa gtttttggtc tttgttagat ccggctatta caaaagcaga agaaaatgga
    40681 gatattgctt ggctagttgt aagtattggg tttggtttag gcggtgtgtt tttatatatg
    40741 gcagataaaa cactgcccca tatgcatttt ggaccacaac atgaagcaga aggattgcct
    40801 acccatttaa aacggacgat cttgcttgtt ttttctatta cattgcataa cattcctgaa
    40861 ggtttagccg tgggggtagc attcggtgca gcagcaactg cggataatcc gacagccgct
    40921 atattagccg ctgtttcggt ggcgctaggt attggtatcc aaaacttccc agaaggagca
    40981 gctgtatcaa tcccgttgcg acaagaaggt ctgagtcgta aaaaggcctt tgtttacgga
    41041 caagcttctg gaatcgttga accaattgct ggagtgattg gagcgctact agtaacgaaa
    41101 gtggagctgc tgcttcctta tgcacttgct tttgccgcag gagcaatgat ttatgtcgtg
    41161 gtggaagaat tgatccctga agcacagcag acattgtcca gcaaacgtca ttttgcagtt
    41221 tttggcgtca tgagtggatt tatcattatg atgattttgg atgtggcatt aggataaata
    41281 aaaaaaatat atatgatata tcaaataggg ccgcgaactt atatcgcgac cctatttttt
    41341 tagtaagtta tatcataatg aagtgaatgg tatcatacat taataatagt tttttctatg
    41401 tgtatttcct ttgatccagc gaacaagcca gatgataagt gcgattggga ttagaacttt
    41461 gaccgcaaac cataaaagcc cgcttaggac actgacaaaa agactgatga cgaagctagc
    41521 tgcaattagc actaaaagaa ccatgaagat atttaagaac atattttttt gacgcataca
    41581 aaaccctcct cttgttcatt ctttactata gaaaaagtat aacagggatt gtttaaaaac
    41641 acctccaact gaagactgat ttttacttca tctacttaaa tgatcaaaca attgcttttt
    41701 ctctggttta tcatctaaaa tagagcggaa aaggaggaga atatcatgcc taaaaatcgt
    41761 cttgaagcat ttacagatgc tgtaatcgcg attatcatga ctattttagt tttagaactt
    41821 catgaaccta acggagacac gtgggaggcg ctcgctgcat taggttaccg attttttatc
    41881 tatattgtca gttttatcat tttagcgatt tattggaaca atcatcatca tttatttcaa
    41941 gttgtacata agataaatgg acgggtctta tgggcgaaca gtttctttgt ttttgcgctt
    42001 tcgctttttc cgtttgcaac ttcatggatc agtgagtaca tcaatagcct ggctccagaa
    42061 atgacttatg gttttgttac cttgaccgct aatattgcgt attatatctt ggcaaaagaa
    42121 cttgtcaaag caaataaagg ccacgagaaa gcattgaagg cgatgttgaa taattacaag
    42181 aaatcgtatc tatcgattgg tttgaatgtg ttaggaattg tcttagggta tttagtagcg
    42241 ccagtagccg tgctgatcgc aaacatcgtg attctagctg cttggatcat cccaagtcgt
    42301 accatcgaat cacaatatcg gtaaaatgat ccgtgtttaa aatttaagag aaaaatatag
    42361 aaaaaataaa taaacgatca aatatccgag tgatactagt attcaaagag ggatgcagat
    42421 ggatcaaaaa agaattcatg taggactgat tactagtttt gtcacgatgc ttatttattt
    42481 ggatcaaggt catttgactt ggacagtagt tggtgtcgta ggatttttga cattattcac
    42541 agcaataaaa aaataaacat ctttatgtaa tctgatgttc tatctattta agaaaagcat
    42601 gtttacagca gtatgattct gttgtaaacg tgtttttatt tgtggaaaat ttaattttta
    42661 gaaaagtgta tcgaaaaata gatagttagt ttttccacag tggataactt cgtaagagat
    42721 aaaaataaaa aattagtcaa agaagggcgt atagatttct tttgatagga gatatacaac
    42781 agaaatgaat aaatattaac gtgttttcaa acagtataaa acggtgtggt ttttggaata
    42841 aaaaaataac atatctcatt ttacgctttt gttattttat acttaaaaaa taacgacaca
    42901 gaaaataccg tgtctcagat ttctctacta cttctaaaaa ctactgtttt tttcctttga
    42961 aatgccttca aaaagtagga tcaggcagaa tatactggca gaatcaagga gaagggagcc
    43021 taaataagga gaaaaaccat agaaaatata aagtaattac ttataaaaca agctttttat
    43081 agaaaaataa tatacctgag tagggtataa attaagacgg aattcctttc tagattgaga
    43141 tattgactag gaagaatgtt aagattttaa gcctttaagc taaaaaaaaa ccctttttgt
    43201 taaataaggg gaatatagcc tgtcagtttc aaactgcaag tcgtcctgct gcaataatat
    43261 agtagattag agatgaaaat agattttttt aaaccaatga ctgaataaaa agaccgtcat
    43321 agatacacat tggaatgtgt ctattacggt ctttttttac gaacgatttt attgaagacg
    43381 gacagttcgg gtcaaaaata aaaaagctac gatcagaaat aagaataaaa tgtatcccaa
    43441 aataatggct agacggtcta acttatttgt cgatagatgt ttgtttagaa gttgataggc
    43501 ccataaacct atcagtcctc ctgttgtatt agttatcaaa tcagtgatgt ccgctacgcc
    43561 aataccgaat aggaattgaa tcaattctgc caagaagctt acaaggaaaa taatccctaa
    43621 ttttttagaa aaagaaagtc tcttgaaatt gacattcaaa agcaccccaa aaggaaaaaa
    43681 gacaatcaag ttataagtgg tctcacgtaa taccgtcgta ttttcaaaag aaaaagtaga
    43741 aaaagggacg agattgacat tgctgtaaga ataagtgaga aattgtggaa gattaaatga
    43801 tagcttgaat aaaatcaacc ataatagaaa gaataaataa atccctaata aaaattttga
    43861 tacataccgc atagacatct cctccattta aaaagtatac catcgaaaac cgctgaaaaa
    43921 tcataaaaaa gtatgaagtt taaggaccaa cgtagttatc gtacgaaaat tagaagcttt
    43981 tttttgttgg gaatccggtt tctttaacaa agctaagata gcgagttgct tggaaaagtt
    44041 gctatctttt ttctgtcaaa acgactcgga atgttcgttt tttgttctaa tttctccaag
    44101 ttgtttactt tttgatacta agtttatgaa aaatcaagaa atagttgtta tactaggtaa
    44161 tgctgacaaa cgggattggc atttcatctt ggttcagctt tttttccaat tactaggaag
    44221 gagggataga ttgttcaaga cttattttcg tttcaataaa atacaagata atccttttcg
    44281 tatcatgaat ctgatgctac ttatctttat tgtattatgg ttaggttaca taaatcatga
    44341 ttttttgatt tcaagttttt catcattagg gatctttact tttttctatt atcaaaacat
    44401 accgatgaaa tatttgctga aacgtatgtt tctgatcggt tgcggactgt tggttgcttt
    44461 cgttttgggg atcttatcga cttatgtttt atggcttgaa ccatttgccg tagctttggt
    44521 tgctttttcc agcagattcg tgttgcgatt attccatatt tctaaaccag gtggcttgtt
    44581 ttttgcgatg ctttctgcca tgggaacgag catgcagctg ccaatcgctc aattaccgat
    44641 cgtctcactt tattttttta tgggtgtcgt ttttgccctg attgcggcag tgattacaaa
    44701 attactggat tcacggcctg agcagacaat tgaaaaagca actttaaaag aaagatttca
    44761 cgaagagcct ttagtgatca ttgattctgt tttctacagc gctgccttat ttctttccgt
    44821 ttatgtcagt cacggcttga atctgcataa tccttattgg ttgaccttgt catgtgcatc
    44881 gattttgcta gcagaaaact tggatgcgat gaagcatcga caagtccaat atctgattgg
    44941 atcgatgggt gggttatgtg tgtcggcatt cttgtcattt gtgccgttta cgcagttgca
    45001 gaccattttt ctgatcactt ttctatatgg aatcgcacaa tttctagttg ctagaaatta
    45061 tgcagttgct aatatttttc ttaatccgat ggcgttgatg ctatctacac ttattcgtgg
    45121 agcatacttg atttctttga tcgaatatcg ttttttaggg atcgtcatag gaagtttcat
    45181 cggtttaggt gttgcgtggg taatgacggt tggtttgcaa cattatttaa cggttgttcg
    45241 tcaaaaatta gaatagaagg acaaatatat gtgccaaaag aggttatgac aagatttttg
    45301 tcacaacctc taattcaagg tgttcaaaag ccagattctc agcattaagt caaattcaca
    45361 caaaaatgtg gaaaggaact ttcaaaaaaa ttttatattt attctatcaa aaaattttgt
    45421 ttatgatggt aactgaataa actttcctga gatggtcaat gctttccttg aaagtcttac
    45481 agatattctt tcttcataga aagtttaagt tttatagtta gtgtacaata aaagtatgtt
    45541 tataccatat ggtgtccgga taatcagtca tttaaacaat gaggttcaaa gatatgaaaa
    45601 ggggaaattt gatgaagaaa ataagcaatg aaatccaaat tctggcagaa actttaactc
    45661 ttcccaaagt cgttatagag caaatgattg tacaagcaaa aaacatcctt gatgaggtgt
    45721 ttgagcaata tagtcatgaa ctaatggatc ctaataaagc aaaggcgact gcaaagaaaa
    45781 tagacaaagt ctatgaagag gaaccttttg cagcattagc tatttatcta tatgcagctt
    45841 ctcaaagttg gaaaaaaatt tatcaaccat tgaagattcc aagaactatc tatttggcaa
    45901 caatgaatgc ttttactaga tttattcatg aacatttcca agtaacagga agctatcgct
    45961 ttgaccgtgg tttttggatt tggcattaca ctagtggctt gatctttcgg attggtgaat
    46021 tggagtacga aatgacatat tttccgaaag ggtacacgga tctaagatta gaaggaaagc
    46081 agtgcctttc tcttcatatt ccttccgatg cagatttatc tcagaataag ataagagata
    46141 gttacgaaag tgccattcgt ttttttgaac tttattttcc tgattaccat taccaagtga
    46201 tgtacacaga tacttggtta ctatcaccca atcttactaa atggttgaaa aaagagtcaa
    46261 aaatctgtct ttttgcagcc gattatcgat tattatcagt tgatgaacag gacgatagtg
    46321 gagtcccttg gatctttgga cgagtagatg cccaaataca tgattatcca gaaagtacga
    46381 gtttacagcg acaagcaaaa gagcaattgc ttgcaggcga gcacattggc tctggcttag
    46441 gtattcttac ttcataagtt tagtacttcg aaatagttta aaagagcgtg agacaaaagt
    46501 aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc
    46561 ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta
    46621 tttcttggag ttaaacgctt ctgtcccagc ctctttttta gtgttctatc gaacacacag
    46681 tacgttcaat aggtaaacta gctataacaa tagatattaa agttaacgga caagagggcc
    46741 ttttttaggt agctaaaacg taataatgaa aatttatttc cagagatagg agtcaatcag
    46801 ctagtctgat cgactgtttt aacaagttga ctttcatttt aacttttgat accatttaaa
    46861 acacaaatcg taattgtttc gatttgtgtt ttttagcatg actacaatca aagaagaaag
    46921 tgggtcgtcc atatggaaaa aacagatatc tcaagtgcat accgccgctt gaaaagccca
    46981 aatatcaaaa cacgaaaacg tgcgctgaag atcatcaaag atgtgaaacg gaatagtggg
    47041 aaaaggtaag aagaaggaaa cttaattaaa aaaatagccg aaaaacgttt ttcggctatt
    47101 ttcaataatc ggtttttgtg taaggtttcc gaaagaactg ctgttgattt tccattttta
    47161 acatatgata aattggtaga atctttcttt tcaaattgat accattccct aattccagtt
    47221 atatagttaa ctacagaacc acaaagcacg tacgtttata aaagtattat ctgtagttat
    47281 ttttcaattc cctttcaacc tcttattttg accagtagta ttgatcactt taccttttaa
    47341 cacctttgtt cgtttttgaa acatttttag tcaagaatgg aaacgtaact acataatcaa
    47401 aggttttgcg aaatatttca tgaaatattt catgtagaag cgagcaatac ggttgcaaat
    47461 aaccattatc gtttaataag cagttacgaa agctcccaat atctctaacg ttcagctcac
    47521 tccatttctt ttcttcaaaa aaatcagtga ttcgtatctc tttctatttg tcgaaaaagt
    47581 taactttaaa aagtaacgcc gaaaaaatat gcaaaaaaat aaaaaagcag ttactattgc
    47641 tagaataaca tcaaggaata ataagttttg gttttcagat aatagtcgat aagttgtgta
    47701 ttggaatttt agatacagta ataaagtatt tttcatgaaa ttttgtgaga atcactatta
    47761 caagtatctt taataaaatt aatggttaag gaggaactta aagtagaatg taaatgaata
    47821 gttgtagtca gtcgattgtg atactagact caaagttgca ggaataagtt tttcaatttg
    47881 tgattggaag ataaaaaatg aaaaagaagg gctttttttt atactttgtt gattgtagtg
    47941 tttattttcc ctctgtttag ccaagtaaca ttttctatga ctcaaccagt ttcaagtgag
    48001 gaacatgata acctttttcg tgctacagaa gagtttaaga aatatcagga aaatattgtt
    48061 ccggagactc cattagctag tgagccaatt actgatattg atggaaacca aataggatgg
    48121 tatatacagt acgaagttat ggtggtaaat aatgactcaa tagtaaaaaa tacagtaggc
    48181 ttagagctaa caccgattga aaaggtgagt tcattgttaa cgtatattta tactgatgaa
    48241 agtgacgaga ctgagatcat tttaacggat tactcaaaaa ttgctactga taaaaagtat
    48301 atattgtgga tgtagacact agtgaaatac tttatgatat agaaattgat gaaaatttac
    48361 tagatgtagc acaagaaatc gatcatcaaa agcaggaagc tattcgatat gctggtagaa
    48421 aagggaaaag aaaatattta ttacgtgaat gtagcaaaag ttagagaaga tgaaaatgag
    48481 tggaaagaat tcaagagtcg ctactcaatt aactcgactc cgacatttac agtttacaga
    48541 gaaggatcga tagaaaaaac tgttttttgg actaaagaaa gtggaatgtc tttagctgaa
    48601 gtagaagagt ttttggatta tgttagtatg caacagtaag ggagttctta ttatgaaaaa
    48661 ttttgacaca tattatgtta ttgttttttc gatatctttg ctgttaatat tactaacttt
    48721 aatgcagtat aaagagtatc cagagtattt tattttaact tttgtatatt tagtaagtag
    48781 ctacacactt ctatataaat tcagaagttc ttctagagtt tctaaagttt gtttcttaat
    48841 tagcttattg attctataat atttttgatt tatataggaa attaaaaata ttatagaatc
    48901 aacactcaga gaacgggttg gtttttaata gatttaataa gtactgataa tgaaatttct
    48961 cctggttggg aaaacgaagt tggttaccct gtagaagaat tttttcaaga ttactttgcg
    49021 gagtatgccg ataggataat gagagattac gtaacagata aaactcaaga agtcatcaat
    49081 caatcccagt cattaaatga attatctgat aaggcgaaag aagaagcagt tgccattatt
    49141 tcttcaggga ttagtgacta tattgatatg agtggtgaaa tcaattatgg cgataaagac
    49201 aaaattgtac gcatgataaa agcttttatc aaaacttttg atgtgaaggc agatcttaag
    49261 tttaatgata cttacctagt aggaaaatta attaatatcc ttgggacaga gaaagatacg
    49321 gaaacgttta tttcctcttt gtctatgttg tttacagata agcatttttc aactcagctt
    49381 ggtggagagg aactagtgga aatcttttaa ccattacgcc cagcattaat aaaagtatat
    49441 caaaaaagta ctcctgatga tttttaagat tggtttgaga aataatgtga aaacactatt
    49501 tttaatgtta attatagata gagatgaacg cgtcatatgg ttaaaagaat gaaaaagtca
    49561 aagatacact gctatatttc actaaggata tagcagtgta tctttcatgt cgtttcactg
    49621 gcagtttttt ataaaaggag gtgtgcattt cagttgcttt tctttggtag ttagttgaat
    49681 cgtttaagaa ttaacggatg tggcagacag gtgctctgtc caaaaaccag taaaatcttt
    49741 gatggttcca tctttattta gctgtgcctg ttcaaatact gttttgtttt taccgaatgg
    49801 atcaattgca ataattgttc cgatccccgc acgtttggca gcaagtaatc ctgagtaagc
    49861 atcttcgata acaagacaat cagccggagc taagctcagt ttgtctgccg ctttaagaaa
    49921 aatatccggt tgcggtttcc cagggaaaga gccgtcgtca tagacaatct tgtcccagtc
    49981 aaaccagcgg gctaaatcga aaatatcaaa ataaaaagca acattctctt tgacagtagc
    50041 cgtggcaatc gttaatggaa tctgttggtc agtcagctgg tcaagagtcg cttcaagccc
    50101 ttttgtcaac tgaagctcgt tttcattttg caagcaaagc atacgataat agctttcttt
    50161 ttcttctgac aatacggcga tctcagaggc ggtcaaatgc tctgaaatga aatgtttcaa
    50221 aatctccgta ttcgtccgac catgaatatt ttttaaaata tcctcgtctg ataaaccatc
    50281 ttttgtatga tcatgtatca tatgcagcca agcagcttca tgcagatgag agtccaaaaa
    50341 cattgttccg ttgaaatcaa atataatccc tttcatagtt acgctcctat agtggtattt
    50401 tcggttgaat cagcaatttg cagatcgaca tattgtgcat agtgctcttg aacctcccgc
    50461 gagatctggc gatccgtaat gagcagatcg aaatcatgca aattataaaa cacataaaaa
    50521 tcatctcgat tgaatttttt attatcaacg agtagatatt tcttacggga attatttaga
    50581 gcgatctgct gtgcttctcc ttcttcggta ctgtaagtag agatcgcttc gttgtgaacg
    50641 ccatttgaac tgataaatgc ttttgtaaac tttagtcttc ttaaattgtc gttggtgatc
    50701 ggtccgacaa atgcgccggt atttttccgg aaatcaccgc caatcagtag aatttctggt
    50761 ccatctgctt gagcaagaat atcaaaaact ggataattat ttgtaatgat ccgaattttc
    50821 ttgttcagta aatgcgtcgc caatagttcg attgttgtac ctggtcctaa gaaaatcgtt
    50881 tctccctcgt gaatcaagga ggctgctgtg gcagcaattt cttttttttc ttgaatctgc
    50941 agagtggctt tttcagtgtg agaaagttct tgatctaaag tgtaggaaag actttgagcc
    51001 cctccatgga cacgcagcag cttgcccgat ttttctaatt catctaaatc tctgcgaacg
    51061 gtcatatcag aaacatctaa ttgattgatg atttcattaa cggtaaggat tcctttttta
    51121 ttgaccattt ctacgatagt aagcaatctt tcttttttca acattgtctt cacctctaat
    51181 ttaaggataa tctatcatat aaacggacaa cagtcaatct aattcgaaca aaagtaaaca
    51241 tttagggtgg tttttatgtt tgttacaata gattttattg ctgttttctg gtttttatga
    51301 ttatttgtgt ttgtttttgt tgacaaatgt tttggatgcg tttacaatgc aatcagttag
    51361 aaagagcgaa aactgattat agatttgagg agagatgtac atgcttaaat taccagaaga
    51421 ttttattttt ggcggagcca cagcagccta tcaggttgaa ggagccacta aggaaggtgg
    51481 caaaggcgct gttgcttggg atgacttttt agaggaacaa gggcgattca gtcccgatcc
    51541 ggcaagtgat ttttatcatc aatatgcaaa ggacattgaa ttgtgtgaac gttttggtgt
    51601 aaatggtcta agattatcca ttgcttggag ccgcattttt cctgatggag ctgggaaacc
    51661 aaatccagaa ggaatcgctt tttatcatcg tgtgttcgaa gaatgtaaga aaagaaacgt
    51721 aacgccgttt gtgacgttgc atcattttga tacccctaaa cgtttgtttg accatggaga
    51781 ttttttgaat cgagaaacaa tagaagcatt tgtttcctat gccatttttt gttttcatga
    51841 attcaaagaa gtcaaggtgt ggagtacctt taatgaaatc tatccagttg caaccaatca
    51901 atatttgtta ggtgttttcc caccaggaat caaatatgat tttacaaaga tcgtcgcctg
    51961 tcttcataat atgatggttg ctcatgcccg tgtagtgaac tatttcaaag aaaacgaact
    52021 tccgggtgaa attggtgtag ttcactcatt agagacgaaa tatgcggcaa cagacgcgcc
    52081 ggaagataag catgctgcct ttttagacga tgcattgtcg atccgtttct tattagatgc
    52141 tacctattta ggctattatt caactgaaac cttgactgct cttgatgaga tatgtgaagc
    52201 aaatcaggcc agctatcatt ttcctgaaga agatttcgtt gaattgaaaa aagcatccac
    52261 gcgtaatgac tacttaggga tcaatcacta ccaatgccat tttgtcaaag cttacgatgg
    52321 agaaaatgca atccatcata acggtacggg tgaaaaaggg acctctgttt ataaagtgaa
    52381 gggaatcgga gaacgtattt ataaagaagg aattcctcga actgattggg actggctgat
    52441 ttatccagaa ggtctatatg acttgttgct gcgaattaaa agcgattacc cacattacaa
    52501 caaaatctat atcactgaga acgggatggg gtataaagat cagtttgaag atggcattat
    52561 tatggatcag ccgagaatcg actatttgag agtctatttg gaatccttaa gcaaggcgat
    52621 aacagccggg gtaaatgtga aggggtattt tttgtggtcg cttatggatc ttttctcatg
    52681 gacaaatggg tataacaaaa ggtatggctt gttttatgtt gattttgaaa cccagaagcg
    52741 ttacccaaaa gaatcagctt actggtacaa gttggtcagt gaaaccaaaa cgattattta
    52801 ataagtgata atggaggaag aaaagatgaa taaagaggaa ttacaaatgc ttggttttga
    52861 gatcgttgca tattcaggcg acgctcgcag tacattattg aaattattga aagaagtaag
    52921 acaaggaaac tttgaacatg ttgattcggc gttgaaagaa gccgacgaaa atttaaccct
    52981 tgcacataat tcgcaaacaa aaattttagc ggaagaagca tctgggaaag agatggagat
    53041 gggattcatt tttatccatg ggcaggatca tttaatgacc actttattgc tgagagatct
    53101 gattcaagac tttattgtac tgtacagaca gaatcaagga taggagaaga acgctatgga
    53161 tggaatcatt aaacaaatcg aaaaagggaa accatttttt gaaaaaatct cacgtaatag
    53221 ttatttagga gccgttcgtg acggattttt aacagcgatg ccagctattt tattttcaag
    53281 cattttcatt ttgattgctg cggttcctga gatttttggt taccaatggc ctgaatctgt
    53341 ctccacatgg ctatggaaag tctataatta ttcgatgggt gttgtagggt tactcgtttc
    53401 agcaaccact gctcgctgct tggctgattc gatgaatcgt cgcttaccga aaaacaaagt
    53461 aatcaacacc acctctgtga tgctggcctc tgtggtaagt ttcctgttgt taagcgtctc
    53521 tccgattgaa ggtggatttt caacggaata tatggggaca aagggactac tggcttcctt
    53581 tgtcgctgca tttttaacag ttaatttata taagttttgt gtgctgaaag atattaccat
    53641 taaaatgcct aaagaagttc ctggaacgat ttcgcaaacc ttccgagata tttttccttt
    53701 ctctttttca gtatttgcgg ccgttttaat tgatttaggc attcgacaaa tcttccaaat
    53761 ctcatttgca gaagcaatca ttacggtgct gcagccgcta tttacagcag ctgacgggta
    53821 cttaggaatc gctattatct ggggagcgat ggcgctgttt tggtttgtcg gtgttcatgg
    53881 accgtcgatc gtggagccgg caattgcggc aattatctat gccaatgtgg aaaccaatct
    53941 gcaattgttc aattccggtg aacaagcttc taaagtactg acggtgggtt tagggaattt
    54001 tgtaggaact atgggaggaa ctggggcaac attggttgtt ccttttatct tcctactctt
    54061 tgctaagtct aagcagttga aggcagttgg gaaagcttcc tttattccag taagtttcgc
    54121 tgtcaacgaa ccattgctat ttgcggcacc catgattttg aacccttatt tttttattcc
    54181 atttttgtta acacctgttg taaacgtttg cctgttcaag ttttttgtcg atgtattggg
    54241 catgaacagc tttatgtacg ttttgccttg ggcaactcca gcaccgatcg gtttggttct
    54301 aggaacgggc atgggtatct tagcttttgt tctagcagcg gttctaatcg tcgttgactt
    54361 cctgatctat ctaccatttt gcaaagcata cgatgctgtc ttagtagaga aagaagcaca
    54421 aaaagcagag gtgatcgcaa ccgaggaagg tgcttctgcc gatgtacgtg aggaagtacc
    54481 tgtggcagcg agtcaagtta tcagcgatgc aaataaagaa atcaatgtgt tggtactttg
    54541 tgctggcgca gggacaagtg cgatgttagc taatgcttta aaaaaaggtg catctgaatc
    54601 gaaagcaccg atcagtgcaa tggcgggtgc ttatggttct cattacgaaa tgctgcagga
    54661 ttttgatatg gtggtcttag caccgcaagt caattcttac tatgatgata tcaaagaaga
    54721 caccgatcgc ttaggaatca agctggttgc gacaaaagga gcagaatata tcggattaac
    54781 gagagatcct gaaaaagctg ttcagtttat tctgaaccaa tttcaataaa ggaggagcca
    54841 taatgaaaat gggaatttgg ttgcgtaaag ctgttttatt tgcagctgtg ttaggggctt
    54901 tgggggctgt ttcaaaaact gttgaagcat ctgccagcga atttattcgc ggagcagata
    54961 tttcgatcct gtcagatatg gaaaaatctg gtgcaaaata ttatgaagac gatatttcaa
    55021 aagatgcctt agagatttta aaaaataatg gtgtcaacta tgttcgactg cgtttgtggc
    55081 aggatcctta taatagtaat ggagaaagtt atggagcagg gacaaatgat ctggaaacga
    55141 cgattgacct tgcaaaacga gcaaaaaatc tgggacttaa ggtgctgctg gattttcact
    55201 acagtgattt ttgggttgat cctgggaaac agaatcttcc taaagcatgg caaggtctga
    55261 cctttgaaga aatgaatacg gcattgtacg actatacaaa gaacgttctg tcggaaatga
    55321 agcagctgga cgtttatcct gatatggttc agatcggcaa tgaactgaat agcggaatgc
    55381 tctggcctta tggcaagtct tggggagaag gcgggggaga atttgatcga ttggctgctt
    55441 ttttgaagtc tggtatccaa gctgttcgag acactcaacc taaaacaact ccagtaatgc
    55501 tccacttagc tgatggtggc gatacaggag catttacttg gtggtttgat gaaatcacga
    55561 gtcgaggtgt gtcatttgat ttaattggag tgtcgtatta tccgtattgg cacggttcaa
    55621 tgaatgaact tcaaaccaat atgaatgcga tcagccaacg gtatggtaaa gaagtgatcg
    55681 tagttgaaac agcttatggg catacaacag cgaatgccga tactatgcca aatgcttttg
    55741 gtgaagccga agcagctgcg gggggatatg aaccaacacc gacaggacaa gcagagtatt
    55801 tgctggatct tgccgatcgg attcaggcag taccgaataa tcgcggggcg ggcttcttct
    55861 attgggaacc tctttggtac aacggaaatg tgagttgggc aacacaagca ggtatgaatt
    55921 atctcagtgt tcaatccact atgggaaacg aatgggacaa ccaagcgatg tttgattttg
    55981 ctggaaacgc cttgccaagc ttgcgtgcgt ttaaacaagc aggggcgcaa ataaatctag
    56041 ttaaaaatgc aagtttcgaa agcgatggac caacgaccag tccttccaat tggcaaacct
    56101 ggtttcaaca aggaaccgct aagggtacga tcaaaacgga agcgggcact ttttttgggg
    56161 actacaaact aactttttgg caggatcaag catacgaggc atcagcttat caaaatgtta
    56221 gtggcataag caaaggaact tacacattga gtgcctatgt aatgtctggg ggagagtttg
    56281 atactaatca gttatatatc aaaaattatg gtggagcgga actaaatact gcggttgtta
    56341 aaacaagtga atggacaaaa attacaatca aagacattcc aattacaaca ggtgtttgtg
    56401 aaattggggt ttatagcaag gcttcaggaa atgcttggac gaacgttgat tttgtttcat
    56461 tggtgaaaca atagactgcg gatcgtttcg attcaagatc ggaggttccc ttcagccaca
    56521 tcgttagcgt agaaggcagg aatcgaacct gcacctcgcc tttgccaaaa ataaccagaa
    56581 acgatttcca gtcattttta ccaaaacgag taaagcacca gacttcatct acactttgta
    56641 ttatgctaaa gagagttcaa cgatttcgta agaagcaatg aaaaaattgc aaaaagttgt
    56701 gcagataaca taaagtttgt ttaagaaagg gtaaccttag aattccgtcg tgaatatgat
    56761 ttgagcactt agatggatag aaaaaagttc tcggaaaaca gttcgttttt cgagaacttc
    56821 tttttatgat aagaatgaaa caaattaact tatattatag tctcttttgg tagggaacca
    56881 tcttattttt aatgaaaatc aagcatttta gctattgaag aatgttgatt tatagggatt
    56941 ccccctccat attatgttgg ctctttattc ccactctgtt ttcaacgtaa agtgtataaa
    57001 acagaatgcg atactatcaa gggagttaaa acaatataca gaacagtaaa acactataaa
    57061 taaaaataaa agtgcaataa aagtgcaatt tttgcaccta atcatactct gtttatatat
    57121 gaaaactaga ttctgaatcc aatatttggt taacgagagg cgttcgaatt tgaacgtctt
    57181 ttatatatta ctttcaaata aatatgaggt agaatactat ctattttctt tttaacccaa
    57241 ttgttataat ggagagtgta aaatattttg tgtaaataga aaaaaggaag tcccttctgt
    57301 agaatagagt taccacaaca cattcacaga agagaggact tccctatgaa cgattttact
    57361 acagaaattc taaagactct agcgaacaaa ggcgatttga atgaattatt ccgtgtccat
    57421 ttggagaaag cagtcaatac gcttctcaaa acggagttaa cggctttcct agattacgaa
    57481 aagtatgatc gcattggttt taacacgggt aattctcgta acggctccta tgaccgtacg
    57541 gtcaagaccg agtatgggga acttcatctc cagattccgc gcgaccgcaa tggtgagttc
    57601 aagcaacaga ctgttcctgc ttatagacgg acgaatgaca cgttagagga gaccgtcatt
    57661 cacctcttcc gaaaaggtat taccatgtcg gaaatcgcag acttgattga gaaaatgtat
    57721 gggcattact acacgcccca aaccatgtcc aatataacaa aatcatttac agaagaggta
    57781 acggcgttta atgggcggga gcttcatgac cgttatgctg ctatttatat ggacgcaacg
    57841 tatattccgt taaagcggaa aaccgtcgcc aaggaagcta ttcatatcgc agttggcatt
    57901 cgcccggacg gatcaaagga agtattgagc tatgcgattg caccgactga atccatcacg
    57961 atttgggagg aaattttatt ggaccttcaa gagcgcggtt tgaaaaatgt cctcctgttc
    58021 atcacggatg gcttaaaggg gatggtagga gcgattagtc ggttctatcc caaagctcgt
    58081 tttcaacatt gttgtgtaca cgtttcccgt aatatcagtc ataaagtgcg tgtcgatgat
    58141 cgtaaggaag tctgtgatga ttttaaaatg gtgtatcaag cctcatctaa agaggtggcg
    58201 ttggaagcac gtggtacttt tgcggaaaaa tggaaaacca gctatccaaa agtggttgaa
    58261 tcgattcttt cgaacgatca cttgctcact ttctatgatt tccccttggc catacgcaag
    58321 agtatttatt ctacgaactt gattgaatcc tttaataaac aaatcaagaa atacagccac
    58381 cgcaaggaac agttccaaaa tgaagagtcg atggaacgtt tcttagtctc gtcttttgat
    58441 acttacaacc aaaaattcct aggtcgcagt cataaaggtt tccaacaggc cgaaggcgag
    58501 ctcgaacaaa tgctaagcca actgattgag aattagaaac aatctgcagt agggaagaat
    58561 catttacaca aaattattga cgctccctta taatgagaac ttttactaac ataatttatg
    58621 ataaacttac tatataatca tattaattaa atagttagga gatctgctta tgtttatatc
    58681 tactcttcaa attcgaaatt ttagaaatta tgaatccgaa atctttcgat tcgataatga
    58741 gacaaatact attataggtg agaatgattc agggaaaacc aatgctctta ctgcattgag
    58801 aatcttatta gatgatagtt attactatag tagtaaaaca ctaaaagaat cagatttttt
    58861 tcatggaatt caaaatgggt ggcaagggca ttggattatt atctctgcga catttgaagg
    58921 aatcagcgaa gaagaatttg acaatgagat ttgtgcttcc atttctttaa attctgaaag
    58981 tcaaacaatc ttagaagaat tgatttcaaa tgcagataaa ggtgtaggta gtatttctct
    59041 atttattaga cctaacaaag ctattagaaa gcaattattt gatatttcca ctgaattaga
    59101 ctctcatcaa tttaacgaat ttagaaattc tattcgtcta tccgactatg aattttacta
    59161 cacatctaaa tcaaatctaa atttctgtat caatgaaaat tatgataaat tggtaggaag
    59221 actcaatgac aatatagcta gtaatcctga agaagacgat gaagctctgt tgggctcaag
    59281 aattgacatg tcagatattt tcaagcatat ttcggttgtt tatgttgacg ctcttcgaga
    59341 tgttcttaga gaaatgaaaa acaatcgaaa cccagtcaaa agaatcatgg aaacaattga
    59401 atcaaaaata tcatcagata atgttgagtc attaaaaaca attatacagc aattaaatga
    59461 gactattacc agtgttcctg aaattaggaa aattggcgaa aatattaatc ggcaactaaa
    59521 tagcataatt ggttcagtgt attctcctaa tcttcttctt tcctcaacta tgtcagatga
    59581 tatgggttct ttagcaaaat ttctttcaat gaagccagaa caaaatatcg acttagactt
    59641 actagggtta ggacatctaa atatgattta tttagctttg aaaatagtcg aatttgaagc
    59701 gtgtcgttca agagaactac ttaatattat gttgattgaa gaaccagaag ctcatattca
    59761 tcatcatatt caaaaaactc tgttcgaagg attaaatctt caaaaaaact atactcagat
    59821 tctcatgacc acacattctg tacatttagc tgaagtgtct gaaatatctc ggatgaatgt
    59881 attaaaatcg tttaatggca aatctatcgt tatgaatcca gcaaataatc tgaactcatt
    59941 tgctaaagaa aagttaaaaa agaacaatct aaatctaata aacgcagtag agagatattt
    60001 agatgttaaa agaaatggat tgcttttttc taaaggtgtt atcttagttg aaggagatgc
    60061 tgaagaaata cttattcctc aaatagtaaa aaaattattt ggtataaact tagacgaact
    60121 tggaataggt ttgattaatg ttggtagtac ttcgtttgaa tatatcgcta gcttgtttga
    60181 cgatgataga atacagagaa agtgtgctat tgtaactgat ttagatgaac aggcaatccc
    60241 atcagaacat cgattgtata aaagcactgc acaagagaaa ggtcaagcaa gaaaagaaaa
    60301 attatcctcc ctttttaatg aaaacaattg ggttaattgt ttttatgcta atacaacatt
    60361 tgaaattgag tttttagatg tgaataaaga aaatattcta acttatgtca gtccaattat
    60421 agatgacgca ttcaaagata aaaaaacaat aactgcttat aaagaagaaa tacaaagtga
    60481 acaatattta gaaagaaatt tagcgatgtt aaaactagcc aatcatgttg gtaaggggtg
    60541 gtttgcaatt gagttaggtg aaaaaattga ctttttaatc aaaattccta agtatattct
    60601 taatgctatt gcttttgcat gtcaggaaat agtagatctc tcaatttata aaaaaattct
    60661 actccataca atcgacttgt acgaggaaac tacagaattg tcagctttat cagctagttt
    60721 aaaaacaagt gatacagctg atttgaatat agcaataatt tctttattag agtactttgg
    60781 taaagatgag gatgttgtgt atctaatcaa tcagatagaa aattacacaa aaatacttta
    60841 tgctgaggtt tcaaaatgac aaatatcttc gaaaaaatcg taaatgattt aaatttagaa
    60901 caaaaagatg ttgtggaaag tgaagataac ctttatgtaa atgcatgtcc tgggagtggg
    60961 aaaacaaggg tacttacaag gaaaattgct taccaagcaa ttaaacacga tggatcacta
    61021 aaaagaataa ttgctatcac atacactaat agagctgctg aagaaattaa ggaacgccta
    61081 agtttactta gtatagatga tgatattaat atttgggtgg ggacaataca tcagttttgt
    61141 cttgaattta tta
//
LOCUS       DAZKBH010000005        53774 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000005 DAZKBH010000000
VERSION     DAZKBH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.005"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..372
                     /locus_tag="ACG8OO_000335"
     CDS             <1..372
                     /locus_tag="ACG8OO_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044273.1"
                     /translation="RIYYRYLDEFYRIFASKLSSILSDKINVKKSFFKKETPVEKISV
                     ELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIATLIY
                     SVTDRHWQLVEDADLSYLPLK"
     gene            complement(453..1022)
                     /gene="thiT"
                     /locus_tag="ACG8OO_000336"
     CDS             complement(453..1022)
                     /gene="thiT"
                     /locus_tag="ACG8OO_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFP9044274.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1088..1190)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1196..2167)
                     /locus_tag="ACG8OO_000337"
     CDS             complement(1196..2167)
                     /locus_tag="ACG8OO_000337"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9044275.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2261..3634)
                     /gene="glmU"
                     /locus_tag="ACG8OO_000338"
     CDS             complement(2261..3634)
                     /gene="glmU"
                     /locus_tag="ACG8OO_000338"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9044276.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4137..4955)
                     /gene="purR"
                     /locus_tag="ACG8OO_000339"
     CDS             complement(4137..4955)
                     /gene="purR"
                     /locus_tag="ACG8OO_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFP9044277.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5122..5946)
                     /locus_tag="ACG8OO_000340"
     CDS             complement(5122..5946)
                     /locus_tag="ACG8OO_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFP9044278.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5919..6599)
                     /locus_tag="ACG8OO_000341"
     CDS             complement(5919..6599)
                     /locus_tag="ACG8OO_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9044279.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6622..7560)
                     /locus_tag="ACG8OO_000342"
     CDS             complement(6622..7560)
                     /locus_tag="ACG8OO_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP9044280.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7805..8656)
                     /gene="ispE"
                     /locus_tag="ACG8OO_000343"
     CDS             complement(7805..8656)
                     /gene="ispE"
                     /locus_tag="ACG8OO_000343"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9044281.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(8810..11383)
                     /gene="mprF"
                     /locus_tag="ACG8OO_000344"
     CDS             complement(8810..11383)
                     /gene="mprF"
                     /locus_tag="ACG8OO_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9044282.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11524..12330
                     /gene="proC"
                     /locus_tag="ACG8OO_000345"
     CDS             11524..12330
                     /gene="proC"
                     /locus_tag="ACG8OO_000345"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9044283.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12409..13965)
                     /gene="rny"
                     /locus_tag="ACG8OO_000346"
     CDS             complement(12409..13965)
                     /gene="rny"
                     /locus_tag="ACG8OO_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFP9044284.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14376..15425)
                     /gene="recA"
                     /locus_tag="ACG8OO_000347"
     CDS             complement(14376..15425)
                     /gene="recA"
                     /locus_tag="ACG8OO_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9044285.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15534..16775)
                     /locus_tag="ACG8OO_000348"
     CDS             complement(15534..16775)
                     /locus_tag="ACG8OO_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFP9044286.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(17015..17593)
                     /gene="pgsA"
                     /locus_tag="ACG8OO_000349"
     CDS             complement(17015..17593)
                     /gene="pgsA"
                     /locus_tag="ACG8OO_000349"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9044287.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(17802..18725)
                     /locus_tag="ACG8OO_000350"
     CDS             complement(17802..18725)
                     /locus_tag="ACG8OO_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFP9044288.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(19464..20750)
                     /locus_tag="ACG8OO_000351"
     CDS             complement(19464..20750)
                     /locus_tag="ACG8OO_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9044289.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(20743..22011)
                     /locus_tag="ACG8OO_000352"
     CDS             complement(20743..22011)
                     /locus_tag="ACG8OO_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9044290.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            22263..22724
                     /gene="mscL"
                     /locus_tag="ACG8OO_000353"
     CDS             22263..22724
                     /gene="mscL"
                     /locus_tag="ACG8OO_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9044291.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(22784..23230)
                     /locus_tag="ACG8OO_000354"
     CDS             complement(22784..23230)
                     /locus_tag="ACG8OO_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9044292.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(23323..23568)
                     /locus_tag="ACG8OO_000355"
     CDS             complement(23323..23568)
                     /locus_tag="ACG8OO_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFP9044293.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(23684..24262)
                     /locus_tag="ACG8OO_000356"
     CDS             complement(23684..24262)
                     /locus_tag="ACG8OO_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9044294.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(24339..24869)
                     /locus_tag="ACG8OO_000357"
     CDS             complement(24339..24869)
                     /locus_tag="ACG8OO_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFP9044295.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(24873..25733)
                     /gene="rlmB"
                     /locus_tag="ACG8OO_000358"
     CDS             complement(24873..25733)
                     /gene="rlmB"
                     /locus_tag="ACG8OO_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9044296.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(25726..26121)
                     /locus_tag="ACG8OO_000359"
     CDS             complement(25726..26121)
                     /locus_tag="ACG8OO_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFP9044297.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(26118..27530)
                     /gene="cysS"
                     /locus_tag="ACG8OO_000360"
     CDS             complement(26118..27530)
                     /gene="cysS"
                     /locus_tag="ACG8OO_000360"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9044298.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(27527..28063)
                     /gene="epsC"
                     /locus_tag="ACG8OO_000361"
     CDS             complement(27527..28063)
                     /gene="epsC"
                     /locus_tag="ACG8OO_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFP9044299.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(28149..28388)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28385..29845)
                     /gene="gltX"
                     /locus_tag="ACG8OO_000362"
     CDS             complement(28385..29845)
                     /gene="gltX"
                     /locus_tag="ACG8OO_000362"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9044300.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(30015..31172)
                     /locus_tag="ACG8OO_000363"
     CDS             complement(30015..31172)
                     /locus_tag="ACG8OO_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFP9044301.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(31206..32576)
                     /gene="radA"
                     /locus_tag="ACG8OO_000364"
     CDS             complement(31206..32576)
                     /gene="radA"
                     /locus_tag="ACG8OO_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9044302.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(32639..33166)
                     /locus_tag="ACG8OO_000365"
     CDS             complement(32639..33166)
                     /locus_tag="ACG8OO_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9044303.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            33396..34202
                     /gene="proB"
                     /locus_tag="ACG8OO_000366"
     CDS             33396..34202
                     /gene="proB"
                     /locus_tag="ACG8OO_000366"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9044304.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            34199..35446
                     /locus_tag="ACG8OO_000367"
     CDS             34199..35446
                     /locus_tag="ACG8OO_000367"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9044305.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            35522..35866
                     /locus_tag="ACG8OO_000368"
     CDS             35522..35866
                     /locus_tag="ACG8OO_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044306.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(35983..36309)
                     /locus_tag="ACG8OO_000369"
     CDS             complement(35983..36309)
                     /locus_tag="ACG8OO_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044307.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(36924..38126)
                     /locus_tag="ACG8OO_000370"
     CDS             complement(36924..38126)
                     /locus_tag="ACG8OO_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9044308.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            38243..38758
                     /locus_tag="ACG8OO_000371"
     CDS             38243..38758
                     /locus_tag="ACG8OO_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9044309.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(38957..39403)
                     /locus_tag="ACG8OO_000372"
     CDS             complement(38957..39403)
                     /locus_tag="ACG8OO_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9044310.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(39450..40928)
                     /gene="msr(C)"
                     /locus_tag="ACG8OO_000373"
     CDS             complement(39450..40928)
                     /gene="msr(C)"
                     /locus_tag="ACG8OO_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9044311.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(41282..41911)
                     /locus_tag="ACG8OO_000374"
     CDS             complement(41282..41911)
                     /locus_tag="ACG8OO_000374"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9044312.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(41895..42359)
                     /locus_tag="ACG8OO_000375"
     CDS             complement(41895..42359)
                     /locus_tag="ACG8OO_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9044313.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(42846..44114)
                     /locus_tag="ACG8OO_000376"
     CDS             complement(42846..44114)
                     /locus_tag="ACG8OO_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9044314.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(44345..45082)
                     /locus_tag="ACG8OO_000377"
     CDS             complement(44345..45082)
                     /locus_tag="ACG8OO_000377"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9044315.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(45149..45718)
                     /locus_tag="ACG8OO_000378"
     CDS             complement(45149..45718)
                     /locus_tag="ACG8OO_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP9044316.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            45846..46718
                     /locus_tag="ACG8OO_000379"
     CDS             45846..46718
                     /locus_tag="ACG8OO_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9044317.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            47060..47932
                     /locus_tag="ACG8OO_000380"
     CDS             47060..47932
                     /locus_tag="ACG8OO_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9044318.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            47929..49887
                     /gene="nagE"
                     /locus_tag="ACG8OO_000381"
     CDS             47929..49887
                     /gene="nagE"
                     /locus_tag="ACG8OO_000381"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFP9044319.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(50219..51070)
                     /locus_tag="ACG8OO_000382"
     CDS             complement(50219..51070)
                     /locus_tag="ACG8OO_000382"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9044320.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            51193..51660
                     /locus_tag="ACG8OO_000383"
     CDS             51193..51660
                     /locus_tag="ACG8OO_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFP9044321.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(51923..52405)
                     /locus_tag="ACG8OO_000384"
     CDS             complement(51923..52405)
                     /locus_tag="ACG8OO_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9044322.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(52505..53368)
                     /locus_tag="ACG8OO_000385"
     CDS             complement(52505..53368)
                     /locus_tag="ACG8OO_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9044323.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        15348 a        11516 c         9583 g        17327 t
ORIGIN      
        1 cggatttatt atcgctattt agatgaattt tataggattt ttgctagtaa attaagttcg
       61 atcttgtcag ataagatcaa tgtaaaaaaa tcttttttca aaaaagaaac acctgttgaa
      121 aaaatcagtg tggaattatt gaaagaaatg cgtaatttga tgcaggaagg gatcattttt
      181 tcttttgatc aagttggttt gatcgatggt aaatatcgta tcccaatcta tgtgattgaa
      241 gattatattt ggaaattaaa aggcacccgg tatcgagaag aggaaatcat cgctacttta
      301 atctactctg tcaccgatcg acactggcaa ttggtagaag atgccgatct ttcttatctg
      361 ccattgaagt agatctattt cttatcaaaa aataaaaagg aaacaagaaa ctaggacatg
      421 attctgtctc aagtttcttg cttctttttt atttacagct cctgtcgttt atctggcaca
      481 aaaatcgaag gagtcgtttg ttggatccct aataaaacga tgatcgttac aatagctgta
      541 ctcaacccgc tggcaccatt cattacgaaa gaaaagagcc aaggattcat cccccatagt
      601 gcatacgctc cccagaaaac cactcctgca ataaaatgcc aaaaatatct ggaaagtgcg
      661 ccaatcaatg caccaaacac gatgcttttt ctaatttgtt tcgtattatc gtctacatag
      721 cctttttgga catttgaagc catgagccct gctgcgcctg caaaagtata ggcaatcggg
      781 tactcaatca acacttgaac gacacttaaa taataaactt gtcccaacgg aaaatgtagg
      841 atgccccaaa tgaaacctgc cagcatccct gcctttgtcc ctcttcttaa cgcaaaaaga
      901 gtcaatggaa tctgccctaa tgaaatagaa aaactgctgc cgatctggat cggaatcaac
      961 gacaacacca tcgctaacgc tgcaacaatc gttccttcta cccaaactct attttttccc
     1021 ataaaaaaac ttcctccttt gaataaaaaa gtcacaaagg aggaagcagc tttcgaaaat
     1081 agtcaaacag ctgtttattc acaattccta cgctcgcata accgatacag gtacgaaggg
     1141 tttagaaata gattcaatct cagcccaatt ggactcccct ttgtgagttt atttattatt
     1201 tattttttgt ttcaaacaat ggactaactg gtttgttttc atggatacgt ttgattgcat
     1261 ctcccattag tccgccaaca cttacttcat cgattttgtc gattttacga tcttctggaa
     1321 gatagatcga gtctgtaaca actagacgct cgatagctga atcttctatt ctttgtaaag
     1381 cagggccgga taatactgga tgtgtacatg aagcatacac actagttgca ccagcttctt
     1441 tcaaagcatt ggcagccaat gtgatcgtac ctgctgtatc gatcatatca tcgatcagta
     1501 cacaagtttt gcctttgact tgtccaatga tattcattac ttctgcaaca tttgcttttg
     1561 gacggcgttt gtctatgatc gcaatcggag atttcaaaaa ttccgctaat ttacgtgccc
     1621 gtgtgacacc accatggtct ggtgaaacaa cgacaacatc atcgcctttg attccttttt
     1681 ctaagaaata attggcaata agtggcgcac ccatcaaatg atcgactgga atatcaaaga
     1741 aaccttggat ttggactgca tgaaggtcca aagtcagcat tcttgttgca ccagcttttt
     1801 gaatcatgtt tgctactaat ttcgctgtga ttggttcccg tgcacgtgct ttacggtctt
     1861 gacgcgcata gccataataa ggcatgacta cgttgattgt ttttgcactt gctcgtttca
     1921 gtgcgtcaat catgatcagc aactccatca agttatcatt gactgggctg ctcgttgatt
     1981 gaataacata gacatgcgat ccacgaatac tttcttcaat gtttacttga atttctccat
     2041 cactaaattg agtaacagat gattttccta actcgacgcc gactgctgct gcaattttct
     2101 ctgctaatgg gcgattggaa tttaacgcaa aaatttttaa tcttgggtca aagtaatgtt
     2161 ttgacatggg gacctccact ttctttttca caactctaga attctaccta ataaatagta
     2221 gtcatatttt ttaaataaat caagtgattt tgcaaaagtc ttagtttaga taagggagtt
     2281 tttttgcata tccttcttta ttgacttgtc ttgctctcgc aattgccagc gcatattctg
     2341 gaatattgtc tgtaattgtt gacccggcag caatcgaact gtttttagct acctcgacag
     2401 gtccgatgat attcgtgcta gaaccaataa agctgtggtc gccgacagtc gtatgatgtt
     2461 tgttctttcc atcatagttg acgaagacaa caccacatcc gacattgatt tcttctccca
     2521 gcgtagcatc accaacataa gtcaagtgac cgactttagt tcctttgccg atttgagcat
     2581 ttttcacttc cacaaagttt ccgatatgga cgttttcgcc aatttcagct tttggtcgta
     2641 aatgtgcata aggtcccaca tctgcatgtt tcttgacatg gctgctttca atgactgagt
     2701 tttcaatgac gacgtgatcc tcaatcctac tgtctacgat ttttgaatgt gacccgatga
     2761 cacaatcaga accaatgact gtatttcctt ggatttgtac acctgcttcg atcaatgtgt
     2821 ctggaccgat ttctacgcct gcatcgatgt atgtcgttgc tgaatcgaca aagcttacgc
     2881 cattgaccat atgcatctgg ttaattcgct ttctcatgat ctcatttgct ttagctaacg
     2941 cgatccgatc attgactccc attgattctt caaaatcatc cgtttgataa gcagcaactg
     3001 tatggccttc ttcttttaag atttcgatga tatctgttag atagtattca ccttgagcat
     3061 tgtttgttcc aactttgctt aacgcatcga acaaagcttc gttgtcaaaa cagtaagttc
     3121 ctgtattgat ttcttgtaca agagcttctt ctggtgttgc atctttttgt tccacgattt
     3181 tttctacgat cccaatatgg tcacggatga ttcgtccata tcctgttggg ttttctgctt
     3241 gtgccgtcaa gatcgttgca cttgcatttt ttccttggtg atattcaaaa agattgttca
     3301 gcgtttcagt agtcaataat ggtgtatcgc cactgataac taacgttgtt cctttttttc
     3361 cttccaagaa agaagcagct tgcaaaacag catgtcctgt tcccaattgt tctgcttgta
     3421 acgcatattt actgcgttct cctagttgtg ctttaacttg ttctgctccg tgtccaacga
     3481 tcgtaatcac ttcatctggt ttcgtttcac tgacccgatt aatgatatgt tctaccatag
     3541 gtttaccaga aacgggatgt aaaactttat ataattttga cttcatgcgg gtacctttcc
     3601 ctgccgccaa aataattgca taacgtgcgt ccaactgcgt cactcctaaa tatagtttgc
     3661 tgatttcagt ttatcccagt atacccattt tttcaacaaa acaagtgatt ttcttttatc
     3721 ccagcgtttt ctggataaaa aaatagatag accataacgg gcctatctat ttggtaaccg
     3781 gtgctctcct gccaactcct cggtcacaag tcacactact cataaaacat gaaaagctgt
     3841 tttttgaaat cgtttcttgt gacttagagt taaacggtag gcttacaacc tcttcattaa
     3901 acgtttttca atcagctgac cttcggtatt agctcaataa acggcaaaat atgaggagca
     3961 atttttccgt ttattgagct aatactcagg acaggacgct gatttcacaa cctctagata
     4021 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgaaaaa atgtggaaag
     4081 ccattttcac aaaatttttc ttactactcg aaactaagcg gctttttcac aacctcttat
     4141 tctgaaaaat aatttcctgg cacaaccgta atatttttcg tttgagtatc tacatctttg
     4201 acataaagca gtgacgtata atcatcgatg gcgcgatgac cgttgaattt tgattcggca
     4261 aatacagtga tgccaacaag ttctgattcg aattcttcaa tcaagctctt cattccgttg
     4321 actgttccgc cgcctttcat gaaatcatcc actacaagga cacgtgaacc acgtttcaaa
     4381 cttctcttag acagttccat cttttcaatt ctttcagaag atccagacac atagttgaca
     4441 cttactgttg atccttctgt tatttttgag tcccggcgta caataacgaa tggcgcattc
     4501 agataatagg acacagcttg ggcaataggt acgccttttg tggctacagt cattacagca
     4561 tcgatttttt caccaaggta tttagaagca atgattcttc caacttgacg cagcagttca
     4621 ggttctccta acaaatcaga gagataaaca tatcctcctg gaagcagacg gtcttgttct
     4681 gatagacgtt ctgctagaga ttcaatatat tttttcgcct cttcatatga aatttcaggg
     4741 atgaacaata caccacctgc agcacccggg attgtttcca aggttccata ccctcgttct
     4801 ttaaatgttt tcttgatgat tcccaaatct tcactgatag aagatttagc agattcataa
     4861 cgttcggcaa agcttgttaa ggaaatcaat tcatgcgggt gattcaataa atactgtgtc
     4921 atgtcaacta gacgttcact gcgacgtact ttcacctatt ccaactcctt ttaagaaatt
     4981 ttatcgtttt acattatata agtttttctt caaaaattcg agcattttat gaaaaaataa
     5041 gaaaatagtt cggtatttat taaaataaaa agaagcctat aaaaaatttt tcgtttttat
     5101 aaggcttctt ttctttataa tttagttatt ttttcttctg aaggcaacca atattcgttt
     5161 gatgatattt acaatcagga acaaagcaat aaagactaat gtgattgttg ctcctggagg
     5221 tgtatctaga taaaacgagc tagttagtcc tgacagcatc cctagaaatc ctacacaaac
     5281 actgatagca atcacgacat tgaagctttt gcctaatcgc ataccgattg ctgcgggaag
     5341 aaccatgatt gcggatatca aaagtgcacc ggcaatcggg atcatcacag caatagccac
     5401 accagtaatc acattgaaca tcatcgacat caaatgaacg ggtagcccat ccacgtgggc
     5461 ggtatcttca tcaaaagtca gaacatacat cggccgtttg aataaaaaga acaatatacc
     5521 gatggcaacg aataagattc ctaaaaagat gacttgctcc catgtaatgg tcacgatcga
     5581 accaaacaga taggattgga tacttgttgc tgaattccct ccgctcaaat tcatcaatac
     5641 taacgctagc gccaaaccac cagccattaa aatggcaatg gagatttctg aataggttcg
     5701 gtagatcgtt cgcagatact ccaatatcac agctgctaat acaacgacta gcatggtcgt
     5761 aagtgtagga ttaaggttca aaaagaatcc taacgctacc cctgctaaag agacatgaga
     5821 aagcgtatca gccatcagcg attgtcttct gatcactaaa aacacaccta gcatcggggc
     5881 gattccggca atgaagatag cggcaagaaa cgctcttctc atgaattcat atgaaagcaa
     5941 cgccattgag aatcctcctt tctcaccagt cggatctgtc tgtccgcata ctgtttgata
     6001 tcttcatgat catgggtgat cattagaact gatttcccat ggacatgcgc actatgtcgg
     6061 agaagacgat aaaattcatt tctggattct tcatccatac ctgttgttgg ttcatcaaga
     6121 acaaataagt cagggtcagt agcaaaaata cgcgctaaac tgattcgctg cttttgtccg
     6181 ccagacaatt cacctattcg ccgatttcgc atatcccaca tccctacagc ttctaaagca
     6241 cgctgtacat gttgatgatc tttttcagag agacgcttga accagcggcc tcttggatat
     6301 cttcctgaac ggactaattc gatcaccgta ctaggaaaac ctgcattgaa agatgcaact
     6361 tgttgaggaa tataaccgat acttaatttt tttccttctt gatttttctc agcaatcttt
     6421 atcattcctt ttgaaggttt cagtaatcct aacgatgctt tcaccaaggt tgacttggct
     6481 gctccatttt ctccagttaa aatgacaaat tcacccgcat ctacatgata agagacatct
     6541 tctagtacag gttcatcatc atagtaaaag gtcaaatcag atatttcgat ataacgcacc
     6601 ttacaacctc cattctaaaa cttaattgat actttctttt aaagctttca gatttttttc
     6661 catgacagaa atataatttt cgcctgcttt gatttgttca ttagtcaagc tttctaaagg
     6721 attcaacact tctagcttca ctcctgtttc attcgacaat gtctcagcta ccttcgaaga
     6781 ggcattttct tcaaaataaa tcactttgat gttgtttttc ttcacaaatt cttttagctc
     6841 agctaagcga cttggtgtgg gttcctgatc tggtgaaacg cccgaaatcg ctacttgttt
     6901 taagtcgtat tgattcgcta aataagcaaa agcagcatgc tgagtgacaa atgtgcggtt
     6961 tgttgcatct tttagctcat cggagtattt ttgatccagt gcttcaagct ttttgatata
     7021 agcatcggca ttcttcgtaa aagtttcctt tttatctgga tatttttcgc ctaattcttc
     7081 agcaatcgtt ctgacttctt ttatagctaa taccggatcg agccaaacat gtggatcgta
     7141 ttcatgtgaa tgaccatgcc cttcttctga ttcttcatgt tcgtctgctt cttgcgattc
     7201 taaacggtcg attcctttag ctgcttcgat catcaaggtc tgtttgctat ccactgaatc
     7261 ctttaacgaa tccacaaaaa attccatgtc tgaactatta tacacgaaaa catctgcatc
     7321 ggaaatttct gctctttctt ttgcacttgg ttcgaaatca tgcggctccg ttccagcagg
     7381 gatcaaaagt ttcacgtttc cttcatcccc tacgatctct tttgtaaaat catacatcgg
     7441 ataaaaagtc gtaacgatcg tcaaatcctc ttttttgtct gcttccttgt tcgtatttcc
     7501 acatgctgca aacagtaaag cacctatcat taccgtagct gtcaaaagat attttttcat
     7561 ttatatgctc ctttcaatga acaaatcgga attttacgat ttattaacgt gaactaatgt
     7621 atcaaaataa aaaaacacag tcaatagaaa tccatcaatt tctgatttaa gcatgtatat
     7681 tcttgattaa atcgtaaaat ggatgagttg ctgctcatat ctagccattt cactacaata
     7741 aaaaactgaa gaataccatt ttaggattcc tcagtttttc atcacctatt ttacttctta
     7801 tattttattg cagggttcgt actaaataaa cttcctcaca aaaacctttt aacccattat
     7861 atactctctg cgctcttgaa taatgttgac acaaggcaaa aacggttggt ccgcttccac
     7921 tcatcaatgc agcatctgct ccgtacttga tcatacgctc tttgatttga tggatgactg
     7981 gatgccgctt tcccgttata tcttctaaac tgtttcccat ttttttgatc atctgctgat
     8041 aatcttgctc tcggatggct tcagcaagtc cgtggatgtc ttgatgttgg atggcttgcg
     8101 tatctacttc tctaaacact gtacgtgtag agacactcat tcttggtttg accaacacaa
     8161 cccagcactg tggcatagaa ggtaatactt ggatttcttc ccctttgcca ccgacaaatg
     8221 ccgtacttcc gtagacacag taaggaacat ctgtaccgac ttccaaaccg atttctgcca
     8281 gttcttctaa cgaacagccc agattccata attggttgat ccctcttaga gcagctgcac
     8341 agtctgtact tcctccgcct aatccagctg ctactggaat attcttctta acaaagatct
     8401 tgacaccggt tttgatattg tatttatttt ttagcaataa agcagcttga taaacattgt
     8461 tccgttcatc taccggtaaa aatgctttat ttgtttctac aatgattttg tcttctgtta
     8521 tttcttcaaa aaatagatga tctgccaagt ctacgctcga catcaccatt tccaactcat
     8581 gatacccatc ttgccgtcta tataatacat ccagtcctaa atttatcttg gctggcgctt
     8641 tttcaatcaa ttccatcctc tcacccattc gaaaagtcta agtcgcaagt attctatcat
     8701 agggaaaaat aaaagtatag cttatgtctg cctagtggtt gattttgtca tcgtttcaac
     8761 agctttcaca gaattttctc ttcataatcc aaacaagttc tagtgattct tataattctg
     8821 tgagcacttc ttctgccaaa ctcggaacat ttcctttttc ttttcctatc agactgagta
     8881 gctgcatggt agcaaatccg aaattacttc ctttcctata agcaaaataa cgtccttccc
     8941 aatgatcgac atattttaat ttcttttcat aagcagactg gaaggaatag aaaaaattac
     9001 cataattata tacgatatgc atgacttttt cttgaaagaa agaaaaatct gtttctccaa
     9061 cattagctaa tggagctaag cctagattgg cttccgagta gcccattttc tcgtatacaa
     9121 taaacagatt ggctaacaga tagtcagcta attcctcaga ttctttaggt aagacccgca
     9181 acaaatcata tcccgcctgc ttcttcgtca tcggctgagc ggtgatgaag ccaacgactt
     9241 tttgatcttt acggacaata ccgacaccgc tagataatac atatgattta tcaaatcgtc
     9301 ccacagagaa gtatttttct cgtgcacctt gaagccattt ttgtgaaact tcatttacag
     9361 cttgatgtaa ttcttccggc agttgatcgt accaagtaaa atggtatcct tctttcatca
     9421 aacgttgata ttcaagttgc tgactcagcc gagacaagtc ttctgttgct agatcaacaa
     9481 tccctgcttc acctactttc gtgaagtgaa aacccaattc atgaagcatc actacgtagc
     9541 tttctgatat cttataaaac gcaagctgat agcctaaaca atctgcttgt ttcataaact
     9601 gattcgttgc tgcccgcatc attttaggat tgccaactgg atctcctaaa acaaagcatt
     9661 tgtttccttt gatctgataa ccaaaaacga cttgatcttg gtcgtcttct tggtaataaa
     9721 aataactgta acctgacaag cgaagctgat ggcttgtagc tgtgcctccg tatgtttcca
     9781 ataaacggtc aaaacggtct ttttcgtact cactgccaag cttcgtagta tcttcagcta
     9841 aatattgata aagcgccact aaagctagca atgagatgcc caaaccaata agccctgaga
     9901 accacacatc atctgaagga aacaaaataa aatgatttcc tgttattcga ccgactaaaa
     9961 agcgtccgct gtgatagcct gcaattgcgt aaacgataaa caaaaagccg aacaagctag
    10021 catcaaagat tatagctccc caagaataaa cgaattttgt tcgataaaat tcttttctgg
    10081 ataaccacac agctaacaga atcaagacat ataccaatat caactgccaa gataacgtac
    10141 gtgtgattgt atttaaaata ccaaaagata gtaaagcaat cgtaggcaga aatgcctttt
    10201 tgacttttac tgaaagtgct ctagcaaggc cgagtaataa aaagccaacc aatagattca
    10261 ctgtctgatc taaaaaattg aatgagaacg gcaataatac ttgaaatagc cgactgattg
    10321 cagacagatt agtgattgtt gatagaagta ccatcatgat ccccgcaaaa tagacagcac
    10381 ttacgagcac gatatgcgca accttttgga aaaacagtcg cggaatgtta tctaaaaatc
    10441 gattgatttt gactcctgta tgtgtgatga acaatagaag tcccgtcata aaaggtagaa
    10501 cataataaaa caaccgataa tataatagcc aaactactgc tacatcttgc cgaacagaca
    10561 attgactcat tcctaagatc atcaaaacgt caaaagtccc cattccacct gggaccatcg
    10621 tcagcattcc tatcaatgta gcaatcatga acataggata gatcgaaagc atcgaaatat
    10681 ctattctcat caacttgccg atgatcaaaa ataccgcaag ggctcccgtc cattgtccaa
    10741 aggatgccag tagcagacca agcaagcctt ttggataaaa ctcgaagaac aatgtctttc
    10801 tctttagata agcaaaaaag aaaatgactg gtgctaaaaa actaccgccg aaaagccaga
    10861 tccaatagct tcgaaaaata ctttctgact gaataaaaaa gacatcaata aatgtaagaa
    10921 acgaccatac agacaaacca gtcagcataa atagtgcgac ttttgataca gtggctaaaa
    10981 ccatttttct atttgtctgc tttccataaa aactagcccg cagacttgct cctaccacac
    11041 caccaaaacc ggcgagattg ttgatcgtgt ttgttaccca cgcagcaaga agaaactctt
    11101 ttctttccat ttttggtttt ccttgttttt ctaagactct gatcgtcacc caatcataca
    11161 acaacatcgg agtgacaccc agcagtccag ccagaatcat gagcaggatc gtcatgcggc
    11221 tttgttgtcc catcctttgc caaatatctt gccaactcat gccattagca atatttgcga
    11281 cttgatttgc cacaaagact aacacggagc cgaaaaaaat cagttttaaa atcagactat
    11341 gttcctttat ccattgcagc atcaagtatc gtttattttt cacattttcc cattcctaac
    11401 ttgtatactt ttgtgtcata ccccaatgtt atcttattta ttttaatttt tccactttag
    11461 acctgttctt tgcttgcgat gatagaaaaa gaaagatatc ctaatgctaa aggagaggat
    11521 aacatgaaag taggatttat cggcacaggg aatatggcac aagctattat tgatggttta
    11581 ttaaaaaaag gaacagtctc gaaagaagag gtgttcgttt ccagtggaca ttttgaaaat
    11641 gcacaaaaat ttgctgctga aactggcgtc catgcatgca aaagtaatca ggaagttgca
    11701 tcacaatcag atatcgttct tttagccgtg aaaccaaaaa tcattgaaaa agtattggaa
    11761 gaactaagag aatatagcca gaaagtctta ttcatttcta ttgcttcagg aaaatcatta
    11821 atagatttag aaacagcgct tgaagatcct caggcctcca tcgtacgtgt gatgccgaat
    11881 gtcaatgtca gcgtaggagc aggcgtttct gcgatttgca aaaatgaagc tgccacggag
    11941 gaacagcttc agcaagctac gatgattttt gaagcaatcg gcagcgtcta tcctttagca
    12001 gaatcagatt tcagtacatt cattggcctt gctggcagct cacccgcatt cgtttacatg
    12061 ctgatcgatg cgatgggacg gactggtgta ctgaatggtt tgtccaaaca gacagccact
    12121 gagattgctg caaaagcaat tatgggttct tgtcaaaaat tcctttcaag tgataaaaat
    12181 ccatgggaac ttgtagacca agtttcttct cctggcggta caactgttgc aggtgtagtt
    12241 gccttagaag aagcaggatt catcccagca atcatcaaag gaatcaatac gacgatcgag
    12301 aaagatcttg agatgttgaa taaaaaataa tgatagcaat gttttttcgt gccttacaaa
    12361 aaaagaagag ctgtggcttt tgccgcggct cttcttttgt attttttatt atttagcata
    12421 atcagttgca cgagtttccc gaataacgat tactttgatg tgtccaggat aatccagttc
    12481 ttcttcgatt cgtttacgaa tatcacgtac taaacgaaca gcgtctaaat ccgagatttc
    12541 ttctggcttg accattaccc gaacttcgcg tccagcttga actgcgtaac ttgattctac
    12601 accggcgaag ctgttcgaaa tattttccag acttcttaaa cgacggatgt agttttctaa
    12661 tgattcactt cgcgcaccag gtcttgctgc tgagagcgca tctgctgcag ctacaaggac
    12721 agagataaca gaagtagctt ccacatctcc atgatgagaa gcgatcgcgt tgattactac
    12781 tgggttttct ttgtacttgg cagctaattc cgcaccgatc tcaacgtgag atccttcaat
    12841 ctcatggtca agtgctttcc caatatcatg gagcaacccg gcacgttttg ctaattggat
    12901 atcttcgcca agctctgctg ctaagatacc agctaatttt gcaacctcga ccgaatgctt
    12961 caagacgttt tgtccataac tagttctgaa tcgcagacgt ccgatgatct tgattagatc
    13021 tggatgaagt gtatgagcac cgacttcaaa cgctgcctct tccccgtatt cgcgaatacg
    13081 ttcatccatt tctttacgag atttctcgac catttcttcg atacgcgctg gatggattcg
    13141 tccatcttgt atcagttttt ccaaggtcat ccgtgcgatt tctcttcgga ttggatcgaa
    13201 tccggaaaga acaactgctt ccggtgtatc atcgatgatt aaatcgatac cagtcagcgt
    13261 ctcaagcgtt cgaatattac gaccttctcg tccaatgata cgacctttca tttcatcatt
    13321 cggaagggta acgactgaaa cagtcgtttc tgaaacttga tctgctgcgc agcgttgaat
    13381 cgctaatgat aataagtttt tcgctttgcg atctgcttct tctttggcac gctgttcaga
    13441 ttcttttacc attagagtta actcatgatt cagctcttct tcagtcgatt tcatgatgac
    13501 atctcgtgcg tcctcttttg ataaaccagc aattttttct agttcttttt gttgatcttc
    13561 aatcattttt tcaacgtctt tttctcgctc ttcaattaat tgctgttttg aactaagtgc
    13621 ttcttcttta ctttcaagtg agttctcgcg tttttctaac gagtcatctt tacgattcag
    13681 cgtttgttcg cgttgaatca aacgattctc ttgagatttt aattctaact tactttctct
    13741 cagctcactt tcaacttcag aacggtattt ctgattttct tccttagctt ctaacaatgc
    13801 ttcttttttt agagtttctg cttctttttt tgcactatcg atgattccag cagctgatga
    13861 ttgtgcacca gctatttctt tctcgtgacg tgactttgcg accataaacc ctaaaccaag
    13921 accgacaatt aaaccgatga tagcgaggag aatgttgaat accataaaat ccacctccat
    13981 actatctttt tcatttttat catctataca gcttctatga taaattattc taaactcgtc
    14041 aatatgcatc ttcgcatata tgtgtacaaa aaacatgcac aactttattc taatgtttgt
    14101 accccgtagt gtcaacttta aataaacaaa gaaacggctt tctacttatt cattaaacga
    14161 tggtttttaa aataaatgac actagtttca tataggtaaa cctttaaatt gagaaacttt
    14221 tctattaaat tattctttgt agatgaacag atatttttat tttatgtacc cactcgtata
    14281 attaaataac tcacgatttc tttattcaat aaaaaaacgg caggtcaagg agagtaccta
    14341 ctcttcagac cagccgtttt tgtttatcat aatttttatt cttctactaa aggcaattct
    14401 tcttgtcctt tttcatcttc tgggacagcg acttcttcac caataccata agctgctcta
    14461 actagagcag aaacttctgc catcatttca ggatgattag ccatatagat tttagcgttt
    14521 tcccgtcctt gaccaatccg atcttcttta taagaatacc aagcaccgct tttatcaaca
    14581 atgtcttttt ctactgccat gtctagaagt tctccttctt gtgagatccc taaaccatac
    14641 atgacatcta cttcagcgat tttgaaagga ggtgctactt tgttttttac gactttgatc
    14701 tttgtacggt taccaacaat atctgtacct tgtttcaatt gttctgcacg acgtacttcc
    14761 aaacggatcg ttgcgtagaa tttcaatgcg cgtcctccag gagtgatttc tggattccca
    14821 aacatcacgc ctactttttc acggatttgg ttgatgaaaa tagcgattgt ttttgttttg
    14881 ttgatcgaac cagagagttt acgcaatgct tgagacatca aacgtgcttg taacccgaca
    14941 tgtgagtcac ccatttcgcc gtcgatttct gctcgtggaa ctaaagcagc aactgaatcg
    15001 actactacga tatctacagc cccacttgat actaaagcat cagcgatctc tagaccttgt
    15061 tctcctgtgt caggctgtga aagaagtagt tcatcgatat tcacacctaa tttttgtgca
    15121 tattgcggat ctaacgcatg ctcagcatca atgaaagcgg ccgttccgcc atttttttgt
    15181 acttctgcaa tagcgtgtag tgcaactgtt gttttacctg aactttcagg tccatatact
    15241 tcgatgatac gtccacgggg gtaaccgccg acacctaaag cgacatctaa cgcaagagag
    15301 ccacttggaa ttgtagagat ttgttggtcg attttttcgc ccaatttcat aatagagcct
    15361 ttaccatagc tcttttcaat ttttttcagt gcagcatcta aggctgcttt acgatcatct
    15421 gccaatacga taatcctcct tatatttaca aatcagagca acatttcatt atccttatca
    15481 tatctttttt agtgaaaaaa agcaagaaaa aaccgaacaa atattcgcat ttatcatttt
    15541 cttaaaagct gttttctcaa aagatcaaat ccttgcataa ccgcattata tctgatagca
    15601 gcacgatctc tggcaaagtg atatttttcc gaaaccacgc cgtctttaga tgaaatagcg
    15661 atccatactg ttcctgcaga ttgaccttct aacgtgtccg gtccagccac tccggtgaaa
    15721 gctacactgt aatctgttct tgctatctca cgtgcttgaa tggccatctg ctccacacat
    15781 tctttgctga ctgttccata ttctgctaat agctccggat caatctccag aaaaccagct
    15841 tttgtttgga gcgagtaggt aacgaatccg cctggaaata cttcagatac accagcaatg
    15901 tcgcctaatg cggcttggaa ggcgccggct gtcaaacttt ccgctgctgt tacagtctgc
    15961 ttgttttctt tcagtagttc cactacgact tttgctagcg aattatcatc gccgtatcca
    16021 taaaaatatt cccctactcg ttcttggatt ttttcttcta atgctaaaag agcttgattc
    16081 cctgcctgaa catcatttgt tttcactgtc agtcgtaatg tcacttcatt tggtttggca
    16141 tatggtgcga tcgttggatt gatctgtgtc tcaatcagat cttttagctc agtgaccagt
    16201 cgcgattcac caatcccata aaagcgcaaa actttagaaa ttagtttttc ttcagaagga
    16261 aaacgttcct ctagtaaagg acgtacttgt tccacaaaca ttggtttcaa ctcattaggc
    16321 ggaccgggaa gaaggatata cgcatgtgta tctgtttgat aaaaagtccc taaagcaagc
    16381 cctgtacgat ttggtagagg aaccccgcct tcgataatct gggactgttg cagattgttt
    16441 ttggtcattt ttcgatgggt tgtttcaaaa tatgccaata attttttgta tccttccgta
    16501 ttttgtatga gagacttacc gaggtgagcc gcagttgctt cttttgtcag gtcatcttct
    16561 gtcggtccta atccgccaca taagagtatg agttcacttc ttgtttcagc tagagcaatc
    16621 aactcttcta aacgctgctg attatctcct acaactgttt gataataaac ttcgatacct
    16681 agatcagcta gctgttctga caagaaagtg gcattcgtat tcacgacttg tcctagtaaa
    16741 agttctgttc cgacagctat tatttctgct ttcattactt cccgctccct tatatagtat
    16801 acgctgtatt tttatatttt tttatttaaa aagagggcaa ggagctgtga caaaaatctt
    16861 gtcacagctc cttgttccaa ataaacagtg ttctcctgcc aaaacctcgg tcataagtca
    16921 caatatacat aaaacatgaa aagctgtttt ttgaaattat cccttgtgac tcagagttag
    16981 acggcaggct cacaacctct ccactaaacg atacttacat tgatcctttg aacacaccgc
    17041 tgtttttgat gaagtaatcc acacctgaat agatagtaaa gatcagacaa gcataaagca
    17101 tgatttgatc tactggaata ccgatcaggc tgaatgggat attattgata aacagcaaga
    17161 taatagcaac catttgtgta gccgtcttga cttttcccgg ccaagctgca gccatgactt
    17221 ctccatgttc gacaagtagt aaacgcaagc ctgtgacggc taattcacga cagacaatga
    17281 tcgctactac ccaaccaggg gcctttcctt ggtcaaccaa catgataaaa gcagtcataa
    17341 ctagcatttt atctgccaac gggtccgcaa acttgccaaa attagtgaca aggcctcgcg
    17401 cacgggcaat ttttccatct aaccagtctg tgatactagc taaagcaaaa ataactgcac
    17461 ctactagctg tgtgacagcc agtgtggtgt ctcctacgtt aaattgtccc caatctaaag
    17521 gtaaacctac aaccaaaatg aaaatcggga tcatacaaat tctaagtaca gttaatttat
    17581 ttggtaaatt cacactatcg actcctttct tccctattct acgtgactat cagcgaagtt
    17641 tccaccgata ttatttactt ttccaaatga atttattcaa aggagtattt ttattgaaat
    17701 tgatcttttt gaattaacga agaaggaccg cgacacagat ttgtcacgat ccttcttttt
    17761 tatacctaca agtaaacaga tgcgtaaact aatttatacg atcattctgc gtattgaaga
    17821 gtaaggttca cattttttgg actctgattg ttttcccctg gattaactgg tacggattct
    17881 ccattcgctt tgatcgttgc attgcttgct gcaccaatcg tgatggtcgc atttgttgct
    17941 gtttccggta acacggtact ctgtgtttca tttgctgcca gtgtcccttg ataaacatag
    18001 gcattgtcta ctagcacacc taaccataca cgattgacag cagtaaattc aagggttaca
    18061 ggcttggtag cgtctgtgat atttactgcg atggcactgc cagaatcttc tcctgtagct
    18121 attgccattt tcttttcttc acttgatttt gttgttgatt ctactgtaga agactctttg
    18181 gtctgcgtgc tttgctctga ggaagaggac tcctcgactg atccttctac tttaagcgaa
    18241 gaagctgtgt ctccgattat tggatcagct tgacgatctt gccaagtcat atagcccacg
    18301 acaacgacaa tcgccaaagc aaccaatcct aataaaatga caggaagact tgtccaaaag
    18361 cgacttggat gcgattcttc ctgatgcatt gcttttctag aagtacttac tgtttcaggc
    18421 tgcggacgtt cagggaccga tcctgtcaat ttgcttttcc catcaaaaac atcgactaaa
    18481 tggtctccat cttctcctac agcttcagcg tattgccgaa taaatgcccg cacgtaaaat
    18541 ttaccaggca attggtcaaa atcatcattg tcgattgctt ccaaataacg tttttgaatt
    18601 tttgtaatct gctgtaattc atctaaagat atttttttat tcaggcgtgc ttgccgtaat
    18661 ttttctccga tggtttcaaa agccactcgt tcatcttctc cagtctgttt gattctccga
    18721 agcatctgct gttgcaacga aagacgttat gtttgggtgc tgtgacaaaa gtcatgtcac
    18781 agctcctttt ttccgaataa acattgttct cctgccaact cctcggtcac aagtcacaat
    18841 atccaataaa tataagaagc tgttttttga acttgttcct tgtgactcgg agttaaacgg
    18901 caggctcacg acctctttct taaacggtgt tcaatcagct gttcctcggt attagctcaa
    18961 aaaccggcaa aatataagga gatatttttc cgtatgtttc tctaatactc aggtcaaaac
    19021 gctgatttca caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagt
    19081 ccaaccaagt taaaaacatt aagaagctgt ttttacttgg ctgttcttat tacttgatgc
    19141 agggcagctt tttcacaacc tcttggtaca cggtgttcca gaagtagctt cttcggcaat
    19201 aagccgaaat atcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
    19261 agctgaccac ttctgtcaca acctctttga ttacttcgtt tgattttatt ttaatataag
    19321 ttgattaaaa gagagcatga tttgttttat tttctcacta atttttccaa cacaactaag
    19381 aataccataa aagctgattt tggaggaggt tcctactaca agtctaaaac gaaatttaag
    19441 acttttcctc ataaaatact ggtttatttt ggtctcatgt aaaactcgct gaaggcctct
    19501 tcacttataa atgcttcccc agctgccagt acgtctttca tttggatcga atcgatgatt
    19561 tcaggaagat caaataaagt tcgatcgcca aataaacttt gcgtaaattg attggcaata
    19621 tattcgatcg aatttaatga ttggaaatat tgtccaagca ttttcttctt cagcaaatcg
    19681 agatttgttt ctgacacttc cggatcatca gcgaatccaa gaatgatttc tttgactttt
    19741 tctgctgctt tctctggttc gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga
    19801 tccaagctga attcaaaacc aaagctgtca tcgataattc cttgattgta catcgctaga
    19861 tagtttcgag aagtattgcc taaaatcatt tggaacaaca gattgattgc tgttttatag
    19921 cgtaacagtt ctgttccttc ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat
    19981 ttatctcgtg taatggctgc ttccaatgca ctttgtttga tgatttcttt tgtgttttca
    20041 gggaaataac gtacaatttc ttgctttgga gggaaatttt ttgcttcttg gttttcccga
    20101 ataagtttca tcaatttctc aggctccatc ttaccaacta caaataaaac catattgctt
    20161 ggctgataaa acgtccgata gcacgtatac aagtcttgag ctgtgatttt gtcgatactt
    20221 tcaaccgtgc ccgcaatatc gatatgcaat gggtgtgtag gatacaagtt atttagtatg
    20281 ccaaaaaaca tccgccagtt agggtcgtct tcatacattt ggatttcctg accgatgatt
    20341 cctttttcct tgtttacagt ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga
    20401 gtcgtcagat tcttttctac ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa
    20461 cttgtgaaag cattggcgga agctccttgt ttcccaaata attgaaagac atcaccatct
    20521 tctttttcaa acaacttatg ttctaaaaaa tgggcaattc catctggaac tttcaccttt
    20581 tctttttcac catagggaat aaattcgtta tcgatcgaac cataattcgt actgaacaaa
    20641 ccatatgtct tgtgatagtc atttttaggc aacaaataca cggtcaaacc gtttggcagt
    20701 acttcatgat aaagtgtttc attgatttgc tcatattctg ttttattcat ttgcatttcc
    20761 tcctgctaaa aagaagattg cctgcaattc gatttgctca gctacttgtt gtatctcttt
    20821 gatcgttacg ctctccatac gtttcaacca ttcttcatca gataattttg tttccggcag
    20881 ccagctgtcc aaaaaagaag cttcgattgc cgcttgcgga gagtctagag aaagcagata
    20941 ttggtttctc agcattgctt ttgtctgaga cagttccaac tctgttattt caccatttcg
    21001 caaactttct aattgctcgt gaatcagatg aagtacttga tttcggttct tttcatcgat
    21061 accggtttgg acactcatga atcctcggaa cgtatccacg ctactagagg cataataagc
    21121 tagactttct ttctctctga cattcatgaa aagtttagag tgaggaaaac cgccaaataa
    21181 accgttaaag accattagcg caaaacgttc tggttcatcg taatacacat ttgtctgata
    21241 agctaggttc agctttgctt gaacgattgg ctcacgtact tgtcgttcct tcacgatatt
    21301 cacttgaggt tgggtataaa acagatctgg ataaactgtt tcccgcgact caaatggtaa
    21361 ttgcccaact gcttcagctg ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat
    21421 ttgatcattt gccatcattt cttgatagta ggacgctagc gaactagcag tgatcttgtc
    21481 caaatcttcg actacgccca accctgggat cttttgttcc gggctgtttt caaaataaag
    21541 ttcttgcaag gcataggaag caaaagtttg tttatcttcc ttcaaacttt ccaaatatag
    21601 acgcagattg ttcttttcca agtcaaacgt caactgatca aactgatgat ttttaatatt
    21661 tggataaaat aaaacctctt ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt
    21721 gacatattca ccattcacaa aactgatccc tgcgtttagc caatgtaaat ttcctttttt
    21781 tctaactgat aatccaaagc ttgcgccata aagctctgca agttgcgcac ttaactttgt
    21841 ctgatccgaa taattcaaac tatttgtttc caacatgctg ctgagaagtg aacgtttcgt
    21901 gatgacttct tgttgcagac gcgcagtaaa acggataaaa atccggatcg ttttgtattg
    21961 ttttgttggt aagacatgaa gattcacgcc ttctgccaat tgatacacca tatttatatg
    22021 ctccttttca cagttgattg cccgcttact cttttaatgg gtcaacctgc cttttttact
    22081 tattcctctg cttgaaattt ataagttcag gcaattcgcc gttttttcta aatgatgtta
    22141 cactttatag tatagcatag atgtttttta tcaaaagaaa cagacggcta aaagcgtata
    22201 ttgttttttt attttttatt tttcgatata ctaaagacaa ataaaagaag ggagggtcat
    22261 tcatgatcaa agaattcaaa gagttcatca tgcgtggaga tgtcttggat cttgctgtcg
    22321 gcgttgttat tggtagtgcg tttacaagca tcgtaaacca agtggtgcaa ggcttgatca
    22381 caccactaat aggttggttt gtcgcaatga tcacaggtac ttctgattta gaaggagcac
    22441 tttcaattct tgactggtcg ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta
    22501 gtgctatcat tacatttctg atcactggtt ttgtcttatt cttagttgtc aaagcggcaa
    22561 accgtgcgaa gaatttacgc tccaaagaag agcaagtgga agtggaagaa actcctactg
    22621 cagaagatta tcttagtgac atacgcgatc ttcttgccaa acaagtggca gaagacaata
    22681 cacatccagt agagcaaaca aatctttccg atacttctaa ataataaaga atctaaaaaa
    22741 aaagaaagcc gattttcagt gtcggctttc tttttttaga ttcttaactt gctacttgtt
    22801 cctttttcat gtcgatatgt ggaatatcat cttccaaata cacatctgag attgccttga
    22861 atccaaacga ctcataaaaa gcttgtaggt attcttgtgc gccgatcacg attggttttt
    22921 ctggaaaatt tttctggatc atgttaatcg tttcttgaac aatctttctt cctaaaccat
    22981 ctttacggtt gtcctgatga acaaggacac gtccaaaatg gatactctcc gcttcttcat
    23041 atactcgcgt gtaggcaagg atcttcccat cagtatcttg aaaaaacgta tgccaagctt
    23101 ctccatcaat gtcatcaact tcttgataag gacactgttg ttccacaaca aaaactgcga
    23161 ttcgttcttt gacgatcgcg taaaactctg ctgttgacaa ttcttcaaat ttctttacct
    23221 gatattccac taagcaaacg ccccctgctt ttttattctt agcataccga ttcgcacaca
    23281 aaaaagcaaa cgaaccttac gaatctcagg ctcgtttgct ttttaaactg cttcagttaa
    23341 aaattcgatc tcaactgtcc gagtaagtac atctgaatag ctgtaagata ctcgttcaaa
    23401 agaattttct tcaggatcca aatcaacaac gaatactgaa ggataggttt ctgttaaaac
    23461 gccgcggcgt tctgtttgac gctttcttcc tgtctgagca accaacatga ttgggctgcc
    23521 gatacgacct tctaaatctt tcttgattga agctaaagtt gttggcatac agttcacctc
    23581 tactatcaac tataccacat atttttaaaa ttttcaagtt taccataaaa aaaaaaagaa
    23641 atgcaataaa aataaataag aagctcatta agatttttct aaattattcc tcattataaa
    23701 atttcaagtg tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt
    23761 gatccattgc taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa
    23821 aaattttagc ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata
    23881 aaagatgctg aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt
    23941 ctgtctctat tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt
    24001 tctgcttcct caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa
    24061 agctaacacc tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt
    24121 cttgttccca atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta
    24181 aaagtggaaa atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag
    24241 cgcgcaataa ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct
    24301 tatcatcaat gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa
    24361 tcgtccaatt tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg
    24421 aaatcaataa aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc
    24481 gtcttatata attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt
    24541 tctgctaaat cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg
    24601 attctctcga tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg
    24661 tatttcttat aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact
    24721 tccattcctt catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc
    24781 gccattttgt cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag
    24841 ccgtctacaa tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc
    24901 tgatacataa gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc
    24961 gggatcgtca gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca
    25021 ttaccaatga ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc
    25081 atatctgtgc caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa
    25141 ttcgtcacgc gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata
    25201 ggtgtgattc caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct
    25261 gctgtccgta aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag
    25321 aaggggtctt cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca
    25381 taaggtgtaa tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat
    25441 ttttgtttag gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct
    25501 tctacttttt ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt
    25561 agcgcttcta ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt
    25621 tgatcagaaa ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt
    25681 ttgtcttcaa atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc
    25741 accaactttc tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa
    25801 atgcagatag cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc
    25861 attttttgcg ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc
    25921 ttctgtcaaa agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga
    25981 aacataattc gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag
    26041 gtggtcacga acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt
    26101 caattgtgtg taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct
    26161 aataagatcc ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga
    26221 ttgcttcttg cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct
    26281 tctgctgtaa ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt
    26341 gccaagacag cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc
    26401 tcatagacca ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat
    26461 cgctgttcta aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag
    26521 gcagtttctt ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg
    26581 gctgcttctt tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc
    26641 atgaagaaac gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa
    26701 ttccctagag atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc
    26761 atccagtaat tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca
    26821 tgatgaggaa attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt
    26881 tttgttgcca taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa
    26941 tcccaagaaa tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct
    27001 ttttgctgct gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat
    27061 tggtcactta atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa
    27121 gattcataag catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata
    27181 tgatccatga ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct
    27241 tcaaatgctc ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct
    27301 gtacggatga ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct
    27361 cgatattcaa aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata
    27421 tggatataat tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata
    27481 gggatgaatt cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc
    27541 tcactttttc tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt
    27601 ctggaacatc tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta
    27661 ccggtcctaa tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc
    27721 ttttacccgt ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt
    27781 cctcgatttc tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag
    27841 ttccaatcgt tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat
    27901 tgatcttcgc caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa
    27961 acaaggcatg aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat
    28021 cgtttctctt tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc
    28081 gtaagaattt tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag
    28141 tttccttatc aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc
    28201 tctgacagct aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt
    28261 cctgttttct gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat
    28321 accttgcacc acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc
    28381 gtgtttagtt cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga
    28441 tcgtttcagg caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga
    28501 aaagattttt tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag
    28561 ttggagcatc tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag
    28621 gaactgtttc accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga
    28681 aaaatagcgt agacagatca acgatttctg cagcatagct catttgaggt tgatacaggc
    28741 taacgatctt cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat
    28801 cagcgattag ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt
    28861 aatggttgtt tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc
    28921 gttttggatc gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag
    28981 gtgaccagcc caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc
    29041 gatattgttc gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt
    29101 cactgttgat gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat
    29161 agatcatcag ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga
    29221 ttttcatcaa atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt
    29281 tttggatgac aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca
    29341 tatctgtaaa tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac
    29401 cttgtgcttc tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat
    29461 gaggcatttc tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc
    29521 aatagcattt gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt
    29581 ctttacgttc agattgacgg taagggccgt attctccagg attttcagga gattcatccc
    29641 aattcattcc caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac
    29701 gtttttggtc agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca
    29761 aataattgaa taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg
    29821 catagcgtac gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa
    29881 cagcaaaaat tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac
    29941 aaattttacc ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg
    30001 gctgaatttc ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg
    30061 attcctcgtc ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg
    30121 gcacttgtta caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca
    30181 accatcgtgc cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact
    30241 actagaacat tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca
    30301 ttgatattaa gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg
    30361 gttacaatga caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg
    30421 atatcttcaa agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca
    30481 tttaggatat ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg
    30541 atgtattgta gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca
    30601 gtctttgtaa tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat
    30661 ttatggaaat tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt
    30721 tcttgctcga tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc
    30781 gttcccattc tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta
    30841 ttgacgaatg gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat
    30901 ccgatgatcg caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt
    30961 tgttctactt gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag
    31021 aaaataagtg cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt
    31081 tgaccgattg cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg
    31141 atcatcagca aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt
    31201 attagttagc ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg
    31261 attccttttg gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag
    31321 ccgagttttt ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca
    31381 gtcagtccaa tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt
    31441 gaagcaatac tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct
    31501 gcagctttta agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact
    31561 gccatgatca gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg
    31621 aacatagtag gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt
    31681 gccacaacga tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa
    31741 ggattagcga cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt
    31801 gagccaaatc ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca
    31861 aaatataaca ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct
    31921 ttggtaacat gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt
    31981 tctgcagtgg tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc
    32041 gtcatcgttt ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga
    32101 ctgatttcgt tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg
    32161 cgttccgcgc gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt
    32221 ccgcctatgg ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct
    32281 ggatcgccac caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc
    32341 agttcttcta attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt
    32401 gttggttttg ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt
    32461 tcgatttctt cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta
    32521 ggcgacacat acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta
    32581 ccgcctcctt ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt
    32641 atttactgga agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt
    32701 ttaaaaacgg caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct
    32761 tgaaaccaaa gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat
    32821 aatcactgtc aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc
    32881 ttgaacggtt agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag
    32941 gcacgagggt tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg
    33001 cgactgtttc caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt
    33061 ctgctgcttc aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat
    33121 tttgatcttt atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct
    33181 aaataaacta ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct
    33241 tcaagaaatt ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca
    33301 agttgtacaa aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac
    33361 gaaattaatg agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa
    33421 aacgaattat tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc
    33481 ttgcggcaat cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag
    33541 tgatcttagt ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac
    33601 gtccgacttt gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga
    33661 atatctataa tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa
    33721 gagacgtggt agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt
    33781 tgattatgga cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag
    33841 atcatctgac aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg
    33901 cggcagacct tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta
    33961 caaatcccga ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac
    34021 aagcaggtgg tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg
    34081 cttctaggat atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa
    34141 ttatttttga tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat
    34201 gactgacttg atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat
    34261 gcctacaaac cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca
    34321 acaagcgatc attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag
    34381 tgaaacgatg caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga
    34441 gatcgaaaaa attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa
    34501 caccgacgga ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta
    34561 cgaatctcgt ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc
    34621 cgttatcctt cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt
    34681 gcagcaagta ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac
    34741 ttctcatgaa actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc
    34801 tcgaggcgga gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga
    34861 aacaggtaca ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt
    34921 gaacatcatc gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt
    34981 gctgatccat tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga
    35041 acacggggtc tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt
    35101 agcagaagac acagattggg atactgaata tctagattat atccttgctg tcaaagtggt
    35161 cgattctttg gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac
    35221 gatcgtgaca gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc
    35281 tgtctatgtc aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga
    35341 gattggtatc tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac
    35401 ttccacaaaa tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa
    35461 cattattccc gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa
    35521 tatgtttgaa attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt
    35581 tctgggagtc ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca
    35641 acgggaaaaa tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt
    35701 gcacattagt tacatggttg cattatgctt attagcgaca atcaattact attctttttt
    35761 cgatcattca atcgcttata cgattacgct actgattgct gggatttttc acttatctta
    35821 taaatatcag ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg
    35881 ataagtagag tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa
    35941 agattttgtc acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa
    36001 atctgtttcc tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg
    36061 aagcagctca tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc
    36121 cagtagctgt tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct
    36181 agtttcacct tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg
    36241 atcatctgaa aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc
    36301 ttccagcatc tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa
    36361 aattttccag tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc
    36421 ccctttttcc gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc
    36481 aacaaacatg agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt
    36541 ctcacaacct cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac
    36601 ggtaaaatat gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg
    36661 attttacaac ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa
    36721 cggcaaaata tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg
    36781 attttacaac ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac
    36841 caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca
    36901 gctttttcac aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga
    36961 taaatgatac aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt
    37021 tcaccaatat gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag
    37081 cggacaaaac tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca
    37141 atcgtcgtca acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc
    37201 ggtacaagga ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag
    37261 acaaatgctg ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg
    37321 aaaatcgaag caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat
    37381 tcagaatagc caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta
    37441 tataacaatg cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct
    37501 tcccaaaggc ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct
    37561 ataaaaccgc aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg
    37621 cccaagaccc caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac
    37681 atcatgattg ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta
    37741 gagacaaata aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata
    37801 ttcccagata atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat
    37861 gtttttttca aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg
    37921 actaacatgt aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca
    37981 gcaatcgttt ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta
    38041 gctgctacaa ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata
    38101 tctatttgat tctccaatat ttccatccct ttctctctat tagcatcata catgatttat
    38161 tttacatgtc aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa
    38221 taagacgaga ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt
    38281 atccaaacca cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca
    38341 tcacagtcag atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt
    38401 ggacaaagac gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct
    38461 tgtagaggaa tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc
    38521 aaaaatctat gctttttcga cgctcgataa aacaacagct actggactgc tattcactag
    38581 agaacaacag ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct
    38641 agacaagaaa caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca
    38701 gcaggaactc caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg
    38761 gataaacaga acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc
    38821 aatacagttg gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca
    38881 ctatttttct agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc
    38941 aaataacagt gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg
    39001 ggccaacgat gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca
    39061 tataagcaga gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca
    39121 tacatgtcac cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc
    39181 tttctggatt tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga
    39241 catccaaaat gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat
    39301 gaagatcttg atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca
    39361 tcattgttgt aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta
    39421 aatttgtcac gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt
    39481 ttctgccctt ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga
    39541 gaagtaaaca aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt
    39601 gccatatcaa taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa
    39661 agcaaagcaa tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc
    39721 aaagtttcct gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac
    39781 tctgtttgtc tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt
    39841 tgctgatata cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg
    39901 atatgttcta ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta
    39961 cctaaagaaa actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg
    40021 acattttgtc ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa
    40081 cggatttgtt tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct
    40141 aatgttttcg cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac
    40201 aaccgatcca gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc
    40261 gcttgttttt tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac
    40321 tctgcttctc gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa
    40381 ttccccgaaa actctcggat ttctccgtct tttacttccc agattttcga tgctagctca
    40441 tctagaaaat agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct
    40501 tcaatcagtc gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc
    40561 ccttgttcat aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc
    40621 catttccgct ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt
    40681 aggtcctttg cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt
    40741 tggattttcc catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta
    40801 ccgacaccat tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca
    40861 atagatagtt ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa
    40921 ttttccatat gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag
    40981 agcagaaaaa tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata
    41041 actatgacta aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa
    41101 atggacatac tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg
    41161 ttattcaaaa gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt
    41221 attaggttac cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct
    41281 tttattcgaa catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta
    41341 ccgcttttcc tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg
    41401 cctctgccaa ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac
    41461 gcgcaactaa catgaattgc atagccgcta agctagaatc gactttgact acatcgttca
    41521 tttcttctct tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga
    41581 cttcttttgg catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt
    41641 aacattgcat atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat
    41701 tgaaacggat caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc
    41761 gccatggttt catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca
    41821 tttcttcgtg tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca
    41881 taattgttga aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt
    41941 atattgcaaa gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca
    42001 tcgctgaaag ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg
    42061 attttcttct gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt
    42121 ctctgacgcg aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct
    42181 tgatcaatct gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt
    42241 ctcatcatga tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg
    42301 attttttgat cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata
    42361 atttcctcct ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa
    42421 aagaaaaaag tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata
    42481 gagaaaagaa attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa
    42541 accaagatta ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt
    42601 acaccgccaa aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg
    42661 gatcagtatg cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt
    42721 gagtaaacgg tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat
    42781 gaaaatctgt ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc
    42841 tctttttaat aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa
    42901 tctctttcct tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat
    42961 agtcgataaa ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg
    43021 atttttgtta aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa
    43081 ggattgtata aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca
    43141 tattcccagc ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt
    43201 tctcctgtat gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca
    43261 tactgtgtca gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca
    43321 gtggcataac gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc
    43381 catgcaccgg aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg
    43441 ttatcttgca atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc
    43501 cattgcccat tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct
    43561 ttaatcccaa atagattgta attaggggca gaagccaaag tactgttccc ccagccactt
    43621 tcaacgatgg cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt
    43681 tgtgcagttg aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca
    43741 taaaaagcat agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc
    43801 aatgtttgcc catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga
    43861 gcttctgctg atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct
    43921 tcggttgaaa ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt
    43981 tcaattcctg tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca
    44041 gctagcactg ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa
    44101 ttctttgctt tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta
    44161 actaaaaaag taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata
    44221 aatttgcttt ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt
    44281 aaattgatgg tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta
    44341 tggattatac ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg
    44401 gcgtttctat cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt
    44461 tttgcgcttc atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag
    44521 tttgaacagc tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac
    44581 gtaatccttc catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag
    44641 gataaacaac atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt
    44701 ccatgattgg aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca
    44761 tattttgcca ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat
    44821 tattgtacaa tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga
    44881 cttgtgcaaa atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt
    44941 cttttattgc ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt
    45001 ctgctaatag cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca
    45061 ctttttctga taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata
    45121 ctcactatct cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt
    45181 tggcagggat tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg
    45241 ctgcaacaat ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga
    45301 tccaagcatt tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat
    45361 cggttagatg gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta
    45421 aacagatgcc acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta
    45481 tactaatatg tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta
    45541 ctttgtcact aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag
    45601 tattcagcgc tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga
    45661 gcggatccgt tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt
    45721 tgctccctcc ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa
    45781 acatacttaa ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg
    45841 aaaaaatgga tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca
    45901 gccgagcagc aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt
    45961 tagaagaaga acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg
    46021 attctggtcg atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg
    46081 cgaacaattt aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa
    46141 ctaaagcaat ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata
    46201 tccagtttca tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat
    46261 tacttgattt tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac
    46321 tgccagccca agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa
    46381 cagtcatcca tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag
    46441 tggacagtca actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa
    46501 catataactt attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag
    46561 ctctagatgg gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc
    46621 cattacaagc ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc
    46681 gtaaattttt agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt
    46741 tcagcttttt aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt
    46801 cttaatttgt tccttccttt tatttctgat aattccagta aattactata attataaaaa
    46861 aagaattgac aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc
    46921 atgtgactag atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt
    46981 ttttcgtgtc tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat
    47041 cttcaaaaag ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt
    47101 agatgacggt ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag
    47161 aaatgatttg atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca
    47221 aatcaagttg caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac
    47281 gatcattgat catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc
    47341 tttgaccgac cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa
    47401 caaactttta agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc
    47461 agtagagtat ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat
    47521 cgcaatccat attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga
    47581 agtgacgatt gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca
    47641 ttcagaacca ttttcactga attattcaag actggtgaat catttacgac tgcttctaca
    47701 acgaacccat gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg
    47761 aaaataccca gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata
    47821 ccagctcgcg atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag
    47881 aagcgcaatc gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat
    47941 ctacaacgaa tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta
    48001 ctagtaggga tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta
    48061 caagctggcg gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca
    48121 ctagggatgt cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa
    48181 gttccagtaa atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa
    48241 tcagttgatc catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg
    48301 attgcggcag ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc
    48361 tttagcggca agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca
    48421 gtgctactgc tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt
    48481 ttgagtttag gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct
    48541 acaggattgc atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat
    48601 attggtaaat ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga
    48661 tttttcccaa tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca
    48721 cgtccagaaa acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc
    48781 tttactggtg taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat
    48841 gtcttgcatg caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca
    48901 gcaggatttg cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt
    48961 gcgaatcagc cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt
    49021 ggctttaact ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat
    49081 ctttcagatg acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc
    49141 aaaatctacg ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca
    49201 cgccttcgtt tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact
    49261 ggcgtacctg gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa
    49321 gtacaattcg ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca
    49381 caaacaaaca ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt
    49441 tacgctatcg caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg
    49501 gaaaaaatga tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca
    49561 atcgctggta cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat
    49621 gctggtatcg aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca
    49681 ttcactttat atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt
    49741 gatcttgccg ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat
    49801 ggcgacaaag tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa
    49861 atcggctctg tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa
    49921 acaaacagga gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt
    49981 tctcctgcca actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt
    50041 tatttgactc ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt
    50101 gttcaaaaac cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt
    50161 tcacaaaatt tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt
    50221 attcctgatc caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata
    50281 ctttttctaa atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg
    50341 ctaaaaacat gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg
    50401 ctgcatcact tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg
    50461 ctaattcttc atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt
    50521 ctactagtac ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc
    50581 catttcctgc agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt
    50641 cattctctac ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca
    50701 ttttttcagt gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa
    50761 aacttgtata agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat
    50821 gttcggcttg acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca
    50881 acgcttcttt cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt
    50941 ctttcagtgc ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa
    51001 ccatcttttt taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta
    51061 aataatgcat atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt
    51121 tataaattac acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa
    51181 ggatgacaac tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat
    51241 attgaaatgt ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa
    51301 acaaatgcag caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt
    51361 acaaaaaccg gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta
    51421 gatgtctatc gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat
    51481 ccaaattgcg tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg
    51541 cagaaaaagg cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata
    51601 gccgttttgg aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa
    51661 ttctctctac tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct
    51721 ccgggggaca aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct
    51781 gaagtcatga cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa
    51841 aaatgcaaaa tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc
    51901 tactttcacg acctctatat tatcatggtt tattgactac cggtatccag atttctccca
    51961 tatatttccc gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt
    52021 aatcttctac ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt
    52081 cttcaggcga ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga
    52141 ttcgggtagt gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc
    52201 cgttataggg ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat
    52261 ctattgttcc attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga
    52321 caaaatccgt attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt
    52381 tttcttcgat cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt
    52441 acataaaatt aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt
    52501 ttaactattt ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct
    52561 ctccccattt tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa
    52621 gccattctgc atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat
    52681 taggaaaaat ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat
    52741 tttcttttgt cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg
    52801 ctccatcaaa tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat
    52861 gcatctcttc cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga
    52921 ctccttcaaa tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct
    52981 tttcgatacg aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg
    53041 gaaaagattt ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac
    53101 gaaaagctct tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca
    53161 ctcttgatcc atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt
    53221 ccattagcga catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc
    53281 ctgtattttc ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat
    53341 cgatcaattg gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca
    53401 tattatcaaa gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa
    53461 atgttgtatg gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta
    53521 tctgcctatc tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta
    53581 aacaaagcgg taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca
    53641 aagcaaattg ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca
    53701 tcatacctct tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg
    53761 tcagataaat aaaa
//
LOCUS       DAZKBH010000006        52138 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000006 DAZKBH010000000
VERSION     DAZKBH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.006"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACG8OO_000386"
     CDS             290..1135
                     /locus_tag="ACG8OO_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9044324.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="ACG8OO_000387"
     CDS             1416..2636
                     /locus_tag="ACG8OO_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9044325.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="ACG8OO_000388"
     CDS             2633..5353
                     /locus_tag="ACG8OO_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9044326.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="ACG8OO_000389"
     CDS             5337..6281
                     /locus_tag="ACG8OO_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9044327.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="ACG8OO_000390"
     CDS             complement(6350..7360)
                     /locus_tag="ACG8OO_000390"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9044328.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="ACG8OO_000391"
     CDS             complement(7540..7884)
                     /locus_tag="ACG8OO_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9044329.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="ACG8OO_000392"
     CDS             complement(7877..8311)
                     /locus_tag="ACG8OO_000392"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFP9044330.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="ACG8OO_000393"
     CDS             8449..9186
                     /locus_tag="ACG8OO_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044331.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="ACG8OO_000394"
     CDS             9179..10390
                     /locus_tag="ACG8OO_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9044332.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="ACG8OO_000395"
     CDS             10594..11376
                     /locus_tag="ACG8OO_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFP9044333.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="ACG8OO_000396"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="ACG8OO_000396"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9044334.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="ACG8OO_000397"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="ACG8OO_000397"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9044335.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="comGA"
                     /locus_tag="ACG8OO_000398"
     CDS             14092..15021
                     /gene="comGA"
                     /locus_tag="ACG8OO_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFP9044336.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /gene="comGB"
                     /locus_tag="ACG8OO_000399"
     CDS             14978..16027
                     /gene="comGB"
                     /locus_tag="ACG8OO_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFP9044337.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /gene="comGC"
                     /locus_tag="ACG8OO_000400"
     CDS             16024..16302
                     /gene="comGC"
                     /locus_tag="ACG8OO_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFP9044338.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /gene="comGD"
                     /locus_tag="ACG8OO_000401"
     CDS             16277..16747
                     /gene="comGD"
                     /locus_tag="ACG8OO_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFP9044339.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16719..17066
                     /locus_tag="ACG8OO_000402"
     CDS             16719..17066
                     /locus_tag="ACG8OO_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044340.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            17029..17454
                     /gene="comGF"
                     /locus_tag="ACG8OO_000403"
     CDS             17029..17454
                     /gene="comGF"
                     /locus_tag="ACG8OO_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFP9044341.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /gene="comGG"
                     /locus_tag="ACG8OO_000404"
     CDS             17426..17785
                     /gene="comGG"
                     /locus_tag="ACG8OO_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFP9044342.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="ACG8OO_000405"
     CDS             17957..18964
                     /locus_tag="ACG8OO_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9044343.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="ACG8OO_000406"
     CDS             18989..20173
                     /locus_tag="ACG8OO_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFP9044344.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /gene="abc-f"
                     /locus_tag="ACG8OO_000407"
     CDS             complement(20610..22193)
                     /gene="abc-f"
                     /locus_tag="ACG8OO_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFP9044345.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22298..22426)
                     /locus_tag="ACG8OO_000408"
     CDS             complement(22298..22426)
                     /locus_tag="ACG8OO_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044346.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22771..22914)
                     /locus_tag="ACG8OO_000409"
     CDS             complement(22771..22914)
                     /locus_tag="ACG8OO_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9044347.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23136..23615)
                     /locus_tag="ACG8OO_000410"
     CDS             complement(23136..23615)
                     /locus_tag="ACG8OO_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9044348.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23688..23972)
                     /locus_tag="ACG8OO_000411"
     CDS             complement(23688..23972)
                     /locus_tag="ACG8OO_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044349.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24050..24244)
                     /gene="ssrS"
                     /locus_tag="ACG8OO_000412"
     ncRNA           complement(24050..24244)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8OO_000412"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24316..25602)
                     /locus_tag="ACG8OO_000413"
     CDS             complement(24316..25602)
                     /locus_tag="ACG8OO_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFP9044350.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25740..26411
                     /locus_tag="ACG8OO_000414"
     CDS             25740..26411
                     /locus_tag="ACG8OO_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFP9044351.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26377..26865
                     /locus_tag="ACG8OO_000415"
     CDS             26377..26865
                     /locus_tag="ACG8OO_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFP9044352.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26878..27825
                     /gene="prmA"
                     /locus_tag="ACG8OO_000416"
     CDS             26878..27825
                     /gene="prmA"
                     /locus_tag="ACG8OO_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9044353.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27826..28590
                     /locus_tag="ACG8OO_000417"
     CDS             27826..28590
                     /locus_tag="ACG8OO_000417"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9044354.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28823..31036
                     /locus_tag="ACG8OO_000418"
     CDS             28823..31036
                     /locus_tag="ACG8OO_000418"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9044355.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31047..31493
                     /gene="dtd"
                     /locus_tag="ACG8OO_000419"
     CDS             31047..31493
                     /gene="dtd"
                     /locus_tag="ACG8OO_000419"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9044356.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31664..32923
                     /locus_tag="ACG8OO_000420"
     CDS             31664..32923
                     /locus_tag="ACG8OO_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFP9044357.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33012..33959)
                     /locus_tag="ACG8OO_000421"
     CDS             complement(33012..33959)
                     /locus_tag="ACG8OO_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9044358.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34353..35645
                     /gene="hisS"
                     /locus_tag="ACG8OO_000422"
     CDS             34353..35645
                     /gene="hisS"
                     /locus_tag="ACG8OO_000422"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9044359.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35660..37429
                     /gene="aspS"
                     /locus_tag="ACG8OO_000423"
     CDS             35660..37429
                     /gene="aspS"
                     /locus_tag="ACG8OO_000423"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9044360.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37538..38413
                     /locus_tag="ACG8OO_000424"
     CDS             37538..38413
                     /locus_tag="ACG8OO_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9044361.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38679..40562
                     /locus_tag="ACG8OO_000425"
     CDS             38679..40562
                     /locus_tag="ACG8OO_000425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9044362.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40637..40921)
                     /locus_tag="ACG8OO_000426"
     CDS             complement(40637..40921)
                     /locus_tag="ACG8OO_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9044363.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40911..41645)
                     /locus_tag="ACG8OO_000427"
     CDS             complement(40911..41645)
                     /locus_tag="ACG8OO_000427"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFP9044364.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41774..42397
                     /locus_tag="ACG8OO_000428"
     CDS             41774..42397
                     /locus_tag="ACG8OO_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP9044365.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42589..43710
                     /locus_tag="ACG8OO_000429"
     CDS             42589..43710
                     /locus_tag="ACG8OO_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9044366.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43717..46845
                     /locus_tag="ACG8OO_000430"
     CDS             43717..46845
                     /locus_tag="ACG8OO_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFP9044367.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46864..50058
                     /locus_tag="ACG8OO_000431"
     CDS             46864..50058
                     /locus_tag="ACG8OO_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFP9044368.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50204..50911)
                     /locus_tag="ACG8OO_000432"
     CDS             complement(50204..50911)
                     /locus_tag="ACG8OO_000432"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9044369.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51676..52074
                     /gene="spxA"
                     /locus_tag="ACG8OO_000433"
     CDS             51676..52074
                     /gene="spxA"
                     /locus_tag="ACG8OO_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFP9044370.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17109 a         9065 c        10790 g        15174 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 ttgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cgggacagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acagtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatat cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac
    23041 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
    23101 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
    23161 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
    23221 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
    23281 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
    23341 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
    23401 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
    23461 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
    23521 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
    23581 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
    23641 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
    23701 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
    23761 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
    23821 aaaacagcat atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg
    23881 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
    23941 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
    24001 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
    24061 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
    24121 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
    24181 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
    24241 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
    24301 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
    24361 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
    24421 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
    24481 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
    24541 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
    24601 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
    24661 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
    24721 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
    24781 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
    24841 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
    24901 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
    24961 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
    25021 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
    25081 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
    25141 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
    25201 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
    25261 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
    25321 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
    25381 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
    25441 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
    25501 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
    25561 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
    25621 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
    25681 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
    25741 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
    25801 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
    25861 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
    25921 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
    25981 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
    26041 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
    26101 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
    26161 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
    26221 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
    26281 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
    26341 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
    26401 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
    26461 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
    26521 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
    26581 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
    26641 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
    26701 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
    26761 gaagctgtat tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa
    26821 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
    26881 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
    26941 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
    27001 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
    27061 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
    27121 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
    27181 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
    27241 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
    27301 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
    27361 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
    27421 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
    27481 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
    27541 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
    27601 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
    27661 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
    27721 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
    27781 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
    27841 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
    27901 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
    27961 atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt
    28021 aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
    28081 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
    28141 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
    28201 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
    28261 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
    28321 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
    28381 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
    28441 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
    28501 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
    28561 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
    28621 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
    28681 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
    28741 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
    28801 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
    28861 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
    28921 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
    28981 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
    29041 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
    29101 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
    29161 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
    29221 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
    29281 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
    29341 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
    29401 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
    29461 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
    29521 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
    29581 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
    29641 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
    29701 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
    29761 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
    29821 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
    29881 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
    29941 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
    30001 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
    30061 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
    30121 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
    30181 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
    30241 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
    30301 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
    30361 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
    30421 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
    30481 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
    30541 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
    30601 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
    30661 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
    30721 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
    30781 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
    30841 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
    30901 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
    30961 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
    31021 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
    31081 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
    31141 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
    31201 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
    31261 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
    31321 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
    31381 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
    31441 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
    31501 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
    31561 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
    31621 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
    31681 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
    31741 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
    31801 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
    31861 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
    31921 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
    31981 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
    32041 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
    32101 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
    32161 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
    32221 gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
    32281 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
    32341 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
    32401 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
    32461 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
    32521 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
    32581 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
    32641 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
    32701 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
    32761 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
    32821 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
    32881 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
    32941 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
    33001 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    33061 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    33121 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    33181 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    33241 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    33301 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    33361 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    33421 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    33481 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    33541 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    33601 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    33661 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    33721 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    33781 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    33841 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    33901 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    33961 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    34021 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    34081 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    34141 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    34201 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    34261 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    34321 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    34381 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    34441 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    34501 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    34561 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    34621 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    34681 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    34741 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    34801 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    34861 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    34921 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    34981 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    35041 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    35101 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    35161 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    35221 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    35281 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    35341 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    35401 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    35461 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    35521 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    35581 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    35641 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    35701 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    35761 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    35821 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    35881 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    35941 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    36001 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    36061 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    36121 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    36181 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    36241 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    36301 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    36361 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    36421 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    36481 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    36541 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    36601 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    36661 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    36721 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    36781 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    36841 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    36901 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    36961 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    37021 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    37081 ttatgatatc gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg
    37141 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    37201 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    37261 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    37321 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    37381 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    37441 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    37501 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    37561 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    37621 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    37681 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    37741 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    37801 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    37861 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    37921 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    37981 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    38041 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    38101 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    38161 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    38221 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    38281 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    38341 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    38401 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    38461 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    38521 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    38581 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    38641 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    38701 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    38761 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    38821 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    38881 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    38941 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    39001 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    39061 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    39121 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    39181 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    39241 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    39301 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    39361 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    39421 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    39481 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    39541 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    39601 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    39661 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    39721 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    39781 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    39841 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    39901 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    39961 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    40021 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    40081 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    40141 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    40201 aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag
    40261 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    40321 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    40381 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    40441 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    40501 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    40561 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    40621 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    40681 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    40741 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    40801 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    40861 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    40921 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    40981 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    41041 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    41101 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    41161 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    41221 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    41281 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    41341 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    41401 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    41461 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    41521 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    41581 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    41641 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    41701 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    41761 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    41821 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    41881 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    41941 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    42001 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    42061 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    42121 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    42181 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    42241 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    42301 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    42361 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    42421 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    42481 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    42541 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    42601 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    42661 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    42721 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    42781 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    42841 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    42901 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    42961 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    43021 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    43081 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    43141 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    43201 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    43261 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    43321 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    43381 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    43441 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    43501 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    43561 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    43621 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    43681 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    43741 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    43801 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    43861 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    43921 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    43981 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    44041 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    44101 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    44161 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    44221 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    44281 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    44341 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    44401 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    44461 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    44521 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    44581 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    44641 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    44701 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    44761 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    44821 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    44881 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    44941 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    45001 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    45061 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    45121 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    45181 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    45241 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    45301 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    45361 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    45421 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    45481 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    45541 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    45601 agcaagatgg ccaatgaaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    45661 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    45721 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    45781 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    45841 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    45901 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    45961 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    46021 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    46081 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    46141 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    46201 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    46261 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    46321 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    46381 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    46441 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    46501 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
    46561 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    46621 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    46681 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    46741 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    46801 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    46861 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    46921 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    46981 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    47041 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    47101 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    47161 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    47221 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    47281 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    47341 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    47401 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    47461 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    47521 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    47581 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    47641 caaaacgatc aaggaataca attttctgat caaaacaaac ctttatcatt caaagtggaa
    47701 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    47761 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    47821 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    47881 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    47941 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    48001 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc
    48061 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    48121 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    48181 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    48241 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    48301 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    48361 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    48421 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    48481 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    48541 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    48601 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    48661 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    48721 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    48781 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    48841 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    48901 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    48961 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    49021 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    49081 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    49141 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    49201 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    49261 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    49321 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    49381 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    49441 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    49501 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    49561 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    49621 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    49681 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    49741 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    49801 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    49861 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    49921 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    49981 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    50041 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    50101 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    50161 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    50221 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    50281 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    50341 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    50401 ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    50461 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    50521 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    50581 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    50641 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    50701 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    50761 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    50821 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    50881 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    50941 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    51001 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    51061 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    51121 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt
    51181 taatacagtc tcaatataac tattcaaaca taaaataacg ttatttgatt tatttgttta
    51241 tagtaaattt tatttagtaa tcattgcttt aaaatactac gcttatataa cagagaaaat
    51301 aagcgttata ataaagaaca gtattattta aaatgacaga aaacattact attaaagaaa
    51361 taaaaaacat aaaaaatctt aagaaatcgg tttcatttag cttttgagat aaaataatag
    51421 tacagtttta tacgctgttt catatgttta gaaaatttga attagatttg aaaactctaa
    51481 tttttaaatg ataaaaaaag cgagaatgct ggttttactg tatattaacc ttagataact
    51541 ttcatttttt taaaaaaatt atgaaaaaaa ggtagctatt taaaagttga ttatgtataa
    51601 tagaaacaga tgggagatta ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa
    51661 aaggagtggt gcacgttgtt aacattatat acttccccaa gttgtacttc ctgcagaaag
    51721 gctcgtgcgt ggttgcaaga acatcagatt ccatttgttg aacgtaatat tttttcagaa
    51781 ccattgaaca gttctgaatt aaaagcgatt ttacaaatga cagaagacgg aacagaagaa
    51841 atcatctcga cacgttccaa agtattccaa aaactgaaca tggatttaga tgatctgccg
    51901 ttacaagaat tattggaact tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg
    51961 attgatgata aacgtctgca agttggtttt aatgaagatg aaattcgtcg gttcctaccg
    52021 cgcgatgtac gacaattaga attacgtcaa gcacaattga tggctggatt atagaaggat
    52081 aatacgaaac ttggaaaaga ctggctcttt gtcaaatgat gttggtaaag agaaatct
//
LOCUS       DAZKBH010000007        51740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000007 DAZKBH010000000
VERSION     DAZKBH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.007"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            344..3151
                     /locus_tag="ACG8OO_000434"
     CDS             344..3151
                     /locus_tag="ACG8OO_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9044371.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3342..3833
                     /locus_tag="ACG8OO_000435"
     CDS             3342..3833
                     /locus_tag="ACG8OO_000435"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9044372.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3936..4925
                     /locus_tag="ACG8OO_000436"
     CDS             3936..4925
                     /locus_tag="ACG8OO_000436"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9044373.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4958..5761
                     /locus_tag="ACG8OO_000437"
     CDS             4958..5761
                     /locus_tag="ACG8OO_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP9044374.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5782..6696
                     /locus_tag="ACG8OO_000438"
     CDS             5782..6696
                     /locus_tag="ACG8OO_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9044375.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6779..7153
                     /locus_tag="ACG8OO_000439"
     CDS             6779..7153
                     /locus_tag="ACG8OO_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFP9044376.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7292..7537
                     /locus_tag="ACG8OO_000440"
     CDS             7292..7537
                     /locus_tag="ACG8OO_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9044377.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7709..8773
                     /locus_tag="ACG8OO_000441"
     CDS             7709..8773
                     /locus_tag="ACG8OO_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFP9044378.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9019..9444
                     /locus_tag="ACG8OO_000442"
     CDS             9019..9444
                     /locus_tag="ACG8OO_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9044379.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9437..9886
                     /gene="nusB"
                     /locus_tag="ACG8OO_000443"
     CDS             9437..9886
                     /gene="nusB"
                     /locus_tag="ACG8OO_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9044380.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            9983..10837
                     /locus_tag="ACG8OO_000444"
     CDS             9983..10837
                     /locus_tag="ACG8OO_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFP9044381.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10838..12175
                     /gene="xseA"
                     /locus_tag="ACG8OO_000445"
     CDS             10838..12175
                     /gene="xseA"
                     /locus_tag="ACG8OO_000445"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9044382.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12180..12404
                     /locus_tag="ACG8OO_000446"
     CDS             12180..12404
                     /locus_tag="ACG8OO_000446"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9044383.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12406..13296
                     /locus_tag="ACG8OO_000447"
     CDS             12406..13296
                     /locus_tag="ACG8OO_000447"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9044384.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13305..14123
                     /locus_tag="ACG8OO_000448"
     CDS             13305..14123
                     /locus_tag="ACG8OO_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFP9044385.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14230..14679
                     /locus_tag="ACG8OO_000449"
     CDS             14230..14679
                     /locus_tag="ACG8OO_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9044386.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14697..16379
                     /gene="recN"
                     /locus_tag="ACG8OO_000450"
     CDS             14697..16379
                     /gene="recN"
                     /locus_tag="ACG8OO_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9044387.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16434..16550)
                     /locus_tag="ACG8OO_000451"
     CDS             complement(16434..16550)
                     /locus_tag="ACG8OO_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFP9044388.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16623..17579)
                     /locus_tag="ACG8OO_000452"
     CDS             complement(16623..17579)
                     /locus_tag="ACG8OO_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9044389.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17750..18118
                     /locus_tag="ACG8OO_000453"
     CDS             17750..18118
                     /locus_tag="ACG8OO_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFP9044390.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18288..18719
                     /gene="mraZ"
                     /locus_tag="ACG8OO_000454"
     CDS             18288..18719
                     /gene="mraZ"
                     /locus_tag="ACG8OO_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9044391.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18740..19699
                     /gene="rsmH"
                     /locus_tag="ACG8OO_000455"
     CDS             18740..19699
                     /gene="rsmH"
                     /locus_tag="ACG8OO_000455"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9044392.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19704..20105
                     /gene="ftsL"
                     /locus_tag="ACG8OO_000456"
     CDS             19704..20105
                     /gene="ftsL"
                     /locus_tag="ACG8OO_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9044393.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20106..22298
                     /locus_tag="ACG8OO_000457"
     CDS             20106..22298
                     /locus_tag="ACG8OO_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9044394.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22324..23286
                     /gene="mraY"
                     /locus_tag="ACG8OO_000458"
     CDS             22324..23286
                     /gene="mraY"
                     /locus_tag="ACG8OO_000458"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9044395.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23300..24661
                     /gene="murD"
                     /locus_tag="ACG8OO_000459"
     CDS             23300..24661
                     /gene="murD"
                     /locus_tag="ACG8OO_000459"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9044396.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24687..25775
                     /gene="murG"
                     /locus_tag="ACG8OO_000460"
     CDS             24687..25775
                     /gene="murG"
                     /locus_tag="ACG8OO_000460"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9044397.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25790..27010
                     /locus_tag="ACG8OO_000461"
     CDS             25790..27010
                     /locus_tag="ACG8OO_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFP9044398.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27166..28491
                     /gene="ftsA"
                     /locus_tag="ACG8OO_000462"
     CDS             27166..28491
                     /gene="ftsA"
                     /locus_tag="ACG8OO_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9044399.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28514..29755
                     /gene="ftsZ"
                     /locus_tag="ACG8OO_000463"
     CDS             28514..29755
                     /gene="ftsZ"
                     /locus_tag="ACG8OO_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9044400.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29772..30449
                     /locus_tag="ACG8OO_000464"
     CDS             29772..30449
                     /locus_tag="ACG8OO_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFP9044401.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30463..31062
                     /locus_tag="ACG8OO_000465"
     CDS             30463..31062
                     /locus_tag="ACG8OO_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFP9044402.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31143..31364
                     /locus_tag="ACG8OO_000466"
     CDS             31143..31364
                     /locus_tag="ACG8OO_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFP9044403.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31413..32195
                     /locus_tag="ACG8OO_000467"
     CDS             31413..32195
                     /locus_tag="ACG8OO_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFP9044404.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32216..32923
                     /locus_tag="ACG8OO_000468"
     CDS             32216..32923
                     /locus_tag="ACG8OO_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFP9044405.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33185..35983
                     /gene="ileS"
                     /locus_tag="ACG8OO_000469"
     CDS             33185..35983
                     /gene="ileS"
                     /locus_tag="ACG8OO_000469"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9044406.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36032..37558)
                     /gene="zwf"
                     /locus_tag="ACG8OO_000470"
     CDS             complement(36032..37558)
                     /gene="zwf"
                     /locus_tag="ACG8OO_000470"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9044407.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37573..38220)
                     /locus_tag="ACG8OO_000471"
     CDS             complement(37573..38220)
                     /locus_tag="ACG8OO_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP9044408.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38404..38733)
                     /locus_tag="ACG8OO_000472"
     CDS             complement(38404..38733)
                     /locus_tag="ACG8OO_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFP9044409.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38910..39638
                     /gene="sfsA"
                     /locus_tag="ACG8OO_000473"
     CDS             38910..39638
                     /gene="sfsA"
                     /locus_tag="ACG8OO_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9044410.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39654..40667
                     /locus_tag="ACG8OO_000474"
     CDS             39654..40667
                     /locus_tag="ACG8OO_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9044411.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40667..41944
                     /locus_tag="ACG8OO_000475"
     CDS             40667..41944
                     /locus_tag="ACG8OO_000475"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9044412.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42007..44709
                     /locus_tag="ACG8OO_000476"
     CDS             42007..44709
                     /locus_tag="ACG8OO_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9044413.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44861..45178
                     /locus_tag="ACG8OO_000477"
     CDS             44861..45178
                     /locus_tag="ACG8OO_000477"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9044414.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45208..45528
                     /locus_tag="ACG8OO_000478"
     CDS             45208..45528
                     /locus_tag="ACG8OO_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9044415.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45636..47096
                     /locus_tag="ACG8OO_000479"
     CDS             45636..47096
                     /locus_tag="ACG8OO_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9044416.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47164..47385
                     /locus_tag="ACG8OO_000480"
     CDS             47164..47385
                     /locus_tag="ACG8OO_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044417.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47416..47598
                     /locus_tag="ACG8OO_000481"
     CDS             47416..47598
                     /locus_tag="ACG8OO_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFP9044418.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47598..48011
                     /locus_tag="ACG8OO_000482"
     CDS             47598..48011
                     /locus_tag="ACG8OO_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFP9044419.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48134..49315
                     /locus_tag="ACG8OO_000483"
     CDS             48134..49315
                     /locus_tag="ACG8OO_000483"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9044420.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(49386..49550)
                     /locus_tag="ACG8OO_000484"
     CDS             complement(49386..49550)
                     /locus_tag="ACG8OO_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044421.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            49704..49777
                     /locus_tag="ACG8OO_000485"
     tRNA            49704..49777
                     /locus_tag="ACG8OO_000485"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49738..49740,aa:Arg,seq:cct)
     gene            complement(49823..49936)
                     /locus_tag="ACG8OO_000486"
                     /pseudo
     CDS             complement(49823..49936)
                     /locus_tag="ACG8OO_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(50319..51551)
                     /locus_tag="ACG8OO_000487"
     CDS             complement(50319..51551)
                     /locus_tag="ACG8OO_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9044422.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT        17129 a         8813 c        11116 g        14682 t
ORIGIN      
        1 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
       61 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
      121 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
      181 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
      241 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
      301 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
      361 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
      421 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
      481 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
      541 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
      601 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
      661 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
      721 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
      781 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
      841 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
      901 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
      961 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     1021 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     1081 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     1141 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     1201 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     1261 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     1321 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     1381 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     1441 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     1501 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     1561 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     1621 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     1681 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     1741 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     1801 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     1861 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     1921 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     1981 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     2041 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     2101 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     2161 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     2221 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     2281 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     2341 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     2401 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     2461 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     2521 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     2581 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     2641 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     2701 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     2761 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     2821 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     2881 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     2941 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     3001 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     3061 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     3121 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     3181 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     3241 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     3301 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     3361 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     3421 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     3481 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     3541 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     3601 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     3661 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     3721 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     3781 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     3841 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     3901 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
     3961 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
     4021 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
     4081 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
     4141 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
     4201 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
     4261 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
     4321 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
     4381 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
     4441 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
     4501 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
     4561 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
     4621 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
     4681 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
     4741 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
     4801 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
     4861 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
     4921 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
     4981 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
     5041 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
     5101 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
     5161 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
     5221 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
     5281 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
     5341 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
     5401 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
     5461 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
     5521 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
     5581 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
     5641 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
     5701 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
     5761 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
     5821 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
     5881 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
     5941 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
     6001 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
     6061 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
     6121 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
     6181 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
     6241 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
     6301 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
     6361 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
     6421 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
     6481 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
     6541 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
     6601 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
     6661 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
     6721 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
     6781 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
     6841 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
     6901 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
     6961 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
     7021 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
     7081 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
     7141 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
     7201 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
     7261 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
     7321 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
     7381 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
     7441 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
     7501 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
     7561 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
     7621 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
     7681 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
     7741 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
     7801 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
     7861 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
     7921 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
     7981 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
     8041 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
     8101 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
     8161 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
     8221 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
     8281 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
     8341 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
     8401 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
     8461 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
     8521 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
     8581 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
     8641 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
     8701 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
     8761 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
     8821 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
     8881 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
     8941 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
     9001 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
     9061 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
     9121 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
     9181 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
     9241 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
     9301 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
     9361 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
     9421 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
     9481 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
     9541 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
     9601 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
     9661 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
     9721 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
     9781 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
     9841 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
     9901 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
     9961 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    10021 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    10081 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    10141 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    10201 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    10261 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    10321 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    10381 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    10441 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    10501 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    10561 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    10621 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    10681 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    10741 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    10801 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    10861 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    10921 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    10981 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    11041 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    11101 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    11161 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    11221 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    11281 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    11341 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    11401 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    11461 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    11521 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    11581 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    11641 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    11701 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    11761 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    11821 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    11881 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    11941 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    12001 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    12061 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    12121 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    12181 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    12241 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    12301 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    12361 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    12421 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    12481 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    12541 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    12601 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    12661 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    12721 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    12781 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    12841 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    12901 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    12961 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    13021 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    13081 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    13141 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    13201 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    13261 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    13321 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    13381 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    13441 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    13501 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    13561 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    13621 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    13681 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    13741 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    13801 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    13861 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    13921 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    13981 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    14041 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    14101 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    14161 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    14221 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    14281 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    14341 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    14401 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    14461 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    14521 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    14581 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    14641 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    14701 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    14761 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    14821 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    14881 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    14941 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    15001 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    15061 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    15121 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    15181 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    15241 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    15301 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    15361 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    15421 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    15481 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    15541 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    15601 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    15661 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    15721 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    15781 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    15841 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    15901 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    15961 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    16021 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    16081 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    16141 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    16201 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    16261 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    16321 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    16381 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    16441 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    16501 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    16561 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    16621 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    16681 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    16741 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    16801 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    16861 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    16921 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    16981 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    17041 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    17101 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    17161 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    17221 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    17281 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    17341 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    17401 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    17461 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    17521 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    17581 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    17641 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    17701 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    17761 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    17821 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    17881 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    17941 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    18001 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    18061 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    18121 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    18181 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    18241 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    18301 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    18361 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    18421 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    18481 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    18541 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    18601 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    18661 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    18721 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    18781 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    18841 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    18901 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    18961 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    19021 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    19081 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    19141 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    19201 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    19261 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    19321 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    19381 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    19441 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    19501 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    19561 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    19621 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    19681 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    19741 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    19801 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    19861 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    19921 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    19981 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    20041 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    20101 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    20161 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    20221 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    20281 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    20341 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    20401 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    20461 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    20521 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    20581 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    20641 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    20701 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    20761 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    20821 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    20881 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    20941 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    21001 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    21061 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    21121 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    21181 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    21241 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    21301 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    21361 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    21421 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    21481 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    21541 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    21601 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    21661 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    21721 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    21781 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    21841 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    21901 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    21961 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    22021 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    22081 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    22141 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    22201 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    22261 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    22321 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    22381 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    22441 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    22501 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    22561 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    22621 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    22681 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    22741 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    22801 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    22861 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    22921 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    22981 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    23041 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    23101 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    23161 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    23221 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    23281 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    23341 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    23401 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    23461 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    23521 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    23581 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    23641 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    23701 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    23761 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    23821 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    23881 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    23941 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    24001 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    24061 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    24121 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    24181 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    24241 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    24301 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    24361 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    24421 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    24481 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    24541 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    24601 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    24661 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    24721 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    24781 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    24841 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    24901 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    24961 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    25021 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    25081 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    25141 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    25201 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    25261 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    25321 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    25381 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    25441 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    25501 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    25561 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    25621 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    25681 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    25741 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    25801 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    25861 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    25921 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    25981 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    26041 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    26101 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    26161 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    26221 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    26281 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    26341 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    26401 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    26461 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    26521 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    26581 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    26641 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    26701 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    26761 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    26821 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    26881 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    26941 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    27001 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    27061 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    27121 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    27181 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    27241 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    27301 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    27361 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    27421 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    27481 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    27541 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    27601 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    27661 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    27721 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    27781 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    27841 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    27901 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    27961 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    28021 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    28081 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    28141 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    28201 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    28261 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    28321 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    28381 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    28441 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    28501 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    28561 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    28621 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    28681 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    28741 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    28801 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    28861 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    28921 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    28981 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    29041 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    29101 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    29161 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    29221 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    29281 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    29341 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    29401 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    29461 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    29521 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    29581 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    29641 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    29701 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    29761 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    29821 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    29881 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    29941 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    30001 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    30061 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    30121 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    30181 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    30241 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    30301 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    30361 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    30421 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    30481 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    30541 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    30601 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    30661 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    30721 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    30781 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    30841 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    30901 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    30961 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    31021 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    31081 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    31141 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    31201 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    31261 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    31321 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    31381 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    31441 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    31501 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    31561 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    31621 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    31681 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    31741 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    31801 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    31861 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    31921 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    31981 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    32041 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    32101 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    32161 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    32221 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    32281 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    32341 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    32401 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    32461 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    32521 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    32581 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    32641 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    32701 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    32761 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    32821 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    32881 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    32941 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    33001 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    33061 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    33121 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    33181 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    33241 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    33301 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    33361 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    33421 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    33481 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    33541 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    33601 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    33661 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    33721 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    33781 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    33841 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    33901 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    33961 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    34021 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    34081 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    34141 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    34201 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    34261 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    34321 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    34381 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    34441 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    34501 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    34561 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    34621 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    34681 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    34741 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    34801 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    34861 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    34921 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    34981 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    35041 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    35101 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    35161 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    35221 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    35281 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    35341 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    35401 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    35461 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    35521 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    35581 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    35641 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    35701 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    35761 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    35821 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    35881 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    35941 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    36001 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    36061 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    36121 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    36181 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    36241 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    36301 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    36361 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    36421 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    36481 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    36541 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    36601 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    36661 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    36721 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    36781 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    36841 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    36901 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    36961 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    37021 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    37081 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    37141 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    37201 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    37261 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    37321 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    37381 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    37441 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    37501 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    37561 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    37621 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    37681 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    37741 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    37801 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    37861 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    37921 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    37981 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    38041 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    38101 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    38161 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    38221 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    38281 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    38341 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    38401 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    38461 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    38521 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    38581 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    38641 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    38701 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    38761 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    38821 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    38881 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    38941 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    39001 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    39061 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    39121 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    39181 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    39241 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    39301 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    39361 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    39421 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    39481 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    39541 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    39601 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    39661 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    39721 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    39781 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    39841 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    39901 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    39961 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    40021 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    40081 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    40141 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    40201 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    40261 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    40321 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    40381 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    40441 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    40501 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    40561 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    40621 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    40681 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    40741 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    40801 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    40861 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    40921 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    40981 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    41041 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    41101 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    41161 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    41221 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    41281 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    41341 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    41401 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    41461 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    41521 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    41581 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    41641 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    41701 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    41761 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    41821 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    41881 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    41941 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    42001 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    42061 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    42121 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    42181 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    42241 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    42301 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    42361 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    42421 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    42481 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    42541 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    42601 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    42661 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    42721 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    42781 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    42841 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    42901 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    42961 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    43021 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    43081 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    43141 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    43201 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    43261 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    43321 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    43381 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    43441 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    43501 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    43561 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    43621 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    43681 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    43741 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    43801 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    43861 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    43921 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    43981 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    44041 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    44101 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    44161 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    44221 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    44281 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    44341 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    44401 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    44461 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    44521 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    44581 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    44641 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    44701 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    44761 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    44821 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    44881 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    44941 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    45001 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    45061 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    45121 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    45181 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    45241 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    45301 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    45361 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    45421 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    45481 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    45541 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    45601 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    45661 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    45721 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    45781 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    45841 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    45901 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    45961 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    46021 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    46081 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    46141 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    46201 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    46261 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    46321 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    46381 aactgatcat tgatttggtg caaaaatata ttgcagagcc attcctagga ttatctcaag
    46441 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    46501 atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    46561 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    46621 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    46681 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    46741 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    46801 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    46861 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    46921 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    46981 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    47041 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    47101 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    47161 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    47221 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    47281 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    47341 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    47401 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    47461 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    47521 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    47581 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    47641 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    47701 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    47761 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    47821 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    47881 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    47941 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    48001 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    48061 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    48121 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    48181 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    48241 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    48301 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    48361 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    48421 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    48481 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    48541 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    48601 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    48661 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    48721 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    48781 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    48841 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    48901 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    48961 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    49021 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    49081 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    49141 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    49201 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    49261 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    49321 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    49381 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    49441 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    49501 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    49561 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    49621 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    49681 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    49741 aagcgatagg tcgctggttc aattccagtc aggaacgtac aaataacccc tgtatccttt
    49801 gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg ctatgtccta
    49861 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgcgtat
    49921 gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt actaaagaaa
    49981 aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga attaaacgat
    50041 ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa ctctagcggt
    50101 ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt tttattattt
    50161 ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc atataaaaaa
    50221 acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc ctttattcta
    50281 tgctttatat tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg
    50341 aaaatcggta ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct
    50401 accatgaaat ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg
    50461 gacatttctg taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc
    50521 gatgcgtttt catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa
    50581 gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt
    50641 tttgctaaga catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa
    50701 gtaaatttca tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg
    50761 taaccatcta ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc
    50821 atgaattttt ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc
    50881 aatccgataa tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga
    50941 ttcaattctt ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg
    51001 tcttcttcat agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt
    51061 tcttctggca gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca
    51121 gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc
    51181 gctgattgat aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca
    51241 gaaacaacgc tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta
    51301 gattgtggta cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc
    51361 tggtcgtcag tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg
    51421 atcaatcgag caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt
    51481 tctgtggcta cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa
    51541 tcagataaca taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac
    51601 catttaatta tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag
    51661 gttaagtttc atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac
    51721 taattttgca tatcaaaaaa
//
LOCUS       DAZKBH010000008        50714 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000008 DAZKBH010000000
VERSION     DAZKBH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.008"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..1466)
                     /locus_tag="ACG8OO_000488"
     CDS             complement(102..1466)
                     /locus_tag="ACG8OO_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9044423.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1463..3022)
                     /locus_tag="ACG8OO_000489"
     CDS             complement(1463..3022)
                     /locus_tag="ACG8OO_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9044424.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3078..5558)
                     /locus_tag="ACG8OO_000490"
     CDS             complement(3078..5558)
                     /locus_tag="ACG8OO_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044425.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5542..5877)
                     /locus_tag="ACG8OO_000491"
     CDS             complement(5542..5877)
                     /locus_tag="ACG8OO_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044426.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5891..6925)
                     /locus_tag="ACG8OO_000492"
     CDS             complement(5891..6925)
                     /locus_tag="ACG8OO_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9044427.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(7015..7695)
                     /locus_tag="ACG8OO_000493"
     CDS             complement(7015..7695)
                     /locus_tag="ACG8OO_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9044428.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7979..9082)
                     /locus_tag="ACG8OO_000494"
     CDS             complement(7979..9082)
                     /locus_tag="ACG8OO_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFP9044429.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9711..10181)
                     /locus_tag="ACG8OO_000495"
     CDS             complement(9711..10181)
                     /locus_tag="ACG8OO_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFP9044430.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            10511..12151
                     /locus_tag="ACG8OO_000496"
     CDS             10511..12151
                     /locus_tag="ACG8OO_000496"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFP9044431.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            12305..13627
                     /locus_tag="ACG8OO_000497"
     CDS             12305..13627
                     /locus_tag="ACG8OO_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFP9044432.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13695..14129)
                     /locus_tag="ACG8OO_000498"
     CDS             complement(13695..14129)
                     /locus_tag="ACG8OO_000498"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9044433.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(14194..15031)
                     /locus_tag="ACG8OO_000499"
                     /pseudo
     CDS             complement(14194..15031)
                     /locus_tag="ACG8OO_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(15773..16399)
                     /locus_tag="ACG8OO_000500"
     CDS             complement(15773..16399)
                     /locus_tag="ACG8OO_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044434.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(16694..17029)
                     /locus_tag="ACG8OO_000501"
     CDS             complement(16694..17029)
                     /locus_tag="ACG8OO_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9044435.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(17016..17300)
                     /locus_tag="ACG8OO_000502"
     CDS             complement(17016..17300)
                     /locus_tag="ACG8OO_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9044436.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(17356..18879)
                     /locus_tag="ACG8OO_000503"
     CDS             complement(17356..18879)
                     /locus_tag="ACG8OO_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9044437.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(18872..20047)
                     /locus_tag="ACG8OO_000504"
     CDS             complement(18872..20047)
                     /locus_tag="ACG8OO_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9044438.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(20051..20236)
                     /locus_tag="ACG8OO_000505"
     CDS             complement(20051..20236)
                     /locus_tag="ACG8OO_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044439.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(20202..21896)
                     /locus_tag="ACG8OO_000506"
     CDS             complement(20202..21896)
                     /locus_tag="ACG8OO_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9044440.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(21893..22366)
                     /locus_tag="ACG8OO_000507"
     CDS             complement(21893..22366)
                     /locus_tag="ACG8OO_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFP9044441.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(22435..22590)
                     /locus_tag="ACG8OO_000508"
     CDS             complement(22435..22590)
                     /locus_tag="ACG8OO_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFP9044442.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(22724..23104)
                     /locus_tag="ACG8OO_000509"
     CDS             complement(22724..23104)
                     /locus_tag="ACG8OO_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9044443.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(23108..23323)
                     /locus_tag="ACG8OO_000510"
     CDS             complement(23108..23323)
                     /locus_tag="ACG8OO_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044444.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(23326..23733)
                     /locus_tag="ACG8OO_000511"
     CDS             complement(23326..23733)
                     /locus_tag="ACG8OO_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFP9044445.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(23999..25474)
                     /locus_tag="ACG8OO_000512"
     CDS             complement(23999..25474)
                     /locus_tag="ACG8OO_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFP9044446.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(25464..26312)
                     /locus_tag="ACG8OO_000513"
     CDS             complement(25464..26312)
                     /locus_tag="ACG8OO_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFP9044447.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(26349..26711)
                     /locus_tag="ACG8OO_000514"
     CDS             complement(26349..26711)
                     /locus_tag="ACG8OO_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044448.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(26712..26870)
                     /locus_tag="ACG8OO_000515"
     CDS             complement(26712..26870)
                     /locus_tag="ACG8OO_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044449.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(27011..27635)
                     /locus_tag="ACG8OO_000516"
                     /pseudo
     CDS             complement(27011..27635)
                     /locus_tag="ACG8OO_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(27632..27943)
                     /locus_tag="ACG8OO_000517"
     CDS             complement(27632..27943)
                     /locus_tag="ACG8OO_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044450.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(27987..28280)
                     /locus_tag="ACG8OO_000518"
     CDS             complement(27987..28280)
                     /locus_tag="ACG8OO_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044451.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            28474..29121
                     /locus_tag="ACG8OO_000519"
     CDS             28474..29121
                     /locus_tag="ACG8OO_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9044452.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            29182..30327
                     /locus_tag="ACG8OO_000520"
     CDS             29182..30327
                     /locus_tag="ACG8OO_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9044453.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(30408..30713)
                     /locus_tag="ACG8OO_000521"
     CDS             complement(30408..30713)
                     /locus_tag="ACG8OO_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFP9044454.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(30738..31169)
                     /gene="ruvX"
                     /locus_tag="ACG8OO_000522"
     CDS             complement(30738..31169)
                     /gene="ruvX"
                     /locus_tag="ACG8OO_000522"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9044455.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(31166..31432)
                     /locus_tag="ACG8OO_000523"
     CDS             complement(31166..31432)
                     /locus_tag="ACG8OO_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFP9044456.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(31687..32412)
                     /locus_tag="ACG8OO_000524"
     CDS             complement(31687..32412)
                     /locus_tag="ACG8OO_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFP9044457.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(32773..32843)
                     /locus_tag="ACG8OO_000525"
     tRNA            complement(32773..32843)
                     /locus_tag="ACG8OO_000525"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32809..32811),aa:Gly,seq:ccc)
     gene            32960..33712
                     /locus_tag="ACG8OO_000526"
     CDS             32960..33712
                     /locus_tag="ACG8OO_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9044458.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            33728..34207
                     /locus_tag="ACG8OO_000527"
     CDS             33728..34207
                     /locus_tag="ACG8OO_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9044459.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(34458..36038)
                     /locus_tag="ACG8OO_000528"
     CDS             complement(34458..36038)
                     /locus_tag="ACG8OO_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFP9044460.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(36031..36774)
                     /locus_tag="ACG8OO_000529"
     CDS             complement(36031..36774)
                     /locus_tag="ACG8OO_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044461.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(36950..37726)
                     /locus_tag="ACG8OO_000530"
     CDS             complement(36950..37726)
                     /locus_tag="ACG8OO_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9044462.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(37974..38810)
                     /locus_tag="ACG8OO_000531"
     CDS             complement(37974..38810)
                     /locus_tag="ACG8OO_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFP9044463.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(38821..39750)
                     /locus_tag="ACG8OO_000532"
     CDS             complement(38821..39750)
                     /locus_tag="ACG8OO_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9044464.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            39942..40295
                     /locus_tag="ACG8OO_000533"
     CDS             39942..40295
                     /locus_tag="ACG8OO_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9044465.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(40369..41025)
                     /locus_tag="ACG8OO_000534"
     CDS             complement(40369..41025)
                     /locus_tag="ACG8OO_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9044466.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            41251..43185
                     /locus_tag="ACG8OO_000535"
     CDS             41251..43185
                     /locus_tag="ACG8OO_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9044467.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(43271..44494)
                     /locus_tag="ACG8OO_000536"
     CDS             complement(43271..44494)
                     /locus_tag="ACG8OO_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9044468.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(44488..46359)
                     /locus_tag="ACG8OO_000537"
     CDS             complement(44488..46359)
                     /locus_tag="ACG8OO_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFP9044469.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(46380..47498)
                     /locus_tag="ACG8OO_000538"
     CDS             complement(46380..47498)
                     /locus_tag="ACG8OO_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9044470.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(47581..48414)
                     /locus_tag="ACG8OO_000539"
     CDS             complement(47581..48414)
                     /locus_tag="ACG8OO_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9044471.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(48407..49324)
                     /locus_tag="ACG8OO_000540"
     CDS             complement(48407..49324)
                     /locus_tag="ACG8OO_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9044472.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(49551..50675)
                     /locus_tag="ACG8OO_000541"
     CDS             complement(49551..50675)
                     /locus_tag="ACG8OO_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9044473.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        15094 a        10236 c         8549 g        16835 t
ORIGIN      
        1 tatgctcaaa aaattcttcc gctatttcat tatatgaatt ttttattctc gattgattaa
       61 tatttattat atccccacac aataaaagac cgtgatattt ctcacagtct tttatctact
      121 ctattgctat ttgctctctt agctagtact ttaaaagcta actcattgag gtagtgaaaa
      181 cgagaggtgt gttcagacag aaatccaaaa tcctcaactg ctagctgtgg aatcgtagtt
      241 ttgtgtttca cgatatccgc aagcaggttc aaatctgata ataagaattc ttggcttatc
      301 agctgtccgc caattatttc taatgtatcc ggatggtaga tcagctttgc atttactgtt
      361 ttgttttctt catcgtattg tggaaataga tcaaattctc ctgaacacga accggtattt
      421 tgttccaaga atggcgcttc atcttctgtc agccctacag ttcctaaaaa gtagtctata
      481 atgactgctg ctgttatctt ttgtctgagg tcgtaagcgg ttggctgacc actcaaagtt
      541 agcgctactg ctcttgccat atgtacagct cgagtaacta aaggtaaaaa gagatgccgt
      601 ttatttttgt caaaactgac tgggacgata tctccaatcg catagatgtc cggatcactt
      661 gtctgcagat attcattgac tatcacagtc tcatcctcat acaaagccac tttttctttt
      721 aacaatgtcg tattaggacg agtttgggca gaaaaaatga ccatatctgc ctttattgct
      781 tgagatagaa gttgtacgga tactactttc tcttctgatt ttttccaatc gatcaagaat
      841 tcatttggat aaaaattgat ttgcttagtt tgcagctgat tttgaaaact gttgatcata
      901 tccttatcga aatatcttga taaaacattc ggcatataat caactagatg aataatgtat
      961 cctttatttc tcaaagcatc tgctaattcc aatccaatat aaccagcacc gaagatcact
     1021 acttcttttg cttcttttaa agcggcaagt acttgcttag attgcactag atttttatag
     1081 gtgtagacat tttcagcttc aatttgttta tacaaggtga caggagagga acctgttgct
     1141 acgattaatt tatcataggt tatggtctcg ttttttcctt ctttggatgc gaggatgagc
     1201 tgatgtttgt ctggatcaag ttcagttact ctccattcgg tgatcagttt tgccccactt
     1261 tcttgaagag tccgcatgtt atgggcaact tcagctaaac tacgtatctt tccttttaaa
     1321 taaaggttga ttccatttga aacatagcct agtccatctt tatgatcttc atcgatcagt
     1381 aaaacttctg ctttcggttg aagttttctt agatttaggg cagctgtgat gcctgcatgg
     1441 gaagcaccaa tgatcacgat tttcatgttt tattcatcct taaattcaat ttttctaaat
     1501 aacattcttt taaaaattgg ttcaaatagg ggaaatcata attatattcg ttgctagtga
     1561 atacaaaaac tttagcggac ttctgtgacg ggtactcttt caattggcta gtcaaaatga
     1621 tatcatactg cttttctttc tcgtattcga ctaaatcata tttgatcgta ttcagcaatt
     1681 gttcctggat gatttctttc acgatatccg ccattagcct ttcataagga agatcataag
     1741 caatttgcag acgtaattct aactgcttga tcagcgtccg caaaatagat gcatagcgag
     1801 caaataattc aaagcgggca ttttcctcgt ctacttcagt aaaatcaaca atagcaaatg
     1861 tttgtctgac caatctttca cataaaacca ggaattgttt atcttctact tcagttaacc
     1921 gctgttttaa tccttcttgg ccaaaaatat tcatccatcc tctaaaatag gcaaaatgag
     1981 cgtgattttg catcactgtc aacgcaatct tttgcgaaat ctcttctgga aaaacataaa
     2041 aagagaaata ttcttctatc gattgtttaa acgttcgatt caattgagct actacgtcgt
     2101 ctttgtattt ttcgcttatc aaaaactcct ggacatgcga gttatgtgta tctaatgtaa
     2161 agttactgat catgaaaaga taaaaaacca acgtttcatt ttgattccaa ggaaaggcat
     2221 attgaaagaa gtaacgggtc aaaaatgtat ggaggtccaa taaaaattgt gtcgtttgaa
     2281 acaatccaac aggaagattg ctataatctg gtttggattt tttcgtacag cgcattcgct
     2341 tcttcataat ggaaagccat atttcaaatt tcaatcgtcc ttcttctgta atagtaaaac
     2401 taaactcttt ttgcagcaga tctaaaaaag tgtttcctga taaatcactg tattcttttt
     2461 tgttttcaag ataaggacgt ccaaaccaaa ataattggaa ataaaaataa caaatttgac
     2521 tttctggacc ggtcagccgc ccgcttttta ttttcagatg aaactcattt aaagaatcat
     2581 ttaattcctt cactctgcga gacaaagtag ccgaactgat attaagttcc atgacaagtt
     2641 tctgccgatc aaatgagcca ttttccaaca gataaagaag tatctgatat tttgtagaat
     2701 agcgcaagta gcaatgataa taaacatcga ctgctgctat atttccatgt ctgattaata
     2761 aaatattccc ttcttggata acaatttcca attgtttgat attttccaag ttagcttgta
     2821 aataaaacag atcttctttc aaggtagccc gatcaactga taagcgggta agtacgttat
     2881 taacggaaat ctctcctttt tcctctaata aaagttggaa aagtttgatt tgccgttgtt
     2941 catttttttc aagcaaactg aaaagttcca agtccactcc tccaagcatt tctgatttta
     3001 acagcagaag tttccttctc atccttgaaa aagaggatct gtgacagtta tcacagatcc
     3061 atacaaattc taatcactta gctggttggc ccagctgtca gttcccatgt gaccatgcct
     3121 tgatacgttg cgccaggttg agcattcgta ctgctattta cctctaagag taaaccatcg
     3181 gtatcgggaa agtcaacgag aacctctcct ttagctgttc catcactctc ataaatcaac
     3241 gtgttttgac cttgctgaat caattgtttt gatccagaat gattgaagta taattttgtt
     3301 ccgcttaatt cttgaccttg atcattctta aataaagaag tcaatgtccc tgtcagttgc
     3361 caaggagacg tgcttggaag ccgagtatcc cgcacatcaa aagaaaaatc gccttttgct
     3421 tcataataac ttgtcttcaa cggtttgggg agatcgttac caaatgtaaa atcatctggt
     3481 acattaacta atgttaattc ttgtttgggg acaagaattg ttttagcaga tgtttggaca
     3541 actgggttag gaagcttagc accatctgct aatgtaatat tgctggaaag tacagctgcc
     3601 tttgtcagta tgctgacgga ttgtacagaa gtatctacag tatttaattg atagttgtaa
     3661 gtaagatgaa tggtatctcc tattttgctc aatagatctg ctgggacaga aactccttgt
     3721 tgaatgccgg tatctgcgat agttgtacca tttaatttta aggaaccatt gatataagta
     3781 gtattttcag gaagtgagat cgtgattttt ttgtcggtta tcgcatcccc agattttgtt
     3841 aacgtgaatg tcacattttc ttgaatcgta tctcctgata gagcagacag gggatctcct
     3901 gtctcaggtt gtctgctaag gttttttagc tgactatctg cactcacaga tggaaaatta
     3961 gccactgtta tttgaggaag agtggtgatt ccagcttgtt cagactgcca tttgtacgca
     4021 aaagacggat acatgttagt aagtacatcg tccaccaagc gataatcaaa ataatattgc
     4081 cctgaagcca atgcaggtag tgtagaagta gctgtattta cacccccgtt atttgattgg
     4141 atcgtttgga aaagttgatc tgtccatcct gtcgctaatt tttgggttcc tttcgaataa
     4201 agcctgtaat ggatcgaacc cttttttagt tcagttgctg accaattggc cgtgacttgg
     4261 tacgatgcag aagaaaggag ctttccccac tgactatcgg ctgttaagtt tgttggtaaa
     4321 accgttccgt ctagcttgta ctgctccaat acctttaaag caacaggaga aagcaatccg
     4381 ccttgcgcat ctttcccact gtacacgata ttggctgtct gattcaatcc acttgtgctt
     4441 gctggagtat gagtcagtat tgctccttgt gtaacgtttt ttcctgtaaa atagttgact
     4501 acgcgatcaa agcgactaaa tggcaactga tcaaaacgtc cttcagtcag ccccatcgtc
     4561 aatgtccgac tattggtaga gcctggaagg ttctggttat ttccatccac gtattctgtg
     4621 gtatcagcaa atgtattata ttttcctgtt gaatcacttg ttgagatgta actattccac
     4681 gtatttatct ctgcacttgc agaaaatgca tatgctcctg gattcaagtt tccgccagtt
     4741 aatttaggat aaaatctacc tgcagctgga gctaaaatcg aattgaacat acgatcttgg
     4801 ataatattat tcgaatcata aaaatcaaca gaaaaaacgg cctttttaga catagaaggt
     4861 tgtaatttac ccttggtcaa aattttgttt ggactcgtat catatttatc tccgttttct
     4921 gtcatcttcg cgacattagc tgtaacatca aattgaagcg ggatatcgtc cacgttgaat
     4981 tcgcctcgcg taataatagc aataatatct gccaatagag tatttgtatc ccacgacccc
     5041 atcccaaaac gcaaggtatg gcttcctgct gataataaac taccgctagt aaccttgatg
     5101 ggggcaccgg caatatacaa gtaggcgttg ttccagtcaa ttgcttttaa tacatcactg
     5161 cttgatacat gttccggcaa attgatgatc gccacaaatg caaatccttg tgcagtgata
     5221 ttcaccaaat caagtacatt tgttagtttc acatggcttt cccaatgaaa acggaccgtg
     5281 ccacctttcc aaggatcatt tgaaccatcg ttcaaataag tagcagtcaa tttaggcaat
     5341 tggttagccc cagttagttg gacaagcttc ccgccattga ctgcactacg tccaccattt
     5401 ggaggtacga tattttcata cgtgtatgga tcataagaaa tggcaggagg cgttgctcca
     5461 acagcttctg ccgaaggtga aaagataaat atacttaaaa caaaaagcga agtaaaaaag
     5521 aaggagcgaa aaaatgaaaa tttattttgt agcaacatgt tcatcctcct tttcctttct
     5581 tctccataac aacaaccacg caagaagcca taattctata ccaatgatca aataaagaaa
     5641 acggatcttt tcttcattcg tctccgggaa tacgccatgt tgattagatt ctttataaat
     5701 cggtagctgt gtctgctgtt ttgatgcgcc attgactgaa ccacttggat gcggaactgg
     5761 acttagatca taaaatgtaa tcgtacctga acttttggta tctgcttgga ctggctcttg
     5821 ccagctgaag aacagcaaac ttgcaataat ccctcccaat aagatcgatg ttttcaacga
     5881 aatcacctca tcatgttgtt tctccttttt tctttttctt gtggacccac aaaaacagca
     5941 aaatcaacca taacactaga actgcagcaa ccaatcctgc ataaagccat gggaaagatt
     6001 gttcttgtac tgcaaaaacg gaattttcat ttactttttg tgctttttct tttttgatcg
     6061 taaagttttg tgtaaatcgc cattcattac tgtcgcctga ttttgctatg atttctgcgc
     6121 ggtaatcgcc ggcttttgct ttatcaccag ctaaatcaag attcaagtga aaattcgaat
     6181 ttggtgcaac taaaaattca ttagacgtat tttcaatata aagctgattt tcataaaaga
     6241 tcttgctcgt aaatatcaaa ttgctgataa tcctaggctg aggattttgg aaacgaagct
     6301 gaacagttgg ctttgcgttg acctcgtcca aatctcctga taaaagtttc agatccggtt
     6361 ctatcgtctc ttgactttct tgtaaaacga ctgcgagatt ataagcaata cgatttttga
     6421 tttgtgctcc ttctgtagca ttttctgttt cagcttcttc tttaggtgaa acatgaatac
     6481 ccgctaatac acgtccctta aagctttttg ctgggatttt cacttctata ggtatagttg
     6541 tttctgcatg tgctgataca ttgatttcct ttttcgcaat gagcaaaacg tcacggacat
     6601 caaacggcaa tgtctcatct aacgttgggt ttttttgact ataatcgatc gttccaccat
     6661 cactcgttac cgccggattg acagagacct caaatgttcg ttcttcatct gaagaattgc
     6721 tgataattac ttctagtgtc tgattttgtt ccggtgccac taataaatca aaataaccga
     6781 ttgctttatc tatttggttc tctggaaata ccggtttgat tccaaagtct cctgctgaat
     6841 cctttgcttg gctgattgac ggaaaaatga aaaatagact taaaatagaa aaaaacgaaa
     6901 caaacagtcg tttactttga ttcattgtcc accacatcct ttcaacaaca actgcggtaa
     6961 aaaatccttc acctatagta ttttcaccgc agtttgttga atttatttca tactctatgc
     7021 tactgggcca gccacgaggt tccaagtcaa atcagccgtg aatgttccag ctgctgcttt
     7081 cggcactttc agtgtgatat ttgctggatc ataaacagaa agccatgttc ccagaccttg
     7141 atcttttgct gctgtaaata ttggtgtcgc tgcacttgct ttactcaacc cagttacagt
     7201 ttgactagtt ggtccagtgg attcgttatc tgacgtactt ttaacagtgg atgttccaaa
     7261 atctaattca gcgccttgca aaacttgtga accgtttaca aaatctgaaa ccgaaactgt
     7321 cacgttccac ccttgtgctt ggccatctgc acctacacct cgacgatctg atacttggag
     7381 ataaggtgta tcgttttttg aagcgtctac ttgatacgtt tttgttccgc tttcaatctc
     7441 gtgtgtacca aatggcaatt caggaactac atccaatgtt aaagaacctg tctggccagt
     7501 tgctggattt tccggatctg ctggctctac tggttttgaa ggatcagtag ggttaagagg
     7561 ctcaacggta tttgttggtt cagtaaaggt aatatctcct tttgaagtcg cttgaccctc
     7621 agcaaaagct ggtgttgcca agccgcctag aagaagtgtt gaaagtaata atgtcccaat
     7681 catgttcttt ttcataataa atcactcctt tttcttagtg acctaagtat agtagagaca
     7741 gtatgcaaaa aatcctcatg aattcatccg cttacttgaa attatgaccc tttttgtata
     7801 aaagagaaaa aaacaataaa atcgtttaca tgaatattct tcctttttta ttaagtatga
     7861 aaaaatattt caattttatt ttttcgtttc aaattattct attagtgaca tccttgaact
     7921 ataaaaatag gagtacggca tcatctgccg cactcctatt tttttcgtca tttatttctt
     7981 aaataaaacg attctcacgc aattttattt ttttgatatc ttctactgtt tgtgttccag
     8041 ctaattgcat gaccatttcc aattctgttt taaagaaatc aaacacttga tggactcctg
     8101 tacttcctcc taaagacaat ccataaataa cgggtctgcc aatagcaaca agatcggcac
     8161 ctgatgcgat tgctttgaaa acatgttgtc cacgtcgtac accgctgtca aacacgattg
     8221 gtacacgttt atctactgct tctgccacat attgcaacga atcaaacgca gcaggccccc
     8281 catctaattg acggccacca tggttagata cccaaattcc accagcacca gattctaatg
     8341 atcgatagac atcttcttct gattgaacac ctttcacgta gactggcaaa tcagaatgag
     8401 cagcgataaa ttctacatct tttggactaa gtttttgttt ggaagattta tatacagcat
     8461 ccatcgtttg gcctactcct gattgataag cctgaacgat cggcattggc agcgggaagg
     8521 taaacccgtt tctgcggtct gtttcacggt ttccgccaac tgttgcatcc gctgtcaata
     8581 cgattgcttt tgctccatta cgcttggcca cttcaagaat atccaaattg attccattat
     8641 ctttgctcat ataaaattga aaccattgcg gtgctttctc cccgcctgct tctcttatct
     8701 cttctaaagt acaagaagcg taagaacttg ctgtatagat cgtcccaaaa tctgctacac
     8761 cttttgcaga agctttctcc gccttcacat gagctaatcc atgagcagca actggtgcca
     8821 tgatgattgg tgcagtcagc atttcttcgt caaaatgtgt agttgtatcg ggtaattcta
     8881 catctcttaa tacatgagga ataatcaaac gatgattgaa tgcccgttca ttttcttgat
     8941 aagtaaataa gtcgccagca ccactgctga tgtaaccata accaccagct ggaatcactt
     9001 gtgaagccgc gatttctaaa tcttccatat tgataaaatc tactattcct tcatttgtac
     9061 ctgcttgata tgttttttcc atctcaatgc cttctctcta taaaatttga atgatgaaca
     9121 atatgatagc gtttacactt tttatattac tcttcgacat tgatacaggc aaataatctg
     9181 tttacatttc agccattcca gatgtcacta ctctggaaga aatgattgta ataaaaatag
     9241 ataaaaaacc gatgggactt tgacaaaagt agtgtcaaag tccctttttt tgaataaacg
     9301 gtgttcaaaa gccattttct cggtattaag tcaaatttat gcaaaaatgt ggaaagccat
     9361 tttcacaaaa tttttcttac tactcgaaac taagcgactt tttcacaacc tctcgataaa
     9421 acggtgttca atcagctgac cctcggtatt agctcaaaaa atggcaaaat atgaggaact
     9481 attttgccat ttgtctctct aatactcagg tcagaacgtt gacttcacag tctcttcctt
     9541 aaacggtgtt caaaaacttg catctgcggt aataagccca accaaacgaa aaataggagg
     9601 agctattttt gtttggctgt tcttattact tgatgcaggg caggtttttc acgacctctg
     9661 attcacggtg tttctttttc atcaacattt ttacgaaata ctgtggaaga ctattgaaat
     9721 aagtgtggat tgtttctttg tgtttcaatc gtttcttgcc attcttcatt taattttgga
     9781 ttaccgtttt cttccttgaa ctcataaggt cctaattcaa aaatcccaga cagtatttga
     9841 ttcacgattt catatagttc agtgtgatca gacacatgat tcttgaagat catctcatta
     9901 tcaccacgtt tgaacctcac attcgggctc tcatgatgaa cctctatgca tttttctcct
     9961 ttttttaact gacggacttc tgaaaacatc tcataaatat cttctccagt aaaaccttca
    10021 gcaagcgcca gactgtccac aagaccgcct actgtcagtt cagagattat tgcttgattg
    10081 gaatctaatt ttaatgtata gcttgtcgct tctactacgc cacatgcatt gcaaccgcct
    10141 tctgatagtt cgctttttct aatcaattga acttctttca ttttctcttc ctcctcttaa
    10201 ttttctactt atgtattttt atcacctgac ataggtgact cctatcaacg ctatcatgtg
    10261 cttttttcct cagaaatcct atttatccat tccatctatc ccttattctt ctacttttct
    10321 ttctctagca tataagagat acgcatagta ttcaagcaag gattgtcgat ataaaaagaa
    10381 caaatactgc tattttgatt tcttttggct atattacata taaataaaat ttatcatacc
    10441 tgctcttttt gactatatca tcatctgact tctatacttc gtttgtatta aaacttagga
    10501 ggaatcattc atgcttacag ggttaaactt attaaataat ccttttctaa ataaaggaac
    10561 agcttttaca aaagaagaac gggcaaaata tggaatcact ggtatgcttc caagtactgt
    10621 ccaaacactg gaacaacaat ctgttcaagc ttatggtcaa tacttaagca aacaaacaga
    10681 tttggaaaaa cgtatcttct taatgaatct attcaatacg aatcggacat tgttttataa
    10741 attgatgggc gaacatttag ttgaattcat gcctgtcgtt tatgatcctg tcgttgcgga
    10801 ttcaatcgaa caatacaacg aaattttctt gaaaccgcaa gatgctgcat ttctttcaat
    10861 cgacgaacca gaaaacatca aagcaagttt gaagaacgcg gcagatggac gagacattcg
    10921 tttgattgtt gtgactgatg cagaaggaat cttaggtatg ggcgactggg gtgtgaacgg
    10981 tgttgacatc gctatcggca aattgatggt ctatactgct gctgctggta ttaatccagc
    11041 acaagttttg cctgtatcga tcgacgctgg tactaataat gaagaattat tgaataatcc
    11101 cttatactta ggcaatcgtc atgctcgtgt agaaggcgaa acttactatg aattcatcga
    11161 tcaattcgtt caatcagcta ccgaattatt cccagaattg ttattgcatt gggaagattt
    11221 tggacgagga aatgctgcag cgatcttaga aaaatacgaa gatcaaatca ctactttcaa
    11281 cgacgatatc caaggtacag gaattgttgt cctagcaggt gtattaggcg gattgaatat
    11341 atccggtgaa caattgaaag atcaaataat tttgacattt ggtgctggta ctgctggtgt
    11401 gggtatcgca aacatccttt tagatgagat gatccgtcaa ggtgtacctg aagaagaagc
    11461 acgccaacat ttctatcaag tagataaaca aggtttattg tttgaagata cagaaggatt
    11521 gactcctggt caaatcccat tcgctcgcaa acgctcagat ttcactaatg ctgatgaatt
    11581 gacaaactta gaagcagttg taaaagcgat ccatccgaca atcatgattg gtacttctac
    11641 ccaacctgga gcattcactg aagaaatcgt gaaagaaatg gctgctcata caccacgtcc
    11701 gatcatcatg cctttgtcta atccaacaaa attggcagaa gcaaaagcaa aagatttaat
    11761 agaatggacg gacggaaaag cattagttgg aacaggtatc cctgctgctg atgttgaata
    11821 taatggtgta acttaccaaa tcggtcaagc aaataatgct ttgatgtacc caggtttagg
    11881 acttggtttg attgcttcaa cagctacacg agtaaatgct gaaatcattt ctcaagctag
    11941 tcgagcatta ggcggaatcg ttgatgtaac aaaaccagga gcagctatct tgcctccagt
    12001 agcaaaaatc actgaatttt ctcaaacgat tgctgaaact gtcgcaaaat cagttgtcgc
    12061 acaaaacttg aatcgtgaag aaatcacgga tatcaaagaa gcagttgaat ctgctaaatg
    12121 ggttcctgaa tataaatctt tagaagatta agatcgttat agtaaaagaa catacagaaa
    12181 ctttgtatga cgtctaaaaa gaaaaaagcg actgcggcaa aacagcggcc agccaactcc
    12241 tgctcttttg tcgcttttct ttggcttttt tcatatgata cagaataaga aaggatctgt
    12301 aaaaatggca caaaaaacaa caactgcaac tgcagttgag gaaaagaaaa aagaaggttt
    12361 ttggtccaca aaaatcaccg gcgtaagttt gccactttat ttgatcatgg tccttgtcct
    12421 gatcattgca atggctttag ggaaattacc gacgaatatg attggaccaa tcgcagtttt
    12481 ggttattttc ggtaatttat ttcattttat cgggacaaaa atccctatcg tcaaaagtta
    12541 tttgggtggt ggttccgttt ttgcgatctt cgcttctgct gcgattgcta catttgggat
    12601 cttacctgaa tatgtagtga aatccacaga aaactttgtc agcaatatgg gcttttggga
    12661 cttttatatt gctgccttga ttactggtag catccttggt atgaaccgta atctattgat
    12721 gaaagcttct gttcgtttta ttcccgttgc acttatttca atggtcgtct ccttttttgc
    12781 cgtaggactc gtgggcatgc ttatcggaaa tggttttgca aattccgttt tatacatctc
    12841 attacctgcg atggctggtg gtgttggagc cggagccgtt cctttatcaa cgatttatgc
    12901 aaatgtttta ggtacggatg cttcttctat catttcccgc ctgattcctg cgacagcaat
    12961 gacaaatgtc ttagctatca tcggtgcagc attagttgct cgtgctggac aatcaatgcc
    13021 aaaattcaac ggcaatggta aattaatgga aaaaggcgat cttggcgatg gaaaacctcg
    13081 aactgtgaaa ccttccattc cacagttagg tgttggtttg atggtatcat tgactttctt
    13141 catcttagga caaattggta actattttgt tcctaaagtc catgcttacg catttatgat
    13201 tattatcgtt gtcatctgta aaatcgccaa cctattacca gagtattatg aagatgcagc
    13261 tatcatgttc aataacttga ttgtcaaaaa cttgacagca gctgttcttg ccggtatcgg
    13321 tattgcgttg ctgaatctga atgtattagc ttctgctttg acatggcaat ttgttgtctt
    13381 atgtctgaca agtgttatcg cgatttcagt tgtttcagga tttgtcggtc gtttgtttgg
    13441 tttatatcca attgaatcaa ctattactgc tggcttatgc aacaactcga tgggcggaac
    13501 aggaaatgta gccgtcttat ctgcagctaa tcggatggaa cttattgctt ttgctcaaat
    13561 gggcaatcga cttggtggtg ccatcgtttt gatcatctct ggtttcttga tgcaattact
    13621 ttcttagtca aaaaaacaaa aatacctgat catcaagtat cggcttggtg atcgggtatt
    13681 tttttgataa agatttacag gattttcaaa aatctttttt gcattttatc ggaatgataa
    13741 ccgatgtgtt cataaaattg gtgtgcttct actctgtatt ctgcagaatt aagccttatt
    13801 gtcgttattc ctcgtttttt cgcttcttct tctacgccag tcatcaaagc ctttccgatg
    13861 cctctttttt cagcatattt agacacagca agtgccataa tattgaacat tggatcgcta
    13921 aaaatcgatt cataggactc cgcttgtaca tagccagctg cttttttaga agtaggatct
    13981 tcataaacca agaaaaaatt tttctctggc tccttcagca gtttcttcat ttgtctcacc
    14041 gtaacggtta atggtatatc ataccctaat tgatccgcat tgatttcacg tagtgcttct
    14101 gcatcttcta aacgcatttc ccgtaacatc caaatcacca gccaatttct gattttttaa
    14161 aatgaatttt tttatcatag tatactcttt ttttcattta gaacaatatg tttcagtgat
    14221 tttgacttgc gttagtctag attctcctaa tgtttgtaag atgacaaagg cattgtaaca
    14281 attgaccaaa agaaccacga ggacaattac ttgaaaaata aaggtcactt tttttggaga
    14341 caaaaccctg ctaaatcttg ctccccacaa acctccaata acagcagcgg ggataacata
    14401 aaacaacata gataaatcaa agcgcataaa gctacttgtt aaagcaatcg ttaccaattt
    14461 tgccagttga gaaaagaaaa tcgtagatag agaatatagt gttgcttctt ttattggcaa
    14521 tgcaaacatc agcatcaaca aagaaacatt gactggtcca ccaccaatac ctaaaaaact
    14581 agctaaaaag cccagaaccc cccccacata taaggtacca agtccatgac gtcaaatgaa
    14641 attttggctg atgatgcttg gtgtagaaga acgcaaaaac caaggtcaat accgtcaaaa
    14701 aaatttggat cagctgcaca tgcttctcgt tttcaaataa ctgtaaaaaa acttcgaata
    14761 caatgttgcc gactactcct ccaacaacag ctccactaga tacccaaccg acaatctgcc
    14821 aattcagtga tttcccgctt gatacttgtc gcacagtcga aacaatggac atcgtgaata
    14881 ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg ggcgccgata agatcaagaa
    14941 tcggtttgat caacacgcct ccacccatcc cggaaatagc gccaactgtg ttcgccagaa
    15001 cgatcaccac aaaataaatc agaccaatca tcttgtattt cctttctctt gtttatatct
    15061 tttagcgatt gttactcttt tcttttatta tacagtaaaa gaaacaaacg aggcttcgaa
    15121 agcttttcta ctttttaagg aaatttagtg ctaaacgaaa agaggttgtg aaaaagctgt
    15181 cctgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcataattt tcgtttggtt
    15241 gaacttatta ccgcagatgc aagcttttga acaccgttta agaaagaggt cgtgagcctg
    15301 ccgttcagct ccgagtcaca aggaacaatt tcaaaaaaca gcttctcatg tttgttgaat
    15361 attgtgactt gtgaccgagg agttggcagg agaacaccgt ttaaggaaga gactgtgaag
    15421 tcaacgtttt gacctgatta ttagagaaac aaatggcaaa atagttcttc atatttcgcc
    15481 gtttttcgag cgaatgccga ggggcagctg attgaacatc gtttatttgg aaaaagggat
    15541 tgtgacaaga cgtttgtcac aatccctttt tttatatctt attaattcat tcttttgttc
    15601 tattcttctt cttccgccat gaatgagaaa cccatatcaa agaactataa aggttatcaa
    15661 aacagcgttt atgactaaat gaattagagt tatctatcac tatttttcaa gcgatttaaa
    15721 ccaaatggca tacaatatgg catacaaaaa cttcaataag ccgtgggggt ttttaaattt
    15781 cttcttgttc tttaaaatca atgagtggag ctacataatt tgaatccact ttttcaaacg
    15841 tcactttttg catcgacgaa tgttttcttt cccaagataa cgtaaataga tttgactctg
    15901 gacttataaa gtctacttca ccttctagta ttactttctt atgcaaatat tcctctaata
    15961 aatatagact tgctaaagca tacaacacat tttccaaatt tgcttcctta taatttgaag
    16021 aacgaccatg ttttactctg ttatatattt tccaccaatc tggtgtatct tcaggccacc
    16081 catctccaaa aggttttagt gaagtcaatg ttaaagaatt cataacccta acaccattat
    16141 ccaaacacat taattcgcta gatttaataa cagctttaaa atcagttatg cttttattgc
    16201 tactgctatc aaatccgcaa agttctttga aaataatctc acactccgaa ccaatcgcta
    16261 gtagtagttt ttgatattca atagagaatg ttttgaaatt attctcatcg agcatcacaa
    16321 aatctgtggt attaataaac tcattttcta aatgtagata atacttccaa taaaaatttg
    16381 taaactctgc tttgtccatt aaatcacctc ttttgaatta taacaaaaaa aacacgtcca
    16441 caacagacgt gttctttgat ttctacacaa tcagcttgcc aaaaccatcc aatcagtcct
    16501 agcatgcctg tattaaaatt gaacgttcat catctatcat ctttcttagt ccagcatgtt
    16561 tgcaaccaca cctacaattt tcagtccaac aatagatttc gactagctaa gtcttgcaaa
    16621 attcaggcag ctaacccttc atttactacc ttaattatac taaataccca caacgtttac
    16681 aagtgtaatc attctaagaa atctgtttag ctatgatcgt tgtataatct ttaaacgccg
    16741 ttccttcatt gattgaaata attttataac tcttgttttt ccaagctact ttcatatcgt
    16801 ttgttagttc ataatcttga tcgtatcgaa taataaacgt caacgttcct tcaagaatgg
    16861 tacctacact agcaagcacg tcttttagca tttgtgaacg cacattagcc caacattcaa
    16921 atacagtttc ttcactatcc accatctgcc catcttcatc ttttacccgt tttgtctttt
    16981 taaacttgac tctctcagtc aattcattta cattcttaat gattggcata ataagcacct
    17041 ctcatttgtt gtactaatgc ttgtaccgtt actggtatcc gttcgctgct ggcttcttga
    17101 cggttcaaat accaatgttg agtaagtaat gacaccgcca aatcaaattg ctgataatta
    17161 tccatatctt tatccgttgc gctactatca atagcacttt ttatatattc gcctgctgta
    17221 tcaatcaatt gttgaataaa gtcatcatca atagtgtgat cgatccgcat actttttttt
    17281 atgctatcca aaattaccat tttatctctc ctaatgaaaa aaaggcagtg ggataaaccg
    17341 ccgcccaata tttttctata cttttggtgt tacaactggc gtaaactcaa tatacactga
    17401 agcattttga tcaattttct tataatcgtt gcgaacgatc actgcaagtc cttgtgagta
    17461 gtaatcgaat ttttcccatt gagtcgttac ttgatttcta cgagcaacaa agatagattg
    17521 tgcaatatcc cccataatca tagggaatgt tccagcttta ggattggcaa acaatgtatc
    17581 tgcaatcaat actactggca taccgaataa tgatttacca ctaggtgctg tcacatcggg
    17641 ttgtaaaatg taacgtccat ctgaatcttt caatgtatct aggtggtaat atccgctttg
    17701 gtttagaatt accattttat ttaatgctgg gtctaatgcc acattgtata gttgtttcaa
    17761 atcgtccaac gtagcagccg ttttcttagt gaatgttttt aataaatcca tgatatgctt
    17821 attgtcagtg ttttctacca atttcgctaa ctgatctttg acctcttgta caatattcac
    17881 tgctgaatct tccacaacct catttgataa ggcaatctta ccagcacgag tttctacttt
    17941 ataatcaact gaagtaaaca tttcagcgtc aatatcacca atttcagcaa gttcagcttt
    18001 tgttgctaac actgcttgtt ggttagttgc tactggatat ttaccttgtc catttgatac
    18061 tgtttttact gttgcatatt gagctaagtt ataatttgaa cgtttcaaat caaagacttc
    18121 accgattact tcttcgggaa caactgccgc tgcatttaca gtagtgactc catcacgcac
    18181 ttcgccttgt gaacggatat attcttcata cccacgcaat tctgtttttt cgttatcgat
    18241 taaagttttt tccatgttat tactctcctt ttgtgcttta gaagtggatt ctaagctatt
    18301 gtttgtttgt tttgcttggt tgttactcat aaacgattcg taagaacgct tatttacttg
    18361 gacatttgat gaatcataag cgggaacggt cacgacacta atttcattaa gtgctttgat
    18421 tttatctatt gatcgttcaa ttgtgccatc ttcttttttg tcgaatgaat ctaatccaag
    18481 cacgaaccca aaggacatag aatcaacaac cccttttgag atattctcat acacatcatt
    18541 tgaataggtc gtgtcggtta gctctgcttc aaaatgtaat cctgtatcat cgacattcaa
    18601 tttcaacgta cctgctttga cactagctag tggctttgaa taatcgtgtc catataataa
    18661 gaacacattt gataaatcca cctcttttaa tgcttcgggt gtaatcactt ctacaaagcc
    18721 gcctaaatct tttgacggtt gcccgaattt caacgcatag cctgaaatag ttttgccgtc
    18781 tgttggttgt ttttcatttt ctttctctac tttgggagaa tcggctgtta gctcagcttc
    18841 ttctgtcagc cgtttttcct tttcttcttc attattcaac tggtgtaact ccttcctgtt
    18901 cttttaattg atatttctcc aacgtatcta aatacgtaaa gttcaaactt gctagtaaac
    18961 gatcgccacc atccatgggg ggtagcccca ttttcgaccg cccctcatta atcgtgagaa
    19021 gtgacccttt gacctgatcc aatacatttt ttacttttgt ttcaggatca gtctctagta
    19081 actgatcgga attaaagcgg atattggttt caagtttcgt atctaattca ctcacaaaca
    19141 cattaaagta atgaatcagc gtactttgga tatactggag attactttga acggtgctag
    19201 aatgttcatt ttcaacgcct aaacgctcaa tcggtacacc aaacgccttg gcaatttgtt
    19261 ttgtgttcca atcattggaa ttgataagct tcaaaacatc ggtgtttatt tctaatgttt
    19321 tgtagtccat tgtttcatca agaatgatcg ttctaagagc attatctcca ctacttgaac
    19381 cgttggcttc ttcaaacttt tcacgaattg cgttctttgc tgatccgtct aaatctgact
    19441 tatgaacctt caaaatacct gacccactga caccacgagt aaagaaattg tgcaatgtgc
    19501 ggttgccagc ttgttgaatt ttcagttcat cacgtaacgc ataaagtggt ggtaatcctg
    19561 ttaaaccatc ttgggtgaaa tatttgaagt gcaaaatatc gctagacttc acacgtcttt
    19621 ttttatcacc aatctgataa gacaacgtac cattatctaa ttgagtaacc gttacacttg
    19681 agttaggtaa taagtgaatt tcttctacct tttcaccgtt gcgtttaatc tctgcaaagc
    19741 tattaccgtt tagcaacata ttgaccgcca aagcaaactt taaatgccaa ccatccattt
    19801 ctgaattagg tttctcgttt agtaacttca ccagttcatt atcagcttgt ggcatattgt
    19861 ttttgaccaa ttgaatcgga cttgaagcaa tatcactagc aataattcga acggctgcaa
    19921 acacatcact atttcttaat gcacgaacgc ttgtaaagct ggtggtgtaa tcatccgatt
    19981 ggattgatac cacatgttgt aggaaaggat ctcccgttgt attttgtctc gctttaaaaa
    20041 atgccatctt ttaacctccc ttctgttctg tatttagcat gaccgctacc ccaataagca
    20101 ctatgcccag cgctaacaag ccatagtaga catttgttag taaggtgata gcaaccaaca
    20161 cacacaccaa tccaagaagt aaaagaacgg tatgaatgta attagaaaga aaattcttca
    20221 cttgtataaa attcattgtc tgctttcgct ccttccgttg tggtgaagta atccattgca
    20281 aacacatagg cattgattaa tgcagctatt ggatcaattt tgttactgtt ctttgattta
    20341 ttaatctgaa tgccgttgtt atccgttttt aaaatagcgt tgttcactgc atgggtgaga
    20401 atcgtatttt tattgtgaat aacgttgcct tcataaactt gttctctaaa agttcgggtc
    20461 ggaacgttta gagtaatcgt tccttgtctt acttctaaca ttgggtagtt ggctttttca
    20521 gctttactga taagcgaatt agcgttatac gggtcataac aaatccccat acattccaaa
    20581 tcattttctt gaatcaaatc ttgaataaac tgaaatactt ggtcatagtc cacgattcca
    20641 ctttctaatt gagtaatgga acactcacca gctttttcta gttctcgata aggcaaaccg
    20701 tcgcgctttt ctttgtcttg aagcccatat ttggtggcta caaagctatg tgaatcacaa
    20761 taaagttcgc cgttatctag cggtacgatc catgaaacgc tagttaaatc atctgtttta
    20821 gataaatcca ctccgatata aacgggcttg cctgtaatat cttgtggttc tacttcggtt
    20881 gcctgccagt cttcagcaat catatagctg tcctcactcg cttgtctcca taaattgaag
    20941 tttttcacca atacagaatt aaggttattt tgtttcaaag caaggttcac atcatcttga
    21001 atggttggaa tcattacttt tttgatttct tcactttcaa aaatcggatt ggctttgatc
    21061 catttctctt gatcgtgaat ttcttcggga tcgtccaatt cccatatcgc tataaagtac
    21121 ctgtcggctt gttcttcgcc ttttagcaca cgatctaaca ataagtattc ctcgtacata
    21181 ggaacgttta aatcaagccc agcggtacta ataacacaca ataagccgtt cttttgttgg
    21241 gtcattcctg acttaatgac gttgtacgtt ttacgagttt ttgcttcgtg ccactcgtca
    21301 agaattgcgg ttgttggtgc aaacccatct agcgtgctgg tttcactagc cagtgccata
    21361 gcgaacgaat tagagggcaa gtgctgaatc ttagagttca taattttcag ttgtggtctt
    21421 aaaaacttac tagacttgac cacgtttcta agtgaattag acagcatatc atagcccaat
    21481 ttagcttgtt tcaaagcgtt acttacaaac aatacttgcc ttgcttctgc tggttcttgt
    21541 tccataatca acgcattggc agccatgcca cttgcgatat acgttttccc attcttacgg
    21601 ctcatactgg taaacgctcg attgaatcgc ctgtaattcc cttctttagt acgccagcca
    21661 taaagactgc ctagaatgaa tttttgaaat cctaacatct tgattgcttt accgtccgta
    21721 gatggtagca actcaatgaa tttaatcgct ttggtggctt gttcttcatc gaacacataa
    21781 gggaagctat ctgacttaga acgctccaaa tcgcttatat gacgctcaca cgcttggata
    21841 attttcttgg gtgcaatcag cttacctgat aggactttct caatgtaatc attcatgaat
    21901 tcatcaactc tttgaacggg tcttcgggtt catcattttt aaccttgttc aaagctagtt
    21961 ttgatcgtga ttctaaggtc atacctagtg aggtggcttg ttttttcaaa tcactcatag
    22021 cttgtgattg aacccgaacc gctggattgg ctttctttac tccgttttcg catgtgataa
    22081 acgtacctga ttttcttatc tcggcttcgg ctgttttcat tcgagcaaag gctaaacaat
    22141 aggctaccaa tgaaccataa tctgcttcac tcaatggata atcttttttt aatgttggta
    22201 cgatacgatt ccattcactt ctagcgctcg ctgccatcca gtcagggggc gtgatattta
    22261 ttaatggttg ctgcttgaac aattcttctc tagcgtccac acggattgct ttttcttcac
    22321 ttgatatatt gccttttgta tcgtgtaata ttttagggtg tcccatgttg tcctcctttc
    22381 agacaaaaga aaaaggaacg taagcaatta agctcacatt ccttcgtttt tcgatcagaa
    22441 tttttcttta tctaatttat ctatataaat gatttttccg ttctgcattt gaatggtcac
    22501 ttgaccaaaa tgaggtaact caatggtgcg ctgtttccct gttttatcag taataacaat
    22561 tgtatttttc aataaatctc ctactttcat tatatacttt ttcacttgtg attacaagtg
    22621 taatcgttat ttgtaattac ccttatgcct tttcacaacg gttatttgta ccaaaatgag
    22681 taggcatcga ttgtcagtaa gtttcacact atgccccctt aactcaaagg gtactctttt
    22741 ctcgtctttg tctttcattg gctgtcttgg aattatggtg tgcatggcat aaagaacgaa
    22801 aattagtttc atctaaccgt ctattccaat catcttttag ttcaattttg tgatctacaa
    22861 catcagcttt ccttatcaag ccttcttcta ggcaatcctc acatactgga tgatttaacc
    22921 gatacaagta tgaagccttt cgccatgttc tactttgata gaattgaaaa tatttacctt
    22981 cgctttgctt tctttcttca cgcttaattg ccgtttgctt tgctttatgc ttcccacaat
    23041 acttctgcct ataatcaatc aatgttttac atccagcatg attacattgt tttttaactg
    23101 ccatagatta ccaaccaaag cgatctcgtt cgtcagaatc atcttgttcc cattttgatt
    23161 tgtattcaat ataaggcgaa actatcttca agcacgttcc gtctttaaaa ttaactttag
    23221 tgattttatc ttcctcaaaa ctaataaagt ctacatcttc ttgagtgttt gaatactcaa
    23281 tgtgtttctc accatcgaag tatctaatac tttccatatg cataattatt ctacctccaa
    23341 taaattagtt atcaattcat catctgttag tagcaaagcc aagaacttat catggatgtt
    23401 taaaaacaaa ttatcctcat tcgatgtaag ctctcgttgt ctcgctcgat catataggtt
    23461 aagcagacaa ttaaattcat ccattagctc acctgtatca tcatagttgc cactatctac
    23521 caatgatccc actactagcg attgacacca cgctactgac tcctgcagcc aatcaggttc
    23581 ttcttccatt atctttaata cttctggatc aaatgtggaa taatcttctg ttaccaatcc
    23641 cacactaaat gccagcgtgt tcattagaaa ccgatccatt tcatctgtat attgaaagtt
    23701 cggtgctttg tttgttgaaa taatattgag catttacttt cctccttgat tcgtaacacg
    23761 tggtaacacc tacgtaacag gtacccgtta ccactcaatc ccaacgggga caagggttct
    23821 agcgttttgg taacaggtaa cgggtaaaat ccagtaacat tattccccag catattagct
    23881 aactatttca aaagttttaa ggaaataagg tgttaccccg ttaccaaatt gctgtatccc
    23941 ttggggcagt aagggtaaac tagtaacggg ctaggtgtta ctgacccgtt actacccgtt
    24001 actttttgta ctttccagct cgatttttat tttcataggc atttgactta ttgtaaaatc
    24061 ctcgtcgttt atttcttttt ggcagttgct tccgttccca accaggaata ctgctaataa
    24121 tcaaggcaat ctttttagtt tctgcatctg atcgaccatt caataaatcc tttgcttgtt
    24181 tatcaaaaac agcttccaaa atatcagcgg tgagtacgct atcaactaaa taaaactctt
    24241 gttgcatagt agctccatta tagaaatgct gtaaattttt ctcattacat ttactgtcag
    24301 gatagttctg ataataagaa cgtctcgccc acgctggagc ttcttcccat ttatacggga
    24361 tttccatttc caaatattta gtgattgctt ctttaattgg attctccatc ttggcatcct
    24421 cttgatagtc ttttgcaatt tctaagactt ctttatcttc atcgggacta aaatagattc
    24481 gttgtccttt atcaaaaagc acctttgctt cagctaacac ttgaagaaaa tattcatcct
    24541 ttgttttaaa cggattcatc tttggcttat tcttacaagg caaaggataa aaacggcggt
    24601 ttcctgtatc atctttcaaa tactgcccat cattcgacgt tccaataaat acacattgcc
    24661 ttgcccaagc tatgacattc cgaccataag gaggtctaat tttatcgatc ttggcactga
    24721 tgaagttttt tatctgttca attttagttt tgttcattga agataattct ccaagttcaa
    24781 taattacatt gccttgaagc tgcatataat catctttctt ttcacctagc gaatctaatg
    24841 aatcggtgaa aaaatcatcc gtgtaaagta atgctggaac ggtactctta ccaatccctt
    24901 gcggtccatc caaaactgga acgatctcaa actttattcc tggtttatag atacgtgcga
    24961 ctgatccgac tatccaacgt tttgttactt ctcgtgtgta aaggttgtcg tctaccccta
    25021 aaaggtcaat aaacagtgtt tccagtctag gctgttgatc ccatttaact ttttcgatac
    25081 gttcttttac tgggttgaaa acattctttc tagccacatt tactactgct ttgtgtattt
    25141 tgttgtcatc aaaaactaca ttgtagtttt cttcaatata tgccgttaag gcatcctcaa
    25201 aactgtcttc ccattctcca gcggtacgat tgatccaagg agaatctttt aataggtgaa
    25261 tgtatccgct aaattcgtta taagcaattg ttccagctaa attgctatca ttggttaaaa
    25321 tcaattctgc attcacaaca ctgttacttt taatctgatc gtctttattg catcttaatc
    25381 gtgacatcca ggttttcaat tcaatgatat tgtcttcacg ctcgctggct tcgggaaagt
    25441 ctaaaatact atctaattta tgcttatgag ccatgagctt tccccctcaa atctttttta
    25501 atcatcgatc gaataatttg attcacttct ttatctttta gcggtggttc attgtacgca
    25561 tttgctgtat aaatcaattc tgctaaagtt tgattatcta caccgtgcca tagtaaccaa
    25621 ccgcataact tagttaaatg atcgtttcta ctaccttcgc cagcgccttg aacaatcata
    25681 tctaattttt ttgctgtccc tgtttttgct acgggtcggt attgagtctt tatttcttgc
    25741 ggttttttta gcagtaggtt cactagccaa tcgggagctg gtgcaatatt atttagagat
    25801 acgttttcaa taggttcata tgctctccca acacttcttg aaataatcat agttggcgct
    25861 attggaactc ctttagtgat aatatcaatc ccgcttaaac ttttaccttt atcatcacgc
    25921 ccgaagatat cagcaaatgc acctgtttct tgttggataa ttacaccatc aggaatttta
    25981 aagaagaaat ggattccttt acctggtgtg atttccgtat aagtagaagg aaatggatca
    26041 tacattgaag caagtttttt ataattctca acgccgtttc gtgtttcaga atgttgatct
    26101 aggtcaacta ccaccatttg atgatcttca agcattaacc caatatttct agttgcatct
    26161 tccgtccacc attcattaat ttgattagaa tcgttagttg catcattcca accactagag
    26221 ccttttattg gaattttagt tccaggagat aagggataaa ttgaccatcc cgacttttgt
    26281 aattccgttg ctgcttgata attatttgcc aataaacctc tcctcctttt tcaaaatact
    26341 ggtataaatc attcagacac ctccatgagc ttcaactgct caagttcgta tttcaaccca
    26401 tcccgaacaa attcagcacc ttttaattga ttctgatact ttacccatgt attaaatgtt
    26461 ttcacccttg cttcttgcat atcagtaatg tttaaccatg cggttaaaat ctgatttttt
    26521 agctcgttga tttctcgaca aattattttg tgttctgcat gttttttgag tacatacaaa
    26581 actgttagtt tttcattgaa tgaatttcca aagtaggttt tcccgatatt attttctagt
    26641 tcagcgatcc cttgattaat ttcctctaat tcaaggctat cgagttggtt taatagtttt
    26701 gttttgtaca tctacttatg ccctccagtt cttaattgat agtcgtactc gtcataagca
    26761 gtgaagagat ataacgctaa tcctgcaaag atagctattt ttgcaattaa tggtaataag
    26821 ccgaaaattc cgccgattaa aatgcttcct ataattaagt ttcgtttcat aatggttccc
    26881 tccgattttt taatatggta taatcggaac aaagaatatc gggttttatc ccaattaagt
    26941 tgcctttatc ctgtccgcca agactgttat ggataaggct ttttctttgt tctcgatcat
    27001 gttaatgctc ctaagttgtc tgcaattcag cagaacgatt ataaattttc tcactcactg
    27061 aaaactgcaa cccatatttt tctttaacag tcattaattc tactgtctca tctaaaatag
    27121 gttcacgatc aaccaacatt tcaggagtca tatctttctt ttttaccatt ttggaatgac
    27181 cataaatagt tgatacagct ttattggcta tcacattcgc tttaatgtag tctactggtt
    27241 ttggttcagt aatagcgtgg cttaatttag tcattgcttc tttttggtgt tctttgtcta
    27301 gcatgcggaa tacttgaaag ccttcaagtc ctgtggactg gcgtagttct ttgatgatgt
    27361 cgaaaatcca gtcttgaaat gcctcggctt cttttttacg gcttttgaat gccatgcggt
    27421 aaatattttt tttcgttgat gatgtttaca tcttgtgttc tgcccaatga atcgatgacc
    27481 ttactagtag taaccccatc tttaggcaaa gagtgaatgg ctctagtaac ctgtttaaga
    27541 cctaacgctt ccgcaacatc tttcgccact gcccaccatt catcattaat atcgacaaat
    27601 cttataatgt gtccgttcca aatttcagtt ttcattcaac gtcctccagt tcaagcccta
    27661 gcaagctcgc taacaactca acttgctgat acaaaatttc ttgatactct tcaatagaaa
    27721 gtcgttcttt aaactctgct tgtactaaat cgttcaagcc acgcaatact ccgcttaccg
    27781 ttggttcttg tggtagcaat ttttgatagt ccatttgttc gtacaaacat tcaaacgttt
    27841 cttctagcgc tttgccttcc aagtttgcta attctgccgc tgtgtcttct gtaagaattg
    27901 ttgataatac tttttcgatt gctttatttt gtgtcgattc cattattatt tcctcctatt
    27961 aatccctttg cccccacggc ttgcgtttat ttttggtatt cttccataaa gcgatcacat
    28021 tcatcttgtg aaattcttac gactccatca actataatca actttaaacc gttaggaaca
    28081 tatttctgca tcatagtatt gtatgaaacg ttcatatact tagcagcttg tgccttgttc
    28141 atatattttg gaaactggta ctttttttct aataatgaaa gtaacgttcc ttgagtttgc
    28201 tctacttgtt gaacaactcg atcgtcaata attttaaaag ctggatcatc aagcgtgagt
    28261 ttgtcaattt ttaattccat atttatcccc cctcgttagc atacatttct atacaaagat
    28321 tttaaaaaaa tgcaagaggc tcttgaatac atttctatac ctcttataaa ctcaatatac
    28381 catcatacct tttcatttgc aagaatttta acatactttt ttgaactttt atgtttcaca
    28441 catatttaaa tcgtgatata gtagagaaaa tatatgaatg gtggtgaaaa tgtggctaga
    28501 aaaaaatacc ccgaagcagt ggagacagga caaagaatta gactggctag aattgaaaag
    28561 ggactctcaa tggaacaact tggaggtaaa ttatccccac cggccagtaa aggtgccgtt
    28621 agtaattggg aaaatggcta taatcttccc aataacacca ggcttaaaca actttcaaag
    28681 gtattagatg tatccactac ctatctatta aacggatctt atacattaag agatatgcat
    28741 atgatgcccg aaattaagcg aaatgtgctt gaaaagcaat tgaaaaaaac tattgaaaaa
    28801 aataactcta taattgattc taatctttct aatgctatca ataacatcga agaagataac
    28861 ctgactgtct cacagaagat ttatttaaca aacgcaataa aatatcttaa aacaggatct
    28921 cgaattgaat ttcctgacgg gagtacccca attgcttatc tcgcagctct tctaaatacg
    28981 cttttacgat atgactcatt aattaatgat tcagaaaaaa atacagatga gaaaaaagag
    29041 tatcaagaat tttatgaaaa tgatcttcta aaagagttca cagctcttgt tactgagcta
    29101 aataaacact attcaaaata ataataaact gcctcctcca accgccccca cggcttgcta
    29161 cactatggag ggaaaactat tatggcaaca ttcaagcaat atgaaactaa aaaaggtaaa
    29221 ttttggctct atgaagccta tcttggaatg aacaaaatga ctggcaaacc tgataaggtt
    29281 cgtagacgag gttacaaaac aaaaaaagaa gcccagcttg ccttgtcacg cttgcaagtg
    29341 gactacgata aaaacggtat taaaaaagcg aacaacgaaa cgtttaaaga agtttatgac
    29401 ttgtggctag aaacatataa aaccactgtc cgagaagcca ccttcatgaa aactgaaatc
    29461 aagtttagaa agtggattct tccaaaatat ggaaatcttc gtgtgaatga agtcacggta
    29521 aaaaaagccc aacaaattgt aaatagctgg gctgaaacta ctgaccaata caaggtactt
    29581 cattcaactt ctaagcgtat ttttagctac gctatcaatc ttgggattat tgagactaat
    29641 ccactagaca acgtattaat gcctaaaaga accaccacta agcataaaga aaaggtcaaa
    29701 gtttacagta aagaacaact gatcaccttg tttgattacc taaacagcaa acctgaaact
    29761 tatcgaaacg actttgataa aacgttacta cgctttctct tttatagcgg ggttaggatc
    29821 agtgaagccc ttgcattgaa ttggtcagac attgattttc aagaaaatac ggtcaccatc
    29881 aataaaacac taagccaatc caaaaatggc tataaaatca gtgatcctaa aactgattgt
    29941 agtgctgggg ttcttccact agatgaaaga acaatttcta tattaaaaaa atggcagatt
    30001 aatcagcgga aatatatgct gacacttggt gtaactgatc cgactatgat tttttgtggt
    30061 atctataaag aactaatcac acaccacgct atttatgcaa gattgattac tatttgtgaa
    30121 aaagctatgg ttccatttct cggtgtgcat gtagcaagac acactcatgc ttcaatgctc
    30181 ttggacgctg gagctactat ggtagaagtt caaactagat tacggcatac aaaaattagt
    30241 cagacgatcg attgttacgg tcacttggca aaagaaacga aagaaaagac tgttgaaaaa
    30301 ttagtacaac acttaaatat tgactagatg gcatacaata tggcatacag taaaaaataa
    30361 aaaaaggaga aaccctatca tatcaaggtt tctcctagct ttatagtcta ttcttcttct
    30421 tccgccatga atgtattgaa tacttcttca atcatgtccc attctgcgtc tgtttcgatt
    30481 tgttctaact cgccttctgt tccatcttcg ttttcgatgt atgcataagc ttgtaattct
    30541 acatcttcat cttctgaaac accagcagga tataaaagaa catagttacg tccaaattct
    30601 tcttgtccgt caactgttag taggatttca tataatgttt catttccttg gtcatccacc
    30661 aatgtgatgt gttcgtgtcc ttcatggtcg tgggaatggt tatgttctgt cataagatta
    30721 cctcttttct tttattatta aaataattgc ttacctgagc cgtctaaata attttgtaag
    30781 atcatcacag ctgctaattt atcgatgact ttttttcgtt tggctcttga agtatttgcc
    30841 tgctctacta gcatacgttc tgcctgaaca gtggttaatc gttcatcttg ataatcaact
    30901 ggcaaggaga atagttcttt gatcttttct ccgtatgaca tggaagcttc tgcccttggt
    30961 ccgatggaat tgttcatatt tttaggtaat ccaactacaa accggtctac ttcatattgt
    31021 gctactaatt ccttcaaccg atcaaagcca aactcttctt ctgcttcatt gatccgaatg
    31081 atctctactc cttgagctgt ccagccaaag gggtcactga tagcaacacc tactgtctta
    31141 gaaccgacat ccaagcccat tactctcata aattcactcc atgatttgag aggtagtcct
    31201 tgacaattac ttccatgatc tcatcgcgtt cgtgacggcg aatcaaattt cgagcatcac
    31261 gataacgagg aatgtaggcc gggtctccag aaagcaagta gccgacaatc tgattgattg
    31321 gattgtagcc tttttcttcc aaagcccgat aaacaatcgc taatgtttcg ctcacttctt
    31381 ttttgcgact atcgtcaaaa ccaaaacgta cggtttcatc tgtaaaaccc atgattctac
    31441 acctctttcc cgcaaggtaa ttccagctta ccttctacaa ccttatttta ctcgaagaaa
    31501 gggaaaacta caaacaaata ttccaaactt ccgaataaat taagaaacac tatttgtatg
    31561 tttttttcct tttttctgta gctttatcaa aagtactctt ttcataaagt atacgctata
    31621 tttctttgaa tgtatgtttt tacacaattt tttcataata ttcataaaaa attcgaatag
    31681 ctgctactaa ttttttgttt ttgttgcttg tcctgtctca taattgatcg tgtagccatc
    31741 ttgaacattg tagataaaga cattgaattc aatttttttg tcttcaatgc tttctgcttc
    31801 caattgcact cccctggcta ccagttcatt cccctcaaaa tgaggagtga cacggtagcg
    31861 tacatgatga tttgtttctt tgatatatgc cgcaatatca ttttcgtgag caagcatgta
    31921 tggagcattc aacgaacgtg tccctgtcat caagttttta atgttgttat tttgaccagt
    31981 cagctggaaa ccaattaaat ggctgcgatt atataaccat tgcccgtcac ttaatttctt
    32041 ttgtttccaa cctgtcgggt caatatacaa aggctctctc ttttcagtag ggaacaaatc
    32101 ttttccaagc atagcattgg cttgtcctac tctgttcaat ttatcaatat ttgaatagct
    32161 ttcccatgtt cctttggaaa ggctaagatc ctcttttgta aattccggct gattcccatt
    32221 gatttctatt tcttgatgtt ttccatcata ttctggcaca tccgctactt gagaagcttg
    32281 tggtgtggaa tcaaaaatag ttgtaatctg ctgaaagata tctgaatctt tttgtgtggt
    32341 ggaaacaccg aatgcccctg caatcacagc agcgacgatc cccacgatcg ccttggttgt
    32401 ttttttgctc atttctttac tcctttacta acagctccct aaatcttaac atatgttccc
    32461 ctataaataa atactagaaa gaaatagaaa cagcaaaaaa taaaacctgt tttaagatgg
    32521 cttaaaaata caaaaaagag acggattcct cgtctctaat cagtcaccac tctatctgta
    32581 aggtatttat tattattcaa aaattttctt catactcaag acaatacact gatttcacaa
    32641 ccgcttggta aacggtgcgc aaaagcttgc atctgcggta ataagtccat ccaagttaaa
    32701 aacataagaa gttgtttttg cttggctgtt cttattactt gttgcagagc agctttttca
    32761 caacctattt taagcgggtg acgggaatcg aacccgcgac atcagcttgg gaagctggga
    32821 ttttaccact aaactacacc cgcaatacaa aaacagtata cgcctctttt attccttcct
    32881 caagtttttt tattctttac gcttctggta tgttttgtag taaaataaaa ctaatcaatc
    32941 aattattgga gaagatgcta tgacgggaaa agaattagat ctgttgctgg acacttgttt
    33001 actcgctggg aaaatcatga tggaaagcaa tgcggagatg tatcgggtag aagatacgat
    33061 gagtcgaatt gctttagctt ctggaaattt ccgtttggtc agctatgtca ctcaaaccgg
    33121 tctctttatt ggtcttgatc gtacctcgac catccgaatg gaacaaatta ccaaccgttc
    33181 gattaattta gaaaaagtag taaaagtaaa tgatctttca cgtaaatatg ttgcttcaga
    33241 gctaacatta gaagaacttt actgcgaact gcaagcaatc gaccgagacc gttctttctt
    33301 tcctgtctgg ttgcaaatcg ttagcgcggc agtgatcagt ggaacaatta tggtgttatt
    33361 tggcggaaag atgaccgatt tacctgtgac tttagtcatt ggaggattgg gatacgcagc
    33421 ttatctatat agtttgaaat tttttagaat caaattttta tcggaatttt tatcttcttt
    33481 attgattggc atagccgcta tctacagcgt acgttttggc ttaggaagca atcaggatct
    33541 aatcattatc ggatgtgtga tgcctttagt tcctggagtt caaattacga acgctatccg
    33601 tgatttgcta gctggacatt atgtctcagg agtttctcgc ggaacagaag cgatgatgac
    33661 tgctacgatg atcggttttg cgattgcttt tattttccag cttttttatt aggaaaggaa
    33721 cacaagtatg gggacgatat ttattcagtt ttcttttagt tttttagcga ctgcggcatt
    33781 tgccgttatt acaaatgtac ctaggcgttc attgatttgt tgtggtcttt ctggtgcttt
    33841 tggctggatg atctactgga tctgtgttca aataggcgga tctgctgctt ttggttcatt
    33901 gcttggttct ttaggagtag cattcatcag cgatcttttc tctaaacgtt tgaaaatgcc
    33961 cgtaacgatt ttcaatattc ccgggatggt tcctctagtc cctggtggtt tagcttatca
    34021 agcggttcga aatctcgtta ctgaatccta tcgagaagcg gctcattatg cggtccaagc
    34081 aattatgatt gctggagcaa tcgctttagg tcttgtatta tcagaagtct ttaaccataa
    34141 catccgtaat ttcaaagtaa agcgtgacgc aatcatgatg aaacgaaaaa aacggctttt
    34201 taaataaacg aagtgtaaca aagaaaaggt tgtgaaagag acgttcagcc ttgagcgatg
    34261 acaaaatatc tcacaaacat tgcgacaagt aaccgcagga gcaataagaa gtaattatga
    34321 gtattaaaaa aagttttgta aaaatagctt ctcacatttt tacataaaat tgacttaata
    34381 ctgagaaact gacttttgaa caccctctat ctgtaaaaaa ggggctgcga catgattttt
    34441 gtcacggccc ctcttcttta ttcaaatttt ttccaaaacg ttcgttgata ataaacgatc
    34501 catagagcgg aaagtattag aactaaaccg atcacaggaa tattcactaa ccaaaatgga
    34561 taaaacaaag ccaacattcc gtataagacg ataagaataa tactgatgat caatgaaccc
    34621 atcatagttg cgactaaacc caaacttcct gaacctctgt taaataattg gttaatattg
    34681 gtccagttca gcagcagcaa ccgataatct ctagcgaaat aacgtagtga caataagtag
    34741 cagccgaata aagaaccaaa tagcaagcta gggataaaaa acaaaggcag ctgaaaagac
    34801 agtccgctta ataatgcgat cccaccagtt aaaaaactct gcaatatcca tccgaatctg
    34861 aacttttctt ttaaatattg gttcatagag attggcaaag aacggataaa cagaaaattt
    34921 tcttgatcca aagaaataag attgctgata aaagaagtct gattaactgt taagaaagcc
    34981 aatgcgatcc ctaccaggaa aacaacaccg attaacttat tatctaaatc agttaatttt
    35041 atttcgcctc ctagggcaaa agcaagaata aaaatgattg gagttaacaa ggaagaagaa
    35101 agaacttgca taatcaaatt aggttctttt actaattgag cattgtaact aaataaaagc
    35161 tgatgcacat tttggttctt tttatgggtt ctttttattt tgaactcact gtttgaatca
    35221 gtcagttgct ctgtaagctt tggtactatc agctgtttta cagcaaggaa aaatcccaga
    35281 gccgtgatag ctagtattcc ccaacccaat aaaccttgtt gagaaagtgg ctgactggca
    35341 gcatcataga atggcaataa tatcgaaatc ggatgccgat ctgttaactc attttcaata
    35401 gtccccgttt gatgattcat ccacatgatt ccagcaaatg cgatcagcat cgagctgcca
    35461 agcaagatac tagaaactgt ttttctatgt tttttaaaca ctgctgttcg agccaaacca
    35521 aaaacaatca aactagccag acaaaaaaga ataatcaaaa atagagaaaa caaaaggatt
    35581 gagataatga tagtaagcaa aatccctaga tcagctcgcc atccggttaa aataaacatg
    35641 actagaagcg gaaaaacaaa aggaatgatg gtcaaagcaa ctaccaaaat ctttgctgaa
    35701 aaaacctgtt cttgtccaaa aggcagaggc aaaaatactt gcaggtcatt tccttcaaaa
    35761 aagatattat aaatgactga tatactttga gttaaaccta acataccaaa aagtgccacg
    35821 taataagtaa agaaacctgg caatttaaca aagttcatag caaacatagc aaatccgtaa
    35881 atcagtaaaa aaatcagccc tgaaaaaaga ttttgagaca aaagatagat agttaatcgc
    35941 tttccctttt ttcctttctc tcttgcttta ttcgtgagct gtggattaac atataaaaga
    36001 ttaactcgaa ttaattctaa taattgtcgc ttatacatac ggtccatctc ctgtcgcttg
    36061 ccttccagcc attttcaaat aaatcttttc tagcgtttca tcaggtgagc cattcaataa
    36121 ttcactaact gccccctgat aaatcaattc acctttcttc aaaatagcca gtttatcgca
    36181 taactgttgt gcggtatcca atacatgtgt agaaaaaatg actgtttttc cttttgcggc
    36241 atgagccttc atcatttctt tcaaatcaaa tgctgcttga ggatcaagtc cttgcaaagg
    36301 ttcatccaat acccaaatat ctggatctgg cagcaacgtg ccgatcaaaa tcgttttttg
    36361 acgcattcca tgtgaaaaac ttgcgattgt ttcattttga tgactataca tatcaaaaag
    36421 cgctgtcaat tctgctaaac gcttttcttt tttttgtgtg tctaattcat aggcagctga
    36481 aatcaagtcc caatattctc cagccgtcaa ttgtaaaaaa atatctgggg aatcaggcac
    36541 ataaccgatt ttttgtttga tactcaatcg attttccgtc aatttcattc catctactaa
    36601 aatcgttccc gatgatggtt caataatact aactaaactt ttaatcgtag ttgattttcc
    36661 ggcaccatta tgtcctaaaa atccaaaaat ttccccttgt cttatcgaga gagacaagtc
    36721 ttttaatgct tgttttttcc cgtaactttt acttacattg atgaattcaa tcatttctgt
    36781 actccttttg tttgttatta ctttttattg tacccttcgt tattggtgga gtaaatgtca
    36841 aaaagagtgc ataacaaaaa aggatagaaa cgactaggct gctacttggg ttgttctctt
    36901 ctacaggaaa acactcttct aacaggtaag atatgagtct ttgttttctt taaaatgatt
    36961 cattttctat cttttcttgc ataaaattct ttagtttatc aatacttctc tttcccatct
    37021 ctagttgttc tccatttgca aatgttacta tttcgtttac tctgctaata gatgtaatgt
    37081 tttcagtatt aatgatataa gcttttaaat ctttacataa atactcatat tcgagtgatt
    37141 ttttatattt tttaattgtg ccgtttacga gaatctcttc atctttcaat actaggccta
    37201 ttttattttt ataacctggc atgctcatta gataaaggat ttcatcgtaa tggatatagt
    37261 gatcgctatt accataacgg acagttgtcc accttttttt cgtagcatgt tgaacaggat
    37321 agttttcttt ttctttcaat aagtaacttt ggatgctttt tttatttttt tcataagtga
    37381 attcttttat caaataacca ctaggcgtaa tctttcgatt aataacagtt attgctttat
    37441 tcggatgaga cgttagataa acaattttac acttttcatt cttttctcta agcatttcgc
    37501 ctagttcgat accgctataa taggcgttca aatcgatgtc taggaaaaaa atatctgagg
    37561 aatagatttc attattttta atgaattcaa caaattgtat aacgttcaat ccctgaacga
    37621 tctcaaattt ccaaggactt aataaaattc tggattcttc caaaatttct actagcattt
    37681 tttcaaaaac caaactatca tccaaaataa atatacgcgt taccataaaa agtacctcca
    37741 ctcgatattt tactatcaaa atcatttcat acaacttttc aacatacatg ttaggaataa
    37801 attgtaattc tcttcacaat cttttccaac acctttgaaa ccgatatcaa atactttcag
    37861 gatgctgctt ctaaaataaa tatcaaacaa atgaatacat catgcaaaaa aagccagaca
    37921 accgacgtcc ttgcgtgtca gctgtctgag ctatgtttat ttattttgtt cttttaaaaa
    37981 gaacgataca catactgatt tttcggagca gagactttct cagcgtttgt aacaactaca
    38041 gatgggattt cacgcttgaa tggttgataa tagtctgatt ggatggttcc ttctatttta
    38101 taccattcat catttttaac cgttactcct tccggcatat gcgtcaacaa accaaagact
    38161 cctgaatctg ccacacaatg gataatgccg aatcggaaca agaagatatc tgtattgcta
    38221 tcgttcggtg tttggtaaac aaatccttca tagctgattt ttttgccaat gaattcgcta
    38281 gggtaattat aaattagctc cataacttct aaatagtttt catctgtgat cttgatagtt
    38341 tgcttatccc catacttttc tttcaactca ttcatttggt ctaagtagtc tgatttgttg
    38401 aaataaatac tggtatccgg ctttaagtat tgtgtctgca tctccggatc ccctactgat
    38461 tctttactta ctgggaaatt aaatcctttg gcttctacga tcgtagtgtc gagactaact
    38521 gttggaaaca acgtccccac gattactggc aaagctagca gtacataagc gattccacgt
    38581 tgataaggtt ttgacaaccc atggtcatga tcgtgctcgt gtgccttttc ttctgctttt
    38641 ccgtttttga cccacaaata cagctgaact aaagccaaaa gagcagataa aatcattgaa
    38701 agaatagcta agtagcgata atggacattg atatattgat cgagtttgcc agaaacttgc
    38761 aagtacatca tcaattcgaa atacccagat aaaatcaaaa accgcagcat attttccctc
    38821 ctaaataaat aatgtgaatc caataacgct aagggcaatc agtccgatca gcgataaaat
    38881 aaagcgtcct gtaaaatatc gtttcatcat cagtagattt ttgatatcga ccattgggcc
    38941 aaagaccaaa aaggcagcta caggtgctgt gccgaacaga cttaacaaag aagatcctat
    39001 aaacgcatct gcttctgagc aaagagacat cgtaaaggcc aataacagca tgatgataat
    39061 ggctaaaatc tttgttgagc cgagcgttag cataattcta gttggtaaat aagtctgcat
    39121 cgcagctgcg attaatccgc caatgatcaa ataacgacct gtatcaaaaa attcgtcgat
    39181 tgaatgtgtc aacacatgcc cagtttgatg gatcagctga ttttttggtt cattcttctc
    39241 actttgttct tcttcatgga catgatgatg gtgattttct gctactttag acgtgatttt
    39301 ttctttcaat acagcgtctt tttgaaaata ggcaagccag acaccaatca caagggcaac
    39361 aaaaaagctg cctatcaccc gccagcttgc catctgccaa gaattgccaa atgcgataaa
    39421 agtggaaaaa atcacgattg gattgatgat cggagcagtc agcataaaag caaacgctgt
    39481 gtgaactggt acgccttttt taacaaattg atgaacgatc ggtacgatgc cgcattcaca
    39541 tgaaggaaag aaaaatccta agatcgaacc agtaaaaata gagaagatac gattttcagg
    39601 aagccatctc ttcacacgtt ctggggtcaa aaatacttgt aaggcgcctg agataagaca
    39661 gcctaataat acgaaaggta atgcttcaat aacgatcgat aagaaaatcg tactcatctg
    39721 caaaaccgaa tttggaataa aagaaaacat gagtttcctc ctttttattt ctagttcatt
    39781 cgctttgcaa atatacgctt ttattcaaaa aaaacaagtt gctctgtaaa tttttacaca
    39841 actcttattt tttaccaaag cacttcaatt gactttttct gttattccca ttaaattaag
    39901 gtctcataca tatcacttct atcataaaag ggagaataaa aatgaaacaa accactaact
    39961 tgactgaagt tcaagacata ctgcaacagt catccgttgc cgtcatttat ctttcgatgc
    40021 ctaattgcag tgtttgtcac gctgttcgtc cacgactaga ggaacttctc acacattatg
    40081 atattccggc acttcatttg gatgcatttg aaacaccaga agtagctagt aaatttgaag
    40141 tgctgactgc gccagttgtc ctgatttttc atcaaggaaa agaagtcttt cggcaagcac
    40201 ggttcatcga ctttaaaaaa atccgttatc tactagatga actgacagca gaagatgatt
    40261 ccctttctta tgaagaaatc ttccgaacaa aataatcgtt gtccaaacag aaaaaagcga
    40321 ttgaacaagt gaaagttttg ccttcatttg ttcaatcgct ttttattttt attctgctgt
    40381 attttcggaa tgcgcatttg cgtcagaatg caagaaatag atcaaggttt gcaattcatt
    40441 cgtcaaatca acattttgga tcaatacatc tgacggagca ttcaatcgag cagcggtaaa
    40501 gttcaagatt cctttgattc ctgcttccgt taactgattg actacttctt gtgctctttc
    40561 agcgggtatc gttaaaattg ctacttcgat ttgttggatc ttgatttgtt ctgacatatc
    40621 ttccattgga taaacgggga taccgtctac gatgcgccca acgatttcct cattcacatc
    40681 aaaagcacaa ctgatacgta tgctattact ttgatggaat ttgtatttta gtaaagcact
    40741 tcccaagttc cctacaccga tcagcgcaac atttgtcaat tgatcgtcgt ttaatgtctt
    40801 tgcaaaaaag tgcataagat cttctacatc ataaccatac ccacgttttc ctaattcgcc
    40861 aaaataagaa aaatctcgac ggatcgttgc actatctact tgaacggctt cacttaattc
    40921 agttgaagac acttttttct ttccagcatt atttaaaatc cttaaatagc gataatacaa
    40981 aggcagtctt ttagctgtcg cctttggaat aatatgatct ttcacgaaat gacctccacg
    41041 tattgtctaa ttacatcttt gatttctcca tcatagcagt atttatagga aaaaatcaat
    41101 tccattgctc agatattgtg aaattttatt ctttattcat ttatttatca tttctgcaaa
    41161 tctcgcagta taatggaatt tatggtagac tactaattga aaaatagttt tttatttgag
    41221 gagaattcac aaagtagaaa agaggattcc atgatattat tacaagccaa tcagattgct
    41281 cgccattttg gtgcagaagt cttatttgaa aacatacatt tagaaatcag tacaggcgct
    41341 cgtgtcgctt tagttggcag aaatggcgct ggaaaatcga ctttattaaa gatcattgct
    41401 ggaattgaag cacctgatga aggaaagatc gctaaaaata aaacagcgac tctcggatac
    41461 ctcgctcagg acacaggact tgattccaat gaaacagttt ggaacgagat gctgaaagct
    41521 tttgaacaag ttagacaaat ggaagagcgg atgcgcgagc tagagatttt cattagcgaa
    41581 ggtacgccag acacacctgc ttatagttct tatttaaaag aatacgaccg gctgcaacac
    41641 gatttttcag atcgaaacgg ctatggttat gaaaatgaaa ttcgttccgt attgcatggg
    41701 tttaaattcg attcttcatt ttatgatcag aaaatcgata ctttatcagg cggacagcgc
    41761 acacgtttag ctttagctcg catgcttttg caacaccctg acattttgat tctagatgaa
    41821 ccaacgaacc atttagatat cgagacactt tcctggcttg agaattatct gcaaagctat
    41881 actggtgcat tattgattgt ttcccatgac cgttattttt tagataaagt agtcaatgaa
    41941 gtctatgaat taagcagaag aaaaatgcgc cattacaaag gtaactattc gaaatatcta
    42001 gatttgaaag cagaacagtt agcaagtgaa tggaaggctt atgaaaagca gcaaacagaa
    42061 atccataaat tagaagactt cgttgctcgg aaccttgttc gcgcctctac cacaaaacga
    42121 gcgcaaagcc gcagaaagac attagaaaaa atggaccggc tggatcgtcc tcaaggggat
    42181 gaaaaacaag caaactttct attcagtata gaaaagcctt ctggaaatgt agtcttgcaa
    42241 atcgaagatg ccgctatcgg atatgataat gtcaccttat ctgaaccaat caatctagat
    42301 atccgtcgtc aagaagcgat tgctctagta ggtccgaatg gtatcgggaa atcgacgttg
    42361 ctgaaatcaa tcattcacca gatccctttt atccgtggaa aagaaacgct agggacaaat
    42421 gtcaccatcg gctattacga tcaaggtcaa gctgatctgc atacaaacaa gacgatccta
    42481 gcagaattgt gggatgagca tcctaccact ccagaaaaag agatccgaag tgctttaggt
    42541 gctttcttat tttctggcga agatgtagaa aaaccaatcc ctctgctaag tggcggtgaa
    42601 aaagcacggg ttgctttagc caagttagcg atgaacaaag aaaatttttt gattttggat
    42661 gaaccaacaa accatttaga tattgacaac aaagaagtat tagaaaatgc attgatcgat
    42721 tatgacggca cactgctttt tgtttcccat gaccgatatt tcatcaatcg gatcgcgaca
    42781 aaagtcgtcg aactctctga aaatggcagc aaagtctatc taggagacta tgattattat
    42841 ttagagaaaa agcaggaaga agaagaaatc gccgaattgt tggcacaaga tgagccagaa
    42901 tctgctcctg ctcccacgaa caaatctgat tattaccaaa gcaaagaaca gcaaaagctt
    42961 atccgtagcc ttcaacgaaa aattactcag gttgaagaag aaatggcgcg gatcgatgaa
    43021 tggatcgatc agttggaaca agaaatggtc gcgccagaaa acctggaaga tcacgtaaaa
    43081 ctcaatgagc tgaaccaaga tttagaagct gctagacaag atcaagaaac aaaattgacg
    43141 gaatgggaag aactaagctt ggaattagaa gaatatgaaa attaacgaaa aaaccaaaac
    43201 agaaaccatt catgctgttt tggtttttct ttttttattt tatcaaacaa aaattccata
    43261 tttacctttt tcaccaataa gaagaccaat tactagtcat tctcactaaa gcttctaaat
    43321 aatagtaatc accccaaata caaggtgtat ttaccccatt atttttgggc ttattataaa
    43381 cagactgtgt taaaaagcca tcttcttctt ccgttgtagc atatctttct ttcaattgtc
    43441 gcataatttt ccaaacagca gactggtata agttcttttc tgttttgtct aatagacctg
    43501 attctattaa ttctaataat ccacaaacca tgatcaccat actagatgta tctttttctt
    43561 ctctgctaga atcatcaaag atcaaatccc aataacaaat ggtatcttct ggcaaccgat
    43621 tcataaaata attggctaat attttgctta aatcaaaata tttttgttct ttcaaataac
    43681 gataacttaa tgcaaaaccg taaatccccc atgcttggcc tctagcccaa caggaattat
    43741 cactataacc ttgatgcgta cttccatatc ttggttttcc atctttgaca tccatataaa
    43801 atgtatgata agttgaacca tcttctctta ctaaataatc tgctgcattt tgaatatgag
    43861 cctgagcagc ttttttatat ttttgatttc cagtttcttc actcgcccaa tacaataatg
    43921 gaagattcat gcaacaatcc atgatcattc taccttgttg tgaaggatca ctaggatctc
    43981 cccaagcttg tataactcct gccttctccc aaaaacgctg aagtaatgtt tctgcagcat
    44041 caattgaagc ttccttcatt tttcgatttc ccgttatttt ataatctgct acggttgata
    44101 aagaatacaa aaaaccaaga tcgtgatgat tagtgataat ttgacagtca atcctgtgga
    44161 taaaatctat atctgctttt tcagctgttt gttgaaataa aggatcaccc gtcttctcaa
    44221 ataacaacca attaattcct gtccaaaatc cttctgtcca accaatattg tctcttaatg
    44281 gataacgtcc ttttatagga cactcgcttg ggaattgata ctgactgtta ctgatacgtt
    44341 tgattaacct ctccgtgtgt ctgcgacaac gctcgatctc attattccat tctttttcca
    44401 ttaattcttt ataacattca tttatccatt tttctggatg tattatctct tcaaacatca
    44461 ctttatcaag catttcccca ccagccttta gcataatttt agtctttttt tattgttttt
    44521 attttggata gttagcccgg ttgtcccatc aaaatcaatt ttgggacgat tgttccatac
    44581 ttctaaacta taatcccgat cttgatgagc aaccactcct gttccgagcc aatgggttcc
    44641 ttttccaaat gtaccctcta atccaggaat aaaagtgttt tcccatgtca tcaagtttgt
    44701 attgggcaaa cccataatag ctgtggattt tcgtttaata gtatcgtcac ttaaacagat
    44761 aatacctgaa aagccagctt cactcaccct atagttttcc ttgtcttctt ctatgattat
    44821 tccttctttc tcaggagaac attcaatagc aaaccctgtc tcatatagaa catattctgt
    44881 atctaattct actttgtgta cgcgaatctg ccagccttca aaaggaataa gccaagttct
    44941 gatttttgcc tgtaaagtag gttcccatgt agaggcaagt acctctttct tacatacaat
    45001 gtctcgtaca ttatttcgaa cgatgaactg tccataggtt ttgatgctct cattcttaaa
    45061 actggtcgtg atatatgtgt ccgctcgctg aatccctagc attgaatcca ttgccatttc
    45121 tgctttcaat tgattcgctc gcggtatcga aaaaccaaat tgactagaat aagcaaattt
    45181 taaatatttt tcattggtca atgtatggaa atttggccct aattgtcccg cattcaaaaa
    45241 aaatgtatgt ccattatcat gttgtatgag caaattggct tctgaaagaa gtttagttcc
    45301 ttcacgaata ggcataggca tttctttagc cgtccaatac tcatggtctt caggtagcgc
    45361 caacaataaa aaaatcttat tcagccataa tggagataac gtactattat agggttcagt
    45421 caatgtcaat tgagggtagg tataaccaat attcaggata cctttttcat caaatatttc
    45481 tttttgcatc caccatctta tattccgatt gactaacccc ttgatttcac ctaatttaaa
    45541 aggcaaaaga tcggcccaca acatagctga ccaaaagctt gaaactgcaa aacgataaat
    45601 catgcttcga ccaaaaggca catttgcacc atcttccgca aaaaaatgaa tatagtctct
    45661 agaaaaatca atagctcggt tcttatatat ctggctaatg ggcttttctg gttctagtac
    45721 gctataaatc aaaccataat aatgaaatgc aaaaggatta taataatctt ggcgacctct
    45781 tgaactgtct cgataccagc cattttgaag ataacaattt tctatattat caaatgcttc
    45841 tttcagtgct gattctttaa ctggataacc aaatttttgg ataattgttc cgacaattat
    45901 cttaaaaaat tgccaattac catctgcaca ttcatattca aaaatttgcg agaaccaatg
    45961 aataatctgg cttttttctc ctgtatctaa ttttttccat aataattgtt catgatgata
    46021 taacgctaat aacattgcgg gcatttctac catacgctgg tctctttcat gtatttctcc
    46081 ccaataatcg ggatgggaag gatctgttcc agcttttatt cctgatagaa ttaatttcaa
    46141 ccatcgttca tctaattggt caattgcggg ccccgctccc cataaaaccc gagagaatcc
    46201 ttccattcct gcaattttat ttccatatcc taccccttga tgtatataat caatttgtgc
    46261 gccacttgct cgataaaacg gctggagtgg ttgatttatc tttttaaata agtcaaagta
    46321 atcctgtctg gtccttaata gtggtactgg atgtttcatt gtattttctc ctctttttac
    46381 taccaaatag ctaattccga ctgttctaat cgaagtaagc cttcaataaa aaaataatca
    46441 gaatagatca gtggcacatt ataagtcagt tctctatggt atgcttctgc tccgcctcca
    46501 atgatcccat cttcatccat attccaattt gcatagtgct tttctgtctt ttggagtata
    46561 ttgatagcag cttcatgata catttttgat cgtacagagg aaacatatcc tgctaattcc
    46621 agaagaccgc atgcagcgca tactccagca ctggtatcaa tattcgaatc ttttacagca
    46681 aaatcaatga gaggaatata accagttttt tcgattttct gaataaaatt tactgctatc
    46741 ttttctgcta tagctagata gtacggattt tttgtgtaac gataacttaa cgcgaatcca
    46801 tataaagccc atgcatttcc tctgctccaa caagaattct cattgtatcc ttgcccgcca
    46861 agtaaccgaa tatatttccc tgattctgca tcaaattctg cgatatgccc ggttgatcca
    46921 tctttcctca ctagatactt agctgttgta tctgcatgct gccgagcaat tttagagaac
    46981 ctcggatcac cagtttccct cgaagcccaa tacagtaatg gtaaattcat catactatca
    47041 ataatcatcc aaccaggttt atggtcattc catgctacta aatagttccc tgaaatatta
    47101 tagcgtccag caagtaccgt agctgcatgt aaccctagtt gtcgactatg tttgttttga
    47161 gactgttgat aatgtgcaat agccgatggt aagaataaaa aacctacatc atgatgaacg
    47221 tctgaaaaat ggtacagggc agattctaat aattgttctt gttgtattgc tgcctctttg
    47281 aacttttctc gttttgtctt gtgaaagagc tgccataaaa taccactcca aaaaccattg
    47341 gtccaccagt cgattcctct gtcctttatt acgtctgtat attttccgtt gtaagaaata
    47401 tatggaattt tattccccat tctgtctaat tccgcatcta tcttttcaga agctacattc
    47461 aaaatttttc ttgaccattc cttcgtctgt tcattcatgg atatcccgcc attcttttaa
    47521 tttttatgaa tgtggagttg ctcctagaac gaactagatg gttaacattc gttatttttt
    47581 ttacttattt gcagctccaa tgtcattctc cttaaattta gactgcgccc acacataaat
    47641 caagaccatt gggatcactg aagttgctaa aattagcata attgcgccat aattaattgg
    47701 ctcacctgta ctagaaaccg tcatattttt gataattaag ggaaccgtat atttatcttt
    47761 atctgaaagc atgactaatg gaaatagata ttgattccaa cttcccataa agataagtaa
    47821 accactagta ataatcgtag gtgataaaat cggtacagcg attttcgtaa aaatcaccca
    47881 ctcattggca ccatcgattc tggcagcctc catcagtgct gtaggaaatt gactgctata
    47941 ttgtcgcatc ataaacactg aagttgtact ggcaatcgaa gggatgatca cacctgcata
    48001 agtattcgat aatcctaaac gactcataat gtaaaataaa ggaatcaatg tggtaaaaaa
    48061 aggcaccatc ctagatagca taactgtatt gaacaacagt ttattcccca taaaacgata
    48121 ctttgctaat gcataacccg ccattgtaga actggctgta gctaaaactg tactgattac
    48181 agcaataaca atcgaattcc aaataatcaa gggataatta tatctcgcca tgatggcttg
    48241 ccagtttttt aatagcatat ccccaaactt gaaattgatt ccgccactta aaatttcttg
    48301 attactttgc gttgaagcta caatcatata taaatatggc atcaaaaata ttaaggcacc
    48361 aaacacaata atgacgtacg taaaaattct gctttgtatc ttttgtttag tcatttttat
    48421 cacctccgaa tttaaattga atagccgata atataacaga tacaaacacc atcgtccagg
    48481 caatagcaga ggcttttcca aaatcaacca tttcaaaact tgttttatat aaattgactc
    48541 ctacgaccaa agctgaattt tctggtccat tggaagtccc aaagaatata tttgggatat
    48601 tgaaattacc caagccattg atagtggcta aaactgtggc cattaaaatg attggcttta
    48661 gcatggggat cgttaataaa aagaattgcc gaaatttcga tgctccatct atttcagcac
    48721 tttcgtatat ctctttagaa atcccttgaa gaccagctaa aaataaaata gtgttatatc
    48781 ctacactaag ccaaatcatc gtgatgatca ttgatattcg tatccaaaaa ggatttgtta
    48841 accactgtac accgttaatt cctagcgcat tcaaaaatcc attgaataac ccagtcggat
    48901 tgaaaaaagt catgaatacg gaagctacag ccaccgttga tgtaatggtt ggaatataat
    48961 agacagttct aaaaaatcct tttcctttta actgtgctga attcaataaa acagcaaagt
    49021 aaatagctaa tagcatcgtt attggaattg atcctgccat gataatcaaa acattcgtgt
    49081 aagatttaat aaacaatgga tctcggaaga tagctagata attgtcaaat ccaacaaatt
    49141 caaatgagta gccaattttc ataaaactaa aagcaaacga gataatgatg ggaatcaaaa
    49201 tcgtcacaat aaagaaaata acaaaaggtg tgaccaatcc ataagcactt tttttatgag
    49261 acgtatctgt tgcttttttc ttgctgttct ttattgtctt gcttggtaac gaagattgca
    49321 tcatcgttaa gtcctcctaa tattttttaa tcaccagtta attttacgcc gtacttatga
    49381 gcaaaatttt ctgcttcttt tagatatgaa ttttcaaaat cacctgtttg ccaatagtta
    49441 tatgcagcgg tagtcaaata agacgtagca tctgaataag tggctggtaa aacagtattt
    49501 gatgtatttt cattgatttt attcaatagt tcatagtatt tttgtcctcc ttagtagtca
    49561 aactttttct cagctgcttc tgtttgatag gccaatgagt tagatgtagc cacgcccagc
    49621 tccagtgcgt tggctaagca ttcttcatct gtcagcataa atgtaagaaa ttgttgtgcc
    49681 acttgtggat ttttggcatc tttagggaca taaaaacttg atcctcctga tactgcacct
    49741 tttcctggat tttcttttga aaaaggaatc agtggagcca tgcgccattt gtcagatgca
    49801 tcaggaaaat tcaaagacat attcgtagca taccagccgc cagcaacaaa cattgcagat
    49861 tcttgaaatg ctttctcagc atcttgtgca ccgtagaaat gtacaatatc tgagtcgatc
    49921 atttctttga ttaactttgc agcatgtttc gcttctttag tattcagacg gatatttcct
    49981 tcttcatcaa gcaaagattg attctgctgg gctagcatgt tgataaacaa ttcttggtcc
    50041 ccagattctg gcaaggcaat tccaaaacta ccagtttttt cttttaatgc ttttgccacc
    50101 tggatatatt cttcccaaga actgatttct tcaaaatcta cacctatttc agcaaatttt
    50161 tttggttgat aataacaaag tgctggggta aaatcactag cccaaggaat aatcgtttca
    50221 ttactcgttt gttgacgcaa aatattcaat ttttgatcat aaaactgatc gccatatttg
    50281 tcataaaatc catattcatc agcactataa aaagcatctt gaaatttttc cagcacacct
    50341 gctgctgaag tgtctgccat ggagataata tccggcatat cttcgtgtgt taccaatttt
    50401 ggggtgatca tttgtactgt tgctgttccc gaagctacat tcacaatttt taagtcaata
    50461 tttgctccag ttttgcggtt atagattttt gcagcatttt caacatagtt tgtcagtttg
    50521 ccgtttgcga catatacttc taatgtagtg gcttttttta tcattggatc tgcgatgttt
    50581 gctgttactt cttcgttagt ttttccacat gcacttaagc tgaataatag ggtaatgttt
    50641 agcaaagcta tcaccaattt gtatcttttt ttcatgtcta tcccttcttt cttttcaagt
    50701 tcttatataa aacg
//
LOCUS       DAZKBH010000009        46281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000009 DAZKBH010000000
VERSION     DAZKBH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.009"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACG8OO_000542"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACG8OO_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9044474.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACG8OO_000543"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACG8OO_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9044475.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACG8OO_000544"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACG8OO_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9044476.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ACG8OO_000545"
     CDS             1642..2274
                     /locus_tag="ACG8OO_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9044477.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ACG8OO_000546"
     CDS             complement(2276..3181)
                     /locus_tag="ACG8OO_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9044478.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ACG8OO_000547"
     CDS             complement(3266..3925)
                     /locus_tag="ACG8OO_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9044479.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ACG8OO_000548"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ACG8OO_000548"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFP9044480.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ACG8OO_000549"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ACG8OO_000549"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFP9044481.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ACG8OO_000550"
     CDS             6168..7253
                     /locus_tag="ACG8OO_000550"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFP9044482.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /gene="fni"
                     /locus_tag="ACG8OO_000551"
     CDS             7250..8314
                     /gene="fni"
                     /locus_tag="ACG8OO_000551"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9044483.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ACG8OO_000552"
     CDS             complement(8563..9366)
                     /locus_tag="ACG8OO_000552"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP9044484.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACG8OO_000553"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACG8OO_000553"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP9044485.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ACG8OO_000554"
     CDS             complement(10244..11251)
                     /locus_tag="ACG8OO_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFP9044486.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ACG8OO_000555"
     CDS             complement(11258..11902)
                     /locus_tag="ACG8OO_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9044487.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ACG8OO_000556"
     CDS             complement(12002..12760)
                     /locus_tag="ACG8OO_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP9044488.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACG8OO_000557"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACG8OO_000557"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP9044489.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACG8OO_000558"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACG8OO_000558"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP9044490.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACG8OO_000559"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACG8OO_000559"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP9044491.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACG8OO_000560"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACG8OO_000560"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP9044492.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACG8OO_000561"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACG8OO_000561"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP9044493.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ACG8OO_000562"
     CDS             18158..19027
                     /locus_tag="ACG8OO_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9044494.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ACG8OO_000563"
     CDS             complement(19153..20052)
                     /locus_tag="ACG8OO_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9044495.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ACG8OO_000564"
     CDS             complement(20217..21041)
                     /locus_tag="ACG8OO_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9044496.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ACG8OO_000565"
     CDS             complement(21052..22347)
                     /locus_tag="ACG8OO_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP9044497.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ACG8OO_000566"
     CDS             complement(22363..22842)
                     /locus_tag="ACG8OO_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP9044498.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ACG8OO_000567"
     CDS             complement(22848..23876)
                     /locus_tag="ACG8OO_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFP9044499.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ACG8OO_000568"
     CDS             complement(23893..24999)
                     /locus_tag="ACG8OO_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9044500.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACG8OO_000569"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACG8OO_000569"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9044501.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ACG8OO_000570"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ACG8OO_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9044502.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ACG8OO_000571"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ACG8OO_000571"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9044503.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="ACG8OO_000572"
     CDS             29970..30668
                     /locus_tag="ACG8OO_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9044504.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="ACG8OO_000573"
     CDS             complement(30836..33475)
                     /locus_tag="ACG8OO_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9044505.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ACG8OO_000574"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ACG8OO_000574"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9044506.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="ACG8OO_000575"
     CDS             35318..36493
                     /locus_tag="ACG8OO_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9044507.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="ACG8OO_000576"
     CDS             36497..37138
                     /locus_tag="ACG8OO_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9044508.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="ACG8OO_000577"
     CDS             37135..38052
                     /locus_tag="ACG8OO_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9044509.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="ACG8OO_000578"
     CDS             38049..38720
                     /locus_tag="ACG8OO_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9044510.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="ACG8OO_000579"
     CDS             complement(39261..39749)
                     /locus_tag="ACG8OO_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFP9044511.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="ACG8OO_000580"
     CDS             complement(39950..40216)
                     /locus_tag="ACG8OO_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9044512.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="ACG8OO_000581"
     CDS             complement(40216..40479)
                     /locus_tag="ACG8OO_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9044513.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            41000..41140
                     /locus_tag="ACG8OO_000582"
                     /pseudo
     CDS             41000..41140
                     /locus_tag="ACG8OO_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(41282..43105)
                     /locus_tag="ACG8OO_000583"
     CDS             complement(41282..43105)
                     /locus_tag="ACG8OO_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9044514.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..>46281)
                     /locus_tag="ACG8OO_000584"
     CDS             complement(43703..>46281)
                     /locus_tag="ACG8OO_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFP9044515.1"
                     /translation="KALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKN
                     KQLGWQFVDSKEQLEDGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKI
                     NAIAPKITDKGASSIQSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILS
                     TDENLDTIDGYTKQVLELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPE
                     LKDQAKIILDLQEKIPEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQD
                     ILPDVKNLENQASDLANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTV
                     RTAIEDGKLTDDEKKNISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPII
                     DQLNQLKPLLSNLQNRLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQI
                     SSTVTSLLDQVIDTIGTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAI
                     GQEIHDANTMLNGNMDTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEI
                     TGTLTTVNEKMPEVETAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKL
                     LKLDATKESDFFTQPVELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYY
                     LGKKDRNNFTKREQFVARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLI
                     ALAFMMIVYVLAALFDNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTH
                     AVNLLRESAGGIYWPNATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHI
                     FH"
BASE COUNT        13115 a        10014 c         8476 g        14676 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg a
//
LOCUS       DAZKBH010000010        41463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000010 DAZKBH010000000
VERSION     DAZKBH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.010"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..416)
                     /locus_tag="ACG8OO_000585"
     CDS             complement(<1..416)
                     /locus_tag="ACG8OO_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044516.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKN"
     gene            complement(427..1095)
                     /locus_tag="ACG8OO_000586"
     CDS             complement(427..1095)
                     /locus_tag="ACG8OO_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFP9044517.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1225..1809)
                     /locus_tag="ACG8OO_000587"
     CDS             complement(1225..1809)
                     /locus_tag="ACG8OO_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9044518.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2140..2373)
                     /locus_tag="ACG8OO_000588"
     CDS             complement(2140..2373)
                     /locus_tag="ACG8OO_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP9044519.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2388..3338)
                     /locus_tag="ACG8OO_000589"
     CDS             complement(2388..3338)
                     /locus_tag="ACG8OO_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFP9044520.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3335..4177)
                     /locus_tag="ACG8OO_000590"
     CDS             complement(3335..4177)
                     /locus_tag="ACG8OO_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFP9044521.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4179..4898)
                     /locus_tag="ACG8OO_000591"
     CDS             complement(4179..4898)
                     /locus_tag="ACG8OO_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9044522.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            5446..5748
                     /locus_tag="ACG8OO_000592"
     CDS             5446..5748
                     /locus_tag="ACG8OO_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9044523.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            5820..7046
                     /locus_tag="ACG8OO_000593"
     CDS             5820..7046
                     /locus_tag="ACG8OO_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9044524.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(7134..7526)
                     /gene="rpsI"
                     /locus_tag="ACG8OO_000594"
     CDS             complement(7134..7526)
                     /gene="rpsI"
                     /locus_tag="ACG8OO_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9044525.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(7542..7970)
                     /gene="rplM"
                     /locus_tag="ACG8OO_000595"
     CDS             complement(7542..7970)
                     /gene="rplM"
                     /locus_tag="ACG8OO_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9044526.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(8504..8971)
                     /locus_tag="ACG8OO_000596"
     CDS             complement(8504..8971)
                     /locus_tag="ACG8OO_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFP9044527.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9091..10629)
                     /locus_tag="ACG8OO_000597"
     CDS             complement(9091..10629)
                     /locus_tag="ACG8OO_000597"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9044528.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            10709..11395
                     /locus_tag="ACG8OO_000598"
     CDS             10709..11395
                     /locus_tag="ACG8OO_000598"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9044529.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(11596..12123)
                     /gene="msrA"
                     /locus_tag="ACG8OO_000599"
     CDS             complement(11596..12123)
                     /gene="msrA"
                     /locus_tag="ACG8OO_000599"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9044530.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12207..12884)
                     /gene="pgmB"
                     /locus_tag="ACG8OO_000600"
     CDS             complement(12207..12884)
                     /gene="pgmB"
                     /locus_tag="ACG8OO_000600"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9044531.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(12881..14011)
                     /locus_tag="ACG8OO_000601"
     CDS             complement(12881..14011)
                     /locus_tag="ACG8OO_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9044532.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14011..16194)
                     /locus_tag="ACG8OO_000602"
     CDS             complement(14011..16194)
                     /locus_tag="ACG8OO_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFP9044533.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(16191..17216)
                     /locus_tag="ACG8OO_000603"
     CDS             complement(16191..17216)
                     /locus_tag="ACG8OO_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9044534.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(17261..18088)
                     /locus_tag="ACG8OO_000604"
     CDS             complement(17261..18088)
                     /locus_tag="ACG8OO_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9044535.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18085..19152)
                     /locus_tag="ACG8OO_000605"
     CDS             complement(18085..19152)
                     /locus_tag="ACG8OO_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9044536.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(19178..20014)
                     /locus_tag="ACG8OO_000606"
     CDS             complement(19178..20014)
                     /locus_tag="ACG8OO_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9044537.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20011..20880)
                     /locus_tag="ACG8OO_000607"
     CDS             complement(20011..20880)
                     /locus_tag="ACG8OO_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9044538.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(20950..22233)
                     /locus_tag="ACG8OO_000608"
     CDS             complement(20950..22233)
                     /locus_tag="ACG8OO_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9044539.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(22247..23932)
                     /locus_tag="ACG8OO_000609"
     CDS             complement(22247..23932)
                     /locus_tag="ACG8OO_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP9044540.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            24207..25232
                     /locus_tag="ACG8OO_000610"
     CDS             24207..25232
                     /locus_tag="ACG8OO_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9044541.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(25827..29480)
                     /gene="rpoC"
                     /locus_tag="ACG8OO_000611"
     CDS             complement(25827..29480)
                     /gene="rpoC"
                     /locus_tag="ACG8OO_000611"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9044542.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(29559..33176)
                     /gene="rpoB"
                     /locus_tag="ACG8OO_000612"
     CDS             complement(29559..33176)
                     /gene="rpoB"
                     /locus_tag="ACG8OO_000612"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9044543.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            33904..34872
                     /locus_tag="ACG8OO_000613"
     CDS             33904..34872
                     /locus_tag="ACG8OO_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9044544.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            34890..35795
                     /locus_tag="ACG8OO_000614"
     CDS             34890..35795
                     /locus_tag="ACG8OO_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9044545.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            35792..36598
                     /locus_tag="ACG8OO_000615"
     CDS             35792..36598
                     /locus_tag="ACG8OO_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044546.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            36755..37723
                     /locus_tag="ACG8OO_000616"
     CDS             36755..37723
                     /locus_tag="ACG8OO_000616"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFP9044547.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(38040..38564)
                     /locus_tag="ACG8OO_000617"
     CDS             complement(38040..38564)
                     /locus_tag="ACG8OO_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFP9044548.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            38851..40548
                     /gene="scm"
                     /locus_tag="ACG8OO_000618"
     CDS             38851..40548
                     /gene="scm"
                     /locus_tag="ACG8OO_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP9044549.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(40703..>41463)
                     /locus_tag="ACG8OO_000619"
     CDS             complement(40703..>41463)
                     /locus_tag="ACG8OO_000619"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9044550.1"
                     /translation="IEMLHTATLIHDDIVDEADLRRGQPNIRSQFGNVVSVYAGDYLF
                     VCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYNYELSVKEYLENISGKTAE
                     LFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDILDYTKESQEIGKPVLEDM
                     RQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRKIVEHTGGVEEARKLAASY
                     TEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
BASE COUNT        12221 a         8617 c         7409 g        13216 t
ORIGIN      
        1 atgtttttat ctaatatatt tgaaagatct aataattcag ctaattcatt gattctttta
       61 tccatatgct cttttgtaaa tccaaagttt tccatcgaaa atactaactc actcgttgta
      121 tttgttgtaa agaattgtcc tctaggatct tgacttacgt agccaatatt ttttgatata
      181 ctttcttgtg ttaaggattg tagcgagtta ttttcaaata aaatttcccc aaccaatttt
      241 ccgtcataaa gtcttggaat aagtccattc attaacttta atagtgtgga tttcccactc
      301 ccagaagaac cagtaatcaa tgtaagactg ccttttttta ttgaaaaatt aaggtcaaca
      361 agcgcttgtt ccttaacatc cttatatgaa aaagaaacat tgttaaattc aaacaattct
      421 ttcacctcaa atcaaaatac ttaagaaaaa aatagtaacg gaaaacagga tgatatagtc
      481 taataagctc atgtttgttt tatagtaaga acttcttttc acaggagatt ctatcccctt
      541 tgttattgca ttagcggtca tttcttcagc aatcatagtt gcacgtataa gaacgggaac
      601 taacagaaat tccaaatatt gcagaggctg aaaaaaagac aagcgaatgt ttctcatttt
      661 taaagattct ttaatcattc ttatttcaga acgataagtc ggaaagaatc tgatcactac
      721 tgtaatactt aacattattt ttttcggtaa atgtattttt tctaaagagg ataataattc
      781 attgctagta tagacactaa ccaacaacca tgagatcatt acgaccggca acattcgaaa
      841 tacaatatat aaggatgtcg caaaaaatat agtaaaaaac tctatcttga tagcatcaat
      901 aattttcatt actaaaaaca gaactgaata aatcaagatg aaataaatac atgtctttat
      961 tttacgaaac atcaatacta aaactgtagc aataatgaaa caccccactt gaaatatatt
     1021 tccctttcct aaaaaaataa tgaccggaaa aaaaatattt aaagtaatca aggttaacgg
     1081 attgattttt gacactcttt cacctccaaa aaataagatc gcactgttat cttaagctga
     1141 taaaaatgca ccgttattca gataacagtg cgatcttatc aaaaagcata actgagaaca
     1201 attctcaatg caagcttttt ttaattaact cagtaattca atccacccag gaacagaaaa
     1261 ttgatacaat cttctggtta atagcatagt cttttctttt gaatagctat gcttcaatat
     1321 atcatagaat gtagcgtaga atgagtaaat gtagaagtgg atttcttctt cattaaagac
     1381 taaattttga ttgatcattc cgatagtctt taacttatct agacaacaga ctgtcttttt
     1441 cgtcacaaat gatgtaattt tttcaacaat atcttgatat ttggttccag ctgattggaa
     1501 taaaatcaaa tcgaatatac gtttattctc aaaaatataa ttaataaatg gatctaattc
     1561 attgaagttc tctctccaac ttttctctat atctatattt tctaagttta agaggtattt
     1621 ggtaaactct tcattataca tattaataag cgcattaata tgcggctgaa taatttcttc
     1681 aaataacgct tccttagaag taaaatgttt ataaaaagca cccgtagtta cgcctgcggt
     1741 caggcacagt tgccttatct tagtttgctg aaatccttct ttttcaaaat ggcttagtcc
     1801 actttctatt aacttttggt gagttataat ataatctctg gtttctatgg gtacttcctc
     1861 ctaataagat gaattaactt tattatctta cacaggactt gaagtttcaa tcgttaacgt
     1921 ttattactga actcattcct tagagctaaa ctaatgagaa agagcagaat aactgataca
     1981 aaaagcaatc ctacaagaat taatagactc atctaaatca ttcacctttt ctaaaataat
     2041 ccgagaaaga tttaaaaaaa ttatatccag ctattaggat aagtcctatc ataatcactc
     2101 caatagccat tttcttatcc tccctaaaaa taaagtcttt tacttaaaac cttttttgta
     2161 atcgtcaata aattttttcc ctttatcagt taattgaaat cctatatcta aatagtcctc
     2221 atatctaagt ttagccagca tctcacttac agatgctgga ctaacactta gaacttttgc
     2281 taaatcatta ttttttaatt tcttttttct ttttgataat tcctgaattg ctagtaaata
     2341 aattagtata gtagtcgttt taatttgtac catacctacc ctccaaatta tttcgaaagg
     2401 ccttcagcaa ttttatccag gttccatttc atcatcgcat agtaactatc accatcttgt
     2461 cctttttctg caattgaatc agtaaaaatc gttgaataga taggaatatt ggtatctttt
     2521 gatactgttt tcattggtct atcatccaca ctgctttcta cgaataagga gggaactttt
     2581 gttgtgcgta atttttcaac taagtgtttt atttgatctg gtgttccttc ttcttcagtg
     2641 ttgatttccc aaatgtaagc agaaggcaca ttatacgctt tcgagaaata tttaaagcat
     2701 ccttcacttg taacaatcat ttttttatca ttcggaattg aagcaaattt agatttagct
     2761 tctttgtcta gagaatccaa tttttcaata tacttatcta gattttcttt atagaatttt
     2821 ttattatcag gatctttttc cgctaactgt ttttcaatat ttttagcgta aataataccg
     2881 ttttctaaat ttaaccaagc atgaggatct tccttccctt tctcactctg accctctaag
     2941 taaataacat ctatgccatc acttgctgca aaatagtctt tgttttcctc tttgttcgca
     3001 tttttaacta attttgtaaa ccaagcattt cctccagttt ccaagttaac accgttataa
     3061 aaaatcaaat ctgcttttga agttttttga acatcttcag gcaaaggttc atattcgtgg
     3121 ggatctttcc caataggtac gatactgtgt aaatcaattt tatcttttgc tatattttcg
     3181 gtaatatctg ctaaaatcga attagtaact actactttta atttttcgtt cgaagccact
     3241 ttatctttgt ctttactatt tattgaacat gccccgaata taaaaagagc aatcaaaatc
     3301 cccagacttc ccagtactgc tttccatttt ctcatcataa cgatctctcc ttctttctta
     3361 aaaatttttg ttttggtgag attagaaaac taattacaaa taagaatgcc gctgttaaaa
     3421 caatacttga tcctgctgct atgttaaaac tataaccaat aaacaatcca agcaatgatg
     3481 aacttgctcc gactgttgca gaaataaata tcatagtttt caagctattt gcatataaat
     3541 atgctgtagc tgctggtgta attaacattg caacaattaa tatagttccc acactttgca
     3601 tagctgttac tgctactaaa gttaatagaa ccatcaatag ataatgataa aaactgacat
     3661 tcatccctat tgcttttgcc attaatggat caaaagaagt gattaataat tctttaaaaa
     3721 atagaactac tataaccaac actaaaaccg aaacgccaat tgtaatccac ttatcaatat
     3781 cttgtacagc taaaatattt ccaaataaaa tatgaaataa atccgttgag ctatttgcaa
     3841 ctcctataag gataactcct agtgctaaaa aagaactgaa agtaatacca attgctgtat
     3901 ctccttttat tacactattg ctttttataa aagtaataat gatagaagct aatagaccaa
     3961 atacaatcgc accaataaaa aaattaatac ctaaaatata agaaattgct actcctggca
     4021 aaacagcatg agaaatagca tcacccataa gagacattcc tcttaaaata ataaaacaac
     4081 ctactgttcc agaaacaatc cctataacta cagatgtaat tagagcattc tgtaaaaagt
     4141 gaaagtcata cagaccatta ataaaatttt gaagcatatt attccccctc tccaataaat
     4201 atagtatctc cataggcttt ttttaaattt tcttcattaa atacagattc tacacttccg
     4261 tgagcaatta gatttcgatt caaaagaata atattatcaa aatacttctt taccttgctt
     4321 aaatcatgat gcacaattag cactgttttt ccctgatgtt tcaatttttt taacgtatcc
     4381 ataattattc gttcactaac taagtctatc cctacaaatg gttcatctaa aaatatgaaa
     4441 tccgcatttt gagcaagaca tcttgctaaa agtacacgtt gaaattgtcc tccagataat
     4501 tctcctattt gatgattaga atattttccc atatcgacct tttccaaagc ctttgaaact
     4561 ctttgccatt ctgcattttt aattctttga aaaactttca ttccggcata tgttcccata
     4621 gatacacatt cttttacttt tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca
     4681 tatgcaatct tcaccagttt tttattgata tcttcattat taatggagac ctctccctga
     4741 tgagggataa tatttaacat tgctttaagc aatgtggatt ttccagcacc atttggacca
     4801 ataatacctg ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa
     4861 tcgttataac aaacattaag ctttttcact tctaacataa ctttctcctt ctaaatgata
     4921 atgattatca cgatcatatg gtatacaaaa attatatttt agtcaaccct aaattttata
     4981 gtttagattt ttttctgttg ctgaagatta gaatgacaaa actatttgaa actacgcgta
     5041 tagtaagtag ctttcaaaat atataaaaaa ggagcgttct caaatgagaa catgctccta
     5101 aaactacttt attggcgtat ggaaaaatta tacttgaagt ggatagcggg ttgaataagt
     5161 tgataagcat caaaacattt ataaaaatta ttcaagacta tgtctgttcc atatacaaat
     5221 atgaaaatca gtaaactcca tttaactttt ttcgtagtgt gtctttttat acatgtttat
     5281 cgttattaat agtgaatagg aaaaaaacta tttttgatca ttgacaatta aatacaattt
     5341 ttgaataacg atccctgcta ttcgagctag aaactcaagt acgccatgac ctgattagcg
     5401 acaaatactt agagatttct ttttgttctg ggaaaacaaa atgccatgac aatagcagca
     5461 aataatgata cttccgtaag gctcgccgta aactggggag aggtgaaatt cctatgtgct
     5521 tacccaaagc aattcaaaaa tttcataact actattttag gagatgattt tatagaatcc
     5581 agagtctacc tgaataaaaa acaaacctgt gcatttctag gaatcgataa taatactcta
     5641 gataagtgga ttcgtatata taatttccca gtaataagag ttggtggtgt agttcgcttt
     5701 gacgtagaag atattaaaga ctttatgaat acatttaaaa taacttgaaa ttttcttctc
     5761 gccgcatata ctaaaattag tgaaatatac tgccttactt tttttaagga gtgaggattt
     5821 tgcctacaaa gctttcaaat gggaaatata aaactaatct gagatatcca aaaaggttta
     5881 aagaaataac aggaattgca tcagaaaagt atcaaaaaac atttccaaat aggcaattgg
     5941 cgattaaagc cgaaaatgat atgaagaaga aaatagaaaa agttttgaga gaggagaatg
     6001 caaactcatt agaattaaaa ggaaaaataa cgtttaaaaa attttacgaa tctaaatggc
     6061 ttcctagata tgaattaggt caaacaatta gatcaaacag gcctcctagt gatataacca
     6121 ttagtaatac aaaagatata tttcggttgc atattttacc tatgtttggt gaatacgcaa
     6181 tgaattatct aaatatcaat actgagataa tatcagatga attaaccaaa aaatcgaaag
     6241 aatatgcaaa tataaaaatc ataaaaggat atgttcgttc catgttcgat attgcagaaa
     6301 tactaaatta tatcgaattt aatcgaacta ctaaaattat tcaaagtatc actgcaccta
     6361 aaaaaaatgc tttagaagaa aaaagaatac aagaaggtaa gcaagcacta tcatcaaaag
     6421 aacttactaa ttggatagaa gcagtaaatg atgattttaa taatcattta cttacttttc
     6481 atgattacac actttttatg attaccctgt atcttggtga tagaaaaagt gagacttacg
     6541 ctttgcaatg gaaatatatt gattttgaaa aacaaacggt ccgtcttaaa catactttag
     6601 ataaatatca aagaaaaaag tttacaaaag gtcgaaaaga taccgtcatt caagtaccag
     6661 aagttgtaat gacattgcta agtgaatgga aatcggtaca agctgatcaa ttgttgaaat
     6721 taaaaattaa gcaaacgtta gatcagtatc tctttactta tactaaacca tctggagaag
     6781 taaattgtcc tgttcatgcc gattacctaa actatcgaat caattcaatt aaaagaaggc
     6841 atcctgactt agttcattta tctccacaca aactccgtca tacttatgca actatagcta
     6901 gacaaggtgg agcagatatg aatcaaattt caaatgcttt aacacactca gatatttcta
     6961 caacaaagat atatgtgaac acaccagata tagttgataa ggctgttttt gaagcttttc
     7021 agagaggatt gaataagtgt gattgatatg tgttcttaaa tgtgttctta aaaacaaaag
     7081 aagcactttc caaataattg aaaagtgcca gaaacgttga tatataaagg atattaacgt
     7141 tttgagaatt gtgaagcttt acgtgctttc ttaagacctg gttttttacg ttcgaccata
     7201 cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta aacggaagtc aggatcaact
     7261 tctagcaatg cacgagcgat accatgacgg atagctcctg attgtccagc gtatcctccg
     7321 ccgtttacgt taacaaatac gtcatatgcg ccttttgttt cagtaacagc gaaaggttga
     7381 ttgattactt cacgcaagtc agcatgtgga atgtattctt caacatcttt tttatttaca
     7441 gtaattttgc cagttcctgg tactagacgt acgcgagcaa ctgcgttttt acgacggcct
     7501 gtgccgatat attgaacttg tgccaatgaa attccctcct attagattaa gtttgtgatg
     7561 tctaaaactt ctggttgttg tgctgcatgt ggatgttctg ctccaccgta tacgttcaat
     7621 tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca taccttttac agaagtttcg
     7681 atcaaacgac gagaattttt agcacgcaat tcgccagcag tgattgattt caatcctcct
     7741 gggtagttgc tgtgacggta ataaattttg tcagtcgctt ttttacctgt taatttcact
     7801 ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt
     7861 ttatttttac cacgtagtac agatgcaaca actgttgaaa gacgtcccaa tggaacatca
     7921 gttgcgtcta ctacatacca tttacgttct acttcgcctg gtttggccat atatgttgta
     7981 cgcacgttat tttcctccaa tttaagttct gatgtttgac atacacttcg agtttccggg
     8041 gctctttgtg gggcaaacaa taccatttaa catattaccc gctaatctag tcaatgtcaa
     8101 cgtttttctg cttgattgaa aaatttatat gaaactttac ttcttttctt tcttttatat
     8161 aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca
     8221 caacccctta ttttgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
     8281 atccataaaa cataagaagc tgttttttaa aattgtttct tgtgactcgg agctgaacgg
     8341 caggcttaca acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa
     8401 aaacggcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg
     8461 ctgatttcac aacctctttt tcggtcaagt tcaacaacag tttttacgcg tcgatctctg
     8521 gcgctttgcc caaatgagct tggatcatcc agatacgttt ttctgtggct gttttgaatt
     8581 cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg attccttttt
     8641 ggtaaagatc tgctagataa cgatagcctg caacaagtgt ttccaaacgt tctggaatag
     8701 tacgatccca gcggccaatt tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg
     8761 tagaataagg tgaaccgtct aatgtgatca agcgttcgga tacttcatct aactgagcgt
     8821 tcaaatcatc catgaactca tccatcattg ggtgcaatgt aaggaaacct ggtcctctca
     8881 tgtaccaatg tgtttggtga acaacaacag acatttggct tagatctgca actaattgat
     8941 ttaatacttc tttggtttgt tggaatttca tctttcttgc ctcctaacat tttcttacac
     9001 ttaaaataat agtagacttt caatgaaaag tacaacaaaa tcatttacct gagaacgatt
     9061 ttcagttcgc ttttttatcc gtaaagccaa tcatttttca gaatcagcta attcttctcg
     9121 cagttgagca aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg
     9181 atagattttc tgatattttt tatgatcagt ttcttgtact ccaacttgct ctttcaattc
     9241 tatcaattcc tgtgcttcac tgatactttg gatctttccg atgcttctcc agcctaaaat
     9301 cacagctcct aatgcggatg cttctgtgac agtagggaaa acaagcgggt aacccagtac
     9361 atcagccatc atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag cacggatctc
     9421 tgtgagctga ttttcctggc aaactccttt taaaatccga taaaggttga gacagatccc
     9481 ttctagaatc gccttcatca tttctttttc tgtatgttgc cgctgtaaac cgataaaact
     9541 gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat
     9601 cagaccattg gcacccgctg gcacaaaagc gatagccgaa agcagtgcat catatggatt
     9661 ttctcctttt tctttagcag attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc
     9721 aaagatgcca gcaccattgc tagtcgctcc tcccactacc caatgtttct gatccagaac
     9781 ataacaaaac gtctccattt ctggatggaa ctgaggctga gtagtaataa aacgaagtgc
     9841 tccgctcgtt cctacggtca aagcggcgat cgtatcacca aaagcaccta accctagatt
     9901 agaaagcggc ccgtctgccc ctcccaaaac aaatgatata tcatttggca aacctagttc
     9961 tgctgctgtt tttgatggta gacctgttac ttgataggtg gaaggaaaga cttgcggcaa
    10021 ttgctctatt cgaatgttca agtagtcaag cacttcttct gcccattgga attcatggat
    10081 atcaaaatat ccagttcctg aagctgttcc gtagtcgctg atcagttcgc caaacaatct
    10141 ataaaagata tagtctttga taccgacaaa ataagcagtt tttttgaata attcaggttg
    10201 gctgttttgc agccacttga ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt
    10261 ctgccgataa aattctttgc caagcacggt atatttcagt ttttctgctt cgtccgctgc
    10321 tcggttatct gcccaagtga tcattcgaga taacggtgtt ttctcttcat ccaacaatat
    10381 caggccatgc atcgcagagg agaaggagac aaattgaatg attgtgtctg aaggcaactg
    10441 tttaattact tcttttataa cggtgcagac accttgaaag atcaattcct gatcttgttc
    10501 tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg
    10561 ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta atccgattga
    10621 aacgttcatt ttttcacctc aaattttatg tatgtacaaa aatttagttt aagcttgcgt
    10681 atcttggttt tgaaaagggg gagatggcgt gttactttat tttatcattg gttgctgtat
    10741 cggatcattt ttatgtttaa ccgcccagcg gattccgtta ggtcactcta ttatttatcc
    10801 tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga ttccgattct
    10861 ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc ccttctacta
    10921 tttgttggca gaagccttat gtggtggtct ttttgcctgg ttctttactt tttcttccgc
    10981 aagaaatttt tcgacgttca tatggatgct ttcggctttg ctattttcgt tgatggattt
    11041 attttacttc atggtccatt ctcacacgct attctgttct tgggcaattt tatggatttt
    11101 ttggttgcag gccggcgtct tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg
    11161 tgtgtgcctt cgctatggtc aatcctatct cggagctggc gatactctgt tgatgctttc
    11221 ttggagtggt ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt
    11281 tggaatatcc gttttttctg tttactttat ctgtcataaa aaaaagttgg aaaagctgcc
    11341 tttcattcct tttttgagca gtgcgttgct gattgtcctt catcttaggt attgaaaaaa
    11401 ggttgtgaca aacgacttgt cacaacctct gatatatagt gttctcttac caaatccttg
    11461 tgacttggag ctaggcgaca ggttcacaac ctctccacta aatggtgttc aatcagctga
    11521 ccctcagtat tagctcaaaa tgctggtttt acaacctctg atatacggtg ttccataagt
    11581 agctttttcg gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc
    11641 agaaccttct cgataaagat tgtaatggag cttgtttttc ttgtaataat cctgatggta
    11701 atcttctgca ggataaaatt cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa
    11761 tcgcccgctg tcttgcaatg cttgttttga acgctctgca atatttcgtt cttcttcatc
    11821 attatagtag atcactggac gataagaatc accgcgatca gcgaactgtc ctaatgcgtc
    11881 agtcggatcg gtttgctgcc aatagatttc caccaactga tcataagaaa taacttctgg
    11941 atcatattcg atctctactg cttctgtatg ccctgttgtg cctgttgtta cttgttcata
    12001 agttggattt ggtacatgcc cgccagtata accggatgtc acagaaatga tccctggctg
    12061 ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt
    12121 catataaaat cccccttttt tctcatcata ccttgatttc caaaaggagg ataacgtttt
    12181 gcttgtgaca gaaaaaacgg aaactattat atacgtgata cggcttctga taaatgccaa
    12241 aacgaacgta cttgatcaaa aacggttaaa tcaggaatca catattcagc agaaggaaga
    12301 tttatgtttt cacctattcc caatacatgc atgttaccag ccaaagctgc ttgacaacca
    12361 gcttctgaat cttcaatgac caagcatgca tttggcggta cattcaattg ctgtgctcct
    12421 ttgagaaaaa cttctggatc aggttttgct tttgatactt gcgtgccgtc aataaggaca
    12481 tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt gcttgctgat
    12541 cccaatccga tcttgatgtt tgtttttttc aagtactcaa gaacagctat tgcaccaggc
    12601 aaaacggatg tttcatccat tttttgaatc gcttgaagat atagattgtt tttttgctca
    12661 gcaaaggctg atttctcttt ttctgagaat ttctgttcca tttttccaaa tgacagcaat
    12721 cgctctagtg actcggttct ggatattcct ttcagttgct cgttttcttt ctcagtgaat
    12781 ggaatggaca gctggttagc taaaaccagc cacgcttcgt aatgataacg agctgtgtct
    12841 acaagaacgc catctaaatc gaatagtgct gctttgattt tcatcgatta cctccttgaa
    12901 aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg
    12961 cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa
    13021 tgccaacatc tgctgctttt tcgcttcctt taccaacact tccgcaaata gctatcacag
    13081 gaacgccctc tggtactctt ctagcaatcc caactggcgc ttttcctttt agactttgtc
    13141 catctagacg tccttcgcct acgattacga ggtctgcttg ctgtacccgt ttatcgaact
    13201 ccagtaaatc caatacgtag tcgatgccag agactatttt ccctgaagca aatgctaaca
    13261 gtcctgcacc cattccacca ccagcactac tgcccgatgc ctcgattact tgaggggcaa
    13321 atctcatata aaaatcatgc atggcttggt cagccactgc taattcattt tctttcaagc
    13381 ctttttgtgg tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga ttagtaacat
    13441 cagtaatgac agtgattttg gctttaatca gctcaatagg cacgttcgtt ttatcaactg
    13501 tagcaatttt acctagattt tctcccagtg cttctacttt ttgattatct tgatcgtaaa
    13561 aagcataacc tagaccttct gccatgccga tgccgccatc gttggtacta cttccgccaa
    13621 cgccgatgat gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt tctcctactc
    13681 ctcgcgtgga taacctaagt ggctgttgtt ggtttagtgg gacatctttc aatccgacga
    13741 tttctgccat ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt tctttgtttt
    13801 tgccaaatgc attagtaacg actgctgttt gccgcttcaa ccttaaactg tctgctaatg
    13861 atgaaaccgt tccttctcct ccatcgccta taggtagcag atcatattca gcctgaacaa
    13921 aaacttcttg aaaacctgat tgaatggctt gtgctgcctc taaggctgtc agactttctt
    13981 tgaaagaatc tggcgcaatc agaatacgca ttattttccc ctccgttcca agatcagttc
    14041 tttttccagc tggtattggt ctccattgac aaaaataggt aatgtttctt tcgtctcttt
    14101 ttttattact atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt tccaaataaa
    14161 aggaaaagaa attttttccc atgctgaagg aagtcttgga gaaatagaaa gagttccatt
    14221 cgttactgta atacctgaaa aaccaaatat aactgctagc catagtgctc ctaatgaagc
    14281 agcatgaatt ccgtcatctg atgaatgcgg atctggtcct aaatcaatca ggcacgcttc
    14341 ttggaataaa tgatacgccc attccatttc ttggatacgg caagcctcta tggcgtgaat
    14401 cgctttactc aacgatgagt cgtggattgt tttgttttca tagtaatgca aattcttaga
    14461 aacgattggt tgagaaaaat actcaggtaa taagtataac agcatgacaa gatctgcttg
    14521 cttgctgact tggagttcat ttacttcgct acgggcataa tcaagtaaaa tagactggct
    14581 gccaggacgt tctttgtaag tggtcaaatc aataactggc ttactgagat acgtgtcatc
    14641 ttgcggaatc agctgttctt tgttaggcac tggcaaatat aacttatcta aaaaattttg
    14701 acattctttc aaaaaaagcg gatcgccagc ttgatattta acagctaagg ccacattata
    14761 atgcgccaga tagttcgtat atgcattatt gtcgatatat tctgtgtatt catctggacc
    14821 aatgactctg tgtatctcta aatgatcatc gacttttaca gctctgctca gccaaaaagc
    14881 tgctgtttca tgaatgagcg tttttccctt tgtttctaaa aacgattgat cctctgttgc
    14941 ttggacatac tcttgaacgg cataagcaag gtctgcactg atatgatgtt ctgcggcagc
    15001 tgaagcaact tcttgtcttg ttccggtttt gatattcaat gcagcaaatt tgggtgtctc
    15061 ttcctgacca gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt gataatctct
    15121 tgctttttgc cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat tctttgctac
    15181 ttcaggaaac tgatataaaa agaaaggcag gataaaaatt tccgtatccc agaaaacatg
    15241 ccctttataa ccttcgccag ttagtccctt cgcccctaca ctaacacttg gatcattgcg
    15301 gggagtcata atctgtaaat ggtaagcagc gaagtccatc gctaactgat cgaaagaaga
    15361 atcgccttgg atcgttatcc gatgctgatg ccaaaaatct gtccaataag acatcgactg
    15421 ctctaaaatt tctgtataac taacgctttt ctgggcttgg gcaatacttt tcttaaacgg
    15481 gtctgtttgc tgaagagagg tagagagcac gatgatcttc tccagcgtaa aggacgtatt
    15541 ttttctgatc ttttctgaaa aggaagcggt gatccggcga ttttttgctt ggaatacacc
    15601 tgctttattg aatttagctg caatcccaat atcaatttta ctttctgttg tctggtagga
    15661 tgcactgatc aaatcgtcac caaatactcg cagttctttt tcaaccaact gctgtgtacc
    15721 aaaattggtt tgctgtccat cgatacccgt tttcacatct acttggcagt cttcggtcaa
    15781 cggcttgatg accactttca gggctgcctg atggagatta ttttgatcag caacacgaca
    15841 agtaatgact tccacttttt ttccttgagc agtttcaatg agcgcatagc gtttaagctc
    15901 tcccatttct aaatctagtg cccgctcgta tgcaagaact ttcatcgtct caagagaaaa
    15961 atgttcaccg tcgattttta tttcccatct gatgacatct gggagattca ctaactcggc
    16021 tgattctgct cctaaaggtc ggttatatac accagctgca aacattcccc ttacttgatt
    16081 ccgatagtct tcctcctggc ttcctctgat accaaaaaat ccatttccaa tggtcatctg
    16141 actcgcatac ttattgatat actctaaatc aaactgtttt tcttctactt tcataaggta
    16201 ataacttctc ctgtttctgc cgaacgatat aaattttcca ccagattctg gatgacatat
    16261 ccttgccagc catctgcaat catctgagtc tggtcaccga gcgcccgatt cacaaaatga
    16321 gccatactta gttcatgtgg gtcattaact ggcgtggtct tttgtaagga aaccagcttt
    16381 ccatctacat cttcgaatag ttcgcctgga tacagattag cacctgcctg atcgccacaa
    16441 aaagccacat tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt cagttggatg
    16501 atcttgccgt tttctaattc cagtgtgcca aaaattgcat cttctactgt gaatttgctt
    16561 ggatcccatt tcccgaattt ccctgaattt tttttcgtac caattttttg gaagctgtac
    16621 gcccgaacgc tttttacttt aggaaatcca gtaatataca tcgctgtatc aagcatgtga
    16681 atgccataat cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa cgttccccag
    16741 cctggaacac cagaccgacg aagcgcattc gcttctacgt aataaatatc cccgagaaca
    16801 tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa tccataagcc
    16861 aaaatctgcc cgtttttatc ggctgtctct gccatctttc ttgcttgatc agctgtcata
    16921 gctggtggtt tctcgcacat cactgaagca ttagcttcga gggcagccaa tacatgttcg
    16981 aagtgaaagc gattgggtgt acagacactc acgatatcgg gagaaatctc attcagcatt
    17041 tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc ttctgctcgc
    17101 tggctgactg gatcaaccac agcggccaat tctaagacat ctgatctttt ttgataataa
    17161 gcagcatgta ctttttctgc tacttgtcca gcgcctatga ttgctacttt cttcatttat
    17221 ttctctctcc tttgcttatt tacttattgt tattctttct ctattctttc tctaaatcat
    17281 ttaaatgacg cgctgtttcc agacagtttt tcaagtattg caatgattcc tgataaattt
    17341 tcacttcatt ttctcctcgc aaacgacatt catataccat ataaccttca tactcatttt
    17401 gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt tggtagcgat
    17461 gattatctgc taaatggata tgacccacat actctttttg cgtagtcaat gcttttgaaa
    17521 tatcatcttc ttcaatattc atatgataaa aatcagcgat gatctgcaca tgtttcaatt
    17581 gattagagtc aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg tcttggtatc
    17641 ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc ggctacttta tctaaataac
    17701 gaagtgattc gctgactgct ttatagtctc cttctttgct tcggggtgaa accatcggcg
    17761 gtaatcgata agtgaacatt ccccaagctg ctgggataac gattcctttg cctccaacct
    17821 cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc attaagacgc cgttcttcga
    17881 taaagtcacc gatccaccca tcgtagccgt tacaagctgt cactactggg atccctgttt
    17941 ctttgattgc ctgtttgact gtgtcgatat tttctattaa caacttaccg tcgatttcgt
    18001 attcttcaaa tccacagtct ttgataaatc taaatttttc taaaatgtct cttgggaaaa
    18061 atgcttgatc ttgcgtaccg aatttcattg ttctcctcct cggacttgaa cgcccatttt
    18121 gatactttct tctgggtgct ggtctacata acgcatgtaa gcttctgcgc tttctgcaaa
    18181 tggcacaact ggatcaatga tttcttcaca atccaagtaa ccgttcatca ataattccca
    18241 acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa tctgggtttg gttcactaga
    18301 tgctcgagca aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa gattaaaccc
    18361 agctggaaaa ggtttagcaa aggcaacata agaaatgatc ccgccatagc cgatccctat
    18421 taaagaagct tgtaatgcgg cagcactgcc actcgtttca ataactgaat ctactccacg
    18481 tttatctgtc aattccttta gacgaaaacc gacatctgct ttcataggat ctagcgtgta
    18541 atcggcaccg tgtttttctg ctaattctct acgctgcgct atgggatcga tcccaacgat
    18601 ggtagtagca cctgcttttt tagctaattg aatcgcaatt tgcccgatag cgcctaaacc
    18661 gaagattgct acagcatcac ctgctcgaac atgtgcttcc cggataccac ctaaagcgaa
    18721 ttgagcagga tcgtaacata ctgcattttt ccattgatcc aaccgatcaa gtttacgtag
    18781 tttataatta tcaacaccgt ttgtaatcag cgtttcacga atgggtccat aagtgcagac
    18841 taactctcct ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg taccgacaat
    18901 catatttcct aattgaaatt cgccaaatac cacaccgcgt tggcttcctt cttctcttgg
    18961 caagaatagg cgccattctt ctgaaaattc ttcatcgata aatggactta atcctctaaa
    19021 gtcgacaatt tctgttcctg gttttggagt agcgaaaatt actcggatac gtacttcgtt
    19081 cttttcgatc tcacggtctt cgtaatcttt gaatccggct attcttggtt ctaatgcaac
    19141 taattttttc atcttttctt tcctctttct tttcttttta tccttttacg ccgccttcga
    19201 ctaaatcacc tttaataaat cgttcagata acgcatacat aatgatgact ggtaaagcgg
    19261 tcaccaatga agcggccatc attcgtcccc aaatgtagtc tggtgtactg aataatgtgt
    19321 tcaaaccgat tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa gcaaataaat
    19381 aatcattcca cgaaaccata aagcaataaa cgaagacaga aatgattcca gaaatagcca
    19441 atggtataat gatccgaata atgatctgga tccggctaag accatcgatc attgctgctt
    19501 cttcaatatc agtcggaatc gtgtcaaaat aactttttag catgaatatc gcagtaggaa
    19561 gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt atcatacaaa cctagaccgg
    19621 aaatgatttt aaataatgga acaacgatca aaataccgga aaacatataa acggtgtaaa
    19681 agctagcatt gattttcgag cgagctttga agcgtagttt tgacaaagcg tatgccccgc
    19741 tgatcccaat cactactgca gttacggagg ctatcaagga gatcactaaa ctatttttga
    19801 aatacatgat gaatggaaaa atctcagcat tgaaaatatc ctgataatgt tcaaaagtcc
    19861 attctttcgg aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc ttgaatgatg
    19921 tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa atcgtcgcgt
    19981 aaaataagat tttcctgctc tttttatttt tcatttcaag tccaccttct ttcgtgagag
    20041 caagacgacc gctgcaataa tgatgaacaa tagaatcgat atagctgctg cttttccaag
    20101 atcattgaac gcaaaggctg tctgatacaa ataaacgcct aagatattga ctttattcgt
    20161 cagtagatag acatctgtaa acatatagaa catccagata gatcgtaaaa caatcacagt
    20221 tgctagtact ggtttgattg ccggcagtgt cacgatcttg aacttctgcc aagcatttgc
    20281 accgtccatt tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa tagccaaaaa
    20341 tgaaataaac gcatacggat agtatctcca gatagcaaac aaaacgacca taaagaagct
    20401 agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc tatctactaa
    20461 cagatcattg acgattccat aactattatt gaacatatat ttccaagcaa aaatcaaaga
    20521 aatagatggc gtaacataac ttaataaaat cagtgaacgc accgttttcc taaatctgaa
    20581 cttgcggtta agcaaaatag ctacggctaa tcctaaagct gtactgcctg ctacaactaa
    20641 gaatgtgtaa gcaactgtta aacctaaaga acgataaaat tcactatcac ttaaaatttc
    20701 aagataattg cttaatccga taaaaacggc gtctgagtta ggattcagcg gcatatcgaa
    20761 aaaactgatc tgaatattgg aaagcattgg aatcagcact aaaacagcta ataaaagcat
    20821 gcttggtgct aaaagtccca tggctaattt taagtcagaa tgtttccttg gacgattcat
    20881 tgaaacctcc ccctgataat ctgccaatag ctatctactt ttttcgaaca ccattggcag
    20941 aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga catcttcgtt
    21001 tccgacagtt acttggttca ctgcttttcc gatcacacct gagcttgtga tatctcccat
    21061 tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt acttcatcaa aagcactagc
    21121 aatctcagat ggcagctcgc caaaagactt gatgacttca ttttctttgt aaccatccag
    21181 ttcgactact tgtttattca ctggttgagc accaccagga gacatcaaga cccacttttc
    21241 catgtttttc ggttgagata agaaagctgt gaatgcttca gcagcctctt tttgtttctc
    21301 gtctagacca gaagaaatcg tcaatccaga tactgtgcca tagacagctt gatctttttg
    21361 tgttgggata gcaaaaccga tattttctga gttccccgct tcataaacag aaggcaaaat
    21421 gtaagttgaa tagattgcca ttggtgctgt tccattcatg aaagcatctt tgatttctgt
    21481 cacatcatta gagcctggca tcgtgtattg ggataagttt tgataaaacg acaatgcttc
    21541 tctcatcttt tccgtatcga tcgttacctc gccttcatca tccaaaacat ttgctccatt
    21601 tgacaaagca aattgagaga acgcttgctc actcattgtt ccttcagcag taggcatagc
    21661 aattccatat tgtttattct ctttatctgt gaaatgttga gcaattttta aaatatcatc
    21721 ccaattttct ggttctgaaa aaccagcttc tgataatttt tctttgtcat accaaatacc
    21781 ttgaacccag ccgctaattg gtgcagcaat atagcttttt ccatcttctg aacgaacaag
    21841 gtttttggct ccgtcataat aattatcttc tccagcttcc tcgataacgt tttggactgc
    21901 cgtctgatca atcagttcat ctttatccat cactttagca aaatcctgac tgacttccat
    21961 tacttctggt aatttaccgg aacgagctaa cgtaacgatt tttgtattga acgctgattc
    22021 ttctaccggc acttgcttga ttttgatgtc tggattttct ttttcaaaat cagcaaccag
    22081 ttcattgatc acacttaatc gttcctgttc gacagaagag tgcatgaatt gaatcgttgt
    22141 ctgttgtgat tcgttagagc tttgactttc accacaagca gtaagaagaa atccagtagc
    22201 tagcagacag gtagtaacaa taattttttt catttttttc cacctcttat tttattattt
    22261 tcagccacat atattcatac ggctggaata tcaactttcg ttcttgactg caatggataa
    22321 tttgtccaga aaacaggtct aggtaatctc cttttggtaa aagataacct gctgattgat
    22381 tggaaaaatt atggataact aagactttct cttccttcgc ttcgcgaata aaactgataa
    22441 aagaatctgc tgagtccagt aagtaaatca aactattcgg atggaaggct ttttcttttt
    22501 ttcgaataga tagcatctgg gtcaattcac catatactgc tgttcttaat ggatctgtct
    22561 gcaactcttt ctcaatatct gctaacaaaa atttttgccg attgattttt cgattctctc
    22621 ctgttttttc tacgcctttg taatcatttc gacttcccag cacactttga atataaattg
    22681 ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt ttgcttctcc
    22741 attccgtcgt atctgttgga gaacttaacg catctagata cgtagcgttt atctcatatg
    22801 gactggtcgt tccatctggg tttttcttgt agtttactcg ggcaccctct ttcttcagct
    22861 gttccactaa ttcaaggatc gttttttccg gcagaatacc tcgtaccgga ttcaatccga
    22921 taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg aaatcgatgg
    22981 tttttaacca ttctttcaac ttacttgtat tacccgaacg aatcgcttga agggtcaaag
    23041 gtggtagcga aaactgatag accatctgtg cttcattctc gccattccca aaataactga
    23101 tattatcttt gtgtggcaca tttgtctcag tgatcaatac cgttcctgga gctgcttcat
    23161 cagcgattgc acggaataat tggatgattt tatgcgtttc tggcaggtgt atacaagaag
    23221 ttcctagttc tttccataaa aatcccacag cgtccaagcg aataaatgaa gcgccttttt
    23281 ctaagtaaaa caaaagaata tcgatcattt tcaataaaac ttctggattt ttaaaattca
    23341 agtctacttg atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg tttttgtcaa
    23401 tgaacgttgt aagtaacggg ctggtccttg gtctagtcac gctgcttgta tctgtatcca
    23461 gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa gtttaaatat tcctgaaacc
    23521 aagtactttc ggcagacata tgattgcaga caaagtcgaa cattaaccgt gtttcgcttc
    23581 tcatttcttc tatatcttcc catgtgccca aacggtcgtt gattgcctga taatttacaa
    23641 cggagaaacc atcatcagat gtatatggga aaaatggcaa gaaatgtaca actctaaatg
    23701 tatctgccaa atacttttga taaaatttct taaatgtacg caaagttggt tgctgctctt
    23761 caaaaaattg atccccataa gtaatcaaga aaatatcttc ttcgtcccaa tgcagtttgc
    23821 ctgcctggcc tttcttctgc tgataaacac tgactttttg ttcaatcata gaggcggttt
    23881 tctcagacaa atctgagtcg cgataaatcg cttgtaaaag ttttgtatac atcggattcc
    23941 cctttctttt ttgggagcgt tcccaacgat ttgatcatat tatggaaaac gcttactgtc
    24001 aatagcattt ttttaaataa ttttgatata cacaaaaata caaataagaa agcttatgta
    24061 tcaataatta atagcttctt aaaaaaatat ggtctctcat gatcatgcaa gctattccta
    24121 aattttcaaa atcatagaca aaaaagaatg tctattgtac tatggaatta ttctaaaacg
    24181 ctcccaaaaa ggaggtgcat ataaaattga aacctactat ttatgacatt gcgaaaaaag
    24241 ctaatgtctc aaaatcaact gtctccagag tattaaataa ccagccaaat atctctgata
    24301 aagcaaaagc aagtgtactt caagcaatca aagaattgaa ttatgaaccg agcaaaattg
    24361 caagaggact gtcaggaaat ggttttgacg cgattctagc attatctcat cgtacttctc
    24421 atacgaccta tggcaatcct tttttttcag atgttattca atccatttct gccgtttctg
    24481 aacaaaataa cttcgacttg attctacaaa cggcaaaaac accagaagaa gagctagaaa
    24541 aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat tattatttta agctctacga
    24601 tggatgaaac acttcttttt gaattggatc aatataatat tcctattgtg gtaatcggaa
    24661 aagtcggaaa cgactacacg aacatctact cagtggacac ggataatttc caggatagtt
    24721 acgatttggt ggaaacactg atcaagaatg gacaccgtca tatcggctgt ctccatgctc
    24781 cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc ttatttgatc
    24841 accacctaga tatccgtaat gaatggatta tagacggcgg ctttcgctta gaagatagca
    24901 tggcagcaat gaatgattta atgaagctac gagaaagacc aactgcgatt ttttctaccg
    24961 atgcattaaa atcattgagt atttacaaag tggcagaaca atctggaaag cagatacctg
    25021 atgatctttc gatcgcagga ttcagtgatc ccttattgtc ctccctattt tcgccacagt
    25081 tgactcggat agacattccg acaaatcagt taggaatcaa agccacagaa cttttattcc
    25141 aactaatcca ccaccaatac cctgaagaaa aaagaatcat tctttcaacc gagattactc
    25201 tcacagattc gatccaacaa caagaaatgt agaacagaat tataaaagaa cagaaacact
    25261 gaggataagt cagttactct gttctttttt caatcactca tccctcgtta ccccctcccg
    25321 gtattttagc gaatttttct tattttccat ctgttttaga tagctgtgga taactataat
    25381 gtttgtgtta aactattttt tccacaggga agaaaaaact atttttaacc acgttgtgta
    25441 taagttttta tagttcgttt ttttaaacac atttaccctt atttcaatcc atgttatttg
    25501 gggatactta tctctaaaac ataaatagaa taacagacat gtctactggt tttgttttat
    25561 tcgaggaatt attcaataat agctagcaaa atattaaaaa taaccgctct atcttcccat
    25621 caatttattt tgcattcaaa aaatgatcta tctttacttt tgttgattga cttctcttgg
    25681 aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga tcctttccca
    25741 cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg tgacaataat
    25801 ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc gtcttctgca
    25861 gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac accgacttct
    25921 tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat cttacctata
    25981 ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc agcatccgtc
    26041 aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt ttctaatgat
    26101 gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc tgagactagc
    26161 gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg taagatatct
    26221 gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat tacttcgata
    26281 tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc acgaagcaag
    26341 tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc gattgatcct
    26401 tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat acgtgctgta
    26461 taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc tttggtacgt
    26521 gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat cactgcttgc
    26581 cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg agtgatatcg
    26641 tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc aggttcaccg
    26701 attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga gccagttgca
    26761 aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt gaagacagaa
    26821 cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc
    26881 aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc tttacgggtg
    26941 taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc gcggatggct
    27001 tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac atcttgggcc
    27061 acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc tgtatcggtc
    27121 atccctttac gagccccgtg agttgagata aacatttcca agacggatag tccttcacgg
    27181 aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat caaaccacgc
    27241 attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc agacatcatg
    27301 aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct gtcttttgca
    27361 gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag accacgacgg
    27421 aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc ttgtttttca
    27481 ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga atgtttgtac
    27541 cctaagtctt tcatacggtc aagcatctta gaagtttctg ttactttgaa tcgtttgaag
    27601 acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa ttcttgctct
    27661 ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg tgtttggacg
    27721 gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat gatctcgttg
    27781 aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc agtaaatggt
    27841 ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac atatccgttg
    27901 cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct
    27961 tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgagaagg agtaacaact
    28021 ggtttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat acgtgcttct
    28081 gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc gaaatcggca
    28141 ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac taaaactggt
    28201 tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt
    28261 tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc gattttacgt
    28321 ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat cacgaatggt
    28381 ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt caagaaaggt
    28441 ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa gttttgacgg
    28501 aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg gttacctggt
    28561 ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc ttcttgcagc
    28621 atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa acgtttcaaa
    28681 cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt tgcaaaacgt
    28741 ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat aacgtccata
    28801 accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg
    28861 attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa ttcggctgct
    28921 tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc catagcagct
    28981 tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt
    29041 tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa ataaatgatt
    29101 tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg aatcccttta
    29161 aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg ttcacggcga
    29221 actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt ataacggata
    29281 cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat ccgctcacag
    29341 aacaaccctt cacgttcagg ttttaaggta cgatagttaa tcgtctcagg tttttttact
    29401 tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg catactttcg
    29461 aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca aaccgcaaga
    29521 cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt atcttctgcg
    29581 gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc agctttcttt
    29641 tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac agtgatcaaa
    29701 tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat atccaagaca
    29761 cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga ttccggtaca
    29821 cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac acgtccgact
    29881 acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc gtaagcttcc
    29941 agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt acctcccaat
    30001 ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt gtcatcgacc
    30061 atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc aaatggttca
    30121 cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc ttcacgaact
    30181 gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac gtggatacct
    30241 aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt catacgtgaa
    30301 ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag gaatggcata
    30361 tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc ggccatttta
    30421 tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat gtttacgcct
    30481 ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac gataccgccg
    30541 ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc gccaaagatc
    30601 gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt gactttccct
    30661 acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat ttcgtctaaa
    30721 tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc aggtcctaat
    30781 tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta tacgtcatct
    30841 tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc ccaagtcatg
    30901 aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga tggtccgtct
    30961 gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg ttggttgtag
    31021 cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa tgcaccgttg
    31081 tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccgtc atgtttacat
    31141 aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc aacccatggt
    31201 gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat caaggcacgg
    31261 ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac tacttgttta
    31321 ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc actttgagca
    31381 cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg
    31441 gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc agtaacacga
    31501 cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt atttactttc
    31561 gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt ttcgatcgga
    31621 cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc gcggtcacga
    31681 gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc gccaagaggg
    31741 tttgtttgat ccatgaactg tgacaactga gaagaaccaa agaattcttt gatacttgcc
    31801 acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc ttggatcgac
    31861 attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg gttttgtaaa
    31921 agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc gacattgccg
    31981 ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc tggacgaacc
    32041 gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg ttcagggtct
    32101 cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac accgtcttct
    32161 gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag tgtttccatg
    32221 acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc tggatcaact
    32281 aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt tttgtttact
    32341 ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg agcattcagc
    32401 aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct
    32461 ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta
    32521 ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga accaaatcct
    32581 aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg tacataagag
    32641 atgtcttttg catcagtttc catttctagc caagctccac ggttagggat aacagtagaa
    32701 ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc tggtgaacgg
    32761 actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc
    32821 attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt ctcgcggttt
    32881 gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc acgagcttct
    32941 gctactgtat actttggttc tttcaattca taatctacaa attccaaaga tagattgcca
    33001 ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc atctaagaac
    33061 cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac ttcactgatt
    33121 cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc
    33181 ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca ataatatttc
    33241 aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa
    33301 aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt ccttatatta
    33361 aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt tttgcaactt
    33421 catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt ctgctttttc
    33481 gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat tcttaattga
    33541 cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa cttctatgcg
    33601 aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta gctactttag
    33661 ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta tctatgatgg
    33721 ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag
    33781 tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt ccgacttttc
    33841 aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa
    33901 gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc tttaggaggt
    33961 tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat tggcgtattg
    34021 caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga aggattagca
    34081 gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc ccaaaacaat
    34141 caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga tttgctactt
    34201 ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat cccgatgact
    34261 gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga aaaacctggc
    34321 ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg cttactgtta
    34381 aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga agcaaactct
    34441 aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta
    34501 ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc taaagatgta
    34561 gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa
    34621 gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt caaagacggc
    34681 ggtctagcaa cagtaggaat cgactacgaa agcttgggca aacaaacagg gatcatggca
    34741 gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc gaatgatttg
    34801 gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga cagcatcaaa
    34861 gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt ttcttatcat
    34921 ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc actttccgta
    34981 tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca gggatcgcgg
    35041 cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc tttttagggg
    35101 gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt cccgcccttc
    35161 ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg atgggtgcgc
    35221 cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca ttcggcataa
    35281 caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac
    35341 ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc gataatctgg
    35401 caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt tatatgattt
    35461 ctaatggatg cattgctctt tctggctcct tacttgcgca aaacaacggc tttgctgatt
    35521 taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc gcagaagttt
    35581 tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt gcggtcattt
    35641 atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat tcgaaacttg
    35701 cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa aaattccatt
    35761 tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat tggaaaatct
    35821 ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct
    35881 ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg gtaaatctac
    35941 tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt tattgaatgg
    36001 aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca
    36061 agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat
    36121 gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc gaacattttt
    36181 caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct cagaaatcgg
    36241 tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat tacagcgtcc
    36301 agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt ccaaaacggt
    36361 gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta tggtcactca
    36421 tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc aaggaaggat
    36481 cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat tattagcgtt
    36541 gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat cttcataatc
    36601 agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct ttccttcttt
    36661 cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc tctccttctt
    36721 cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca aggtacttca
    36781 attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc aactggaagt
    36841 ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct atcgttttga
    36901 gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa aagaaatcca
    36961 gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga cttctgaatc
    37021 tacgatgaaa gatgccaaac tggcagcagc tcaaggagat tctgtttcga agttatttgt
    37081 tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg
    37141 acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg aattgcctca
    37201 atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc
    37261 tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct gtgggatttt
    37321 atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca ttgggatcgg
    37381 gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa aagccatctc
    37441 tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca aaatctggca
    37501 agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata aagaaaaatc
    37561 attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc
    37621 aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga agatgagttt
    37681 gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag atttcacaaa
    37741 aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag actctcctta
    37801 ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa tatctataaa
    37861 gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat cagtttacac
    37921 gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc atgggtaacg
    37981 ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta gctagtgcct
    38041 taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg catagcagca
    38101 tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca gttctcgtca
    38161 attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc aaaatcagca
    38221 gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg ctttgcttgt
    38281 tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc
    38341 cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg gaaatctaat
    38401 tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc tctatcttcc
    38461 actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc aaaccgatcg
    38521 tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt tcctcctttt
    38581 tatttatcct acttactttt tcctaattct aggataccta cttatatgtt actacacacc
    38641 cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga aagaaaaaga
    38701 tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt aatattcttt
    38761 aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga gtataatagg
    38821 aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt actagtccta
    38881 gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga aacatacatt
    38941 cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt gttaggaatt
    39001 gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca tacagacgga
    39061 aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct tcatgaaggt
    39121 ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc
    39181 atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca
    39241 cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac tccagatgat
    39301 ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt tgctaaattg
    39361 aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac tgctgcagat
    39421 tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac cgggttttta
    39481 ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat cattaatcca
    39541 aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa cgtacagtca
    39601 ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga tgcgaatatc
    39661 tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt tcaaggaaca
    39721 atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac gattatagga
    39781 acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat atttatcagc
    39841 gacaatggta cagatacgac agacacaagc gacagtacag atacatctac cacaagtgat
    39901 agctcagatt catcaacttc aagcgacagt acagatacta cctctaccac aagtgatagc
    39961 acggatacat cagcttcaag cgacagtaca gatactacct ctaccacaag tgatagcacg
    40021 gacacatcag cttcaagtga cagtacagat actacctcta ccacaagtga tagcacggac
    40081 acgtcagctt caagcgacag tacggataca tctaccacaa caagtgatag cacggacacg
    40141 tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgatagctc agattcatct
    40201 accactacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga tacatcctct
    40261 accacaagtg atagctcaga ttcatcaact tcaagcgata gtacagacac gtcgtctacc
    40321 acaagcgata cacgtcatgc aagtgtctcc ttctcaaaaa caagttcgga taacaggacg
    40381 aactttaact taactaataa aacaagcaat gacaatggaa acaaatcaaa aagagcactt
    40441 cctaaaactg gaagtcaatc gaataattgg ataacactcg caggcgttat attacttgta
    40501 atcggtttaa gaatcacttt tatgagttat aagaaatcga aaaggtaaaa aaatattcaa
    40561 gataaaaaag agcaacgtcg ctaacggacg ttgctcttta tttttgcttt ttatcataaa
    40621 tacaagaact acaattcaat ttgtcaaaaa acacgtagat ttccttctga gaaatttatt
    40681 tacgtatcga acctttccct attcagtctt tgcgatccaa aatcagttgg gtaagggaaa
    40741 agagattctc ttttgttcga agagaggtgg caggtaattt ctttatttcc ttcaaagcct
    40801 tttctgtgta agaggccgct aacttcctag cttcttccac gcctcctgta tgttcaacga
    40861 ttttgcgtac ggcgtcgaca tcttcttctg tcattgccgc tttcttctct aaataaggta
    40921 gtagctttgg tttgtttttt tgcaaagagt agatcaacgg taaagaatag accccttgac
    40981 gcatgtcttc tagtacaggt ttgccgattt cctggctctc ttttgtataa tctaaaatat
    41041 catcgataat ctgaaaggcc atcccgatag aaagtccgat atctcctgct ttttttgcga
    41101 agcgctcgct tgttccactt tcaaaagcgc caacagaaca acttaattgg aacagttcag
    41161 ctgtcttacc tgaaatattt tccaaatatt cttttacaga aagttcataa ttatagcgat
    41221 catccatctg ccctaattcg ccattcaata ctttttccat actgcgagaa ttcaattgga
    41281 gacttttcaa ggaagtggag taatcagaca atagtttaaa acagcagaca aataaatagt
    41341 ctcctgcata gacagaaacg acattaccaa actgactacg gatattaggc tgaccacgtc
    41401 gcagatccgc ttcatcaacg atatcatcat ggatcagtgt ggccgtatgg agcatttcga
    41461 tag
//
LOCUS       DAZKBH010000011        41120 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000011 DAZKBH010000000
VERSION     DAZKBH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.011"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(406..1188)
                     /locus_tag="ACG8OO_000620"
     CDS             complement(406..1188)
                     /locus_tag="ACG8OO_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044551.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1253..2089)
                     /locus_tag="ACG8OO_000621"
     CDS             complement(1253..2089)
                     /locus_tag="ACG8OO_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9044552.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2107..3516)
                     /locus_tag="ACG8OO_000622"
     CDS             complement(2107..3516)
                     /locus_tag="ACG8OO_000622"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9044553.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3530..5353)
                     /locus_tag="ACG8OO_000623"
     CDS             complement(3530..5353)
                     /locus_tag="ACG8OO_000623"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9044554.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6196..6522)
                     /locus_tag="ACG8OO_000624"
     CDS             complement(6196..6522)
                     /locus_tag="ACG8OO_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044555.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6568..6930)
                     /locus_tag="ACG8OO_000625"
     CDS             complement(6568..6930)
                     /locus_tag="ACG8OO_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFP9044556.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6967..7182)
                     /locus_tag="ACG8OO_000626"
     CDS             complement(6967..7182)
                     /locus_tag="ACG8OO_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9044557.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7309..7644
                     /locus_tag="ACG8OO_000627"
     CDS             7309..7644
                     /locus_tag="ACG8OO_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9044558.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7720..9717)
                     /locus_tag="ACG8OO_000628"
     CDS             complement(7720..9717)
                     /locus_tag="ACG8OO_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044559.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9815..10090)
                     /locus_tag="ACG8OO_000629"
     CDS             complement(9815..10090)
                     /locus_tag="ACG8OO_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9044560.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10322..11056
                     /locus_tag="ACG8OO_000630"
     CDS             10322..11056
                     /locus_tag="ACG8OO_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9044561.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11095..12237
                     /locus_tag="ACG8OO_000631"
     CDS             11095..12237
                     /locus_tag="ACG8OO_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9044562.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12332..13723)
                     /gene="sufB"
                     /locus_tag="ACG8OO_000632"
     CDS             complement(12332..13723)
                     /gene="sufB"
                     /locus_tag="ACG8OO_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9044563.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13728..14198)
                     /gene="sufU"
                     /locus_tag="ACG8OO_000633"
     CDS             complement(13728..14198)
                     /gene="sufU"
                     /locus_tag="ACG8OO_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFP9044564.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14185..15420)
                     /locus_tag="ACG8OO_000634"
     CDS             complement(14185..15420)
                     /locus_tag="ACG8OO_000634"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9044565.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15417..16703)
                     /gene="sufD"
                     /locus_tag="ACG8OO_000635"
     CDS             complement(15417..16703)
                     /gene="sufD"
                     /locus_tag="ACG8OO_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9044566.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16716..17489)
                     /gene="sufC"
                     /locus_tag="ACG8OO_000636"
     CDS             complement(16716..17489)
                     /gene="sufC"
                     /locus_tag="ACG8OO_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9044567.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17686..18534)
                     /locus_tag="ACG8OO_000637"
     CDS             complement(17686..18534)
                     /locus_tag="ACG8OO_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9044568.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18558..19244)
                     /locus_tag="ACG8OO_000638"
     CDS             complement(18558..19244)
                     /locus_tag="ACG8OO_000638"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9044569.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19237..20271)
                     /locus_tag="ACG8OO_000639"
     CDS             complement(19237..20271)
                     /locus_tag="ACG8OO_000639"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9044570.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20334..20590)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20666..21004)
                     /locus_tag="ACG8OO_000640"
     CDS             complement(20666..21004)
                     /locus_tag="ACG8OO_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFP9044571.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21006..21362)
                     /locus_tag="ACG8OO_000641"
     CDS             complement(21006..21362)
                     /locus_tag="ACG8OO_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFP9044572.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21368..22555)
                     /locus_tag="ACG8OO_000642"
     CDS             complement(21368..22555)
                     /locus_tag="ACG8OO_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9044573.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22746..23591)
                     /locus_tag="ACG8OO_000643"
     CDS             complement(22746..23591)
                     /locus_tag="ACG8OO_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9044574.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23825..24790
                     /locus_tag="ACG8OO_000644"
     CDS             23825..24790
                     /locus_tag="ACG8OO_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFP9044575.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25167..26210
                     /locus_tag="ACG8OO_000645"
     CDS             25167..26210
                     /locus_tag="ACG8OO_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFP9044576.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26272..26736)
                     /locus_tag="ACG8OO_000646"
     CDS             complement(26272..26736)
                     /locus_tag="ACG8OO_000646"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9044577.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            26982..27929
                     /locus_tag="ACG8OO_000647"
     CDS             26982..27929
                     /locus_tag="ACG8OO_000647"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9044578.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28140..30212)
                     /locus_tag="ACG8OO_000648"
     CDS             complement(28140..30212)
                     /locus_tag="ACG8OO_000648"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9044579.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30145..30522)
                     /locus_tag="ACG8OO_000649"
     CDS             complement(30145..30522)
                     /locus_tag="ACG8OO_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFP9044580.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30651..31211)
                     /locus_tag="ACG8OO_000650"
     CDS             complement(30651..31211)
                     /locus_tag="ACG8OO_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9044581.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31361..32653
                     /locus_tag="ACG8OO_000651"
     CDS             31361..32653
                     /locus_tag="ACG8OO_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9044582.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32713..33831)
                     /locus_tag="ACG8OO_000652"
     CDS             complement(32713..33831)
                     /locus_tag="ACG8OO_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFP9044583.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33845..35266)
                     /gene="arcD"
                     /locus_tag="ACG8OO_000653"
     CDS             complement(33845..35266)
                     /gene="arcD"
                     /locus_tag="ACG8OO_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFP9044584.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35512..36633)
                     /gene="mnmA"
                     /locus_tag="ACG8OO_000654"
     CDS             complement(35512..36633)
                     /gene="mnmA"
                     /locus_tag="ACG8OO_000654"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9044585.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37037..37636
                     /locus_tag="ACG8OO_000655"
     CDS             37037..37636
                     /locus_tag="ACG8OO_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9044586.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37774..39060)
                     /gene="efmA"
                     /locus_tag="ACG8OO_000656"
     CDS             complement(37774..39060)
                     /gene="efmA"
                     /locus_tag="ACG8OO_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFP9044587.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39331..39675)
                     /locus_tag="ACG8OO_000657"
     CDS             complement(39331..39675)
                     /locus_tag="ACG8OO_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFP9044588.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39812..40957)
                     /locus_tag="ACG8OO_000658"
     CDS             complement(39812..40957)
                     /locus_tag="ACG8OO_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9044589.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11824 a         8646 c         7189 g        13461 t
ORIGIN      
        1 ctaataaact acgcgaaagg agtctaacgt tgtttgatat aacttagact gatcgttaac
       61 ttatttttta tatttctaca tagttatctt tagaaccatt tcagatacat atgaaacaat
      121 tacatatgat tttttcataa aaaaacagta ctgtctagta gtataaaata tacaaatttc
      181 ttaaaaattt tttcactaaa atcactacct gaaacagtct aaaacactta tatatcaacg
      241 tttaatgaaa ctatctccgt ttcatttcag atacaaataa atatagtcgg gctccaaatc
      301 cgaaaaaaaa tacccccagt agcttatcct aagctactgg agatgtataa gtacggtcat
      361 ataacaacgg ggtagggtaa ataaatgaaa aagatggaag aatcattatt caataacttt
      421 tttaaatact tcgttgtaat cagttacctg tccgttctta aataccacag aataatcttt
      481 tttcccattt tcaagtaacc gaagtggaat gtctttacca acttgctctg tcactgcatt
      541 actgatacct gttagattag ctaataaatc tgataaagat tgagcatctg tagaaatctc
      601 tgtatcagta tttatcgtga ttcctttatc ttcatttaca agcttccaag taggtggtat
      661 agctgtgtat gtttcgatga atgaatcaat tctttcatct atcgtcttcc cagtatctac
      721 ttctgtctca ttactcttag atgaatcgat tctttcatcc atcgtctgct ccgtaactgc
      781 ttctgtctca ttattctcac cggaaactct cttcacaccc aaacttatat attgtccatc
      841 tttagtgtgc atagttaaaa ttcccacgct tttactaata aattcactat aaccagttct
      901 ttcaatagag acttcaatgt atttcacact atcatactca aaagaaaaat agacatgtaa
      961 aagcattttg ttgtttacat ctacataata atcctttgac tctacttgag accttcctat
     1021 acggaataca gctttactgt ctgatatctc tataccagca tccttattat taatctgcca
     1081 atctccaagt agctcttctg gcaagctagt agtttcttgt gaacacttta ccacttcact
     1141 ggtagaggtc gaaacattca cctccgcact gttagaagaa ttttccaagg aatcaggagc
     1201 cttagcacaa ccaacaagtc tttatatatt gtgcagccga tattttcttc attcagtaca
     1261 cccttgaaat atgcagtgtt agataaagca gttcgtcctg atttgctgag gcacccatct
     1321 cttcttgaac gtactccttg attttcaagg ccgcccgata ctctttcgga aaacgctgct
     1381 tcactgaagc cagcaaaaaa tcatccttct ccggcaactc ttccttctta atcattcggg
     1441 caataaaaaa ctgtaaatgc gtcacaaatc ttaagtaaag catcgaatct ttatcaacct
     1501 ttttttcggg aaacagatat cctagcatag ccaagatatc ctttagtgct cttaccgaga
     1561 gcatggtttc ttccatattt gcttgttcac tttgggcatt tacaatgtga aacgcaatgt
     1621 ttccagcctc ctcttctggc aactgaacat tcattttttt gttgatcaat tggatagcat
     1681 agcagccgat ctcaaattct tttgggtaat acttttgaat ttcaatcagc aggcgatttt
     1741 gaaaaacaat gcctttttga ctgcgttcaa tggcatgctt caagtgatca accaaggtaa
     1801 aaaatagact tttattcagc ttaaagtcaa acttggcgtt ggcataatcg ataattcgct
     1861 tgacgcaaat aattagatca tccggagcag cctcaaacag tttaatgtaa tcctttttta
     1921 gagcatcaga aagtgaatct aatttaaata ctttttggat tttgtcagcc ggaaccaaat
     1981 cgcctttctt tagttgaaat ccaatccctt tccccatcaa aattacttct ttgccttgct
     2041 tatctaagca aagaattaaa ctgttgttta acgttttaat tactctcatt agattcaccg
     2101 ttctctctaa gctaagccat ttcttgcgat tactttttga taccaaaaga agctttcttt
     2161 ttttattcgt tttaattctt taagattctc atcggttcgg tcaacatata taaaaccata
     2221 acgcttattg aagccttgat gggtactaac aacatcaatc gctgaccaag gtaaataacc
     2281 aaaaacatct acaccatctg ttaaagctgc ttgaatagcc agaatatgtt tggctagata
     2341 atcaattcga tcctggtcat taattcgtct atcttcctca actatgtcgg gtgccccata
     2401 gccattttca gtaattaaaa taggtaaatc atagcgatcg tacaatttcc tcaatgttaa
     2461 tcgtagtcca gtagggtcaa ctacccagcc atatggcgtt ttatctacat aaggattttc
     2521 agctgcccgg tagacacctt cttctcccaa catgatctgt tgatcccccg ctctagcgga
     2581 aacatctgat ccgtcatttt tgctggcagc gatggtggca gttgaataat aattaatcgc
     2641 aataaaatcg ggtttaccgg aggataaaat tgcctgatcc tctgcctcaa ttttaggata
     2701 taatccacga tcattcaaat agttttcaaa caaataattg tattttcccc gaactgctac
     2761 atctaagaaa gaccagcctc tcaatgtttc ccaattatgg gctgcaattg catcttctgg
     2821 actggaggta gcttgataca tagccgttaa attgattgct ggaccgattt ttcctttaag
     2881 attcaactga tgatacaagt gaattactct agcttgagct aataaaacat ggtgattaat
     2941 ttgaaagagt tctttttttg aaggcagctc tccacttggt ggcaaaccaa tcgctccagg
     3001 atgaaggatc atcgtgtttt gctcattaat tgtcagccaa tagttcacct tgtcaccata
     3061 ctcagtaagc aatacctgta catagtccac aaaataatcg atagttgctc gatttaacca
     3121 accgccctgc tcctctagaa aataaggtaa atcgaaatgg tacatggtga caatcggttc
     3181 gattccctga ttcaggcatt catcaattac ttgatgataa aaagaaattc ccttttgatt
     3241 ggttttatta ttttcaatat ccataattcg cgtccaagcg atggaaaagc gaaaagcttt
     3301 taagcctaat tctttaaaca gctgtatatc ttctttatag tgatggtaaa aatccgaggc
     3361 aacttcaaaa ttagctaccc cttctgtatg ctgatacttg tcaataatag acggttgttt
     3421 cccatcctcc tgataggcac cctctgcctg gtaggcacta atggaggcac cccataaaaa
     3481 cttctctgga aatgctgaaa gctgtttgta ttgcacaaaa atcctctcct taactaattt
     3541 gcatcaatgg ttcgtgttcc tttaagcttc cgcttgcttc actaattcct gcgtaatttt
     3601 cactgttggt cactaccaca atcactgtat catcgtagcc agcgtttcta atggcagcga
     3661 aatcggcgtt gattaataat tgtcctgctg acacctgatc tcctgctttt acaaatactt
     3721 caaagggttc cccttttaaa ttcacggtgt cgatccccac atggatcaat aattcaatac
     3781 cttctttaga acgtagacca atcgcatgtt tagattctaa aatagtaatg acttctccct
     3841 caacaggtga ataaatgctg ttttcttctg gatgaatggc tacgcccttt cccaatagtc
     3901 ccttagaaaa gacagcatcg ttcacttcag aaataggaca cacggttcct tttaccggat
     3961 tttcaacaat gactgcttcg gctattgccg tttgctctga ttcatcagaa gtatcgtcaa
     4021 atccaattag ataagcaatg ataaaggttg caacaaaggc gattacacac gtaataattg
     4081 cgtgaacaat attcattgga ttttctccaa taaaggctgg taaggctgct aagccagggg
     4141 aaacaaaggc atatctgact aaaccgctca agccagcata caaaccagca attgcaccac
     4201 caatcatgga agcaactaat ggttttttca gcttcaaggt tactccatag agtgaaggct
     4261 cagtaattcc cattaaagca gtgatcccag tggacacagc tagctgcttc aactctttgt
     4321 ttttagtttt cgcggctaca gcaaaggtag cacctgattg agcaatatta gaagccagca
     4381 ttcctggccc attgatcatt tcagaacccg tactgctcaa ttgcatagta gcgattggcg
     4441 tcattgccca agctgtaccc gtaaccacca aaaatggctg caaaccaccc atcaaaaatg
     4501 gaattaacca agctgctttg ccgttaatcc attcagcccc cattgctata agatcgttca
     4561 gcaacgttcc aaacggtcca atgacaacca acgctaaagg ccccataatt aaaaatacca
     4621 gcatcggctt agtaaagaat ttaataaacg aaggcgaaat tcgatccgca aagggctcta
     4681 cataggacat gatccaaaca gtaaagataa tcggtaaaac tgatcccgca taatctgcag
     4741 ctaataccgg aattcctaga aaagaaacgg catcgccagt agaaaaaagc tgtgaaatat
     4801 ttggatgaag caaaatgcca gcaacagtaa tcgccaaaat tgggttgaca tcaaacttta
     4861 tggctgcccc ataggctaat agtactggca taaaataaaa ggctgcatca gcaatagtat
     4921 tcaaaatgat gtaagtttga ctactgtctg atagtacacc agtcaaagtc aagacggcca
     4981 aaatggcttt taccatcccg gcacctgtta tagctggaat catcggtgta aaggtggtag
     5041 aaatgacact aatcaatctt gaaataatac tttcttgatt gtctgaagag gggccctttt
     5101 taattgaagt tcccacttcc ttttgaattt ccttgaaggc tgtttggact tcattgccaa
     5161 tcaccacttg gtattgtcca ccgctatcca ccacactaat tacaccaagt gttttttcta
     5221 aggctttcgt atcggctttc cttttatctt tgagttcaaa acgcagtctg gtggcacaat
     5281 gggttaagct gaccacattt tcttttcccc caacctgttg caatacattt cttcctactt
     5341 ctctgtaatc cattattctc tctccttaaa gcagtaaaaa agaccaagtc aaaccacgcc
     5401 ccaatcaaca ttgaagtgtt agtttgtctt ggtcttgcct gcataatcag taacatgcca
     5461 ataacgttat tttgctgtaa tccaataata gcacattatg atagcgcaat caacagggaa
     5521 tttaattttt tatcttttcg tttgaccgta tagggtaata taacagttag atcacatcaa
     5581 ctagggtata tctgcgtata cttactgcta aaataagtat atctaatatt aactatacca
     5641 gtaaaaccat tcctacataa ccagcgggaa attcccaaaa tcgtgaaagc atcaaaaatg
     5701 catataacgg atcacacaac ttcaacaatt ctgctcttgg gaaaaattga tgaatttggt
     5761 gagtaaagga tggaataaac agcattccaa caatataggc aacaatagtg atacttgttt
     5821 ttttggtaaa aatcagtaag caatagccaa tttcaatcat tccgagatat aaaggtatcg
     5881 atagaatgat ttgggctagt attcttaaaa attaactagc aaatgtttaa actgatttat
     5941 ttctaatcta catgtagtaa gtatttcaat tccaccaatt atcaataaaa ataccaaaca
     6001 tatccataat aattttttct ttttcatttc ttttatattg ttataacagt gaataacata
     6061 aaaattcccg acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag
     6121 gaaactataa aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg
     6181 cataatacat ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat
     6241 acggtctttc tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat
     6301 agctttagcc cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga
     6361 tcgatttaag ttgtactgtt cttgtaaaat ctgtgttacc aatggttttg atgacttctc
     6421 taatccagat gtacctgtca tttttgaaaa aataatacct ataatagcca agacaacaat
     6481 acctataatg acaaacttct tgttcatatt tactttattc aactttttat ctcctatctc
     6541 caaaataatt tgctcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa
     6601 gattgctcca gctacccctg ctatcatcaa aatcgtaccc attttctcat ctttttctcg
     6661 atataagtag cccataataa cagcaacagc accaaataca acaggaataa atgctaacga
     6721 tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac
     6781 gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg tgtctgttcc
     6841 ggttgtctca tctgtttctt gtagtgctcc acaaaactgg caatacttac tttcatctgt
     6901 tatttctttg ccacattttc tacaatacat gtcttctcct cctaaaatca ttcaattgct
     6961 tattagttaa ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact
     7021 taatatctca gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca
     7081 cgaacttaca cattgctgtg taacattcat tttttttgct aactccgttt gagaaagtcc
     7141 tttttctttt ctgtatcggc tgagattact tacttttttc atgattctcc cctcctgttt
     7201 tattatatac aacaatatga tgtatttcaa ctcataaaat ataaaaaact tcaaatacca
     7261 ttacaaatac aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa
     7321 caatatgcaa aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta
     7381 gctgaaaaaa tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag
     7441 cctaacctcg atagcttaac agctctttct agagccctag gaacccctgt aagccatttt
     7501 ctagacaatg ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc
     7561 actgaagatg cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa
     7621 cgcaatcagc tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt
     7681 attgagatga aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc
     7741 ttttctcttc cttactctcc gtccatcatc tcttgttaca cttgtaaatt gatacagagg
     7801 aactatccca gctcctgata atatcaattc ttttattgtt tcatctatcc ctgccccttt
     7861 cgctaaatat attaaaaaac tactacttgc tttcacattg tatagtttct taatatcctt
     7921 agaagctcgt tcaagagtga atccttgttt aacaaagtag cctctgtttt ttattaaagc
     7981 atttttcacg ctgttatccc caacattacg taggcgttca caaacagtgt taataagttc
     8041 tttctctgtc attagatatg tcccttgcca cgataagccc tgtaaaactt catgtgcttc
     8101 atctaaaatc aattgttttt ccttgctgag ttctgatcgg gaattttgag gaaatactct
     8161 tttagcctct ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt
     8221 aaaatgaatt ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt
     8281 tgcattaaaa acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc
     8341 tttctcttcc tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc
     8401 taattttttt atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat
     8461 gtcataagtt ttaccatagt aagaactcat ttttttagct aattgttccc ccgacaaaat
     8521 acaagtcaca ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata
     8581 gacacgtcgc atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga
     8641 ttcaagagtg tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata
     8701 caagtctagg ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat
     8761 tagcatcaca actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa
     8821 aatggaatgc ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa
     8881 cagacatctt aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct
     8941 ttcataaatc tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg
     9001 tttcatttct tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag
     9061 ttcatactct ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat
     9121 cggaagctca gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt
     9181 atatcctcca aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt
     9241 ctttttcaag tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa
     9301 tagaaagaca atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc
     9361 aaacgcagaa ttatgaacta aattgataga taaaaccttt tctacgctta agtatctact
     9421 atcatcttta ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc
     9481 cagaataatt actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt
     9541 gcttggaata aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc
     9601 tctatagtta tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt
     9661 ctctaataat ttttcttgat ctacataaat tggaatatta atcattgatg tttccatttg
     9721 tgtttcctcc ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc
     9781 tttattacta tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac
     9841 tatcaatatc agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt
     9901 attgagaaac atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct
     9961 gatagtccat atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca
    10021 ctgtaattat ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt
    10081 gtagagacat atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct
    10141 tcgtattttt tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat
    10201 gattaccagt tcatcgacat acacataata cctcgataaa aaaggggtgt caacagcttt
    10261 gtaaaattta tttgcaacac cccttttttg aggtatacta attttaggag gtgtggtcaa
    10321 tatgataaaa cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat
    10381 aaaagctata agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa
    10441 cggtcaatta attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat
    10501 accagagcga ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg
    10561 tggcaatgta gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga
    10621 tggtataaca tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt
    10681 acactctaat ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga
    10741 atttattgct aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat
    10801 cttaaaaata gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct
    10861 tttcctcact agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat
    10921 tcaattgaaa gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa
    10981 caagctagaa caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg
    11041 aggtgatacc atctagtggt aagtactcct tgtaaactaa gaggagatga tttaatggca
    11101 aaaatatcaa agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt
    11161 gccaccgatc cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa
    11221 caagaagctc aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag
    11281 aaagcacctg atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc
    11341 agtgttaaag cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca
    11401 gcatttggta agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat
    11461 gaatggcaca gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta
    11521 cgctacgggg ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca
    11581 cgaaaaaaag agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta
    11641 ctggatgctt ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca
    11701 tacacaggta tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt
    11761 aataaagaac tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt
    11821 caatctccga aaacaagagc ttctagacga gttatctctt tagatactga gactctttct
    11881 atcctcaaca actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact
    11941 agctctaaag agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact
    12001 gttaatgatt ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat
    12061 gggtttagac atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt
    12121 caacaacgat tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca
    12181 aataatgcac ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag
    12241 agctggtcaa aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta
    12301 tatatcaagg gttctagctc ctataacagg tttacccaac actgccttcc atctcataac
    12361 tgatcaatcg attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa
    12421 caaagcccat gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat
    12481 agtagagttg ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc
    12541 tgttgtggat ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg
    12601 tgtcacattc gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc
    12661 gatagtttac ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat
    12721 ttggtgcatt gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg
    12781 cgatggagag catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca
    12841 tcgttgtttt tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt
    12901 ttgctcgttt ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac
    12961 ggcaatacgc atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg
    13021 aataagtagg tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga
    13081 tcagcgtacg ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg
    13141 gaacatcgac tttgacgcct tttggcacgt aaataaaggt cccacctgac catacggctg
    13201 aattcagcgc tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga
    13261 ataattcagg gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt
    13321 cgaattcatc tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg
    13381 ctagataagc tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt
    13441 ctggtacgtc ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg
    13501 catcgaaatc gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg
    13561 tttctagaga tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca
    13621 tctcacggat cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat
    13681 cgtgaaaccc gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc
    13741 ttgtggtaat gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc
    13801 caggcaagtg tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca
    13861 gcatcgccta attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct
    13921 acagttaaat gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag
    13981 gcacttgccg tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg
    14041 tcttttacat cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc
    14101 ttgcttgtcg agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc
    14161 acttgacggt ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc
    14221 catgattgct tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc
    14281 gcgagcagtc gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc
    14341 tctgacggct accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc
    14401 caaattgaat gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa
    14461 tccatcaatt gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg
    14521 gatattgtcc ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc
    14581 ggcgatattt ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta
    14641 taattcgaca aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca
    14701 ttttcgttta ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa
    14761 agcaaagaaa tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc
    14821 accatctact acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg
    14881 attgaagaca cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt
    14941 aatctttttc gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt
    15001 caagacagca cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc
    15061 catgtaagag agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc
    15121 tacccaattc aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc
    15181 atggatgaac gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc
    15241 taacgtatgg acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc
    15301 ctggagtact tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc
    15361 atcattgacg tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat
    15421 ccattcaact tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt
    15481 gctgttaaga cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc
    15541 aatcctctac tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca
    15601 tgtccagcag ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct
    15661 ttatcagaaa gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc
    15721 aagatgtggc cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc
    15781 aaaatatgcc cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt
    15841 tgtctgcccg agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa
    15901 tctgagtcaa agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca
    15961 gaagcatctc gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat
    16021 tgatctacgg ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata
    16081 ttacctgatg attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat
    16141 tcactatgtt catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca
    16201 tcttgaatga ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg
    16261 aaaataccgc tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt
    16321 actgcttttt tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac
    16381 aagtcggtaa aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc
    16441 catgaacctt gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg
    16501 cctgtttcac tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc
    16561 acccgatcga tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag
    16621 gttctcatcc aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa
    16681 tcgagccagt ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg
    16741 attcctaatt ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca
    16801 gcattccctg tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata
    16861 tagttcaata atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt
    16921 tcatttactc ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg
    16981 tctaaaattg caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt
    17041 tctccgccag aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt
    17101 aaagccattt tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca
    17161 tcacgtttcg cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca
    17221 cttggatatt gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct
    17281 aaaacatttt tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc
    17341 atgatagcgg cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt
    17401 tctccggttt tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca
    17461 tgtagatttt ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc
    17521 actcatttta gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc
    17581 ttttatttat tattaaacaa ttttgatttc taaattagaa taggtataaa aaagatgaga
    17641 aataagccat tacagactta tttctcatct tttttgttcc ataccttatt ttgcatcagc
    17701 ctttactggt ttaacagagc cgccccattt gtctgtgatc cattcttgga cttctttgcg
    17761 gtgaagcact tcaatcagtt ttttgatttt gtcactgtct tcatcacctt tacgagtcgc
    17821 aataatattc acgtaaggag agttgtcctt ttcaagcaat acggcatctt ctaatggatt
    17881 caaccctgcg ccgtaagcga agtttgcgtt gattgcaact aggtcacctt catcattttg
    17941 gtacgtagaa gctaaaagtg atgggtcgat cgtatggttg aacttcaagt tcttagggtt
    18001 ttcttcaata tcatcgaatg ttgctgtttc cagatctact ccatctttga ctttgactaa
    18061 gccagcatct tgtagaattg tgatgatgcg tccccagtct gattcagaat ttgaagtaat
    18121 gattgttgca ccatctttta attcggaaac atctttgact tttttagagt acaagcccat
    18181 tggttcgatg tggacagcac cggcattgac aaagtcatat cctttttctt tgatttcttt
    18241 gttcaaataa ggaatatgct ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg
    18301 gttcggtaat acatagtcat cgaatttgac gatttcaaga tcgattcctt catctttcaa
    18361 taatggtttc acatgttcca aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa
    18421 tgtcgtgtcg tctgctttat ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc
    18481 taatcctaca gtcagcaaaa gcgctgctgc aaatccaaaa attttctttt tcatatccta
    18541 catcccccta tatcctatta tcttttgtct gttttcttag ttaagaaatc accgattcct
    18601 tgaacgataa atacgattag taaaataatt aaagtcgcaa cgaatgtcac tgttagatta
    18661 ccacgctggt agccttcctg ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg
    18721 gccatcgcag taaatccgat catcgagatc gttgtaactg ttaatccaga aatcaatgcg
    18781 ggaatacttt caggtatcaa gaccttccag atgatttccc agtaacttgc tcccattgca
    18841 tctgctgctt ccagtacacc agggctgact tcacgaaaag caatctctac tagacgggca
    18901 taaaatggtg ctgcggataa aacaagagct ggaatggctg cttgcgcacc tagcatagtg
    18961 cctacgattg ctttagtaaa tggaatgatc aaaaccatca ggatcataaa tggcgttgac
    19021 cggaaaatat tactgatgat cgatacgatt ccataaatga ttcgagcata cggtttgttt
    19081 ttgcgtccaa tagagtaaag gatcaatccc aaaactaatc cgatgatcgt cactaagacc
    19141 attgagatta atgtcatata tagtgtatca aggatagcct gattcatcgt ttccgggttg
    19201 atttgtttaa aatctagata tgtttctgaa aagcttttat ccacgatgga tcacctcaat
    19261 tcctactcct tgttctttga tgaacacttc tgcttgatct aattctttgg tttcaccagt
    19321 catcaagaca gtcaatgaac caaaggaacc ttctttcact tgtttgatct ttccatacac
    19381 aacgttgaca tcgatgttat agcgtcgaat cgcttgggaa atgactggct cgttggcatt
    19441 tctttcgcta aaggtcaaag ttgccaaacg gccgcttgga ttttctttga tgagttcagc
    19501 taatagttct gtcgaatcag cttctggttc gatgtcttgt tgtacaaagc gtttggttac
    19561 ttcttttttc ggttgtctga agacagtcaa tacgtctcct tcttctacga ctttccccaa
    19621 ctccatcaca gcgactttgt tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa
    19681 gacgatggtc aaattcaatt tcttattgat cgctaacaat aagtctaata cttcttctgt
    19741 tgtttgcggg tccaatgcgc tagtagcctc atcacaaagc aggatctcag gatcatttgc
    19801 caatgctcgt gcgataccga cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc
    19861 ttgtccgcgt ccttctaacc cgactaaacg caacagttct tctgcccgtt ctttacgttt
    19921 gctctttggt acgccagcta attccagcgg cagctggata ttttcaagta cagttcttga
    19981 ccataacagg ttgaaatgct ggaagatcat accgattttc tttcggaatg tacgtaattc
    20041 tttgtttttt aattttgtaa tatcttgatc gtttacgatc acttcgccgc tcgtgggaag
    20101 ctcaagtcca ttcaggagac ggactaatgt actttttcct gcgccagaat agccgacgat
    20161 tccataaata tccccttttt caacgtttag tgaaacgtca ttgacggctt ggatgtttcc
    20221 gtttttccca ttgaatgtct ttttgacatt cgttagttgg attaatgaca tgccattcac
    20281 atctttctct tatgattttt caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg
    20341 acgaaagctt gaacttccgt gttaccacct tgatttaccg ttacttcgca ataacagcct
    20401 cttccagtac ttggacttag cttctttccg tatactgtcg cgctgtaacg ggcactcccg
    20461 tagtaagttt gcttacgctc gcttactctg ctctaagacc atcttccatc tcattttcgt
    20521 tacccatttt cagcaaacag ggctctcttc aaacgtccgt gacatgtact cttcttttca
    20581 aagctttctc gtttcatata ggattgctta tgattatagc aactggaaga atcagtgtca
    20641 acttttttca agtgattttc tgattttata attcgtcaaa ttcttttgga tcaacatctt
    20701 tgaaacttaa gatccacgca ttcatttcat ctgaatcatt caagatattg atatcttcac
    20761 cgattttatc attaacagaa gaaactgtac ccgttagcgg gctggcaaat tcattgaccg
    20821 ctttttccgc ttcaacttcg atcagaggtt catctttttt tagcgattgt cctgcttttg
    20881 gcagattggc aaacgtgatg tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac
    20941 agtattcttt tccattaaat aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg
    21001 ccattttata tcagttctcc ttcataaatt ttttcattga atccaattgc aacaagctgg
    21061 tcctctcgta agatcagtgg tcgcttgatc agcataccat ctgtcgataa gacttggctc
    21121 gcttttgaag ccgtaaactc gcctacttga tctttcagtc caagttcccg atatttcatc
    21181 ccgcttgtgt tgaacaggcg acggattggt acttgactcg tttctagcca agtctcaatc
    21241 gttgaggcct tcggtacttc tttgatcata tcaacggttt gataactaac gccatgctgg
    21301 tctaaccacg ctttggcttt tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac
    21361 aattttctta cctccttgaa gatcttgcag gtgcttcatt ttggtatctc atcgataata
    21421 tcaacccgat accgatcatg ttccctaaaa tcgaggatcc tccctgacta atgaaaggca
    21481 atgggatccc tgttaaagga agtaatccga tatttgctcc aatattctca aagacatgga
    21541 acagcagcat catgatcaaa ccgcttgcga tataggcgta aaattcatta ttcgtatcga
    21601 aacacacccg aatcatacga tagattaaaa taaaatacaa gaaaatgacg aatgcaccac
    21661 cgataaagcc gaagttttca ccgataacgg taaaaatcat atcggactca cgaacaggca
    21721 catagatatt gcttacgttg aatcctttac caaacaagcc accggtacca attgccaaaa
    21781 gtccttgggc cggctgataa ctcattcctg acgtatcatg gaaagggtcc agccatgaat
    21841 caatacgtgc aaactgataa gatttgaatc caactttata aagcaagtct cttccgcctt
    21901 cagtcgtaac caagaagatc gttcctgctc caacgactac tgccaagatt actacaggtg
    21961 cgatgatgcg ccaagagatt ccagacatca ggaaaatccc gccaaagatc gcaagaaaga
    22021 caagcatcgt tccaaagtca tcttgggcca tgatcaatgc taaaaccggt attgtcacga
    22081 tcaagagctt cccaatcagc caaaagtccg atttcatcgt ccgttctttt acttgcgtat
    22141 tatgtttagt tacgatcagt gccatcatca agatgtacgc aatcttcatc aactctgctg
    22201 gctggaaagt aaacgcaccg aatcgaaacc agtttttaga ccctgttgaa gcagctaaat
    22261 tctgatcata gaatttgagc aagagtccca ttaccactaa ccccaaacca taaaggtaag
    22321 gagtcagttt ccagatccat ttggaattca aatgcatgat gacaacgatt gctgccgctc
    22381 caactaaata ccagacaact tgcatcccta cgcctttcaa gactgctgga ttgttcggat
    22441 catggctaag tgctacatat aatgagagca gaccaatcaa acataacaga aagactggta
    22501 aaatcacgcc ataatcaata cgattatcgt tcgaagcttt taattttttt tccatgtttc
    22561 atcccttttc ttaaaaacca tactgctgat tattatagcg gtccagttag ataatggaaa
    22621 gtctttccat agacatttgg gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat
    22681 gcagtacaaa agcctactgc ttatctttag tgtatcatag aacttttttt gatacatgca
    22741 attatctacc agctgagatt ctcctcgctg tgagaagttt ctgtgctttc attgtatcga
    22801 aggatctctt cggcaatctc tagcagctga tcagaaacca gattggttcg ttggatgaaa
    22861 taaaaatatc gatggaaatt caacggagtc attggcacat tttctggaac agagcgctta
    22921 gacaaaatcg agcgaccgat tccttccttc agcaacgcaa caattacatc gttacttttc
    22981 acgatcatct tcttatcttg gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa
    23041 acaccagagt ttgcttctct tacaagccac aatttgcttt ttgggtcccc agcgatgact
    23101 agctggtctt ccatcaaaga gtatctttgt acgccggaag tctctaatgg cttttcgata
    23161 aaaccaaaat cggattcatg tttcgctact tgttctaatg attcatgaga attcgtgatt
    23221 tcgatctcaa aatgcacttt aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa
    23281 agatagacag caaaggtgtg ggaagcaacg atccgacata cttcttcttc attttgttca
    23341 ttgagcaatt tttgctgagt ttcttcccat tcttctaaca aaaccagtgt ccgttcatat
    23401 aataagtcgg cttgcttagt tggaagcatc tcttttcttc cgttacgttg aaaaagatcg
    23461 atattcaatt cccgctccaa ctgtttgatt tgattcgaga ctgctggttg agataaaaac
    23521 agtacttctg ctgctctgga aaaatttctt gtttcatata ctgctcgaaa agtcgttaat
    23581 aacttaaaca tggcaccact tccataactt tttatgataa ttactataaa aactatttga
    23641 tttaaatata caaaaaacca tctataatag aaatgtacaa caaaacagtt gctgatccaa
    23701 ctagtaactt agatgaagac aatcagctgt ctgcttgctc gtaatcatac attcctattc
    23761 attttacaat gtattcgttc atcttggtac taaaatctat taaataaaaa ggagaatatt
    23821 atatatgaag aactttttga aaattattcc gatcccgatc tgcgggttga tccttgggtt
    23881 agcttccctt ggaaatctgt ttaaagacta tcaccatgtt gctttaggca atctaactgg
    23941 cggtatctct atgattctta tgatattcat ttttgttaaa ttgtttatct tattcgaaca
    24001 cacgaaacaa acgttgcatg atccaatcat cgcctccgtt tcaccaactt ttacgatgtc
    24061 actcatggtt atttgtactt attttgtttc atatgaaagt attgcaccaa ttgtcaaatt
    24121 catttggctg tgcgctgtta tcttccaagt gatccttgtc ttttatttca attatcacca
    24181 tgttatcaaa gctgatcttt caattgaagc aatttatcca agctggttta tcatttacgt
    24241 tggatttggg gtcatcactg taactgccgg gaacttcttc ccacttattg gaaaaatttt
    24301 cttttggatt tcattgatct gctatgcgtt cttattgccg atcatcatca aacgtgtctt
    24361 tttcgtaaga aatatggctc atccgacacg tccgctgatt acgatcgttg cagcacctgg
    24421 ttctctttgc ctaacaggtt atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt
    24481 agcattgttc gctttatctc aagtattgta tttcgttgtg ctgtctatgc ttccacgttt
    24541 gttcaagctt cgtttttatc caagctatgc agcctttaca ttcccgcttg ttatctctgc
    24601 aacagctata tttactactg ttcattactt caacagttta ggtatctata gtagcgtatt
    24661 ggatttctta aaagttgcgg aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc
    24721 tcattacgta cgtttcttaa ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa
    24781 aatcgcttaa taatcgattc aataggttgt gaaaaagctg ttctgcatca agtaataaga
    24841 acggccaaac aaaaacagct gttcatgttt ttgtactggt tgggcttatt accgcagatg
    24901 caagcttttg aacaccgttt accaagaggt cgtaaaagaa gtggtcagct tcaagaaata
    24961 agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgtag
    25021 aagctacttc tggaacaccg tttattcgaa acaagaggtc gtacaagact tttgtcacga
    25081 cctcttgttc ttttgtcaaa atactggttt agtgctacta tgttcgagta tgcttttagt
    25141 atttattgac gaaaggaacc gatcaaatga aatcattgtc acatccaata aaatctgtaa
    25201 aggattcagc ggtccgcgaa atcttacccg gtttactgct ttcttttgcc atcgctgcct
    25261 tcagcaggct tcttgctatt tatctgccac agttaggtgg agcgactatt gctattttat
    25321 tgggtatttt attaggaaat acttttttta aacagcctgt tctagcaatc ggcacaaaat
    25381 ttgctgaaag caaactatta gaattctctg tttttttact aggtgcaact gtcacagctc
    25441 aaacgattgc tcaaattggt gtaaaaggaa ttgtttttgt gttactgcaa atgtcagtca
    25501 ctatcatcgg tgcttacttg attggtaaaa aactgcaatt cagcgatact tcttctttga
    25561 tgatggctgg aggaaacgct gtttgtggtt cttctgctat tgcttcaatc gccccttcca
    25621 tccatgcgaa agaggaagaa aaaggacaga tcatcacact agtcaatcta ctgggtactg
    25681 tgatgatgtt gactctccct tttcttggat tacagttgtt tggtggaaat ctcattgaaa
    25741 aaagtgcact tttgggtggt acactccaat ctgttggtca agtagtcgct ggggcaagct
    25801 tacttgacaa ttctgttgtc gaattcgcca tgctatttaa gattctacgt attatgatgc
    25861 tcgttgttgt tgtctatctg tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg
    25921 aaacgccaac tgatacgcag aaaaaacgaa aattaccgct tccttggtat gtattagggt
    25981 ttgtgatctt ttgtttggtc aacagcttgt tttctttacc tgtagttatt gaccaatcgg
    26041 cacattttat cagcagctgg tttgagatca ctgctttagc ggcaattggt ctgcgtttag
    26101 actttgcaaa attcatgaaa gaaggtacac gcttcttatc ttatggatta gctgtcgggc
    26161 ttctccaaac agtgatggct ttactattta tttggctgat caaagtttag aatgaacatg
    26221 aaaaaacagt tgtgattctc gcatggaaca atgtccgaat cacaactgtt tttaattttg
    26281 cagatatcct tctaacaaca acaattggcg tttgatttca accccatcta ttgcgtttcc
    26341 catatatcct gtcagttttc cattttgccc gatcactcga tgacaaggaa taacgattgg
    26401 caatggattg gttcgatttg cctgcccaat cgctcgcaca gctttcggac tatcaacagc
    26461 aacggctact tctttatacg ttcggatttc tccataagga atggtcgaca aagctttcca
    26521 gacattttgc tgaaaaggcg tacctctctt tatagataaa ggaacatcaa attcttttct
    26581 ttttcccgca aaatattctt caaactgttc ttgtgtgcgc gttaaaattc gctgattcgc
    26641 tgattcgctt tctgaaattg gctgaaaaga tacagcagtc aatccggatt catctgcatc
    26701 taaccataaa aagccgatag gtgtgtgtat tttcattttg tttgctccta gtcaatagac
    26761 tggaacttcc tcctttttct ctatccaaaa ctacttgcca actaataaaa aatgttctta
    26821 aaagtttgat aaaccgagga aacatggtaa actaaatacg ttagtttaca aaaagaatac
    26881 ctgttctttt acgtaaaatc aaattttaaa ggtgttattt aaggtttatt aaattttgaa
    26941 tgtaaaccat atataaacat acgattgaaa ggatagaaac gatgaaagta tcaattgttg
    27001 tcccatgttt taatgaagaa aaaactgttc ctcttttttt tgatgaggtt gaaaaattca
    27061 aaggtgccca tgagttcgaa tatttgttta ttgacgacgg atcaaaagac ggtacgttag
    27121 cagcaatcaa gaagctagcc tcagcttatc cttatgtccg ctttgtctca ttttccagaa
    27181 attttgggaa agaagcggct ctgtacgctg gattgcaagc ttctacagga gatttagtga
    27241 ccgtgatgga tgttgatctg caagatccgc ccgaactgct tcctgagatg atcaaccgga
    27301 tcactacttc tgatatcgat tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg
    27361 cgatacgcag tttctttgcg aaaaaattct accagttgat caataaaatc agcgatacag
    27421 aaatggtcga tggcgctcgt gatttccgct tgatgactcg tcaaatggtg gatgccgtcc
    27481 tagaattaac agaatacaac cgattttcaa aaggattatt tagctgggtc ggatttaaaa
    27541 cagaatatat ttcttatgaa aaccgcgaac gtgtggctgg cgaaacatct tggtccttct
    27601 ggaaattatt caactactcg attgatggta tcgtgaactt ctcggatgca ccgttaacga
    27661 ttgcttcatt tatcggcgct ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta
    27721 ttttgcgggc attgttgttt ggtgatccta catcaggatg gccttccatg gtctcgatct
    27781 tcctgttcat tggtggaatc caattgttat gtcttggaat catcgggaaa tatattggga
    27841 aaatcttctt agaaacgaaa cagcgtcctg tttatatcat aagagaaaca gaaaaagtga
    27901 agccaaatga attgaagcct caccaataat atacgtcaaa tgaagtaaac atttttttaa
    27961 atgaaaaagg actgtgacaa aagtcttgtc acagtccttt tttccgaata aatggtgttc
    28021 cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg
    28081 taaattcctt cttatttctt gaagctgacc acttctatca caacctcttt catatcttat
    28141 tacttcttca ttgctgcttt tagtgctgct gctaatgggc tttcttctgg ttcttctgct
    28201 tgtgcatatt ttttcagcaa gcgtcgttct tcatgtttgg tcattttctt cttctgctct
    28261 tttttatcaa gcaatttttc tgtcgttcca tcgtatttac aacggaaata agcaccgttt
    28321 ttaccttcga tgatttccat tttccggtga cattgcgggc atcgatgatt agagactttt
    28381 ggatcttttc ttctgcggta gttgcaatct gaattggtgc agacatagat tttgccgtca
    28441 cgtgtgtttt tctcgcgcag attggcccca cattctgggc atttcttttg ggtaatcgag
    28501 aaatcttgat attcttgttt gcttgatttg atctcattca ccaagcgttt tgtttctttt
    28561 tcgattccat tcaagaaggt tcctgcttgt ttctttcctg cagcaatagc ttcaaggtct
    28621 ttttcccagc tttctgtcaa atctggtgtt actaaagagg gatttaccaa tactaataat
    28681 tgcttacctt taggagatac ttgcagtcct tgaggggtac gttccatcag ttcagatttg
    28741 attagtttct cgataatctc tgcacgcgta gctggggttc ccagactgta tttttccatt
    28801 tgaccaagca agcttgcttc tgacaatggt ttaggcggtg cagatagttc ttttttgatg
    28861 gcgaaatcgg gattgattct cattcctttt tcccatttga ctgtttccgt ctcagtttca
    28921 gtttcgcctt tccatcctgc ttggacgaca cgattttgcc gaaaaatgaa tcgttcttta
    28981 ccaaattcaa ccgtaacttt tgtttggctg actttatggg gttgagcaaa cagtcctaaa
    29041 aaacgagaga caatcatttg atagattttt cgctcttcat tacttaattt ttcataacgt
    29101 gctctgtttt cagttggcaa aagtgcatgg tgatcggtca ctttactgtc ttggaatact
    29161 tttgtctgtt ggacgatggc tccgtttttc aaatactgct tgacctcagg agcaaagtcg
    29221 ctgactgctt gcaatcgttc tttcatcgta cttttcatgt catttgtcag atatttgctg
    29281 tctgtacgcg gataagtaac gatcttatgg gtctcataca aactttgtac aagacttaat
    29341 gttttctttg cagaatagcc atagcgttga ttggcttctc tttggatctc tgttaaatca
    29401 taaggcaatg gcgcagattc cgtttttaat ttttcttgga cttctgtgac agttcctttg
    29461 cttgtactga tcaattgaac aagtttttgt gcttcttctt gtgtttttaa agcaaattga
    29521 tttttttgaa gcattttggc ttgttctttt ccagcttgca acgtgatcgt gaaatacgtt
    29581 tgtggacgga actgctcgat tttcttttct tggtctctca ctaatgctag tgtcggtgtc
    29641 tgcacacggc ctgctgataa attatccgcg tacttcacag ttaaggctct ggtcacattc
    29701 aatcctacca gccagtccgc ttttgcccgg gcaagagcag attcatacaa tgcatcatat
    29761 tctttcgcag gtcttagttg cttgaatcca gttttgattg ctttatctgt ttgagaagag
    29821 atccataacc gcttcactgg tttattgaaa tgcacatact ctaagatcca gcgagcaaca
    29881 agttcccctt ctcttccggc atctgtcgca atgactgctt ctgagacatc ttttcgctga
    29941 gccagctgct tgattgcttt taattgatgt cctgttttag gaagtggttt gattcctagt
    30001 tttttaggga tcattggcaa cgtgtccatt tgccagctct gccattcctt attcagatct
    30061 tccggcattt tcaatcctaa taaatgacca agggcccaag taacgatcac cttgggtcct
    30121 tcataataat ttttgtgctt ttgattagca ccaagcacct tgcttaggtc tttcgcaacg
    30181 cttggttttt ccgctagaat tagttgtttc ataaaattcc tttctgcttt ctactgtgat
    30241 gggctgatga tttttcaaca acgcaagtcc cttgtattgc tgtaaatcat cgtcacactc
    30301 ttcacaccag cgctcatctc cgtcattcca aaaggcgctg agaaagttac tttttgcatt
    30361 gatatattca taattttgat ggatttcgtt ccattgtttt tcgtacgctt gtcgtgcttc
    30421 ttcgaaagac tcgaatgttt cttcttcttc aatatcttct tcccagtctt caaaaaacca
    30481 ccaaggctca ttgtccccga acatggtaat tacttgatac atactcgtgc tccttctttc
    30541 agcttttcta gactcatctt agtcactttt caagaaagtt gcaagaattt tctcttataa
    30601 aagggtcatt taatgaacat cttaaaattg ttacaaatga atctgattgt ttaaggcaaa
    30661 gtagcgataa agttattgat catccaaaaa tagctgtctt ctggatcgat tgttggttct
    30721 tttgtaaagt atcctaaaaa acgtaaggaa agataaccgt ggatcatact acgaagttcc
    30781 ctgcttcgat gaactttttc ttctttcgtt aattgaaaag cctcaaatat ctgcaaaatg
    30841 atctgattcg tttcatggat tccatctaat aaaacagctt cattggtatg cggtacactg
    30901 atcagtaact catacactgc ttgatttgaa aaagcaaagg atcgataagt ttctgcatag
    30961 atctgcagtg cttcctgacc actttttccc acaagagccc gtcgcagctg gtcattaagt
    31021 tcctgcagaa gtttcgctgt tagcgctgtt ttgacatcac gaatattttt gaaatggtta
    31081 tacagcgagg gagccttgat atccaagcgt ttggctaaat gctgaaaggt tatttgctga
    31141 acatccatct cttctgctag ttctcgaaag cagtcgatga ttttctcttg agacagattt
    31201 ctttttttca tttccatgtt tcctctcata aaataaatcc ttcactcttt cagtttacca
    31261 cgaaaaagaa agagaaaaaa gagactttca ttgataaact acatattgta gtttataata
    31321 aagagctgta gatgaaaatt ttcagaaaga aggagtctaa ttggctgcaa gaataaagga
    31381 aaaattattt aggaaaagag aaacttggga acaaaacttg tttgtcttat ggtttggaac
    31441 atttatggct ggtatcggat ttagtttagt catgcctttt atgtcacttt acatagatac
    31501 gttaggtaat tatagtacgt cacaattgaa cttttggagc ggattgactt tttcatctac
    31561 tttcttggtg actacgctta tctctccttg gtggggaaaa ttagccgacc aaaaaggaag
    31621 aaaattgatg ctgttgcgtg cttcattagg aatggcaatc gtcatttctt tgatgggttg
    31681 cgtgactagt gtgtatcaat tagtcgcatt gcgtctgctt caagggattt tttctggtta
    31741 tatcagtaat gccactgccc ttgtcgcaac tgggacgcca aaagaaaaaa gtggtcaagt
    31801 tcttggaacc ttagcaactg gttctgttac aggaacatta cttggaccgc ttcttggcgg
    31861 gatctctgct tcagcgtttg gctatcgccc tacctttttc attacaggga tcatcttatt
    31921 tttcgtcttc ttgttgagtc tgtttttcgt ccatgaaaaa tttgtcccag tgaaaaaaga
    31981 aaacatggtt tctgccaaac agattttcaa agacttgaaa tatccacacg tcgtcatcgg
    32041 tatgttcgtc acaacgatga tcatccaagc atccaacaat tccattagtc caatcatcag
    32101 tctgtacatt cgccaattgc tgcatggaca tgggaatgtt acgttgatca gcggaatcat
    32161 tgcttcgata cctgggattg ccactttgat agccgctcca agatttggtc gcttaggtga
    32221 caagattggg agtgaaagaa tacttgcgat tggtttgggt tttgcgattc tggtttatat
    32281 cccaatggca ttcgtcacga atgtttggca attagctgcc ttacgttttt tgattggtat
    32341 ctctgatgct tgtctgctcc ctgctgtcca agcattgatc acaaaatatt cgcctcatca
    32401 agctgctgga cggatcttca gttataacca atctttccaa gcaactggta acgtagtagg
    32461 gcctatgatc ggctccagtg tttccagtgc atttggttat cgtggcgtct ttctttcgac
    32521 ttctcttctt gttttggtca acttcttgct tgtacgacac aatacacaag agatccatca
    32581 gcaagaaact gcagaagaaa aagcttcagg agtctccata gcttcagggc actctccttc
    32641 tcatagacaa taagcgtaaa tgaaaaacac tgcttgaaca gttgtcacag actgtccttg
    32701 cagtgttttt gtttaggcct gttttttcaa gaactcagat aagaggaata atcccttttc
    32761 caaggtttct tttttacaac agtaacctag tcgggcatgc ttcggtaaat caaaggcact
    32821 tcctggtact aatagtactc ctgtttcttc taataaattg atacaaaatg tctggtcatc
    32881 ttcttcgatg gctaatttaa tgaaagaagt agagacgtaa ttgggagcga caagttcaac
    32941 tttcggttca ttggctaccc attgttttaa aatcacaagg ttctctgtaa tgatcttttt
    33001 gtttcgcgcc aatatttttt ctttattctt caatgcatgt acagcaagat catctgacag
    33061 gactccgccg caaatcatcg tgtagtctcg atactttcga aaaacttctg ccaactcagg
    33121 tccagtagct gtccagccaa tacgaatacc tggaatggag taggttttgg atagggaatt
    33181 ggttgctata cctttttcat agacatctac gatcgacaag aattcacctt tatccgttaa
    33241 aggcgcatat acttcatcta tcaatacata tgcatctact gtacgagcaa tttctgcgat
    33301 tttttctaaa gttttccgat ctagcaatgt gcctgtagga ttgtttgcac tattcaaaca
    33361 gatgattttt gtttcaggtt tgactaaggc ttctagctgt tctaaatcaa actgccaatc
    33421 ttcttcttcc ttcaaatgaa caaagtcaac agcggcacct aaagatttgg ggatgtcata
    33481 taattgctga taagtaggaa gcatcgaaac aacatggtct cctgcttcca ctaacgcgta
    33541 tagagctaaa agattagcac cagtcgctcc attcgtttgt aagatgtttt ccggatcgac
    33601 ggtttgataa agagaagcga cttcttgttt gaaaacatct gatccttcga tccagccata
    33661 gtccaatggt tcgtttttgc gtttttcgaa aaatttactt acgctagtcc catctaagcc
    33721 aacgagttcg tctaatgtca atgcttcgat cgagctttgt gcaatatcat ataccgcttt
    33781 cttttcccat ttatttagcc attcttctac accgaaattt gcgattttca tgtttctttc
    33841 ccccttatat cttgatccag cctacagcaa gcaacccgat tgaaagcagt gcggccacac
    33901 aaatcagccc aatcattact ttttcttttc ccgtaatttg atgtttggtt tcacgacaag
    33961 cgatccagta gaagcaaaaa ccaggaagat aggcaatact acataaaagg acataagaaa
    34021 atcctgctaa aatgatgccc atcaattcaa aaactacagc aatcccaccg atcactactt
    34081 gttttttgat tccttctttt aaagaaaact ggacttgata gatcccgaca agtagataac
    34141 aaatcaaaat tgccgctgta cataaagaat aggcaaattg ataggcataa tcagtaacta
    34201 agaaagtcaa taagaataga ttagtcaaga ttgcagtaac aactaacgaa taagttgggg
    34261 catttttttt attcgttttt ccccaacctt tcggcaacag accgtctcga gccatcaaca
    34321 tcgttgtttc tgctgggagc atcgtccaag acaaccaaga accaatgatc gaaataatca
    34381 agccgatatt gatcagcgct gctccccaat ttcccacgat tccttttaaa atatttgcca
    34441 tagcaggttg tgaaatagtt gctaactctt cttgagataa gacgccgtat ggcaagatcg
    34501 aagcaaggat atagataaac aataaacaaa gcagaccaat gatcgatgct tgctgggctt
    34561 ctgaacgttt cttcgcgcgg ttggctaaaa cacttgcgcc ttcgatccct acaaagaccc
    34621 acatcataac catcaaacaa cctttaatct gcaaccagat cgttccttgc tgccctgttc
    34681 cattcactag attatcagat acgttacccc aaaaatcagc agtgaatata ttcactttaa
    34741 atgcgataaa tacaaatatt aaaaatagga ataatggcac taatttacag atcgtcacga
    34801 ttgtattgat gacgctcgct ccttcaactc cattgtttac taataaagtc agaccccaaa
    34861 taaaaatact tgcaaataaa atagaaggaa cattttgtcc acctttgaat atcgggaaaa
    34921 attctcccat ggcgctcatc agcatcgttg caaaagcaac atttcccagc cacgcagaca
    34981 accagtaacc ccaaccactg atgaagccac ctaatttacc aaatgaagct tccgcgtaac
    35041 cgaaaatacc accatctaga tctggacgct tttctcctaa atgattcagc gataatacca
    35101 aagctaaaat accaatccca acgattatcc atgaaaggat cgctggccct ggagctgcag
    35161 aactagccag atcacttgtg atcccaaaca caccgcttcc gattgacgaa ctgacaacca
    35221 gagcaaccag tccccatttg ctaatacccg tcgatttctt ttccatttcc atcaccctca
    35281 cttataaaat tgaacaaatc acttgcaaaa gattcccttc tattttgcaa ctgctcttgc
    35341 ttaacaaaaa gcaactttat tcatattatt ggatattatg aactttttac acaagtattt
    35401 caagtataaa atgaataaaa attaatttta ttgcaaaaaa acagctatgc tatccaaaag
    35461 aaaaggtgtt tcttttgtta gccatagctg ttttgattca tttaatatcg cttaaacgta
    35521 ttgtaaaact tttgtttctt gatatgcgtg gtcgatcaaa ccaccaccca gacattccat
    35581 tccatcataa aatacaactg cttgtccagg agtgattgca cgtacaggtt cgtcgaatac
    35641 gacttctgca cgattatcat ccaataagcg aactgtcaca ggaacgtctt gctgacgata
    35701 acggaatttc gctgtacact tgaattcttt tggcattggt tcattcgtcg taaagtggat
    35761 ttcacttgca tccaagcttg tggcatacaa tgcaggatga tggaaccctt gccccacata
    35821 taatgtgttt gtcgctagat cttttcctac aacaaaccag ggttcttgcg aatctcctcc
    35881 gccaccgatc cctagtcctt gacgttgacc aatcgtataa tacatcaagc cagcatgttg
    35941 tcctttgact tcaccatcaa gagtgaccat atttcctttt tttgctggca gatagttgct
    36001 caaaaattgt ttgaagttct tttcaccaat aaagcagatt cccgttgaat cttttttctt
    36061 agcagtcgca aggcctgcgc gttcagcaat tgcccgtact tctgattttt ccatgccgcc
    36121 tagagggaac atcgtttttg aaagctgttc ttgtgataat tggctaagga agtaagtttg
    36181 atctttatta ttgtctactc cacgaagcat gtggaccacg ccgttttcat cgcgagtgac
    36241 ttgcgcataa tgtccagtag ctacataatc ggcgccaaga tccattgcat aatccaagaa
    36301 tgctttgaat ttgatttctt tgttgcacat cacgtctggg ttaggtgtac gtcctgcgcg
    36361 gtattcggct aggaaatatt caaatacacg atcccaatat tctttttcaa aattcacaga
    36421 gtaataagga atcccgattt gagcagctac tttcgctaca tccttataat cttccgtggc
    36481 cgtgcataca ccgttttcgt ccgtgtcatc ccaatttttc atgaatattc cgatcacgtc
    36541 gtatccttgt tcctttaaca aaagtgctgt tacagatgag tcgacaccgc cgctcatccc
    36601 tacgacaacg cgtgttttgc tgttgtctgt catgatatca ccatcatttc aattagattc
    36661 tgaaacatct acgagttgaa ggtcgcaact tttcagtatt ttacattata cgtcatattt
    36721 ctgaaaattt catcccttaa ttatcaagtc gtaaaaatac tgttctactg gacctattca
    36781 gtacacaaac atgatagtca accaaaaaat cactcatctt ttgagcattc gatataaaaa
    36841 attggcatac tcataaaaag ggccagcctt tcggcaataa ggaataattt tataaattgt
    36901 gaaaaactat ttttcaaaat tgcttcttat cgcttaaagc tgcacgtctc atttacaacc
    36961 ttttcttttt agatgttatg agttgctcag ataagtgatt ttgtttactc taagaaaaaa
    37021 gggaggcaaa agatacatga attactttat cagtgatatg cactttttcc atgaacgctt
    37081 attaggaaaa aacgactttg ctccgcggct ctttgcaaat gtggatgaaa tgaaccaaca
    37141 gttgatcact agctggaatg cagtaataaa agaaaccgac catgtttatc atctaggcga
    37201 ccttgctatg catccgcaat atgaaaaagc aaatgcagaa attttggaac tggtcactca
    37261 actgcaagga acaatccact tgatcaaagg gaaccatgac agtcgcgctt ttttccgtta
    37321 tttagaaaaa aatgatccta ggcttactga cggaagtccc aaattttatt tttatgatgt
    37381 aggtgccatc gttaaattcg accatcaaca atattacttg actcattatc cgatgttgct
    37441 cgggcctaac gacactatcc gcaatcttca tggacatatc catcattaca gcgtacctat
    37501 cgagaacgat gtcaatgtag gcgtagacgc accggaaaga gaactcttaa aggaaaaact
    37561 accatttggc actccgttat ctgagcaaca aatcaaagaa atctatgaag caaagaggaa
    37621 gtacgatagg tcgtgaaaaa actgccctat atcaagtatt aaacaaataa agataattac
    37681 tcatattttt catttgagta ggcttattgc cacaggaaca ttaggatcag aaatggagtt
    37741 gtaacaagac ttctgtcaca actccattcc atactattcg tctttttgct catggtgttc
    37801 ttttaccagt tttttctgta attctttatc ataatttcgg gcagctgacg ttaaaaataa
    37861 aacgattccg cataataaaa tcccgattcc ggaaaacaag aacatttttt ctacccctaa
    37921 acgatcagct aatggtcctg caaataataa tccaattgga cctgtcaggt tcatcaaaga
    37981 gttgaagttc cctaagacac gacctaaaac attcgattca taactctgct ggatcattgc
    38041 catcagcagt gtattgaaat atggcgttgc acaacctgct cctgcattca agataagaaa
    38101 gtacaagaac ccttgactat cgtttggaac caatccgctt gcaccgatcg tcaggcccat
    38161 aatgatgtag gctataataa taggtttcat tcggtctttc catgttccta taaaactgat
    38221 cagtgcaccg cctaagagca ttccgactgc gtagactact tccactgctc ccgcctgtcc
    38281 aacggaacca ccaaagtaat ctagcgtcat caatggatac agactgattg ctggcatgaa
    38341 caacagcatc acaaatgcac cattgatcgt tatgtaccat aaccctttat tatccatcaa
    38401 ttgctgcagc ccaaattttg catctttaaa aatagtaagc ggaacttcca atatttcagg
    38461 agaaactttt gggatcttca caaaaatcaa taagccgaca ccaaaaactg cccccaatac
    38521 atctaataaa ataaggtaat tgacaggaac gactgaaaac aaagccgcac ctagagctgg
    38581 cgcaatgatg aaattcgctg attggaccat tcctaactgt ccattcgtcc ttgttatgtg
    38641 agaagatgga acgattgtcg gcataatcga ctggatcgtt ggcatttgga atgtttgtgc
    38701 gaccgaacgc ataaacaaag acacaaaaac tagccaaatc gggaaggatt ctgaaatcgt
    38761 tccgactact gctaaaataa gcgcgaaaat cgctacaata atatccgtga caatcaaaag
    38821 agctttttta tcccagcgat ccactagcgg tcctacaaat gggcttagta aaaccatggg
    38881 aatcattcct agtaaagtag caaaactcaa aattgtcgcc gaaccggttt ctcttgtcaa
    38941 ataccagatg atcgcatatt ggacgaccat actagtgatt cctgacaaaa actgtcccga
    39001 taaaaagaac aaataatttc tcttccaatt cgttaaaacg accgactgtt cattttccat
    39061 aaacatccct cttttctttt tcattttgta tagcgtttgc acaacaatca ttatacggga
    39121 ttcttttttc aaaaaatacc tttttcccta aataacaatc gtttcaattt aaatttcttt
    39181 acttagaaac aataaatcag caaaatatta aaaataacaa catgtgttaa atttaaaaat
    39241 aaaaaagaca actaatttgc gtcgtcctta caaagttagt catctaacaa taggccgttt
    39301 caaaactagt tgtcttttta tatccacaga tcattcgact ttttcgaata acccacggct
    39361 aatcatccca tccatcagaa aataaaattt atcttggctc attttctgtt ttggatcttt
    39421 gaaaatattg actgctttca atccatttgc tgtagtgatc gacattttca atgtttgaat
    39481 atcttttgat acagtgattt ttaatttgaa tccttctttg ctttgcggcg tggcatctaa
    39541 tggtcggatc aattgataat tgcctccgtt tgtttctgta aatccattat cgcgcaatgt
    39601 atatcttttt gcatcaggat gcaaacgata gctaattggt gcgcccagta ccgttgctgt
    39661 tgtttcaaat gccatgattt tcacctcgat aaaattagtt tctctttatt ataaaagttt
    39721 atcctgattt ttactaggtt tttcagttaa gttccctaaa aaatgaaggc gttctctcag
    39781 acatctgttc atataagaat gacttcacta gttatttttt gacagcacga actaattgat
    39841 caaggaaata ctggatgtcg tcttcggtat ttcccaaacc aaagctgata cggatggatt
    39901 cttttagtat cggtgtatct tctccataca ttgcttccaa tacgtgtgaa ggctcgatat
    39961 ttcctgctgt acaagctgaa ccagtagaaa tagcgacccc attaagatca agcttcatca
    40021 gtagcaaatc atttgcttcg ccaattaagc gaagattcaa cacatgaggg agtttattcg
    40081 ttaaatcacc atttacttga taagggattt cgtttgcttc taatgtatca agaatcaatt
    40141 gctggaatcc tttatatttg ttgttacgtg cttcacgttc ttcaggtgac agcagttcaa
    40201 ctgcttttgc cataccccag atccctgcta aattttctgt acctgcacgc cgcttttctt
    40261 cctgctcccc gcctaaaagc aatgcaggga tatttactcc gtctcgttta tataagaaac
    40321 cgacgccttt tggcccattg attttatgag cagagatact taataaatcg attcctaatt
    40381 cttctggatg gatcgtttgg tttccgtacg cttgtaccgc atctgtatgg aaataagctg
    40441 gatgatccgc taaaagttca ccgatttctt tgattggcat caaatttccg atttcattgt
    40501 tcccgtacat cacagagacc aagatcgtat cttctcgtaa cgcttttttg acctcttcga
    40561 tcgacagatt ccctttttca tctacaggca gatatgtaac gtcaaacccc tttgtctcta
    40621 aatatttcat ggtattaaga acagcaggat gttcgatagc tgtagtgatt agatgtctgc
    40681 cttctttttg acgcgagaac gctgtttcta agatagctgt attatcccct tctgttcccc
    40741 cactgttaaa gatgatctcg tgaggttttg cttgcagact gtctgcaatg atttgtcttg
    40801 ctgtttccaa tttttcatgc gcgtggcgac caaatccatg tatgcttgaa ggatttccaa
    40861 acgtgtcttg catgattgcc gtcatcgctt caattacttg tggatgcatg ggggtagttg
    40921 ccgcatgatc cagataaata gttttcactt cattcatccc ttccaaatta ttgctaggca
    40981 cattctagca taaaatagcg aacaaaccaa agaagagctc atttttgtct agaggacgtt
    41041 tcatgggcat agaaaaaagg agctgtgaca aaagtcttgt cgcagctcct tgaagctgct
    41101 cacttctgtc acaacttctt
//
LOCUS       DAZKBH010000012        40414 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000012 DAZKBH010000000
VERSION     DAZKBH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.012"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACG8OO_000659"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACG8OO_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9044590.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACG8OO_000660"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACG8OO_000660"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9044591.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="ACG8OO_000661"
     CDS             complement(2303..2965)
                     /locus_tag="ACG8OO_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9044592.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="ACG8OO_000662"
     CDS             3149..4795
                     /locus_tag="ACG8OO_000662"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9044593.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="ACG8OO_000663"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="ACG8OO_000663"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9044594.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="ACG8OO_000664"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="ACG8OO_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="ACG8OO_000665"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="ACG8OO_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="ACG8OO_000666"
     CDS             complement(8944..10023)
                     /locus_tag="ACG8OO_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9044595.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="ACG8OO_000667"
     CDS             complement(10126..10566)
                     /locus_tag="ACG8OO_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9044596.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="ACG8OO_000668"
     CDS             complement(10623..11033)
                     /locus_tag="ACG8OO_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9044597.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="ACG8OO_000669"
     CDS             complement(11213..11647)
                     /locus_tag="ACG8OO_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9044598.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="ACG8OO_000670"
     CDS             complement(11747..12595)
                     /locus_tag="ACG8OO_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9044599.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="ACG8OO_000671"
     CDS             12984..14750
                     /locus_tag="ACG8OO_000671"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFP9044600.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="ACG8OO_000672"
     CDS             14852..16111
                     /locus_tag="ACG8OO_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9044601.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="ACG8OO_000673"
     CDS             16258..17559
                     /locus_tag="ACG8OO_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9044602.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="ACG8OO_000674"
     CDS             17562..18413
                     /locus_tag="ACG8OO_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9044603.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="ACG8OO_000675"
     CDS             18426..19241
                     /locus_tag="ACG8OO_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFP9044604.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19392..20558
                     /locus_tag="ACG8OO_000676"
     CDS             19392..20558
                     /locus_tag="ACG8OO_000676"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9044605.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20788..21435
                     /locus_tag="ACG8OO_000677"
     CDS             20788..21435
                     /locus_tag="ACG8OO_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9044606.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21519..22463
                     /locus_tag="ACG8OO_000678"
     CDS             21519..22463
                     /locus_tag="ACG8OO_000678"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFP9044607.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22598..24049
                     /locus_tag="ACG8OO_000679"
     CDS             22598..24049
                     /locus_tag="ACG8OO_000679"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFP9044608.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24070..24906
                     /locus_tag="ACG8OO_000680"
     CDS             24070..24906
                     /locus_tag="ACG8OO_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9044609.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24906..25565
                     /locus_tag="ACG8OO_000681"
     CDS             24906..25565
                     /locus_tag="ACG8OO_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9044610.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25691..26380
                     /locus_tag="ACG8OO_000682"
     CDS             25691..26380
                     /locus_tag="ACG8OO_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044611.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26386..27342
                     /locus_tag="ACG8OO_000683"
     CDS             26386..27342
                     /locus_tag="ACG8OO_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFP9044612.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27386..27898
                     /locus_tag="ACG8OO_000684"
     CDS             27386..27898
                     /locus_tag="ACG8OO_000684"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9044613.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28154..28702)
                     /locus_tag="ACG8OO_000685"
     CDS             complement(28154..28702)
                     /locus_tag="ACG8OO_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9044614.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28791..29972)
                     /locus_tag="ACG8OO_000686"
     CDS             complement(28791..29972)
                     /locus_tag="ACG8OO_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFP9044615.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30208..31716)
                     /locus_tag="ACG8OO_000687"
     CDS             complement(30208..31716)
                     /locus_tag="ACG8OO_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFP9044616.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31875..32066
                     /locus_tag="ACG8OO_000688"
     CDS             31875..32066
                     /locus_tag="ACG8OO_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044617.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32269..33186
                     /locus_tag="ACG8OO_000689"
     CDS             32269..33186
                     /locus_tag="ACG8OO_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFP9044618.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33179..34252
                     /locus_tag="ACG8OO_000690"
     CDS             33179..34252
                     /locus_tag="ACG8OO_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044619.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34400..34918
                     /locus_tag="ACG8OO_000691"
     CDS             34400..34918
                     /locus_tag="ACG8OO_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044620.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34899..35291
                     /locus_tag="ACG8OO_000692"
     CDS             34899..35291
                     /locus_tag="ACG8OO_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFP9044621.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35367..36302
                     /locus_tag="ACG8OO_000693"
     CDS             35367..36302
                     /locus_tag="ACG8OO_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9044622.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36435..37214
                     /locus_tag="ACG8OO_000694"
     CDS             36435..37214
                     /locus_tag="ACG8OO_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9044623.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37207..37524
                     /locus_tag="ACG8OO_000695"
     CDS             37207..37524
                     /locus_tag="ACG8OO_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9044624.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37768..39378
                     /locus_tag="ACG8OO_000696"
     CDS             37768..39378
                     /locus_tag="ACG8OO_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9044625.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39535..40383
                     /locus_tag="ACG8OO_000697"
     CDS             39535..40383
                     /locus_tag="ACG8OO_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9044626.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13231 a         7260 c         7799 g        12124 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    19321 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    19381 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    19441 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    19501 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    19561 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    19621 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    19681 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    19741 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    19801 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    19861 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    19921 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    19981 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    20041 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    20101 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    20161 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    20221 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    20281 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    20341 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    20401 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    20461 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    20521 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    20581 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    20641 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    20701 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    20761 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    20821 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    20881 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    20941 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    21001 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    21061 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    21121 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    21181 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    21241 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    21301 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    21361 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    21421 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    21481 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    21541 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    21601 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    21661 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    21721 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    21781 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    21841 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    21901 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    21961 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    22021 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    22081 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    22141 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    22201 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    22261 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    22321 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    22381 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    22441 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    22501 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    22561 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    22621 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    22681 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    22741 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    22801 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    22861 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    22921 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    22981 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    23041 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    23101 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    23161 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    23221 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    23281 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    23341 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    23401 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    23461 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    23521 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    23581 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    23641 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    23701 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    23761 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    23821 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    23881 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    23941 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    24001 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    24061 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    24121 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    24181 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    24241 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    24301 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    24361 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    24421 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    24481 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    24541 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    24601 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    24661 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    24721 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    24781 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    24841 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    24901 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    24961 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    25021 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    25081 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    25141 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    25201 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    25261 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    25321 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    25381 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    25441 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    25501 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    25561 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    25621 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    25681 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    25741 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    25801 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    25861 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    25921 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    25981 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    26041 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    26101 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    26161 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    26221 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    26281 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    26341 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    26401 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    26461 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    26521 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    26581 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    26641 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    26701 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    26761 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    26821 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    26881 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    26941 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    27001 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    27061 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    27121 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    27181 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    27241 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    27301 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    27361 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    27421 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    27481 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    27541 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    27601 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    27661 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    27721 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    27781 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    27841 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    27901 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    27961 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    28021 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    28081 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    28141 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    28201 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    28261 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    28321 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    28381 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    28441 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    28501 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    28561 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    28621 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    28681 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    28741 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    28801 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    28861 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    28921 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    28981 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    29041 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    29101 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    29161 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    29221 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    29281 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    29341 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    29401 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    29461 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    29521 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    29581 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    29641 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    29701 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    29761 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    29821 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    29881 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    29941 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    30001 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    30061 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    30121 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    30181 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    30241 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    30301 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    30361 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    30421 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    30481 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    30541 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    30601 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    30661 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    30721 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    30781 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    30841 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    30901 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    30961 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    31021 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    31081 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    31141 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
    31201 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    31261 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    31321 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    31381 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    31441 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    31501 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    31561 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
    31621 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    31681 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    31741 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    31801 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    31861 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    31921 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    31981 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    32041 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    32101 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    32161 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    32221 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    32281 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
    32341 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    32401 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    32461 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    32521 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    32581 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    32641 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    32701 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    32761 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    32821 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    32881 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    32941 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    33001 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    33061 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    33121 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    33181 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    33241 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    33301 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
    33361 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    33421 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    33481 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    33541 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    33601 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    33661 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    33721 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    33781 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    33841 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    33901 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    33961 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    34021 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    34081 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    34141 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    34201 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    34261 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    34321 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    34381 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    34441 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    34501 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    34561 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    34621 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    34681 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    34741 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    34801 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    34861 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    34921 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    34981 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    35041 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
    35101 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    35161 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    35221 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    35281 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    35341 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    35401 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    35461 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    35521 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    35581 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    35641 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    35701 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    35761 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    35821 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    35881 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    35941 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    36001 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    36061 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    36121 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    36181 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    36241 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    36301 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    36361 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    36421 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    36481 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    36541 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    36601 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    36661 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    36721 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    36781 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    36841 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    36901 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    36961 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    37021 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    37081 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    37141 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    37201 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    37261 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    37321 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    37381 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    37441 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    37501 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    37561 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    37621 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    37681 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    37741 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    37801 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    37861 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    37921 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    37981 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    38041 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    38101 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    38161 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    38221 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    38281 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    38341 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    38401 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    38461 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    38521 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    38581 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    38641 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    38701 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    38761 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    38821 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    38881 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    38941 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    39001 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    39061 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    39121 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    39181 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    39241 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    39301 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    39361 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    39421 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    39481 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    39541 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    39601 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    39661 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    39721 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    39781 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    39841 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    39901 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    39961 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    40021 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    40081 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    40141 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    40201 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    40261 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    40321 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    40381 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DAZKBH010000013        39929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000013 DAZKBH010000000
VERSION     DAZKBH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.013"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1235
                     /locus_tag="ACG8OO_000698"
     CDS             <1..1235
                     /locus_tag="ACG8OO_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9044627.1"
                     /translation="RYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLT
                     ECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWE
                     LPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEI
                     KKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVF
                     IDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQ
                     TNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVH
                     YLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENII
                     PISINTIDRAIISAVQNK"
     gene            complement(1327..1692)
                     /locus_tag="ACG8OO_000699"
     CDS             complement(1327..1692)
                     /locus_tag="ACG8OO_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9044628.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1689..2054)
                     /locus_tag="ACG8OO_000700"
     CDS             complement(1689..2054)
                     /locus_tag="ACG8OO_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9044629.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2246..2494)
                     /locus_tag="ACG8OO_000701"
     CDS             complement(2246..2494)
                     /locus_tag="ACG8OO_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044630.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2493..2621
                     /locus_tag="ACG8OO_000702"
     CDS             2493..2621
                     /locus_tag="ACG8OO_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFP9044631.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2678..4372
                     /locus_tag="ACG8OO_000703"
     CDS             2678..4372
                     /locus_tag="ACG8OO_000703"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFP9044632.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4439..4645
                     /locus_tag="ACG8OO_000704"
     CDS             4439..4645
                     /locus_tag="ACG8OO_000704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044633.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5023..6156
                     /locus_tag="ACG8OO_000705"
     CDS             5023..6156
                     /locus_tag="ACG8OO_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP9044634.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6187..6507
                     /locus_tag="ACG8OO_000706"
     CDS             6187..6507
                     /locus_tag="ACG8OO_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFP9044635.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6536..7000)
                     /locus_tag="ACG8OO_000707"
     CDS             complement(6536..7000)
                     /locus_tag="ACG8OO_000707"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9044636.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7132..8385)
                     /locus_tag="ACG8OO_000708"
     CDS             complement(7132..8385)
                     /locus_tag="ACG8OO_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9044637.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8414..8731)
                     /locus_tag="ACG8OO_000709"
     CDS             complement(8414..8731)
                     /locus_tag="ACG8OO_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9044638.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8757..9077)
                     /locus_tag="ACG8OO_000710"
     CDS             complement(8757..9077)
                     /locus_tag="ACG8OO_000710"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9044639.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9541..10956)
                     /locus_tag="ACG8OO_000711"
     CDS             complement(9541..10956)
                     /locus_tag="ACG8OO_000711"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9044640.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(10973..12289)
                     /locus_tag="ACG8OO_000712"
     CDS             complement(10973..12289)
                     /locus_tag="ACG8OO_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9044641.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12439..13137)
                     /locus_tag="ACG8OO_000713"
     CDS             complement(12439..13137)
                     /locus_tag="ACG8OO_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9044642.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13426..14415)
                     /locus_tag="ACG8OO_000714"
     CDS             complement(13426..14415)
                     /locus_tag="ACG8OO_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9044643.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14495..15328)
                     /gene="msrA"
                     /locus_tag="ACG8OO_000715"
     CDS             complement(14495..15328)
                     /gene="msrA"
                     /locus_tag="ACG8OO_000715"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9044644.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15503..17137)
                     /locus_tag="ACG8OO_000716"
     CDS             complement(15503..17137)
                     /locus_tag="ACG8OO_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFP9044645.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17124..18812)
                     /locus_tag="ACG8OO_000717"
     CDS             complement(17124..18812)
                     /locus_tag="ACG8OO_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044646.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            18958..20595
                     /locus_tag="ACG8OO_000718"
     CDS             18958..20595
                     /locus_tag="ACG8OO_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044647.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(20740..21480)
                     /gene="truA"
                     /locus_tag="ACG8OO_000719"
     CDS             complement(20740..21480)
                     /gene="truA"
                     /locus_tag="ACG8OO_000719"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9044648.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(21671..22306)
                     /locus_tag="ACG8OO_000720"
     CDS             complement(21671..22306)
                     /locus_tag="ACG8OO_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP9044649.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(22453..23673)
                     /gene="sstT"
                     /locus_tag="ACG8OO_000721"
     CDS             complement(22453..23673)
                     /gene="sstT"
                     /locus_tag="ACG8OO_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9044650.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            23892..24890
                     /locus_tag="ACG8OO_000722"
     CDS             23892..24890
                     /locus_tag="ACG8OO_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044651.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            25086..26921
                     /locus_tag="ACG8OO_000723"
     CDS             25086..26921
                     /locus_tag="ACG8OO_000723"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9044652.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(26981..27697)
                     /locus_tag="ACG8OO_000724"
     CDS             complement(26981..27697)
                     /locus_tag="ACG8OO_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFP9044653.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(27924..28466)
                     /locus_tag="ACG8OO_000725"
     CDS             complement(27924..28466)
                     /locus_tag="ACG8OO_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9044654.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(28471..28698)
                     /locus_tag="ACG8OO_000726"
     CDS             complement(28471..28698)
                     /locus_tag="ACG8OO_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFP9044655.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(28777..30657)
                     /locus_tag="ACG8OO_000727"
     CDS             complement(28777..30657)
                     /locus_tag="ACG8OO_000727"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9044656.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(30846..31460)
                     /locus_tag="ACG8OO_000728"
     CDS             complement(30846..31460)
                     /locus_tag="ACG8OO_000728"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9044657.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            31602..32162
                     /locus_tag="ACG8OO_000729"
     CDS             31602..32162
                     /locus_tag="ACG8OO_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9044658.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(32467..32781)
                     /locus_tag="ACG8OO_000730"
                     /pseudo
     CDS             complement(32467..32781)
                     /locus_tag="ACG8OO_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(33035..34670)
                     /locus_tag="ACG8OO_000731"
                     /pseudo
     CDS             complement(33035..34670)
                     /locus_tag="ACG8OO_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35109..35978)
                     /gene="scrK"
                     /locus_tag="ACG8OO_000732"
     CDS             complement(35109..35978)
                     /gene="scrK"
                     /locus_tag="ACG8OO_000732"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFP9044659.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(35998..37119)
                     /locus_tag="ACG8OO_000733"
     CDS             complement(35998..37119)
                     /locus_tag="ACG8OO_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFP9044660.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(37121..37516)
                     /locus_tag="ACG8OO_000734"
     CDS             complement(37121..37516)
                     /locus_tag="ACG8OO_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9044661.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(37535..38332)
                     /locus_tag="ACG8OO_000735"
     CDS             complement(37535..38332)
                     /locus_tag="ACG8OO_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9044662.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(38334..39122)
                     /locus_tag="ACG8OO_000736"
     CDS             complement(38334..39122)
                     /locus_tag="ACG8OO_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9044663.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(39136..39612)
                     /locus_tag="ACG8OO_000737"
     CDS             complement(39136..39612)
                     /locus_tag="ACG8OO_000737"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9044664.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(39684..>39929)
                     /locus_tag="ACG8OO_000738"
     CDS             complement(39684..>39929)
                     /locus_tag="ACG8OO_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9044665.1"
                     /translation="DLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEF
                     QKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT        12618 a         7873 c         6393 g        13045 t
ORIGIN      
        1 accgttacac tttatatttt tcagaacata tacctcgatg caagatcatg cagcaattgt
       61 ctcaaaattc gttgttttta aaaacttgtc tgctttattt ggaagaagat gaacctgatt
      121 atcttcaatt aactgaatgt gaattcatct ctgtcagcaa agcttattca ttaaaaaaac
      181 aagtattagc ttactttaat gactgcggga tcgaaatcga ccgctactcc cctcgattca
      241 ctgaaatgga aagacgtctg ctgcttctta atgtgtcgta tcgtttaggc ggttttaatt
      301 cttgggagct acctgaatcg ttttttgaac gtgccgatcg tttcattgaa tccgtaacgg
      361 aaaatagtgg cagattttat gataaagaaa ataaagaaat cttatcgatt ggttttgcta
      421 tcagtttcct acgccagcaa gtgtgtgctg tgacaataga ttcaaaattt atcgaagaaa
      481 tcaaaaaacg tcctatttac aattatgtcg aatcagcatg ggaaaacact gatttccaaa
      541 cctattataa aaaagaagaa ttcgccttta ttttgacact attcaatctt tgtaattatg
      601 gtttccattc ttatcagttg attgcggaag actttcaaca gctacaccaa gtgtttattg
      661 acaatactcc cgaaataaaa gaactagttg ctacctttga atctcacttc aatcaagaat
      721 tattcggaaa ccaaccgttc gaacgtgcgc tgattcatct gatgcgttcg gcttgggata
      781 actatcaatt attcatgcca gaaaaatttt atcttttaaa tgaggaacag acaaatttat
      841 ataaagaagt ccagacaatt ttttccagct ggtccagcca gttaccttat gatctacggt
      901 tgaatcctaa ttgcatgcgg gcttttgtga tcgaattgag cggaattctc cgtctgacaa
      961 aagaacatct gactatctat atcgtcacaa attcggatgt tcattatctg atttatcggg
     1021 aagccttgga agctgtaact actttcgatt ttcaagttgc accaacaatt tattcaagta
     1081 tatctgatat aaagaagtat gcgcagcagt cctcaaaccg ggtattatgt gaacgtacat
     1141 tatatacacc agatgcggtc caatatgaaa atattattcc tatttcgatc aatacgatcg
     1201 atcgagctat catttcagct gtgcaaaaca aataaggggc tgtgacaaaa atcatgtcac
     1261 agcctcttat ttgttgaaga tgcccatttc tgtaacaatc tcttgataca cgtcaggctc
     1321 acaacctcac ttattttgga catctagggg tccgatctct ttatttaaaa aataagaaat
     1381 gatggttcgg ataactacca acgccgctag ttggaaaata tcctgcaagg taggtttgat
     1441 aatcgattcg atgatcccag ctgaaacaag aatttccaaa cttagaagta tatagcctcc
     1501 caacatcttt ttaatagcat tgttctcgct tactcggtcg ctttgcactt ttgtatggac
     1561 aaataaatat ttcaaaaaat ccttgattga tagacaaaca ccccaaacca ataaacagat
     1621 agcaaagata tcaagtccca gtacaagttt ttcgaaatat ggaagaagaa actgcatcaa
     1681 ctctgtcgtc atcctacctc tcctcttctt tgtttttctt tgtcttctat ttcctgattc
     1741 aagaaataag aaatgactgt tcgaatgata ataataagtg caagcttcaa aatatctgtc
     1801 caagtcggct tgataactga ctcgatgata tcagcaacga ctagtacttc caagcttaac
     1861 aaaatataac ttgctagcag ttttcttgta tctgcattac gagtattacg ttctgcatag
     1921 tttttcttga catgagacca atcaaaaagt ttgaatagaa caagaacgat cccgacaatt
     1981 aagataccga tagaacaaat atttaaaata gcaatgatcc attctagaaa ttcaattaaa
     2041 tttgagcgaa acatatacgc tacctacttt cttttaattt gttcctattc ttttattgta
     2101 aaagaaagtt accttgttcg tctatttaaa agttgttcta atgataacgg ttcgcttaaa
     2161 tttttctttt tcactctccc atttttctcc ctccatccat aaatcgtgga agtatccaaa
     2221 acaacgtagc aagttaaaat aaggcttagt ccgtcatcag cttttcaaag aagcactgtc
     2281 tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc atacacctcc tcaaatcttt
     2341 tcttcttacc taccctattt ttatttacag gaaaatcaga cacttcttct tttttgtcaa
     2401 aatttccttt ttcttctcgt cctatcattt ggcattctaa taaaaacgaa ctatccttgg
     2461 atatagaaag agaaaacaag gaggcgtttt tcatgagtaa aaaattaatc aaagtcggca
     2521 tcggtttagg tttgcttgcc cttggcgctg catatctagg aaaaaagaca ggactattcg
     2581 aagatgacag tcatttatat gatgaattcg aatcaatcta gcatcctatt tagttgaatc
     2641 actacggaca tacttggaag acattggagg aatcacaatg agaaaaagta aagcaataat
     2701 gattggtgcc ggtattgcca atatggcagc ggctgtttat ttgattcaag aaggcaaatg
     2761 gaaaggggat cagatcacct tttattcatt ggatgatcac ggttcaaatg atggtgcacc
     2821 gaccgacact gtagtggatg aatattggaa taaaaaccat ccattagaaa accaaaaagg
     2881 ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc tatgtagatt tgatggatct
     2941 gttaagtcga ataccttctg ttactgagcc agggatgact gctgaagaag atacgagaga
     3001 ttttgatgca aaacaccgaa cctttgacaa agcacgtttg atggaaggtg gcaaaggaat
     3061 cattgatggc ggaaaactgg gattcaataa taaagaccgt attcttctga ctaaactgat
     3121 tgctatgcct gattcagaag aagaaaaact agacaatatc actatcgaag aatatttcaa
     3181 agatgatcca cacttttttg aaacaaactt ctggtttatg tgggaaacaa catttgcttt
     3241 cagaacacgt agttctgctc aagaattgcg tcgctatatg catcaaatga tttatgaatt
     3301 cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt tacaatcaat acgaaagtat
     3361 tatgcttcct ttgatcgagt acttgaaaga acaaggatgc aaaatcattt tgaaccgccg
     3421 tgtcactgac tgggaattca aagaaacacc gatgcaagat gaaatcactg tgactggttt
     3481 gaaaattacc aatacactga ctcaagaaga agaatacgtt gtggtcgatg aagatacagc
     3541 agttattttc actaatgggt ccattacaga ttcagctact ttaggagatt acgaaacacc
     3601 agctgtagaa aatatggatt atggtgcagc ttctagtctc tggaaaaaag ccagtgaacg
     3661 tttctacaac ttgggaaatc ctgacaaatt ctttgctgat cgagatgcaa gcgaatgggt
     3721 cagctttacc ctgacgacga aaaaccattt gttgttgaat gaaatcaccc gtatcacgac
     3781 tcaagaacca ggaaatgcct tgaattcatt tatttcaacc acaccgatca cgccgttagg
     3841 ccaaaaagat gtcaatatgt ctattgtcgt gcatcatcaa ccgcatttta cgtctcagaa
     3901 accaaatgaa acagttattt ggggttattt cctttatcca agacgacgtg gcgaatttgt
     3961 ggataaacag tatattaaaa tgaatggaaa agaaatgcta gaagaactga ttggacaact
     4021 ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa gaaacagaaa ttttcgacag
     4081 cgtaataaat aatatccctg tctatatgcc ttatgcttcc gcgttgttca ataatcgtgc
     4141 caaaagcgat cgaccaaaag tcattcctaa acattcgacc aatctggcct ttacaggaga
     4201 atttgtcgag cagccttatc agatgatctt tactgaacaa agtgctgtac gttctggaga
     4261 aatcgctgct ttccattttg cgggtattcc tatgtcccgt cttgtcaaaa caccgcgtta
     4321 cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa atgtttgaat aatcaagagg
     4381 ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc aggctaaaac agcttcttat
     4441 gtttttgatt tggttggact tattaccgca gatgcaagct tttgaacacc gtttaccaag
     4501 aggttatgaa aaagctgccc tgcatcaagt aataagagca gccaagctaa aacagcttct
     4561 tatgtttttg acttggttag acttattacc gcagatgcaa gcttttgaac accgtttacc
     4621 aagaggttat gaaaaaggcg tttagtttcg agtatgaaga aaaattttgt gaaaatggtt
     4681 cctcacgttt ttgcataaat ttgacttaat accgagaaac tgatcccttc tgtcataacc
     4741 tctttttatt tatgtctgtt tctttttgaa aacaactatt ttttcaacgt ttactctctt
     4801 gccaatccaa catcatctaa cggatacaac tatttcaatg attaccatta cactaaaaaa
     4861 tcggaaacac acctctccga acacaaatac ataagactat agaatgtttc atttaagaaa
     4921 aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa atgaaattat ctgtagtact
     4981 aataatgcag acaattttat gaatctgaaa ggagcatctc caatgaaagc agtcacttgg
     5041 caaggaaaac agaaaatgga aatcagaaac gtagatgatc caaaaatatt agaaccaaca
     5101 gatgcaatca tccgtatcac agcaacagcc atttgcggct ccgatctgca tctttaccat
     5161 catggtgaag cagttttgga aaaagggtat gttgtaggac acgaaccaat gggaatcgtt
     5221 gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc aacgtgtggt tatccctttt
     5281 aatattagtt gtggtcattg tcatttctgc ctgaatcaca tggaaagcca atgtgatgaa
     5341 tcaaatccag atcagttata tggcgggctt ttcggatttg gcaagttgaa cggtaatcat
     5401 tggggtggtc aagcggaata tctaagagtt ccttatgcag actctacttc atttgttgta
     5461 ccagataatg atcttcctga tgaaaaagtt ttatttctat cagatatttt gcccacagct
     5521 tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata cagttgtgat tctaggttct
     5581 ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg ctggagcaga gcgcgtaatc
     5641 gcagtagatc cagtacagca ccgtctagat aaagcggcta attacaatgg ggttgaaact
     5701 tatttcttag aagacacggc acaagctgga acagaattgt atgaactggc taaaggtggc
     5761 gcagatgtca ttatcgattg tgtaggaatg gacggattag agcctgtcaa agaaaaagcg
     5821 aaaaatttag taagcttaca aagcggcaca atctctccat tacagatggc atcgcaagct
     5881 gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata tgactccagc ttcttcttac
     5941 ccgctgcaag aattttttat gcgggatatc gctgtcaagc atggacaggc accaattatc
     6001 catctaatgc caaaaattta cgcaatgatt gcagaaggac tattcgatcc tacacaaatc
     6061 attactcatt cgctgccact atcagaagca gcacaagcat atgagatctt tgacaaaaaa
     6121 gaagataaaa atataaaagt tgttttgaaa ccttgaattc agtaaaaaat acaggtgatt
     6181 ttctttatgc ccaaaatcaa acgaaatacc catgagatcg aaataggaaa agaagaagat
     6241 atcgaaatac gaggacgtcg cttccgcttc tatttccaaa atcgttacac attgatctca
     6301 ctggctgtcg atttattaac cgggattttc tatatttttg gaagccttgc ttccttaact
     6361 aaaatccccg atcgatatgg cacctatttt tatttggctg gtgctatttt cttgacgatt
     6421 cgtcctattc tcagaatcgt aaggaacatt tttatttacg acgaaaaaaa agcaatgaag
     6481 agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat ctcatgcctc ttttttcacc
     6541 ctatcgtatc actttgatac agacgagcgg tcgtcccact ttcttccttg accattgtca
     6601 aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc tgtaattaag taaagttttt
     6661 cgtacccaag ttgatgcatt tcttttctga tttccgctaa tatccgcttg gctatcccct
     6721 gcttacgctt ttccggttca acaaacaggg cacaaagatt tggtgtcaga tcagggcggt
     6781 tatggaagtc gttttcaatg acgcccgctc ctccgacgat ggtttctttt tcatctaaga
     6841 ccacatacca ttgaggaata ctttccttat tagcgattcc agtacgaata ctttcctcat
     6901 acacaacagc aggaactccc cattttgatg aaaaccaatt cgcgctttgt tttactaatt
     6961 ctgggtgttc tcttaatttg atccagttat atgtaaccaa tgtttaaacc tccgactaaa
     7021 ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat tagcaatgac aacaatagtg
     7081 atgtaaaaaa aaaaacggaa aacaatcact tgttctccgt tttttgatta atcaagcatc
     7141 taaagcttct tttcttcttt cttggatcgt agctaccttg acgaacggta gatagatcaa
     7201 aatagaaaga accaaattga cagcggctag tacaccacct gcaatacttt ttgttgccaa
     7261 tacaccgcca ataattggtg gagttaccca tggaggcata acggtagctg ctggcactaa
     7321 acctgtagaa gtcgcaaagt aagcaacact aactaagacc attggtgtca aaataaatgg
     7381 aatgaacatg atcgggttca acacgatagg taacccaaac atcgttggtt cattaatatt
     7441 gaagacacct ggcgcaatcg acaagtttgt tacaaccata tatggtttgt ttttacgtcc
     7501 tactaaataa atagctaaca gtaaacctaa agttgcacct gttcctccta agttgacaaa
     7561 gctgtcaaag aaaggtttgt tcacgataaa cggtgcgcta tgtccagctg cgattgcatt
     7621 aacgtttgct tcaatggctg gcgcattgat tgtttgcatc aaaggatcga ccatattcgc
     7681 tccgtgtaaa ccaaagaacc ataagaatgg tgtgataaat gcaagcagta aggcagatgg
     7741 ataagagttt gcaagtccca taaatggttc ttggaccact gtatagaaag aacctacgat
     7801 atcatcaaca ccgaatgcag caaaaattgc agcaactaaa gcaaacgcag agatagtgat
     7861 cattgctggt agtaatgaag caaatgcttt tgcaactgcc ggtggcacac catcaggcat
     7921 tttgatgatc aatttttcat ttccgcttaa acgtacaaag atttctgttg aaataatccc
     7981 aacaataata gcaataaaca aaccgtttga gctcatacct aatgctccgc ctaaagcgaa
     8041 gaaagagcct aaagaaatca cacctgcgga aacaccgtct tttcggtagc cgttagctaa
     8101 atgataagca agcaagaaag caatcaagac agaaagaatg gcaaatgtac cgttccaagc
     8161 agcagccccg aatcctttcc atgattcacc accaaaaatt gagttcatca attcttggaa
     8221 cccgggaatc ggtaagttat tgatcattac tgctaatgct cctaagatca ttaacggcat
     8281 actcaccata aatgagtccc gaatcgcaac taaatgccgt tgtgcaccaa tttttgatgc
     8341 tacaggtata aaatgtttct ccataaatgc aacaaattta tccataaatc ttccctcttt
     8401 tctttactgt agattacgcg ttcaatcgac gatacagatt aatgatctct tttgctaaat
     8461 ctgtaaaagc tatagatgtc atcagatgat cttgactgtg gaccgtcaaa agcgtgactt
     8521 caacgtgatt tccttgtgct tcttgtgtca gcatctctgt ttgagagtga tgtgcttgga
     8581 ctaaagattc ctctgcatct ttgatttttt catctgctaa agtgaaatca ccttttttcg
     8641 cagcagcaat cgcttccatt gcatcgcttt ttgcatttcc gccaaacatg atcagaccca
     8701 tgatggcttc tagatttttt tgatcttcca tttattgtat cctccgataa tttttattat
     8761 ttgtttatta gtttttctgc ttggtcgagt actttttcac cattcatcat gccatagtca
     8821 gccatattga ttacatctaa aggaatgcct ttaggtgcta gcttttcagc aaattgattt
     8881 tgcataaagc gtacttgcgg tcctaaaagt aaaacgtcta catttttaga ttctagttga
     8941 ttatccgcat ctgaagcaga tacagcaaag atgtctgctt ccattccttt tgcttctgct
     9001 gccttttgca tttttgtgac taggagactt gtactcattc cagctgaaca tacaagcatg
     9061 attgttttct tttccatttt aaacacgttc ctttctatac ttacacttgt atataagcaa
     9121 gtagcgtgcc aactttaaaa gcgctttaaa aagttgatat catcacattt aaggtctata
     9181 ccaagttgca tacacactaa aaaagaaagc gcacactaaa cacacaaatt aatttttatt
     9241 accttttttc ttttaacgtg cacaaaaaaa gttgtgacac aaaacttgtc acaacttttt
     9301 tcaaataaac ggtattcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac
     9361 ataagatgtt gtttttactt tgctattctt attacttgat gcaggacagc tttttcacaa
     9421 cctctcaata aacggtgttc aatcagctga ccctgggtat tagctcaaaa cgctggattc
     9481 acaacctctt ttatttcaaa taaccagtaa atacaatgaa catctacaaa aaacttctat
     9541 ttatttatag atttcgtcag gcaaatccaa ttctctctta tcaatcaaag tctgatacca
     9601 cgaagcagat tttttcaatg ttctgcttcg ttcatcgttc aattcgattc gaactaatcc
     9661 atatcgattc ttaaatgcat tcattggtga cacacagtca gtgaatgccc ataacatata
     9721 gccttcgcaa tttgcgcctt cttctgttgc tttgatcaag taatgcaaat gctgtccgat
     9781 aaattcgata cggtaatcat cttgcacgat tcctttctca tccatatact gttcttcata
     9841 ttctcttccc ataccgtttt ctgttatcag ccaagggata ttatgataat tttcttttag
     9901 atacatggct atatcataca tgattttcgg atagatttcc catcccctgg aattattcat
     9961 tttcttccca ggtaaatcaa agacttcata atatcgttca ggatggaaag gtgtctcttc
    10021 attccattga taacgagggg cgcgaacgcg tttaggataa tattgattga ttccaaggaa
    10081 atctactgta tgatgctgaa tcgtttctaa atcctctgtt gcaggatcga aataaatcga
    10141 ttgtttttga cacaactcca ggaactcttc tgtgtagtat ccttttacca tcggatcaaa
    10201 aaatactcga ttataaaaca aatcatacat ataagcagct ttttggtctt ctggagaatt
    10261 ggatcgagca tagaccattt caggatttaa aatacagcca atccttccac cataatttcc
    10321 gctgcggtaa atctctactg ctttagccgt tgctaaaact ttatgataat tccacatcat
    10381 ccactttttt gtgttttgtt catgcggcca gcgaacagca tccaaatagc aacgcgtttg
    10441 gggaacgatt ggttcgttga atgtaaacca gttttttacc ctatcaccgt aacgatcaaa
    10501 ggcaatttga gcatacttag cataaaaatc aactactttt cttgaagacc agccatcata
    10561 tttttccaaa agaaaagctg ggacttcata atgttccaaa caaatcatcg gttctacgtc
    10621 tgcttcgatc aaagagtcga tcatttcact tatatgctga gcatattctt catctactat
    10681 acagttctca taatcagtaa aaaaacgagc ccaattgata gaagtccgat aatgtgtcag
    10741 atggatggat ttcatcagat ccacatcctg ctggtattgt tccataaaat tagttgctac
    10801 ggctgggcca tacccattat gccaaacaaa atgttcattt ttataccagc tatctataaa
    10861 cgaatcctgt ccttcttttt ttcctttcca accttccgtt tgccaagcag aagatgcagc
    10921 accaagaata aaattttttg gaatagtaat tttcatgatt tcctcctgtt ttttagatcc
    10981 catcattttc cacacgattt gctactttta caaatggcag atagatcaaa atagaaacaa
    11041 taatacaaac aagttgcgta ataactgcac cgatatttcc tgctgttgcc agataagcat
    11101 tgacgatcgg cggcattggc caagggacca ttacaactgc tttcgccgca aatcctaaag
    11161 cggttgcaaa ataaccaatc gttccagtga taagaggcgt taagataaac ggaatagcta
    11221 aaattggatt cagcatgatc ggcataccaa aaataactgg ttcattgatg ttgaagatcc
    11281 caggagcaag ggataacttg gtgatttgtt tcatatcttc ccgttttcca actaacaata
    11341 ctgcaatcag taaaccgatc gtgactccag acccgcccat actcatatac atatcccaaa
    11401 atggcatagt aataatgttc ggtacttctt ttccagcttc aaaagcttcc gtatttacag
    11461 caattgccgc taaaagcaac ggttcccgta tcggcttcac catttgattt ccatggatac
    11521 caatcaccca aaagacttgc gcaataaaca tcaatagcaa aatacccggt aacccttgta
    11581 cgacaccttc taatgggcgt tgtacgacat tgtaaatgat atcataaagg tacattcctg
    11641 taagttcttt gatgataaat cccgcagccg cgatacccga tacagtcaga atcgtcggga
    11701 ataaagcaga aaaactagca gaaacattcg ttggcacggt atcaggcatt ctcagctgta
    11761 gtttcttctg tttcctcaac cagcaataaa gttcaataga aaggatggag accaacattc
    11821 ccaaaaataa ccctttcgta tctgtatatt gtttagccaa tacgttttct actataacag
    11881 tctgattttc attcactaat tcataaacag ttggattaac agataaataa gacatcactg
    11941 cgatgatacc agggaataca ccatccactt tattcagctt ggctaattcg atccctatca
    12001 gaaaaacagc atagatggtc aagaaactta gtgtgacata gctgatggca gatgcgatag
    12061 gctttaattc ttctaaaaaa cgcaagaaac ttatctgtgc taagccattt tccgagtcaa
    12121 agaccattgc tgaaaacaat gtgccaaaag cacccgtaat aattaccgga atcaatgcag
    12181 caaatgcgtt ttttatcacc atgatatacc ttaaattatt gattctcgtt gccacgttcc
    12241 ctaaaacatt ggctaattta tccataaaac tcattccaga tcccctcatt cctgcttctt
    12301 ctccttgacg tcatctcttt ttgtctatta gtcagcgctt acatttcgat gaacagaaac
    12361 tatttactcc tcttttttac caaatataaa acaaaaattc atctgatgta tttatcataa
    12421 aatacatcag atgaactgtc aattagtttc tttttctcct attattttct gtaattctct
    12481 ccgatttatt gccatatgga cagtcattgc atcaaacgca gcaagtgcat tccgcctttc
    12541 gatggcctga acgatctctg tatgaatatc gatcgtctcg attttcagat gctggatatc
    12601 ataattttta ttaaacaaag caattgattc attaatgatt gggataatat gcagcaaggc
    12661 tacgtttccg ctagcttctg caatcatcgt atgaaaatcg atgtctaatt gaacatgttc
    12721 ttgtgtgcct tctcgaaaag aagtttcaat cttttgcgtt aattttttca tttggacgat
    12781 ttgattcttc gttgcatatt ccgctgccaa acctgccatc ttaggctcca ataaatatct
    12841 cagttcaaat aagtctcgta tcatccgatc ctgatcttca atcaaagaaa aaccgagtgg
    12901 gtcattggca atccctcgct tatcactgac aaatgtcccc gctccttgcc gaacttcaag
    12961 gatattcctg gaagctagag cccgcacagc ttctcgcacc gtgcttcgcc ccacctctaa
    13021 ataatcggac aattcatatt cattcggcag tttatctcct acttgcaact gcatttcctt
    13081 taaatatcta aggatcttgt cagtagttac ttctactagg gttttatttt cttccaaacc
    13141 acatacctcc ttttcatctg atgtattttt tcgtaattat tataacacgt ttgaatcatc
    13201 tgatgatttt ttgtatacaa aattgattaa tccgttcata gacatacaaa cgaagaagat
    13261 atttaccata aaaaagacta ggataaaatt cactcctagc cttcgatcat ttttattttg
    13321 aacgacaaaa aatatgatgt tcaataacag acgatcttca ttcgattctt gatgtcatct
    13381 tttgtttttt ttaatatatg gataaaaagt ttgattacat cgtttctatt ctagaatttg
    13441 gtcgattctc aattcttctt ctggtgagaa atctaaatta tccatcattc gaacactttc
    13501 ttgtaactga cttaaccgac ttgctccgac cagtacactt gtaacagatt tttgtcgcaa
    13561 attccatgca agggccattt gcgctaatga ttgtcctcgt gatacggcga ttgtttgcag
    13621 tgctttgatt ttttccagag ttgatccaac ttgttcttct gaaaggaatg gtatttcctt
    13681 acgatgtgct ctagaatctt caggaatacc atgtagataa cgatttgtca gcaacccttg
    13741 agcaagcgga ctgaacgtga ttgcccctag cccttcttct tctaatactg gagatagatc
    13801 gtcttctagt agtgcacgag aaaacatatt gtagcgcatc tgatgaataa tgaacggtgc
    13861 ttcctttctt tttaaaattt cagtcatttt ttttgtatct tctccattat agttggatat
    13921 accaatatat aatgcttttc cttgtcttac aagttgcatc aaagcttctg ctgtttcttc
    13981 caaaggtgta tcagggtctg ggcgatgatg atagaagatg tcaacataat ctagccccat
    14041 tctttggaga ctttggtcac aacttgaaat gatggattta cgagagcccc attccccata
    14101 tggcccttcc cacatatgat aacctgcttt gctagaaata atcagttcat cacggtaggc
    14161 ttgaaaatct tctctaaaaa tacgcccaaa gtttttctct gcagctccag cagggcctcc
    14221 ataattattc gcaaggtcga aatgggtaat tcccatgtcg aatgcgcctc ttaaaatctc
    14281 tctttgatta tggataggat cataatcacc aaaattttgc cataatccga gagataaaat
    14341 cggtaatttc aaacctgaat gaccggattt acgatattcc attttttcat aacgtgcact
    14401 atctgcaata tacaattttt ctcctcctca tcaaatcttt ttaaaacgct ttcttttttc
    14461 tatttttagt ttactacatt ttaaagaaaa ggagttaatc aaaatgattt tttcgtatat
    14521 ttttttgcca tttgtttttt actcgttgat agatcagaaa ttgattacgt gcttgctgga
    14581 tttttttata acgtttcggt tgttttttgt aaaattgctg atgatagttt tctgctggcc
    14641 aaaacgtacc ggctggttcg atttttgtga cgattggttg gtggaaagtt cctgaactgt
    14701 ctagtttctg ttttgattgt tccgctaatt ctttttgtcg ttcgacttga taaaaaatca
    14761 ctggacgata ttgcttccct ctatcttgaa attgtccaaa tgtatctgtg ggatcagtaa
    14821 tttgccaata gattgtcagc aactcgctat aacttatttc tcttgtgtca taaatgatct
    14881 ccacagcttc cacatgacct gtgtaaccac cacttacttg atcataattt ggtttttcaa
    14941 catgtccgcc cgtgtatcct gacaaaacag ataggatccc ttttttcgat tcaaacggct
    15001 cgaccataca ccagaaacat ccccctgcaa atactgcacg ttcttgatag tcagcatctt
    15061 gcatggcatg cttggaaaag tcatattttt gctccttatc aaatccttcc gtaatcaata
    15121 aataaaagtc cattacatca ggcgtcagat tcattcggat agctaatgga cgtagtgttg
    15181 cttctagttt tgataactgt tctttaacgg atatattttc tcttatgtca ttctttgtct
    15241 gaatcaatac ttttcgttcc caatctcgtg tattcggatt caaaatcaga ttataaagtg
    15301 tttgaatgaa tatttgttcc tcatccatag attctcctcc tttccgaaga agacaagcta
    15361 gtgactttag ctgtaattta tcataaagtg aggaaataag cgattacttc atccccctac
    15421 agatttttcg tataaaaaag agagaatcct tatcgacaga ttctcctttt taatcaattt
    15481 attttataaa tgaaaagtta atttaccttt tatggaaaac tattttaaaa aataaacata
    15541 aatatgcagt taatgtaaca agataagaaa taatagtcgt tactgtataa cttgtgctta
    15601 ctttaatatc agcaactcct ttgactccgc ttatttgtac caatccatta gcattcacct
    15661 gctcctttac aggttgatga tttatttcta cattcgttcc taaataagca tatacaggaa
    15721 gtgtgattac ttggctcttc tcattagtta ctctatatat gatagaacta ccagttgttt
    15781 tataaggaag gtttacccat tgactgccaa tctttgcttc gtgttttatt atatgctcct
    15841 tattctccaa tgaagcgata ggagaataat catataaaaa ataatccatt cctttagtgg
    15901 tatacgcctc tactgtttct ttgtttacaa caatatgtcc cggctgagaa attatttttt
    15961 gattgaaatt ttttttacta gaaacagtta aagtgattaa taacaaaaca gtcataaata
    16021 aataaaatag ttctcttgtc ttattgtatc ttctattttt gaaaaatatc atcgttaata
    16081 tcagagaaga gaatattatt tgcaagctta agatccgcca aggaaattgt aacaatttgg
    16141 ctggcgtatt ttgcagaaga taccatggca ccaaactagt tgtagctagc caagtcaaac
    16201 acgtaccata ataaatcttt ttttctatgt ttgttaattt tttaaataaa atcaggggaa
    16261 tacataaaga taacagacca actagtccca ttgagtaagt agttaagtcg ttgttcatag
    16321 cttcagtcag actatcgaac acattagatg catgagcata taaatttgtc acgctaggtt
    16381 tgttgatttt ttggtagagc atttgttcaa acatcggata ccaaaaagca ctcgtcagaa
    16441 ccaatgtcat tcctgccgca ctaaataatt ttttaaatat agcaataaaa tctgcattct
    16501 tgaaaccttt agaaaccaaa cgaataaaaa taaaaatgat aattaatgct gtactcatta
    16561 atgttgatag tacatggctg tacactagca aactcatact caatgctagc tttacagacg
    16621 gatacttttc ttcttttcca gagatgattt gataagcata aaataataca gggataacaa
    16681 gtgttattgc gatagcttcg gcaattgcgg aacgatagta gatgtctact gtccgataag
    16741 cagagaaaat atagagacaa ctaaataaaa atgccgcttt tttttgtttt aaaaaccgct
    16801 cgccataatg ataagctacg agtgttgtac acaagttcaa taaccaaacg taaataatat
    16861 agcttacaat caaatttttt gtcatccaat ataacattac agctggaaaa aaagtcagaa
    16921 agggataaaa ataatttaca ccatatccag tataattgaa tgtctcataa ttaatcggac
    16981 tggtaaaaat agtcgataac cccttgattc gagataagtg aaaagaaaga tctccagtgg
    17041 tataaatact ctgtgtattt ttgaaaaaga tgaaagaata tatactacta actataatta
    17101 atccactcaa tattactact tttttattta ttttcattct tcttgtttat ctccttttta
    17161 gtcacatatc gagaatcttt caacttatta ggtgcaaatc gtttaaaact tgaatacacc
    17221 actaaacaat aagtcagaat actgatccat aaagccatga gagcagttgg agtatatttt
    17281 gttgtaattt ctatcttatt ttctccagca tttacatcca cattcactaa gccatgtttt
    17341 ttataagagt catttacgac tttaccgttt acttttacct gtgtacctaa ataagcatat
    17401 actggtactg tcactttttg ctttttgttg cttgccactg tgtaagtaat gttattatct
    17461 gcagctttat acttcccttt ttcccacgat tctcctactt ttacttcatg tttttctaag
    17521 tgaggccttt cttttatcgc ttctgttggg gcgtaatcgt ataatcctcc catatctgat
    17581 gttgtataac gtgggagtgt ttcttcattg ataacaatat gaccgtgttt tgattggata
    17641 acttttgcgt aattttcttt tgctgctata gacgtgacga gtagaaacac cacagtagcg
    17701 ccaatgatta accatgaata ttttttattc atagtctttt tcataaaaac aagcgttatg
    17761 atcattgaag aaaaaaagat ttggatacct aagatacgcc aaggaaactg gagtaagctt
    17821 atcggtgttt tttgaaacaa aaaccatgga aataaattag ttacgcatac ccatgtagaa
    17881 actacgccaa catagatgat tttttctttt ttctccattt ctttcagtat gaataaagga
    17941 aaaatcaacg caaccaatcc taataacccc atagtgtatg ttgtaatatc gttatttaac
    18001 gcttgaatga tactatctga aatagaaagt gcttctagct gcaaatctgt tttgaaggga
    18061 ctgtgtattt cttgataatt catctgctgc aacataggat accaaaaata gctagttaat
    18121 gcacaagtca aaaaaacagc ctttactaaa ttcacgacca caaattttat atcgtttttt
    18181 atttgcttgc ttaaaaacaa acgaactata atcatacaag caatcaatgc actacacaat
    18241 agtgtagtta agacatgagt atacacaagt agacttatac tgacagataa agcgatgact
    18301 tttttttctc tattgaatat caatccataa gcataatata aaacagctgg gataatcgta
    18361 agagcgattg cttctgcaat tgcggatcga taatagatat caactgttcg ataagttgca
    18421 aaagtatata aacaactaaa taaaaaagca gcatctatct ttttaaaaaa cttttctcca
    18481 taataaaaac taatcagaat cgtacaaata tttaataacc aaatatatag aacataactt
    18541 aatattaagt tatttgaaat tccataaaaa acgactgcag gaattattgt aagaaaagga
    18601 taaaaatagt tcagtccatc tccataattg ttaaatgttg tatagttgat tggcccttca
    18661 aaaatagaag ataacccttt gattcgtgaa agatgaaaag ataaatcacc tgttgtataa
    18721 atttcttgag catttctcat aaaatttact gaatataaag tactaagtaa gataagaact
    18781 actaaaagaa atgctttttt acttattttc atagatttgt tgttctcctt ttttaattct
    18841 attttcagta tactagaatt gtcttttttt aataagtttt gcgttaaatt tttcatagca
    18901 ctagtatgat atactagaaa agaatcaatt tttattactt ttaaatagga gaaaaaaatg
    18961 aaaaaaaaca aaaaccaaat aatagatatc atttttatgt tatttctaag ttttgcttac
    19021 gtcatccctc tattcatgtc taagggaatc tatcatagtg tcaatcagga tacttacttt
    19081 catttatctc gaatcatagg actagataat gtatggagtt ctccagtaaa cttcaataac
    19141 ttcgatcatc atggaacaat gatgaatatt ttttatccat ggcttactct ttatccagct
    19201 tttttatttt ataaaatgat gggaaatctt gttttaagtt ataatatcta ctattttttt
    19261 atcacttttt taacaatggt cgtttcttat ttttctatga aacaaataaa aaacaatcgt
    19321 tatatttcgc tgttattttc gattatttat acattttccg cctatcgtgc cattgacata
    19381 ttccatcgtg cttcattggg agaagctgtc gctttaacat ttttgccttt gatactaatg
    19441 gggtgttacg aaatttatat tagagattac caaaaatggt actggttgtc tattggcatg
    19501 acgctagtag tgtatactca tttactttct gtcgctatgg tttctgtatt tattggagga
    19561 acgttgtttc tttcttttta tttttgggac caaaaaatcg ctagactctt aagtctttta
    19621 aaagctaccg tcctcacctt ttttttaagt gcgggattct taattccttt tattcaacaa
    19681 tctagagcac aagaattgaa agtcccactg ggaaaagaat taagtggtat ggctcctagt
    19741 gatatgctga cacatatttt aaataataac tataacaact atactattgg attattctta
    19801 tttctcggat tgattggtgc ttttattttt ataaaaaaat taactgctga tgatttattt
    19861 attttctttc ttggtgtatt tgttcttttt tgttcaacca atttatttcc ttggcaactt
    19921 tttaaccata caccagttaa gagtctacaa tttgtctggc ggttaaatgg tttttcttct
    19981 ttatttatag cctatacgat gagtatagtg atctattata ttttttctac taaaaataat
    20041 tcatggaaaa taatggtctt tacgtttctt gcactaatat tgcatctctc aggagtaagc
    20101 aattctatcc atgctaacaa agattcatta acacttatag cacctgaaga cgctgttgct
    20161 atagctgaaa attacaatca tactgattat gcaaacaaag agtcaatcta tcatcctgat
    20221 atgattaata atgatcaata tttactaggt aaccaggaaa taaatccaac aactcatttt
    20281 atgagtaaca aacttacaat tgaattagat aattcgaata ataaaaatac tgtcttgacg
    20341 acacctattt atcgttataa agggcaagtg gcttctatta atggcaaact agtacaaaca
    20401 aaattatcta agtttggcac aactgaatta actattcctc caggtataaa taaagtcgtt
    20461 attacttatc agtacacaaa actagcaatt gcttcaagat atttatctat cgttactttg
    20521 attttatttt tactttatcg atttactttt tctaaacaaa aacaaccgag aagagaagta
    20581 caacattctc actagatttt tatttaagaa agacttttta gctgtacaca taaacaggct
    20641 aaaaagtctt tttttatatt tctttaattt gcttcataca ttatttaaca tcgatattta
    20701 tactgtgaaa catgcgtttc atccattatc aaagtatttt tatcgataat aaacttcatc
    20761 caaaaacaga ccttgtgcag ggacggtttc tcctgcatgt tgccgcactt tgctttcaaa
    20821 tatttcatcg attgtttcta tcggcatggc acctgtgccg atttctagta atgtccccat
    20881 gataatccgc accattttat ataagaatcc gtctccgaca aaagtaaaat gaagcatttc
    20941 tccctttcgt tcgatagata attcatcaat cgtacgcact gtggattttt ttgatttttt
    21001 caatgcagaa aaaccaatga aatcatgtgt acccactaat ttttcgcaag ctgcattcag
    21061 cttgtccata tccaactctt gagggaaata aaagctgtgg tttcgctcaa atgctgaagg
    21121 aatcacgttg ttccaaacat aatagctgta ttttttgccc accgcattat atcttgaatg
    21181 gaagcgctca ggcatttctt cgattctttt gatgacaata tcccgcggaa ggtaacgatt
    21241 cataaagtcg atcatttctg ccagtccaat ttctgtgttt gttttaaaat ttgctacttg
    21301 tcctcgcgca tgggtgccag catctgttct tcctgatcca ataatttcta tctcttcatt
    21361 tgtcatctga tgcaatactt tttcgatttt tccttgaatg gttttatcgt catctccaag
    21421 tctttgccat cctgaatatc tttttccgtc atattcgatt gtcaacttga tgtttctcat
    21481 attttagtgg ctcctctgtc ttccatttgg tcttctctct acctttttaa gattgtagca
    21541 caaaatatag ctccttgctt ctctaagaca gaagctatac aagaaaactg tggaaatata
    21601 tatttcagaa aaacaaaaaa cagccaccgg atgaagtatc cgctgactgt tcaatttgac
    21661 ggctcaagtt ttactgtttg agatgcaaga tactcaacct ggcaatgggc acataattaa
    21721 agaaaaaact gattctttgg caaaaaccta ttttattcag taatggccaa gaatttatag
    21781 aaggaattct tgccagtcca taaatcgccg caaaaagcaa actgatcaca gacagtccaa
    21841 ggtataagtg gcttacgtcc atttgcccat tttttcgata gatcaagaaa agaagaactg
    21901 gaaacaataa aaaaccactg taacctaaac ctgatccgat attatggacc catgtcgaga
    21961 cattccacga agattcatgg gtattgatac tgaacaaacc tgtaaagata caatcaccta
    22021 taccatagaa cgcgatggct gctgtaacta gggtagctaa cacacgagat gtactaacaa
    22081 atgcagcata caatgcaggc aatgacagga tgaaaaagat ccctgaaacc acagaccaaa
    22141 taagaaacgc ttttcttaca ggactggaaa cctctccaaa tacactgatc acgctcttca
    22201 tttgatctaa ttcaggataa tacaatccta atacgtacgg agtcaaaaaa tcactcagca
    22261 cgcctaataa taaaaagaaa aaaccaaatt tacgtaaaaa atccatgata cgcctccaat
    22321 aacaattacc tatgctttta attgttatta taaacgactt tatcttgatt taacagaaac
    22381 ctctatttac acgaacatct atataactca ttaaaaactc gaatcatttt aaaaatacta
    22441 aatatctaac gttcaacgtt cacttaatcc atctaaacaa tctttcaatt tgattttttc
    22501 cccacttctt ttacgcgcgc ctagctctcc tattgcagta aacaacagat cacttgacga
    22561 atttaaggca gtttccattg aatcctggat aacaccgata ataaatccaa taccgacgat
    22621 ttgcatcgcg acatcattag agataccaaa caaactacat gctaatggaa tcaataacag
    22681 tgaaccgccc gcaacacctg aagctccaca agcagctaaa gcagacaaaa tacataaaag
    22741 gactttcaaa acaaaaggtg cttcaatccc taaagaatgt acagcagtca acgtcatcaa
    22801 ggaaatggta atcgcggcac cgcccatatt gatcgtagca cctaacggga tcgatatgga
    22861 ataagactct tttgtcaatt tcaatctttc agccaacatc atgtttatcg ggatatttgc
    22921 ggcagaactt cgggtaaaga aagcgggaag tgcgctttct ttcaaacaaa agaaaatcaa
    22981 tggataggga ttttctttaa taaacaaaaa cgtgaggaat gggtacacaa ctaatgcgac
    23041 aaacagcatc gtaccgatca aaacaagtaa aagctgcaga tactctgcca atccagcgat
    23101 tccagtcgta gcaattgatt gataaaccaa tcctaaaatc cctatcggag caaatgcaat
    23161 aatcacttgt acgacttgtg acaacgctat agatatctga tccaccaact cttttgtcga
    23221 ttctgaacgt gctcgaaaag ctagtcccaa caatacagcc cagaataaga cgccaaggta
    23281 attcgcatca ataagagatt gaacaggatt ttgtgtgaca ttcgtcaaca tagtagataa
    23341 aacactttcc aaactttgcg gtgctttttc agatactgtt tcttgtaaag taataggaac
    23401 agtaaaaatt gaacttacaa taactgctac aagtgcagaa agcaatgttc ccactaaata
    23461 taagatcaaa ataggcttca cgaatgtctt attcccaatt ttatgcttgg caattgacgc
    23521 catcgtcaag aaaaaaacca gaataggtgc gattgccttc aagcaactga caaacaacgt
    23581 tcccaatatt gaaataaatg tccatgtggg gaaaaagata ccaaacaata ttccgactaa
    23641 aataccaaca acaatacgtt gaactaacga catatccaaa aacttctcta acaaatgacc
    23701 accctttcac aattttaaca atcattgtat cattttatgc aaagaaacta aaggtataat
    23761 tttctaaaaa ttctaacaat acaaacaacc atttcttgct aattttgtac tttttctgtc
    23821 tacacgttgt tttttgagat attctaagtt aggatgttat taatgggcaa cttatcagga
    23881 aacgttttct tatgtttttg tccaattttt ttgtgaggtg tttattcttg aaaaaaataa
    23941 agaaggagag ttatggcttc ctgacaatct ttgtcgcttg tataatgatg gttttacagc
    24001 gtatattgat ttcagcagtc actattccta catatgcaac aaaccctggt cgattctaca
    24061 tctatacatt tttacaaact tccgttgtta ttggtgcaaa tttgatccca ctttacatgg
    24121 gttaccaata tcaaaaaatc gcccgtttaa aagttttaca ttatttaagt cgttttgcct
    24181 ttatattcct aatagccaca ctagtttgca atatcttttt ttacgtacaa gctggcagct
    24241 tgaatattcg tgattattgg cttatcctaa cacctatcag tcagaattac tttacttatg
    24301 ctgtttcttg tactttgctt ttttgtagta ttccttatac agttggattt ttaaataaat
    24361 tatcgaaaac aacaattaaa tatctactat tatttttaac aattggatta gttggctgct
    24421 ctactctatt taataaagac ccgtttgcat tacagaatgg acagagtatt ttatggattt
    24481 tttatttatt tttaatgggt tatggtttga aagaatggaa ctggaaagaa aaggtccgct
    24541 ataagcttcc tcaagtattt atcagcttga ttgttttatt cggtttgatt attttgatga
    24601 cacaaatttc actcatcatc agaggaaata cagatacagc gctgaggttt tctaaaccat
    24661 attctctctt cgcaatgtat tatactttct cttcgttttt atttttagaa atgctatctg
    24721 ataaattagg tataaaagga agaggaaatt ttcttgctgt atttttgatt gtcactcaag
    24781 ttatatgtaa tttcccttta gtttcttatc gcgtatctac atttatgaaa cgtgaatttc
    24841 caaactcagg gaaggcatgg ctgataaata tcgggcagtt ttttatttga tatttattag
    24901 cagtgtccct cctagttatc tttttatttt atatacaaaa aatccgtttc attcatcgtt
    24961 tcatcgaata cttcgctttt gattcgattg aacaattaac tcagcgttgg aaaaaaagaa
    25021 ttcattggct ttttgttcat aaaagttatt ttttagttgc agcatttttt tactgcttta
    25081 ccttcgtgca gattttcgtt ttagaaccaa aagaaggttg gaaagaaact atccaggttg
    25141 ccttaaaaat atttactcag cgacaagcac ccatgatcct gacaatcatc attattatgg
    25201 gatttttctt cttactattg ttgatttcca atcgtttttg gtacgccttg actgctacct
    25261 tgatcattaa cttactgctc actatttcga cagtaatcaa aatggaaatg cgggaagagc
    25321 ctgtctttcc ttcagattta aaaatgctga ccggattgtc agaactatta tcgatggtta
    25381 gcccagtctt attaatcgtg ggcggattga tccttctcct attattgatt actagtatta
    25441 ttgtacaacg tagactacaa aaacagtatt ccttgaagat tcattggaaa cgtcgattta
    25501 tcagtatcgc tgtattatta ggatgttttt cgggtgtctt tttcattaat cacaaaaatt
    25561 cgccttcttt cttgctgttt aatttattta aagtaaacaa gactttcttt aaccagcaag
    25621 gcgccgtaaa agataacgga ccagtcattc aattcctaaa caatatcgac attaaaatca
    25681 tggaagaacc tgctggctat tcgaaagaaa aaattaatgg aataatgaaa aagtacaaca
    25741 caaaggcaga tgaaatcaac caaacaagaa gtgactggtt aaacaatcag acgatcgttc
    25801 tcaatttaag tgaaagcttc agtgatcctg cacgtgtccc taatctgaca gtacctacag
    25861 accctattcc ttacatcaaa agcattataa cagaaggaac aggaggattg atgttatccg
    25921 tcggttatgg tggcggaaca gctaacatgg aatgggaagg actgacaggc ttgagtatca
    25981 gtaacctgtc tgcttcgtta gttacacctt atacccagct agtggagcgt cagaaaataa
    26041 gtccaaatat taccgatcta ttcgatgaaa aaatagccat ccatcctttt acagctgctc
    26101 tttatcgcag aaaacaagtg tttgataaat ttggttttga caggttctat tatttagaca
    26161 gtccggataa attaacttat acggataaaa tagaacaaag cccacgcata tcggatgatt
    26221 cagcatacaa agaaaccttg aaagctttac aaagcaatac ggaaacgtca caattcatcc
    26281 aattatccac tatgcaaaac catatgcctt atgatgatta ctatagtgaa ttgaactaca
    26341 ctgctgaagg aactgctgtc attcctaata gaagaaaaga attacaaact tatatgcaag
    26401 gccttcacta taccgataca gcagtaaaaa aattcatcca agaaatcgac aaaatcgata
    26461 aaccgattac ctttgttttt tatggtgacc atttacccgc aatctatagt gggaatgatt
    26521 cgaaaaaata tggattagaa cagcaccaga cagattattt catttataac aacaagtata
    26581 gtcgtgaaca agcagctacg ttaaataaag aagttgttgc tcctaataac tttggtgcta
    26641 tggctcttga gcatgcgaat atcaaagtaa ctccttacta tgcgttgttg acagaagtat
    26701 ctaatcatct acctgcatta acaattgatc ctacagaaag cttgtctaat cgttttaatg
    26761 gaaaacaggt ctttgttaca gaggatagtc aaattatcca agaaaaaaat ttaactgaga
    26821 aacaaaaaga aattctagaa gattatcgtc tgatacaata tgatcttgtt gcaggtaacc
    26881 agtacagtgc aacatgggcg acacaaaaaa taaaagacta acaaataaac tgagcaaggg
    26941 gttgtgataa aagacatgtc acaacccctt gtttatctca ttaatttgct tgtccataaa
    27001 cgatttgatt aaagccattt ttcaattcat tattcgctgt ctcgttgaag taaagataat
    27061 tatcgggatt cccctgattt tgttgtgtca ccaatacttt tggctgattt tgctgaaacc
    27121 cgaatagata gacgtgcttt ttcagatatg gttctgtctc tgcatcggaa tagacggctg
    27181 tgatttgata gtctgcttga cctgtcccac tttctgacca ttctgcagaa acaggcactc
    27241 caccaatagc catcttccac tccccgttca aaatagcttg agggacttta agaccatata
    27301 agtccacact catttgattc gtataacttt tgtattgttg tcccattgtt tggccccatt
    27361 tggtcataaa tgcctctaat tgtgcggatt tgtctgcatt ccacagtgtt tgttcaactt
    27421 gttcgctgct ttctgaagag gaagatgaag cctctacaga agaacttgaa gtagatgttt
    27481 gagttgtttc agtcgttttg gaagactctg acgaagtaga ggtatctgct gtttctgtac
    27541 ttgctgcagt tgacttgctc tctgtgctag ttgttgtagt agttgcaact gttgctttcg
    27601 ctgtttcttt tgttgattct tttttcgatt cattcccgtt ggtacaacca gctaacaaaa
    27661 aagctagacc aattgttact aaaatccttt ttttcaatta aaaaactcct ttcgattact
    27721 cccacttctt tattctatca aataagcaat gtgattttat tgatttttcg tattttctca
    27781 tcaatctttc aaatcttcta aaaactgaga agcacccgct gatgttgtct gtaatacatc
    27841 agcgggtgct tcttttattt taatcttcgt gattttccaa tttacctcac gctttatatt
    27901 tgtatttgaa gaaacataga cttttagtca tcatcgtctt cctcatccaa atcttgtcgg
    27961 acagtttttc cttggagtct ttgtacttgg tagatgattt ctttattgaa acttaaaagt
    28021 tcaacaagca attgttgcaa gccatatttt tcttcttttt cagctaattt gattgcccgt
    28081 gtgataaaga cattttggaa atcaaaatct tttgcaaagt tttccaacat tgttgaggct
    28141 ggtaaatatt tgttttgacc atgttcgtaa atttttccat aacgcatgac ttcttctaaa
    28201 gtagacggaa cgatttcatt ttcaatcact aactcatgag agattttatt gaaccatctc
    28261 gcttcttgat cagcatattc tgaaaaaagg gtacttaatt cttgcgaaga tgtacccaaa
    28321 acatacaatt gtgcttggat caatttcatt tcttctaatt taaaatttgc taatacatgc
    28381 cccaccatgg cgccagcagt tggtttatga tggtcgatat ctgcttgttt tgtttcttga
    28441 acaaattttt cttccactat attcattgat ttacgcctct tttacttttg ctgattggac
    28501 ttcataacct aatttttcca caactgcagc taattgttca gggtggactt catctggatc
    28561 aaagtttgct ttgattttgc tcgcattaaa caaaactttc gtattgctta ccccaggctg
    28621 ttgagaaacc gcatgttcga ttttttgcat acagcttgga caagctagtg tatctaattg
    28681 cataataatt tttttcatcg tagttcttcc ttcctttttc ttgatagtct tattctagat
    28741 tacagcttcc ataaaaaaat tgattcttat caagttttac gatttttctt ctttgctttc
    28801 gtgtccaaaa tacaataaac gcatcgcgtt aaagataacg atcaagatac tagcttcgtg
    28861 aacgaacatc ccactagcca tgtaaataaa cccggcaaat aagccgttga gtaaaaataa
    28921 gacgacacca atggcaatca ctacattttc acgggtattt aaaacagttt tcttagccaa
    28981 gcggtaggca tgaaccaacg attcaaaatt agattgcatc aagacaacgt cactcgtttc
    29041 gatagcaaca tccgttcctg atcccatagc aatcccgata tcagccgcag ccaaagaggg
    29101 agaatcattg attccatctc caataaaagc aacgcgcaat ccttgttctt ggtatttttt
    29161 cacaaattga accttttcgt ctggtaataa ttcagcatgg acttcatcaa tgcctaacat
    29221 atcagctaca taatcagcag tcatctgatt atcacctgtc agcatcacta actgttttgc
    29281 tcctgcttgt ttcaacttag ctaattgttc agcgacttct ggtcgaatca catctgacac
    29341 acctatgatt tgagtgattt ttttgtcgat tgctacaata atgaccgagc tgcctgtttg
    29401 ttgtaattga tgtaaatctt gtacttgctt tggtgtgaga gaaagtttgt gagcggcaat
    29461 caatttggtg ttgcccgcac aaactgtatg accatcaatc tcagctgtga ttccttgtcc
    29521 cttgattgtc tgattatctt tgactgctaa ttgttgaaaa tcaatgtctt tctcctgagc
    29581 ataatggatc acggcgcgcc ccagaggatg atcggataac gtttctaatc gagccactaa
    29641 agctaagctg tccccttcat cctctgtata gttcttgata gtggatacag cagtagttcc
    29701 tttggtcaat gtaccagttt tatcaaagac aaaagtatcg attttggaaa aagtatccat
    29761 tgcttcaccg cctttgacta agatgccccg tttcgctcca ttaccgattc ctgctacatt
    29821 cgaaacaggc gcaccaatga ctaaagcgcc aggacaacca agaaccaaaa cagtgattgc
    29881 taaacgaaaa tcacgactaa tcagaccaac aaccaacgca ataagtaaaa cggttggagt
    29941 ataatactga gaaaatcgat caatgaattt ttccgcatgg gatttggtat cttgtgcttc
    30001 ttctactaat tcgataattt tggcaaaggt cgtatcatct ccgaccttag ttgcttcaat
    30061 cgtcacgtag ccatctgcta aaatcgttcc agagaagact tcatctccac tgattttgac
    30121 gacttgtcgt gattcaccag tcactgcggc ttcatccgca taccctttcc cgtcaatgac
    30181 tcgtccgtcc acaggaatag ctccaccagt ttttactaag acgatatcgc cttcttccac
    30241 gtcatcgatt tccacttctt cagtcgttcc atcttcttgg acaacactag cagtagatgg
    30301 ggccatctcg gtcaaagcct tgattgactc ccgcgttttg gtcaatgttt tttgttccag
    30361 atagctacca aacaaaaaca agaatgtaac gatagctgac tccttgtatt ctccgatcat
    30421 aaaggcacca agcacagcaa tagtaacgag taaatcaatt gagataactt tgactttcac
    30481 tgcctgatac gcatggatca taataggtat gaccgcaata atagaagcta tggctagagc
    30541 aatgttatag aacaagacaa aaccagtaaa ttttccgata aaaccaaaaa taatcaacac
    30601 agctgataag gcggtgatat gatttcgatg ctcttggatc cacttttgta atttcatctt
    30661 tctccctctt ttcctttcat tcttacgaga ctaagtatag atacttatcc atcataaaaa
    30721 attgaccact atcaagttta gtcaaaaagg cggttttctt ttggtagaat acactacaaa
    30781 aaatagttag caagaagact cactaaaagt gatcttgcca actattgtcg tttctatcga
    30841 aattttcatt gaatgatttt cctgtacagc atacatcgga gatctatttc tttatctaag
    30901 atttttatct tgctaatttt ttcctttagt ctttcaaatc cacgtcgttc atagaattga
    30961 tacgtacagc cactatctgt aaaaaggaat atttcttttc ctttctctct tttttcaaat
    31021 tctgttagca acttggtacc gatgcctttt gttttcaaat caggatttgc agctaaaaaa
    31081 atgatctctc catctggcga atgcttcttt aaatatgctg caagcatttg ttcatttgct
    31141 gtctcgtaag ctcctttact ttctttagca aatatatttt gtaaaaaatc gaatatctta
    31201 acatatattt gctgtccaaa agaacgatgt ttcttttttt ctccttttat ctccgccagc
    31261 aaaacacctg ccaactcgtc ctctgcatac aaagcaagaa tttgagttgc tcgattcaac
    31321 tccatataca aaaaatatct tccgtaaaga ttcagcagaa gttcattttc catatatctc
    31381 tcaaagttca ttccttcaac agcaaaatag atagcttttt tgtaatcttt cttttctaag
    31441 ggtttaaaag ttagtttcat catttcgcct cctcaacttc aatgattgtg ttatacttac
    31501 attataatag acacagtgtc tactatctat aatcaatcat ctataagttt gtcgattaag
    31561 cgacagcttt gctttgatat gtcggataag gagataggta aatgaaaaag acagatagac
    31621 gcgtcaaaaa aacagaaaaa gctttggctg aaacactatc cacattatta gtaaataaaa
    31681 agattcaagc aatcactata cgcgaactaa cagaaacggc agatgtacat cgttcgactt
    31741 tttatacaca ttataaggat atctatgatc tatatgatca attagaaagc aacttttttc
    31801 gggatctaaa tgaaattctc tcttatgatc cagcccatag ctatgaggaa ctatacactc
    31861 gcttgattga ctatgtttac gcaaatactt ttttatatcg cttatttatc ctcaaccatg
    31921 gtgatatttc gtttcagaag aaagttaaac aagttattga agaaaactat ctgaacatat
    31981 ggctatatga agacaagaaa acgattatca cagaagaaat gagattcttt actacgtacc
    32041 acgtccaagg ttgtctatcc gttctcaatc ggtgggtaga aagcaattac aagtactcca
    32101 aaaaagaatt attagaactc ttacagaaac tgaatgatca catagagaaa atcatgcctt
    32161 gaataaaaac atactaagct tagttaagaa gaaccgtaac cttctcgtat aacaactgaa
    32221 tcttatttag ccaaattgtt tactgaagat tttttgagta aaatttctat aattcctact
    32281 tattgaaaag tagtttgtag tgctgcttct ccctctctaa gtgatatatc ttgttgtacc
    32341 aatcaaattc acctcatcat taataaatta cttttaggct aatttattac aacttatagg
    32401 ttgaagctgt aaccaattta tagtcatatt atatttttat catccagttt attgaattct
    32461 tctcgtttac ctcaattcat tttcaagggg aatcagctca tcatctaaca ttttacgcca
    32521 tgctcgcttc atttcttgtg ggtatttaaa tggtttcgct tctggatgtt tggctttata
    32581 tccttcgtaa tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg
    32641 catatattgc agattagaag catgtaattt tttctcagaa acaaaccact cattggaaaa
    32701 gacatgaatc aaatcatcac ttacttgtgt aaaatagcga cgtttaatga caaagatatc
    32761 ttcattgcta tctagtgtac cttcatttct ttgtgcttaa attcaaagat aattttctca
    32821 ataaagattt ttgattccag taagattcct ttactccttt tacaacatag cgttgtcctt
    32881 tgttttgaaa catcttcttc ccctctttgc cattaaaaat agcccaattc tctataaaga
    32941 agattaagct tcattattat aatataaagt ttaaatttgg tttattactt actttttctt
    33001 attctctagt aagctaatat tgaaaaaaat gtaatcagat cattattttt atatgttgta
    33061 acttcctgat aagaaaacta attaaaagaa agaggtaact acataaacta attaaaaaag
    33121 ctaacttcat agttggagga taaatggaag aaacttcaat gattgtttct ccttgtgttc
    33181 cttcaatcga cactagtcct attatcaatc tctctttttt gtccatttac ttttgcgact
    33241 gttccccaat aatcaaatac aggaagcgtt attttttgtc ctttagattc atgtactgta
    33301 tagactattt tcgaatccat cacttgataa ggcatagtat gccattcctt gccaattctt
    33361 acttcatgat tctgcaaatg ttctttgtat tgtaatgctt ccgtgggtgc atagtcataa
    33421 agtcctccgg gaaggagtgt cgtgtaacga ataagatttt cttgattcat taccaagtga
    33481 tctctctgag tgacaacttt atgttcatag tttgcttttg ttgcagcaga tacagccaac
    33541 agtaataaaa caattgccgt tatattaact acttttcttt gcttagctga tgtgctcttc
    33601 atgaaaacga acgataaaat caacgagcca aacagtactt gaaagcctaa gattcgccaa
    33661 ggaaattgaa tcatttgaat gggtgtattt tgtaataaag accatggaaa aaatttggtg
    33721 gataagagcc aagagactac cgcactaagg taaatcactt tttctttgcg attaaatcct
    33781 ttaaaaaaga agagtggaca gatcaaagaa ataataccaa tgattcctac cgtaaaggtt
    33841 gtcaggtcat tattcatcgc accaatcaaa ctatccgaga catttaatgc ttggtgttgt
    33901 aaggtcgttt tatctggctc attaatcgat tgaaccatga tttgtcggaa catcggatac
    33961 caaaagtatg ctgttaaaag aatcgtcatt cctactgcct ttgacaactc cacaatcaac
    34021 cggatgcttt ctttttttct tgtttcactt actacaaagc cgcctaagac tagtaagcca
    34081 acggctatta cactcataaa agtggaaaga acatgtgtat aaatgagtaa gctcatactg
    34141 actgctaatg gaattgcaca tggctttttc tcataaatca gtgcgtaagc ataatacata
    34201 actatcggta taaccgtaag tgcaatcgct tcagcaatag cagaacgatg ataaatatca
    34261 atcgtacggt acccataaaa tgtatataaa cagctaaata taaaagccgc atcgattctt
    34321 tttaaaaatt ttagtccata atgaaacatc agcatgatag tacaaacatt caatagccaa
    34381 acatataaga tataactaac gattaaatta ttacttatcc aataaaaaat gactgccgga
    34441 aaaaaggtca agtagggata aaaataattc actccgctcc cataatgatt aaacgtcgta
    34501 aaattgattg gaccagaaaa aattgaagat aaccctttga ctctagcaaa atggaaggca
    34561 atatcccccg atgtagaaat agtatgaaca tttctaaaat aattgattga ataaagtcca
    34621 gcaataattg ctaggagaat ggacaatatt atttttttct ttacttgcat tacttaatta
    34681 tatactcctt tcatggattg ctttatctag tataacttaa attttaaata aatcgttatt
    34741 tatttttact ctcacttaaa aaagcactaa aattataaga gagggaggta atggtctgaa
    34801 caccaagaat tgttccaaca tcgaattgct taactaatga gttcaaagct taattatgtg
    34861 gctcttatat ttaccgagcg tggattgcga tctctttagc gaaacaaaca tctttcataa
    34921 aaaaaacagc catatccgct ttcttggata tgactattta ctactacttt actatctttc
    34981 attcttcttg tctcacttat gataaggcgc tgggtatcat gagtgaatga tattgctgaa
    35041 tacaaatagt ccgaccacat taagttgatc ggacttcata agcattttat tgactaatac
    35101 ttttttgctt aagaattgat tctaacatct tttgctaaag ttaagcatcc tatagttcct
    35161 gcattatctg ctaacttagg tgtgacaatg tattcttcga tatttggcgt atcgacatag
    35221 ccgttaatta gttctaaaaa acgtctttgt actttctttt tcagatgttc ttgttgcatg
    35281 acaccaccac caaaaattat aatatctggt gaaagcatca gggtcgtatt acaggcacat
    35341 tgtgcaatat aatccgcctc gatttcccag aacggatctt ctatagaaag atcttgtccc
    35401 ttggttcccg tacgcatctc gatagcgggt ccagatgcca acccttcgag acaatcatga
    35461 tgaaaaggac aagtaccaga gaaagtatct cctgaatgtc tcttaactaa cgtatgcccc
    35521 atttctggat ggctaaaccc ttcaacaaaa atcccatctt gtatagctcc tccaccaatt
    35581 ccagttccaa cagtgtaata aacaacactg gataaacctt ttccatgacc tgaaacatac
    35641 tccccgtaac aagcagcatt cacatcggtt gtccaggcaa ttggaatttc gaaatgctta
    35701 ttcaacgttc caacaaaatc aaaatttttc caggcagttt ttggagtaga ggtgatatgt
    35761 ccataggtct ttgattcacg atggatatca atcggtccaa atgaccctac tccaatgctt
    35821 tctaattgtt ctttatagtg attaaagaaa tcaattacca aactcattgt ttcattcggt
    35881 gtagttgtag gaaaactcac tctttctaaa acctttaaat catcattgcc aatggcacaa
    35941 acaaatttag tcccacctgc ttctattgag ccatacattt cttactccac cgtcctttta
    36001 tctaggaatt cgttcttccc tgtctaatag tttttcgaaa aactcatgtg gttccttttg
    36061 gtaccaatag gcaacagaag aaatatcgtc ttgacgctca taaagcttga tgtcatcatt
    36121 tcctagggtt tggaaagtaa cacgtaaatt ttctttgaaa ctaatggggt cgggtaaatg
    36181 ccagcggtac aacccatgca tcggtaaagc atcatcgcca aaaccatgaa ctggatttgg
    36241 attaggtttt ccagcactaa aataatcccg ggtgtgatct cttttagatt gaaaaggata
    36301 gcccataaat agcgtattaa aattttgagc atatgccctt tctccaaagt tctttttatg
    36361 gaaagcccaa gcgccgccaa aataatcttc tgctcctgta gatgttattg tagggaaatc
    36421 ttcatctcca tctatataaa acttaaattc tccttccccc caccagtatc tttccaatgc
    36481 gcacaaagca aagtaggttc ctacataata cccagtgcct ttgattccat caataagggt
    36541 gtaatctttt ccttttgtcg tgatgggttc acggttccat ttagcatgaa aatacatcgc
    36601 atcatccggt acttcagtca atgcataatt aaatgtataa aaaatgtgct taataaactc
    36661 aggatgttca ttcgttaaag taatcttagc tttttttcga aatggcatct caaaatagca
    36721 attcatccct gatgttggat tcactacgat tggtaaggaa ttcacttcgc atcttgtccc
    36781 aaacccatta caaaaaaaat ctcctaatgg actttctaca gaaggagtgg attcatcatc
    36841 ccaataaata cgcaaaatta aatcacgtaa gacgaaactc ccagcgattg ttttgtctgt
    36901 cactgtcatc caaaaatgac gtaaaattcc agggccttcc atttctgcta aagtaattgt
    36961 ctctccagca tttatcggaa cagaaggtga cccttttctc ccaattccta gatgactcgc
    37021 tgtcattgcc gctttccctt tttcaccatt tttattttct ggtgaaatag ttctactttc
    37081 ctctcccttg tggagccata tattatttaa acaattcatt ttattcgtcc tctatccttt
    37141 cttcactttt ttgattattc atatagatga tttgttgttg tgcctccgca ataattgctc
    37201 caatatctaa attatcattt cctaactgca aagcgattga cattgccaag ggaagattca
    37261 ttcctgcaat tacgtgcact ctgtcagaaa tataggtaaa aaacttttga tttacacttc
    37321 ctccagccat gtcagttaaa acaattattt cgtctgtagt atccactaac tctttaattt
    37381 gttcatctat atcatcaatt tcttcgacat atgcacataa gctactaatt tgattatgca
    37441 tacctgttaa aaactctagt gtctctttaa aaccctcagc tagtattgaa tggctagcaa
    37501 tgatcaatct tctcataatt caaatccttt tcctttacgc taatattcca aatgcagcca
    37561 taaccatcgc aaaaacaata acgagtagaa tcaaaatggt catactgatt tttttcttac
    37621 tgagtagata atatgctgca gcagtaataa ttactggaat taatgttggt aaaattttgt
    37681 ccaacattgg ttggattttc atactaactt caccaaaagt gaatttaagt ggcgttgaaa
    37741 cactgataac tgaagcaatt aatgatccaa ctactgttag ccccaatact gaagcagctt
    37801 cagttaaata attaatcttt tcaccgtatt cagtaacaat cttcattccc tgcttataac
    37861 caaattcaaa gcctctcatt cggaaaaaga agattaccat gattaaaatg aaccataaaa
    37921 agattcctac aggattattt tctaaagcca tataagccgc aattgatccc atgatggttg
    37981 ggatcatcgt catcaaaaga gagtccccaa taccagcaat tggacccatc gtaccaactt
    38041 taaagtcatt aatcgcatct tttgcttcta atccttgctt ttcctccatt gcaacacaag
    38101 cacctaaaat aatggctgcc agccaaggct gtgtattgta atactgaaag tgattattta
    38161 aagcttcgat gtattcatca tcatctttgt ataattttct taaaataggt gacaatgcgt
    38221 aagtcacaga tgcaccttgc tgtgtttgat aattaaaggt acaaactgac atcaaccaac
    38281 gatagttcgc tttcctaaga tcctttttcg ttacttgata tttttcgctc attttattca
    38341 tcctccccaa taaacccaac atcagaagct cccgcagaag cagtgatttg tgcatctttt
    38401 tcagctctat gatacacatt aaatgctaaa gcgaatccta tgatcgccgc tccaataata
    38461 ggtaccttca aaaaagcggc taagaaaaat ccaacaagta aataactaat gtatcgacct
    38521 gtaggcataa agcgtaacaa catcgcaata ccaacagcag gcaacatttt tccggctaat
    38581 gttagcccat ccataaacca attaggtaaa acatccaaaa cgtaattaac agcagactgt
    38641 cctaaagtaa cggcaataaa tactggaatt gctgcagata aaccaaagaa aattggcccc
    38701 aaccacaaaa cactattcat tttcttaaat tctcgtttat tcgcatattc ctgtgatttg
    38761 cgaacaacaa aaccatttaa gattttgaca attacatcta gttgcacccc taacatacct
    38821 acaggtagtc ctattgctaa tccggcttct actccaagct ggctaccaaa tgccgttgca
    38881 ataattgcgc acgttccata gtccggtaca gatgagccac caaaaccggc aactcctaat
    38941 gacatcaatt gaactgtacc gccaactatc aaacctgttt ttatatcacc catcaaaatt
    39001 cctgtgaata atccccaaaa aactgcgtaa tttacaaaga tcatcggaat cccataataa
    39061 tcaacatgtt taataaatgc taatatagtc agcataataa tttgaaaaat agtaaaatgc
    39121 attatcctta ctcctttatc ctaattttga ttgaatttcc tgtgccttat ccgccggaac
    39181 aaattgtgta acaatttcta caccagaatc caacaatttt ttatacgtgc tgatattttc
    39241 atctgatgca taggctctag aagatagctg taatgcatta tctttaacaa cagtaccgcc
    39301 aacaattagt cggtcaattc taataccgtt atctactaaa tgaaggattg tttccggctc
    39361 tttcacgatg acaaatactt tttcctgatc gtatttcccc tttttaaaat tcgcaatcgc
    39421 tgtttcttca gtgataattg atacagccat tccagcaggt ctagctaaac gcatacttga
    39481 tttttggatg tcgttatttg cgacagaatc atcgatcacc atcactctgt ttgctttaga
    39541 cattggtgcc cattgcgtga caataatccc atgcaataaa cggttatcaa ctctcgacat
    39601 aacaatactc ataaatactt cctcctttat ctttcatttt cttacaagca attaccatgc
    39661 caaaacaaaa agaacttgat aaatcaagct ctttttacat aatcataaac gtatttaacc
    39721 tcatgaatcg ggatgtcgac actataataa tgttcaacga cactgaaaga ttgcttaaaa
    39781 tgtgtctgaa atttttgaaa ttcctcactc gtttcattga aataaatttc atcttccaaa
    39841 gtattttgtt ttactaatcg ttcaattaag caactaatgt gaacatataa tccgaaacac
    39901 gtattattat caaatgaaat tcctagatc
//
LOCUS       DAZKBH010000014        38360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000014 DAZKBH010000000
VERSION     DAZKBH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.014"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            308..628
                     /locus_tag="ACG8OO_000739"
     CDS             308..628
                     /locus_tag="ACG8OO_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFP9044666.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            782..1687
                     /locus_tag="ACG8OO_000740"
     CDS             782..1687
                     /locus_tag="ACG8OO_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044667.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1684..2457
                     /locus_tag="ACG8OO_000741"
     CDS             1684..2457
                     /locus_tag="ACG8OO_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9044668.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2638..4062)
                     /locus_tag="ACG8OO_000742"
     CDS             complement(2638..4062)
                     /locus_tag="ACG8OO_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFP9044669.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4273..4719
                     /gene="nrdI"
                     /locus_tag="ACG8OO_000743"
     CDS             4273..4719
                     /gene="nrdI"
                     /locus_tag="ACG8OO_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9044670.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4784..6766)
                     /locus_tag="ACG8OO_000744"
     CDS             complement(4784..6766)
                     /locus_tag="ACG8OO_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP9044671.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6780..7676)
                     /gene="murQ"
                     /locus_tag="ACG8OO_000745"
     CDS             complement(6780..7676)
                     /gene="murQ"
                     /locus_tag="ACG8OO_000745"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9044672.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7679..8740)
                     /locus_tag="ACG8OO_000746"
     CDS             complement(7679..8740)
                     /locus_tag="ACG8OO_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP9044673.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8951..9478)
                     /locus_tag="ACG8OO_000747"
     CDS             complement(8951..9478)
                     /locus_tag="ACG8OO_000747"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9044674.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9545..11047)
                     /gene="eat(A)"
                     /locus_tag="ACG8OO_000748"
     CDS             complement(9545..11047)
                     /gene="eat(A)"
                     /locus_tag="ACG8OO_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9044675.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11305..12426)
                     /locus_tag="ACG8OO_000749"
     CDS             complement(11305..12426)
                     /locus_tag="ACG8OO_000749"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9044676.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12552..13493
                     /locus_tag="ACG8OO_000750"
     CDS             12552..13493
                     /locus_tag="ACG8OO_000750"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9044677.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13643..14539)
                     /locus_tag="ACG8OO_000751"
     CDS             complement(13643..14539)
                     /locus_tag="ACG8OO_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9044678.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14808..16148
                     /locus_tag="ACG8OO_000752"
     CDS             14808..16148
                     /locus_tag="ACG8OO_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9044679.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16366..16809
                     /locus_tag="ACG8OO_000753"
     CDS             16366..16809
                     /locus_tag="ACG8OO_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFP9044680.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17313..18731)
                     /gene="nhaC"
                     /locus_tag="ACG8OO_000754"
     CDS             complement(17313..18731)
                     /gene="nhaC"
                     /locus_tag="ACG8OO_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9044681.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19134..20357)
                     /locus_tag="ACG8OO_000755"
     CDS             complement(19134..20357)
                     /locus_tag="ACG8OO_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9044682.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20388..21227)
                     /locus_tag="ACG8OO_000756"
     CDS             complement(20388..21227)
                     /locus_tag="ACG8OO_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9044683.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21244..21924)
                     /locus_tag="ACG8OO_000757"
     CDS             complement(21244..21924)
                     /locus_tag="ACG8OO_000757"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9044684.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21926..22999)
                     /locus_tag="ACG8OO_000758"
     CDS             complement(21926..22999)
                     /locus_tag="ACG8OO_000758"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9044685.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPEELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23055..23293)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23336..23569)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23646..24317)
                     /locus_tag="ACG8OO_000759"
     CDS             complement(23646..24317)
                     /locus_tag="ACG8OO_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9044686.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24404..27052)
                     /gene="mgtA"
                     /locus_tag="ACG8OO_000760"
     CDS             complement(24404..27052)
                     /gene="mgtA"
                     /locus_tag="ACG8OO_000760"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9044687.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27394..27568)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27741..28178
                     /gene="ndk"
                     /locus_tag="ACG8OO_000761"
     CDS             27741..28178
                     /gene="ndk"
                     /locus_tag="ACG8OO_000761"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9044688.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28200..29066)
                     /locus_tag="ACG8OO_000762"
     CDS             complement(28200..29066)
                     /locus_tag="ACG8OO_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9044689.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29226..31319)
                     /locus_tag="ACG8OO_000763"
     CDS             complement(29226..31319)
                     /locus_tag="ACG8OO_000763"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9044690.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32068..32691)
                     /locus_tag="ACG8OO_000764"
     CDS             complement(32068..32691)
                     /locus_tag="ACG8OO_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044691.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32850..34247)
                     /locus_tag="ACG8OO_000765"
     CDS             complement(32850..34247)
                     /locus_tag="ACG8OO_000765"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9044692.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34279..35250)
                     /locus_tag="ACG8OO_000766"
     CDS             complement(34279..35250)
                     /locus_tag="ACG8OO_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9044693.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35624..36049
                     /locus_tag="ACG8OO_000767"
     CDS             35624..36049
                     /locus_tag="ACG8OO_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044694.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36537..37238)
                     /locus_tag="ACG8OO_000768"
     CDS             complement(36537..37238)
                     /locus_tag="ACG8OO_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9044695.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37254..37694)
                     /locus_tag="ACG8OO_000769"
     CDS             complement(37254..37694)
                     /locus_tag="ACG8OO_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9044696.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37694..38269)
                     /locus_tag="ACG8OO_000770"
     CDS             complement(37694..38269)
                     /locus_tag="ACG8OO_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFP9044697.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT        11082 a         8008 c         6686 g        12584 t
ORIGIN      
        1 tgcccgataa tatatagaaa taaggtctag ttactgtttt ttttaaatga ttaataaata
       61 acaacttctt tctatgaaat tgcaaagcat tcatcttctt ttccgctaac gatatatccg
      121 tcagtgcttg gtacacttta ctaaaaagta tgagattgcc gatatctgga taggtgtgct
      181 atactacaga aaaagatata caatctgcca ccgggtagga taaagaggat gatcattcca
      241 ttaggtcttt gaagggatga ttttcctctt tttgtgtatt tttttatagt taaggaggaa
      301 tgtaagtatg gcatggatgg aattaatcat agcaggaatt ttggaagtat tctggtcaac
      361 gatgatgaaa tggagtgatg gtttttctaa aatcaattat tctttttaca cagtgattgg
      421 catgattgct agtttttact ttttgtcaaa ggcaatcaaa agtttgccga tgagtctctc
      481 ttatcctatc tggacaggga ttggcgcagt tggttcggtc ataattggtg tggtcttgtt
      541 tcatgataag ctgaatcttt ccacttggtt ctttgtgggg atgctactca tcagtatcat
      601 tggcatcaag attactagcg gccactagac acttggaata agtactgatc agaaaaaata
      661 agccttttta tttaatattc aattttttta tgaaaaaata agaaataagt ccaatgacct
      721 agcgacttct ccactcatca ggtgtatgct tgtactatct atagaaaagg agtgaatatt
      781 tttgaaaagt ctttcggtcc aagaattatc caagaacttt ggtcacaaac aagccctcag
      841 cgatgtcagt tttagcttat cccctggcaa aatcgttgga ttagtaggtc ctaatggcgc
      901 tggaaaaaca acgatcatga aagctatttt aggactgatc cattactcag atggaacaat
      961 ctgtattggt gaaaatgaga tttctccctc tagtcataaa gggttggaac aagtaggagc
     1021 attgatcgaa tacccgggta tctatccatt cttaacaggc tatgatcatc tgaagttatt
     1081 ctctgaaacc aacgatgtat ctgctatcga cactatcgtg aatcagctca agatggaaaa
     1141 gtacattcac aaaaaagcga aatcctactc gttgggaatg aaacaaaaat tgggaattgc
     1201 tcttgcctta ctgaaccatc cgtctatcgt tattttagat gagccgatga acggtttgga
     1261 tccacaagca acaagagatg tgcgaaaaat cattaccgac ttggctactc agggcacctc
     1321 tttcttgatc tccagtcact tactcagcga gcttgaaaaa atggtcgatg agctgatcct
     1381 gatcaatcaa ggaaagatca tcaaacaatg tacaatggct gaattgaacc aaacagcgca
     1441 agatttcatc gttatctcta caacaaatga tctagctgct cgaacgatct tgacagaagc
     1501 tggatatact ttggaagatc ctgataaaat caagctttta aaagaaaacg aagaattatt
     1561 aggaaccgtg atccagctat tgtctgcaca aaatatccat gtgacagacg tccagcatct
     1621 gacgaatgat ttggaatctt ctctattgga aattctggat tcagtaaagg gggaagaaga
     1681 aaaatgacat tgcttgtaaa acaagaatta tttaaattga tcaagaaaaa gtcaactgct
     1741 gttttatctg ttctattagt tgttttgctc ataggaacag cacttctagc caaaaaatac
     1801 acgacgatca ttgatccagt tgaaatgact gctcagcttt ttagtgccac atcttggatt
     1861 gtctttatca tgattgcagc tgcaagcacg atcatttcga tggaagcaca atatggtaca
     1921 ttgaaaaatc tcctttatcg aaagtattct cgaggagaaa tattagtaag caaatggatc
     1981 acattagtta tttattctgt ttatttatac cttttagcta ttattgtcac tgtgttgatg
     2041 aaactgattc ttttcccttc gatttccttc acggagaagg tttctactgg tcaaacactg
     2101 atccaaagct tgttcactta tactttgggt agttatatcg gtttatggct gattttaagt
     2161 ttagtgttga tgattgcttg ctttatcaat agttccggcg catccatctc agcaggaatc
     2221 gtcttttatt ttgcttcgtc aatcatatct ggtatcctta ttgcactcat ccaaaaatgg
     2281 gagtggatca aatggaatcc gatcagtatg ttgaatctgc aaaaccaaat tggaaacgaa
     2341 gagatcatga agcaattgac tcatctttct actaatcaaa tgctattcgg taatttagct
     2401 tacatcgttt tgttcttagc attaggatat ctcgtattta agcgaaaaaa tgtatgatga
     2461 ctcatcaaaa aagaggctgt gaaagagacg ttcagcctta ttgccgattg gctgtctctt
     2521 aaacaccgtt tattctgaaa aaggagctgt gacaacattt gtgtcacagc tcctttccta
     2581 gtatgttttt gaataaacga caggctcaca acctctcttt atacttcttt tatacttcta
     2641 tacgtgaagc atcccagtgg caaataaaac cattgcaatc actccgacca tcgtcacacc
     2701 tataagtaaa atcttatcta agtggatatg ttgtacaggc tcgttttgct ccttacgtgc
     2761 tttgaggtaa aaataaatgc ctggcaagaa aagaacaaga acaagcatta cttcattaaa
     2821 ggctacgact agcatagccc atacttgaaa ggcacttgct aaaagcccta ctaaaaattg
     2881 gacccattct ttccgctgat aagaaagttt catttggtac aatcctaaga aaagccacgt
     2941 gaagaaaata gctgctgtac caagtgtgta agcaaagtta taagcactgt cagtgaataa
     3001 taatgaaagt aaaaagacat tcgtacataa cgtcgtcaat acgatagcat aagttggtgc
     3061 cccatttttg ttgagttttc cccatgcttt aggcaaaagt ccgtcttcag ccatgtttcc
     3121 cattgtttca atcggtaaga tcgtccatga gagccaaaca ccaactaaag aaatgatcaa
     3181 accgccgttg atcacgactg ctccccattt accaaccatc atttcaaaca catagcccat
     3241 agcaggctgc gccttgatcg tttgcagctg attcatcgtc attgtcccat atggaagcat
     3301 ggaaaccaag acgtaaataa taatcaagcc gaatatccct agaatcgttg ctttcccaac
     3361 atctgattgt ttttttgctc gtgaacctac gacagaggct ccttcaatac caacaaatac
     3421 ccaaagcatc accatgatgc aactcaatac ttgactggaa aacgggatcg gctgtccatt
     3481 cgcattcaat ataatgtcgc tagaaaagtt atcaatgaat tgttggatcg aaaacgtgct
     3541 gatagctact acaataaata aaaataatgg cactaattta cagaatgtga cgatagcatt
     3601 gacgaaacta gcattttcga ctccccgatt cactagccat gcatatcccc ataaaaagac
     3661 tgtactcatc acgatcgatg ccagattttg cccaccttca aatactggga agaaatatcc
     3721 taatgcgctc atcaacaatg ttgcaaaagc tacgttgcct aaccagcatg tgagccagta
     3781 accccaaccg ctgataaaac cagcgaaacg gccaaaacct tcttctgcgt aactaaagat
     3841 cccgcctttt aaatctggac gttttttatt gatattattg atcgacaagg agagcatcaa
     3901 cattgctgtt cctacgagta cccaactgaa aatagctggg ccgccagccg atccggagat
     3961 gtccgtcata ataccaaata cgccacctcc aatcgctgaa ccaattacta aggcagcaag
     4021 tggaattaaa ccaattcctt ttttctgttg ctctgtatcc atgtaagcat cctccaaaat
     4081 aaaaccgctt ttatcaaaaa gtataacaaa ccttttttat ttttacttaa ttaataaaaa
     4141 ctttaataga aatataattc taccggcttt tttctcatta tacaagcaaa gcgcctagtc
     4201 cctttccaaa ttacaccacc aaaaaactta aaaaagatgc tatactaaaa ggaaagaatt
     4261 ggaggatgat ccatgaacat tttatatata tcgatttcag gaaatacgcg tgcctttgcc
     4321 aaacatctag cagaatatgc tgaaaaaatg catgcagaag atcctgtcaa tcctgaagtt
     4381 actttaaaag aaatccatga aaatagtgat tttacgaaag aaacagaacc attttttact
     4441 ttcgttccta cttatttaga cggcggaaat ggtttagata acggtgatac agaaatcttg
     4501 accgaaacga tgcgtgaata tctggaacat gaagacaatc ataaactttg tcttggtgta
     4561 gtcggtagcg gaaataaaaa cttcaataat cagtattgtc taacagccaa acaatatgca
     4621 caaacatttg gctttccttt cttggcagat tatgaattac ggggaacacc atctgatgtg
     4681 acacgaattt atcagatttt agcagaaaac aataaataat aaaaaagaag aagctgaaaa
     4741 atagatttca gtttcttctt tcgtttctat tgtttctatc atattaagct ttgacatcca
     4801 tcacaggtgc ttttgctttt gctttacctg ttttttccaa gacaaagttt tttacacgat
     4861 ccatattggt taccaagacg atcatggatg tttctttgcc tgctgctttg atcgcttcta
     4921 agtcagcttc tgctacaagc gtatcaggtg tcacttgctg cccttctgtt actttcacat
     4981 caaatggcgt accgcctaag tcaactgtgt tgattcccat atgaagcaag atttctaaac
     5041 catttggcgt tgtgatccca atagcgtgtt tcgttgggaa gacagagctg actaccccag
     5101 taactggaga atagatatgt ccgttttctg gttctacaaa gtagccttca cccatcatct
     5161 tttgtgcaaa aactggatca cttgctttat ctaaagaaac aactgttccg tcagcaactg
     5221 atgagaggat agtcacactt tcagctgaag tttgaggtac tactttttca ggcacttcat
     5281 ttactgtggc tgtttcagtc gctaattctt ctttttctaa acggtcttta ggaataccga
     5341 agaagaatgt agcaacgaag ccaccagcat aagctgccaa taatccaagg atgtagccta
     5401 accatttacc gtcagagatc aatgggatca aagcggctcc tgaaggtcca atagcgatag
     5461 ccccgacatt tccgatcatt ccgatcactg ccccgccgat acctccaccg atacaagcag
     5521 taataaatgg gcgtcctaga ggtaatgtta ctccgtaaat caatggttcg ccgattccta
     5581 ggatcccgac tggtaaagct cctttgatca tttctgttaa ttctttatct tttttgcagc
     5641 gaatccataa tgccaatgct gcacccactt gtcctgcacc agccatcgca agaattggaa
     5701 gcaatagcgt acgaccagtt tcatcaatca tttgcacatg gattggagtc aaaatttgat
     5761 gcagtccgaa cataaccatc ggcaagaata atgtgcctag aacgaagccg gcaaatgctc
     5821 caccaacgcc aagtacccag ttgatcccac cgaccatgcc atcagaaatg aaacctgcta
     5881 aaggcatgat gaagaaaatt tcaatcagac cgatggcaat caatgaaaga gttggtgtaa
     5941 cgatgatatc taccgcatca ggtactactt tatgcagttt tttctcaacg attgacaata
     6001 gccaaacagc gaagatgaca cctaggatcc caccttgtcc agctgcaaga gcttcaccag
     6061 taaataagtt tttcagtggt gcttccggat tcatccctgt cagcatcaca acggccccga
     6121 taactccacc aagagttggt gttgcaccaa aaacttgtgc agcattgata cctgtataga
     6181 taactaggta gctaaacatc ccgtttttca agatgttcat cacatcgata tattgttgcc
     6241 atgttgctgc atcaagtgtt ccggctgtga ctaagtttgc taaaactgcg gcaatccctg
     6301 ccacgatccc agtaccgaca aatgcaggaa tcattggcac aaagatatta gaaatgtctt
     6361 ttaataccga tttgatttta gatggttttt gtttcgattt ttgtgccgct ttcatttctt
     6421 gtgcccgttc gtttacacgg tcttttccag atgctgatga atttgctgta gctgtactta
     6481 cagaagaaag tttttctcct aatttcacgc ctgccaaatc aaccatttgc tgtgctactt
     6541 tgttcacttt cccaggtccg atgatgactt gaagctgttc atcttctact acaccaagca
     6601 ctcctggaat cgcttttaat tcagagacat ttacaagatc atcattgatg atcttcatac
     6661 gcacacgagt catgcagtgg acaatgcttt ctacattgcc catgccgcct gcccctttat
     6721 aaatttgttt tgccagttct tccaaagtca gttcgttttt tgccatggtc ttttccccct
     6781 cataaggtcc cttttacaaa tccgtttgct tcagctaatt ttgcttgagc ttcctcttta
     6841 tcactatttg tcaaaatcat gacgatagcc agcttaacat tttgatctgc ttcttcaaag
     6901 tacttttcag ctgtttcgta atcagcttct gtcgcttgca tgatgattcg ttttgagcgt
     6961 tcgattagtt tttcatttgt tggtttgaca tcgaccatca agttattgaa tactttacct
     7021 ataccaatca tactgatagt agaaatcata ttcaagataa gtttttgtgc tgttcctgac
     7081 ttcaatcgag tagagcctgt caaaaattct ggacctgcat cgacttcgat tgggaaatcc
     7141 gcgtgtttac tgatttcggc ttgtttattg caagaaatcg ttcctgtgac agcaccgatc
     7201 gattttgcgt aatctaatcc accgatcaca tatggtgtac gaccgctagc tgcgatacca
     7261 ataactatat cattttttgt caaattcagc ttgattagat cttcttgccc tagctcataa
     7321 gaatcttcgg ccccttcgac agctacagtc attgcttctt ctccaccagc aattagacct
     7381 atgaccattt ccggttctac accgaatgtc ggtacgcatt cagcagcatc taacacacct
     7441 agtcttccac ttgttccggc tcccatataa atcagacgtc cacctttttt aaaggactca
     7501 atcgtgttag caatcactgg ttcgatactt gtcagttctt tttcaaccgc agcggccact
     7561 ttctgatctt ctttgttcat tagttgaacg gcttgagaaa tactcatctc atctaatcca
     7621 aaagtttctt ggtttctgcg ttccgttgtt aggttctcta aattgatttt gctcattttt
     7681 atcttccttc ttttcttata aattgatagt tcataccagc tttgatttgg ttgatcaatg
     7741 gtagctcttc tttgataact ttagctacta cattcacttt ttcatccgct ggcaaatcat
     7801 atttggttaa ttggatctca cccatatagc gcaaatactt ttcgttatct aaagtaatag
     7861 accctttcat acgcgtagct gtatttcttg ctgggatagg cggaatctct cgaaaacgtg
     7921 catctacgct tcgtatgaca tctcttgcat cgtcttgccg attcgtatgc ttccctaata
     7981 tatattcgta aaattcttca tctacaggtt ccgtatggag caagactgtc tgttcttttt
     8041 ggaaagaagc aaattgctcc tgaacgtttt ctgacagacc tgcatctccg atgtaaacaa
     8101 tatccgtgtt acttgctaat agatctagag cgcctgcaag aggatggatc cctcgatgtt
     8161 cttccaaagt tggcaatcct tgatatagtg ggcctctcaa ctctgtatct cctggtacaa
     8221 aaccttggac aaggagacct tgaattttga gccattgatt ttttttctga taccattctt
     8281 tgtccagccc tgtttcggga cgtggataat aattatgcca agcttcaatc tgtgagaaat
     8341 cagcttctgc ttctttcaat tcatcgatat ctttaggcgt gatcgtgcta gcattcaaac
     8401 tgattttcaa ttgatgtgac cagtcggcaa tctgctgatt ggaaatatga taatccattc
     8461 ttaaacctgt tacaccaatt gctttcaaag gcgttagatc ttctaatgaa aaacccgctc
     8521 gttccaatgc ctctcctgaa atatcgacca tcaattctag tttattcttt ttcgcttcgc
     8581 ttcctagttc gatcaatcgc ttttgatatg ccgaaacatc atcttctggt atatgcatgg
     8641 aagtgaaaat accgacaaag ccattggatg ccatttggcg gatatactgt ttcttctctt
     8701 ctgacaaaga atcgttcata aatattgaaa agccaaacat attatccctc tttttctgta
     8761 tccgtcttca tttctatgac tagagtatat atctaaagaa acaaaatttc aatactttta
     8821 ttaaaataat taaatttcat ttctaaaggt tgagtttata ttatcaaaag aattgtcata
     8881 acctaaaaaa ggcactgcta agcaaaagca gtgccttgaa atattttatt taaataacac
     8941 aaaaacataa ttactcataa gaaaccaatt ctgtattatc taccatgaat gtaaagaacc
     9001 cctcattagg catcggatga aaataagcat tgatcactct tatcaaggca ttatggcaaa
     9061 ctaacagata gacattttct tcgtcttcga tgcattcttt caataaagga acgatccgtg
     9121 catacacatc taagactgac tccccatttg ggaatcgtac agcgaacgct aaacgtgctt
     9181 tttgaaagtt ctcatctttg cttggcagtc catcataatc cccaaaatcc atttctacca
     9241 atcgttcatc aacggtcagc ggcaagctga gacgatcagc aactgcttgt gctgtatctc
     9301 ttgctcgttg aagaggggaa tggatgattt tcgtgattgg aacaggcaaa tctactacct
     9361 tttctgctag tttctgtgcc tgcatctgtc ctttttcagt cagcggcaca tctgcccgtc
     9421 cgcaaactaa tccttgtgcg ttccaagttg tttctccgtg tctcgttaca taaagcatgt
     9481 gattctctcc ttcgttggca aagttcccct taaccatacc ttgttgttct tgagattaca
     9541 agggttagat tctctcaaga gaaataatct cagtagatac tttatccagg aaggtttgat
     9601 catgttccac tagtaacatg gcaggtttca cgttcaagat cagttgttct aattgttctt
     9661 gattgaagac atccaaataa ttcaatggtt catcccatgt atatagttca gctggctgtg
     9721 ataaagattt agccaattcc acttttttcc gttggcccat actcatctgc tcgatcttgt
     9781 tatgaaaaac atctctttcc atcccaagct ttcggaggtt gttcaaaaat gcttggtagt
     9841 ctacttggtt tttctctgca aattccgcca acgttcctcg attgtcttca taattttggc
     9901 ttgcataact aatgctcaga tgttttggct ggtatttttc tcctataacc ttgccgttga
     9961 atgcccccag aagataatgg ataatggatg actttcctgc accgttagga cctgaaatcg
    10021 ccacccgctg atgaggctcg attgtaaaat gaattggctc gaataacaga ttttcatacc
    10081 ctaattgaag atcttctacg cttaaaagtc gcttatggtg agacgcttgg ctattcatcg
    10141 tcaaagaatc gatatattcg atatctttta atagtttttc tttctcgctg atctgggtct
    10201 ccatccgatt cacgattgct ttggatcgtt tcatcgtccg tgcagcatca gcacccactg
    10261 ctcctttatt cactcgtcta gattcagtat cgatgaatcc gacttgtttc tttgttttat
    10321 ctccttctcg ggaacgagac cattcggctt tttcagctgc tgttttcttt agcctactga
    10381 cttctttttt caatttttca ttttgagcca tttcaaattc atcacgaagt tttttctgtt
    10441 cttcatagat agagaaattc ccttgataaa gttccagttg acttttttcg attgctaaaa
    10501 catggtccac tacttcatcg ataaatcccc ggtcatggct gaccacgatg aagccttgtt
    10561 ttttcttttt caaataagcc gctacttgtt ttctaccaga gatatccaaa tgattcgttg
    10621 gttcatcgat taacgggaaa tgagtgtcat ccacaaataa aagtgccagt aggacttttg
    10681 tcttctctcc ccccgatagt gtgctgaatt ctctccataa gatttcagga tctgtttgca
    10741 tcaattggag ctctctttcg atttcccata tctcaaaatc cgtaatatca tttaacacgt
    10801 aataggttaa acgttcttta tcttttgtct gttgcgggaa ataggcaaat tcttgctgat
    10861 ggattacttg cccttgataa ggtagtttgt tttgtagaat attcagtaaa gttgtctttc
    10921 ctcgaccgtt tcgtccgata agtcctagtt tccattgtgt gtcaaaattt agatttgctt
    10981 gttcaaataa taatgtgcct tggctgtcgt agccgaatgt cagatttttt atttcgattt
    11041 tagacatttt ccatcacctc gtaaaaaaag accagtccta ttttcagaac tggctaaaga
    11101 ggatgcaagg gttggataca cacaaaaaga cccatacatc gaaaaaacca agccaatccc
    11161 gaaaatctgc acactgcttc cttgtctcca caaggtacag tctgaatgca gctttcatca
    11221 atcgacttaa tttttcaatg cacggatccc tcactttgtc ctatatttga aattacgata
    11281 gcataaagaa aatgattttg tcaactactt cgggaggatg gtgaaaaatc tgctaagtgt
    11341 ctgtcctaag atttttgttt tttctaatgt ttgcctgtgt tccattgctt cttctaaacc
    11401 tattggaccc aaatgaatac ttaagacaat tgggagaaaa tcagaaagtc cttctagctc
    11461 ttggtctcta cttccgcaaa tagcgattat tggttttcga tatttttttg caagcactgc
    11521 tacaccgtat ggaacttttc cgtggatcgt ttgctgatcg atccgccctt ctcctgtaat
    11581 gatgacatct gcttgttcga tctttttttc caatccagtg atttggctga ctaaatcgaa
    11641 accagaaacc atcttcgctc ccaacaaaaa aagggctcca ccaataccgc ctgctgctcc
    11701 acttcctgga atatcatcta aatccacctc ccagaaatct tttatttgct gagcaatctt
    11761 tcctgcttgt ttatctaaga aagctagcgt cgtatctatt cctccttttt gtcttccaaa
    11821 ttgccaagca gctccttgtt gacctgtata tggattggta acatctgcag cgataatcaa
    11881 ttttacttta gaaacaagaa cgtttcttcg atcaaaatga agttttttag tagataataa
    11941 aggatttcct tctgtgtacc cttctaaaga agcacccaag gattggagta atcctagacc
    12001 accatcgcta cacccgcttc ctcctaaagt gacaataatt tgttctactt cttgaagcag
    12061 tgcatcttta ataagttctc ctaaggcata actggaagca agtcggatag tttgatccgt
    12121 tggtttatca agtagatcta accccaatgc ttgtgcagat tcgatgactg cggtgttttt
    12181 tccttgccaa gttgtcaata aatagctgat ttttcgctga cggaataaga gatccatccc
    12241 atcaatggta cgccatgttc ccctgagcgc gtaatagatt gcttctgcag tattttctcc
    12301 gccatctgca atcggcacat tgatttttct tcctttacga tcttcccagc catttagaac
    12361 tgcctgattt agttcaaatg aagaagcact tcctttaaat gaatcaattg ccgccaaaat
    12421 atccatctgt atccctcact ttcttcttaa gtaagacact ttttttcatt tttttcaaac
    12481 aaaacaatgc tattttcttc catttttcta tatactaaaa gggaactcat tgaataaagg
    12541 agagaaaaaa catgaaaatc gcaatagctg gggccggagc aatgggcagc cgaatcggat
    12601 tgatgctaca tcaaagcgga aatgaggtcc tcttgatcga tcgttggcct gctcacattg
    12661 aagccattcg tacaaatggg ttgattgctg actttaacgg taaagaagtg gtcgcaaaac
    12721 tgccaatcta ttcaccagaa gaaatcatcg aaagcaacga acacgtggac ttgatcgtag
    12781 cattaacaaa agccaatcaa ttagatgata tgttttgttc gatccaatcc attattactg
    12841 acaatactta tgttttgtgt ttactaaatg gattaggtca tgaagatgta ctggaaaaat
    12901 atgtgccaaa gaaaaatatc ttatttggga tcacgatgtg gacagctggt ctagctggtc
    12961 ccggaaaagt cacattgtta ggtgatggag aaatcgaact agagaacttg gaaccagaag
    13021 gcgaagcatt tacaaaaaaa gttgtagaag tctttcaaga agcaaattta aatccgatct
    13081 atagccataa cgttcgttat tccatttggc gaaaagcttg tgtaaatgga acattgaatg
    13141 gtttatgcac gattttggat tgtaatattg ctgaactagg cgcgcaaaaa gcggcagaga
    13201 gcatggttcg aacaatcgta tctgaatttg cttctatcgc agcaaaagaa ggaatcatac
    13261 tagatcaaga agaagtgtac caacatattg cttctactta tgatcctgac aacatcgggt
    13321 tgcattaccc atctatgtac caagacctga tcaaaaacca tcgtttgact gaaatcgact
    13381 acatcaatgg tgccatttgg cgaaaaggac aaaaatatga tattgctaca ccatattgtg
    13441 cctttttgac ccaattagtc catgcaaaag aaggaatcct tggcgcagaa taaaaggctc
    13501 tctgatgaga atcagctgaa tcagcagctg gttctctttt tttaagccca ataaaagatc
    13561 aaaaagaaaa cacaactctc atgatgccat aagaattgtg cttgtttgtt tcagtacata
    13621 atctcttcaa aatgtaggtt ttttatttgt tttcaacaga ggagaggtca tattcgtatt
    13681 gctcataaat agtggaatcg ttagtgataa atcgttttgc ccaattttct gcatcataaa
    13741 attccaatag ctctgatagg attggcttaa atggtgtatg cagaaattcc ggtaattcat
    13801 ctaaatcacg ttctcgaagc aaaaaaatat tttcaggacg ataaaaatga taatgagcaa
    13861 tcgcttgatt atccgtcact agtttctttt gaaaatacat ggattcaaat gtccgcaaag
    13921 tcactccttg ctgaccctcc cgcaacagtt ccagaatccc gcgcgattcc cctgtttttt
    13981 tcaggtactc ttcataagta agatatccag gttgttcatt ccctcgatgt ggaagaatga
    14041 aatagttggt cgtaacatct ctttttataa attcctgctt atagctttct gctcgttctt
    14101 ttctcccatt attcgttgca ccaaaaaata gatccgtctt tggttcgcta gaagaaaaat
    14161 tcatatgttt cgaataaaat gaactattat gtttcagccc aagttctttt gcttccaaaa
    14221 aatcaaaatg ataaatctca tcgattgccg gttcatcttg tgcctgcttc agcatcgcct
    14281 cgtgttctgc tttgaagtga ttccaaaaaa ataacacaat ttttgcattg gtaaatgggc
    14341 gcacgcgtcg agccatctcc atatcaaatc cataatctaa aaaaacgatc agctgataat
    14401 ttgccaactg ttgtctagct gatttaatga attccgactg tagtaaatct tttccggacc
    14461 aaacatctaa agagcggatg gaaggcaaaa aataaagtga tttatttttt gcaactagca
    14521 gtacgtcatt tttgttcatc ttttccgcct cactttctct gtcacttttt cctatgatag
    14581 cctgtttaaa tagtaaaaag ccagtaaaac gtttcctata tttgtatgaa aatttaacat
    14641 ttgtatgaaa atatgatgaa ataaatcatt cttctattaa aattcgccta aaagttttct
    14701 gaatagttgt aattgtctga aaatttgtta tcattagtca ttgttaaata ttgctattta
    14761 ttcttaatat aaattttgat tgtgaattta aaagaaagag gtcatttatg gaagataaaa
    14821 aattgaaaaa acaacttagc tccagacata tcaccatgtt agctttaggt ggagcaatcg
    14881 gtgcaggtct atttaaagga agtggcgaag cgattgggat cgccggccct tctgtattga
    14941 ttgctttttt gattggtggg ctgattttat atatcgtgat gaaaggtctg ggaaaaatcg
    15001 ttttaagcgg tggcgacacg catcacggat tatctggttt gatacgccct tatctaggtg
    15061 ctcattctgc tgattttact gattgggtct actggtctat gtggattatc aatatcatcg
    15121 ctgaagctgt tgcagctgct tcctttttgc agttgtggtt cccgcacatt ccagcctggg
    15181 tatttgtttt tatgttggct gttttaacta cgatcatcaa tctttattcg gtacggttat
    15241 tcgcagaaac agaatattgg ctggcttttg ctaaaatcag tgtcattatc ttactgatca
    15301 tttttgctac ttatcttgtc ggtcagcaaa tgttgggcac aggcgttttc cctacccttc
    15361 agcaattgac tgaccatggt gggtttactc ctcatggtat gaagggaatc atcagttctt
    15421 tgcttgtagt tatctattct tacggcggtt ctgaactgat tgctatcacg gtaagcgaag
    15481 cagatgatcc taaacgtgcg attcctaaag caatcaaagg tgtcatggga agaatcattt
    15541 ccttttacat cattcctttg tttttgcttt tgattctttt tccttggaat actttagcag
    15601 gcacgaatgt cagtcctttt gtgatggtat ttgaaaaaat gaatatccca tttgcagcgg
    15661 atatcgtcaa ttttgtgatt gtccttgctt tgttctcatc tatcaattcg ggtgtgtatg
    15721 catcttcaag aattttgttc ttccgtttga aggatcgaaa aggttcctct agaaaactag
    15781 ctgttttaaa caagcatcaa gtaccgcaac gtgctgtttt attttgtacc agtgttcttt
    15841 atcttggtgt agctctttct tattttgtcg gtgacaaatt atttggctat ctggcaggtt
    15901 ccctttcgta tacagtatta ttgatctgga tcattatcag tgcggcatcc tttgtactgg
    15961 cactaagaaa aggaacatta tgggataaag gaatcagctt ttttgcatta gctgtcttag
    16021 gattgatttt ccttggtatc ctgttcacga actccattgg ggtaactgta ttgactggcc
    16081 tgctttacct gttcatttat ttcagttatc aaaaaaagaa tgatgcgttc gtattgacga
    16141 atgaatgaaa tcgatagaaa attttatttt atttatgtgg aatagatgga gctattgaag
    16201 aaaggagttg tgacaaaagt cttgttgcaa ctcctttttc cgaataaaca gtctcatgac
    16261 ctcttggtaa acggtgttca aaaaccctct gtgcaaaata attgattgtt ctccatgctc
    16321 atgacatgat agaagaaagg aatgaaaaaa aggagtttgt tttaaatgaa aaagtattgg
    16381 aagtttttgt tgatgatcgg tgtttcaacg gtagttatgt ttggtatgat gtacttgaat
    16441 gtctatgcgc tcgatcatat cttcttcagt cgtacacgag tattcatggc gttgatgatg
    16501 ggtgcagtca tggcagtcat catgctgttg ttcatgtgga aaatgtatga aaacaaaaag
    16561 ataaatgtta tgatcttagc cgctagtctt cttgtttttg ccggttcctt gtttatggta
    16621 cgaagccaaa ctactgtcaa cgacacagct tggatgaagg ccatgattcc tcatcattct
    16681 atcgctatcc tggcaagtaa acgagctgat ttaaaagatc ctgaagtaaa aaaattagcg
    16741 gaagaaatca ttcaggcaca agaagaagag atcgacaaaa tgaaaaaaat gatcaaagaa
    16801 atggaatgac cattatccgt ccaaataaaa aaagaggatg ttccagaagt tatcagcttc
    16861 aagaaataag aagaagctac ttctggaacg ccgtgaataa ggggttgtga aaaagctgcc
    16921 ctgcatcaag taataagaac agccaaacaa aaatagcttc tcctattttt cgtttggtta
    16981 ggcttattac cgcagatgca agcttttgaa caccgtttat taagaggttg taaaatcagc
    17041 gttctgacct gagtattaga gagacaaatg gaaaaatagt tcctcatatt ttgccatttt
    17101 ttgagctaat accgagggtc agctgattga acaccgttta tcgagaggtt gtgaaaaagt
    17161 cgcttagttt cgcgtattaa gaaaaatttt gtgaaatggc tttccacatt tttgtataaa
    17221 tttggcttaa taccgagaaa ctggcttttg aataccgttt attcgtaaaa gggattgtga
    17281 caaaagtctt gtcacaatcc ctttttatgt ttttattgtt ctaattgcgc tgtcttgctt
    17341 actgcttctt gcggtttcac aaattttttt gagattcccc aacctgtgaa ggcactgacc
    17401 atcgaaaaca caggagaaag caaactgaaa aatacgaatg gcaagtaagt gagtgtagga
    17461 ataccaaatg tacttgctac gaaacttccg gcaattcccc aaggcactaa gtagttgatt
    17521 actgttccac catcttctag cgttctacct aatacagttg gatcaaatcc tgctttttca
    17581 taagtttctt taaatgcatt accaggcaga ataacagata ggaattgttc accaataaaa
    17641 atatttactc cgatacttgt catcaatgtt gtaaaaatca aatttggtgt gttcgtcatc
    17701 tttcttgata ctcgttccat cacggttgag atcaagccaa attcgatcaa caatccacct
    17761 aatgacaaag ttagaataat caatgacaca gtaggcatca tactttcgat tcctccgcgg
    17821 ctaagcagtg aatcgatttg ttggtttcca gtatttgaaa caaaccctga ttcgatgacc
    17881 ccagccaact tcgttaagga agtttgtggg ttttgaataa aaatcattcc tactgctaca
    17941 gcgatattca acaaaatcgt taaaattgcc ggcacctttt tccatgcaca cagtaacatt
    18001 aaaaataaag gaatcagtga ccagattgaa atagtaaagt ttgcttctag aacatcagtc
    18061 acttcacgaa tagatgaaag acttgcggct ttacttgtgt gtcctaaaat catgaataaa
    18121 aagaaagaaa caacaaatgc cgggacagtt gaccacatca tatgcttgat gtgtttgaac
    18181 aaatcggcat caacaatagc tgctgctaga tttgttgatt cggataatgg agacatctta
    18241 tctccaaaaa cggcacctga aacaatagct ccaacaacca gcgctggatt taagcctagt
    18301 gttgtcccaa taccgaaaaa tgcaatccca atcgttgaca taacagtaaa ggcactccct
    18361 acagcgctac ctacgattgc acacacaaca aatacagaag ggacaaacca ctggacactg
    18421 atcaatttga acccaatgac cattagagtt ggaatgatcc ctgcttgtat ccatacagca
    18481 atcaatgaac cgactaaaat aaaaataaag attgggatga ttcctggttt gatgccattg
    18541 atgattcctt cgtgcagcca atctgtcggg tatcttttta tcactgcata caacatgacc
    18601 attccgatcg ctgctaagat cgtgatatta ggtgagacct ttaatccgac aacgccaatc
    18661 gcaatgccta aaacaataac cattaataca agtgttgctt ctaaaaaact tggtgttcct
    18721 ttttttccca tgataatctc tcctttttta gttgttctta tgttctaaaa aagttaagat
    18781 atcggattac tccgccgcaa ttcatccgcg attcctcctt tataaaaata aaaagacctg
    18841 ttttaaacta aggacgaatg actccgcggt accaccttaa ttaaaaacag atctgttttt
    18901 cactttcaaa cttttattca aatgtatgga ctttccatgt aattcgtcaa ttaggcttga
    18961 tcggttcaca gcgaccaccg actttctgaa caaaacacct aaccgctact gcgtgaaaag
    19021 ttgacttgta cgaagtttac cttccataag atcatttgtc aatgaaaaag aaaatatttt
    19081 ttcatttttc tgaaaattat ttttataact ttcacacaca agtttatttt ttactagaaa
    19141 gtagcgccct cttgtttttt tcttgctact atttcttgat gttcttgctt gattctttct
    19201 aatagctttg gctctttcaa cagatcaaga gctgtattgg cgagtagttc tgctgcaatc
    19261 gcaatagaag caaggccttt gtcactttta gcagcagctt tgaattcttc tgtgtgtccg
    19321 gcaatatatg tctcagagat tgctaccgta ggctggattg ttggtatgac ttggctgaca
    19381 tttcctacat cagaagaacc gagacttgtt ctttgttccg tttctatttc ttcatctgga
    19441 acacctgctt cttttgcatg ctggaagaat aagtcatcaa aagaaggagt aacgatgaca
    19501 tcatcaactg cattttggaa caatccaaac tcaaaagtcg tgcccgtact tatcgcagca
    19561 cctcgtacga tattttccac tttttcatat actttatcta atgtttgccg atttcctgca
    19621 cgtaaataga aacgtccagc agcgtaatct ggaatgacgt tggcagctac tcctccgtct
    19681 gtaatgatcc catggatatt gacatctttt ggcaattgca agcgtagagc gttgattcca
    19741 ttaaacactt ggatcaatgc ttccaaagca tttactcctt tttcaggtgc tgctgctgca
    19801 tgggaagcga caccgtaaaa tttgatgtcg actggatcat tcgctaaaga ttctgtcgtt
    19861 ttcccataac gaaaagctgg atggacgcat aacgctacat cgacatcctc aaaaaaacct
    19921 tctcgaacaa agctgccttt agctgatccg ttctcgcccc cttcttcccc tggggtgcca
    19981 tatacacgga tttctccacc agtttcatca atgaattgct tcactatgct ggcggctaat
    20041 acggagtaag taccaaacaa attatgcccg catgcatgac caatacctgg taaagcatcg
    20101 aattctgcca aaaaggcaat tgtgggaccg ggtttattgg aaatataacg tgcatcaaag
    20161 gctgttcggt gtccagctac ttctttttct acccaaaacc tttctttttc taattgttta
    20221 atcaacgctg ctgaggaata aaactcatag ttacttactt caggatgttc gtggatatct
    20281 agagcaagtg cttgatattc tggtaaacga tattggatta gatccttcaa ttggtttgtt
    20341 gagattgttg ttgtcataaa aaattcctcc tatgctagtc atttttctta ttttctcccg
    20401 aatgtttccc atgcaggcac gcttgatcct tttgatgttt tctcgattac tttgactgtg
    20461 tcttctgttt gataagcgtc tactattttt tgatacgttt tattgttttc atcttctttt
    20521 cttgcaacga tgatattcac ataaggacgt gcatggtcat ccactggttc taagaagatg
    20581 gcatcagaag caggattaaa tccagcatct acagctacac cgctattgat cactgaaatg
    20641 tctacatctg acaaagcacg tgctgtttgg gaagcatcta attcagaaat gtctagattt
    20701 tttctattct cggtaatatc agaaacagtg ggcgcttgtc cttttgcctt atccactttg
    20761 atcaatccag cagtttgcaa taacagaaga gctctgccgc catttgtaac atcattaggg
    20821 attgctactt ttgctccgtc tttgatatca gctacatcct tcaaagtgtt agaataaatt
    20881 cccagcggtg cattgacagt atttccgatc gatactaaat cagttccgtg ttctttattg
    20941 taattgtcta aaaagaattg atgttggaaa gaattcaaat caatcgatcc atctgctaaa
    21001 gccacatttg gttgattgta atctgtaaat ttcacgtatt ccaaattaat gttctccttt
    21061 ttcaaacggt ctttcacgtt gtcccataca tctgtatctt ctccaataat ccctagtttt
    21121 acgatagttt cttttttgtc agttgtatct gcttttcctt ggctacaccc tgccaaccaa
    21181 ataattgcta caaatgtaat gactacaaaa cgaataattt ttttcatttt ttctcctcca
    21241 ctttcaatgt gtaatttttt taatcagttg attactgata aattgactta aaaaaactaa
    21301 gattagaatc aataatgtag ccacaatcgt tacatctgtt tggaatcgat tataccctct
    21361 cgatatagca agattcccta atccgcctgc acctactgct ccagccatgg tagtcagtcc
    21421 gatcacgttg atgatagtca acgccgagac ccgaatgata ccaggcaaac cttctaacaa
    21481 atacacacgt aaaatgatac cgattggact ggttcccatc gcttctgccg cttcgatcac
    21541 cccaggatca acttctagca gtgcgttctc gatttgacga gcaaaaaacg gaacaactcc
    21601 aaaaatcaat ggcactaaag ctgctttcgc accaatcgtc gttccaacta agaaacgagt
    21661 agtcaatgct aaaagagaaa gcaagataat gaaagggatg gaacgaatga tattgatcag
    21721 cttatcaagt atttcaaata gcagacggtt agctagtaat ccattagggc gtgtcactac
    21781 taacgtcaca cctaagatga ttccaatcat cccgccaatg aatgctgtcc aaaaagacat
    21841 atatagtgtt tgcttcgtcg cttcgacaaa ttcatccgga atcaaataaa cattgggcaa
    21901 atatgtttta aagaaatcag tcattttatc ttcctccttc gatcactttg atacgactat
    21961 ctaaatcgtt tttttgtaaa atatttattt ttacaccaga tttgatcaaa taatccaatg
    22021 cttttttccg ctgtttggtt tctccagcta aagtaacgat caaactccct aacggcgttt
    22081 cttgtaataa ctctacattg ccatataaaa tattcgctgt tacttgatat ttgctgtaca
    22141 gatgagcgat tagaggttcg ttcgtctgat ttccaatata acttaattcc accagccatt
    22201 tattggcgat ctgatcggca aaagcagaat gggcaatgat cgtttctaat gcttggtcaa
    22261 gatgagtagc tgttcgaata aaatctttgg tcaattcttc ttcaggattg ctgaagatct
    22321 ggatgctgct acctttttca acgattcttc catcttccat aaccgctacc ttgttgcaga
    22381 tttctttgac aacttgcatc tcatgagtga tcaggacgat tgttaaacct aactggcgat
    22441 tcaatttttt taacaatgca aggatctgaa gggtcgtttt cggatcaagt gcactggttg
    22501 cttcatcaca taataatacc ttaggttcat tcgccaaagc tcgtgcgatt gctactcgtt
    22561 gtttttgtcc ccccgacagt tgtttaggat aagcagatgc tttttcttcc aaaccgacaa
    22621 gttccagtag ttcattcact ttttgacgac gttcctgttt cgattttcca gaatacttta
    22681 aagaaaaatc aacgttgtca aaaatagttc ggctttccat tagattgaaa tgctgaaaga
    22741 tcataccgat ggcctttctt tcttttcgca attcttcggg agagagctga ctcagattcg
    22801 tctgattgac aatgacttct cctgctgttg gtttctgcag caaattcatc acacgaacta
    22861 acgtactttt ccctgcacct gagtaaccga cgattccata aacatcgcct ttttcaatcg
    22921 ttaaatctac agaatctact gcctgtatct ctttttcttt ttgtttgaaa catacagaaa
    22981 catctcgcaa ttcaatcatt aaaattcctc cttatctaaa aaaagcgtcc tcaagaaaat
    23041 gaaaatgtca ttttcttgag gacgcttttt tgcgtgttac cacctcatgt tcaagctgtg
    23101 ttcacacaca actcctcttc aagtacgatc atactctagc aagatatcgg tcgcagccgt
    23161 catcgcctta catctatgct cgggatgaat tgctcgatgg ccattttcca atagtcgttc
    23221 cttgcttctt ctcagctacc gaagctctct gtcaaaggtt cttctatcgt acttaccatt
    23281 tcaatgcaaa taatattcaa aaaaaaagtc cttttactaa cataaactat gtcagtaaaa
    23341 ggacgacgta tcgcggtacc accttttttc ggaagtttcc ttccctcaag cccaacatct
    23401 caaaaagaga gattggcgtt cgttttaacg gtcgaacagt tccgaatttg cctacttatc
    23461 gacaaattat tctccaaggc cattttcagc ttttcttact tgactcttct caccagccag
    23521 agcctctctg caaagtttcc aaacttactt tccttttcaa cgaaatcgat attttgttgt
    23581 tgggattaat ctacatgagg aaaactgaaa agtcaagtac gaaattcaaa ttattttttc
    23641 tatatttatt tttctgtttt tttatttaac atttcaataa cagttcgaag atcttttcct
    23701 tccgcaagaa gtttcaattt ttccttttct tcgacggtat aatttcggac aaaactggaa
    23761 tcaggtatca aaacaacgcc taatcctgct cgtgaagcag ctgttgctcc tactaaagaa
    23821 tcttccactg caaaggctga actagcttct aatcctgttc gttccaaagc tgtcagataa
    23881 ggaatcggtg aaggcttgtt ctccgacact tcgtctccga atgtaaacgt gtccaagtaa
    23941 tcagctaatt taaagtgatc caagatatct gttgctcttt tcgtaacagt ggtcgtagca
    24001 atgcctagtt tcatatgacg ttcttttaag tctgccaata tttcttttgc atatggtttt
    24061 aagtggatgg ctccgcttgt cagctgttga taaaaatatt cttcacgctt cgctcggact
    24121 tcctgtactt tttcagggct acctgctgct ttcactaaaa tatcaaaact ttgtttccag
    24181 ttttttccgc cccaagcgtc aacgatttct tttgggatat ggatatcaaa ttgctgaaat
    24241 ccccaaagcc agccatcacg atacgttttt tctgtgtcaa ctaacaaacc atctaaatca
    24301 aatattgcac ctttcatcta tacattaatc ctttccaaaa caatacacat agtttagcac
    24361 gactggtcca aaaagaacag aagcaaagaa ccagccttct cgtttataag agttcttgga
    24421 atcgacggat atagattttc ttcacagctg tcacaagcgc cagataagcg atgactgtag
    24481 caaataacca taaccagtaa ttgccaggaa gtggcatcaa gcctaattca gcaccaaaac
    24541 ttgtaaaagg aaggacagaa ccgacaataa ttcctaatgt agtgataaca aacatggcaa
    24601 atgttgcgtt gctttggata aatggtactt tcggtgtacg cagtgcatgg agcactaagg
    24661 tttgtgtcca tagtgattcg acaaaccatc ctgcatggaa taatgcgatg aaaatcactc
    24721 gctgtgaact atccaatgta tggaaacttc cgcccactac agcaggacaa ataacaaaat
    24781 acatcaacaa gtaagtagta atatcaaata tcgaacttgt tggaccaaag taaaccatga
    24841 attccccaat actagatgca ttccattttt ttggctcgtc caaatattct ttatccatcc
    24901 gatcccatgg caaggaaatg caggaaacat catagatcag attcaaaaac aaaagttgta
    24961 aaggcagcat tggtaaaaat ggcaggaacg tacttgcaat cagcacggaa aacatattgc
    25021 caaaattaga acttgctgta gccttgatat atttcatgat attcccaaaa gtttctcgtc
    25081 cagaaagaag gcctctttcc aaaatcgtca aatctttttc taaaaggatc acgtctgcag
    25141 attctttcgc aatatccaca gctgtatcta cagaaatacc gatatcagcc actttcattg
    25201 caggagcgtc attgatacca tctcctaaaa atccgacagt atgaccattt ttacgtaaag
    25261 ctgtcgtcaa acgtgctttt tgctgaggat tcaattttac gaatacttca tggtcttcag
    25321 ctacttgtgc taattcatga tcactcattt ccgagatttt ctcaccggta accaattcat
    25381 tgatctctag acctacttga cggcacactg aacgtgtaac taattcgttg tccccagtca
    25441 gaacttttac agctacacca tgttctttca gagctttcaa cgcttgtttc gttgtttctt
    25501 ttggtggatc aaggaaagca agataaccaa tcaaaaccat gtctgactca tctttgactg
    25561 aaaattctcc aacgacgctt ggattggtct tttgtgcgac accaatcact cgtaagccat
    25621 cttcattaag tgactgtact tttgcaagta cggcttcttt tcgttctttt gttaatggtg
    25681 taacagtacc gccaagatca ataaatttag aaatgttcag catttcttca atcgcgcctt
    25741 ttgtgatcat ttgtgtttta ccagctggat cagcaaccac aacactcata cgacgacgtt
    25801 caaaatcaaa aggaatttca tccacttttt gataattgat cttatctgta tctaaagttt
    25861 ctgttgcttc atcaatgata gccacatcta acaaattttt cagccctgtt tgatagtagc
    25921 tatttagata cgcatggcgc aaaacacgat catcttcatt tccttcacaa tccatatgat
    25981 attcaagaat gattttatct tgtgtcaatg tccccgtttt atccgtacat aacacatcga
    26041 ttgcaccaaa gttctggatg gcattcaaat ttttgatcac tgttcctttt ttggccatca
    26101 cacttgctcc tttgaccaaa ttcgtcgtaa cgatcattgg aagcatttca ggtgttagac
    26161 caactgctac agatagtcca aaaagaaaag cttctagcca atcccctttt gtaagcccat
    26221 tgatgacgat aactgctgga gccatcaatg acataaattt gatcagtagc atggatgttt
    26281 tatggatgcc taattcaaag cttgattgaa ttggtttttc agatagcgtt tctgctacat
    26341 gaccaaatag tgtttcattt ccaacagaaa caatgattcc ttccgcactt ccactgatga
    26401 cattacttcc cataaagaca agattttcgt aacttgtttc gctttgctga tcgtcttcac
    26461 atactttcgc atgcttttct actggatagc tttcccctgt caatgctgct tgggaaatga
    26521 ataaatcctt tgcggacaat aaacgcatat ccgctggtat catatcccct gctgaaagcc
    26581 gaacaagatc tccacagacg atttcttcca taggaatctc tgtgtacttt ccattacgtt
    26641 ttacagctgc cgttactttc acaagtgatt tcagtttttc agccgcttga ttggaacgta
    26701 cggactgaat caacgtcatt gtcccgctga cgataaccat agtgaagata atggctacac
    26761 cgaaaagatc tttatctcca ggagctgcta atacatattc cgtgatgaat gaaataattc
    26821 ctaatgcgat caacactaat gtaaatggcg tgatgtatgc tttcaatatt tctacaatga
    26881 atggtgtttt tttgccatat gaaatcgcat tttcaccgta attttctctt gaacgttcga
    26941 tttgttcttc tgtcaatcca tttgctgatg catggaatag ttcaaataat tcttgtttcg
    27001 tttttccagc aaaagctgca tagatttttt gtgtatcttt ctttttttcc atctcaatct
    27061 ctcctttgtg tccaaaagac agctgttctc gctgcttttg gtctttagct attcatcgga
    27121 tcgatagaga aaaaactgaa aggggctgag aacagataga taatagccct ggcctttcct
    27181 ctagatcctt gtttttcgaa tgactgtcat ccatttgttc tcatctcctt tactaagtaa
    27241 ttttaattca tgacttccaa tcatttttcg aatagttatt tttccaatta gaccacctcc
    27301 tttttatttt tcaccgatgg ggatttgcgg caacaaaaaa acctctcgcc aaatactcag
    27361 cgaaaggttt gaaaaagcat attaaaaaaa gcttctcaga tctcatcgtt gagttttgac
    27421 actgtacaac gtaaaaagca gatacctgct tttagctacc ttatgaaaag ccttatctcg
    27481 acaattcctg ttctacccat tggcgtctct cgacgttttt gggcagtagc ctatgtttgt
    27541 ataggagcct cacctaacaa atcttattcg gttttccttc gttaatataa caactaaaaa
    27601 agaggatgtc aaatcaattt tccatgtttt tcatactttt tttaaaatac acacgttata
    27661 ttcctgtctc acgcggtttt atttgtctca ttttccacct cccgctataa tagaaatata
    27721 gtaggaaacg aggtgctgtt atggaaagaa cacttgttat tatcaagcca gacggcgtga
    27781 gacgccattt agtcggaagc atcatccaac gttttgaagc aaagggactg gccattgctg
    27841 aaatgaagtt tgaaactatg actcctgaat tagcaaaaga acattatcag cacttgactg
    27901 agcgcagttt ttttgatgaa ctgatcgatt acatgacttc cggtcctgtc gtttatcttg
    27961 tgctggtcgg cgaggaagtg atcgatatcg tccgtaagat ggttggagca acaaaagctg
    28021 ccgatgctgt tccaggtacg atccgaggag actacgcgct gccggggact gaaaacatca
    28081 ttcacgcttc tgattcccgt gatgctgcgg taaaagagat cgcccgcttt ttcccgccat
    28141 tcgatataaa caaaccgtct gaaaaagcgg cgaagtgata caaattctaa actgctggtc
    28201 tagttgctgg gttcagcaat ggccagcagt tttttctcta aggacctccg ttgattcttt
    28261 cctaacatat attcgagcac tgtgaagaat tcttggtagt ctttcgtctt ttcttcccct
    28321 aaagcagcag aaatcaatct ttgataataa tctgccatca atgtttcata gatcactgta
    28381 cttctttctt tatgtgctaa gctccgtttc atatcttcat tcacactacg tgcctttttc
    28441 aaccgaccat tttctatata aaacgaagtc aggttcatta aaaaagtaaa gagatactgc
    28501 tcatgattgt tcaactgtgc taatcgtcgc attctttttt tgattttcaa ataattgttc
    28561 tcaatgaacc gatcactgaa tatccaagga aaatcagcta gaatagatag ttcaaattga
    28621 ccatagtctg gtatctgcat caagtattct tcaatgattc ggcattcata acgtaaaaat
    28681 tcaaattctg tctcagatac ttctgttttt tcgaccatgt gaagattcaa aaccaaataa
    28741 tacagatctt ctgttttctt aaaatgccta cggttattgc tgatctcttt ataaggatca
    28801 aagctgtctt cttgtacccg atctagcatt cgtctttttt tctttaactt tattggttca
    28861 tccagctggt ttcgcatcca ttcatattct gcaagtgtca tgttcattcg ctctaaaaat
    28921 gatctcaatg ttacaaatga catttttttc actgaatttg tctcaatccc gactagagaa
    28981 gtcttaaaac agattccttc cgctaattgg gactgtgtca acttcttgtt gactcgaagt
    29041 ttcttgatca attcaccatc atacataata gttctccttt ttaatttaaa tttttccaca
    29101 ttcaataaat agatacgtta aaaaggaaaa atacacgaaa aaaataaaaa aatgtttttt
    29161 tcataaaaaa taccaacctt caagcattag aatgttgtct aactactgaa ggttggtttg
    29221 attgtttatt gattattttt ttgctgttca acgtctgttc ttgttactgg atataagttg
    29281 gttgttgttt tattaccatt ttttgaaaag acacttgtcg aatgttcgcc tttttgcttt
    29341 tcgattgctt cattacgttc cgcttcattt ttatagtcat attttttcgg atcaatcgtc
    29401 tcaaatccgt ttggcacata aaaccgcaag agattttcct gattcagctt atctgaaaga
    29461 cttaacgcag tcttgacttt ttcctgatct ttggagattt cctcatcgat tcctgctgct
    29521 ttcgtatcga tgatttctcc tgtctgatta ttgtaagctt ttcctcctag gacggtgtat
    29581 ttgggtgtca caaagttccc atttcgaaaa gcaactactt gatcatgctg agaagataat
    29641 aagtctgtac caaaatgaag ataatcttta tcatcgattc ccaatagatg tagtaatgtc
    29701 ggcaaaacat cgatttctcc accgtattca tgattgactg tccctttagt ataaccaggc
    29761 atatggatca tcaatggcac tcgttgcatc tgtgcgttgt caaattcatc ccatgtatct
    29821 ggatctttac caagagcgct tgctaaatct ttattatctg tattcgagat cccaaaatga
    29881 tccccgtaaa tcacaaagat tgaattttgg taaataccag attttttcaa atacgtaaaa
    29941 aattgttcca atgattgatc cagataatgt gccgttcgaa cataattatc tactgtgcta
    30001 ttgccagtat tcaaagatgg aaagtcgaaa ttcttgtcat ccgtataata aggcgtgtgg
    30061 ttggttacgg aaagaaattt cgtgtaaaac ggctgttgta gatgctcaag atattgggca
    30121 gattctctga ataaatcttt atctaatatc ccataaccta acgtttcgtc ggattcatca
    30181 aaataagatc gatcaaagaa attgtcataa cctaagtttt tatatacatg atctctattc
    30241 caaaaactag ctacattccc atggaaaaca gcgcttgtat agccttgctt ttgtccaagg
    30301 atcgctggtg cggcttgaaa gacattgtct gacccaagtt ctgtgaataa tgagccttga
    30361 ggtaaaccaa atgtgccagt ttccaacata ttttcagcat cacttgtctt tccctgtcct
    30421 acttcatgaa agaaattgtc aaagctgatc gttgcttgat tctggaaaat actatttaaa
    30481 aacggtgtga cttcttgtcc gtctaccttc atattaatca aaaactgctg gaaactttct
    30541 aaatggagaa cgatcacatt tttcccttta gcgatcccaa atgtttcggg atttggctct
    30601 gcataatgtt tctttgtata atctaaaact tcatcaatcc cattactgct tgcatgcgcg
    30661 cgaacactgc tagtcattcc tgttttgatt ccgtcataaa tagtatacgc atcgattcct
    30721 agatatttga caatatatga acggtcaaat gttcgctgca gcaattgtgg acggttggct
    30781 tcgctcagcg ctagatttcc tgaaaaaata agccctgaaa gacaggtaac agcgatagcc
    30841 atcggtttgc cgacagggtt gcttttgata ggtattttct tgaccttcaa ccaaaccagt
    30901 agcccaatga acacagcgat atccagccag tagatgacat catggggctt catcaaagaa
    30961 aaggagcttc ctgacaatcc ttgcgacact ttcgaaaagc ctaatacaga tttgactgta
    31021 atgaaatctg tgaattcccg ataaaaaata atattcaagt acaacaaaag cgtatttaaa
    31081 atatcaatga tcaaaagaat agctaacgct ggctttggct ttttaatata caaagaaagg
    31141 ccaaaaaaca ataaggtagt cgcaattggg ttgatccaca agataaagta ctggatcgta
    31201 ccttccacac ctaatgaaaa atctgcatag taggcaatga tcgtcttcag ccagaaaaag
    31261 aaaacagctg tcaaaacgat tgctaaacga ttttggcaca attttttaag ttgtgtcacg
    31321 tatatcactt ctctctattc acattttttc ttattttgct cctgaaaaat gaaaatttcc
    31381 taaaaaagtt gttatcttaa gattttttta ttctttctaa agattattcc gtccactttt
    31441 cacagcataa aaccgctgcg atgtaagcga ttaaactaat gaagagataa aagtcttgtg
    31501 gtgaccgctt cactccacgg tattcaaaag ccagtttctc ggtattcgta caaaaaaaga
    31561 ggctgtgaca aaattcttgt cgcagctcct tatttcttga tgcaggacag ctttttcaca
    31621 acttcttcct taaacggtgt tctcctgcca actcctcggc cacaagtcac aatatttata
    31681 aaacatgaga agctgttttt tgaaattgtt ccttgtggct cagagttaaa cggcaggctc
    31741 aagacctctt gatacacggt gttcaatcag ctgaccctcg gtattagatt atgtcaggac
    31801 gctaatttta caacctcttg gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    31861 caaccaaacg aaaaatataa ggagctattt tttgaaattg ctccttgtgg ctcagagtta
    31921 aacggcaggc tcaagacctc ttgatacacg gtgttcaatc agctgacctt cggtattagc
    31981 tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg tttctctaat actcaggtca
    32041 aaacgctgat ttcacaacct ctgtttttca ttgtccatag gattgatatt gtccttgtga
    32101 tgactcattc gtaataattg aggcttgatt ttcttcactt cccgttatcg tttgtccact
    32161 gccagaatct gaaggacttg ttcctgtcga ctcgtttttt tgagaagaga cagttgtttt
    32221 cttgacttgt tcatttgtct ttttccattc cgctaaacga tcggcgacca cttttttgtc
    32281 tactttttca gaataagcgg tgatcacttt tccatttttc aaacgggcta ctttgtcgcc
    32341 atcatgagtc aaaagcaaaa cggtcaaatg cttgtcttta aattgatatt gtctgagaga
    32401 aatcgcacta agtacgtcat cgatattaat aaattgattt tcttcttttc cttttggcaa
    32461 ataaatgtcc aattcgcctt tttcgtaatt ggcagttatt ttatactccg cccaccaata
    32521 agttgagaat tcttttttga aattatctac tgattctttg cttgctacga ttttttctgt
    32581 ttctttctga gcaagatctg ctgtcatttt tcctgaaaat gctgcggata aaataagcag
    32641 taagaaaaaa ccgaataacc aaaagagaag aaacggtctt tttttcctca tgctttcctc
    32701 cactcctatt taattttgac tattctatta tatacaaaaa atgaaataga aagacaaaaa
    32761 agttgaaaaa gatcgtaaat aattgaactt ctttttcaca aaatggtaag aatggagaat
    32821 ttcacgcatt tgctctcaac tattctttct taagcctcaa atccgttttt atctgatacg
    32881 tctttgaacc aatgacccga ttttttgatt gttctttttc catcttgtgc taagtctact
    32941 gaaataaagc catatcgatt cttatatgca ttggtccacg accagttatc catacatgtc
    33001 cacatatgat agcctaaaca attggcacct tcttgaatgg cttgatggac atatttcaga
    33061 tgagattgga caaattcgat ccgataatca tcgtggatca tgccatcttc tttgataaat
    33121 cgtgcttcgt tttctacacc cataccgttt tcagaaataa agcagtcgat atttccataa
    33181 tgatctcgta cattgatcaa aatatcatag attccttgtt catagatctc ccatccgcga
    33241 taaggattca ttttacgacc aggcatttca taattgtcga aataatcctc aggcattgga
    33301 ccgtttttag tatcgattgg tgtttctttt gcttttaccc ggcgtggctg gtaataattg
    33361 atacctaaaa gatctactgt attttctttg ataaccgtca aatcctctgc atggatcaca
    33421 ggcatgagat caagttcttt tactatctca atcaattcac ttggaaactc gccttttaca
    33481 gctggatcaa ggaatgaacg attgaagaac gcatctgcta tttgcgctgc tttaacatct
    33541 tcagggtttg attcgtctct tggatagctt ggtgtcagat tcaagatgat accaataaca
    33601 ccaccttgct ccatttcatg gtaagatttg atggctaacg ctgaagccaa cgcttcatga
    33661 tagccaactt ggatcgcttt tttcatatct gactcttctg gataatgcca tcgatataaa
    33721 taacctgctt cgactggaac gattggttca ttatgagtaa accatttttt tactttcgaa
    33781 ccaaacaatt caaaacaact tttggcatat ttttcatagg cttcaaccgt ttctcggttc
    33841 aaccatccgc ctttttcttg cagatacatc ggcatatcga aatgatacag attcacaaag
    33901 ggttcgatac cgttttcaat caactcatca aaataacgat gatagaaacg aacggcttct
    33961 tcgttgattg ttccagattc atcaggtatc aaacggctcc attggataga tgttcgaaat
    34021 gaattatgcc cagtctgttt catcaactgt acatcctctt gatacttcgt ataaacttgc
    34081 gaggtttttt caggtcctac ttgatcaaag aacttttgcg gttctttttt gtaccaataa
    34141 tcaaaaatgt tctctccttt tccatcgcct gaaatccttc cttctgtttg tgggccactt
    34201 gctgcagaac cccaccaaaa atttgctgga aattgatatt ttttcatcaa agcacctctt
    34261 tttctttttt agttcgtttt agttgattcc atgtatgata ttttccatca caatcacttt
    34321 ttcattttct tcctcttctt ttttgattcg ttttaacagc tgttctgctg ctcgttcccc
    34381 gattttatct gttggtggcg tgatcgtgat tttcggaagg atactcagat cgtttttatc
    34441 taaataatca tagtgactga acgcaatatc cttcccgatt ctgacacctt cttgattgaa
    34501 acattcgatg gcaccaatcg acatattgta attgctgaca aaaatagcag ttgccccttt
    34561 ttctaataat gcttttgccc cgtaatagcc gctttttcta gaatagtctc ctgtaaagac
    34621 ctctaattgg atcgatggat cattttggac cgcttctttg gctcctgcta aacgttctct
    34681 tgctacgtag tctgtttgag tggcagcaat aaatccgatt tttttatgtc cttgttcacc
    34741 atgatgagcg atcacttttg ctacactttc tcgatcattc actaatattg tatcaatacc
    34801 tgctggtcct tctggacaat tcaatgcgat aactggaata ctcatttttg tgagcatttc
    34861 aaatccagac cactcttctt cggataagaa gacaatcagc ccatcgatca catgcttcat
    34921 caggtattca attttttgtt caattttttc tggctgatcg gcaaacccgc ttaataataa
    34981 gctataccca ttttcttcag ctactctttc gatccctgag acaacttccg acccaaaacg
    35041 gctggaaatc ccgtttatca aaagtccaat agagaagctt cgattattct tcaacccttt
    35101 agcaatgatg ttttctttat agtccaacgc tgaaatcgct tctgctattt ttttcatgtt
    35161 cttttccttc agttgctgac cattcaagta acgggaaact gttcccactg aaacaccagc
    35221 catcttagcc acatcacgga tcgatgccat acattctcac tcctgccagt tacatttgat
    35281 ttctctttct tgttgttcga ttatgatgac cgcattcttt gcatagtgtt gcttgtcatc
    35341 aatatccata taactaaagt cttcagtaga gcaacttcct ctcactatta attatactta
    35401 aacaagtatt tgaaccggtt catttttaat atagcatttt tttaaaagat tgcaacactt
    35461 ttttaaaacg ctttctgaaa gtggcggatt ttttgccttt ttaaaatttt attctaaaat
    35521 ttactctatt tgtgggtgtt ttgtgcgttt ttctcttcca tccgtttgaa gttttacatt
    35581 ccaatggtat aatttcttta tcctatcaaa tgggggaagt attttgaaga aattaatcgt
    35641 actatttatt ttgtctgcgg gacttttgac attgactggc tgtgatttgt ttggcggctc
    35701 tgaccaatcc tcggacgaaa caaccgattc ttctgtctta aaagcggaaa aagcagatac
    35761 caccaacagt gtatatacgc taaaagtacg taaagatatg gatttatccg cgggacagtt
    35821 gacatttaca gataaaaaaa tggaatggac acgcacgtat cagacacaag gaactgattc
    35881 ttctgaatca tctgaagaaa aaacagtgct gacagatatc aagatcacta ccaaagatga
    35941 cgaatatatt atttcaggaa aagaaaataa aaaagaaaca acagtcatat ttacaaaaat
    36001 cggtaattac cgtttgaagg atgaggatgg caacatctac agtctatagt gccgtctgtt
    36061 ggatcttctt ttgtttctcc ccttcttttc tgctatagta aagaagcaag atgattgtaa
    36121 aggagacgaa atggatgatc gatagtcctg attattgtac atgtaataac aacaaaaata
    36181 tccatacaga atttaatgac caaggaagct tgagcatatg cgacaactgt cacaaactag
    36241 tcaaagattc atttaaaaca tttgaatctt atcctgttca aaattatgta aaaagtagaa
    36301 gaagataaac aaatccaagc aaaactcact atggctgatt tttatgaact tcaaactcag
    36361 cttcttcctt tgatcaagct tcatcagtaa aacaaacagt ggatcatttc agtttacaaa
    36421 tcaaagagta aagatttatt tatttcaaat atttgaatat aaaataacca aacgatagaa
    36481 aggcttattc tacatcacgt agtaattttt ctttcccgtc gtttggtttt ttatttttag
    36541 cctaattctt gaccttttgc atgctggtta tccgtaaaat attttccagc aggctttcct
    36601 tccaattcag ctaatttttc ttggatctga cggatcacct tttttgcatc ttctggattt
    36661 tctacaaaat cgaatttatc cccatccact tgtattttag gacatacatc aaaatcttca
    36721 taccattgat tatagcgggt gttcaacgtt tcataatagg tatataacgt ctcatcgctt
    36781 tctagctgtt catagtcacg cccacgtttt ttgatacgtt ctagcatcgt gtccaaagaa
    36841 actttgatat ggaccatcaa atcaggccgt ttttttggag caacatcatc taactcgttt
    36901 agcatcgtat ctaataagct atcgtactgc tgtacttcga tatccgttac ccgacctaaa
    36961 tcagcgttta gatggaacaa caggctatct tcatagatgg atcggtctag cacattgtca
    37021 tcgttcacta aagcattttt cattgctaaa aatcgcttgt tcaaaaagaa gatttgcaat
    37081 aaaaaagcgt actgctcagg atttgaataa aagagcggca atacttcgtt atcttcgaca
    37141 ttttcataaa atggtttaga atcaatctct tttgcaagca tatccgtcaa actgctctta
    37201 cctgcaccaa tcgttcctga aatcaaaatc actgacatac aacccttcct cttctataag
    37261 tactcgccta cgtaagtttc aacaggcaat gtttcaaaat catattcacg tacagcttgg
    37321 tctgatttta aatacgtatg gagactttgt gtaatcatca agttcgtagg taccgtctct
    37381 tcttgtacaa tgatcatcgg ttttttcaac atagttgcca cacctaattc gtaagccgtt
    37441 cctggatcga ttgtttgatg ctcaaagtca aggatagcaa caacaaattc tgcatttgtc
    37501 agttcttcca tatctttttc gtatacttcc tgcgcccatc cagcgctgaa tagttcataa
    37561 ttgctttctt ggtgatgacg tggagagtag aaacttgtga ctgttttgtt ttcctccaac
    37621 gctttttcga tacgacttac tcgatcgatt tgctcctctg aaaaaaatgg tcctgctaaa
    37681 tatatttgac tcattatttt gcctccttca ataataactg atttttaata gatactgctc
    37741 gtaattcatt gaccagttcc aatggattgt tggaaaagat tacttgatcc aaccattttt
    37801 tatccataaa atcctctttt gcaaaagttt ctaataattg ttgcaagccg ctgtaataac
    37861 cgccaatatt caatattccg attggcttgt cgggcgtcag accgattgct atccaagaaa
    37921 gaagttgtga gaattcctcc aatgtaccta gacctccagg gaaaacgagg acagcatctc
    37981 ccttttcaaa taataatacc tttctttcat cgatcgtttc tgtaggaata aatgtagtaa
    38041 catcttttcc aggcaattca gtttcaaaaa gcccattcgg atagatacct gtgacttttc
    38101 cgttattttc cagtactgtt ttactaatca ctcccataat cccggagtat gaaccaccgt
    38161 aaaccagttc gatttcttct tgtgccatgt attttcctaa cgtttgcgca ataaatttgt
    38221 aactttcttt attaccaaaa cgggaaccgc aaaaaacagt aagtttcata aaaaaacact
    38281 ccctaactca aaagtctatc ttcattctac acgtgttttt ttaaaataaa agactagatt
    38341 ttgtagtttt ttagtggtat
//
LOCUS       DAZKBH010000015        35527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000015 DAZKBH010000000
VERSION     DAZKBH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.015"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..1085
                     /locus_tag="ACG8OO_000771"
     CDS             15..1085
                     /locus_tag="ACG8OO_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9044698.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1100..1768
                     /locus_tag="ACG8OO_000772"
     CDS             1100..1768
                     /locus_tag="ACG8OO_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044699.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1788..2366
                     /locus_tag="ACG8OO_000773"
     CDS             1788..2366
                     /locus_tag="ACG8OO_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9044700.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2769..3680
                     /locus_tag="ACG8OO_000774"
     CDS             2769..3680
                     /locus_tag="ACG8OO_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9044701.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(3936..4853)
                     /gene="rnhC"
                     /locus_tag="ACG8OO_000775"
     CDS             complement(3936..4853)
                     /gene="rnhC"
                     /locus_tag="ACG8OO_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFP9044702.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5055..5492
                     /gene="zapA"
                     /locus_tag="ACG8OO_000776"
     CDS             5055..5492
                     /gene="zapA"
                     /locus_tag="ACG8OO_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9044703.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5550..6097
                     /locus_tag="ACG8OO_000777"
                     /pseudo
     CDS             5550..6097
                     /locus_tag="ACG8OO_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            6212..8572
                     /locus_tag="ACG8OO_000778"
     CDS             6212..8572
                     /locus_tag="ACG8OO_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFP9044704.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8659..8973
                     /gene="trxA"
                     /locus_tag="ACG8OO_000779"
     CDS             8659..8973
                     /gene="trxA"
                     /locus_tag="ACG8OO_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFP9044705.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9155..10933
                     /gene="uvrC"
                     /locus_tag="ACG8OO_000780"
     CDS             9155..10933
                     /gene="uvrC"
                     /locus_tag="ACG8OO_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9044706.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11078..11977
                     /locus_tag="ACG8OO_000781"
     CDS             11078..11977
                     /locus_tag="ACG8OO_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044707.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            11970..13232
                     /locus_tag="ACG8OO_000782"
     CDS             11970..13232
                     /locus_tag="ACG8OO_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9044708.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13395..15293
                     /locus_tag="ACG8OO_000783"
     CDS             13395..15293
                     /locus_tag="ACG8OO_000783"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFP9044709.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(15592..16311)
                     /locus_tag="ACG8OO_000784"
     CDS             complement(15592..16311)
                     /locus_tag="ACG8OO_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFP9044710.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    16501..16548
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16648..16980
                     /locus_tag="ACG8OO_000785"
     CDS             16648..16980
                     /locus_tag="ACG8OO_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044711.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            16967..18970
                     /locus_tag="ACG8OO_000786"
     CDS             16967..18970
                     /locus_tag="ACG8OO_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFP9044712.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            18989..19459
                     /locus_tag="ACG8OO_000787"
     CDS             18989..19459
                     /locus_tag="ACG8OO_000787"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFP9044713.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19480..20064
                     /locus_tag="ACG8OO_000788"
     CDS             19480..20064
                     /locus_tag="ACG8OO_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFP9044714.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20079..21065
                     /locus_tag="ACG8OO_000789"
     CDS             20079..21065
                     /locus_tag="ACG8OO_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFP9044715.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            21055..21366
                     /locus_tag="ACG8OO_000790"
     CDS             21055..21366
                     /locus_tag="ACG8OO_000790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFP9044716.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21388..23169
                     /locus_tag="ACG8OO_000791"
     CDS             21388..23169
                     /locus_tag="ACG8OO_000791"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFP9044717.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23162..24538
                     /locus_tag="ACG8OO_000792"
     CDS             23162..24538
                     /locus_tag="ACG8OO_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFP9044718.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24545..25180
                     /locus_tag="ACG8OO_000793"
     CDS             24545..25180
                     /locus_tag="ACG8OO_000793"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFP9044719.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25210..26565
                     /locus_tag="ACG8OO_000794"
     CDS             25210..26565
                     /locus_tag="ACG8OO_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9044720.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26757..28151)
                     /locus_tag="ACG8OO_000795"
     CDS             complement(26757..28151)
                     /locus_tag="ACG8OO_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9044721.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28243..29214
                     /locus_tag="ACG8OO_000796"
     CDS             28243..29214
                     /locus_tag="ACG8OO_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFP9044722.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29211..29993
                     /locus_tag="ACG8OO_000797"
     CDS             29211..29993
                     /locus_tag="ACG8OO_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFP9044723.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30162..30533)
                     /locus_tag="ACG8OO_000798"
     CDS             complement(30162..30533)
                     /locus_tag="ACG8OO_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9044724.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30667..31110
                     /locus_tag="ACG8OO_000799"
     CDS             30667..31110
                     /locus_tag="ACG8OO_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9044725.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31153..31962)
                     /gene="yidA"
                     /locus_tag="ACG8OO_000800"
     CDS             complement(31153..31962)
                     /gene="yidA"
                     /locus_tag="ACG8OO_000800"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9044726.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(31981..33348)
                     /locus_tag="ACG8OO_000801"
     CDS             complement(31981..33348)
                     /locus_tag="ACG8OO_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9044727.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33332..34162)
                     /locus_tag="ACG8OO_000802"
     CDS             complement(33332..34162)
                     /locus_tag="ACG8OO_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFP9044728.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34325..34762
                     /locus_tag="ACG8OO_000803"
     CDS             34325..34762
                     /locus_tag="ACG8OO_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFP9044729.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            34838..35458
                     /gene="rpoE"
                     /locus_tag="ACG8OO_000804"
     CDS             34838..35458
                     /gene="rpoE"
                     /locus_tag="ACG8OO_000804"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFP9044730.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        11598 a         6048 c         7646 g        10235 t
ORIGIN      
        1 ggaggaggag ttaaatgttt ttagcattaa atgaaattat gcattcaaag ttacgttatg
       61 cgttagttgc aggggtgatg tttttgatcg cctacctggt attttttttg actgggttag
      121 cttatggctt agctcaagat aatcgaacag cagtcgacaa gtgggaggca gattcgattg
      181 tgctttcgaa agatgcaaat tcaaatctag ggatgtccat gattactaag aagatagcag
      241 aagaagtcga aggtgggaaa gtggcgtatc ttgcccagac acctggagtc gtgactagta
      301 aagacagtac agaagaagga aaaatcaatg ttagcttctt cggtatcgat aaaaatcaat
      361 tcatcatgcc caatcttgtt gaaggaaaag cttttgataa cgatgatgag gcagtaggcg
      421 atattagttt gaaagaagaa tatggtctag ccgtaggaga tacagttaag ttatctggta
      481 gtgacaagac gtttaaacta actggcttta cggatcatgc caaattcaat gtctcaccag
      541 ttctctatac aaccatcaat gcttaccaag agatccgctt cgaaaaagaa gatacaagtg
      601 aaaatgcacg gattaacgca atagttgtca gaggtaaaat cagtaattta cctgaagatt
      661 tagaacagat caaaatttct aaattcatca atgaattacc tggatataat gcgcaagtat
      721 tgacttttgg atttatgatc ggatttttga tcgttattgc ggctattgtg atcgggattt
      781 tcatttatgt actgacgatg caaaagatta atatctttgg tgtaatgaaa gcacaaggga
      841 tcacaggagg atttattgcg cgttctgtag tggcacaaac attcatttta tcatttgttg
      901 gtattcttct tggtctatta ggaacagtgg gaacatccct tgttctacct gatgcggttc
      961 cattccaaag caattggctg ttttttggcg tcattagctt gttgatgctg gtagtagctg
     1021 tgttaggcgc actattttct gttcggacaa tcgtaaaaat cgatccgttg aaggcaattg
     1081 gataagaagg aggaagagca tgaaagcaat agaatttaaa gaagtagaaa aaagtttcca
     1141 agatggcgac caagtagtta aagcattgaa agaaacaacc atgagtatcg ataaagggga
     1201 atttgttgca atcatcggtc cttctggttc aggtaaaagt acttttttga ctttggcagg
     1261 cggattgcag acgcctagca gtggctctgt atttatcaat gatcaagaat tcagtaatca
     1321 aaaagaaagt gaacgcgtta aactacgttt taaagaaatt ggctttattt tgcaagcttc
     1381 caatctggtt ccttttctga cagtaaaaaa gcagttatta ttagtagata agatcaaaca
     1441 tgaacatcgt caaaaagaag caaaagaatt atttgagcag cttggcgtgg ataagttgat
     1501 caacaagtat ccagaagaac tatctggtgg agaacggcaa agggtcgcaa tcgctcgtgc
     1561 attgtacaat gatccgtcca ttattctagc agatgaacca actgctagtt tggattcaga
     1621 aaaggcacga gaagtagtcg atatcttggc aaaagaatca aaagacaaaa ataaagcaac
     1681 gatcatggtt actcacgata cacggctgat tgacaaatgc gatagaatat ttatgatgga
     1741 agatggtgta ttgaaagaaa aagaataacc aaaaagcagg tggaaaaatg gtccatcgat
     1801 acaacaaaga tacacagcaa aaaattttga aagaagcgac tgacttgttt atgtccaagg
     1861 gatatctcgg cacttcaaca agagagatcg cacaaaaagt tgggatcacc caacccaatc
     1921 tctatcatta tttcggtgat aaagaaaaac tatatacggc agttttagaa aaccacttaa
     1981 aagatgttgg aaaagctttg agagaaatcg ttcaaacaag cgaaacggga tttcaagtaa
     2041 ccttgacaaa aatggcgcag tttttgattg agacacatct ggtcgatttg tttctgatgc
     2101 ttcacgatct tgaaagcaat ctttcaaagg aaacaagaga tcatcttttc tttctatgga
     2161 agaaaaatta tcgtgaacct tttgaagaaa tcttttcagc aaaccaatct gtgctaagaa
     2221 atgggatttc aaaagaaatc gcagcaaggc attttttctt agtgttagca ccttacatca
     2281 ctcggtcgaa aagacctgtg gaagaaccgc taaatgtggc gcaattgatc gatttatatt
     2341 tgaatggtgt attaggagaa gcgtaaagct gttatatagg gagaaaaata gttaggtgaa
     2401 gaagcaagat atgacgaaga tcgttatatc ttgttttttt tatataggta gtttttcata
     2461 aatacaaatt tgttattctc tcatgattat cagggtatat ttttatttat tatgtcggtg
     2521 ttccaggaag tgttttaatt taataacgaa gaaattaatt gtttgtagaa tgaaggaaac
     2581 tatttattgg gtagatgaat caccacgaat agtcaaaagg agatttcagg atatgccaaa
     2641 aatgaaaaaa atcattttag cagtaatgtt attttctacg gttatatctt ttacagcccc
     2701 ttttttgcgt acttaagctg attttaagtt ttgaccctta gtctggatct acgaggtgag
     2761 agtcaagaat gaaaaaaata attggagctt ttgtatttgc aatcattatc attattatct
     2821 gttttcaaat agatgttttt ggtcaaaaaa agatagttgg acaaacggtc agcagtacga
     2881 acagtacggt tacaaagaca aaggaatcat ttgatgatac agaacaagag acattaatca
     2941 aaatcggaaa aaatgaccgt acactatata cagatgactc gctaactacc ccacttgcaa
     3001 ctataaacgg cggagagctg gcggagtttc tttctgaaac aaaaaatgct tatcagatca
     3061 agacaaatga tggctatact ggatatttag ccctcgaaga tggaaccaaa gtcacaaaga
     3121 atatacaaac aaaaccggaa gagctatctg atgcagtgat tgtattagat cctgggcatg
     3181 gcggaaatga tactggggca ttaagcaatg atgaacaaac agaagaaaaa gatataacat
     3241 taagtacagc cataaaagta aaaaaggcgc tagaggatgc tggagcaacg gtttatttga
     3301 cccatactac agatgaatta gtacagcttg gagatatatg cgactacagt gaagaaaaaa
     3361 aggcagatgt ctttatcagt ttacacgcag actctacgga atatgcgaat gaagcaactg
     3421 gaatcacgac atattattac tatggacaag aggaaacatt agcacagaca attgctgact
     3481 ccttcactga tctgcctctg gattcaagag gaatctccac tgggaactat caagttcttc
     3541 gagaaaatct tcaaccttct attttaatcg agatgggtta tatgaataat gatagtgatt
     3601 taaaggaatt agtaacagac agttatcagc agcagattgc tgcaagtttg acacaagcat
     3661 tgaccactta ttttcagtaa taaataagcc cagccaaaca aaaatagctc ctcatatttt
     3721 tcgtttggcc gggcttatta tcgcagatgc aagcttttga acaccgttta aggaagaggt
     3781 tgtgagcctg ccgtttaact ccgcgtcaca aggagcaatt tcaaaaaaca gcttctcatg
     3841 tttgatgact attgtgactt gtgaccgagg agttggcatg agaacaccgt ttattcggaa
     3901 caagggactg tgacatgact tttgtcacag ttccattatt tttttagcag cttcaaagct
     3961 ttttctgtat tggcaaaatg aagtttggca tatttttcaa gcataggcat gccgcctttc
     4021 tccaatagtt ttgccgctac ttggtcagat ttcgttccag ctcctgatgg aagggtgatt
     4081 ccagcttcag ctgatgcttt gtgtaactct tccaaaaagc tggcacgact gatgatagaa
     4141 gcagcagcaa cagctaaatg atattgttct cctttagtaa cgaagtacag tttttcttgt
     4201 aattgatttt tttctaaacg cgcatatttt ttaaaatttg cttccggcgt aaactgatcg
     4261 atcaaaatcg cttctggttt ttctggctcg atttttttta gcagcaaatg gatcgcttga
     4321 ttgtgcaaag cgactttcat ccgaacggca ttgtattttg gttggatctc attgtatttt
     4381 tctggagata agacaagcag tttatagggg atggtttttt ttaagatgtc agcaagctgg
     4441 atgatttggc tatcttttaa ctcttttgaa tctctaacac caagtttacg taattctgca
     4501 agctgatcac gtttcacata agcagcacat actgttagcg gaccaaaata gctgccattt
     4561 ccgacctcat cactacctaa aacggataat tgactgatat tttttggcaa agaagaggct
     4621 gtttttgctg gcttggcttg tttatcctgt gcctcccccc atttatcagc ttctattgct
     4681 gcattcgtac cttgaaacat cacttttccg gatgtataag cggtaatggt cgtcccgtct
     4741 ttttttgcag caaagctagt ataaggaatc tgtttattta acaaataaga gcgatagtat
     4801 gctttcattt gactaagttg gtcgtttgtt gctttgataa tgacgttgct cataaaattc
     4861 tcctcgtttt tatacataaa actataccgc aactcacgac ttttcgtttt ctaagagatt
     4921 gcgagacata gcattttttt aaagtttatc atataatagt taagtatgtg aaaaatattt
     4981 gtggagaacg gtgaaagaac caacttcatg gtacactaaa taaaactttc atcaaatagg
     5041 aggaaatgat tcgtatggcg cacgaaaaaa cacgctacaa agcagtgatt gccaaccaaa
     5101 catatactat tatcggacgg gaaaccaagc atcatatgga tatcgtaacg aagctgatca
     5161 atgaacagtt agcagaattg aaacaattat caccacaaat ggataatgaa caagcagcaa
     5221 tcttgatggc tgtcaatgcg ctttcggatc aattgaagaa acaagaaaga atcttggagc
     5281 tggaagaaga aacagcagaa ttaaagaaaa aaatgatcaa atttacagag cttgaaaatc
     5341 gtgtgaaacg cattgaagca atcgagaacg aagctcggga agtattgaaa gaaaatggtc
     5401 aagcagatca taccattaaa aatcatgtgg aagcacaaca gattttaaac gagaaacgaa
     5461 aaggacaaat taaacaaaaa tcttctaatt aagatccttt tatcaaaata agaaaaattt
     5521 aaaaaagtta gaagagaaag gaacgtagca tgctgagttt gctgatttta tttttattgc
     5581 taattgcgtt tttttctgga gcacgtcgcg ggtttgcttt gcaaatcata ttcgcgattg
     5641 gatatgtatt gtcatttatt gctgcccagc atttttacaa accattagca agtcatttgg
     5701 aattatacat tccataccca gctgtcaccc caacatcaaa gttagcattt tttgatcagg
     5761 tatttgcttt tcacctagat gaagcttttt atgcaggaac tgcttttctg attattttgc
     5821 taattggctg gctgcttacc cgatttgtgg gggtttttgt ccatggattg acgtatgtgc
     5881 cgatcttgag acaggtagac tggattgcag gaggcatcct gtcactaatc atggcttacg
     5941 ttactatatt tttgattttg cagttacttg cctttgttcc attagtgtgg tccaaaatca
     6001 attcggcgag catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa
     6061 gatgtatgag ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt
     6121 tcgttttttg tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct
     6181 cttttttcat agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga
     6241 atttgaaaaa gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga
     6301 actggcagaa ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac
     6361 tgaggatgga ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aacttgaaaa
     6421 tatccgtccc catatgaaac ggatcgagat cggagcagac ttgaatggag tggagcttgc
     6481 acaagtttcc agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc
     6541 tgacagtgaa atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc
     6601 tgttttgagc cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc
     6661 ttctcctgag ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga
     6721 acagttagat gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac
     6781 catgcgaaat gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg
     6841 tgtagtccac gatcaaagtg cttcaggaca gactttattt attgaaccaa aacaagtggt
     6901 ggatttgaat aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat
     6961 tttaagtgaa ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatgctta
     7021 tgtgattggt aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc
     7081 catcgtgccg ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact
     7141 gatcgatcag gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat
     7201 cgtcattaca ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt
     7261 acaactgatg gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat
     7321 ttttgaggaa gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac
     7381 cttttcttct catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct
     7441 ggtattattt gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat
     7501 cgcaatctta gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc
     7561 tgaattgaaa gtttatggat acaatcgggc aaatacgatt aatgccagca tggagttcga
     7621 tgtggataca ctaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc
     7681 gtttgaaatc tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt
     7741 gatgaatgat gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa
     7801 aatggcagag accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca
     7861 cgatgatttg aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa
     7921 agcaaagaaa aaagcaaacg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat
     7981 tgctgatatt cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt
     8041 gatcgatgca aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa
     8101 agtattgaag aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac
     8161 gacttacggc caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat
     8221 cggtatatta aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga
     8281 gccgacacaa cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca
     8341 attggacctt cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga
     8401 ttctgccatt ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc
     8461 acttcgcaag ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt
     8521 tgcaccggca aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact
     8581 aaaggtaagc agatggttcg ctatccaagt atagaaatgc tatactaatc atagatttta
     8641 aaaatggagg tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca
     8701 gataaaggac ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa
     8761 gcaccgattt tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa
     8821 atggatgtgg atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca
     8881 ttaatgttga aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa
     8941 caactacgtc aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg
     9001 tgggactgaa tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt
     9061 aatatttttt gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat
     9121 aataacttta attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt
     9181 agctttactt cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat
     9241 ttatgtcggc aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggcagcca
     9301 taatacgaaa acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga
     9361 atcgaatatc gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata
     9421 caacattatg ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata
     9481 tccccgtttg gtgattactc gtaaagtact aaaagacaaa gcactgtatt ttggaccata
     9541 tcccgatgtc ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag
     9601 aaaatgcaaa cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgtct
     9661 gtgtccttat tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa
     9721 aaagttttta aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa
     9781 agctgctgaa aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat
     9841 tgagacaatc atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt
     9901 tggttatgcg attgacaaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa
     9961 gctaattgaa cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt
    10021 gacttacctc ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat
    10081 tcctgatacg atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc
    10141 aaaacgagga gaaaagaaaa aattagttga attggcgaat aagaatgcct cagttgcttt
    10201 gaatgaacgg tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg
    10261 tttaggaaac gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa
    10321 tatcatgggg acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag
    10381 aaaagaatat cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat
    10441 gagagaagtc atctatcgca gatattcacg agtgattcgc gaagggttgc ctttacctga
    10501 tttgatcttg atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa
    10561 tcaattagga atcgatattc ctattgctgg catggcaaaa aacgataaac ataaaacaaa
    10621 tgagttgcta ttcggtatag atttggctgt agtaccgctt gagcgaaatt caccagagtt
    10681 tttcttactc cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca
    10741 gctacgcagt aaaaacagtt ttgcttcccg attagatgaa attgaaaggt taggaccaaa
    10801 acgaaaaaaa atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac
    10861 agaagaatta caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt
    10921 acatcaggaa tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt
    10981 acaacaaaaa gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct
    11041 aaggatatat gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt
    11101 taaaactttt gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa
    11161 aatcatgggg ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt
    11221 ggattttttg acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa
    11281 agattatgat gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat
    11341 tcaagaccaa ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc
    11401 aaaaatcgat ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa
    11461 atctttgtct aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc
    11521 taaattgatc attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct
    11581 aaaagatgga atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa
    11641 tatggataat gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt
    11701 tttgaatgga aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga
    11761 atctggttta agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca
    11821 tgaagacaat acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac
    11881 tcgtgcgact cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga
    11941 gattttcaaa ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg
    12001 tctatttaaa aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca
    12061 tgctgggtat catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa
    12121 tcggcgtatt ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg
    12181 attttgagtt caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa
    12241 aaatcgatgc gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata
    12301 gcgaaaattc attaggacaa agtacacaac tactgataca acaacaactg actggcttgc
    12361 aatcgatgct tcgagcaagt caattaggca tttcaccaga agaagtagct agcttgagcc
    12421 aacaagcaag tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagttg
    12481 gagaagataa ctcgacagtc caatatgtcg taagttatgt agcaacgatt gtcttgttca
    12541 ttattatttt gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc
    12601 ggatcatgga agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaactaa
    12661 caggtgtttt acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag
    12721 gatacagtca attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg
    12781 ataacttgat tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga
    12841 ttttttctgt tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa
    12901 aagcaatttt gccagtgact tatctatctc taggcggata tatgcttgga ttgatactag
    12961 gagcttcaga tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt
    13021 catatattat gccggttcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt
    13081 ccttgttggt acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca
    13141 aatcgaatgt actcgtgtat agcgaaggcg gattatggag ttcattgaaa caatcgattt
    13201 caattatgag aaatgaacga aaaaaagcat aaaatataga aaaatataga aggaagctgg
    13261 acatttattg ttcccagctt cttttttgta tagacctatt tctatttttt tattatattg
    13321 gatatatata ttcattgaaa atagagcata acaactttat aatgttgagt ataccaattt
    13381 ttggaggcgt tttaatggac cgctatttag atttactttc agagaagttc cctacaacgg
    13441 aagatgtcat cacggaaatc atcaatctcg aagcaatttt gcagcttcca aaaggaacag
    13501 agttattttt gagcgatata catggcgaat ttcctgcttt tgatcatatt ctgcgtatcg
    13561 gttcaggaaa tcttaaagaa aagatcaaag accttttctc tgaacaaatg acggaaacgg
    13621 agatcagcca attcactatt tttacggctt atccagaata tgtgttgacg actgattggt
    13681 acaaaaaaca agataaatcc cagctgatcc atcgtttgat cgatctttta cggtttacga
    13741 cagtcaaata cactcgatca aaagttcgaa aaggattgcc gaaagaatat agctatatca
    13801 ttgaggaact cttgtatatc gatgatcgta tcaacgggaa aaaagactat gtaaaaaaaa
    13861 tcattgaaca acttgtcttg atgaaggaag aagaaaaatt tttaacaaaa cttgcgcaga
    13921 cgatccaaaa atctgtcatc gatcatttgc atatcgtggg tgatattttt gatcgaggaa
    13981 ctcaagcagc caaagtaatg gatcgaatca tggatttttc ctctgtagat atccagtggg
    14041 ggaatcatga tattttgtgg attggtgcgt attgcggctc tgaggcatgt ttattgacgc
    14101 ttttacggat tgccgcacgc tacaactatt tatttgaatt agagaaagaa tatggattaa
    14161 atttacgtcc attattttct tttgcccatc aaacctatca gaagaatcca gtatttaccc
    14221 ctaaaaacag gaaagatcta tctgaaagag agcaagaaga attagaaaag atccatcaag
    14281 cactttctat cctccaattc aaatcagaac atcagttgtt gaatcgacga cctgaattta
    14341 aaatggacga tcgtaaattg ttggaaaaaa tcaactatga ggaatcaacg atcgttttag
    14401 aaggtcagct ctatgagttg aaagggactt gttttcaaac gatccagcca gatgatccta
    14461 aaaaattctt ggaagaggaa caaaaagtga tcgatagttt gatgtattca ttccagcact
    14521 cgttaagaat gcaaaaacac atgacgtttt tgatcgaaaa agggggaatg tatctcacga
    14581 acaatcagca tgtactgttc catggctgta tacctgtaga tgaaaagggg cagctcttag
    14641 catttgaatt agaccaatct tatcgcggaa aagaactgct gggatttttt gaaaaacaga
    14701 taactgaaag cgcaaaagat ttgactgcaa atgaagattt ggcgaccgat ttgatttggt
    14761 atgcgtggag cgggccattt tcgcctttat tcggcaagag taaaatggca acttttgagc
    14821 gctattttct cacagagcct catacgcacg tcgaaaaaag caatccctat tatcacttgc
    14881 gagatgaaga gtggttcagt gaaaaaattt tggcagaatt cgatgcaaaa gaagaaggtt
    14941 cagcaatcat caatggacac acgccagtaa aagtaaaaaa aggagagtca ccgatcaaag
    15001 caaacggaaa agcatttgtt attgacggag gtctatccaa agcttatcaa aaaacaaccg
    15061 ggattgctgg ctactcatta ctatgcaatt cttacggttt ccaaatcgtg acacattatc
    15121 cgttcttgcc tattgaacaa caatttgcaa aaaaatctga tcaaacgaat gtaaaaaaag
    15181 tgatcgagca gcagctccca cgcaaactta atcggaatac gacaaaagga atggaattac
    15241 agcaacagat tactcaactc caaaggctgt tggattatcg aaaggacgat tgagaaaaat
    15301 gctagccaac attgctcaat agactaaaaa gcgactgcaa caaaaggcgt gtcgcagtct
    15361 ccttcggaat tagctcaaaa aacggtaaaa tattagaagc tatttttctg tttatttctc
    15421 taatactcag gtcagggcgc tgatttcacg acctcttcct taaacggtgt tctcctgcca
    15481 actcctcggt cacaagtcac aatatccaac aaacatgaga agctgttttt tgaaattgct
    15541 ccttgtgact cagagttgaa cggcaggctc acgacctaat cagaaaaacg attaaaattc
    15601 attcacaaag aaatcgaata aacgctgttc agatgaatct acacgatgag aaagttccgg
    15661 atgccattgg acaccaagga ttttgtgcat attatcttta ctttggactg cttcaatgag
    15721 tccatcgttg gaaaaggcga tggcttttaa caaaggagaa agatctttga ctgcctgatg
    15781 atgataggaa ttcacaaagt atgaatcagg gagaaggtca cttaataaac tgtccgaaac
    15841 gattttgact tcgtgagttg caaactgcgg ggcagtgggc tgttgttcgt gttttacgga
    15901 ccattttgga tataatgaaa gatcttgata aagtgttccc tccaacacga cattcaatag
    15961 ttgaagtcct cggcagactg caaaaatcgg ttttctttgt ttcaatgctt ccaagatcaa
    16021 tgcctgttcg aattggtctc gttgaatatt tgtttccgtc agttttggat gagggtcttc
    16081 catgtaaagt tgcggactaa catcctgacc gcctgcaaga atgagtttgt cgattttctc
    16141 aattagttga ggcgctgaat ctggagacat gactggcaga ataagaggta atccttgagc
    16201 atctaaaact gcatccacaa atccctgcgg tgtatagctt acttgatttc cttggaaagt
    16261 atctgtcgct cgaatcagtt gattcccggc aatgccgatg attggtttca tttttattcc
    16321 ccctaaagta gactaattgt aacaaggatt taaaaaaatt taaactatgt aagacttgcc
    16381 cctatactta tctgttcttg ctgtgttact atctgtaaag agaattttcg tgtgaaattc
    16441 atgacgatag ttgaaaatat tgcttaattt gttataataa aaagtaagca gaaataaaaa
    16501 ggttgggcgt aagcttcaag gatctagttc ttcaaggggt gagaaggaca ttttattgaa
    16561 ggggcctgcc tatactaagt tatctttaga ggtacatact ttcaatgaaa aaagaaagca
    16621 tgtacctctt ttttaaatcg aaaggaagtg aagcaagtgt tagaagcttt ggaacaaatc
    16681 cgaaaagcag aagaagaaaa tgagaagcgt gctcgaaccc tacggcagca gttgaagacc
    16741 tatgcagaag aaaaagagca agaattggag ataatccaaa cgaacagtcg gcaacagctg
    16801 aaacagttgg tagaagaaaa agaacaattc gaaaagcaag aattagaaaa agaaaaaatg
    16861 aaattgaaag cagaagcaga caaggtgcaa gcaactttga aagaccaata tcaaaagcat
    16921 cagagtcaag caatcggtgc aatcattgaa agggtgaaag aaacttatgg ccgtcactaa
    16981 gatggaaaaa gtgaccctca tttcagataa aagaaatcag gagaggattt tacaagcagt
    17041 acaaggaatc caaaatgttg aaatccggga tctgtatcaa aatagaacaa acaaccagtg
    17101 ggtagaaaaa tattttccag ataccacgat tattgataaa gaagcaaaat taagcgcttt
    17161 aggcaatcgg ctgaaagaca tccaagaagc tatccaattc atacaacacc acggcgatag
    17221 ccgtcaaaag aaactccatt tgaaacgtca agaactttcc ttacaagact tggaagcaaa
    17281 ttattccgaa gaaaatttca tgaataaatt agaagaagtc cttgatttaa aagaaaagtg
    17341 ggaacagcta actgaaaaac gtgatcgttt agaagaagag gaagaatggc ttcttcattg
    17401 gcaacagctg gatattgctc caagtatgta tgcaagtaag acgaccattt ttgagatggg
    17461 gactgtgagt gcagcaaatt ttgaaagttt caaggaagaa ctaagtaagc ttgatgacgt
    17521 ttatatggaa gaaatagatt atacgccaaa acaagtttat ctttcgtatg ttgctttaaa
    17581 aaaatcagtt gctgctatag aagaaattgc tgggaaatac gggttcaata aagaaacgta
    17641 tccttatgaa caaaatccta aagaaaaatt gcaagaaacc aaacagcaat tatccattgt
    17701 tcacgagcag caaaaaaaga taacgacagc gttaggtcag tgcagcggct atatccaaga
    17761 atttgaatgg gtagaagaag tcacacttgc cgttgcagaa cgggaaaaag taaaagaacg
    17821 gtttatccat gccacttatc tgatcgtttt gcaaggttgg gtagatgcag aagaaaaaca
    17881 gaatttgatg catgtgttaa acgaaactgt atcagaagaa gatatatacg ttagtttcga
    17941 aacaccaaac tcagaagaga ttcagcagga agtccctact aaattgaaaa accatccgct
    18001 tatcgaacct tttgaattgc tgactgaaat gtacagtctg ccaaaatatg aagaagtaga
    18061 tccaacgcct tggatgacac cattttatct tgtcttcttc gggatgatgg tcgcagatgt
    18121 tggttacgga ttgctgatgc tcattgggac aatactagct caaaaactat ttgttttacc
    18181 ccgcgggatg aaacgtttcg taaaattctt tgagattctc tcggtaccat cgatcatctg
    18241 gggattgatc tacagttcat ttttcggaca ggcattacca aaagaactat ttggtatccg
    18301 cttgcctttc ccaatcctat ctacgacgga tgacgtcaat acgattttga ttttatccgt
    18361 gattttcggt ctgatacaga tcttggttgg attattcgtg gcagcaaagg aaaatatcag
    18421 gaggaaagat tacttgagtg ctgtcagtga tggttttgcc tggcaaggga tcctgattgg
    18481 gatcgttatt gcattgattg gtgtcatgtt gataaaaaat acagcatttg tttatcttgg
    18541 cgcggggcta tcgatcattg ctgctttatg tatcttaatc gtgccgatta tccaatcgcc
    18601 gtctaaagtc aagggtgctg ccaaaggagc gtataatctt tacggattga ctggttatat
    18661 cggtgattta gtcagttata cacgtttgat ggcactaggt atttcaggcg gaagtattgg
    18721 cgcggcattc aatatgctag ttgctttcat gccgccagcc gctcgattca gtgtagggat
    18781 ccttttgatc atcgctttac acgcactgaa tatgtttttg acactgttaa gtgcctatgt
    18841 ccatggtgca cgattgcagt atgtcgaatt ttttggaaaa ttttatacag gaggcggacg
    18901 tgcctttgac ccgctgaaaa cggcggaaaa atacgtcaac atcaatcata agaaaaaaaa
    18961 caataaataa ttggaggatt tttatacaat gatggattac ttaattagtc aaaatggtgg
    19021 aatggtattt gcagtattag cgatggcaac agcgacgatt ttttcaggga ttggttcagc
    19081 taaaggtgtt ggaatgacag gtgaggcagc agcagcgtta acaacaagtc aacccgaaaa
    19141 attcggacag gctttgattc tgcaattgct tccaggtacg caagggttgt atggattcgt
    19201 tatcgccttt ttgattttta tcaacttagg aaatgatatg tcagttgtac aaggattgaa
    19261 ctttctaggc gcttctctgc cgattgcttt tacaggtttg ttttcaggta tcgcccaagg
    19321 gaaagttgca gcagcaggta tccaaatttt agcgaaaaaa cctgaacatg ctacaaaagg
    19381 aatcattttt gctgcgatgg ttgaaacata tgcgatttta ggtttcgtta tttccttcct
    19441 attagtattg aatgcctaag cataaaggag gaaaagcaga tgaacgccat tgaaaaaatc
    19501 atctctcaga tgaatgaagc agcagagcaa gaaagagcgg cgttagagca agaagagcga
    19561 atgaagatcg atcaaaactt tgaacaaaaa cgtacgcaaa tcgaaacaga acatcaaaaa
    19621 caaaaagaaa aacaaatcga attacttgaa aaaaaatacc gtcagcttag gaatcgccaa
    19681 caagtagaag tacgacaaga aaatttgaat gcgaagcagg aatttttacg acgattgttt
    19741 gcagatgcaa tagctgaaat ggagaattgg gatgaatctg aacaaatcca gtttatcaaa
    19801 aattcattgt attcattacc tttgacaggc aaagtaacat tcattgctgg agaaaaatca
    19861 gcagcttatc tttctcagac attgctggat gaatggaaca atgaacttcc ctttatgatg
    19921 gttttaagtg atgaaactgt tgctgatcaa gcgggttttc tgataaacga tcaaggagtt
    19981 cagtataatt ttcttttcag cagccttgta caagatatcc aaggaacgat gagttttgag
    20041 attgccaatc aattattcga ataaggaggg atcagtgtat ggaataccac gaactaaatc
    20101 ccttgattcg cggaagagag cttgaacttt tgtccaatga gacatttgaa cggatgattc
    20161 aaacaaattc tgtcgaagca cttggtgaaa tattgaaatc cacgatttac agtccatata
    20221 tttatgacgg atatgaatat gacttcgaac agaatctttt aaaggagcag agccgtctat
    20281 ttcgctggtt aaaggaatca gcaccggagc ctgaaatcgt ttggatctat acgatgcgct
    20341 atactttcca taatctgaag gttttgacaa aagccgagat gacaaatcaa aatctggacc
    20401 atttgtatat cgacgatgga ttttattcat tagaaacctt gaaggatgcc attcatacgc
    20461 aagtttcaaa tgaactccct gaaagcatta tggacagtat acgagaagta cgtgattatt
    20521 ttgaagagtc taccattctt cagggaattg acgtgattta tgatcgacag tttttgacag
    20581 agcagcgacg gcttgccgat aagctaggat atcaagaatt gctgacagaa gtcattgctt
    20641 ttattgactt gaccaatatc acaacgatgg ctcgaggaat attgcagaaa agaacatatg
    20701 ggttcatgac gacggtactt tccagctcgg gaagtatcga aaaagaagta tttttgaact
    20761 ttgtagataa agatatggaa gattacacga attttctatt agagacagat tatgctgatt
    20821 ttctgcgccc tgtcatccgt gagggaaatc ttgatctcgt aaagcttgaa cagttgaaag
    20881 atgattatct aagttcattg tatcaagaag cacaaaccca ggctttcgga ccactgccac
    20941 tactagcctt tttgaacgca aaagaggtag aaagcaaaaa ccttcgttta ctggttatcg
    21001 gcaaaagaag ccatttcgaa ccagaaacaa tcagaaagcg ggtgagacaa gtctatgacg
    21061 cataagatcg gtgtagttgg cgacaaagat tctgttttgc cattcaaatt gtttggcttt
    21121 gatgttcgtt atggtacaac caagcaaacc gttcgtcagg caattgaaga aatggcaaag
    21181 aatgagtatg gcgtgattta tgtcacagag caatgtgcag cattgagccc agaaacgatc
    21241 gaacgttaca aggaacaaat gatacctgct atcgtattga ttcccaatca tcaaggatca
    21301 ttagggatag ggctggaaga aattcaaaaa aacgtagaaa aagcagtagg tcaaaatatc
    21361 ttatagtatg aaaaggagat agaaattttg caaattggaa aaatcataaa agtctccgga
    21421 cctcttgtaa tggctgagaa catgtctgat gccagtatcc aagacatgtg tctagtggga
    21481 gatctaggag ttatcggaga aattatcgag atgcgtggcg atgtggcgtc tattcaagta
    21541 tatgaagaga cttctggaat tggtccaggc gaaccggttc gctctactgg tgaagcttta
    21601 tctgttgaat taggaccagg gatcatttca caaatgtttg acggaatcca acggccatta
    21661 gataccttta tggaaatcac acaaagtaat ttcttagggc gcggagttca attgcccgca
    21721 ttggaccatg agaaaaaatg gtggttcgaa ccaacagtag aagccggaga aacggtgagt
    21781 gctggagatg ttatcgggat cgtagaagaa acaaaagtta taaaacataa aatcatggtg
    21841 ccaaatggga taaaaggaac gatcaaaaca atcgaaaaag gttcattcac tattgatgaa
    21901 acagtatgtg tcattgaaac cgaaaatggt gacaaaaatt tgacgatgct tcaaaaatgg
    21961 ccagtaagac gtagtcggcc aatcaaagaa aaattaaacc cggatgcacc catgatcact
    22021 ggacagcgag tgattgatac attcttccca gtgacaaaag gaggagcagc agctgtacct
    22081 ggtccttttg gtgcagggaa aacagtcgtt cagcatcaaa ttgcaaaatg ggccgatgtg
    22141 gacatggttg tctatgtggg atgtggcgag cgtggaaatg agatgacgga tgttttaaat
    22201 gaattccctg aattgattga ccctaataca ggagaatcgt tgatggaacg tacggtattg
    22261 attgctaata catcaaacat gccagtagca gctcgagaag cttctatcta tacgggaatc
    22321 acaattgcgg aatatttccg tgacatgggt tatgacgttg ctatcatggc agactccact
    22381 tcacgatggg cagaagcact tcgtgagatg agcggacgtt tggaagaaat gcctggtgat
    22441 gaaggttatc cagcttatct tggttcacgg cttgcggaat actatgaacg atctggaaga
    22501 gtcattgcat taggatcaga aggtcgcgaa ggaagtataa ccgcaatcag tgctgtatcc
    22561 ccgtctggag gggatatttc ggaaccagtc acacaaaata cattgcgggt cgtaaaagta
    22621 ttctggggtc ttgattccag tttagcccaa aaaagacatt ttccttcgat cgattggata
    22681 caaagctatt ctctttattc aacagaagta gggaagtata tggatcatat ccttcaagct
    22741 gactgggcat ccatggtcac cgagggcatg cgtattttgc aggaagaaca gcagctgaat
    22801 gaaatcgtgc gtttggtagg gattgattcc ctttcagata atgaccgtct gacattagaa
    22861 gtggcaaaat ccattcgtga agattatttg cagcaaaatg cttttgatga tgtcgataca
    22921 ttcacttcaa gagagaaaca attcaaaatg ttgaagatga tcttgacctt tggtgaagaa
    22981 ggacgccgtg cgttgtcgtt aggtgcttat ttaagtgaga tcatggacgg taccgtatcg
    23041 attcgtgaaa ggatcggacg aagcaaatat atcccggaaa cagaattaga aaaactagat
    23101 caaattattg gtgaaatcaa gcaaacgatg caaacgatcg tttcagaagg aggcatgagc
    23161 aatgattaaa gaatatcgta cgatcggtga agtcgtcggt ccattgatgg cggttgaaaa
    23221 agttgctggt gtcaaatatg aagaattaat cgaagtccgt atgcaaaatg gcgagattcg
    23281 tcgtggacaa gtattggaag tccaagaaga taaagcaatg gtccaaatat tcgaaggaac
    23341 aagtggtatc aatttacgtg actcttctgt acgattttta ggccatcctt tagaactagg
    23401 tgtatcggaa gacatgatcg gtcgagtatt cgatggtttg ggacgaccga aagataacgg
    23461 accagagatc ctgcctgaaa agcatttgga tattaacgga gaagtaatca atccagtagc
    23521 tcgggattat ccagatgagt ttatccaaac aggtatctct gcaatcgatc atttgaatac
    23581 attagttcgt gggcaaaaat taccagtatt ttctggctct ggtcttcctc ataaagaatt
    23641 agctgctcag atcgcgcgac aagcaacggt actcgattca tcagatgatt tcgccgtagt
    23701 ctttgctgct attggaatca cttttgaaga agctgaattt ttcatggaag attttcgtca
    23761 aacgggcgca atcgaccgtt ctgtcatgtt tatgaattta gcaaacgacc ctgcaatcga
    23821 acgtatcgct actcctcgta tggctttgac tgctgcagaa tatcttgctt atgaaaaggg
    23881 aatgcatgtt ttggttatca tgacagacat gaccaactat gcagaggcat tgcgagagat
    23941 ttctgcagca cggcgagaag ttccaggaag aagaggatat cctgggtatc tttacacgaa
    24001 cttagctact ttattcgaac gagcaggaag aatccgcgga ctaaaaggat cggttactca
    24061 aattccgatt ctaacaatgc cagaagacga taaaactcat ccaatccctg acttgactgg
    24121 ttatattaca gaaggacaga tcatcttgac tagagagctt tacaagagtg ggattcagcc
    24181 accaattgat gtactcccgt ctctttcgcg gttaaaagat aagggaactg gacccggaaa
    24241 gacaagagaa gaccatgcag ctacgatgaa tcagcttttt gcagcctatg cacaaggaaa
    24301 acaggcaaaa gaattagccg tagttttagg tgaatctgct ttatctgatg tagacaagat
    24361 ctatgcgaag tttgctgagc ggtttgaaaa agaatatgtc aatcaaggat tctacacgaa
    24421 ccgttcgatt actgaaacac ttgatctagg gtgggaactg ctatcgatgt tgccaagaac
    24481 cgaattaaaa cgaatcaaag acgacatgct ggacaaatat ctgccagaag ggaagtgagt
    24541 tctgatggca cgattgaatg tgaatccgac ccgcatggaa ttgactcgat tgaaaaaaca
    24601 gttgacaact gccacaagag ggcataaatt attaaaagat aagcaagatg aactgatgcg
    24661 tcaattcatc ctgttgattc gaaagaacaa ccagctgcgt caagaaatgg aagagtctat
    24721 gacagaagca atgtctgatt ttgtattggc aaatgcttcg atcacggaac ctttcatcga
    24781 agaattattt gttttgccgg cggaaaacgt tgatttgtct atcgtagaca aaaatatcat
    24841 gagtgtaaaa gtacctgtga tgaattttga atatgatgaa cagctgacag acacaccttt
    24901 ggaatacggg tatttgaatt ccagtgccga actggatcgg tcaatcgatc ggtttactac
    24961 cctgcttcca aaacttttgg aactgacaga aatcgaaaaa acttgtcaac tgatggcgag
    25021 tgaaatcgaa aagacacgtc ggcgagtcaa tgcattagaa tatatgacga tccctcaatt
    25081 agaagaaacg atctattata ttcgtatgaa gctggaagaa aacgagcgcg cagaagtaac
    25141 aaggttgatc aaagtaaaaa atatgggtac cactgactaa gtggtatctt tttttaaaag
    25201 aggaaaacga tgattaaaca aagcaaacaa ataaagaaac gtttgccggc tgttcagctt
    25261 ctcgcattag gattttttct cctgatttta gttggcggcg gcctacttac cctaccattt
    25321 tttagtaata gtgggcatgg gacaaatttt atcgatgcgc ttttcactgc aacttctgct
    25381 gtctgtgtaa cgggattgac aacattgaat acagcagagc attggaacag tgctggtcaa
    25441 tttttgatca tgcttttgat tgagatcggc ggtcttggtt ttatgatggt tccgattcta
    25501 tgctttgcat tgataaagaa gaaagtcagt ctcagcacac gtattatatt aaaagaagcg
    25561 ctgaatctag aagaagtatc aggtgtgatg aagctcatgc tttacatcct aaaattggcc
    25621 tttgttatcc aattgattgg tacggttgct ttatcttttg tatttgtgcc tgaatttggc
    25681 tggttgaaag gtatctggta cagtttgttc catgcaatct ctagtttttg taatgcaggg
    25741 tttgatttgt taggagacag tttagccaat caccagcaaa atgtttattt gatcatggtg
    25801 gtatctgctt tgatcatttc tggtgggtta ggcttcatcg tatggcgtga tttactggaa
    25861 tatcatcaga aaaagaagat gacgattcat tcaaaaatcg cattaaccgt tacaggtacc
    25921 cttttattag gcggattcgt tgtgttcttt ttcacagagc gcaatggggc aacgctagtg
    25981 gaagggaata tgtttcaacg attggcaaac acctttttta tgagtgtgac acctagaacg
    26041 gctggatatt attcagttga ttacctccat atgagccatg caggtctgat tttaacgatg
    26101 tttttgatgt acatcggcgg aacatctggg tcgacggccg gcggattgaa aacaactacg
    26161 ttaggtgtgt tgctgatcca gatgcgttcg atgttgaaag gacgtacgag agcggaagca
    26221 tttggccgta ccatccgaca aggcgcagtg ttgcgggcac ttactttatt ttttgttacg
    26281 ttgacgttgt gtgtagtcgc catcatgatt ttatcggtga ctgaaacgat acctaaaact
    26341 tctggcatcg aatatattgc ttttgaggtc ttctctgcgt ttggtacagt aggtcttacg
    26401 atgggattga cgccagactt gactgcaata gggaaattga tcattatagc gcttatgtac
    26461 attggacgag ttggtgtatt gactgtggtc ttctctctga tgacaaaagc acaaagaaca
    26521 caatcaaaat acaaatatcc agaagaaagt gtcatgattg gataatttca tcttctgaca
    26581 gaaacctagt gatttagtaa aataagaaaa taaaataatt tttcttgctt cttggatact
    26641 ttaagtgcaa aatgttcgtt tattttttga ggcactaccc gttgttgaac ctcgggtagt
    26701 gcctcttttt tattgaaagg aatcaataag atttgattcc tttatttatc aataggttat
    26761 aattcatatt tttttatttt ttcttttacg atacttagtc cagaagctaa aatttcaagg
    26821 ttattagagg atagggaaat tcgaacgaat gcatctggtt gcgcacgtgt agtaaagcgt
    26881 ctggaatgat aaagtcggat accgttagca agaaagtcat tctcaattat cttagggtca
    26941 atctcaggat caactggaag aattcgatag taagggacac gattcgttgg tttttctact
    27001 ttgaaaagag aatcaaaaag ctgattggct ctttttgtaa gacttaattt agtttctaag
    27061 atcctatttg cttcatttga atggaggatt tgagtgatga cttccacatc taatccagaa
    27121 gttttcacat ttatattaaa aattgcttgt tctatcttgc tttggacatg tactggaact
    27181 acaagataag caactcgcaa tccagaacaa ataaattttg tcattccagc taagtagatt
    27241 gtttggttag gtaataaatt ttggaaagga ggaatgatcg tatgttgata gtaagttgtt
    27301 aaaaatgaat ggatatcatc ttcaataacc catagacctt ctttttcaat cacttcggct
    27361 aattgctctc gtcttttgtg agagatttga aaccctatag gattattaca agaaggcata
    27421 agaaagacac catgaatctt cttttttcta cattcctgaa tcaatatatc aggtcgcatt
    27481 ccttgctcgt caaaatcaat tggaacgatc tctaaatgat ataactgagc caattcaatg
    27541 aaattagcgt atgtatatcg gtctgttgct attctatcac ctggagagaa aagtgcagaa
    27601 agtgtgatag ccaatccatt ttgaacaccg gaaacaatag atagagattc taaggacgga
    27661 atgactcctt gagtttttaa ccaatttgct gcagtagtca attgaaaatt tgtgcctttg
    27721 ggatcttggt aattaagcaa accaacaaga tttgggttat tactagttga ttgaataaac
    27781 gggacaacga atttattaca ttcttcaaaa gaactaacca gaccaaaatc aatgatatgt
    27841 tcggttactt cattggtaga gatggtgatg gactcaaaga cattgggaga aataaatgtg
    27901 ccacttccaa tattcgtata cagcaaacct ttctcaattc catatttata agccttgcca
    27961 acggtagtaa aatttaaatc taaataatca gctagctctc tttgggaggg taactgagtg
    28021 tttttctgta atttattctt taaaatatct tgttctaatt gttgaatcaa cgatcgataa
    28081 taaggacgct tcaatttttg tttgtctggt cgccaagtta acgggtaatt gtcataagaa
    28141 ttaataggca ttttctccac tccttaaaat tgtataccat acaattttac cattgattgt
    28201 attgatttta gaagttaatc ttttgtgagg agggaattgt ctatgaaaat tattaatttt
    28261 gaggaagtag tagcttcttt tagttttgaa gaagcgattc aagtaatgaa aaaatgtttt
    28321 tttgattatg aaagtggtaa aatcagccaa aatcctcgat tggtggaaat tttacctgac
    28381 ggaaaaggca aaaatatgtt tgcgatgatg cctgcttatt tgggggagaa cagatatttt
    28441 ggcacaaaag tcattaccgc ttttccggat aatcatcagt atcatttacc ttctcattta
    28501 ggagagattt tcttgtttga tgctcaaaac ggactaccca aagcattgat aaacgcaaat
    28561 gcagtcacct ggattcgaac agctgctgta tctgcgctgg caacagatta cttagcaaat
    28621 ccgcatgcag aaacacttgc tttaattgga gctggtcaac aagcattgtc gcatttgact
    28681 gcgatttcta ccattcgtcc catcactaca gtgtttgttt acgatatttt agaagaaaga
    28741 cgaaatactt ttatcgctaa agctaaaaaa cagtatccta aaataacgtt tataaatgca
    28801 atatcagtag aagatgcagt acgtccagcg gaaatcattt gtacgttgac ctctagtaaa
    28861 gaggcatttc ttcaaaaaga gtgggtaagg gaagacgcac atatcaatgc tgtgggcaca
    28921 tttactcctg atacaagaga gataagtaca tcgttgatgg aaacttctca aatctatgta
    28981 gatgattact cagctgcctt aacagaaagc ggggacctgc ttatagccat ggcagaagga
    29041 gatttagagg aatcttgtat tgttggtaca ttaggcgaat tagtttctga gaaaaaaaca
    29101 ttttataaag gagaatcgag gccgacgatt ttcgacgcag ttggtctagc tattgaagat
    29161 ctttgttgtg cagaatatat ttataacaaa ttagagaaag gggctttaaa atgaacttag
    29221 attattacgt agttgatgca tttgcagatg aggtgttcaa aggaaatcca gcagcggttt
    29281 atgtgctaga agaatggctg ccagaaggta cgatgcaaaa aattgccatt gaaaacaatt
    29341 tatctgaaac agcatttaca gtgaagaaaa accaagaatt tgagcttaga tggtttacgc
    29401 cagatcgaga aatcgatctt tgtggtcacg cgacattagc aacagcattt gttttattta
    29461 actattacaa aatacctgat gaaacgatta aattttctac tcaaagcggt aatctatttg
    29521 tgacaagaat taaagattat tattacatgg attttcctag tattatgccg aaaaaagtgc
    29581 cgatattgtc tgaatatgaa gaagcgattg gggctaagat taaagaagcc tacttagcac
    29641 gtgatttatt tttcgtatta gaagatgaaa aaacggtggc taaattgcag ccagatttta
    29701 cagctatcaa aaattttgaa ttaggaatag gagtcatcgt gacagcagaa agtgtgcaaa
    29761 aagactttgt ttctagaacc ttctttccaa aattaacaat caatgaagat ccagtatgtg
    29821 gatcagcaca ttctaatctt attccttatt gggcagaaag aatgaataaa acaaaattgt
    29881 cagcttatca aatgtcatca aggggcgggg atctagaatg tgaattaaaa gaagatcgtg
    29941 tgataatagg aggaaaagca gttttgtttt ctaaaggtac ctcgtttatt tgatttcttt
    30001 caaatgaatt aaaaataaat tggtggagag tggcaacctt atagctacaa tggttgttaa
    30061 aaaaagacgc aaactttttc aatttaatga aaagtttgcg tctcatttta attattggtg
    30121 tttgtttttt attcaatcaa gagtaaaatg ccccatacta actatcctcg gatgacttcg
    30181 attttataac catcagggtc aatgacaaag tagtaagaag gcgcagtgcc aggtaatcct
    30241 tttaagtcag taatctcaag accggcagct ttatgttgct cgtgtaattt ttctagatca
    30301 tcagctgcta tagcgatatg accatagccg tctcctagat catacgcacc atgatcatag
    30361 ttgtaagtca actctaattc atagtcatcc ccaggaagtg tcaaataaac aagtgtaaat
    30421 ttgttttctg gaaaatcacg gcggcggctt tcttcaaaac caaatgcctt ttgataaaat
    30481 tcgattgatg cttcaagatc tttcactcga acacatgtgt gagccatttt catagattca
    30541 tctctccttt tccttttact caatcataac aaaaagaagt aaggaaatag ttgatttgtg
    30601 cttgttcatg agaaatttct catgtaaaat ggatagaaaa taaaactgtt aaggtggaaa
    30661 ctattcatgg ataaaccaat ttttggtgaa aaaaagcaat ttgctgatta tcaaacccgt
    30721 tatgcagcat atattgtcat agaaagagat cagcagattg cagtaatcga ggctccaaat
    30781 ggtgcttttt ttcttcctgg cggggaaatt gaaggagaag agacgaaaga agaagcgatt
    30841 gtccgtgagc tgatagaaga aatgggaatt gtggcagaaa tttcttctta tctgggagaa
    30901 gctgatgagt atttttactc gaattatcgt gcaacttatt attataatcc aggatatttc
    30961 tttgttgctg attcttggaa gcgtatagga gaaccaactg aaaagaccaa taaaatttgg
    31021 tgggtgagcc ctcaagaagc gcttgtgaaa ttgaaacgtg gtagccaccg atgggcagtc
    31081 gagagatgga tcgaaaaaaa taaaaactga aagaaagcga gtagatcagc tttctctcag
    31141 tttttttatt tattacagta catatttttt gattgcttca gctacaccgt gctcgtcgtt
    31201 tgatgaagtg atgacatttg ctgcttcttt gacaagagaa acggcatttc ccattgctac
    31261 tcctagacca gcatattcaa tcatggataa gtcgttctcg ttatctccga tagccatgat
    31321 ttcatcttga tcaataccaa ggtgctgtgc gagattcgca actgccgcac ctttacttgc
    31381 ttctttattt agtacttcga agtaataggg cgcacttttg accgttgtgt atttatcacg
    31441 gaaagtttgc ggtaaacgcg caattgctgc atctaaaatt tctggttcgt cgatcatcat
    31501 cattttgacg atgttcatgt ctggtgtcat ttcttcaggt gttcgatatt ttagcggcat
    31561 cttgactaaa cttgcttcgt ggatcgtata agcgctgata tctcgattag gcgtgtagat
    31621 cgctttgtca tcgatcgaat ggagatgact gccgacacgg cgtgccatca cttcgatctc
    31681 taggaaatct tcatgtgtca gtccgtgacg ggcgatgatt ttacctgatt tcgtttgttg
    31741 tacgagtgcc ccgttataag taataacgta atcattatct tgaaaaaggt tcaactcgtt
    31801 taactgttcc tggacacctg gtaagggacg gccagtgcat aaaacgatcg aaacaccttt
    31861 tgcaacagca gcggcaatcg cttcttttac ttcttttgtt acttctcttt tctcatttaa
    31921 taaagtacca tcgatgtcga tagcgactag tttgatagac ataatttcct ccaatatatt
    31981 ttaatttatt tcaatcaatg cgccattacg gatatggctg gcaaattctt gataagtttc
    32041 gtcaaataaa tcataatgat cacgcaagga cggatcaacc atttcttttg gaaagaaaaa
    32101 tcgctcatct ccttgttctt gtcccgctaa tgcagcaaca agctgactaa cctgtgataa
    32161 ttctatcaaa gttccgtcat ttcgcaaaat ctcgatttgt gtgcgatgtc tgccttgttc
    32221 tgggcgataa aaatcataag gtagatcgta gctagaattc acagcagtgt aataaacagg
    32281 attatatccg accttttcta ctaacaaagt gagttcttgg actaattttg aatcccggtt
    32341 gccagaaaaa gtagcagatt tcagtggttt acgattcaaa aaacgtttag ctaaatcatt
    32401 taaaatagga tcagcagatt cagaccattg agtaaagtaa gtgcccaaga ccccatcatc
    32461 aagtttgagg tattcttcta aagtaaagct tccgtcaaga aatggcagaa gtaattgaga
    32521 atggagttca aaactgctgg catcttcttg atacaactcg tgtgcgcgat gaagcagatg
    32581 ctccaaaatt acttccatac cacgggaaac aggatggaag tacacttgca catacatttg
    32641 atatcggcta acgatataat cttccactgc atgcatgcca ctcatggaaa aagcgatccc
    32701 gccttcataa ggacgaatca cacgtaatat tcgggtaaga tcaaatgttc catattctgt
    32761 tccagtgaaa taagcatccc gaagcaaata gtccattcga tctgcatcga tctgactaga
    32821 aatcatttgg acaacttgtg ggttggggta agttttttgg atcacactag ctaccttttc
    32881 aggaaaacct tcttctactc ggttaagaat ctgatacact tctgtttctg gagaagtgat
    32941 gatctctaca gtgatggctt catgattcgt atggaagata tgttcaaatg tatgagagta
    33001 aggaccatga ccgacatcat gcaacagtgc cgcacataaa gtcaccagcc gttcctgatc
    33061 atcccagcct ccgttgccta ttttttcttt tgaaaaattt cgagaaaaga tatcgcagat
    33121 tcgtcttgaa atctcataaa cgcctaatga atgagtaaaa cggctatgtt ctgctccgtg
    33181 aaaagtgaaa gaagaagtgc ctaattgttt aatgcgtcgt agacgttgga cttctttcga
    33241 attgatcaaa tctaagatta cttgatgctg gacatggata tagttatgga ctggatcacg
    33301 aaaaactttt tccataggca gcatttgata tttataaggg gttgtcataa tgtagctcct
    33361 ttatgttatt acgttgttcc attctctgga tttggttgtt ccattgctcc agctggattt
    33421 ctttttttag ccagtcggta gaatcctgta aatcacttgc agaatacagc ttttcaaaag
    33481 cttttgcaaa tgcttctttg acttctttta caaaaagggg ttggtcaagc agttcttcaa
    33541 gtgttgccat tgtagcagga tccactgcag gatagccatt tgtaccaaag tcttctttta
    33601 aagcagattg ataaaatcgc ttcatgactt gcccgcgttt ttcctgatta ccgttgacac
    33661 ttaagtacat cataacagaa atcccatttt tgatccgacg ctgagcgata ccggcaaatt
    33721 ttttgccatg gatgcttaaa tcataagttc caggacaata agaatgggtg atttcgaatg
    33781 cttgtatcgg tgcctttgga aaagcgtatt tagtcaatag gagcatttgc tcgtaagcgt
    33841 catctatggt tatttttttt gtgtcacttt gggggaaaat caaagaaata ttcaaaattc
    33901 cttcatctga tacgatccct aatccacctg aatttctgat cactggagcg taacctgcat
    33961 ctttcagtgt ttggatcccg tcacttagaa aaggagttcg aacatccttc atacctaaga
    34021 tgactgtgca gtcatattgc cagaaatgta aaaagatttg ctggtgttct ttagaatatt
    34081 ccgttaatat atctgttaat gcaaaagggg aaaagtcgtt tttttgataa atgtgctgat
    34141 ctagtcgaat cgcagggttc attcaaccgg ctcctttgta taaattcata ttttctacaa
    34201 tattatactc taaaatcaaa gagttgtttt ggaaaaaagg ttttctgtaa aaatggacaa
    34261 gttggttgac aaaacaaaag aaactaggaa caatgtagct atgagaagta aaggagaatg
    34321 aataatggaa ttatcacaag gaacaccgat ttcaatcaaa ttgcgtacaa aagtaaaaca
    34381 gcaaggggaa gtgcaagatt tttattttga tttgaaaggg caaatggtga caattggcga
    34441 tacgctgtac atacgttata aagagattca ggaggagacg ggcgatgaga ttcctgtaac
    34501 gatcaaatta gttccagatg gtcatgtaca gctgatccgt gcaggagaaa tgcggatgcg
    34561 tttgaaattt ggctataaag aacggttaga aacatcctat cgaacaccat acggtatgat
    34621 ccagattgct acttttacga aagaactgca tgttagtttg aaagaccgtc ccacaggagg
    34681 gaaggttcga atcgattatg atctgttcat gggaccggaa aaaatcggcg aatattattt
    34741 gaccttggat tttacagcgt aaatataaaa gaaaagtttg attttttgtg tctgattttg
    34801 tatgataagg agtagactgt gaaaggacgt gtcctgattg gaaattagtg tatttgaagg
    34861 tgcaaacaaa agcgaattat caatgatcga agtagcacac gcgatcttag aacaacgtgg
    34921 cgatgtcatg gatttttctg atttagctaa ccagatccaa aactatcttg ggaaatcaga
    34981 cagtgaaatt cgtgattcac tcgcacaatt ttatactgac ttgaatattg acggcagctt
    35041 tatctcatta ggggataatc gttggggctt gcgttcatgg tatgctatcg attcgatcga
    35101 tgaagaagta aaccatggaa tggacgaaga ggatgaagat actccacgtc gccgtaaacg
    35161 caagaaagtc aacgccttta tcaatgatga tgaagacgcg atcgattaca atgacgatga
    35221 cccagaagat acagatctga cagaagaaga tgacgatgat ctgtttgatg acgatgatga
    35281 tgaagatgaa gaaatcgctg cttataattc tgacttgcaa gaaatcggag cagatgaccc
    35341 gacagatgat gaagaagact tgccaggaaa tatcgaagaa gatttaagta tcatagacga
    35401 agacgatgat gatgaagatt atgaagagga agaattttct gactctgaaa aagaataaac
    35461 attattttct tacgagaggt tgtgacagag acgatcagcc tatcagaata aggaggtgtg
    35521 acacgat
//
LOCUS       DAZKBH010000016        35069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000016 DAZKBH010000000
VERSION     DAZKBH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.016"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..653
                     /locus_tag="ACG8OO_000805"
     CDS             201..653
                     /locus_tag="ACG8OO_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9044731.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(777..1628)
                     /locus_tag="ACG8OO_000806"
     CDS             complement(777..1628)
                     /locus_tag="ACG8OO_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9044732.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(1641..2426)
                     /locus_tag="ACG8OO_000807"
     CDS             complement(1641..2426)
                     /locus_tag="ACG8OO_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9044733.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(2778..3719)
                     /gene="ribF"
                     /locus_tag="ACG8OO_000808"
     CDS             complement(2778..3719)
                     /gene="ribF"
                     /locus_tag="ACG8OO_000808"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFP9044734.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(3723..4646)
                     /gene="truB"
                     /locus_tag="ACG8OO_000809"
     CDS             complement(3723..4646)
                     /gene="truB"
                     /locus_tag="ACG8OO_000809"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9044735.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(5014..7376)
                     /locus_tag="ACG8OO_000810"
                     /pseudo
     CDS             complement(5014..7376)
                     /locus_tag="ACG8OO_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            7541..7997
                     /locus_tag="ACG8OO_000811"
                     /pseudo
     CDS             7541..7997
                     /locus_tag="ACG8OO_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(8154..8501)
                     /gene="rbfA"
                     /locus_tag="ACG8OO_000812"
     CDS             complement(8154..8501)
                     /gene="rbfA"
                     /locus_tag="ACG8OO_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9044736.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(8528..10834)
                     /gene="infB"
                     /locus_tag="ACG8OO_000813"
     CDS             complement(8528..10834)
                     /gene="infB"
                     /locus_tag="ACG8OO_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9044737.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(10847..11158)
                     /locus_tag="ACG8OO_000814"
     CDS             complement(10847..11158)
                     /locus_tag="ACG8OO_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFP9044738.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(11155..11448)
                     /locus_tag="ACG8OO_000815"
     CDS             complement(11155..11448)
                     /locus_tag="ACG8OO_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFP9044739.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(11470..12645)
                     /gene="nusA"
                     /locus_tag="ACG8OO_000816"
     CDS             complement(11470..12645)
                     /gene="nusA"
                     /locus_tag="ACG8OO_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9044740.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(12668..13141)
                     /gene="rimP"
                     /locus_tag="ACG8OO_000817"
     CDS             complement(12668..13141)
                     /gene="rimP"
                     /locus_tag="ACG8OO_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9044741.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(13286..17644)
                     /locus_tag="ACG8OO_000818"
     CDS             complement(13286..17644)
                     /locus_tag="ACG8OO_000818"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFP9044742.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(17856..19565)
                     /locus_tag="ACG8OO_000819"
     CDS             complement(17856..19565)
                     /locus_tag="ACG8OO_000819"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9044743.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(19633..20901)
                     /gene="rseP"
                     /locus_tag="ACG8OO_000820"
     CDS             complement(19633..20901)
                     /gene="rseP"
                     /locus_tag="ACG8OO_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFP9044744.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(21062..21862)
                     /locus_tag="ACG8OO_000821"
     CDS             complement(21062..21862)
                     /locus_tag="ACG8OO_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9044745.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(21859..22671)
                     /locus_tag="ACG8OO_000822"
     CDS             complement(21859..22671)
                     /locus_tag="ACG8OO_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFP9044746.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(22866..23423)
                     /gene="frr"
                     /locus_tag="ACG8OO_000823"
     CDS             complement(22866..23423)
                     /gene="frr"
                     /locus_tag="ACG8OO_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9044747.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(23426..24148)
                     /gene="pyrH"
                     /locus_tag="ACG8OO_000824"
     CDS             complement(23426..24148)
                     /gene="pyrH"
                     /locus_tag="ACG8OO_000824"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9044748.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(24284..25165)
                     /gene="tsf"
                     /locus_tag="ACG8OO_000825"
     CDS             complement(24284..25165)
                     /gene="tsf"
                     /locus_tag="ACG8OO_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9044749.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(25264..26046)
                     /gene="rpsB"
                     /locus_tag="ACG8OO_000826"
     CDS             complement(25264..26046)
                     /gene="rpsB"
                     /locus_tag="ACG8OO_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9044750.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            26405..26884
                     /locus_tag="ACG8OO_000827"
     CDS             26405..26884
                     /locus_tag="ACG8OO_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9044751.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            27101..28192
                     /gene="phnW"
                     /locus_tag="ACG8OO_000828"
     CDS             27101..28192
                     /gene="phnW"
                     /locus_tag="ACG8OO_000828"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFP9044752.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            28185..28313
                     /locus_tag="ACG8OO_000829"
     CDS             28185..28313
                     /locus_tag="ACG8OO_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFP9044753.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            28317..28862
                     /locus_tag="ACG8OO_000830"
     CDS             28317..28862
                     /locus_tag="ACG8OO_000830"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFP9044754.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(29316..31007)
                     /gene="argS"
                     /locus_tag="ACG8OO_000831"
     CDS             complement(29316..31007)
                     /gene="argS"
                     /locus_tag="ACG8OO_000831"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9044755.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(31427..32374)
                     /gene="arcC"
                     /locus_tag="ACG8OO_000832"
     CDS             complement(31427..32374)
                     /gene="arcC"
                     /locus_tag="ACG8OO_000832"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9044756.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(32489..33508)
                     /gene="argF"
                     /locus_tag="ACG8OO_000833"
     CDS             complement(32489..33508)
                     /gene="argF"
                     /locus_tag="ACG8OO_000833"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9044757.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(33599..34828)
                     /gene="arcA"
                     /locus_tag="ACG8OO_000834"
     CDS             complement(33599..34828)
                     /gene="arcA"
                     /locus_tag="ACG8OO_000834"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFP9044758.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         9480 a         7505 c         6174 g        11910 t
ORIGIN      
        1 tcataatact gcccattatt ttcaggtata tttaaatata atatcacttt aggagagaac
       61 gaatttcttt agagaaataa aagttagaat tttctgactt gatttttaca atgaccttaa
      121 ttaaaatcgt tctttctaca tttcgtgatt aagcaaatat caactaagtc agtagaacca
      181 ttcacatagg agggtgaaca ttgaaagatt tttactcagt aaatgaatta gccgaacaat
      241 taggtgttac tacacgttct atcagaaatt atcttcatga aggaaaacta aaaggaacaa
      301 aggttggcgg gcaatggaaa ttttctgaac gtaatctttt tgagttccta tacggagatc
      361 aagcagagga agcagcaaaa gaaatgcagc gattcatgct aaatgcacca atcacgatgc
      421 gtttcaacct tcaatatcgt gattttacag caatcaatca attccgagaa caattagtcc
      481 agtaccataa cgatgtttac gcaaataaaa aagaccgtct tttacaatac gatctttata
      541 aagacaatca tgcggagata ctaatcggtg gtaacttcaa ttatgtcgta aactttagtc
      601 aatggatcaa tgaaaaatta ttgatccaga cagatatttc ccttgtatct taaacaatta
      661 aaaagcgcta gaaattccag agatgttgga aattctagcg ctattttttt ataagtactt
      721 actgtttggt tcaaacaaat atttttttaa taaatgaaat tttaccatct ttatcctcaa
      781 ataaagaccg tgaccatgct tcctactacg atcagcaata atccgataat cgttaatttt
      841 ttttcttttg gtgtcttatt ttcatgcagg atgtacatac cgcctaatgt agagataact
      901 acgtttagct gtgtaatgat atatgcagta gcaaccccat tttcatttgc tgaaaaaata
      961 taagccaatg ctgacacact aaagatggct ccaacgatcg ttgctttcca actcttgtct
     1021 tcagaaaatg cggttggttt acggaaaatc acataaatca ctgcccctaa aaagacaccg
     1081 atcatttgcg gaaagaaaat ggaaagtccg cttgcatcaa ctagtttagg caatgcactg
     1141 tatacccaat aaccaatact tgttgatgct aatagactaa ttccttttgt gataccgcct
     1201 ttttttccag gatcatcact gattgccgtt aaagcagaac caaatacaag aagaatgatt
     1261 gctgctgctc caatgatttt ccccatactt ccagaccatt ctccaaaagc gaagacacca
     1321 atcaacgagg ttccgactaa ctgtaatgca gtcgaaattg gcatcgtttt agaaacacca
     1381 atcaagttga aagatgtata ctgtcctgtt tgtccaataa cccaaaatgc accagaaagt
     1441 aaactaatca aaaatgtagt cactgaaata ccagctggag aagcgaataa ttgaacgagt
     1501 aagccgacaa gcaccgctcc aataccagtt cctaatatct catttgttgg tctgccacca
     1561 atctttttca aaatcagtgg ctggatcccc cagccaactg ccgggatcaa tgcaatcaat
     1621 aaacccattt ttattcaccc ttatcctttt ccaccttgga aagaaggata taaagtcatc
     1681 ccaccatctg caaatagtgt aatacctgta acataactgg attcgtctga agccaaccat
     1741 gcagctgtcg ccgctatttc ttttggttca cctacgcgtc ctagtgggat catactttcc
     1801 aagagttctt tttgttcagg atcatcaaat tttgccgcat taataggtgt acggatagca
     1861 ccagggccta tactattgat tctgattccc ttcggtgcat actccattgc taccgtttcg
     1921 gtaaataatt ttaccccgcc ttttgaagca gcgtaatgag caaacgttgg ccatgggatc
     1981 tgttcatgaa cagaagacat attgatgata tttccttttt tattattctc tgtaaagtaa
     2041 tttaaagccg ctttcgttcc gataaaaacg ccagtcaaat tgacttttaa cactctttcc
     2101 caatcttcaa ggctaagttc atgggttgga tgctgatttt ccatcccagc attattaacc
     2161 caaacgtcca gtgtcccaaa attttctaaa gccgtatcaa gtaaacgctg tattccttct
     2221 tcagaactga catctgcttg tacagagact gcttttccgc ctgcctcttc gattgctttg
     2281 actgcttttt ctgctccttc tttatccgag ttatagttga caactacatt cattttttct
     2341 ttcccgaatc gttgagcaat cgctgttcca atcccctttg agccaccagt tactactgcg
     2401 actttgtttt ctaattcctt gtacatagta cccactcctt tttcatacct aaacctgtta
     2461 ttacggataa cttttatttc cgccttaaag tgagtgtaca ccaagatata tagtctaaag
     2521 caaatgatac gctctagcaa tcgttttcat gaattgctag tgtcctttta tttctataaa
     2581 gaaagaggtt gtgacagaag tggtcagctt caagaaattt cggcttattt ccgaagaagc
     2641 tacttctgaa aaaccattta tttggaaaaa aaggctgtga caagactttt gtcacagccc
     2701 ctttcctggg cttatttatc acccatttta ttgtaattgc tagttataca taaacaagag
     2761 attttatgca taatgtttta ttgctgagaa aaatattcag ctgttgcttg ttcatcctcc
     2821 ttgagttgtt caatcaattc ctctacacta gaaaatttca cttgatctct taaaaattga
     2881 ttccagcgta ccgcgacatg ttcaccatag atatcttgat taaagtccaa aatatacaac
     2941 tctacagtca gctcccgtcc ttcaccgaat gtatcattat gtccaattga acccatggct
     3001 ggataccatg tttccccaat ttttatttcc gacacataaa caccttcttt aggcaaatga
     3061 accgtgctct ttactttgac atttgctgta gggaatccta gtagtctgcc tcgtgcatcg
     3121 ccatggacaa ctaccccttc tacctcatat gtgcaaccta atagttttgc tgcttctttt
     3181 acttgtccgc tgtctagtaa tccacgaata cgggtagagc tgattttttc accttgttct
     3241 tcttctttag aaactgtgac gattttgaat cttcctttgg catattcagg aaaatgagcg
     3301 acatctgcaa tgtcttttgg tccatacgta taatcaaaac cggaaacagc atattttgca
     3361 tgtaacccta cgatgtattg atcaacaaat tcctgcggtg ctaaatgagc aaaagaagaa
     3421 gtaaaatcaa cttcatataa ataatctata cccaaagcgg ccattttttc ttctttttgc
     3481 ttaaggctag tcaaatattt cacgctgcta ggatcgatct ttttaaagac aatagatgga
     3541 tgctgattga aagtcatcaa tgctaatttc aatccttctt tttgtgcaat ttccatacct
     3601 gtctttatga ctttctggtg tccgatatgc acgccgtcga aaaatcccag cactaaaacg
     3661 acatcgtctt ctggaatttg ttctgattgg taaggatgtc tgatcttgat gatattcatt
     3721 cgttattcct catttctcaa tactttacta ggttttaatt tgtttttttc tttcgggttt
     3781 ggctgataaa tactgacaac ttttccttta taaaacaaag caatttcttc tgacggcatt
     3841 tctgggagcg caaatacagt ataatcaagt ctcataccgt tttttacttt ctgccatact
     3901 tcatcagtaa tatctactcg tttgaaccgt tccactcctt tttctattgg cagcaaacaa
     3961 tcatcgattg tcccattttt cacagcttga gctacttctt ctaatgtcag agcatccttc
     4021 tgggacatac cggcacttgc tgttcgggtc agatcagaca tgtgcgcagg gtaaccaagt
     4081 ttcttgcctg tatctacagc taatgtgcgg acataggttc ctttcccgca ttctacttcg
     4141 aagcgccaag aaatggttcc tgtcacttcg tctcttgtca attcacttgt ccggataaaa
     4201 cggtaaatct gagcctttct aacaggacgc tccactgttt cattattgcg ggcatattca
     4261 tataaccgct taccgttgac ttttaccgct gagtacattg gtggtatctg ggtgatctca
     4321 ccaataaatg aagccatcac ttcatctatt tttttttcag ataaagacat atctactggt
     4381 tttgtttcta cccgttctcc ggatcgatct tccgtcgttg tactgaaacc tagagttatc
     4441 tcgcctacat agattttccc agaatctgtc aaatattcga taacttttgt ggcttttcca
     4501 atacaaatgg gcaacacacc gtctacgtct gggtcaagcg tacccccatg tccgattttt
     4561 ttcgtatgta agatttttct taatttgaat acgcaatcat gacttgtcat tccgcgttct
     4621 ttccataaag gtaataaccc gtccatgtct tttctccatt tcatttttga tttaactgaa
     4681 ttattataac acattgcatc aattactgac gaatttgaca tcataatgaa cggtttttat
     4741 ttttcggcaa taaaaacgta acaaaattca tgctcacgta aatagaaatc atgttatgct
     4801 tttttaaagg agggaataga atggaagatt ttttctttgc accatttgat tggatttatg
     4861 ggtggcttac aggtaaatag tttggttttc acttcccaaa aacgaaaaaa aagactggcc
     4921 attcttccag tcttttttcc gtttttttta tttatttttt cagtttctta atcaatgtaa
     4981 aaaattctat tttgcgggat cgattttatc attttattgg tcaagatttt tccatttcca
     5041 atccaatact tctggcagat caataccatt ttcacgaata tagttttgat gttcttgtaa
     5101 tttctgatcc atttttttcg caaaatcttc tgcttgactt cctttgattt ttaaagctgc
     5161 gtcttttgcc aaatgataac gatcaagttc atttactaca cgcatgtcaa atggtgtcgt
     5221 gatatctcca ttttctttgt agccatgaat cattaaatga tggttatgac gatcaaagaa
     5281 aatcgtgcgg accaatgttt cataaccgtg gaaagcaaat aagatcggtt tgtcttttgt
     5341 aaaatattga tcaaattctt catcgctcaa cccacgcgga tcttgacttg gatgacgtaa
     5401 tttcaataaa tctacgacat tgatgaaccg aatttttagt tttgggaatt cttccagcag
     5461 caaagttaca gctgctaatg cttctaagtt agattccgtt cctgcagcag caatgaccaa
     5521 ttcaggttcc tcctcttttg ctgtcgaagc ccaatcaatg attttcaagc cttcattcac
     5581 taagacggct gcttcttctg ctgagtaaaa ttgagcacgc ggatgtttgg aagagatgat
     5641 caaattgatt ttttctgacg agcggaatgc tttatccatg actgcgagca atgtatttgc
     5701 atccgcaggc agatattcac ggatgaactc cgccttcttt tctgccaaat gtgtcaatat
     5761 tcctggatct tggtggctat atccattgtg atcttgctgg aagactgttg agcttgcaat
     5821 gagatttagc gaaggataat catttcgcca agaaaggtca tgtgatttcc gcatccattt
     5881 gaagtgctga gtcaacatag agtcaaccac tcgcaagaat gattcgtaac ttgcaaaaaa
     5941 ccatgccgtc cagtcagaac gtacccttct aagaaccctt catcttggtg ttccgataat
     6001 tgactgtcga tgacgcgacc agatggcgaa agccattcat cattttctgg ttcgattttt
     6061 tccatccatt ggcgatttgt cgttttgaaa acttgattca atcggtttga ttttgtttca
     6121 tctggtccaa atatacggaa attttctgga tttttctcaa tgatctcgcg aacgaattta
     6181 ccaagttcgg tcatatcttc tgctttaacg gagcctggtt tttcaaattg caaagtatat
     6241 tttttccaat ctggcataat aagcggtctt ggatcgattc caccattggt gataggattt
     6301 tttgccattc tttggtcacc agtaggcgcg atttctctga tttcttctaa aatacgtcct
     6361 tgtgcatcaa ataatttttc tggttcatag gatttcagcc aacggagtaa agcatctgca
     6421 tgatccatat gttcttgatc tacaggaatc ggtacttggt gtgctcggaa ggttccttca
     6481 attggttccc cgtcccattc ttttggaccg gtccatcctt ttggtgtgcg tacgatcaat
     6541 actggccatt ttggcatcac gacatctgtt gctgtcttca ggcgagcttc tttttggatg
     6601 gatttaattt tctcgattgc ttgatccatt ttttcagcca taacaggatt taatttttct
     6661 gaatcatttc cttcaataaa gaaaggttcc cagccaagtc cgttaaagta atttgctaat
     6721 tctccatctg atttacgtgc caagatcgtt gggttggcta tttttgcacc atttaaatga
     6781 aggatcggta aaacagctcc atctatcact ggattgataa agacatttga gaaccagctt
     6841 ccagctaaag gaccggtttc tgcttcacca tcaccaatca cgacagcaga gattacttca
     6901 ggattgtcta atacagctcc gactgcatgt gaaagagagt atccaagctc tccgccttca
     6961 tggatagaac ctggtgtttc tggtgctgca tgagaagcaa tgcctccagg aaatgaaaat
     7021 cgtttgaata atttttgcat tcctgtctca tcttccgtta cttctggata gatctctgtg
     7081 taacttccat ctaaataagc attcgatacc atgacttgtc cgccgtgtcc tggcccttca
     7141 atataaaaca tgttcaaatc atatttattg atgactcggt tcaaatggac ataaataaaa
     7201 ttttggccag gaatcgttcc ccaatgaccg attggatgtt ttttgacatc ttcgggtttc
     7261 agtgtacgtc tcagtaaggg gttatcttta agataaagtt gcccaacaga aagataatta
     7321 gcagcacgcc accaagccgt cattttatca aagtattctt tactagaata atccatacat
     7381 aacactcctt ttcattatta ctcttattct agcgcagata ctcccaattg aaacctaata
     7441 agcacttctg ttcatttgtt tggattttat atctagccaa atgaacaggt ataaaaaacc
     7501 acgaaatttt tcctttcgct gacgtttttt acattgatcc aaaaaacttg tcgttatcac
     7561 aatggctgtt ccgcagttca tttcgctcct catcatgcat aatttgctga atgacgctgg
     7621 gccagttaac acattgctgg aaaactgggg ctggatcgag caattccttt cctgattgat
     7681 ccgttttatg taaaagtctc tgttatcttt gtcaatatgt gaattggtat cccagctact
     7741 atgctaattt ctacagggat tatccaaaat ttggatcgtg accaaatcga agcggcgtaa
     7801 tcggaaatat caataacttc aacgtgattt ttcttttaac tgtcaacgga ccgacaaact
     7861 caaactttta tggtgcaggt tccactgatc ttctagttac ttggctatac ctgactgtca
     7921 ctaaaataga ttacaatcta gcttccgtta tcggtatctt gatctttatc tatatgcaaa
     7981 aatttatata ctactagaaa aaagaaagtc gaatttttta cttttctttt tctctttttt
     8041 ggttagctga cacgccgcaa gactaaagtc atggaactct taggtaatac gtcttaatga
     8101 aaacaaaaaa atcccgcaac ccctgatttc acaggatttg cgggagtttt tgattagtct
     8161 ttgttcaagt cgcgaagcaa ttcatcgata tggttgccat agacaactga ttcatcaagt
     8221 tcaaagaaca attcaggtgt cttataaata cttagattgt gccctaactc tcgacgaatc
     8281 aatcctgatg ctttattcaa tccatcttgt gctgttttct tatctgatgc taagtcagat
     8341 aaaatgctgt aatagatcgt tgcttgctgt aagtcccccg taactttcac atctgtgatc
     8401 gttacgttct ccacacgagg gtcacggacc tttttgcgca aaatatcatt gacttctttt
     8461 aagatttctt gaccgactcg gcggtcacgg tagttagcca taatgattgg cctcctttta
     8521 tcagttttta gtcatttttg acttcttcca tgataaatcc ttcaattacg tcatctactt
     8581 tgatgtcatt gaatttttca atcattgcac cacattcata acccattttg acttctttca
     8641 cgtcatcttt gaatcgtttc agacttgcaa gttggccttc atagattacg attccgtcac
     8701 ggattacacg gacaccgcta tctctacgaa tatatccttc tgttacatag gcacctgcaa
     8761 tcgtaccaac tttagaaact ttgaaggttt cacgaacgat catttgaccg gtgatttttt
     8821 cttcaaattc agggtctagc atccctttca tcgcagtttc gatttcttcg atggctttat
     8881 agatgatacg gtgaagacga atatcgactt cttcagtatc agcttgcact tttgcctgcg
     8941 gtgtaggacg aacgttgaaa ccgatgataa tagcgttgct ggctgctgct aaggttacat
     9001 cactttcatt gattgctccg acagctgaat ggacgatttt tacacggaca ccttccacat
     9061 caatcttctt caatgatgca gccagtgctt ctgctgaacc ttgtacatct gctttgatga
     9121 ttacgttaac ttctttcaat tcgccttctt tcaggctttc aaataagtta tccaaagtca
     9181 cacggcttgt ggcagcacgg ttttctaata atgcacgttt cgcgcgttct tcacccgctg
     9241 cacgtgctgt cttttcgtct tcgaagacta cgaaacggtc acctgcttga ggtacatcat
     9301 tcaagccagt gatttctact ggcgttgcag gtcctgcttc tttatcgcga cggccaagat
     9361 cgtttgtcat tacacggaca cgtccgtaag tattaccgac aacgattggg tcaccaacat
     9421 gaagtgttcc ttcttggaca agtaaggttg acacaggacc tttaccttta tcaagacgtg
     9481 cttcgatcac tgttccgatt gcgcgttgtg ttggatctgc ttttaaatct tctacttctg
     9541 cgactaacag gatcatttcc agcaattctt cgatgttttg gccgaatttc gctgagatct
     9601 caacaaagat cgtttctcca ccccatgctt caggaattaa ctcatactca cttaattctt
     9661 gcataacatg ttgagggttt gcacctggtt tatcaatttt atttacagcg acgatgattg
     9721 gaacgcctgc agctttggca tggttgattg cttcaactgt ttgtggcatg acgccatcat
     9781 ctgcagcaac aactaagatc gtgatatctg tgatacttgc tccacgtgca cgcatacttg
     9841 taaaggccgc atgtcctggt gtatccaaga aagtgattgg tttaccatca atatcgatct
     9901 ggtaggcacc gatatgctgt gtgattccgc cggcttcgcc gcttgttaca cgtgaatggc
     9961 gtaatgtatc caataaagtt gttttcccat ggtctacgtg tcccataatc gttacgactg
    10021 gcggacgtga gactaattta tctggatcga cttcttctgc ctcgaagaat ttatcgatat
    10081 cagcaatgtc aacttggact ttttcttgtg gttccatgcc atagtccgtc gctaacagtt
    10141 caatcgtatc tttatccagt gcttggtttt gattgaccat gacaccaagc ataaataatt
    10201 ttttaatgat ttctgctggc tcacgatgaa tttttttcgc aatatctgct acgttcatac
    10261 cttcagtata ctctaataca tctggcaatt cacggaattt gcgtggtggt acagctggtt
    10321 tagttgattg ttgctgtttg ccttttttac cttttttatt ataacgatta cggttattat
    10381 tattaaattt gccgcgattg ttattgtttg gtttgttgtt attgttagta cggttttgat
    10441 tgccgccttg gctcgttcct tgacgatttt ggctgctggt atttcccgta ttattttggt
    10501 ttgttgttgg tttttttcct tgattcactt gaccgctccc ttgaccgcga tcttgataac
    10561 tgcggttatt tttatttttt tgttgattgt tcgttgtttc ttttttacgt tgatcgttcg
    10621 gctgttgatt ggctggtttt ctgcttgctt gttgtgcagc ttgttgtttc gtgcttgggt
    10681 ttttgaacgc ttgttgcaat ttcttctcat cattgctagt aatcgcaccc atatggtttt
    10741 tcacatcgat gccaagttgt tgtgcttttt ccacgacgtc tttactagat tttttgagtt
    10801 cttttgctaa ttcatagatt cttttgttgc ccatgcaatc accttcctaa ccttcaatta
    10861 gctccttggt cttcttcgca aatccggcat ccattattcc gaataccttg cgaggttttc
    10921 cgatcatctg cgtgatttct gcttcagaaa atagctcaaa gcaaggaact tcataatatg
    10981 aactcttatc ttttatcttt tttcttgtat tctcgcttgc atcggttgcc acaaacacca
    11041 attttgcttt ttgtttgcgg atttcagcaa tggtcagttc ttcacccgtg attagttttc
    11101 ccgcgcgcat cgctagaccg atgagattca tggctttttg ccgattaact gtattcattt
    11161 tccaaataac tctttacgtg ctttttggtg agtgacatag tcaagaagtt cttgataaaa
    11221 ctcatctgtt aatgttgttt cgagtactcg atcgagaatt tttttatccc aagcttgttg
    11281 tacttcttcc ggctcaagtg agacatacgc tcctcgtcca ggcatttttc cagttggatc
    11341 gatcgatact tccccttctt ttgaacgggt gattcggacc atctcttttt taggtttcat
    11401 ttcacctgaa accacggatt tgcgtaaagg aattttcctt tgtttcatct ctttgcctcc
    11461 ttactaaagt taagcttctg gtttttcttc gaccgtttca ttgttgaatg caattgtttc
    11521 atattcatcg tctgttaaat ctgactgaac gattgcttca tcatgcagtt cttctgtttc
    11581 tttttgttgt tcttgatttt cataaaattc cgtcatgtca gattctgatt tgatatcgat
    11641 tttatggtta gtcaatttag cagctaaacg tgcgttttgt ccgcgtttac cgattgctag
    11701 cgataattgg tagtctggaa cgacaactgt gcaggccttt ggatttgtct catcgaagat
    11761 cacatccatg acttgtgcag gattcaatgc atttgcaatg aatacagctg gatcttcatt
    11821 ccattcgacg atatccatgt tttcgccttt taattcattg acgattgctt gaactcgttg
    11881 tccctttggc ccgacacatg taccgactgc atcgatattt ggatcatttg agcgaactgc
    11941 cacttttgaa cgatcgccag cttcacgggc gatgctgaca atttcaacga ttccatcata
    12001 gacttctggt acttcttgct caaataagcg gcgcaatagg tctggatggc tgcggctaac
    12061 aaatacttgc ggacctttag acgtattttc tactcgggaa acataaactt tgattcgatc
    12121 atgtggttga taaaattcat ttggcatttg atcttgttta gaaagaactg cctcaatttt
    12181 accaagattg acataaatat aacgtttatc ctggcgttcg acgattcctt gcatgatgtc
    12241 tttttcataa gcactgaatt cgttataaat aatcgttctt tctgcttcac gtacacgctg
    12301 taaaataact tgtttagccg tttgcgcagc gatccgccca aaatcttttg gtgttacttc
    12361 aaaacggatc gtatcaccga tctcatatgc tggattgatt aaaagtgcat ctttcaatga
    12421 gacttccaac tgtgaatcca tcacttcttc cgttacttct tttaccgcat agacatggat
    12481 ctttcctttt ttctgatcaa attccacttc gacgttttgt gcttggccat agtgacgttt
    12541 gtatgcagaa accaatgctg cttctaaagc atcgatcacg atttcttttg agatcccttt
    12601 ttcagcttct aaagcgtcta acgcatttaa catttcttta ctcatttttt ctgattcctc
    12661 tccgtgatta aaattggata gctaagcgag cttttgcaat attttttcgg tcaaaagtca
    12721 attctttttc tcgtgttttt atgcgtattt tcaatgtaag ctgttcagca tcaaatgatt
    12781 gaagaaatcc ttcatattgt ttttcgccat ctactggttg gtataaggag acatggacat
    12841 aatcaccaag tgccttttca tagtcgcttt cttttttcaa tggccgctct gctcccgggg
    12901 aggatacctc caggaaataa gcttgaggaa tcggatctgg atctgctgca tcaagttttt
    12961 cactcagttt ctcactgaca aaggcgcatt cttcgatatc gataccgcct tctttgtcaa
    13021 taaatactcg taagaaccag ctttttcctt cttttacaaa ttcgacttcg acaagttcaa
    13081 aattttgttc atctaaaatc ggtgtcacca tttctgtgac tgtttcaacg acgctactca
    13141 aaagtttcgc ctccttaaaa aattaagtgc gtaattccat taaaaagagt gagcgaaaaa
    13201 tccgctcact cacagttagt atcattacct ataagaacta taccatactc tgcttaaaat
    13261 tacaagcaga caggaaaaca gatgcttata gcatatcgaa taacgataat tgattttcat
    13321 ctggtagatc tttcaatacg ccattgtcgt tcatatattc gatcaatgtt tttgaaactt
    13381 taccgcgggt ggcaagatct tctttggata aaaatggacc gtcttttctt gcttcaacga
    13441 tttgttttgc cacgttggtt cccaaacttg ggacagctct aaatggtgcg atcaacgtat
    13501 caccttcaat aacaaagtta acggcatccg atttatacaa atcaatcatt ccaaatttga
    13561 atcctcgttc cagcatttca ttggctaact ccagcactgt cagttgattc ttttctttta
    13621 cggaagcatc taatcctttg tccgtgattt ctttcattgc ttgtttcaca gcatcttttc
    13681 ctttgcacat ggagactaga tcaaagtcgt ctgcacgtac agaaaagtat gcacagtaat
    13741 acaaaatagg gaaataaacc ttgaaataag cgacacgaag agccatcaat acgtaagctg
    13801 cagcatgggc tttcggaaac atatatttga tttttgaaca agaatcgata taccagtcgg
    13861 gcacattatt ttctttcatc gccgatagat aggtttctcg caattcatca gggatcttgt
    13921 tccattgtcc tttacgaaca gtttccatga ttttaaatgc catcccgcta tctaatccag
    13981 catggatcaa ataaaccata atatcatcac gacagccgat tacttccgcc agtgtcgcat
    14041 ctcctcgtcg aatcaattct tccgcatttc ctaaccatac gtctgtacca tgagatagtc
    14101 cggaaatttg cagtagttct gcaaaagttg taggatgcgt ttcttctagc atccctcgaa
    14161 caaaacgagt gccgaattct ggaataccta atgtccctgt tttggaataa atttgttcct
    14221 gacttactcc aagtacttca ggaccggcaa atatccgcat cacttccggg tcatccgtag
    14281 ggatcgtttg tggatcgatc cctgataaat cttgaagcat ccgaataaca gttgggtcat
    14341 catgccctag aatatctaat ttcaatacat tatcgtgaat cgagtgaaaa tcaaaatgag
    14401 tcgttttcca ttccgaattt tgatcgtctg ctggatattg gatcggcgta aaatcataga
    14461 catccatata atcgggaata acgatgatcc cccctggatg ctggccggtt gtccgtttga
    14521 ctccagtagc gccttttgct aatctgtcga cttctgcact tcgaaactgc agattattgt
    14581 ccctttcgta tccttttaca tagccatatg ctgttttgtc ggcgactgta ccaatcgttc
    14641 cggcacggta aacatagtct tcgccaaaca atactttcgt atagttatgg gcttctgctt
    14701 gataatcacc tgagaagttc aaatcaatat cgggcacttt gtctccatgg aatcctaaaa
    14761 atgtctcaaa tggaatatca tgaccgtctt tgtttaagcg tgcaccacat tttggacatt
    14821 gtttttctgg catgtcaaat ccagaaccat acgtaccatc ttcaaaaaac tcggaatatt
    14881 ggcactctgg acaataataa tgaggggcta acggattaac ttcagtgatt cctgtcatcg
    14941 tcgcaacaaa gctagagcca acagagccac gtgaacctac tagatagcca tcttcattac
    15001 ttttatgcac tagtttttgg gcgatcagat aaataaccga gaagccatta ccattaatag
    15061 aattcaactc tttttttaat cgtttttgaa tgatctcagg taacggatct ccgtacattt
    15121 gttttgcttt tgtgtaactc aattctgata tttcatcttc tgaacctgga atcttcggtg
    15181 tataaagttc atctttgact ggaatcactt cttcgcagat atctgcgatt ttattcgtat
    15241 tttctactac gatctctttc gctgtttctt cccctaaaaa gtgaaatgca gttagcatct
    15301 catcggttgt tcggaaatgg acatccggca aactatgacg attcaacgga ttcgctccgc
    15361 ccatagaatt gatcaaaatt tttcggtaaa tggcatcttc ttcgttcaga taatgtacat
    15421 tacctgttgc aacaacgatt ttatccaaac tttttccgat ttctaccaaa ttttggataa
    15481 tttcttccaa atcatgttca ttttttacca attcttgctc gatcaaaggc gcatagaccg
    15541 cttttggcat aacttcaata taatcgtaga attttgctcg gtttcttgcc tcttctaccc
    15601 ctttttgcat catggcttcg aatatttctc ccttatcaca agctgagccg atcaatagat
    15661 tttcacgcat ctttttcaat tgggagcgtg gtattcgtgg gacgcgttcg aagtattcga
    15721 cattcgacat tgaaatcagt ttgaacaaat ctttcagtcc tgcctgattt tttgctaaaa
    15781 tcgttacatg gaaaggtcga gcccttttgt aagaatctcc ttctccgata tgctcattta
    15841 gctgatcatg ataaagcatg ttatgattgt ccattgcctc tttcacaaag atccatgcta
    15901 agtgaccagt tgcttcagca tcatagattg ctcggtgatg ctgttccaag ctgacaccaa
    15961 attttttaga taacacacct aatccgaaac gtttgaattg aggatacaga tatctagcta
    16021 attccaacgt atcaatgact ggattggccg cttcaggaat accatacctt gcgtagctag
    16081 tattcaagaa ccccatatca aaagccgcgt tgtgggctac caaaattgta tcttttgaaa
    16141 attcaaggaa catccgcaat acttcttctt ccgactttga tcctcgaacc attccatccg
    16201 tgataccagt cagatctacc gttgttcgtg acaaaggatg tcctggatcg ataaattcat
    16261 caaagctttc gatcacattt cctttataca ttttgactgc ggctagctcg atgatcgtgt
    16321 catatactgc agacaatcct gtcgtttcca cgtcaaatac gacatatgtg gcttcactca
    16381 atgcttcatg tgcatcatta taagcaattg ggacaccatc atctacgaca ttcgcttcca
    16441 cgccgtataa gattttcaca cctgcttttt ttccagcact atgtgcttcc ggaaaggctt
    16501 gcgcaccacc gtggtctgta atggcgatag ctcggtgtcc ccatttccct gcttgtgcca
    16561 caagatcaga gatgctgttt gttgcatcca tcgtactcat attgctatga acatgaagct
    16621 ctactcgttt ttccccttca ggagcataat ctttgcgagg tgtatgtttg acttctatga
    16681 tatcttgcgc attcatgacc aaatcacgga caaacgtatc ttcttgaata cttcctcgta
    16741 cctttaacca gctaccgaca ctgatcgcat caaatacttg ttcatctttt tcgccgttcg
    16801 agaatttttt gacgataaat gatgaagtat aatctgtgat tttcaaagtc aagatcttgc
    16861 gttttgaacg tagttctctg acttctttgt cgaatacgta cccctcaagt gtgactcttc
    16921 gttcttcttc aacgatactg atcattggtg tcgttggttc atcggctgga atatttcgtc
    16981 ctagctggat gtggatgggt ccttcaagag ctggcgattg ctcttttctt tcctttttct
    17041 tttgttcgtg gacaactaga ctttcagctg cttgcttcat aaaggcttct gcctgttctt
    17101 gtttgcgttc ttcaaaaagt ttcaatactc gttccgcttg ctgctcatcc acttctggtt
    17161 cgatacgaaa ttttgggaat ccgtaactga cgaacaactc ttctactaat ggcaagtatt
    17221 gttgcttcaa ataagaaata gctcctgtac tgtcgattgg caagatgact ttacgatctt
    17281 ttatcacagg tacctgcgtt ttcaatactt gctgtgctag tggtgtatcg cattgctgat
    17341 tttccaaagc ttgggcccag taatcttgca agagctgttc atcgaatgtc tggtcatctg
    17401 ctaagatatt cagtttgact tgtgcaatat ctttaaaagc gatagtcaga tgctgagtca
    17461 atgaccgata tagcataata ggcaaaattt tggaaaaatg cagcgtaaat tcccataaac
    17521 gcgatttacg atggaccact acactatcca tttcaccagc agtaagcagt ggatgatttt
    17581 tttctgcttc ctctagttgg atttgttcca acagtttttc aaataaatct cgtttttctg
    17641 acaaagatat acgccctttc tgatgagaaa caatgcaatt gatcgcatct tatcctcttc
    17701 tttttcagta ccttttagta tactcgtttt ttctgagttt tacattaaaa aattggaact
    17761 gcgattcaaa tcgcagttcc aattttaaaa gcgaatcatc agattgtcaa aaacagctga
    17821 ttcagctatt tttattttct gtgttttaac gcttattatt cgccttggtt caagaggatt
    17881 ggtaaagtat tcaccaattc ttctttacgt acttcaacca tttcgccagt tttcttgatt
    17941 ttcacttcta cgattccatc cactgctttt ttaccgacag tgattctgat tggacagcca
    18001 atcaaatcag aatccgcgaa tttcactcct gcgcgttcat ttctgtcatc aaccagtact
    18061 tcatagccag cttttgtcat cgcttcttct agttcattcg tcaggtttgt ttggtattcg
    18121 tcttttagat tcatttggac gatatgcaaa tcaaatggcg caattccttt tggccagttg
    18181 attccttgtt catctgaatg ttgttctacg attgctgata ataaacgact tacaccgata
    18241 ccataacagc ccataatcac aaatttttct cgtccatttt cgtctagtac agttgctccc
    18301 atagattcac tgtaacgagt gccgattttg aagatatgtc cgatttcgat tcctcgagta
    18361 aaggcaagaa caccattgtt gtctggtgaa ggatctcctt cttgtacaaa acgaagatct
    18421 tcataagaaa gtacagtgaa atcgcgttct ggattcacat ttttatagtg gaatcctgtt
    18481 tcgttcgctc cagtaatggc attcgctaaa tcttggacat aacggtctgc atatactttt
    18541 acgtcatctg aaacaccgac tggtccgaca gaaccaaact cagcacctag atatttttgt
    18601 gcttcttctt ctgttgcttc ttctaaaaag tcagcagata aaaagttttt cagttttacg
    18661 tcattgactt catggtctcc gcgtaccaaa acaaggacag gttgttcatc cgcaatgaac
    18721 aatacagact tgatgatttt ttgaggttca acagaaaaga aatctgctac ttcgtcaatt
    18781 gattttacgt taggtgtagc tactttctcc aattcaagct gtgtttcatg cgattttttt
    18841 gatacatagt agcttgtagc catctccaag tttgcagcat aatcactttc agtcgaataa
    18901 cagatcgtat cctccccgat ttctgagatc gccatgaatt cttttgagtc tttcccgccc
    18961 attgcgccac cgtctccgat aatcgagcga aattctaagc cacaacgctt gaatacttcg
    19021 gtgtaggctt tttcgtaatc tttatatgtc tcgtccaaac tttcttccgt tgcatggaaa
    19081 gaatacgcat ctttcatgat aaactctctt ccgcgaagca aaccgaagcg aggtcgtttt
    19141 tcgtcacgat atttaggttg gatttgataa agatttaaag gtaatttctt atatgaattg
    19201 atttcattac gcatcaaatc agtaaaagtt tcttcgtgag ttggtccaag gatcattttt
    19261 cgatcattgc ggtctttgaa acgatacaag tttggtccat atgtttcgta acgcccggat
    19321 tcttcccaaa gttctgctgg caagatggca ggcatcagca tttctaccgc tccgattttt
    19381 tcaaattctt cccgcataat gcgtttcagt ttttccaata cacgatttgc taatggcaga
    19441 tatgaataaa tacccgctga tacttggcgg atatatccag ctcttagcaa gatttgatgg
    19501 cttaatactt ctgcatcatt tggaacttcg cgtaatgttg gaatcaacat ttttgattgt
    19561 ctcattctat atactccttt atttcctcaa aatagttatc gtttgttatt ataccctatt
    19621 tgtcaacttt ttctagaaaa agaatcgttg aatatcgttc catgtaacta gcaccatcaa
    19681 caacattaaa aatccaaagc cagctaatgt caatattcct tctttttctt gacttaatgg
    19741 tttcccgcga atcccttcaa aaatattcaa gactaatttc ccgccatcta aggcaggaat
    19801 cggaagcaga ttgacgattc cgagattcat tgaaagaagc gccatcaaac taataacagt
    19861 tgttacacct tgatttgctg cttctgaaga caattggtac atcatgacag gtccgcctaa
    19921 tttatctaaa ctgaaacctg taaatagtga tcctaaggct ttgaaaattt ccaatgaacc
    19981 gctgaaagct tgacgcgtcc ctccaatgat cttatccata aaacctgtat tcattggggc
    20041 ttttatacca agttggccaa ctttttcgcc atttgactct acagatttcg gtgtcacgtc
    20101 gacagattgc acctgacctt ctcgttcgat cttaaagtct aatgttttat ccggattttt
    20161 cgtaatgact gttgtcagat cattccatga atggatttct tttccgtcca cactgactac
    20221 ctcatcattt tcttttaagc ctgcttctgc cgcagctcca ttaggcatga tctctcctac
    20281 gcgattagtg ttcgtcactt gaacaccacc ttgcataaat gccagaatga tgaataacag
    20341 gattgccaaa ataaagttat tcatgggtcc agcaaagttg gtcagcatac gctgccacaa
    20401 tttagccgat tggaactgta cgtcttttgg cgcaattctg atttctgttc cgtcgttttc
    20461 aataatcgtc gcatcgtgat caacagggta ggttactact tctgtttcgt ctccattcac
    20521 ataaccagtg atagtcagct catcttctaa atcataacgg ctcaattcca tcgggatagc
    20581 atttgtcaat tggattttct tacttaaatt tatcttctct acgatgccgt cagaatttag
    20641 taaaagagaa agaggcattc ctggtgccat ttctgtttca tcatccccat tgcctgccat
    20701 tcgaacataa ccgccaatag gtaataggcg aagcgtatat gttgtcccgt cttttgcttg
    20761 atgcccataa attttcggac ccataccgat agcaaattcg cgaacgagga ttcctgatcg
    20821 tttcgcaaag aagaaatgac caaactcatg aacaatcact aatataccaa aaacgatgat
    20881 aaatgtcaga atcgttttca tagctttttt ctcctttact tgttttttgt gttttttata
    20941 aaacaacgat aaaacactat aattttacca tagtatacca aagacaccaa tagtatcaat
    21001 ataatgaaaa ataaaaacct gcgtctttta tgaaattcac aaagaaacgc aggtatactt
    21061 actaaataat gccaaataaa tgcatgatag gaaatacaaa cagcaaactg tcgaaacggt
    21121 ctaagattcc accatggcct ggtaagatat ttcccgagtc tttcacatca taatgacgtt
    21181 tgattgcaga ttcgaccaaa tcaccaaatt gtccgactgc tgaaaaaata atcgtcatca
    21241 gaagcatgac aaacatacca tgattaaata gttcttttgc tgggtatagc aataaataaa
    21301 gaaatgcgac tacaacagca ctaccaatac caccgagcgc accttcaatc gttttgttgg
    21361 gcgaaatatc aggacttaac ttatgctgac cataacgtcg accaaccata tatgctccga
    21421 tatcagttgc ccaaacaata aatagaccaa atatcaaaac agccaagctt tctgttcgtg
    21481 cattgacaaa attctggaaa cctattccca catacaagct gacaagcaca ggaaaacctg
    21541 cttcatcaat cgtatacgta tttttagaaa taactgatgt tcctaaaaga atcataacta
    21601 taagataaaa taacataaat gtacttacat tctctggaag aaagaaaaac catctttctt
    21661 ttggcaaaac aagtaacact gcaccaacgg cggataaaac tccttcaaaa cttaagagtg
    21721 tcaatccttt catccgaaac agctcgtaaa ctcctattac tgctaagatt gctgctgtaa
    21781 gctcaaccgc tatgcctccg atccatataa tcggaataaa caagactagc gccacggctg
    21841 ctgtgaccac acgttgtttc atgactgatc ccctttctca tcttctgttt tttttacacc
    21901 gccgaatcga cggtctcgat tttgataaga ggcgattgct tcttcaagat gcgcaccatc
    21961 aaaatcaggc caaagcgctt ttgtaaaata cagctcacta tatgcgatct gccataacag
    22021 aaaattacta attcgctctt ctccacttgt acggatcatc agctcaggat cttgtaactc
    22081 tttaggcaag aaacctgtca ttaaatgctt agcaatcaat tcatcatcga tttcttctgc
    22141 gtgtatttct ccttttgcta cttcttcggc aatttcttgg acagcagtca cgatctctgc
    22201 tctgcttcca taattcaaag caaaattcaa taccattcca gtattgtttt ttgtttgctc
    22261 aattgcacgt cttacagcat cttgcgtatg ttctggcaat acgttttcat aacccatgac
    22321 atgaactttg acattttctt taatcaattc tggtacgaac gtatcaaaga agtcgacagg
    22381 tagttgcatg agaaaactta cttcatcttt tggtcgcttc cagttttcag tcgaaaaggc
    22441 ataaagcgtc aacactttca ctccaagcct tgatgctttt tttgtgactt tctttactgt
    22501 ttccatgcct tctttatggc cagcaactcg tggcaaacga cggttttgcg cccaacgacc
    22561 attgccgtcc atgatgatgg caatgtgctg aggaattggt ccatcggtat taaattgaaa
    22621 ttcgcttttt tcttgaatat atttgttttt ttgcggaaaa aaacgtaaca ttctttttcc
    22681 tcctatactt aaaagattcc cactatctta agtattatag caatatctaa ctgtttttcc
    22741 tatgaaatca ttgttttttt atttaaaaac tgaataattt tttctttttt taatttttaa
    22801 ataaaagaaa tataaataaa aacagaggaa gcggaatgat ccgcccctct gtttttatga
    22861 ttttattaaa cttccaataa ttctttttct ttatcagcag cgatcttatc tacttctttg
    22921 atgctgttat ctgtcaatgt ttgtacgtct ttttctaatc cgcgcaaatc atcttcggta
    22981 atatcaccat ttttttgttg ttttttatat tcatcgatcg catcacgacg gatgttacga
    23041 atcgcgattt ttgcattttc tgcttctttt ttaacatctt tagctaattc ttttctgcgt
    23101 tcttctgtca gttgcgggat aactaagcga atgacatttc catcattggt tggactgatt
    23161 ccgatatcac ttgccataat agctttttca acatcttgaa gaatgctctt gtcaaaaggt
    23221 gtgatcatta aaacacgtgc ttctggaatt tggattgatg ccatttggtt caatggtgtt
    23281 ggcgcaccgt agtagttcac agtaatacgg tccaaaaggc tagcgttcgc acggcctgcg
    23341 cggatttgcc caagttcacg ttgaagattt tgtgctgctt ttttcatttt ttcttttgct
    23401 tctgtcataa ttgcatctgc catttttatt tcccccttac agttgttccg atattttcgc
    23461 ctaagattgc acggcgaata tttccgtgtt catttaaatt gaagacaagc agtggaatat
    23521 cattatccat gcttagtgaa cttgcagttg aatccatgac ttgtaatcct ttggcaataa
    23581 cttccagatg agtcagttct tcaaatttta ctgcatttgc atctactttc ggatcagcag
    23641 aataaacacc gtctacattg tttttagcca tcaggataac atctgcgccg atttctgccg
    23701 cgcgtaatgc tgctgttgtg tctgtcgaga agtacgggtt gccggtaccg cctgcaaaga
    23761 taacgatccg tcctttttct aaatgacgtt ctgctttacg gcgaatatat ggctctgcga
    23821 tttgacgcat ttcaatcgaa gtttgcacgc gtgtcggtac acccacgttt tccaacgtat
    23881 cctgtaaagc taaggcattc ataactgtag ctaacatacc catgtaatca gcttgagcac
    23941 gttccatgcc catttgtgct ccgatttgtc cgcgccagat atttccgccg cccacaacga
    24001 ttgccatttc aacgcccagc tcgtgaactt ctttgatttc ttggatgatt tctttgatta
    24061 ctggtggttt gattccaaat cctgcgtccc cagctaaagc ttctccactc aattttaaga
    24121 ctacacgctg atatttcggt gtgaccatgt tcattcctcc aatattttct attgccattt
    24181 taccatactt agatgatttc gacactctaa gactggtttt tcttgttttg cttaaaaaaa
    24241 gggagcgcac aaaccgtgcg ttcccgtctg ttattcaaca gaattatttt ttcacttggc
    24301 tcataacttc ttctacaaag ttatcttcgc gtttttcgat accttcgcca acttcgaaac
    24361 gtacgaatgt tttaacagtt gcgccttttg atgcaacgta tttttcaaca gtcatatcag
    24421 gatctttaac gaatggttgg tccaccaaag cgatttctgc tttgaatttc ttcaagcgtc
    24481 cttctaccat tttttcaacg atattcgctg gtttgccttc gttcaatgct tgttctgtaa
    24541 gaacagtttt ttcatgttct aattcagctt ctggaatttg tgattcgttt acataacgag
    24601 gattgatagc tgctacgtgc atagctacgt ctcttgcaac tgtttcgtca gttgttccat
    24661 ctagaacagt caatacggca atacgtccgc ccatatgcaa gtatccacca aatgcagcat
    24721 tgtcgtcttt ttctacaact tcaaagcgac ggaagctgat tttttctccg ataacttgag
    24781 ttgcttcgat caagtcagat tcaattgtac ctttatctgt tttgattttc attgcagctt
    24841 ccatgtctgc tggtttgttt tcagcaacaa gttctgcaat ttcttttact aaatcttgga
    24901 acatttcgtt tttagaaaca aagtctgttt ctgagtttac ttcaacgatt gctgcagtgt
    24961 tacctttcac agcaactgaa gccaatcctt cagctgcaat gcggtcattt ttcttcgcag
    25021 cctttgccat tcctttttca cgtaaaagat caactgcttt ttccatgtcg ccttctactt
    25081 caactaatgc ttttttcgca tccatcattc ctacgccagt catttcgcgt agttctttta
    25141 ccattttagc tgtaacgtct gccatgttta actcctcctg tgtgtttttc tttttaaaaa
    25201 aagctgcttc aaaggagagt gaggcttgtc gcccagcctt tgaaacagct ccatcattca
    25261 aaattattct gctgaagaat tgtcgccttc aacaacatct acgatttctt cgattgatgt
    25321 tgctgtgttt tcttctgtga aatcttcttc aactacttga tcttcacctt ggtttccttc
    25381 gatgaaagca tcagccattt ttgaagtaat caatttaacg gcacgaattg catcgtcgtt
    25441 tgaagggatt actacgtcga tttcatctgg atcgcagttt gtatcaacca tagctacgat
    25501 tgggatattt aatttgtgtg cttcttgaac agcaatacgt tctttacgtg ggtcaacgat
    25561 gtacattaca tctgggattc ttggcatatc agcgataccg cctaagaatt tttcaagacg
    25621 ttcacgttct ttgttcaaac cagcaacttc ttttttaggt agaacttcaa aagttccatc
    25681 ttcttccatt gcattgattt gtttcaaacg gctgatacgt ttttggatag tatcccagtt
    25741 tgtcaatgtt ccgcctaacc aacgatggtt aacgaagtat tgtcctgcac gagttgcttc
    25801 ttctttgatt gcttcttgtg cttgtttttt tgtacctacg aataatgcta cgccgccttc
    25861 ttcagcaacg ctcttcatgt aatcataagc tgcatctact aatttaactg ttttttgcaa
    25921 gtcaatgatg tagattccgt ttctttctgt gaagatatat ttcttcattt ttggattcca
    25981 gcgacgagtt tggtgaccaa agtgtacgcc ggcttctagt aattgtttca tagaaattac
    26041 tgccatgttt gtttcctccg atttggtttt atttttcctc ttcttatctt ctaccttgcc
    26101 ggcaaacttt ttcaagcacc taccgacaat ccaataagaa tgtggatttg ctgatgcata
    26161 aagcaccctc agctattata cagacaaaag atccgtatcg caagagcaaa tatacttttc
    26221 tgctcaacat tcaatagttt acctgaaaga taaaaagaat tcaagaaaaa cagtagattt
    26281 ctctttgttt ctttattatc ctttttccat aaaataattt taggtataaa agacgaatat
    26341 tattgggcaa tccttctttt ttttgttatg atacatgtga aatctttcgt aaaggagaaa
    26401 gaaaatgcgc aaagctcaaa gacatttgct gattaaacaa gtcattgaag aattcccgat
    26461 ccgtacgcaa gaagaactat taaacaaact tgggcattat ggcgttaccg ccacgcaggc
    26521 aacgatttct cgagatatcc gtgatctaaa aatcgtgaag gcacctgatg aaaatggcgt
    26581 ttcccgcttc gtattgtttc gcgaaaaagg aactcctgag actaggactg aggaagagga
    26641 tcggctggtt caaatgattg aagacattgt attgaaagtc gaacgagtcc actttttgac
    26701 gatcgtcaat accttgccag acaatgctca tttatttgca tctgttttag atgagatcaa
    26761 acctccctct attgtcagta caattgctgg attcgatacg atcatggtca tctcggaaaa
    26821 tgaggaggac gcaaaacgag tagaagaatt tcttcaccat ccaactgaag tatcactttt
    26881 ttaatcagag cacgtattcc taatatggaa tgcgtgtttt tttattttca atattttcct
    26941 tattgaataa acctttacac tctttacaaa aatacatcat tttttattca aatttttcaa
    27001 caccttgatt gaatcacaat aatcatcatt tatattggat gttaacaaga gcagcttctt
    27061 gctctttgtg tatttcaaaa aagaaatgaa aggaaatcat atgtataaat tattaacttc
    27121 tggaccattg acgacttctg aaacagtgaa aaatgctatg ctgatcgatc attgtacttg
    27181 ggatcaagaa tacaaagaaa ttacacaatg gataagaaaa aaattattgg atcttgctca
    27241 agtatcggaa ggaatataca caacggtctt aatgcaaggt agcagctcct tcggggtaga
    27301 atcggtatta agttctgtga tcaggccaga agatactgta ctgatttgtt caaacggcgc
    27361 atatagacaa cgaatgatca acatgtgcga atgtctgaaa ctgaactata ccgtttatga
    27421 ggtatcggaa catcaaatcc tccaagctca tgtgattaca cagctgattg aagagaatcc
    27481 gtctatctcc gcactagcaa tggtccatag cgagactact tccggtattt tgaataattt
    27541 ggaagagatt gctgctgtca cacaagaaag agatcttttg tttatagtgg acgcgatgtc
    27601 aagctttggt gggataccga tttgtgtagc cgaactaggg attgattttt tgatttccag
    27661 ctcaaacaaa tgtattcaag gtgttccagg cttttctttt gtacttgcta ataaagcgga
    27721 actggaaaaa cgaaaaaatg ttagccgaag tttgtcactt gatctatatg accagtaccg
    27781 cgtgatggaa aacgagaatg gcaaatggcg tttcacttcc cctacccata cggttttggc
    27841 ttttcaacaa gcgctgaagg aactagaggc agaaggagga atcgaaactc gatattcacg
    27901 ttatctcgcc aataatcatg tgctacgaca aaaaatgaca gagatggggt tccagccttt
    27961 cattcaagaa tatcaggggc cattcatcac ctcttttctt taccctaagg actggcgatt
    28021 tgattttaat catttttatc agtatttaaa gcaaaatgga tttgtgatct atcccgggaa
    28081 aatcagcgat catgaatgtt ttcgtatcgg aaatattggc gagatctatc cggaagatat
    28141 tttggaactg acagcaatta tcaaagacta taaggagaac gttaatgatt aaaactgtaa
    28201 tttttgattg ggcaggaaca actgtggact ttggctgtat ggctcctgtc cacgctttta
    28261 gaaatgcttt tctagaaaaa ggaatccaac tgactgacaa agaaatccgc taaccgatgg
    28321 gaaaattaaa atgggatcat atccaacagc ttctttccct cccttctgtc aaaaaacaat
    28381 ggatagctat ttatggacaa ctacctcagg aaaaagaggt ggaggaattg tatcaacaat
    28441 ttgaacatta tctgtttaaa gatttggtca agcattctac cttgaaaccc gacacattgg
    28501 aaacgatcga acggttgaga aacgctggga tcaatattgg ttccacgaat ggatttaatg
    28561 ctcctatgat gacaatcgta gcagaaaccg caaaaaaaga aaggtattca ccagacttca
    28621 tggcaacttt tgaagacgtc gaagggtatg gtcgaccgtt tccttatatg atccataaaa
    28681 acatgcagta cttcaaaaac aagtcggttg atgaagtaat caaagtcgga gatacggtct
    28741 cagatatcca ggaagggaaa aatgccggag tgttgacagt aggtgtaatt gaaggtagtt
    28801 cgctaatggg gatttcttat gatgagtacc aaaaattaga gatggggaaa aagatcgttt
    28861 aaaacagtct gttgctctta gattccaaca agccggcgct gatatttgtg taaacaattt
    28921 aaaagagtta acagaataca ttttgtacgc tgacatgaat ccagaatgac ttttatacaa
    28981 aataagaggt tgtgaaaaag ctgtcctgta tcaagtaata aaaacagcca agcaaaaata
    29041 gctcctcata tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg
    29101 tttattcgaa aaaaaaaagg gagctgtgac acgacttttg tcacagtccc cttatttgca
    29161 aacaaacagt gtttaagaga caacctctcc actaaacggt gttcattcag ctgactctcg
    29221 gtatttgctc aaaaaacgat aaaatttgaa aagctatttt ctcttttgtt tctctaatac
    29281 tcggatcaga acgctgattt cacattctct ttttcttaca tcttatctgg tgcatgaagt
    29341 cctagcaaac gaaggtcttc tttcaataat acagtcactg cataaactag agctaagcgt
    29401 gcttcttttt gttcatcatc agctaaaatc ttcgtatgag catagtattt gttgaatgct
    29461 tgtgctaatt tgattgcatg tttcgcaatc actgacggtt cgtatttttc cccagcagac
    29521 aagaccgttt cagggtattt ttgaactaat ttaaccactt cccagctggt atcgtcattc
    29581 aatgcatagt ctgctgtttc tgaagggaca aatcctgctt tttccaacag gctgactgca
    29641 cgagcatgtg tatattgcac ataaggtcca gtttctcctt caaatcgaac aacttcttcc
    29701 aggttaaagt caaatgtatt cagtcgatcg tttttcaaat cgtggaaaat gactgcccca
    29761 accccaactt gtttagctac agcatctttg ttttccaaat cagggttttt ctcgctgatt
    29821 tgttctttag cagattggat tgcttcattt aatacttctt ctaataagac gatctttcct
    29881 ttacgagttg ataatttttt accaccttgc gtgatcaaac caaatggaat atggtgcatg
    29941 tcgtcagacc agtcaaagcc catttccttc aagacagctt tcaactgttt aaaatgatag
    30001 ctttgttcat tccctactac atatagagat tgtttgaagt cgtaagtacg tttacggtac
    30061 aatgcagcag ctaagtcacg tgtaatgtat agtgtcgcac cgtctgactt cttaatcaac
    30121 gctggattca ggtcataagc agataggtca acgatctctg cacctttatc ctcgttcagc
    30181 aaatgttttt cttctaaaag tttgacgatt tcatccatct tgtcattgta gaaagcttca
    30241 ccgttcaacg aatcgaaacg tacttccagc agatcataga ttttattgaa ttctttcatc
    30301 gattcatcac ggaaccattg ccataattga atagcctctt tatcgccttc ttccagacgt
    30361 ttgaaccaag cacgagcttc ttcatttaat tctggttctg tttcagctac ttcatggaat
    30421 tggacgtata aacgaagcaa ttcattgatt ggttctgctt taacagcttc ttctgtaccc
    30481 cattttttgt aagcgacgat cagttttcca aactgtgttc cccagtcacc taaatggttg
    30541 atgcggattg gctgataacc gattttttcc ataatgaaac cgatcgaatt accgataact
    30601 gtcgaacgca aatgtcccat tgagattggt ttggcaatat taggagaaga catgtcgatt
    30661 ggtactgttc cttgattccc gatgttgcta tccccataat gttctttttc tttcactact
    30721 gtttgcaaaa cttttttgct gataagttct ttattcatga aaaagttcaa gtaaggtccg
    30781 acaacttcga ttttttcaaa gttggcagaa tcgatttttt ctgctaaatc tgcagcgatt
    30841 tgctgtggcg cttttcggta aacttttgct aatgaaaagg ctggaaatgc aacatctccg
    30901 tgttcagctg attttggatt ttctagtaat tgctcgactt gttccagtgt taaatcatct
    30961 ttgacagcgt catagacagc ttttgcgaca agatctttat tattcatctt tattcctcct
    31021 aaagtgttcc cttgattgtt aaacaaaaaa aatctctagc gacggcatac ttagtatgcc
    31081 atcactagag acgagatttc ccgcggtacc actccaattg tcctaaaaaa ggaccagctc
    31141 catctgatat cggtagacga ccgtctgttt aaaacagaat tctccaagtg cgcttcgttg
    31201 tatcttcttc attcggcttc caccacttcc gaattcgcta gccaagaatg atacaagtac
    31261 tctcttgttc aacaaatctt agtcttatta aactttcttg aattatatca gaaaaaaaaa
    31321 cgaaaggcta gtcgtttcat aaaaaaacaa tccagatggc ctaaaggaga agaaatacca
    31381 cctggatagc tataaatcat ttcgtaaata cgaaagactt aacaaattaa tcagctacga
    31441 taatcgttcc ttcttcagaa gctaaaagat tctcgatatt ttctaaagaa gtgatgattg
    31501 ccttagcgtt tggttttgct tcaacaaatg caatagctgc ttctactttc ggaagcatgc
    31561 ttcctggagc gaattgcttt tcatcaatgt attgtttcat ttctgaaaca gtaacagttt
    31621 ctaatttctt ttgatctggt ttttggtaat tgacatacac attgtctact cctgtcaata
    31681 cgatcagcaa atccgcatcg atgatttcgg ctaatttttc tgaagcaaaa tctttatcga
    31741 ttacggcttc tcgtccttct aatccagtag ctgtttctac aacaggaatc ccaccgccac
    31801 caacagagac tgtgataact ccttgatcga ccagcgtttc gatgacacgt gcttctttaa
    31861 tcgagatggg tttaggggaa gcgactactt ttctccatcc acgtccagca tcttctttga
    31921 atgtcacagt ggagtcagca ttcatttgtt ctttggcttc ttcttccgtg tagaatggac
    31981 cgactggttt acttggattt ttgaatgaag gatcattttc atccacaata acttgagtga
    32041 ctaaagaaac cacatcttta tcgatccctt tttccttcag tacttcgccc atggcgtttt
    32101 gtaaccagta accgatagat ccttctgtca ttgcaacaca tgtatctaaa ggcattgccg
    32161 gtgttttctc agaatcagca gcttgctgct gaatcaatag gttaccgact tgcggaccat
    32221 ttccgtggga gatgatcaat tcatctcctt gttcgataaa tttcactaaa tactttgctg
    32281 tttccattaa cgcttcttgt tgggcttttg cacttgcatc cgtagacaag atggcattgc
    32341 cccctaaagc gacaactact tttcgtttgg ccatttcatt cgtcctttct tcagttctac
    32401 cgaattttct agataacaaa acgagccagc gttttgtgat tttccctcaa tgctgactcg
    32461 ttttttcgtg agatgaatgt ggttatgctt aaacgcgagg gataaacaag ttacctaatg
    32521 ttgctgccat gatcgctttg attgagtgca tacgattttc tgcttggtca aattgacgag
    32581 catatttgct gcggaagact tcatctgtta cttccatttc agtgatgcca aagcgttctt
    32641 tcatttgttc accgtaaaca gtgtttgtat catggaaagc tggcaagcag tgcaagaaga
    32701 tcaaacgatc tgtgttgtgt gttttttcta ccatttccat attgatttgg taaggtttca
    32761 acaatttgat acgttcttcg aatttatctt cttcacccat tgatacccat acatcagaat
    32821 aaaggacgtc tgctccgtct acacctttat caacatcgtc ggtaatcatc aattgtgcac
    32881 cggatttttc agcaaagcct tctgccattt tgacgatttc ttcttcaggt tgtaattctt
    32941 ttggtgcaac gatacgcata ttcgctccta aaattgcacc tgtgactaat aaagagttag
    33001 ccatattgtt tcgaccatct ccgcagtaag caacagtgat accttctact gttccaaagt
    33061 tttcttggat agttaaaaag tcagcgatca tttgtgttgg atgccattca tcagtcaagc
    33121 cattccaaac tggaacacca gagaattctg ctagttcctc taccattttt tgactgaatc
    33181 cacggaattc gattccgtca aacatacgtc ctagtacttt agctgtatct tctgtggatt
    33241 cttttttacc taattggata tcatttgcac ctagatattc aggatgtgca cctagatcga
    33301 tcgctgcagt agtaaatgca gaacgagtac gtgtagatgt tttttcaaat aaaagcgcaa
    33361 tattttttcc ttctagatag tgatgtggga taccacgttt tttcaagtct ttcaagtgtt
    33421 ctgaaaaatc gatcaaatat tgtaattctt cttttgtaaa gtctttttct gctaataagc
    33481 ttcttccttg gaatactgat tctttcatat taaacattct cctttagatt gtttttattt
    33541 aagattaaag aggaaatccc gacggcagct gacagtaaga ctgccgtcag gaaacaaatt
    33601 acttcaagtc ttcacggaca agtggttggc tcatgcagcg tggaccacca cgacctcgtg
    33661 acaattcact tgaattgatt tctaacactt tgatgccata gctacgaagc aattcgttcg
    33721 atacataatt tcggttataa gtgactacaa cacctggagc gattgccaat gtgtttgatc
    33781 catcattcca ttgttctcga ggtgcaacga tctcatctcc attaccagtt gggatcagta
    33841 caagatcatc taatccaaga gcagctttca tcgtttcgtg taagttatct gaatgagtaa
    33901 tcttgatatc atcaccatct ggttcgattg taaagacatc catcttaccg tctttacttt
    33961 gaattgctgg atggatagtg aatttatcgt gatctaccat tgtaaatacg gtatccaaat
    34021 gcatcattgc acggttgttc ggaattttga ttgccaacac tttttcaaat cctgaatttt
    34081 ttgcgaatag gtggcgtgct aatttttcta aagctttcgc attagttcgt tgagaaatcc
    34141 caacagctac tactttatcg cttaagatca attcgtctcc gccttcgata tgatctggat
    34201 tttcacgatc caaccatact tcgattcctt tatttgcaaa tcttggatga tgtttcaaaa
    34261 tatattctgt aaacatcgat tcacgacgac gtgcttcaaa agtcatctta ttgactgtca
    34321 tcccatttcc cattgatgcg gaagggtcgc gtgtaaagta caagtttggc attgggtcca
    34381 tgtaaaatgg atagtcatcg tctgctgaca gatcatatag actgctagaa cgtacttcga
    34441 tatcttttgt acgaacccca gccatgattt tatctaccat ttcttgcgtt tccattgata
    34501 gaaggaactc atgaagaccc tctcttacag cattcgatgc aatatgagat tcatccaaca
    34561 ttttattcaa gaattgttct ttgacattgc ctgcgtcaat cgcttctgcc gctaattttt
    34621 ccaagtaaag agtctcgacg ccttcatttt gcaatgtctt cgcaaagttg tcatgttctt
    34681 cttgagcaat cggaagatat ggaatatcat cgaacaacag acgatccata atatctggtg
    34741 tcaaattttc cacttcttgt ccaggccgtt tcagtaaaac tgtctttaat ttccctattt
    34801 cagagaaaac gtgaataggt ttatccatgt tgtgttgcct ccttcttttt gattacatgt
    34861 ataggatatc ctccttttgt aatcactttc ataattttgt ctcattcaaa atgagacatt
    34921 cctcacatta atattcgata attaaataat taaacgtttt tctattttta agcgtaattt
    34981 attcattttt tattctgtat acactttcaa aaaaatattt attcattgaa aaaatttttt
    35041 tgaataaatt aaaaaaataa attatatta
//
LOCUS       DAZKBH010000017        34869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000017 DAZKBH010000000
VERSION     DAZKBH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.017"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACG8OO_000835"
     CDS             complement(59..187)
                     /locus_tag="ACG8OO_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9044759.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8OO_000836"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8OO_000836"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9044760.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACG8OO_000837"
     CDS             complement(1674..2729)
                     /locus_tag="ACG8OO_000837"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9044761.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8OO_000838"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8OO_000838"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFP9044762.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8OO_000839"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8OO_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFP9044763.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8OO_000840"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8OO_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFP9044764.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8OO_000841"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8OO_000841"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9044765.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8OO_000842"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8OO_000842"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFP9044766.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACG8OO_000843"
     CDS             10491..12050
                     /locus_tag="ACG8OO_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFP9044767.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACG8OO_000844"
     CDS             complement(12161..14092)
                     /locus_tag="ACG8OO_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFP9044768.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8OO_000845"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8OO_000845"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9044769.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACG8OO_000846"
     CDS             complement(15021..15773)
                     /locus_tag="ACG8OO_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9044770.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACG8OO_000847"
     CDS             complement(15932..16141)
                     /locus_tag="ACG8OO_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044771.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACG8OO_000848"
     CDS             complement(16152..17480)
                     /locus_tag="ACG8OO_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044772.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACG8OO_000849"
     CDS             17616..17771
                     /locus_tag="ACG8OO_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFP9044773.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8OO_000850"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8OO_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9044774.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8OO_000851"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8OO_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFP9044775.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8OO_000852"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8OO_000852"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9044776.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACG8OO_000853"
     CDS             complement(21455..22387)
                     /locus_tag="ACG8OO_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044777.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACG8OO_000854"
     CDS             complement(22544..23203)
                     /locus_tag="ACG8OO_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFP9044778.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8OO_000855"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8OO_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9044779.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACG8OO_000856"
     CDS             complement(24244..24534)
                     /locus_tag="ACG8OO_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044780.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="ACG8OO_000857"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="ACG8OO_000857"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9044781.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /gene="glmM"
                     /locus_tag="ACG8OO_000858"
     CDS             complement(26820..28175)
                     /gene="glmM"
                     /locus_tag="ACG8OO_000858"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9044782.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="ACG8OO_000859"
     CDS             complement(28199..29368)
                     /locus_tag="ACG8OO_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFP9044783.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /gene="cdaA"
                     /locus_tag="ACG8OO_000860"
     CDS             complement(29365..30252)
                     /gene="cdaA"
                     /locus_tag="ACG8OO_000860"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFP9044784.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="ACG8OO_000861"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="ACG8OO_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9044785.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="ACG8OO_000862"
     CDS             34332..34853
                     /locus_tag="ACG8OO_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFP9044786.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9400 a         7569 c         6284 g        11616 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtg
//
LOCUS       DAZKBH010000018        31622 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000018 DAZKBH010000000
VERSION     DAZKBH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.018"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(208..2100)
                     /locus_tag="ACG8OO_000863"
     CDS             complement(208..2100)
                     /locus_tag="ACG8OO_000863"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044787.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2097..3824)
                     /locus_tag="ACG8OO_000864"
     CDS             complement(2097..3824)
                     /locus_tag="ACG8OO_000864"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044788.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3840..4490)
                     /locus_tag="ACG8OO_000865"
     CDS             complement(3840..4490)
                     /locus_tag="ACG8OO_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFP9044789.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4694..4990
                     /locus_tag="ACG8OO_000866"
     CDS             4694..4990
                     /locus_tag="ACG8OO_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9044790.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5162..5782
                     /locus_tag="ACG8OO_000867"
     CDS             5162..5782
                     /locus_tag="ACG8OO_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9044791.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5874..7040)
                     /locus_tag="ACG8OO_000868"
     CDS             complement(5874..7040)
                     /locus_tag="ACG8OO_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9044792.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7052..7405)
                     /locus_tag="ACG8OO_000869"
     CDS             complement(7052..7405)
                     /locus_tag="ACG8OO_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9044793.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7533..8555
                     /locus_tag="ACG8OO_000870"
     CDS             7533..8555
                     /locus_tag="ACG8OO_000870"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFP9044794.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8574..8735
                     /locus_tag="ACG8OO_000871"
     CDS             8574..8735
                     /locus_tag="ACG8OO_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044795.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8994..9428)
                     /locus_tag="ACG8OO_000872"
     CDS             complement(8994..9428)
                     /locus_tag="ACG8OO_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044796.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9538..11397)
                     /locus_tag="ACG8OO_000873"
     CDS             complement(9538..11397)
                     /locus_tag="ACG8OO_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9044797.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11398..11538
                     /locus_tag="ACG8OO_000874"
     CDS             11398..11538
                     /locus_tag="ACG8OO_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFP9044798.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11515..12675)
                     /locus_tag="ACG8OO_000875"
     CDS             complement(11515..12675)
                     /locus_tag="ACG8OO_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9044799.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12982..14406)
                     /gene="araA"
                     /locus_tag="ACG8OO_000876"
     CDS             complement(12982..14406)
                     /gene="araA"
                     /locus_tag="ACG8OO_000876"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9044800.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14422..15120)
                     /locus_tag="ACG8OO_000877"
     CDS             complement(14422..15120)
                     /locus_tag="ACG8OO_000877"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9044801.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15077..16738)
                     /locus_tag="ACG8OO_000878"
     CDS             complement(15077..16738)
                     /locus_tag="ACG8OO_000878"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9044802.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16956..18356
                     /locus_tag="ACG8OO_000879"
     CDS             16956..18356
                     /locus_tag="ACG8OO_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFP9044803.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18608..19687)
                     /locus_tag="ACG8OO_000880"
     CDS             complement(18608..19687)
                     /locus_tag="ACG8OO_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9044804.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19773..20732)
                     /locus_tag="ACG8OO_000881"
     CDS             complement(19773..20732)
                     /locus_tag="ACG8OO_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFP9044805.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20744..22255)
                     /locus_tag="ACG8OO_000882"
     CDS             complement(20744..22255)
                     /locus_tag="ACG8OO_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFP9044806.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22277..22840)
                     /locus_tag="ACG8OO_000883"
     CDS             complement(22277..22840)
                     /locus_tag="ACG8OO_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9044807.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22846..23709)
                     /locus_tag="ACG8OO_000884"
     CDS             complement(22846..23709)
                     /locus_tag="ACG8OO_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9044808.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23723..24619)
                     /locus_tag="ACG8OO_000885"
     CDS             complement(23723..24619)
                     /locus_tag="ACG8OO_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9044809.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24794..26083)
                     /locus_tag="ACG8OO_000886"
     CDS             complement(24794..26083)
                     /locus_tag="ACG8OO_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9044810.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26055..27572)
                     /locus_tag="ACG8OO_000887"
     CDS             complement(26055..27572)
                     /locus_tag="ACG8OO_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFP9044811.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27770..28660
                     /locus_tag="ACG8OO_000888"
     CDS             27770..28660
                     /locus_tag="ACG8OO_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFP9044812.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28733..29557)
                     /gene="nadE"
                     /locus_tag="ACG8OO_000889"
     CDS             complement(28733..29557)
                     /gene="nadE"
                     /locus_tag="ACG8OO_000889"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9044813.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29569..31041)
                     /locus_tag="ACG8OO_000890"
     CDS             complement(29569..31041)
                     /locus_tag="ACG8OO_000890"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9044814.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         9255 a         6636 c         5468 g        10263 t
ORIGIN      
        1 ttttgaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga gtattagaga
       61 tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata ccgaaggaca
      121 gctgattgaa caccgtttat tcggaaaaag ggactgtgac acgacttttg tcacagcccc
      181 taaaaaaaat aataaatttg tactattcta tgaagcttct tcttcagaaa actgactgtt
      241 ataaagatct gcatagaatc cgtctttttc catcaatgta tcgtggtttc ctgtttctac
      301 gatcgaacca tgattcatca caataatgtt gtctgcatcg cgtattgtag acaagcggtg
      361 agcaacaacg aaacttgtcc ggttttccaa taggcggttc atcgcttttt gaatcagtat
      421 ttctgttcgt gtatctacac tagatgttgc ttcatcaagg atcaggacat ctggatttgc
      481 taagaatgcc cgcgcaattg tgattagctg acgctgccct tgcgaaatat tgcttgcatc
      541 ctcgtttaat acagtgttat atccttctgg caatttacga acaaaatcgt ctacgtgagc
      601 agctttagca gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat atttgatatt
      661 gtcatagata cttcctgtaa acagccaagt atcttgcagt accattgaaa actgtgcgcg
      721 caattcatca cgtgaaagat cacgggtatc tacaccatcg taacgaatac tacctccaga
      781 aacatcgtag aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc
      841 tacaatagcg accatctcac caggtttgac atccaagtta aaatcagtca ttagcaagtt
      901 atctggtgtg tagccgaatt gaacattttc aaaacgaact ttgtatggtg tatctttttc
      961 ttctggaagg ccagattttt cattggacat ctcttcttca tccaacactt caaatacccg
     1021 ttcggctgaa gcaactgttg attggatagt gttcatcaaa cttgcaagtt gagtgattgg
     1081 ttgagagaac tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg tcatgttccc
     1141 atttgcaact tttactccgc ctagtacagc gacgaacaca tagcctagat ttttaacgaa
     1201 attcatcaac ggcatgatga ttgccgaaat aaattgagct ttccaaccag cggcatacag
     1261 attctgattt tctttttcaa atacttcctg gtctttttca gtatgattga atgttttaac
     1321 aatggtatga ccaccatatg tttcttctac ttggttattc aataaaccta aacttttttg
     1381 ctgatctgcg aaaaacactt gtgatttcgg cgctacgatc atcacgatga tcaagcttaa
     1441 agggatcatc aataacgcga ctaacgtcaa ttgccagctg attgaaagca tcatcacgat
     1501 gaccccaatc aatgtgactg cactagttac aaattgagtc aaattttgct gcagcgtgct
     1561 tgcgatgtta tccatatcat tgattgctcg agacatgata tccccatttg tatgtgtatc
     1621 ataataggaa atcggtacac gattcatttt ggcttccaaa tcatgacgta attcataaac
     1681 tgttcgttgg gaaacacgtg tcataataaa ttgctgtaaa aagttgaata cagcagaaat
     1741 caaatacatc acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa
     1801 ttttgttact tgttggcctt gttgcatctg ttgagcgcca gccattacac ctttgaatat
     1861 ttctgtggtt gctttcccca agacctttgg tgtttggatt tggaagatca cggctgctat
     1921 tgctagtaca aatacagcga tgatcgcaac catacgttta gacatatagc caaacaatcg
     1981 gcgtaccgtt ccccagaaat ttttaggctt ttcacctttt acaccgagat ttctgccagg
     2041 tccaccatgt cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg ttttactcat
     2101 gctaagtcct cctctcttag ttgtgaatga acgatttctt gataagtttc gtttgttgct
     2161 aatagttctt catgtgttcc tttgccaacc agttttcctt catctagcac aaggatcata
     2221 tctgcatcca ttactgtact tactcgttga gcaactaaaa cggtgatact ttctttcata
     2281 ttcgttttca atgcttggcg aagattggca tccgttttga aatccaacgc tgagaaggaa
     2341 tcatcaaaca catagatatc tgctgttttg actaatgccc gtgcaattgc cagacgctgt
     2401 ctttgtccac cagagaagtt tcctcctcct tgttccacat ggctatctaa gccatcttcc
     2461 atctctgaaa caaaatcttt agcttgtgcg atttctaatg ctttccaaat catttcatct
     2521 gttgcatccg gcttaccata ctgcatattg tctcggatcg ttccagagaa cagaactgct
     2581 ttttgaggaa caaagcccat taattctcgc aaattatatt gagtcatgtc actaatgtcc
     2641 gttccattta tttggatcga accagattcg atgtcatata atcgagggat caaattgact
     2701 aaggtcgttt tacctgaacc agtcccccct atgattgcga cgatctcgcc cgacttggct
     2761 tggaaatcaa tatcttccaa tgcaagtttt tctgctccat gatatcgata attgacatga
     2821 ttgaaggcca atgttgcgtt ttttccagca aaagaaactg ttttaggatt ttcagggtca
     2881 tggataccga ttttttcatc caacacttca ttaatacggt cagctgaagc ttgtgcacga
     2941 ggcaccatga taaaaatagc ggaaagcatc ataaagctga tcaagatctg catcgcataa
     3001 gtcataaatg caatcagatt accgacttcc aaagtcatat ctgctatgta gtgtccacca
     3061 aaccaagtga ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg catcatcaat
     3121 gcgacgatcg tattaacttt gattgccgta tttgtataat ctttattcgc taaatcaaaa
     3181 cgattttctt caaagcgtgt cttatcgaat gctcgaatca ctctgacccc tgtcaatcct
     3241 tctcgaaata ccaggtttaa acggtcagtt ttcttttgca tacttttgaa taatggaaca
     3301 gcaaagtaca tgattccgcc gatcaaaata atcattactg ggatgacaac taagaagatt
     3361 tttgtcaact gatgatcttt ttgataagca agtatgctcg cgccaatcaa cgtgattggt
     3421 gcattgatca tcatacgtaa aaacatttgt gtaaccattt ggatttgagt cacatcattg
     3481 gtcgttcttg tgatcaaaga ggccgttcca tatttatcaa acgcatcttt tgtcagactc
     3541 tctgattttt tatagacatc cgtacgcaat tgcttaccca gtttctgtga ttcacgagta
     3601 gcaaaaaatg tatttgcgat agcagctaag atactgatca aagaaaagcc gatcatcaca
     3661 aaacctgtat gccaaatata atcgacatct ccttgcgcta cccctttatc aataatattg
     3721 gaggtaagtg tcggcaaata taaatctgcc agtacttgga tcaccatgaa aactgcagct
     3781 gcaattgctg aagtcaagga gatccgtttt acgattttta gcatctagtt cccttctttc
     3841 tatttatata ctccgttttt cagccaagaa agtttcaaat tgaattcact gattgcttgc
     3901 tctactgagt aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat
     3961 acagtattca tcactaattg gatcagtaat tccaattcat gatcattttt tactttcagc
     4021 ttcgtccgat cgatcaattc aagcaactct tttgcatctt gtttgtgttc tttatgtgga
     4081 tcgtgaggcg ttttttggcg actcgtatga ggagcgactt ttcttgaggc acggtaatcc
     4141 atgttcataa acaagttacg ataaaaagaa gcattctcgc cttccaaaat ctcataaatc
     4201 attttagaaa aataggattc gaatcctttg aatagatcac catcggattc tttcattagt
     4261 tgtaacatat ctttagagct gtcacgtctc attgtctcaa agtaataaaa ataaagatcc
     4321 tctttatctt caaaatactg atagaaactg cctctaggga tatccgctaa tttgacgata
     4381 ttagcaatcg aagcttcttt taagggtact ctagaaaact cgattctcgc agcctcgatt
     4441 agtctttttt gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat atggatcctc
     4501 ctttctttca agatgacaca ttgtcatatg acgctgtgtc attataacac ctcctacctg
     4561 ttttgcaaga catttgcata gtttttttca ttatcatccg atacattaaa aaagtgataa
     4621 gaggaatcct tccatttgaa ttaatgagag ttgcccatta aaatagccac atactaaaca
     4681 aaggatgaga taaatggccc aatcaattag tgtccaagac ttcaataaat tgcctctcga
     4741 tgctgacagc atcgttttag atacaagaaa taccttagat tacacaaaaa accacttaag
     4801 ccactcgctt tctatccccg ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa
     4861 gaaactttat tatattctaa gctatacagg caggcgatca gaagcattag ccgatcaact
     4921 tactgctaat ggttttcgtg ctgtttatat cattggtggg atggaagctc ttcagtcgag
     4981 tgttgcttga atattgcttg ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag
     5041 cctcttttat accctgtctt tttttagtta tttattcttc aaccttatca atatgacttg
     5101 atagcctttt ctttttctca tagttataac aatcatagca ataaaaggag aaggtaagca
     5161 tatggcatct gttttagtag tcaaaggtca tcccctaact gcagaagaat cgcgcacagt
     5221 taaggcattg acgtcttttt taacaagtta taaagaaaat catccagatg atgaagtgac
     5281 tgttttggaa ctttatcgcg atgatattcc ggaaatcgat gaagaattat tatcaggttg
     5341 ggaagcatta cgtgctggtg cagaattcac ttccttatca gaaagtcaac aacaaaaaat
     5401 tgctcgtttc aatgaattga ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa
     5461 cgccttatgg aatctaaata tcccaacaaa attaaaagct tggttcgata ccgtcaatgt
     5521 agctggaaaa acgttccgct atacagaaaa cgggccagaa ggattagtga caggtaaaaa
     5581 agcactgcac atccaatcta atggcggtgt atacaacgga caagactttg cctctcaata
     5641 tgtaaaaggt attttaaact ttgtaggcat cgatcaagta gatcagctat tcatcgaagg
     5701 aatcgattat gaccctgacc gtgcagatga gttgatgcaa aatgcattag ataaagctgc
     5761 tgctttaggt aaatcatttt aaaaattgag ataaatgcat acaaaagaga cagaggaaaa
     5821 aatcttcaaa gcagcatttg taccttggat attttcccat ctgtctcttt ttgttattct
     5881 ttctgctttt tcaacggatt ttcttcataa agagaagaga ttgttgtgta ccattcaaaa
     5941 acatgagtaa tcactacttt agctgctgca taaacaggaa tccccaagat cacaccgatg
     6001 attccgaaca atttcccaga agtcagcaga actaataaaa tcgttactgg atggattgct
     6061 aactgattcc ctagtactaa tggagagatc aagcgtcctt ctattgtctg ctctactgca
     6121 aaaacgatca gtactttgat gatcatgaat ggacctgcta caatggctaa aaagatagcc
     6181 ggaatcatcg ctaaaaatga tcctaagtaa gggatcaggt tcaaaaaacc cgcaataatc
     6241 cccaacgtga cagcatactc taaaccaatg accgcaaaac caatcataaa catgattgcc
     6301 acagcaaaag ccacagttag ttgtcctctt atataagaag aaacttgact attcatttca
     6361 ctcagcacat tcaacgtcgg tttttgcatg cgtgtcggta aaaatttgac aaaataaggt
     6421 gctaattgtt taccgtcttt caatagataa aataagataa acggcatcgt cagcagagcc
     6481 actaaaatag tcgctaccgc gccgacaaaa cttccaagac tctgaactgt actcttagat
     6541 atatcttgga tcatatcgcc tgccgaattc atgaacttat cacctgctgt ttccaactgt
     6601 tcgcgaaatt ggttgaacag cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta
     6661 tctacatatt gagggaagtt actgatgaag ctcactgttt gttcctgaat cttagggatg
     6721 atcactacgc tcccccatgc gattagtgca acaaccaaaa caaacagccc aatgatactg
     6781 taaagccttg gtacttttcg cttttcaaag taatcaacta ccggattcat caagtaatac
     6841 aagattcctg ctaaaatgat aggcagacct acgattgcta aaaattgcca gattggtgca
     6901 aacaaatatg aaacttttgt aaataaaaag acgatcagca atattaacaa tacaacaagt
     6961 aaggctgtga ctacttgatt atttaaaaac cagcgccaaa accacgatac cggtctgttc
     7021 ttcttatttt ttccttccat aattcctcct gttagttata aaaaatttgg cttcccaaac
     7081 caatagcagg cagtctatgc ggatgaagat agattccatt tgcaatttgt ccttcttctg
     7141 gacaagcatc aaaaatcaat gatacgtgag ctactgtctc taaattccca ttggccatcg
     7201 gaccgacata atcgatcgta tatacctgat catcaaaagc aattgttccg cctgctttca
     7261 aagaaaaagg ttcgtctttt gatacttctt gaatcaccga atattttcgt aaggcagctg
     7321 ttgctgtttt atcgaacaaa atcagcatgg gttcttcctt gtttattgct tctgtcccaa
     7381 tttctgtaat ctttgcctga atcattgcac tctctccttt aattgctttc aaaaacagta
     7441 taacacattc tcattcttta gccgtgtgga ataattatgt gatagaataa aaacgaaaac
     7501 tatttcatta caattaggag gaatcagctc atatgatcga aaagataatt ttaggaacct
     7561 atacgcgtcg cgaaagtaaa ggaatctata cgatcgacct tgatacagaa aaagaagaat
     7621 tagccaacct ttcattagct gttgaagaaa atagccctac ctatgtagca aaaagtaaag
     7681 ctggcaatct ttacacagtt actagtgtgg atggattagg tggtgccggt gcgtacgata
     7741 aggattttca ttttttgaat gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg
     7801 ttgatgaagc acgtcaatta ctatacgggg cgaactatca taaaggtgaa gtaaatgtct
     7861 accatatatt aggagatggc gaactgaaag cagcagattc aatattccat caagaagcga
     7921 caggtcctca taaaaaccaa gatcatgcgc atgtccacta tacggatctg actcctgacc
     7981 aacgattagt cgtatgtgat cttggtacag atcgtgtgta tacttatgat gtgtccgaaa
     8041 acggcaaatt gaatcttgta actaccttca ctgcagagcc aggaacaggc cctcgccatc
     8101 ttgtctttca tccaaatgga caattcgctt atttatttgg tgaattagac agcacagtcc
     8161 gcgtgctttc ttataataaa gaaacaggtg cttttgaaga attacagaaa atatcgacct
     8221 tgccagaaga attcgacggt gaaaatggag gggcagctat ccgcatttca aatgatggca
     8281 agttccttta tgcttctaac cgaggacata actcgattgc tgtattcgca gtttttgaag
     8341 atggttccca cattcaaaac aagcaaatca tctcaacaga aggggatttt ccccgcgatt
     8401 tcgcactaga tccaagcaat aattttgtcg tgtgtgccaa tcagaacacc gataatctga
     8461 cattatatcg ccgtcatcct gaaacaggat tactggaatt actgaaaaaa gatgtctatg
     8521 taccagaatg tgtctgtgtt tattttgatc gttagtaaga aaaggagaat gaaatgaatc
     8581 agcaagacgt attagacaga ctacgggaag aactaaatat tcctttcttt gatgggaaaa
     8641 tcgaagataa agagtatagc gaagaggaat atcagaaact caaagccgat ttacagaact
     8701 actttgaaca atatgtacag aatgtagaaa actaatcagc tttccatact atctgaaaag
     8761 aacatcagga caagactttt ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc
     8821 cgagatggtt agactcgacg gaaaaggagt atttactcga ttttgagtaa gtacaaggtt
     8881 gttgttggtt ataggtatgt cattatcata aaggattttg atcactctat cataaaggta
     8941 atattacatt tatattagat aactctagtt tttattgtta tcaaagattt cttttatttt
     9001 ttcaacaaca aattagtcat tttttggatg gcagctgtca attggtctgt cgcttggtct
     9061 tgttttgctt cagatgtatt gaatagatct tcatctaccc ctgattcaag atcgatactt
     9121 tggatcattt cttcacagcc tgctacatat gaaagataag ctttttcaaa atttttatga
     9181 atgcccattg cttttgctgg tggacgcaaa gtactgactt tcttaagcat tgcccgatat
     9241 ttttttgttc cttcttcaaa taacaggatg atctcttcaa gagtcgattt gtccagttca
     9301 cttgtttttc cttcatcaat tgcgtttctg atcacttcat atttcggatg cattccttct
     9361 ccgattgctt ctgtttcctt gacgatttga tcgatggact gcacataata gccaatattc
     9421 ggtaacatgt tcgttttcct actttctcca ttgttgttat tatttattct acttgttttt
     9481 atcctcaaat gacaagtact tgaaaaaatt taatggtttc tatacagctc gccattctta
     9541 ttcgtgctca aagaaaccat ctgctaaact atattgtttc cctgcgacaa tgtcatattg
     9601 gatcaattga tattcgtcgt aaatctcttg ttgcgagcga ttgaattgcg cttctttata
     9661 ccactgtccg ttttgatagt acagttctcg ttcaaacgcc ggaagttcct gttctaatga
     9721 taaaagcagc tgataaaagc catttgttgt ctgattcgtc tgttccatta gattcgctgc
     9781 aaaataaaac ggactggtga ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact
     9841 gtcgaacata agaaattgtg tttcatgcaa tgtttgtttg tcattttttg cttggatcgt
     9901 atctgaatag atccctggaa gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag
     9961 attcttcaat tcttccgtga actgcttcag tgccgtgctg ctataggaaa tatcttgcag
    10021 ataattctct aagtcaagtg tcccacttcc atctgaagtt tttgcaggat agtctagatt
    10081 atcatacttt ccatcgtagg gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg
    10141 tgttttttct tccttgagaa gtgtcattac ttctttgtaa gctgactcat cagaaatata
    10201 tggattattc tctatcgtat ccgtataggt catcgtacgc tcgctgatga actggtcaaa
    10261 tcctaatgtc tgataaacat cctctctttt atacatcgaa gtattgtatg gatggatcgc
    10321 tgtcgtctgg tatgattgtg cgtttaacgc tgaaacgata gaaggaagct gatccatttt
    10381 tggtacaagc atcgtgtaag gggtggtgag ttgcgcatta aataacgcca tagaaaaacc
    10441 agtcaatgct tcaaattcga tatttgctgt tccaccgcca tagttttgag aaagcatatt
    10501 gccactatat gtttgatcag ctacttcata ataatctgcc aatggctcac cagatacttc
    10561 tacgccgttt aatcgactag gatctgagaa actttcactc atcacgaaca cgatgttcgg
    10621 cgactcttcc tctacagttt tatttttttc atcggctagc ttctggtatt tttcagtgat
    10681 ttctttgatc ttttctttcg aatatccttc aggttcatcc attggttcta ccttcagatt
    10741 gtacaaaaac ccaccgataa aaccagtgtt atagtagttc attttctggc tgtaaggtat
    10801 ccatagtgcc gttttattat atgcttttcg caacaaatta tcgggattgt tgaaattact
    10861 gatataccct agcagtccaa ttgccacaag cagtgtcgtt aatcggatca attggaatcg
    10921 ccttcctttt ttcaggcttc gaaacatgta ccagcaaaac aatcctatca cggttcctgc
    10981 ggcagctaag atcactgtaa acagcatagt gccggtcata tctttcagca atccgatttc
    11041 agtgatcatc ttcaaatcgt ctggataaat cggttcttgc cgataactca ttttcatata
    11101 atttgcaaaa cctaatacac cgatggctac gacatacgta agggaaccta aagggacaga
    11161 tcctgctagt gcaatcagaa aaataagaat aatcagcaag acgagacagg ctaagaagaa
    11221 tttttccgta tgccaagaaa aagcaaattt caacgcaagg tctacggata gatcattttg
    11281 acaccactgc aaaaataaat tactaaaaac aacaagaaaa aagatactga tgattgctgc
    11341 tgttcctttt aaaatctttt tctgtttggt cgaaagtctg cttatatatc ttttcaaatg
    11401 cttgctcctt catttctatc aataaaagaa tgttgcttct cttattttag ttgtacgaaa
    11461 ttcgaaagta aagaacggaa agaagaatta atgaaatcca aatattctct ttctttatgg
    11521 cctttctggt ttctttaata agcggacctt tcgtactcca gaaatttttt ctagctgttg
    11581 tttcacttga ttgagctgtt caaaatcagt actgtctatg tcaatcaata tgtagacatg
    11641 ctcattctta tggttacgcg atatatctgc gatcgctagc tcgttctcat tcagcagaga
    11701 aaatatctct gctaatatat tggttttctt ttgataaaat atagtgaatc gaaacggcgc
    11761 ttggaaaaac attcgtgcgg atggataatt gaccgcttct ctaaccgtac cgaacaaaag
    11821 ataatctttt aaggcttcaa ctgccaaacg tccgctgtct gccaaagctt cctgagtcga
    11881 tccccctaga tgaggcaaca taagtatacg gtcattttct aagaactctt cttctggaaa
    11941 atcagtcaag tatttagcca gatgttgttt ttccaatgct tgcataacag catatttatc
    12001 aacaatttca cttcttccta cattgataag tacagcatct tttttcattt tttctatatt
    12061 cgcctgatcg atcaatcctt ttgtatcttc tgtcaacggc agcatgacta cgatgtaatc
    12121 cgaggtagaa agaactgttt ctaaatctgc ttgccgaaca taatccaact gggcatcgcg
    12181 gatagaataa cccaagacat ccatccctaa gctatagcaa cttagcgcta cttttgtgcc
    12241 tatcgctcca agaccaagaa gaccgatggt tttgccttgc aattctcgtc ccacataagc
    12301 acttcttttg ttttctgcct gttctaaaat attaggacct gttagtgttt gtaccatacg
    12361 acttgcttcg attatcggac gggaagataa gagcaaacag cacaaaacaa gctcttttac
    12421 tgcattagca tttacacctg gtgtattcat gacgatcgtc ccgttttcag aagctttttc
    12481 aacgttgatc gtgttcaccc caacaccagc tcttgaaatt gctagcaaat ctggtgtaag
    12541 ccattcatct ttcactttcg tactacgaat aactattcct tcggctggca aatcatctat
    12601 tattttgaaa tattcccctt gtaacttttc tatctcttgt tctggaatag catcaatcaa
    12661 atgaacgtga tacataaaac cccctcctta ctaacaagta tctcgaaatt tctgcaatta
    12721 gacaactgtt taccatttga cttcctagaa gcaaaaagag cagttagagc agagtaggct
    12781 gagtgataaa aataaggcta tgacaagtta ctgccataag ccttattttc gagagcaaaa
    12841 tattgagggt gagatatctt gataaatggt gttccgttag ctgacattga taataagact
    12901 aaatttcaaa aaagttgaaa agcaaatttc gtcaatctct tcttatttct taaaactaag
    12961 tggctttttc acaacctctt tttatctaat aaccactgtt tccaactcaa ctaattttgc
    13021 ccacgtttca atttgatctg ttgttagatt tagcgagaca acggtatgat gaccgccacc
    13081 tgcctgaatc catgaatgga caccttcatg gaaattcggt ttagttttcc ataacacacg
    13141 tgcaacaggt aaattaggtg cttcagtcgt tggttcaaag gcttctactt cattgatcag
    13201 cagtttataa tgcgtaccga aatctgccat cgatacgact accccttctc ctgctttccc
    13261 atcaaataca agacgcgccg gatcttctct atctcccatt gataaaggag aaacaacgat
    13321 tcttggtttc gtcgcagcaa gcgtcggatc tacttccatc atatgggatt ccaagattgc
    13381 ttcttggcca ctagctagct cgtaagtata atcttccatg aatcctgtat ctttccctcg
    13441 agccatgatt ttcagcaacc gatcaatcgc tgcagttttc cagtcgcctt ccccagcaaa
    13501 accgtatcct tctgccatca aacgttgtac agctagtcca ggtagttgtt tcattccata
    13561 caggtcttca aaattagtcg taaaagcgtt atagcctcct tcttctaaga aacggcgaat
    13621 accgatttcc tgctgagctt gtacttttac atgttcctcc catgtctttt cttcataatc
    13681 accatagtcg aaggtataca attccttgta ctcttcaaat aattgatcga cttcttcgtc
    13741 tgatacgcga tcgattactt gtacaagatc tccgatacca aaataatcaa cagtccaacc
    13801 aaattgaatc tgtgcttcca ctttgtctcc ttctgtgaca cccacgtttc tcatgttgtc
    13861 accaaaacga gccactttta ttccaaaact ttcattatag gctactgcaa catccatcca
    13921 atcagcaatc tctttttgga tctcaggtcg tttccagtag ccaacgacga ttttattttg
    13981 tttatttaat cgagcattga tgaacccata ttcgcggtca ccatgagcgg attgatttaa
    14041 attcatgaaa tccatatcga tcgttttcca aggaatgctt tcgttatatt gcgtagctaa
    14101 atgaagcaat ggtttttgta gaagctttgt cccgcggatc cacatttttg ctggtgagaa
    14161 agtatgcatc caagtgatca cacctgctac ttcttctcga taattgacct ctttcatgat
    14221 ttttgtgatc gtatcagcag taactgctaa ttcttgaagg cggattggat aaggcaattg
    14281 gccattttca ttcagttcat cgaccatttc ttgtgcatgt ttctttactt ctcgcagtgc
    14341 ttcttcccca tataaatgct gtgatcctac aacaaaccaa aattcttttt ctccaatatt
    14401 caacataatt agccactcct tttatttgtt ttgtccgtaa tacgcatttt ttccgtgttt
    14461 tcgtaaataa tgtttatcta aaatccgttg aggcagctct tctgaaaatg aattgagctg
    14521 tctggtaaat aaattcatct tgcatacttc atctagtacg actgcgttca ttaccgcact
    14581 ttgcgcatct ttcccccaag taaatggacc gtgtccgtgt aaaagtaccc ctggaattgc
    14641 taagggatca attttgcgtt ctttgaatgt ttctatgata acttttcctg tttcatattc
    14701 gtacccgctg tcgatttcct gttgtgtcaa aaatcgagca caaggtaccg atccataaaa
    14761 agtatctgca tgcgtcgtcc ccatcgcagg aacatccaat cctgcttgtg cccagatcgt
    14821 cgcccatgtc gagtgagtat ggacgattcc tccgatttga ggaaatgaac gatataaaac
    14881 agcatgagta ggcgtatccg aagagggatt gagtttacct tcaatgatat gattgtccaa
    14941 gtccacaacg accatgtcgt ctgctgtcat ctcttcataa ctgactccgc taggtttgat
    15001 aacgaaatat cgtgtttcag gatcaaaagc gctcacattt ccccatgtat atttgacgag
    15061 tccgtgttta ggcaagtcaa gatttgcttg gtatacttct tctttcagtt gttccagcat
    15121 tttctatcac tcctttttct tgcaattgat gaatcgctgt tttttcaatg tcgatcccct
    15181 cacggtaacg tctcataaat atctgatagc ctgctatttc ttctgctgaa ggtgtgatgg
    15241 tgattccttg gtcttgtcca aacacgtctt ctgccaagaa gttttctaaa gacttcccat
    15301 gtcgatccat catataggct gctaataacg cgattcccca cgctccacct tctccggctg
    15361 tgtccatcac tgtaactggt gcctccatag cagatgctag aatccgctgt ccaacttctg
    15421 gtgttttaaa aatcccccca tgccccacta aacgatcaat ttggacattc tcagatttca
    15481 atatatccat tccgattctc attgcaccaa aagcactggc taaatggatc cgcatgaaat
    15541 tcgctaaatc aaaacggcta cctggtgtac gtacaaataa tggccttcct tcatttactc
    15601 ctgtaatgtt ttcaccggaa aaataaccat aagaaagtaa tcccccgcaa tccgtatcgc
    15661 cttctagcgc tttgttgaat aataccgtga ataatttatc tctgcttatc tccataccca
    15721 aagaattggt aaattcttca aaaatactga cccaagcatt gatatccgaa gagcagttat
    15781 tcgtatgaac catgcctact agttctcctg aaggtgtagt gaccatgtcg atttccggat
    15841 ggacattttc tagttctttt tctaatacga tcatagcgaa ggcggaagta ccagccgaaa
    15901 tgttccctgt ccgtttggct acactgttcg tagcgaccat accggttcct gcatcccctt
    15961 ctggcggaca aaccgggatt cctggtctca aatttcccgt agggtcgatc agataagccc
    16021 ctgattcgct taacttgcca gcttcttctc ctgctactag aactttaggc aaaatgtgat
    16081 ccaagtcaat cgctacgtgc gctttttcga ttaactgacc aaaaatattt ttcatttctt
    16141 gatggtaggt tttcgttgaa ggatcgatag gaaacatgcc tgatgcatct cctacgccta
    16201 gtactttctc accagtaagc tgccaatgga cataacctgc aagcgttgtc aaaaaattaa
    16261 tttctttcac atgttcttct ttattcaaaa tagattgata caaatgagcg atactccatc
    16321 tctgcggaat attgtattga aatttttccg ttaatattcg ttctgcttct tcagtgattg
    16381 tatttcgcca agtacgaaat ggtactagta actgttcctt gctatcaaat gctagataac
    16441 cgtgcatcat tgcactgaac ccaatcgacc cgattgtcgt aagattctcg ccatagattt
    16501 cgaatacttc tgctgccatt tttcggtaac tgacgcgcag tcctttccaa atatcttcta
    16561 atgaataggt ccagatacca ttttctagtt tgttttccca ctcataacta ccagcggcaa
    16621 taggagtaaa agatgaatca atcaatacag ctttgatcct agttgatcca aactcaatcc
    16681 ctaaagatgt acgaccatct atgacatcta acatactttg cgcatttact tccttcataa
    16741 tgatttaact ccctccttct cgtcaaacct gatcaaaccg tctttttagt agcgcttaca
    16801 atgattattg tatacttttg tacgtacatg tcaagcggtt atttacaaga atacaataaa
    16861 aaacacttgt tttgtacgta cataagttat aaaatgaaat tgcttacaaa atctgatcaa
    16921 ccgccttctc gaaattctag aaggagagaa tacttatgaa aaaagaaaaa aaagtttcct
    16981 catcgtttat ttattttttt ggcgcatttg gcggcattct atttggatat gatattggtg
    17041 ttatgaccgg cgccttacct tttttacagc atgattgggg tttggcaggt aaagcttctt
    17101 tgataggatg gatcacgtct tcggtcatgt taggtgcgat ccttggcggc tctttatctg
    17161 gtcttctttc tgataagctt ggacgacgaa aaatgattct tctttctgca cttattttta
    17221 tggctggttc tgtcctttct gcatctgccc ctcacaatgg ttcttatttt ctgattgcag
    17281 caaggattct tctaggttta gctgttggcg ctgcatctgc cctagttcct gcttatatgt
    17341 ctgaaatggc tcctgctaga ctaaggggaa gattgtcagg gatcaatcaa gtgatgattg
    17401 cttctggcat gctcctttcc tatgttgcag attatttgct aaaagtgttg cctgaaacga
    17461 tggcttggcg cgtgatgtta gggctcgctg ctgtccccgc gctgatttta tttttcggtg
    17521 ttttagctct accagagtca ccgcgttttc ttatgcaatc tggccgcttg gaagaggcaa
    17581 aaagagtatt gaactatatt cggacaccaa aagaagctga acaggaattc gaacaaatcc
    17641 agctgaatgt caaacaagaa aagacaaccg gaacttcttg gcatactttg tttttagaaa
    17701 aatatcgttc cttagtcatt gccggtattg gtgtagcagt attccagcaa tttcaaggag
    17761 ctaatgctat tttttactat attccgctga tcgtagaaaa agcgactggg catgcagcga
    17821 gcgatgcatt gatgtggccg atcatccaag gaatcatttt agttgctggt tctttgctct
    17881 ttttggttat tgcagacaag ttcaatcggc gaactttatt gaaaataggc ggtagcgtga
    17941 tgggactttc attcatccta ccagctgttt taggaactgt actggatgct catacaaatt
    18001 cgttgttgat tctcttgttt ttatgtattt atgtcgcatt ttattcttgc acatgggctc
    18061 cactgacatg ggtaatcata ggcgaaatat tcccactagc tgtccgcggg cgagcatctg
    18121 gcctagcctc ttcttttaat tggattggtt ctttcctagt tggcttgctt tttcctgtga
    18181 tgactgcttc tatgtctcaa gaaatcgttt tcggcatttt tgggatcatc tgctttttag
    18241 gggttttatt tattcaagag atcgtaccag aaactcgtgg gaaatcttta gaggaaatcg
    18301 aacaaagtgc atctaaaaaa acttatccta aacgtatttc tatccatcat tcttaaagag
    18361 gttgtgacag aagtggtcag cttcaagaaa taagaaggaa ccgcttagtt tccagtcaca
    18421 aggaacaatt tcaaaaaaca gcttctcatg tttgatgaat attgtgactt gtgaccagtt
    18481 ggtaggagaa caccgtttag tggagaggtg gtgaaagaaa cgttcagcct tgagcgatga
    18541 caaaagctct cacaaacatt gcgacgagta accgtaggag caataagagg cggtaaaaac
    18601 ccgtctctta tttttcgttt tctttcactg tttcccgttc aactagtaca ggttcataga
    18661 gaatatttgg ctgtgcctgt cctttttcaa tcgttgtgac gatccaccgt gcggcatctt
    18721 gtcccaactt ttcttttgga tgcgtcaatg ttgtcaattt tttattccct aaatggctta
    18781 aagaagaatc gtctgttcct acgatcgata ggtcatctgg aatatgatag ccattttctg
    18841 tcaacttatc tagcattaaa ctagctattt gatcgttata gcaaatgatg ccatctaaag
    18901 cttgttcttc tttcaaccgt tgtactgttt tttcagcgat tgtttctcgt gtttccgtgg
    18961 tgaaagtaat gatatcttcc gtcaaaaaac gcacattata ctgttcacac gcgctgataa
    19021 aacctttcat cctgtatttt ccttgaagat cgtcgatttt tgtgatgaac aacagacgct
    19081 gatgtccttt ttttatcaaa tattctgctg ccataaaacc gactttcaca tcatctaaac
    19141 aaatataaga ggcatttaac tcttcataag cagcattgat catcaccatc ggaatgcctt
    19201 gttctctcaa agcaacataa agtgctaagt ttgggttata ctgattactt ttcgtaggct
    19261 caacgatcaa accatctacc ccataagcaa tcattttctt caaacactct ttctccaatt
    19321 cgtgatcgtt attcgtactg gctaaaagca atgaataccc agattctcgt aattcatttt
    19381 cgattcctcg gataatcgac ggaaaaatat aatcagagag atatgtggtg atgacaccga
    19441 tcgtttttgt tctgttcaat ggtgtttttt ctttcttata gctgtcatct acatatgtgc
    19501 ccgacccttt ttctttacgc agatagccct catttaccaa cacagcgatt gcttgccgaa
    19561 tcgtatgtct gctgacgtca tatatttctt gcagcttgaa ttctgaggga atggttgtat
    19621 ttacttcgta ttcctcacga aggatctttt gttttatctc ttctgcaata tcttgatatt
    19681 ttgccatttg ccttgcccct ttttatttgt acgtacatat atcataacga gtctgcaagg
    19741 tttattcaag caaaaatcaa ggtttcttgt gctcataagg catacggaac aggttcacca
    19801 aattctggaa atccttgctc attccacgta aagcattgcg cgttcgcgtg ccgattagga
    19861 ttgtctaacg gatccccttt ttggttcttc tctggtcgag catgatagat caaaatatca
    19921 aagcacccgt cttctgaaac cgtaaacgag ttgtgaccag gaccaaattg cttatttttt
    19981 tcagaagaaa cgaatactgg ctccgataat ttatgccatg attcttttaa taaaagattc
    20041 ttgtgctcat cagcccacag caggcccatg gcatagtttt catctgttgc actaccagaa
    20101 tacgtaatga atattttccc attgcgagta aggactgctg gaccttcatt tactgaaaaa
    20161 ccccgttttt cccattcata ctcaggtata gaaaggagga tctgcttccc ctttaacgtc
    20221 caaggattat ccatttcgga tagataaaga ttagaattcc cagtaatttc aggatcttgt
    20281 tgggcccaca cataatatag ctttccttga gaagagaaag ttgttccgtc taaactgaaa
    20341 gtttcccttt tcgtttttat ttgtcccttt tctatccaat ctgtattcat tggatctata
    20401 tgctgatttt caagtacgaa catgcggtga tgatgagatt catcacgtat agttttatga
    20461 ttgcttgcag caaagtaaat ataccatttt ccattgatat aatggacttc aggtgcccag
    20521 atcaattcac tcatcggtcc ttcttcatga gcctgccata caatcaggct tgcagcatgt
    20581 tctaattcat tcagtgactt tgcccttctt aattcaatca cttggtagcc tggaaccgat
    20641 cctgtaaaat aataataacc atctgtatgt ttatataccc aaggatctgc ccgttgccga
    20701 acgataggat tattataaag tgtcttcatc ataacaaatt tcctcacatt ttctttattc
    20761 taattacatt ccaagagtat ttttctatcc ctacatttaa ttcgtcatgt ttttctattg
    20821 aaaaatagtt tatttcaggt tttatttttt ctggttcttc acgtgtattt ttgtccaaca
    20881 gatcgctact ggttaatgtg tagtgctctc cttgatctag caaattgaaa tcatgttcaa
    20941 tcgttatctt ttgttgttca tttgtgcgat tgatcaagaa aatagtcagc tgattgccgt
    21001 tatctacgat cacactgtcc acataaggaa cattttttat cttttcacaa tcataaacag
    21061 ggcaaatgat ttgttgtttc aatacttgac ctttagcata tttcgataag tgttgcatca
    21121 cataataagt tggattcctc catgcttgtc ctttagcatc tgtcagtatt aaaggtattg
    21181 tgtttactaa caatgattga caactaatct ttacgcgatc tgcgtgtcgt aaaatagcca
    21241 gccctaatga accaaataat aatgtatctt ccatagtaaa gtaacaccaa tcgatgggac
    21301 taccgacttc aaaatcccta tgtctttttt ccggttgggc atgtacattc cattcatcaa
    21361 acgctagata cattgttttc tcacttctca aatatccttt tacataatca caagtagcaa
    21421 cgatttcttc aatttgacga tcgaatcgat aagcttttgc aaggtatgtc gctgtatcat
    21481 cacgctcatt tttaggaggt ttagtcaagt cttcttcatc atacttatca atatagttat
    21541 gcaaagctaa ataatctaca ttctcataag cttcttctaa tgcgatacga tcccattcag
    21601 ggtacgatgg taaacgtggc gtagagcttc caactaagat tgtttcaatt gaatcatcca
    21661 ctaacttcat gacttttgag gcttcatttg ctaacctacc atattcataa gcactcttag
    21721 cccccacttg ccatggaccg tctagctcat tccctaaaca ccataattta acgccaaaag
    21781 gctttttttg cccatttttt attcttaaat cactccagaa ggttcctttt ggaaaattac
    21841 aatattctac taaatttttt gcttcatcaa ttccttttgt tcctaaattc accgtgaata
    21901 tcggtttcgc ttgtacatga ctgatccatt taaaaaattc gtggagtcca aaagaatttg
    21961 gttctatacc tcgccatgct aaatcaattt tttttggtcg ttgttctttc ctacctacag
    22021 tatccaccca atcataacca gatgtaaagt ttcctccagg atagcgaatg acacctaatt
    22081 ctaattcctt tacataagag aggacatctt gtctaaaccc gtcaccatca gctgtttcat
    22141 ggcccggttc ataaatccct gtatagacga cacttcccat atgctcaaca aatgagccaa
    22201 acagccgaga actgatctct cctaccgtaa aatcctctct gatttttact ttcacttctt
    22261 cgtccaacag ctttccctac ctttcatttc ctatagtcaa gataatagat acaagtttcc
    22321 atacgccacc agcaaagata aaaataaaag gagggaaaag aaatatgagc agtacaaatc
    22381 ctaataacaa tacgcaacaa agaatatttc tcaaaaaatc aaaaaatact ttccatgcac
    22441 tttgttcaaa aatttgtaaa aatgtgatat ctttgttttc agaaatcaca tatatccagg
    22501 ataaaagaaa catggaataa ataagtaaaa ctacatatcc actaattgaa agtacgattg
    22561 tctgttcttc taataattga ttaacaagaa aataaataat gatcgttaac agactaagga
    22621 acaagttctt accaaaatgt tgctttattc cttgaaagaa aaaataaatg atatttcctg
    22681 atccctcacc tttccattgc gccattacat aaaaaacagc acatgtagca ggcacgatag
    22741 taataacagg caaacacacg attacccaga gaatacctaa taagatgtaa tcagaaacca
    22801 caatcaactg tccataaaat ttagtctcaa ctattttcat cttcctcact ctttcattcc
    22861 agacatagct gttccttgaa caaaatattt ctgtgcaaaa atataaacca ctagtaatgg
    22921 cagcattgaa ataaccgttc cagccattac aggtccataa aaagaaatgt tctgccctgt
    22981 aaataaagct aaccctgctg gtaaagttct catttttgaa ctacttgtca atatcaatgg
    23041 ataaagcaag tcattccaac tattcatcag cgtgaaaatc cctaatgata aaagagtagg
    23101 tttgctcaat ggcaacatga ttttcaaaaa aatattaaac tcacttaatc catcaatgcg
    23161 tgctgcctct tccaaagaat ctggtaaagt aataaaaaag gaacgcatca tgaagatacc
    23221 aaatgcagtt gtcatacgag gaatgatcaa acctgcataa gtatcaagca tgccaagcaa
    23281 attgacttca ataaatagag gaatcatgaa gacttggaat gggatcatca ttgtcagcaa
    23341 aacgaaatag aacaaaactg attttccttt gaaacgcata cgagcaaatg cataccccgc
    23401 aagagaatca aagaatacag aagtgatcac tgtccctcca gcaaaaatca ctgtattttt
    23461 gatgtaatct aataaaggga tcgttgtcca aaccttaata tagttatcta gggtatagct
    23521 atctgcaaat attctaggcg gaaatgagat aatgtctttt tcgtgtttaa aagaagaaat
    23581 aatcagccac aaaaaaggaa acgcaataac caacaatata actagcatac taatcgttcg
    23641 aatgattttt ttaccactaa ctgcttttcg actattcaca gtaactgtaa tcgagctttc
    23701 tggtgccata caggtttccc tcctatttca tttgtttttc tttccgtgac atgtacacat
    23761 tagtggagat agctatagtt gcaacaatga taaacaaaac cgaagacagt gctgatgcat
    23821 agcccaattc ataaggtgct gtaaatcctc gactgtaaat atattgaaca gctgtttctg
    23881 tcttaaattg tgggccacca gcggttgcaa catatacttg gtcaaacact tgcatcgaac
    23941 caatcagatt cgttaaaata caaaaaccta aggagggaac gatctgaggt agcgtaatgt
    24001 taaaaaactg ctgtcgattt gttgcaccat ccatttgagc agattcatac aaagagggag
    24061 agattccctg aatagcagtc aagagaatcg tcatagtaac accaaaattc ttccaaacag
    24121 tcatgatggc taccgtcggc atagcaaggg ttggatcgcg aaaaaattga gggatcggca
    24181 agccgacttg tgaaaacaag taaggaatcg cccctacatt cggatcaagc aacatagaaa
    24241 aaataatcgc aattgacgtc aatgaacaaa taactggaat gtaaaatgtc gaacgacaaa
    24301 aacgattgaa tctggtattt ttagataatg ctactgctgc taataaacca acgataattt
    24361 gtgttggcgt ttcgagtacg gtgaaatata aagaatgaat caacgaatta actgctcgtt
    24421 catcactaaa tatacgtgtg aaattttcaa aacttacaaa atttcttgat gtaaagaaaa
    24481 tattcaaatc tgtaaaacta atagcaaatg ttcctaaaag cggtatgaca gtaaataata
    24541 ataaaactaa aaaagatgga gttaaaaaca aatacgcagc acgatcagga cgattccaat
    24601 atttggtgaa tccgttcata tagtccacct catctttcat tttgataaaa tatacaatct
    24661 tggtttaaac gaatctgaat aagatatccg aacaatcgct gtgatttttg caataacacc
    24721 tatctgcaaa tactaggtcg atcattcaga tatctgtatc ttgccaacag tctatcctgt
    24781 ataaagttct gtactatttt tgcagtaatt gattgatggc atcatctgca tcgttcatga
    24841 gtgtctcagg atcttctcct gctaataatt tttcaatcag tggattgatg atatcgttat
    24901 tgatcgcaga gatttgggta aaacctggta aatatggctc tgcatagtcc atttgtttcg
    24961 ataattcaga tacaatagga tcatttttta cttcttcatc ttttgctact gaatccaagt
    25021 aggcaggaaa accattttcg atactccatt ttttaccaat ttcagttgta ttccaatatt
    25081 tcaaaaactc atagacagct tcttttttat tattatctgt acttgccgga atggcatatc
    25141 ctacgccatc taaaatagct gtttttttgt ctgaatcact cccttgtggt aaggtagtca
    25201 cattataatc aatctcgttt gctttcaatc cgttatttaa ccatggccca ttgatttcaa
    25261 tggctagttg accagcattc attaaattgt ccatttcggc gccagaaatc gattctggac
    25321 cagtcttgtc tacatggatc attttttgga tcgcttttaa cgttttaagg ttctcaggag
    25381 acgcaaattc agacttagta taatcatcgt tcactaattt accgccattt gctaacatcc
    25441 agttatataa cggtgcagtt ccatccttat tgaagacaaa acctgataca tttttactac
    25501 tatcagttaa ttttggtgcc atttctgtca attcttccca agtagtaggt ggttgttcag
    25561 tatctaatcc agcttgttta aacaaatcat tattccaata catatacatg ccttgtacct
    25621 gcatggggac aaaatactgt ttaccttcta cttcacctaa ttcaatagcc gtttctttaa
    25681 aatcagattt atctactcca tcatattccc agaaatcgtc taatggttgt accgctccgt
    25741 tttctacata ctgtgcaaaa tctggataat ttaatgcaat aaagtcaggt gcttttttag
    25801 aagaaatcgc tgtaggaagt ttttcattca aattggccca agtcatgata tccatttcaa
    25861 ttttgatgtt tttatcattt gttttattaa attcattaac gatttcttgc aatatttcac
    25921 catcagatgc agtaaaccca ttccaaaaag tcaaagtcac ttctttatca gaatcagatt
    25981 ctgctgaact tccgccaccg caagctccta aaatacctgt tgcagctacc aacaaagagc
    26041 caattaacca ttttttacct ttcatttttt gttcctcctt catctgataa acgacatctg
    26101 accacattcc aagatagcgg tttcaataaa caattgattt tcccatcttc agcttttaca
    26161 tttgttgaat gacttggttt tacttgttca cttttaggaa gattagttgc ttttgtatca
    26221 aatccactca tttcagaaaa atcaatcact ccctctaatg ataattcaga caagtgaaca
    26281 gttagattca tttcttcttc tccacggtta actgcaaata tgacaagttc tttgtcattt
    26341 attacggcta tactttctaa atacggcact tcttcaaaat cttccgtcgc atagtttgct
    26401 gaacgaattt gtgggcttaa cacctctcct tgtccataca cagcagcttg cataaatgga
    26461 taatagatcg tctgtctcca agcttctccg tttgtttctg tcctgattgg tgcaataacg
    26521 tttaccaatt gagcaagaca agcaatcttt actctatctg catgtttcaa taaagtgatt
    26581 aatagacagc ctaatagtaa tgcatcttca aaattatagt tatcttctag taaaggagga
    26641 gcaatctgcc aaggcgctgc ttgctcatct tgttccgctg aatgatacca aatattccat
    26701 tcatcaaagg acaggttgat ctgcttctta ctatgtttct tcgccttgac tgtatcgcac
    26761 atcgctgtca cttcagaaat gaaccgatcc atgtctaagg aacgtgcaag ataatgagaa
    26821 agatcatttt tttggttgcc ataatattga tgcagagaca aataatctat ttgatcgtac
    26881 gcttgatcaa gaactgttaa ctcccaatca ccaaatgtag gcatttgccg attcgaactt
    26941 ccgcataaaa ccacctcaat tgaatcatct acgagtttca tcgccttagc cgtttcctct
    27001 gctaaccgtc catattcttc cgctgtttta tgtccaatct gccaaggacc gtccatttca
    27061 ttccctaaac accatgtctt aattccaaaa gggctctctt gaccattcga acgccttaaa
    27121 tcactccaat aagttccttt aggaaaatta caatattcga ctaaattacg agctgcatct
    27181 actccacgag tacccagatt gacagccata ttgatttcgg cccccacatg ctgacaccag
    27241 ttataaaatt catgaatccc aacttcattc gtttcaattg tccgccaagc taaatccaac
    27301 cttctaggtc tatttgctat gggcccaata ctgtcttccc aacgaaatcc tgacacaaaa
    27361 tttccaccag gatatctaat catgggaatg ttcaattctt ttaccaagtg aatgacgtct
    27421 tggcgaaatc cacttttatt agctgatgga tgatctggct gataaatccc ttcatatact
    27481 gctcgtccca tatgttcaat aaatgaactg tacaaccgcg gatcaacttt gcctacacgc
    27541 ttgacactac taatcaacat tgtggcttcc ataactgacc tcctttaatt tatacatgaa
    27601 taatacacct cgttgaaagc gttgtcaata atataaatgt taaacttact tattttttat
    27661 cgattttaat taaccaaaat attaataatg attaaaacaa ataatttaat ataactttta
    27721 ttaacaaata tgttaatata taagaaaaac cttttaagag gtgatatata tgcaactaca
    27781 aataaacgaa gaatccttac cggtatatga agcattagct agtaaaacaa ggatcaagat
    27841 catccagcta ctttccaaaa aaaagatgaa tgtcaaagat ttggcaaaag aacttggagt
    27901 gagcagtgct atcacgacga tgcacgtgaa aaagctagag gaagcaaaca tcatcaaaac
    27961 agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc gataaaatcg atattagttt
    28021 tccagaaaaa atattcaatg cttttgatac gaaagaaaca tcgataccta ttggtcacta
    28081 tacaaattat gcaattgaac caacatgcgg tttagctacg atccatgatt ttatcgggaa
    28141 agtggatgaa ccacgctatt ttatggatcc tcgaaggatg gatgcccgca ttctctggtt
    28201 taccagcgga tttgtcgagt atcaggcacc taatttttta aatacagagg atacattgga
    28261 gatgttggaa gtttctgtgg aaatttcttc tgaatttcct ttttcaaatg acaattggcc
    28321 ttcagatatc actttcagcc taaatggtgt ggagctagga acatggacga gccctggaga
    28381 ttttgctgat atccgcggta aatatacgcc tgattggtat ccagataatc ttaatcaata
    28441 cggcttgctg aagacgatcc gaattaccaa acacttgacg aatatgaatg gagaaccctt
    28501 gtcaaacatc acgatcaacg acatccctaa agagcaagac acttggcatt tgcgaatcga
    28561 agtgaaagac gatgccaaac atgtgggggg ctgtacttta tttggtaaag gattcggtaa
    28621 ttacgatcag gatatcaaat taaaagttta ctacagctga tatttttctt ttctatttta
    28681 taaaacaaaa acagccaatc cctatgaagg gacggctgtt tttgctttat tgttatttcc
    28741 agaaatcatc caaaacggaa attggcaaat gtcgtttatg ttgtgtcttg ttccaccatg
    28801 cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc ttctagataa tcatcaattt
    28861 cattatatgt gacacctagt gcgacttcat ctgcaatcat tggtttccca tcttcaagat
    28921 ctgcagtcgg aatttttgtg tataattttt ccggtgcatt caatgcttga agcaattgtt
    28981 tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc ggctccacca tcaccaaatt
    29041 tcgtgaagaa tccagtaatg ttttctgcag catgatctgt ccctaatacg gctcctgctc
    29101 tttctccagc tacggcatat tgcgtaatca tacgttgacg tgctttgata tttcctttgt
    29161 taaaatcgct gattcccaca cctgcttcgc ttaatacttg tacttgtgca tcaacagctg
    29221 gtttgatatt tactcgcaga ctaacatcgg gctggataaa gtcaagagca gcttttgcat
    29281 cttcttcatc tgcttgttca ccataaggaa gacgaacagc gataaattga tagttttcat
    29341 cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc caaacggcct gctaaagtag
    29401 aatcttggcc gccgctgata cctagtacaa aagttttaag aaacggatat tttttcaaat
    29461 atgctttcat aaagtcaata cttttacgta tttcttcttt tggatcaatt gttggtttga
    29521 cacctaattc attgataatt tctgtctgca ggctcattta ttctcctcct atgcttctgt
    29581 atgaagttct gctacgcttt ttttcattcg ttccatgata gccattttat ggttccagca
    29641 ttcagtagac agatcgactg ggtatttttg cggattcaag tctcgtttat actcttccca
    29701 tagtgaatct agattttctt ttgtatattc tttgatttcg tttagtgcag gtaattcgta
    29761 aactcttttt ccttcgataa agatatcttg taaaactggg cgagctgtaa agtctcgaac
    29821 agttttattg ataaacgtat gcactggatg gaacatgaag atagcttctt cttctcgagg
    29881 gtcttcatcc cataatgtca cgtagtctcc ttcagatttc ccatcaaaat ttcgagtgat
    29941 gcgccacact tgttttttcc ctggagtagt tactttttct gcattgcttg ataacttgat
    30001 cgtatccatc atttttcctt catcatcttc gatcgatact agcttgaata ctgcaccaag
    30061 ggctggctga tcataggctg tgatcaattt tgtcccaaca ccccatacat caattttagc
    30121 tttttgcatc tttaaattca aaatcgttgc ttcatccaag tcattcgaag catagatttt
    30181 tgcttccgta aatccggctg catctaattg ttcccgaaca cgcttagata tataggccat
    30241 atctccactg tcgatgcgta cgccttggaa gttgattttg tcacctaatt ctttcgccac
    30301 gcgaatcgca cttggcacac ctgatttcaa cgtatcataa gtgtcgacta agaacacgca
    30361 gtctttatgg gttttagcgt aggccataaa tgcttcataa tcatttccat aagattggac
    30421 taacgaatga gcatgggtcc cgctagctgg tatcccaaat atcttcccag cacgtacatt
    30481 gcttgttgca tctgctccgc cgatatatgc cgctctagtt ccccagactg ctgcatccag
    30541 ttcttgtgcc cgtctagttc caaattccaa tagtggatct tctccgatga cagatttgat
    30601 acgagctgct tttgtcgcga tcaacgtttg gaaattgacc atattcaaca gtgctgtttc
    30661 tactaattgg cagtgcgcta aaggcccttc cacttggatc aatggttcat ttgcaaaaac
    30721 tagttctcct tcacgagcag aacgaactgt tgctttaaat tcaaaatttt gtaaatactc
    30781 caaaaaacct tcaggataga cttctaatcc acgcaagtaa gcaatatcgg aatcattgaa
    30841 cgtcagattc tctaaataat tgactaaccg ttccaatcct gcaaaaaccg cgtatccgtg
    30901 gttaaaaggc atttctcgaa agtaacattc aaaaacagca tgtaaatcag ctcgacctaa
    30961 ttcccagtat gtctgcatca tattgatttg atatagatcc gtatgcagcg tcaaactgtc
    31021 gtctgggtaa gtattattca tcttgtatta ctcctcttgg caagttttca tttcctaccc
    31081 attataacaa aaaaaattaa taggaaaaga aatcttccat tttttctgaa aaagcacatg
    31141 ttttacggat atttcttgct aaaaatgaac tccatctgct aaaactatcc tttcttattg
    31201 gtatttaacc taaacgagaa agaatactta ttttattcaa tgtatccgag acacgaagaa
    31261 attagcgttt tctatttttc ttacttgata aaataagtag attttcctac tacaatcgta
    31321 gccgttaaga taccgatgat tgaacaaata taaccaaatg tctctacaac ataatcaatg
    31381 gcaaaagttt ttgaagcaat aatacgtctt tccttttttg cttcttatcc ccaaagaaac
    31441 ggatataact tattatgtat tgaatttttt tggaaaggcg aattatgaca agatatacct
    31501 tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt aaaaagatac aaagtaagaa
    31561 agcagctgtc aatagaatgg aaacaaaaat ggcgagacat caatcagtct cgccttgttt
    31621 tt
//
LOCUS       DAZKBH010000019        30589 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000019 DAZKBH010000000
VERSION     DAZKBH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.019"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            363..731
                     /locus_tag="ACG8OO_000891"
     CDS             363..731
                     /locus_tag="ACG8OO_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9044815.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="ACG8OO_000892"
     CDS             911..1852
                     /locus_tag="ACG8OO_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9044816.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="ACG8OO_000893"
     CDS             1921..2652
                     /locus_tag="ACG8OO_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9044817.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="ACG8OO_000894"
     CDS             2796..4976
                     /locus_tag="ACG8OO_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9044818.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="ACG8OO_000895"
     CDS             4996..5478
                     /locus_tag="ACG8OO_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFP9044819.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="ACG8OO_000896"
     CDS             5494..6255
                     /locus_tag="ACG8OO_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9044820.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /gene="murC"
                     /locus_tag="ACG8OO_000897"
     CDS             6386..7720
                     /gene="murC"
                     /locus_tag="ACG8OO_000897"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9044821.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="ACG8OO_000898"
     CDS             8027..8512
                     /locus_tag="ACG8OO_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFP9044822.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="ACG8OO_000899"
     CDS             8505..9188
                     /locus_tag="ACG8OO_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9044823.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="ACG8OO_000900"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="ACG8OO_000900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9044824.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="ACG8OO_000901"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="ACG8OO_000901"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFP9044825.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /gene="coaE"
                     /locus_tag="ACG8OO_000902"
     CDS             12804..13433
                     /gene="coaE"
                     /locus_tag="ACG8OO_000902"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9044826.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="ACG8OO_000903"
     CDS             13485..13817
                     /locus_tag="ACG8OO_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9044827.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="ACG8OO_000904"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="ACG8OO_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9044828.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="ACG8OO_000905"
     CDS             14643..16016
                     /locus_tag="ACG8OO_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9044829.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="ACG8OO_000906"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="ACG8OO_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFP9044830.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="ACG8OO_000907"
     CDS             17071..17511
                     /locus_tag="ACG8OO_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9044831.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="ACG8OO_000908"
     CDS             17655..18356
                     /locus_tag="ACG8OO_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFP9044832.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="ACG8OO_000909"
     CDS             18353..19474
                     /locus_tag="ACG8OO_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFP9044833.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="ACG8OO_000910"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="ACG8OO_000910"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9044834.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="ACG8OO_000911"
     CDS             20853..21761
                     /locus_tag="ACG8OO_000911"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9044835.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="ACG8OO_000912"
     CDS             21796..22068
                     /locus_tag="ACG8OO_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFP9044836.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="ACG8OO_000913"
     CDS             22079..23125
                     /locus_tag="ACG8OO_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFP9044837.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="ACG8OO_000914"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="ACG8OO_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9044838.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26822)
                     /locus_tag="ACG8OO_000915"
     CDS             complement(26136..26822)
                     /locus_tag="ACG8OO_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044839.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26800..27294)
                     /locus_tag="ACG8OO_000916"
     CDS             complement(26800..27294)
                     /locus_tag="ACG8OO_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9044840.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="ACG8OO_000917"
     CDS             complement(27314..28534)
                     /locus_tag="ACG8OO_000917"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9044841.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="ACG8OO_000918"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="ACG8OO_000918"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9044842.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        10250 a         5445 c         6593 g         8301 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataatattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30541 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtg
//
LOCUS       DAZKBH010000020        30104 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000020 DAZKBH010000000
VERSION     DAZKBH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.020"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(335..2062)
                     /locus_tag="ACG8OO_000919"
     CDS             complement(335..2062)
                     /locus_tag="ACG8OO_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFP9044843.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2055..3248)
                     /locus_tag="ACG8OO_000920"
     CDS             complement(2055..3248)
                     /locus_tag="ACG8OO_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9044844.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3435..4079)
                     /locus_tag="ACG8OO_000921"
     CDS             complement(3435..4079)
                     /locus_tag="ACG8OO_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9044845.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4173..4307)
                     /locus_tag="ACG8OO_000922"
     CDS             complement(4173..4307)
                     /locus_tag="ACG8OO_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFP9044846.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4309..6441)
                     /gene="feoB"
                     /locus_tag="ACG8OO_000923"
     CDS             complement(4309..6441)
                     /gene="feoB"
                     /locus_tag="ACG8OO_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFP9044847.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6438..6911)
                     /locus_tag="ACG8OO_000924"
     CDS             complement(6438..6911)
                     /locus_tag="ACG8OO_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFP9044848.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7312..7536
                     /gene="nrdH"
                     /locus_tag="ACG8OO_000925"
     CDS             7312..7536
                     /gene="nrdH"
                     /locus_tag="ACG8OO_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9044849.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7695..9854
                     /gene="nrdE"
                     /locus_tag="ACG8OO_000926"
     CDS             7695..9854
                     /gene="nrdE"
                     /locus_tag="ACG8OO_000926"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9044850.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9904..10869
                     /gene="nrdF"
                     /locus_tag="ACG8OO_000927"
     CDS             9904..10869
                     /gene="nrdF"
                     /locus_tag="ACG8OO_000927"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9044851.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10958..12097)
                     /locus_tag="ACG8OO_000928"
     CDS             complement(10958..12097)
                     /locus_tag="ACG8OO_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9044852.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12406..13119)
                     /locus_tag="ACG8OO_000929"
     CDS             complement(12406..13119)
                     /locus_tag="ACG8OO_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9044853.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13373..14074)
                     /gene="nagB"
                     /locus_tag="ACG8OO_000930"
     CDS             complement(13373..14074)
                     /gene="nagB"
                     /locus_tag="ACG8OO_000930"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9044854.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14308..15840
                     /locus_tag="ACG8OO_000931"
     CDS             14308..15840
                     /locus_tag="ACG8OO_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9044855.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16031..17233)
                     /locus_tag="ACG8OO_000932"
     CDS             complement(16031..17233)
                     /locus_tag="ACG8OO_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9044856.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17315..18004)
                     /locus_tag="ACG8OO_000933"
     CDS             complement(17315..18004)
                     /locus_tag="ACG8OO_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9044857.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18110..18478)
                     /gene="rplL"
                     /locus_tag="ACG8OO_000934"
     CDS             complement(18110..18478)
                     /gene="rplL"
                     /locus_tag="ACG8OO_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9044858.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18531..19034)
                     /gene="rplJ"
                     /locus_tag="ACG8OO_000935"
     CDS             complement(18531..19034)
                     /gene="rplJ"
                     /locus_tag="ACG8OO_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9044859.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19238..19365)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19444..20133)
                     /gene="rplA"
                     /locus_tag="ACG8OO_000936"
     CDS             complement(19444..20133)
                     /gene="rplA"
                     /locus_tag="ACG8OO_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9044860.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20237..20659)
                     /gene="rplK"
                     /locus_tag="ACG8OO_000937"
     CDS             complement(20237..20659)
                     /gene="rplK"
                     /locus_tag="ACG8OO_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9044861.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21002..21670
                     /gene="sdaAB"
                     /locus_tag="ACG8OO_000938"
     CDS             21002..21670
                     /gene="sdaAB"
                     /locus_tag="ACG8OO_000938"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFP9044862.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21683..22555
                     /gene="sdaAA"
                     /locus_tag="ACG8OO_000939"
     CDS             21683..22555
                     /gene="sdaAA"
                     /locus_tag="ACG8OO_000939"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFP9044863.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22646..23746)
                     /locus_tag="ACG8OO_000940"
                     /pseudo
     CDS             complement(22646..23746)
                     /locus_tag="ACG8OO_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(23838..24725)
                     /locus_tag="ACG8OO_000941"
     CDS             complement(23838..24725)
                     /locus_tag="ACG8OO_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9044864.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(24802..25347)
                     /gene="nusG"
                     /locus_tag="ACG8OO_000942"
     CDS             complement(24802..25347)
                     /gene="nusG"
                     /locus_tag="ACG8OO_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9044865.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25448..25618)
                     /gene="secE"
                     /locus_tag="ACG8OO_000943"
     CDS             complement(25448..25618)
                     /gene="secE"
                     /locus_tag="ACG8OO_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9044866.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25642..25794)
                     /gene="rpmG"
                     /locus_tag="ACG8OO_000944"
     CDS             complement(25642..25794)
                     /gene="rpmG"
                     /locus_tag="ACG8OO_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9044867.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25956..26600
                     /gene="cbpA"
                     /locus_tag="ACG8OO_000945"
     CDS             25956..26600
                     /gene="cbpA"
                     /locus_tag="ACG8OO_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFP9044868.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26656..27159)
                     /locus_tag="ACG8OO_000946"
     CDS             complement(26656..27159)
                     /locus_tag="ACG8OO_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9044869.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27172..27218)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27756..28721
                     /locus_tag="ACG8OO_000947"
     CDS             27756..28721
                     /locus_tag="ACG8OO_000947"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9044870.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28820..29992)
                     /locus_tag="ACG8OO_000948"
     CDS             complement(28820..29992)
                     /locus_tag="ACG8OO_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9044871.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29979..>30104)
                     /locus_tag="ACG8OO_000949"
     CDS             complement(29979..>30104)
                     /locus_tag="ACG8OO_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9044872.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9178 a         6190 c         5364 g         9372 t
ORIGIN      
        1 aaaaaacaat tccttcttga aacgtagcaa aataattcct gaataagaat ttaatcactt
       61 ttaagtatat aaactatgta tattctttaa tattttttga gaatgcactt cgtacataat
      121 aaactttaat cacattagtc tttcatacct acgatcgatc tctttgactc gcacccataa
      181 attcataata gtttgtcggc gtctttggac gtttcgaaga tgagctacgc attctctttt
      241 tttatagaca aaaaagatag agacgaacat ggagcttcca aatttggata tttgatccaa
      301 ttttggaagc tccatttatt tcgtgtctgt ctttttattt tttccagctt tcgactaatt
      361 cttggttctc tttgatccag tcttttgctg cttgctgtgg atcttttcca ttgttgattt
      421 ccaacataac tttttccata tctttttctg accaatggaa attgtctaat actttgtaag
      481 cttctggctg gtcttctttc aatccttttc gaaccatcgt atggatctgc tcttcgcttc
      541 ccattccgtt ttcaggatct tctaagtatt tcagatcata tttggcaaac atccaatgag
      601 gtgtccatcc tgtaactaca attggttcgt gttttttgat cgcttgacct agtgcaacgg
      661 tcattgctcc tgaagaagat gtctctactt tccagtcttt caagttgtca tatttttgga
      721 tcgtgttttc agcagccgta actacaccag ctcctggctc gattcctatg attttcttac
      781 ctgcctgatc ggttaactca tcgatcgaat ttacatccat gtaggctggc actgctaaac
      841 caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac ttgatcttta tattgtgcat
      901 attgtgaacc atgagtttta ggtagccaag ctgaaaccat ggcatctgac tcgccatttg
      961 aaacagattt ccacatgatt gaattatcta atggcgtcat cgttacatca tagcccattt
     1021 gtttcaatac ttcaccgact acatttgttg aagcaacttc tgtatcccat tcaacataag
     1081 ataagttgat acgttttgtt tctttggcag atgaaaaact ccctatacca atcgcaccta
     1141 agatcactac tactacagca ccaatgatca gtccttgacg ttttttactt ttctgtgttg
     1201 ttgctcctgc agctttttta ttgtttggct ggttcaaatg ctgtgtaaaa cggtcaatga
     1261 tgattgctaa gataactaat gcaaccccgt tgacaaatcc attcccgact tgtgctcttt
     1321 gcaatgctga taatacgccg cgtcctaagc cgggtgcacc gatcatagag gcaataacga
     1381 ccattgaaag tgacagcatt gttgtttgat tgatccctgc catgatcgta cttttagcta
     1441 aaggcaattc tagtttgaat agtttttgac gacttgtgct cccgaatgaa tcagaagctt
     1501 ctactaattc tttaggaact tgtcgaatcc ctaaattcgt aaaacgaacg gttggtggca
     1561 gagcaaagat gactgaggca aatacccctg gaaccatccc gataccgaaa aatgcaactg
     1621 ctggaatcaa ataaacgaat cctggcattg tctgcataaa gtcaaggatc ggtttgataa
     1681 tactttgggc tttactgctt tttgccatca agatccctaa tggtacccca atgatgatcg
     1741 agatcacact agataaaaga acaagtgtga cagtattcat caaatcattc catagacctt
     1801 ggttataaat aaaccataga ccgattaatg tgaacaacgt aagtcctgga cgtttcttag
     1861 aaataaagaa agctgctacc gtcaataaca agataaacaa aggagctgga atcactaaca
     1921 gtaaatttgt aatccccgac atcactgcct gtccaatcgt ttgtaaaaat gaaaataggc
     1981 cagaaaaagt tgtcgtaaac cattctgtga tattttctac ccagtttgcc actggtaatt
     2041 gatagttcaa tagattattc atgttcactc acctcgcttg tttctgctaa tgcttcaatc
     2101 acactacctc ggatgacgac accttttaat tttccatcct ctacaactgc aagtggagtc
     2161 ggtgagtcat aaataatatt gaaaatatca cttaccacca tgtctttccc aatcaccgtc
     2221 acattttcat ctaggaagtc gatcaatgga cgtttttgct tcctagcttc taacgcttga
     2281 tctgcagtga tgatcccttt caggtgtctc tttctgtcaa cagccatcag catgctgact
     2341 tcttcattac gcatccttgt caatgcaacg tttggtccat cagattcgat attcgtcgtc
     2401 aatgcaggta ccatgatgtt ttgagccgta agcactttgg aacgatcgac ttcttccaca
     2461 aattcacgta cataatcatt tgctggattg gtcaatattt cttcacctgt tccgatctgc
     2521 atgatttcgc cgtctttcat caaggcgatt ctgtccccaa tccgtaaagc ttcattcaaa
     2581 tcatgggtaa tgaaaataat cgttttttga acattggctt gcaggtccag taattcgtct
     2641 tgcatttctc gccggatcag cggatcaaga gccgagaacg cttcgtccat cagcaagatc
     2701 tcaggatcat ttgctaaagc tctggctagg ccaacccgtt gttgcatccc accagataac
     2761 tgactaggat attggtcttt aaatgaaagc aaactagaat tttctaatgc tttttctgct
     2821 ttttcttgtc gttcttcttt aggtacacca cggacttcta acccgtattc tgtattttcc
     2881 aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa ccatattgat tttatgacga
     2941 cggacttctc gcaattcttc tttgcttaac ttcgatacat cttctccatc aatatagata
     3001 ctgcccgaag ttggctcaat cagacgattc aatagacgga tcaatgtgga tttcccactc
     3061 ccggaaagcc ccatgatcac gaagatttct ccttctttta cgtcaaaatt cacgtcatac
     3121 acaccaaccg ttgcaccggt tttctttaaa atctccgttt tcggctggtg atcattcatc
     3181 atatctaaag cggcttgtgt ctttttacca aatatctttg ttaaatgttt tacttctact
     3241 tttaccaaac aaattctcct tttttatgta aattcatttt tctttctgaa aaaagtgacc
     3301 acactatgtt aacatcatgt agtcactttg tcaaaaatga aagaaatttg cttgtccaaa
     3361 ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt agccaagtta atattttttt
     3421 aatatttcac acaatcattc tgcaagctga tgctccgggt aaaaaaagtt tgtcatacgc
     3481 tgtagtgtat attcatttcc aataaaatga acgatgtccc ctgccgtaaa tttagcatag
     3541 gggccaggtg aaacaagcag ttcttcatta tgagaaattg cgataactgt ggcgccagtc
     3601 gcctgccaga tattcaactc gctaattgaa cggtccagat gcattgcttt ttctgataaa
     3661 gataattcaa acggaagtaa aggattcatc ttatgtattc gcttcgtttg attgaccagc
     3721 tgatccaata attgagaaaa atgattcagc tcatcttgtt gcttctccac acttgccatg
     3781 atttctgctt ttgtatcttg gatggattgt acatcttgat attgcaatag gaaatctttt
     3841 gctttttcct tcgaagcaac aaatgcccca cttccatgcc gtacttccat gattccaaga
     3901 tctaccagca cattgatagc cttgcgggta gtctcaggag aagcattgta cgtactggca
     3961 agtgttgagc gggcatggat cttctcaccc actttatatc ggttttcaac gatacgttcg
     4021 gctaaatcaa cagcaatctg ctggtagcgc gggcggacga tttttttctt tccagacatt
     4081 ttctttcctc tttctttttt tactttccct catcttacta tgctctagca aaataagcaa
     4141 aaaaggggag aatagcctct tgcaacgact gatcatcgct tgatctgttc cttttttact
     4201 gggcaagctg tttgacagtc atcacaattc ttaccggatt ttaaacgagt atagacaatt
     4261 gcgcctgcac ttccaaagat taacacactt aataagaatg tcgccatcct attcctctcc
     4321 ttctaatgac aatatagtat aaaactggtt cttggatttt ggctgacgga acaaaacata
     4381 aacgagtcct atcatcagta aaatagcaag atacgtccct aacgctacct gtccaccctc
     4441 caataagtgt cccaactgat aaatcacaaa actaactaaa taagctaaac cgcattgata
     4501 gcctatggcg atccaagtcc atttcttggc attcatttcc cgatggatag ccccaattgc
     4561 cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat gaataagctg ctgcgggagt
     4621 atatgtgtgt tgtagcactt gccagatttc tctgccattt tcggatactt ccgcatgacc
     4681 aaacaaaata ccaaatgtgc caataatatt ttctttagcg accaatcctg taatcgctgc
     4741 gactgttcct tgccagtttc cccaaccaag cggctgaaaa acaactgcca ggattcgccc
     4801 gaaaaaggct aagatacttt ggtcttcaga aacaggttga gcataaaaat tataagaaga
     4861 gctgaaccag atcacgatac tagaaacaaa gataatcgtt cctgcttttt taacaaaaga
     4921 cttgctgcgg tcataggttt gtcttaggac cgctgaccat tgaggaaaat gatatgcggg
     4981 caattccatg ataaaaggag ctgtatctcc agcaaacaaa cgtgtttttt tcaagctgat
     5041 tccagagaaa ataattgcag tcataccaag aaaataggca gaaggagcta cccaagattg
     5101 atgaggaaaa aaggcccctg ctactaaggc aataatcggc aactttgcag aacaaggcat
     5161 gaaggttgtg accatgatcg tcaaacgacg atcttgctcg ttttcaatcg tgcgactggc
     5221 catgacgcct ggcactccac atcctgtagc aatcagcata ggaatgaagg attttcctga
     5281 aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg atcctcgcca tatatccgca
     5341 gtcttccaga aagctcaagc ataaaaacaa tataatcaat tgcggtagaa atcctaacac
     5401 tgctcccacg cctgcgatga tgccgttcaa gatcagctcc tgcatccatc ccgctacttg
     5461 ccattcagct aacaatctgc caacatgttc tggtaaccat gtaccaaaca atgtgtcatt
     5521 gatccaatct gtccccattg ttcctactgt ttgaatagcc agataataga tcagccacat
     5581 cacaaatgcg aagatcggca aggctaacca acgattcgtt gcaatctgat cgattttgtc
     5641 actcatcgat aattggaaag aatcatttga cacaacacag agtgtacata gttgggtaat
     5701 aaattcataa cgttcattga ccagaatcgt ctcactatcg tcgtccaata gtttctctgt
     5761 taactggatc agttgctcga tctctttttg ctgagaaact gctatctcgt aatcctctct
     5821 taccttctga tctcgttcaa aatatttcat tgcataccag cgtttcttgc cgctttgttc
     5881 tcctagtaca tcttcaattt catgtaaagc tgcttctaaa cgtggatcat atgtacaggg
     5941 aacaggaata gaaggccgct tttttgcaga ctcctgaatc ttttcgatta gtttttcgat
     6001 tccttgcttt ttcaatacgc tgattggtaa aactggtaca ccaagaccat aggaaagctt
     6061 ttctagattg attttttttc cttctttttt gatgacatcc atcatattca gaccaatgat
     6121 cataggaatg tctaaatcca ataactgcgt agttaaataa agatttcgtt ctaaattggt
     6181 agcatcaata atgttgatga tcagttcagg ctgttcctga accaaataat ctcttgcaat
     6241 cacttcttct ggtgtataag gagacaaaga atagatgccg ggtaggtcct gaatatgaat
     6301 gttcggattt tttttcaacg atccttcttt tttttctact gttacgcccg gccaattccc
     6361 tacatattga ttcgaaccag tcaagagatt gaacaagcta gttttcccgc tgtttggatt
     6421 tccgactaac gcgatattca ttctttttcc tccctttgta tcaagattag ttcggcctct
     6481 gactttcgta acgtcagttc ataccctcgc actgtcagtt cgattggatc tcccagaggg
     6541 gctagttttc ggataactat ttttacattt cgcgtcaatc ccatatccat taaccgtcgc
     6601 ttcactgcgc cttgtccgtg gattgctacg acgatcccag tctctcctgc ttttaattga
     6661 tcaagagaag tccagctttc agaggaagca gtctttttat tgacaaagat ttgacctagt
     6721 atttcccgac ttactgccac tcgatgactt tttaccagta aaatggcatt gttgcctgat
     6781 aaattgacga cgacgatttt acttccggca acgatcccga tattttgcaa atggcgtttt
     6841 gttttttctt ctgtttcgat tcgttcgact tgataaacga aattctgctt tgcctgcgct
     6901 aaggtctgca tattgattcc tctcttgtga aataaatcat gtattaaaat cattcattat
     6961 atgttctaat tttcttacgt gttacgcaaa taaatgagaa tcattctcac atagaatact
     7021 ctcggtaatt gagaatgtca attatacttg tgatattatg agcgaatctt tagtattgat
     7081 agataaaaat gagaaaaaaa tatttcttca tagttttcca caattttttc tctattaaat
     7141 ttgctatttt cccacacgat acttgttttc agctcttttt tcaaaaacac aaaacttagt
     7201 attccacagt ttccacaaca caatatgtag tgtttgttgt ttgacttttt tactaagagt
     7261 tttatactat ctattataga taataagaat gtgttagagg aggcactcac catgaacgta
     7321 aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg tcaaacggtt tttaacagaa
     7381 aatcaaattg cttttgaaga agtcaatatc gatgaacagc ctgaagcagt agactggttg
     7441 aaagcacaag gattccaaag tgttcctgtc attacttctg atgcagaaac agttatcgga
     7501 ttccgtcctg atcagttgcg taaacttgcc agctaacatg actaaaagaa tcaaaacgtg
     7561 atacatggat ttgaagggct tgagacagca ccagtttttg tcgcaaactt ccaaatcctt
     7621 ttttaaagct taaaaacaat gcaatgattt gcaatcaagc atcatcaaat acgaaatgaa
     7681 ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt cagttatttc aagctgaata
     7741 atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa taaggataaa gaagctttag
     7801 ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac ttttctatct attatggaaa
     7861 aaatcaatta cttgatcgat caagaatata tagaagcaga attcattcaa ttgtattcac
     7921 catcatttat tgaaaagcta tatcaatttt tggacgagca acatttttct ttcaaatctt
     7981 ttatggctgc ttacaaattt tattctcaat atgcgttgaa aaccaacgac ggttccgctt
     8041 atctggaaac ttatgaagat cgagtagcct tcaatgcgct ttactttgcg aacggggatg
     8101 aagaattagc acttgctctt gcagacgaaa tgatccatca acgatatcag ccagctacac
     8161 cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt ggtgtcttgt ttcttgactc
     8221 aagtcactga cgatatgaac tctatcgggc gctcaatcaa ctctgctttg caattgtccc
     8281 gtattggtgg cggcgtaggg attactttat caaatcttcg tgaagccggc gctcctatca
     8341 aaggatatga gggtgctgca agtggtgtcg ttccagtcat gaaattattc gaagatagtt
     8401 tcagctattc taatcaatta ggtcagcgtc aaggagctgg cgtggtttat ttgaacgttt
     8461 tccatccaga cattattgct ttcctttcag caaaaaaaga aaatgcagat gaaaagattc
     8521 gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa attttatgaa ttgacacgta
     8581 aaaacgaaga tatgtattta tttagtccat atagtgtcga aaaagaatat ggtgtcccat
     8641 tctcttatgt aaatattaca gctgagtatg atcatctagt tgcaaatcca aacattcgca
     8701 aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc aaaattacaa caagaatctg
     8761 gctatcctta cgtaatcaat attgatacag cgaaccgcga gaatccaatc gatggcaaga
     8821 tcatcatgag taatttatgt tcagagattt tacaagttca aacgccttct gtgattaacg
     8881 gcaaacagga atatgaagta ttaggaacgg atatctcatg taacttaggc tcgacaaata
     8941 tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt gcgtacgatg actcgtgcat
     9001 tgacatttat tacagatgca tctgatatcg atgtcgtacc accgatccaa aacggaaata
     9061 agctgaatca tacgattgga ttaggtgcca tgggcttaca cacttatctt gcaaaagaac
     9121 aaatggaata tggctcagag gattcattag actttacaaa tatttacttc atgctgttga
     9181 attattggac acttgttgaa agcaacaata tcgctcgtga aagaaaacaa gttttccata
     9241 attttgaaaa atcagcttat gctgacggca cttatttcga aaaatacgta actggagaat
     9301 tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg catttttatc ccaactgctg
     9361 aagattggaa tgctttgaaa caagcagtaa tgaaagatgg actgtatcat caaaatcgct
     9421 tggcagtagc accaaatggc tcaatttcat atatcaacga tacgagtgca agtattcatc
     9481 ctatcactcg catgatcgaa gaacgccaag agaagaaaat cggtaaaatt tattacccag
     9541 cgccttatct atccaatgag acgattccgt attacacttc tgcttatgat atggacatgc
     9601 gtaaagtaat cgatgtttat gcaatggcac aacaacacgt cgatcaaggc atgagtttga
     9661 ctttattcat gcgttctgaa atcccagaag gcatttatga atggaagaca acaacgaagc
     9721 aaacgacacg tgatttgaat attttaagac attacgcttt ccacaaagga atcaaatcca
     9781 tttattacgt acgtaccttt actgatgatg cagaagaaat cggaagcaat caatgtgaaa
     9841 gctgcgtgat ctaatatcat agatagattc gcttattcat attttaatta aaggagcatt
     9901 ctcatgtcag aaacatatta tgaagcaata aactggaatg aaattgaaga tatcattgat
     9961 aaatccactt gggagaagct gacagagcaa ttttggctag atacacgtat cccactttca
    10021 aatgacctag atgattggcg tacattgtct caattggaaa aagatacagt aggacatgta
    10081 tttggcggtt tgacactgct agatactgtc caatctgaaa gtggcatgga tcagttgcgt
    10141 aaagatgtcc gcactcccca tgaagaagcc gttttgaaca atattcaatt catggaatcc
    10201 gttcatgcca aaagttattc ttctattttt agtacactga atacgaaaaa agaaatcgaa
    10261 gaaatttttg actggacaaa tacgaatcct tatctgcaaa aaaaagctga gcgtatcaat
    10321 gaaatctaca aacacggtac accgctagaa aagaaaattg ccagcgtctt tttagaaaca
    10381 ttcttgttct attcaggttt ttatactcct ctttattatt taggaaacaa caaattggct
    10441 aacgtagcag agatcatcaa actaatcatc cgagatgaat ctgtacatgg aacctacatc
    10501 ggttacaaat tccaattagg attcaacgag ttacctgaag cagaacaaga aacgttaaaa
    10561 gactggatgt acaatcttct ttatgagctt tacgaaaacg aagaacgtta tacagaagaa
    10621 ttatacgatc caatcggctg gacagaagaa gtcaagacat tcctacgcta taatgccaac
    10681 aaagcattga tgaacctagg gatggatcca ctctttccag atactgccaa tgatgtcaac
    10741 ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc atgacttctt ctcacaagtc
    10801 ggaaacggct atttgttagg tcatgtagaa gcgatgaaag acgacgatta tttgattgga
    10861 ttggaataag gatttatttt aattcaaaaa tttttcgaaa aataaaaaga gtttagcgta
    10921 cagatatttt aaatgtacac tgaactcttt tttatgctta ttcacttgtt tcttccagca
    10981 cttttggact gtctaacttt tccgaaacat gttccattct tctcaataaa tagattttcc
    11041 ggatatttaa tattatcgtc ttaatcactg catagacagg tatgcaaatc agcatgccta
    11101 atattcctgc gacacttcct gctccaatca ataccaagat gatcgtcaaa ggatggatat
    11161 tcattgattt cccaaataac aacggcttga tcaaatttcc atctaattgt tggatcacta
    11221 aaatcgacaa cgccatatac aaagcttgaa atggagagat aaacaaaccg acaatgattg
    11281 ctggtaccgc tccaataaaa gggccgacat acgggataat attcgtgatt ccgcaaatga
    11341 ccgcaagcaa caagctataa ggctgatcga atattctcat tgccaaaaag ctcattacac
    11401 caataatcaa agcatctaag atcgtactgc tgatataggc agataatgtt tcgtttaatt
    11461 cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg aaagaagtga gacaacgcat
    11521 caataaattt gtgcccatcc ttgaacataa atagcaaaat aaatggaacg gtaaataaca
    11581 aaataaaaaa ttgcatcata aatgaaatga tttttgagac acttgcagat aaactgacta
    11641 atatgatatt gaacaaatta ctaagagata aatttgctga tgctaattgt tcttccaaat
    11701 tgaaattttt aaactcttcc cgctgcgcca attcatttaa ccatttgctt gtttcatctg
    11761 catacattgg caagctctgg gtcaattgaa ttgtctgttc cattaactga ggcactacat
    11821 tcatgacagt taaaacaact aaaatgatta cgaccaaaaa cgtcaataaa aaaccgaaca
    11881 ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg atcgaacata taatagataa
    11941 aaccagcaac aagcaatggt aaaaaaactg ccgaaagcat tttcaatatt ggactaaaaa
    12001 tattcggcat ttgtaaaata aagtacacgc cgatgatcgt gagtactagc tccactgtcc
    12061 agaaaaataa ttttgattgt ttaaaacgtg attgcataca tctcctccaa ggtcctttga
    12121 tacttctatt ctatcattta acaaagagtt acgcgctgag attttcattt gcttaaaaaa
    12181 aacaaaaaaa ataaacatat tttaatgagt agacgagtac ttaaacaaaa acacggtttg
    12241 gagtagtgac aaaaattata ctgcagtccc tttttccaaa taaacggtgt tcaaaagctt
    12301 gcatcttcgt aataagccca gccaaacgaa aaatataagg aactattttt atttggctgg
    12361 gcttattact tgatgtagga catctatttt acaacctcag atcatttatt gcgcaatact
    12421 gactaaatgc aactttgtga tatttttgtg tacggacaac tcctcgatca catcggcata
    12481 agtgagccct tttggaagat caatcgtata gatatttttg tacactcggt agtcatcaac
    12541 gaattttacg tcaaaattca catcttcgat tacgatattt ttctcttcaa aataatcggt
    12601 aagaaaatct ttggtttctt ttctatggat aaattggatt tctaactttt tagtcgtagg
    12661 tacatggatc acgcgtttca ctactgtgag tgcaatagca atcccgataa aactgatgat
    12721 cgcaatggaa tagtaaccca tgccgatggc gatccctaat ccagcaattg cccataaaga
    12781 agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg atgatcgttc ccgcgcctaa
    12841 gaaaccgaca ccactcacca cttgagcaat cagccttgct tcatctgaac gaatcgttcc
    12901 agcaagctgt ggatactcta aagcatctct taatgcacca ctagcgatct caacttggat
    12961 caatgcaatg atacatgctc ccatacaaac taaaatatgc gtacgcatac ctgctggacg
    13021 atttttatat tgtcgttcaa aaccgattgc tccgccaaat agcatagcga tccctaaacg
    13081 taaaataatc tctggaattg tcaataccat atcattcatc taaacaccct ccagtccaat
    13141 atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt ttacctctta cgaaaaaagg
    13201 actgtgacaa aatttttgtc atagtccttt ttatgaataa acggtgttca atcagctgac
    13261 cctcggtatt actcaacaaa cggcaaaata tggggagcta tttttccttt gtcttctcta
    13321 atactcaggt caaaacgctg atttcacaac ctctttagcc tactattgaa aattacaact
    13381 tacttgctgc ttcttcatcg atgatcacaa caacattgtc gtgattttgc aatacacttg
    13441 ctggcaaatg attcgttaca ggtccttcaa tcatcccttt gattgcatca gctttgcttt
    13501 cgccaaatgc catcaagacg atttctttac ttttcataat tgaaccgatt cccattgaaa
    13561 cagcttttgt tggtacatct tctgctttat cgaagaatcg tttgtttgca ttgattgttg
    13621 attctgttaa gtcaactact gaagtcgttc cgtcaaatga agcaccaggt tcattgaaac
    13681 cgatatgtcc attttgcccg attcctaaga tttggatatc gattggatgt gcatcgatga
    13741 tttgatcata ctttttgcat gctgcttcca gatcttctgc ttttccatct ggtacaaacg
    13801 tttctttgaa tggtttttta tcaaataatt ggacatccat gaaatgacgg tagctttgtt
    13861 catctgaacc gcctagtcca acgtactcat ctaagtttac agaagtcatt tcactgaaat
    13921 ccaaatcgct tgaagtcatt tctttatata aagtaactgg tgtgcttcct gttgccaatc
    13981 ctaatacttt tgcacctttg ttcatacctt ctttgatgat ttcaaatgct ttttttccgc
    14041 cttcttcagc gttttttaca cgaatgattt ccatagtgat tgctccttta tattttggta
    14101 tagcccaatt atagcattct ttttttcttt ttacaataat tagtatagac caaattgcat
    14161 ttttttcatt ttcttgacaa taaataggaa tccgtgttag gtttcataag atggttcaat
    14221 caaacaatcc atgttgcatg aaaaccaagc aagtgattca ctgtggacgc tttatgtctg
    14281 cagttttttt atgaaaaagg agttattatg agtatactga cattaaacaa cattacccag
    14341 caatttggcg acaaaatcct ctatgaaggt gtcgatctgc aattaaatgc tggcgaacat
    14401 cttgggctga ttggacaaaa cggagccggt aaaagtacat tgattaaaat catcactggt
    14461 gagatcttac cagatgacgg acagatccat tggcaaaaaa atattcacaa aggttacctc
    14521 gatcaatatg tggaagtcga tgaaacatta acaatcgaag aatttctaaa aactgcgtac
    14581 gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt accaaacata tagcgagacc
    14641 ttcgatgatt ccttgctgga aaaagctggg aaactccaaa cggaattaga ccaaggagtg
    14701 ttttatcaaa tcgacacctt agttgctgag atgagcagcg ggttggggat cgatgtctta
    14761 ggtttagatt cactgttgaa aaatctaagt ggtggacaac gttcaaaggt gattttagca
    14821 aaactacttt tagaaaaccc agatgttctg atcttagatg aaccaaccaa tcacttagat
    14881 gatcagcata tccaatggct gacgcagttt cttcaggatt ttgacggtgc gtatatcgtg
    14941 atttcccatg accaagaatt tcttgatcag atcaccaccc atatcgcaga catcgagttt
    15001 ggaaagatta ctaagtatac tggccatcta aaacaagcat tgaaacaaaa ggaacaaaat
    15061 cgcgaaagct atttgcgcca atatcatgct caacaaaaac aaatcgaaaa gaccgaagct
    15121 tatattcgaa aatacaaagc cggttcccga tcaacgatgg caaaaagccg acaaaaacaa
    15181 ttagataaaa tcgaacggct gacgcctcca gcttcagcaa gtaaagctgt ctttgatttt
    15241 ccattctcgc ctattgtcac gacattagca gtggaaacaa ctgatttagt catcggatat
    15301 gatcgaccgc tacttgcacc catcaatttg accatccgct ttggtgaaaa tattgccatt
    15361 cgaggcttca acgggattgg aaaatctact ttattaaaaa cactgatcgg tgagattgaa
    15421 aaaataagtg gcgatttcca cttcccagat aacacaaaaa tcaattactt ttcacaagat
    15481 ctgtactggg aaaatccatt agaaacacca ctacaatatt taagcagtca atttccaaaa
    15541 gcgactatca aagaattacg tcgccaacta gctaaagcag ggttagttaa tcaattagca
    15601 agtgaacctc tcactacatt aagtggtggg gaacaaacta aggtgaaact tgcacagttg
    15661 acaatgaacc aaggtaattt actgattctc gatgaaccga cgaaccatat cgatcaagaa
    15721 acgaaagaaa gcttacaaga cgggatacag aaatttccag gaacagtaat catcgtctct
    15781 catgaacaag aattttatca agatcttgtg caacgagtca ttgagattga accaaaataa
    15841 acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa agttttgtca caactccttc
    15901 ttctgaataa atggtggcca attagctgac cttcggtatt agctcaaaaa acggcaaaat
    15961 atgaggagct atttttattt ggctattctt attacttgat gcagaacagc ttttcacaac
    16021 ctcttttcgt ctatttttta ttttcgagat ttctctttcc tttaaaaaca actgtttgaa
    16081 agaagaacgc acatccttga aaaacaagcc cgctaaccgt tacacctaac cagccatact
    16141 gctgccacat tgtcgtccct agcaatgaac cagccgcacc tccaataaag tacataaaca
    16201 taaatacagt attatttcgg ttgcttgctg cttcacctag cccttgtact cgtgtttgat
    16261 tggcgacttg accaaattga ttcccgacgt ctaataaaat aatagctaaa agcaaaatgg
    16321 ccaaatggct tccgccaacc aataaagcaa taaagcttat tgtttgcata acaagtccca
    16381 tatacacgat ttttctttca gaataagaat ctgccagtcg tcccacgata ggtgcagcaa
    16441 ctgctccaga caaaccaaaa atcgctaata tccctgcttc gaatgttccc caattgtagg
    16501 cgggactgct gatataaaaa atcaacgtag accaaaagat cgaaaatgtt ccaaacatga
    16561 aaaatccact gactgccgct tctcttaata gacgttgaga agcaactaaa tgcggcaaac
    16621 ttttaagtga atcaaggtat gaaacagctg tactactttg ctggacagaa cgcggcattt
    16681 tccagtaaag cagaagtgta agaattccga ctgcacataa agcaaataaa tagatcatcc
    16741 gccaagaaaa aagactggca acaataccag ataccgtccg agaaagcaaa atccccacga
    16801 gtaaaccgct aagcaatgta cccatgacct tccctcttgc ttttggtccc gctagtaccg
    16861 caccgtaagg aatgatgatt tgcggcacga tcgaaagtaa tcctattaaa aaagaagcgc
    16921 atgcaaataa cgtaaacgaa ggcgcaaaaa aagcggccaa cagagagacg gcagataacg
    16981 ctgccattcg aatgatcaat tttggacgat ccaccacgtc ccctaaagga actaaaaaca
    17041 gtaaccctaa cgcatagcct aattgggtca acatcgtcaa tatcccgact gcacttgtgc
    17101 taacagataa actagctgct acttttgttc caatcggctg gatgtaatac atattggcaa
    17161 caacaacgcc acaagtgatt gataatagta aaataagcga agttgttaaa ggtttttttt
    17221 cattactctt cattgatttc ccccatttcc tatcttagtt tacaacttat ggaaaagatt
    17281 ggaaatagaa aaatacaact attcgccaca cttattattt gtgcttttgt tttccttgtt
    17341 ttttcttgcg atctttataa atgaagacaa ctaaccctac tagcacagta acacttgcaa
    17401 ccaacgtgac tgcagttttg ttatatccgt gaaacagagc ttctccacca cacgcataca
    17461 acacagacga agggatgact ccaatcacga tcgacaatat gatttgctgt agaggcaatt
    17521 tcatcgcatc tgcagcaaaa ttgaccatag aagatggaat aaccggtatc atatagccta
    17581 acattacacc tatttttgga tgtttcattt gtcggattgc ttgcacccaa tgattcgttt
    17641 cggttttctt atctaaaaat tttaagcgat gcataaggaa aattgcagtg atgttaccaa
    17701 gagcattacc caaaatgttg atgatcgcac ctgtcagtgg tccaaagctc aaaccagcaa
    17761 tgactccaac tactgaagta ggaatacctg gaacagcaca catcaccgct gtcaatacga
    17821 tcaagagtat cgatgcaaaa gtcccatgcg aacgaatatg tgcgagtagc tctgccttac
    17881 tcgctctcgg atttaaaaat aattggatgt ctgtctgata ttctaaatac aaccgatagc
    17941 ccactaccag tatcagaatg attcccaacg agatcaatat caatcggatt attttctttt
    18001 tcatatttcc tcctgaaaaa aatctatatt cattaaataa tagtttaaca taaaaacaaa
    18061 aaagcaccga aatccaacat aggatctcag tgcttttaaa tagtaaaaat tatttaacag
    18121 ttacagatgc gccaacttct tcaagtttag ctttcaattc ttcagcttct tctttagata
    18181 cgccttcttt aactggtgca ggagcgccat caactacagc tttagcttct ttcaagccta
    18241 aaccagttgc ttcacgaact gctttgataa ctttaacttt ttggtctcca gctgcagtta
    18301 attctacagt gaattctgtt tgttcttcag cagcagcagc gccaccagca gcagctacag
    18361 ctacaggagc agcagcagat acgccaaatt cttcttcaat agctttaact aggtcgttaa
    18421 gttctaggat agttgcttct tttaactcag caacgatgtt ttcaatgttc aatgccatta
    18481 taatttcctc cgtttaattg ttctttttta tttaatgctt tatgcggcaa ttaagctgca
    18541 tctccatctt ctttgtctgc cactgctttg acagcgtaag ccacgttgcg gactggcgct
    18601 tgtagtacag atagcaacat agatagaagt ccttcgcgat ttggcaattt tgccaatgct
    18661 gtgatttctt ccaatgaaga gactttacct tcgatgatac cgcctttgat ttctaatgct
    18721 tttgcttctt tagcaaattc gtccatgatt ttcgctggtg cgactacatc ttcgttactg
    18781 aatgcaacag ctgtaggtcc agtgaatact tcgtccatgc cttcaaggcc agctttttta
    18841 gctgcacgtg aaagaataga atttttgata actttcattt ctacgttagc atcacgaagt
    18901 tgtttacgta aacgtgttac ttcatcaact gttaatccgc ggtagtcaac gactactact
    18961 gatgcagctg ctgtaaattt ttcgcttact tcatcaacga ttgctgcttt ttttgcgata
    19021 attgcttcac tcatttttga gtttcacctc ctgaattttg atggaagagt tttttgcact
    19081 gaaaaggcaa gtcactttta tagttacttt gctcaaccag tattattcat cacctattca
    19141 cttcgttcat agtgattcta ctcgctttgc tcgaaaggaa actcctttcg ctttgctcaa
    19201 ccagtattgt tcatcatctc ttcacttcgt tcatagtgat tcacaataaa aaactctatg
    19261 ccaccgcaga catagaggga ctattgaata tattcaataa acgtcctcgg taggaaatta
    19321 aggcttgatg ccacctactg tcttcggtac aagttaatta ttaacctcaa ctagcttagc
    19381 atgctaaact agttgagtca agaattattt ttcagttgga tcaatcaaca ttctaatatt
    19441 taattagaaa gaagcttggt caacatggat accaggtcca aatgttgttg tgatcgttac
    19501 atttttcatg tattgacctt tagctgctga tggtttagct tttagcaata catcatggat
    19561 tgctgcaaag ttttcaacta atttttcatc gtcaaatgaa acttttccga ttggtacatg
    19621 gatatttcca gctttgtcca cacggtaagt tactttacca gctttcactt cgtttactgc
    19681 ttttgttacg tccattgtaa ctgtaccagt tttagggttt ggcattaagc cttttggtcc
    19741 taatactcgt cctagacgac ctacagtagc catcatatca ggtgttgcaa caactacatc
    19801 aaagtcaaac catccgtttt ggattttttg aaccatgtcg tcgtcaccta cgaaatctgc
    19861 accagctgct tcagcttctt ttgctttgtc tcctttagca aaaactaaaa cagtttgtgt
    19921 tttaccagtt ccgtttggta atacaacagc accacggatt tgttggtctg cttttttagg
    19981 gtctacgttc aaacggtatg caacttcaac tgttgcatca aattttgcaa tgtttgtttc
    20041 ttttgctaaa gctactgctt cggcaacagg ataaacttta cttgaatcaa ctttttttaa
    20101 tgcatcttgc atttttttgc tctttttagc cattttggtt cctccttgat tgtggttata
    20161 acggttgtac ctcccacgtg ttctatatct ttcaatatag agccaactga gaagctgctg
    20221 tcttagggtg tatagattat tctacagtga tccccatgct tcgtgcagtt ccttcaacca
    20281 tgcgcattgc tgcttcaacg ttagctgcgt ttaggtcttc catttttaat tcagcgattt
    20341 ctcttacttg atcgctagaa actttcgcaa ctttattttt gtttggttcg cctgaacctt
    20401 tttcgatttt agctgctttt ttcaatagaa cagcagctgg cggtgttttt gtaacgaatg
    20461 tgaatgaacg gtcttcgtat acagaaataa caaccggaat gatcaaacct gcttgatcag
    20521 ctgtacgagc gttgaattct tttgtgaatc ccatgatatt gatacccgct tgacctagtg
    20581 caggacctac tggtggagct ggagttgctt tacctgcagg aatttgcaat ttaacgattt
    20641 tttctacttt ctttgccacg agacatacct ccttaagtcc gtgatgtggt taattggaga
    20701 tgcatatttc tcctcccacg ttttatctga tctgaaaaaa tccagacaaa taatgtgcaa
    20761 aacaatgcac actggtacag tatagcaaat ttatttgttt tttcaagatt tttttctgct
    20821 atttttacat tttgcttgaa taaacgctaa atttattcta aaaaaacaac aatttttcgt
    20881 tctcatttct tcttttatgt tacacttgcg ctagcatggt tcaacagatg aaagcaaaca
    20941 ataacacggt tttttagacc cttgtacaaa gattcctaca taacaaaatg aggtggagaa
    21001 aatgaaagat ttaaggtata gaagtgtctt tgatattatt ggacctgtga tgattggtcc
    21061 aagtagctct cacactgccg gagctgctag aatcgggaaa atcatacgta gtatatttgg
    21121 cgaacagcct gattctgtag atatttatct atatgaatca tttgcaaaaa cctatcgggg
    21181 gcacggaaca gatattgcat tagttggcgg cctgttaggg atggaacccg atgatgaacg
    21241 tttagctgat tctttgaaat tggcatatga agccggtatg gaagtcttgt ttgttcctaa
    21301 gagtgaaaaa gccgaacatc caaactctgt aaagatgctt ctaaaaaaag gaacacgaaa
    21361 gctttcagtc actggcattt cgatcggcgg tggcaatatc cagatttctg aattgaatgg
    21421 attcaaaatt tctttaagca tgggtactcc taccttgatc gttgtccacc aagatgttcc
    21481 aggtatgatt gctaaagtga ctagtatact ttctgagacc aatacgaata tcggcactat
    21541 gacagtgaca cgggaatcaa aaggtgaaaa tgccattatg ataattgaag tagatgatcc
    21601 tcaagtagaa gaaacggtaa aaagactcaa acagctgcca aatatcgaca gcgtaaatta
    21661 tttcgattaa ggagctaaaa aaatgttttt atcgattgaa gaattagtag atcaagcaaa
    21721 agcttatcca agtgtagcag aattgatgat tgcagttgaa atggaaatga gtggccgttc
    21781 ccgtagtcac atcatcgaga tcatggaaaa aaacctctca gtaatggaac aatcaatcaa
    21841 cgagggaaaa gaaggtgtta cctccgtcac aggaattacc ggtggtgatg caaaaaaatt
    21901 agatgattat ttaaatgagg cagacttttt aagtggagaa acaatcttaa aagctgtacg
    21961 caatgctgta gctgttaatg aagtcaatgc aaaaatgggg ttgatttgtg ctacaccgac
    22021 tgccggaagc gcgggtgttg ttcctggtgt gctgatggct gtccgagatc ggcttgattt
    22081 gacacatgaa cagcaattgg aattcttatt taccgctggc gctttcgggt tagtcatcgc
    22141 aaacaacgct tctatcagtg gtgcagaagg gggatgtcaa gcagaagttg gttctgcaag
    22201 tgcaatggca gcagcagctt tggtttgtgc aaaaaatggg actcctcatc aagcagcaca
    22261 agcagtagcc attacattga aaaatatgat gggactgatt tgcgatcccg ttgcaggctt
    22321 agttgaagtc ccgtgtgtca aacgtaatgc tttaggctct tctcaagctt ttatctctgc
    22381 tgatatggca ttagcgggta tcgaaagtgt cattccgccg gatgaagtcg ttgctgcaat
    22441 gtaccaagtc ggacgtcaaa tgccgtcgat tttcaaagaa acagcagaag gtggcttagc
    22501 tgttacgcca actgctaaac gacttgcgga agagatttta aataagaggg cgtgataaag
    22561 ctccttaatt tcgagtatta agagattagt tatcttcata acaaaaaacg aagagaggac
    22621 taattgccct tctcttcgtt ttttattaat ccatcaattt cgtatttttt acttcatcga
    22681 tcgtttttgt tcctgcaagt tgcatcgtga tcgacaattc tttgttcagg tgttcaaaaa
    22741 cagatgttac tccttctgcc ccacctagat tcaacccata aatcactggt cggccaatcg
    22801 caactaagtc agcaccactt gccagagctt tgaagacatg ctctcctcga cggacaccag
    22861 agtcgaaaat cactggtaca cgtttgttga ctacttcggc aatttttggc aatacttcaa
    22921 aggacgcagg acctccatct agttgacgtc caccatgatt ggatacccag ataccatctg
    22981 cccctgcaga gatagcgatt actgcatctt ctggcgattg tattcctttt acgataactg
    23041 gaagatgggt gatttctttg attgttttga tgtcatctgg tgtcaagcct tgttttgcag
    23101 ctgcataaat ttctgcgatt ccttttcctt cgccattacc acttgcactt tgttcgctat
    23161 acgcagctaa gttaggcatt ggtaatggaa attggaattg attgatgacg tcttcttcac
    23221 ggtaaccacc gagcgttgaa tctgccgtta aaatgatagc tttagcccct gcttctactg
    23281 ctttgttcaa aatgaactca ttgaatctgt cgtctttact catatataat tggaaaaact
    23341 gcggtgcgcc tggcgctgca tcggctgcgt ctttgattgt cgtatttgca taagtgctga
    23401 tggaaaagat tgatccagca gcagctactc ctttagctgt atcagcttcc cctttttcat
    23461 gtgccaatcc ttgggccgct gatggcgctt ggataatcgg tgtatctaat tcaattccga
    23521 aaacagatgt atgcaaatcg gctgagtcga ttccgcgtaa tatccgtggc atgattttct
    23581 ttgtattaaa agaagtcgta ttttctttca tcgtccactc atcttcagaa ccaccgcgaa
    23641 tatagccaaa agctcctgct tccatgcgct cttttacacg accttctagt taggcaatgt
    23701 ttacaatatc gattggatgt tcttcattac ttgtatgata agtcatagta gatccttctt
    23761 tcatcttctt gattacatat aaatcatact aaacttacaa aaagtaagca aacatttact
    23821 ctcagatact tttatcgcta ttctttccaa ataaatcgac ttacaagctg atctacttct
    23881 tggttttcat gaagcatact atgagaggca ttttcacctg tcactacttt ttcctctatt
    23941 tcatttccgc gttgcttcaa caacgcatag accgctaatg cactgtttaa cggtactgtt
    24001 ccatcactca agtctgtttc atctagttga ccagcaatca agaaaaatcg agtggttata
    24061 ggaatattac cgatcatttg ctggtaatct tgatagcggt tagaaacgac agcaggcccc
    24121 tttgctaact catctgtcag agactgcgca ctatcatcga caaaatcatt aaaaggcgcg
    24181 ccaattgctg ttaatgtctg aacttgtggc aatgacgtat cctgtccata cgttcctaaa
    24241 tagcgtagtg cactaactcc tcccatggaa tgccccacta tgttgacttt atctatgccg
    24301 tagttttctt tcagatacaa aagacaagtt ttgatccatt ctgcttgatt ccactcgtta
    24361 tttttattat cttcaaacaa tacttggacc atcggattgt ctgcttttcc agacaaatct
    24421 cctgttgctt gtatttgtcc atctgttcct actgttaaaa ccaattcttt tactgcttgc
    24481 tgattttttt ccaaacgccg caacatccct ttaaaggaat tttctgtccc gctgtagccg
    24541 tggaaaaaaa gtgttggaat agtggttttt tctatcactt tactggattc tgccgttttt
    24601 tctttatcag aaacttcagt agtatttatc ttcgtcgtct ggcaaccact taacgcaaac
    24661 aaactgataa ggactaaagc tgctgcagtt gcacgtttgc ataaattttt gattggttct
    24721 gtcatacacc tcttcctttc catatagaaa aaagaaagac ttgaaaggcc aatcatatga
    24781 tcgacatttc aagtctcttt attaatcgat tttatctact tgatcgaaat caagttctgt
    24841 actggtttca cgtccaaaca tatcgatgtt gactttcaat ttttcttttt ctccgtcaac
    24901 ttctgttact tgtccttcta atccagcaaa tgcgccttcg atgattttca cgacttcgcc
    24961 aacttctact tctagatcat tttgtcgcag gctcataccg atagaacgca agatatgatt
    25021 gacttcttct ggaagtaatg gtgctggttt gcttccggct ccgtgtgaac caacgaatcc
    25081 tgtcacacca ggtgtgttgc ggacaacata ccatgaagcg tctgtcatga tcatttcaac
    25141 aagtacgtat cctgggaatg ttttatgaac gatttctttt tctttcccat ttttgacttc
    25201 agtttctttt tcttcaggaa caacgacacg gaaaataaag tcggccattt tcatactttg
    25261 cgcacgagat tcgatatttg cttttacttt gttttcatat cctgaatatg tatgcaagac
    25321 ataccagttt ttttcaaaag attccattga aggctcctca tttcattgta aaataaaaaa
    25381 acctcagtac acccgaagtt tttctttcgc ttcattatag cactaaaagt acgttgtggc
    25441 tagcgagtta ttttaaaatc cagccaaata agctttggat ggctgtatcc attacgtaaa
    25501 acatcaatcc gaaaataata gaagtttcga tgactactaa cgtatcttta cggagttgtt
    25561 ttctgcttgg ccaagttact tgtttgattt catcttttac gctttttaga aatttcatga
    25621 gacaacctcc cttgctactg tttatttcgt ttctttatgt aatgtatatt gattacaata
    25681 tttacaaaac ttattgattt ccaaacgttc gccgcttttt ccttcactga ctgattttgt
    25741 gtagttgcga gacccacaaa cggaacaagc gagtgctgct tttttctttg ccatttactt
    25801 ctgccccctt gctgagaatc agacttatac ttcataacag tatcacgtaa ctttttactt
    25861 gtcaatttgt gaaataaact atctattgtt ttggataaaa ttatgatatg attaatttga
    25921 aattaagcta aaatgagaca attggaggta tagcgatgct attaaaaaca cttgtatata
    25981 agaaaaaaga tctgacaacc gttacagaga ctagtacatt agaagaagca ttaaaaattt
    26041 tagaaacttc cggataccgt tgtgtgccga tcttagattc ttcaggtaat attttccgtg
    26101 gaaacattta caaaatgcat atctatcgtc ataaagcaaa tggcggtgat atgacgcttc
    26161 ctgtcactca tcttttgaaa aacgctacaa agttcattta cctggatact tctttcttca
    26221 aagtattctt tacgatcaaa gagcttcctt atattgctgt ccttgacagt gaaaatcatt
    26281 tttatggtat tttgacacat agtacgttgc ttaatatgct ctctcaatct tggagcgtag
    26341 aacaaggaag ctatgtactg acaattgctt ccacaggaaa gcaaggagat ttggctgcta
    26401 ttgcaaaaat catttcaaaa cacagcagca ttgccagctg tattactctt gatatcgaaa
    26461 aagacgaata cattcgacgt acattgatca cgctaccggc taacactgaa gcgaaagtat
    26521 gtgaaacgat cgtcaaccac ttagagaaaa agaattataa agtggtagaa attgaaaatt
    26581 tgcaaaacca agaagcataa tcaaaaagcg aaaaaagaaa acagctaatc tgctttcttt
    26641 tttcgctttt tataattaga cacgcttttc aaaatcaagc gcgcgatcta aataatacat
    26701 caatggtgct gaaacagcca tgacgataaa ctcacttaat gcaagcgtgc cataggttgt
    26761 ccaaaatgct tggccacctg ttggaactaa catataagca atcagtccca tgctgatggt
    26821 aaagaagatc gtgttcaata ctaaacgcat ctttacattt ttcactttgt tttgcaataa
    26881 agctgtcagg cctaaagaaa gcaatgtttg gccgccacca aaaaccacat ccatgatacc
    26941 aaaaccaaag aaggcattat agacgatgac cccgcctaaa actccccaaa gcaatttacg
    27001 gttaaagaca actaaatgat tcaaactttc tgaaatacga aactggatcg gccctgagga
    27061 tacaggcgca actaaaatag ttaacgctag ataaagagcc atgatgatgc catttacaac
    27121 gattacggat aaccgttgtc tgcttttcgt tttttgcatt ttctctcctc ttttctccta
    27181 gtttttttaa cgcgggatgg ttgatgaacc gcggttatgt gaaacaaaca ataattataa
    27241 cagacttttc atttatttca atccgtctta tctgctttta aaaaagcaaa agcttaaaca
    27301 atgtaaacgt ttgctaaaaa tcgtgggata tggtataaag taagtgtaag gggtcataag
    27361 aattcccagt aaagcagacg aagtaggatc tgcaagaaca gaattgttgt gaaacttaaa
    27421 taaataaaaa aggatgtggg aatttgagca acctcttact agagaacggt ctagcaagca
    27481 gctgaagatc taagtggaca tgccgaacct tggaacttct ccttacgtaa gtaatggatc
    27541 atgcgcttgc taggcttttt tgtgtttttc ggtcttcgga cctttttcat tttcctagac
    27601 ttctgctata ataaccaaga attaattttg taacagattt ttttgaaaaa acaagtttct
    27661 ttcattcaaa aaatagaaaa agcttgctaa ttattggtta accagcaaac aggagtatta
    27721 aaatatcttg ttttattgaa tagcaggagg aaatcatgaa attccattat catatattga
    27781 tcaatccatc ggcaggcagc ggaaatggct ataaagtagc agaaagaatt ttaccggtat
    27841 taaaaaataa gcatattgac tatacacttc attatagtga atacaaagga cacgatgctg
    27901 agattgctga aactttggca aaagaaacat tgattccttg gatagaagaa gaacagaaaa
    27961 caatcgatac tttccccctt ttgatcatcg taggaggtga cggcacctta catcaggtac
    28021 ttgatacttt ctatcagatg gaagtagaat tcccagttgc ttatattcca gcaggttctg
    28081 gagatgattt tgctcgaggg gcaggccttc caaaaaatcc caaaaaagga cttcagctga
    28141 ttttagcagc tcaatcacca gaaaaagtcc acataatggc ttatgaagaa aaaataagtg
    28201 aaaaaaaagg aatagctgtt aataatttcg gtataggatt agatgcagct atcgtccatg
    28261 cgacaaatca ttcttccaca aaaaagcgat tgaacaaata caacttagga tcattttcgt
    28321 atttattctc tattctccgt gctttattca ctcaaaaagg atttccaatt ctggtagatg
    28381 caggaggaaa acaattaagt ttttcaaacg catttttatg tacagcaacg aagcatcctt
    28441 tttttggcgg aggaatcgcg attgttccaa ctgctgatgc gtctaaacca gtcattgatt
    28501 ttgtcgtggt cgaacgaatc aatttcttta aaatcttgtg gctcattctt ttacttatac
    28561 agaaaaaaca attgaaatcg aaatatttcc atcactacac aacaaacaga ttacgtatcg
    28621 tttcaacaac accgcaacat ggtcaagaag atggagaaga aatggaaaaa cgaccttttg
    28681 atatcacaat aaccacaact aaacagttgt tttggtgtta ataaaagagg ttttgtcaga
    28741 agctacatct ggaacaccgt ttattcggaa aaaaggactg tgacacgact tttgtcacag
    28801 tccttttttt actatgcagt taattctttg cccaattggg ttgccatcga tagtttcttt
    28861 ctttacccaa aataaattgg atcattgaaa acaccgcagc aaaagcaact aagacacaac
    28921 cattgaacca aaaagattct gcaaatgttt gattgtaact gagtgtttca ctgatcaagt
    28981 tattattttg aacaaaccaa tatgttggtg tgaatgccgc tattttgcta accatttctg
    29041 gcaagtattc tgctggtaca aacaccccac cgataaagca actgcctgtg ataaaaatat
    29101 ctttgatccc accgctggtt tctgagtttt ttaccaatga agtgatacaa gccgaaaagc
    29161 taatgatcgg caataaaaac aagaaagtat ttaaaatatg ataccatacc gttatatccc
    29221 acgaggtgtg gatcactaac agaaccatta gaagtaagaa gccactgaac aacagcagcg
    29281 agtaactaat caaactaaag gttactttgc gattcaagtt tcgtttggta atgggcgcac
    29341 tctggtttcg tttactgatc tctttgcgat taaatgctag attgatgacg ctgaaaccgc
    29401 taaaaatgga tagaaacaac ccataagaca agaaattaaa ggttgtgccc gtcaacagct
    29461 ttttcatccg ctgactatat cccgcactta gctcaacttc tccagtaacc gagagtgcca
    29521 tttgtgtctg ttccatagct gcatcgattg ttttatcttc ttcttgaaac agaatcagtg
    29581 tttgcaaaaa ggtgttcact tgctgtgtaa caactgtttt agaaaaggca tctgggcgac
    29641 tctgtatctt gatcgtcggc atctttccta cttcgacttg ttcggtaaag tctgcaggca
    29701 gatgtaagat aatgtttaca cgattaaaat aaagagcatc atcgatttct ttttgtgagg
    29761 ttccatccaa tgtaacgact gtttccttgt cattcaaata tttacgaagg ccatcgacta
    29821 caggcgactc ttcttcacta atcaaggcga ttttcgcatc ttgcagagag gtattttcaa
    29881 catcaatatt ttgcgcaaaa aagaatgtgg caacaacagt gataattaca cctatcagta
    29941 aattcgattt atttgcttta agcaatgtcc aaatcgtttt ataaagcgtc ataacgcacc
    30001 cctctttcca tccaagcatt tcctaacgca aaaaggaaaa taaatccgat caaccataga
    30061 agattggtat aaaaactagc caaatcagtg tagaaataaa gttt
//
LOCUS       DAZKBH010000021        29983 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000021 DAZKBH010000000
VERSION     DAZKBH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.021"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..477
                     /locus_tag="ACG8OO_000950"
     CDS             139..477
                     /locus_tag="ACG8OO_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044873.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="ACG8OO_000951"
     CDS             complement(682..1257)
                     /locus_tag="ACG8OO_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFP9044874.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="ACG8OO_000952"
     CDS             complement(1989..2570)
                     /locus_tag="ACG8OO_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9044875.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="ACG8OO_000953"
     CDS             complement(2753..3379)
                     /locus_tag="ACG8OO_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9044876.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="ACG8OO_000954"
     CDS             complement(3401..4732)
                     /locus_tag="ACG8OO_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFP9044877.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="ACG8OO_000955"
     CDS             complement(4887..5288)
                     /locus_tag="ACG8OO_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9044878.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="ACG8OO_000956"
     CDS             complement(5374..5580)
                     /locus_tag="ACG8OO_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044879.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="ACG8OO_000957"
     CDS             complement(5675..7177)
                     /locus_tag="ACG8OO_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFP9044880.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACG8OO_000958"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACG8OO_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9044881.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="ACG8OO_000959"
     CDS             complement(7748..8365)
                     /locus_tag="ACG8OO_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9044882.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="ACG8OO_000960"
     CDS             8586..9959
                     /locus_tag="ACG8OO_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9044883.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="ACG8OO_000961"
     CDS             10034..11146
                     /locus_tag="ACG8OO_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9044884.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="ACG8OO_000962"
     CDS             complement(11218..12855)
                     /locus_tag="ACG8OO_000962"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9044885.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..13631)
                     /locus_tag="ACG8OO_000963"
     CDS             complement(13023..13631)
                     /locus_tag="ACG8OO_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9044886.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14015..15166)
                     /gene="queG"
                     /locus_tag="ACG8OO_000964"
     CDS             complement(14015..15166)
                     /gene="queG"
                     /locus_tag="ACG8OO_000964"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9044887.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15185..15937)
                     /locus_tag="ACG8OO_000965"
     CDS             complement(15185..15937)
                     /locus_tag="ACG8OO_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044888.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16078..17028)
                     /locus_tag="ACG8OO_000966"
     CDS             complement(16078..17028)
                     /locus_tag="ACG8OO_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP9044889.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17029..17868)
                     /locus_tag="ACG8OO_000967"
     CDS             complement(17029..17868)
                     /locus_tag="ACG8OO_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9044890.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17865..18611)
                     /locus_tag="ACG8OO_000968"
     CDS             complement(17865..18611)
                     /locus_tag="ACG8OO_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9044891.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18849..20219
                     /locus_tag="ACG8OO_000969"
     CDS             18849..20219
                     /locus_tag="ACG8OO_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9044892.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20274..21170)
                     /locus_tag="ACG8OO_000970"
     CDS             complement(20274..21170)
                     /locus_tag="ACG8OO_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9044893.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21284..21454)
                     /locus_tag="ACG8OO_000971"
     CDS             complement(21284..21454)
                     /locus_tag="ACG8OO_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044894.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(21451..22128)
                     /locus_tag="ACG8OO_000972"
     CDS             complement(21451..22128)
                     /locus_tag="ACG8OO_000972"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9044895.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22219..23124)
                     /locus_tag="ACG8OO_000973"
     CDS             complement(22219..23124)
                     /locus_tag="ACG8OO_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFP9044896.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23299..24753
                     /locus_tag="ACG8OO_000974"
     CDS             23299..24753
                     /locus_tag="ACG8OO_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFP9044897.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            24769..25800
                     /locus_tag="ACG8OO_000975"
     CDS             24769..25800
                     /locus_tag="ACG8OO_000975"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9044898.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25807..26994
                     /locus_tag="ACG8OO_000976"
     CDS             25807..26994
                     /locus_tag="ACG8OO_000976"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9044899.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27150..28532
                     /locus_tag="ACG8OO_000977"
     CDS             27150..28532
                     /locus_tag="ACG8OO_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9044900.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28504..28890
                     /locus_tag="ACG8OO_000978"
     CDS             28504..28890
                     /locus_tag="ACG8OO_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044901.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29090..29758)
                     /locus_tag="ACG8OO_000979"
     CDS             complement(29090..29758)
                     /locus_tag="ACG8OO_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9044902.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8404 a         6141 c         5483 g         9955 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    13621 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    13681 ttatcattta gaaatatttc ataggtaaaa gaaacatgac atttaatgac aaaataacga
    13741 attatttaat atttgtaaca tttaaacaaa tatttttttg atacaaaacc ataaaagcct
    13801 tgttgtataa gcaaagtaat cgtttttcta tgttttttta tttttgaatg aaaaacgcag
    13861 attctgtaac attcactgca ataatccata aaaaaaagca aaaaaatttt tgatttttat
    13921 caaattctct taaaaaaatg taaaaaatac aaaaaaacca gtaattgtta caacaattac
    13981 tggacaaaaa ctttgaataa tgttaactat ttttttattt taactttctc cgcaataatt
    14041 tctgggcatg ttccaattcg tttataacga cttcttctgt ttctttagct aatatttcat
    14101 ctaatacatc aagccatttt tccggttctt ttgttcctag acgtccaata gcccaagctg
    14161 ctgttcctct gatgacaggg cggacatctg ttaaacattc tgaaatctca ggcaatgccg
    14221 ttcgatcacc tagattagct aaagcaatca atgcgtttct ctgcaaaggt ttcttcccac
    14281 gccatgaacc agctaaatgc ccaaattgct gtttgaagtc tttattagac atcgaaagca
    14341 ttggctttaa ttttggatat acttcgtcta cttttggttc catctcttca tgaaaatgaa
    14401 aatcttttcc ttgattgtaa gggcagacta actggcagat atcacatcca taaatgacat
    14461 ttcgcatttt tttacgatat tcttccggca tcatcccttt tgtctgagtt tgataagaca
    14521 aacatcgctg ggcattcatt cttccgtcac caagcaatgc ttttgtcgga caagcagtca
    14581 cgcagcgcat acagtcaccg caaccaaatt ctcctggttt atcaggctca aatacaatat
    14641 tagtaacgat ctcaccaaga tagacaaatg aaccgaactc ttcagtgatc aacaatccgt
    14701 ttcgtccaat aaatcctaaa ccagctcgct gtgctacagc gacatcgatc agttctccag
    14761 tatctacttg cggagctaga cgccattcct cttctttttg ccatttttct gctcttgttt
    14821 ggatgaattc gatcaatcgg ttcaaacggt ctctgagtat atcatggtaa tcgatccccc
    14881 aagaagcacg agcaaactgc ccgcgttttt catccctagg cacttcttca tggatcctag
    14941 taggatacgc aagtgcaatg gaaatgatcg atttcggctg atcaaatgtc atttctggat
    15001 aaagacgctc atcgatattt ggatgttcaa agccagaagt atggccagct gctttttgtt
    15061 cttccaacga ttcttttaat gaatcaaaag gttccgccgt ggtgaacccg attttgtcaa
    15121 ttcctaatcg tttgctttct tgtatgattt cttcttttaa tgtcatggtc ttcctcgctt
    15181 gctgttaacg gctgtataac ccataatata gtttttcttt tttcagctgg acaaactgga
    15241 tctgctgatc ggcaatcttc ttttcttcca tccatttttt tcttgcagca aatacttccc
    15301 aataatttgt tgaaattaat tttcttttgt tatctaatgc attaaaaaaa ttcaatgaaa
    15361 ctggttttgt acttaacagt tcatggagcc ttacctcttc ccatggcgaa aactcagaca
    15421 gatcgcggga atacaatact tcttcataga gatttttttg ctgtgtgtac gtgaatgttt
    15481 gcgggatttt cccaggccta gcaatttcta ctagacgatt ataaaacata ttcattaatt
    15541 tgctgttggc aataaattga cttttataat aggccgactg gatctttttg attactcctt
    15601 ctgctttgat gactgaaaat aatgcaggcc atgctttgac ggttatttcg tcgttttgtt
    15661 taactaatac gccaggtgtg aagccgactg gagaaaggaa cacaaaataa ccgataccgg
    15721 tattttcatc taaaggctga aattcatcaa acaaccattt gcctatgtac agctcttcat
    15781 ctggttctgt tgtctcattt tgatttgcta tgaataattt agctaattct gcttcaagaa
    15841 attgaaggtc ttgatccttg tcggtatttt cctgctggct gatcaatcga aagacttctt
    15901 ctttaatcgt atatttatct ttacgtgtaa aattcatagc cacaccttcc ttccttactc
    15961 taactataac ggaatttctt caaaaaaggt atctgtacga cgaaaaacag cataattttt
    16021 tttcatatct cttataaaaa atgcaaggtc tgctcctcaa gctagacctt gcacttttca
    16081 gctttctttt gtttgtgaca aaccttcatg gatcgtttca atattccatt tcatcatctg
    16141 atagtaagaa tctccttgtt ctccttcttt tgctatggaa tcagtgaaca gtttatcata
    16201 gattttcaaa cccgtttctt ttgctacccg ttccatgctt cttgagtcta cactggtttc
    16261 cacgaataaa accggtactt cttttgctct tatctgatca atgatcgctt tcatttgatc
    16321 aggcgtacct tgactttctg tattgatctc ccatatataa gctgctggca aatcatatgc
    16381 tcgtgaaaaa tacttgaatg ccccttcact tgttactaat aattttttgt tctcttcgat
    16441 agaagcaaaa ctttccttcg cttgtgtatc taattctttt agtttttcca catatgcttc
    16501 tgcatttttt ttatagagtg tcgcattttc tgcatctttt tcagagaata tacgtgcgat
    16561 ttcctccaca tattttattc cgttagatag gtctagccaa gcatggggat ctgcttttgt
    16621 atgttcttca ccgctagtta aataaagagg ttctacattt ttgctaactg caaaataatc
    16681 ttttccttct tcttttttag ccgtttccat caagttatcg aaccagctgt tacctgtttc
    16741 aagattcaaa ccgttgtata aaataacatc tgcatcgctt gcctttttga tgtcttctgg
    16801 tagtacttca tattcatgcg gatctgttcc gactggtacg atactgtgga tatctatttg
    16861 atctgttccg acctcttttg ccatgtccgc caagatcgaa ttggtagcca cgactgacaa
    16921 cttttcttta cttccttcat ccgcttggtc agaagcctgc tttccgcaac cggctaatac
    16981 caagaaacta actgccaaga ttaaaaagag tgattttttt gttttcatct atctgcttcc
    17041 tttctgaaat aaaattcctt ttgttggaga aaataaaaaa gcaaggataa aaaatgcggt
    17101 acatgctaac acgatcgtag ctcctgatgg ccaattgtaa ctataactga aaaacacacc
    17161 gacaactgca cttagcatac cgattcctgc agctgtaatg atcattttca gtaaatggtt
    17221 cgttagcaag taagccgtgg cggctggtgt gatcaacatg gcaatcacca agattgttcc
    17281 tactgtctgt aaactgacta cagccactaa tgtcaaaaag aacatcaaaa gataatggat
    17341 cagcgatgtg ttcagcccat aagcttttgc cattgtcgga tcgaatgatg tgatcttcaa
    17401 ttctttataa aagaaaaaga caaatatcaa gacgattcca ctaacgagtg ctgtcagcaa
    17461 caaatcgctt tccctaacag ctaagacatt cccaaataaa atatgataca aatctgtcgc
    17521 actctgggca tatgaaatca agataatccc taatgcaaaa aaggagctaa agacaattcc
    17581 aatcgctgta tcatttttca atcggctccg ctgagtcacg aaaccaatca ctgctgctgt
    17641 tagcatccca aaagcagtgg ctccaaagat gtaactaaaa ccaaacatat aagaaatggc
    17701 tacacctgga agaactgcgt gagagatagc gtctcccatc aaagacatcc ctcgtaaaat
    17761 aataaaagaa ccgatgactc cagaagcaag tcctacaagc atggaagtga ccaaagcgta
    17821 ttgcaggaat tgataattca ttaatccatc aataaaggat tggatcattt tttctctcct
    17881 ccttttacta aatgtctgat gatctcacca taggccaatt gaaggttctt cgatgtaaag
    17941 acttcttgta ctggaccgga agcgatcagc tgtttattta aaagaataac ctcatcgaaa
    18001 tattcttcta ctttatgaag atcatgatgg acgatcaaaa tcgtttttcc tgaattcttc
    18061 aattcctgca agatgtcaaa gatctttcgt tcacttaacg catcaatccc tacgaatggt
    18121 tcatctaaaa agatccattc tgctccttgg gctagagctc ttgcaataaa aactctctga
    18181 agctgtccac ccgatagttc gctgatctgt cttttggcat attcttccat ccctactttt
    18241 ttcaaagctt gtcttgcacg ttctttttct tttttcccag gtctttgtcc aattcgtaaa
    18301 gatggatatg ttcctaaaag tacaacgcct aatacatcaa ttggaaagga aagatccaat
    18361 tcacttcgct gttccacgta ggctattttt ttacggactg agtttaccgg ctgtccagaa
    18421 aaggtgactt taccagtagt agctggtatc aactctagca aagatttcat aaatgttgat
    18481 tttcctgcac cgtttggtcc gataataccg gtaatttttt gtggatttat cgtcaagttg
    18541 atagttttca aaacagtttt cccttgataa gcggctgaaa ggtcttgaat tacaatggcc
    18601 gtacgttcca aatcttacac tcctttttag gtttacctaa ctttttattt aaatatagtt
    18661 taactaaaat ggaaagcatt ttcaagtaga atgatgaaaa agtttttttc gaattttcct
    18721 cgcattttat gaaaattttc tgtagaatca catcaagagt tcaataaggc taacctaata
    18781 atgaaaaaca tgtgttgaaa cgtttgatta ttttttcttg ttgactcaac aacgaaagga
    18841 gtttttgtat ggaagaagaa aagttaactc gaagtcttag tgcccgtcat atccaaatga
    18901 tcgcactagg tggaacgatc ggtgtcggtc tgtttatggg tgcctcctca accatacgtt
    18961 ggacagggcc ctcagtgatg ggagcctatg cgattgcagg attctttttg tatttgatta
    19021 tgcgtgcttt aggcgaaatg ctttacgttg atccttcgac tggttctttt gcaaattacg
    19081 cgagcgagta catccatcct gtggctggct acttgaccgc ttggagtaat atttttcaat
    19141 atattgtcgt aggaataagt gaagtgattg ctgtaggatc ttatatgaat tattggtggc
    19201 ctgatttgcc ggcaattatt cccggaatca ttgtcgtctt gtttcttatg ctggcaaatc
    19261 ttatttctgt caaggcgttt ggcgaattag aattttggtt ttcgatcatc aaagtcataa
    19321 cgatcatttt gatgatcatt gcaggtcttg gggtaatctt atttggattt ggtaatcatg
    19381 gtcaggcagt agggatttca aatctttgga aaaatggtgg attcttcact ggaggcgtca
    19441 aaggattttt ctttgcatta tcgatcgttg ttgcttccta tcaaggaatc gaactgatcg
    19501 gaatgactgc tggtgaagca gagaatccta aaaagacgat cattgaagct gtccagtcca
    19561 ctatcggtcg aatcttgatt ttttatattg gtgctatttt tatcattgtg agtatctatc
    19621 cgtggaatca attgagtgag attggttcac cttttgtaca aactttttct aaagtcggca
    19681 ttacatttgc tgcaggactg attaattttg tcgtgattac agcggcatta tcaggttgca
    19741 attcagggat tttcagcgca agtcgtatgg tctatacact tgccataaat ggaaagatgt
    19801 ctaaaaaatt tctgaaacta tctcgtcacg gtgcgccttt ttatcctgtc gtagcaatct
    19861 caggtggaat cttaattggt ctgatcttaa attatcttct accaatgttc atcaaccagt
    19921 cagaaaatat tttcgttttc gtatacagct caagtatttt gcctggtatg gttccttggg
    19981 tagtgatttt gatcagcgag attcgcttca aaaagaagca cgcagataaa atgcatgatc
    20041 atccttttaa aatgccacta tctccttata cgaattatgt gacacttgct tttttagcta
    20101 tcgtattgat tttcatgttt atcaatcctg aaacgagaat ctcattgatt attggactcg
    20161 ttttcttgct ttacatgacg atccattact ttgtacgcga aaaaagaacc ggaaaataaa
    20221 aaaagaacga agcttcacct tagtggaagc ttcgttcttt tttattgctt ttatcaattc
    20281 aattttagcg tctcaccggg agtcaggaaa taattctcca tgctcatccc attcagcgac
    20341 aagatcgttt ctactggtac gccagttcgt tcggctattt gatttggacc ttctccttcc
    20401 aaaactacgg ttgtcgcagc ctgtgaagtc gcgctgttgg tattagtttc agaggaatct
    20461 gttgtgtctt gtgttgttcg agcttgcatt tcagaagctt ctaccgactc tgctgtctca
    20521 gaatcagaag atgttgtgtc ttgtgtgctt gttgactctg cttgaagaga ggcgctagtc
    20581 tgcaaataaa tatcggaaat cttagcgaca tgattcgtat aattagccga taattgattg
    20641 atcaaagtat ctaagtctac accgtcttta ttttctgctg cctgatacgc tgcacgtaat
    20701 tttctgatcc cttcgtcgga gattgcttga agctcacgtg ctttttggtt agcaactgct
    20761 gataaaccag ctattccctc ttgatttccc tgaatttctg tctggtattc ttcaatcaat
    20821 gcaggcgtct tttcttcgat ctttttcgaa taatcattta cgattgcttg aagttgctct
    20881 ggcaaaccat ttgctgtttt ttcttcagaa gtttgtgtct cttttgtttt agttgatgac
    20941 tcacttgttt gtttcgtctc tgttttcttt gtcgttgttt ccgttatggt cgtacttttc
    21001 gcttctgacg aattttttcc atgaaaatat agacccgcaa ctcctcctgc tatgatcaaa
    21061 aacacggcaa ctgctgcccc aatcatgatc atccgcttac gagctgcctg tttttgctgt
    21121 cttcttcggg tcatccgaac attttctccc cactcatttt catcatacat ttttttcacc
    21181 accaattttt atgtataata gcgttatcaa acgcttcata ttcttattgt agcaaataat
    21241 tgtcgctgtg cacattttta tctttgttat ttataagaaa atttcaccag ttgagatgat
    21301 tccaagattg tccgtcgatt tctccttgca gctgcattgt tcgttcgtct atctcagcca
    21361 tcagtttctt tatttcatat aagagcgcac ggtcttgata ttctgatttt tcgcttaatt
    21421 catctaattg ttggatcagc caatcatttg tcataatgaa gttcctttca tttcttgcag
    21481 tgtaaaggtc aagtcttcga tctgctcttt tgcctcagtc agtgcttcta cgatatatgc
    21541 ttgtttttca gattcttctt cctgcatttt ctgattttcc tgtactcttt gaataaacca
    21601 gtcaatccgc tgagtcagcg atggtttgcc ttccaatgag agatactgct gataagcaat
    21661 tagcttctcc caacgttttt cctgggataa atacataaat tgtgctaaaa cgaatggcgg
    21721 aagataggtc atatggaatt tatttgcgat tcgctgatac ggcctcaaaa attcggaaat
    21781 cgtatattct tcttttcctc cagcttggta ttctttttcc gccacacctg ttgtcaatac
    21841 gattcctagt tctttccctt ttaatacatg tgctgctcgt tccaagacat tatcttgcca
    21901 aatttttaaa tgagcaggag aactatacca gtaaaacgga aactggaaaa tgatccgatc
    21961 atgctgggcc aagagctgta gttcagtctc tatatctatt tttccgtctg gataacttga
    22021 ttccaaatga tgccaagtag ctggtaaacc agcagaagct tctttgaaaa attgctgatt
    22081 taaagattct tcgatcgttg gatggctaat gatgataagt gtacgcatcc cttttcctcc
    22141 tttttagtct tcgctttaca gtgtagcata accgccacaa gtttcttttg ccgatgcttg
    22201 tcattttaac cagactaatc aggttgtcca agaaactgcg gtttgttttt ccaactgttt
    22261 tttcaaggta tccaaagcaa tattcttaaa attttcgttt ttcatacgtt tacgatagtt
    22321 cgtagccgtt tctccggtac tctttttgaa cattcggcta aatacgacta gactattgaa
    22381 ccctacttga taggcaatac tttccacgga ttcatccgtc agagaaagaa gaaacgcccc
    22441 tcgattgatc cgtaccccga tcaaatattc cttgatactg gtacccatat gatttttgaa
    22501 tactcgcgac aaatggctcc gattgaccgc taaatgctgg gcgatttcct caacagacaa
    22561 tttttcagca taatgtgcct cgatgtattt gactgtttca acagctaaag aggaatgaac
    22621 cttttgtaca ccaccgctga cctttccgcc atcttttaaa aggactgata aaaaacggta
    22681 ggttaatgct gttaactgta attcattttc caaggtttct tccgtatctt tcatgcattc
    22741 taaaataatg tcgatatatt tttgtatgtc agaagagacc atcgtgtatt ggtcttctct
    22801 gaaaagcgac tgccgaatga tttcttctgc agcactccca ccaaatgaaa gccagcaata
    22861 catccacgga tcttctccat ctgataagta aaacgtcgag tctcctggac gaatcaagaa
    22921 cagatcacct tttttcaatt ggaattgttg atttttcaca tgataagatc ctttgccttc
    22981 catcacgaca tgcaggatgt aatcattgcg aatagcagga ccaaaagaat gaaacggcat
    23041 cgttttagag aaaccacaaa aatcaaaata caattctttt tctacttgtt gatgttcctt
    23101 ttttaaaaaa atcgattgct ccacgtctat ccctccttac tcctttagta taatgcaaca
    23161 aaaagttaaa taaaagtcac agaaatatat taaaaaggaa gcgatttctt gttaagttta
    23221 agtcattaaa agatctttgt tattcgttgt aaccggttta cttagtatta tatattgaac
    23281 aggagaggaa tacagattat gaaaagcaaa atgatccaac gtgtgtccta cgctttaggc
    23341 gcttttggac acgacacatt ctatgttaca ttaagcactt attttatggt attcgtgaca
    23401 agccagatgt tcgcaggaac agataaggca acgaatgcaa aaatgattgc tacagttaca
    23461 agcttagttg taggtatccg cttagttgaa attatttttg atcctttgat tggtggcatc
    23521 atcgataata ctcgtacaaa atacggtaaa ttcaaaccat ggttagttat cggcggcgtt
    23581 gttagttccg tactcttggt agcaatcttt accaatttct ttggcttagc tactcataat
    23641 acgactttat ttatcatatt atttgcaatc gttttcatct tattggatag tttttattca
    23701 tttaaggata tcgctttttg gtcaatgatt ccagctttga gtacggaaac aaaggaacgt
    23761 gaaaaatttg ctacgtttgc tcgtttcggc tcttctctag gtgcaaatgg tactactcta
    23821 gtcgttgtcc ctatcgttac tttctttacc tatcttgtta ctcataaaca tattgaaggc
    23881 gcttcaggct ggttctggtt cggtgtgatt gctgctgtta tttcaggcgg tactgcgatt
    23941 atcactgcct tgggcacaaa agaagtcgat actgcgatcc gtcagcaaaa tgaaaaagct
    24001 gggatcaaag atgtgttcaa agcagttgct caaaatgatc agttgatgtg gcttgccctt
    24061 gcttatatcg cttatgcgat tgccaatgta gcaacaactg ccgtattatt ctacttcttc
    24121 aaatatgtac ttggccaacc aactgaattt tggatcgttg gtctagttgc tgccttactt
    24181 ggtttagtag ctgttccact attcccaatg cttacacgcg tcatctctcg tcgctacgtt
    24241 taccttggag ggatcgcaat gatgatttct tcttactttt tctttacgat cgcaggttca
    24301 agtttgccaa tcgttatcat cgggctagta ttgttctact tccctcaaca attgattttc
    24361 ttatccgctt tgatgacgat cacagactct gttgaatatg gtcaatggaa aaatggtatc
    24421 cgtaacgaag ctgttactct atcaattcgc cctttactag ataaaatcgc tggtgctttg
    24481 tctaacggga tcgtagggtt tgtcgctatc gcttctggga tgactggtaa tgctactgct
    24541 gctgatatca ctgctcatgg cgtaacgact ttcaaagctt atgcattcta tgctcctgct
    24601 gctttgatga ttttctccgc tattattttt gcttggaaga ttacattgac agagaaaaaa
    24661 catgcacaaa tcgttgagga attggaaaac aaattggctc cagctgatac aatggaagat
    24721 gaacttcacg atgcagtagt tccttcccat taagaaagga tcttgaaaat gaagattaca
    24781 gaaaaagatt ttggccaagg ttatcacttg ataaccttgg agaacaaaaa caagctagca
    24841 ctttctatct ctgatttagg tgctagaatc gtcagtttaa aatcaaatga tcgagaatta
    24901 gttttgggat ttgacaccgc tgaagaatat atcgaaaaag atccgtatat cggtgcttct
    24961 atcggtcgca ccgcagggag aatcgaaaat ggccgctttt ctctcaatgg aaaaacttac
    25021 caattagcca cagatccaaa aactggacat aatctgcatg gtggtgctcc tggatttgaa
    25081 ttaaaaaaat ggtcttatgt cattttgaat ggcgaaaatg aagcttctgt catttttact
    25141 actacaagtc ccgacggaga acatggtttt ccaggtacaa tggatgtgga aatccgctat
    25201 actttgacaa aagataatat ctggcgagtg acctctcgcg gcaccagtga ccaagatact
    25261 ttattcaatc cgacaaatca tgtttacttc aacttgacgg gagatgcttc tcagtcaatc
    25321 gatcaacatg agctttggct caatagcgaa gcttatgctc ctttgcgaac agatagtatt
    25381 cctatagggg taaaagaaaa tgctgcagga agtgctttcg attttcaaat acctaaaaaa
    25441 ttagcgtccg tttttgctag tgatttggat caaaaaaatc ttgttgacgg aatcgatcat
    25501 ccatttttct taaaagaaac cggcttgggt aaagaagccg ctcgcttgac tagccccgat
    25561 aaaagaatcc aagtaggtat tgctacagat gcttctagtg tggttatttt cactgcaaat
    25621 ttcggcacag aaacaccaga aatgcgcaac cgaaaactgg cacatcacgg tggcatcaca
    25681 tttgagactc aaacagctcc aggtgcagag cgcttctcag catttggttc gattcattta
    25741 aaagctggat cagtatttga aacagtgaca gaattcaaaa tcaaaacaag aaaggaataa
    25801 ctcatcatgg aaatcaaaag aagcttaaac acaaaatttg ccgaaacatt cggacctaaa
    25861 aaaactacac aatacttctc accaggacgc atcaatttga ttggtgaaca cacagattac
    25921 aatggaggcc atgtcttccc cgcttccatc acttatggaa cgcaaggtgt agcagcacct
    25981 cgagacgata ataaaatatt agtttattcc acaaactttg acgatatcgg tgtcattagt
    26041 tttactttag atgagctaga atatgataaa aaagcaaact gggcaaatta cgtaaaagga
    26101 atggtcctta aattaaaaga agctggacac tcaatcgatc atgggtttga gttgttagtg
    26161 gaaggaacaa tccctaatgg tgcaggactt tccagtagcg cttcattaga attattagta
    26221 ggtgttgtat tagaagatct gtttgattta aaaacagatc gcttggaact agtccaaaca
    26281 ggtaaaaaag tagaaaatga attcatcggt gtcaattcag gtatcatgga tcagtttgcc
    26341 atcggcttcg gtgagctaga tcatgcaatc ttattagata caaatacgct gaaatacgaa
    26401 atggttccag tgaaactaga tggttatgca atcgtcatca tgaatacgaa taagcggcgg
    26461 gaattggcag attcaaaata caatgaacgc cgtagcgaat gcgaagaagc cttggcacgt
    26521 ttgcaaaaaa ctttgcctat ctcttcttta ggagacttag atgaagaaga atttgaatca
    26581 actattgacc aaatcggcga tgatacattg atacgcagag ctcgccatgc cgtttctgaa
    26641 aatcagcgga ctttgaaggc aaaagctgaa ttagaagctg ggaacttaga agcatttggt
    26701 caactgttaa atgattctca ccactctctt cgctatgatt atgaagtaac tgggatcgaa
    26761 ttagatacac tagtagatgc tgctcaaaaa caagaaggtg tcttaggtgc acgaatgaca
    26821 ggagctggat ttggtggctg tgcgattgct cttgtcaaag aagaaaatat acctgccttc
    26881 aaaaacaatg tctatgatga atacatgaaa gttgtcggct atgctccgga attttatgtt
    26941 gcacacattg gaaatgggac aacaaagatc gatccagaag ctgttcatgt ttgataaaaa
    27001 aatcaacgat ctcagctaaa aacaaataga taaaaataac ctctcagcaa tttccaaatg
    27061 tgagcctttt gctcgtattt gaaaattgct gttttttatt gacacggaaa gagaacgtgt
    27121 gttctaattg tgttgaagag gtgattgtta tgtgggcgaa tcaaaatccg gtatttgttt
    27181 atgaaaaaga accttcaaga gatattttat gtattgactg caaatctttt tacgcctccg
    27241 tagagtgcgt tgaaaaggat cttgatcccc ttacaactaa attagtagtc atgtcctatc
    27301 cgtccgattc gacagaaaca cggggtagcg gattaatctt ggcttccagt cctgcagcaa
    27361 aaaaagctta cggcatcacc aatatcagcc gtgccagaga tcttcccttc ccctatccaa
    27421 gtgatttata tattgttgcc ccaaggatgg cctattatat ggaaaagaac agagaaatca
    27481 attctatcta taaaaaatat gcagacgaag cgaaccatca tgtctattcc gtcgatgaaa
    27541 gcttccttga tgtaacaaat agcatggcac tctttggtaa aacaaatgcg tacgacatgg
    27601 ctaaactgat ccataaagat gtgtttgagc agaccggaat ctacacgact ataggaattg
    27661 gcgataaccc gctacttgct aaactagcct tagacaatgg ctcaaaagac ggaaagcaaa
    27721 tgtttgctga atggcgttat gaagatgtac cagaaaagtt atggaaaatc tcagatatca
    27781 ctgacttttg gggaatcggc aaacgaacag caaaacgtct gaaacaaatg ggtatacatt
    27841 ctgtttatga tttagctcac gcggattatt atcacttgaa aagcaagcta ggggtcatcg
    27901 gtactcagct ttatgcccat gcttggggca tcgatcgaag ttttttggga caagaatata
    27961 caccaaaatc aaaaagtatc ggaaatagtc aaatcctaga tagagattat acaagacgag
    28021 aagaaatcga gatcgttatc aaagaaatgg ccgaccaagt cggcactcgc ttgagacgat
    28081 ctgcagcaaa aacacaagta gtcagcttat gggtaggata ctctttaggc tatcttgatc
    28141 caaatggcaa gaccgggttt caccagcaaa tgaaaatcga agcgacaaat gctagcagag
    28201 atctagcaga tgctttattg accattttcc accagcatta ccactaccag gatattcggc
    28261 gtgtaggcgt gaactgttcc gacctcgtat acgcgacagg tttacagctt aacctttttg
    28321 aagatcctga aaagcagata aatgaagcga aagttgattt tgttgtcgat acgattagaa
    28381 aaaaatacgg ctttcaatca atcatccatg cgcatagtct cttgcaagga ggacgcgcca
    28441 tcgcacgtag ttctctcgtt ggcgggcatg caggtggtat gagtggatta gaaggtggaa
    28501 aagatgagac ggacagcaaa aaaattcatt gactataacg agtatcgaga tcggccattt
    28561 ggattaaaat gggggactgc ttttgcgatg gaagaactag tcaacggtat caaaaaaaat
    28621 gaagaagaag cacgcaaaca gcctcttcct aaacaacaaa tgacattgca agaaatcgat
    28681 agtatactga cagaagcctt tttatctcgt aaagcagtaa tcattcagct caatttaaaa
    28741 gatgaatttg gaagactctt ggatgaggtc gaagggcact tcattggtga agcctatgaa
    28801 gattacttta tgattgacca tcagccgata ttgtgggaag acgtacggta tattgagtta
    28861 aaggaaacaa aaaaatggtt tgtgacatga gaggttgtga cagaagtgat caacttcaaa
    28921 aaataagaaa ggatttttcg aaaattgctt cttaaatttt tgtggaattt tggcttattt
    28981 ttcgaagaag caacttctgg aacaacgtat attagatatc agaggttgtg aaaaagtcgc
    29041 ttagttttga gtaacaagcc aaggcgctga tttcacaacc tctgtctatc tatccatcta
    29101 ttcggtaaga ttcatgcatg acgtcttctt tatttagtat gtagagccct ttttcttctg
    29161 accattcaac ataaatcagc tgttttacat ctgcatagcc ttctttcttc aagttctcca
    29221 taagccatgc ttcagtcaat tgattggact gaagcacttt atgctgaatt tgcccttcat
    29281 ctacaagcag gatcgataaa gctttgtctt catctgattt tttcaataca ctcatttgac
    29341 cagcattctc taggataaca tgtttggcat tttcaagaga aaagcattct tgctgtctta
    29401 agattgctcg caactgctcc atctcgatat ggttatttgt caactctgtc atgttgatga
    29461 cgccgtcttt gattagaaca gatggctcgc ctttgaccca acgattgacc ttttcatttt
    29521 tttggacgat acgttcgata ccataaatca ttactgccca aagcgcaatg gcaaatagga
    29581 catgccctac atggacattg tcatcatagg ttgattcttc taagatccca cctaaaacaa
    29641 gcgtatagac taaatcaaac ggtgtgattt ctgacatgga tttcttccca agcaaacgta
    29701 ccactaataa taaaccgatc aacccgaaca ccagtttcat tgtaattgaa aaaatcattt
    29761 tttctccccc taactatctg ttaattaaat tatatatgct ttctcacgaa cacgcatgga
    29821 atatacaagg aagaaatgta tccttttact aaaaaaagga ctgtgacact gtagtgaccc
    29881 ccaaaagtta gacttttttg gagtggagaa atccactcct tttttttatg ctctaaaatg
    29941 atatttttac acaaccgtta cggttgtttt acgaaattgg acg
//
LOCUS       DAZKBH010000022        29336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000022 DAZKBH010000000
VERSION     DAZKBH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.022"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..940)
                     /locus_tag="ACG8OO_000980"
     CDS             complement(92..940)
                     /locus_tag="ACG8OO_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9044903.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1164..3104)
                     /gene="thrS"
                     /locus_tag="ACG8OO_000981"
     CDS             complement(1164..3104)
                     /gene="thrS"
                     /locus_tag="ACG8OO_000981"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9044904.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3441..3620)
                     /locus_tag="ACG8OO_000982"
     CDS             complement(3441..3620)
                     /locus_tag="ACG8OO_000982"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFP9044905.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3667..5490)
                     /locus_tag="ACG8OO_000983"
     CDS             complement(3667..5490)
                     /locus_tag="ACG8OO_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9044906.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5768..8047
                     /gene="helD"
                     /locus_tag="ACG8OO_000984"
     CDS             5768..8047
                     /gene="helD"
                     /locus_tag="ACG8OO_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFP9044907.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8140..8787)
                     /locus_tag="ACG8OO_000985"
     CDS             complement(8140..8787)
                     /locus_tag="ACG8OO_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044908.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8966..9685)
                     /locus_tag="ACG8OO_000986"
     CDS             complement(8966..9685)
                     /locus_tag="ACG8OO_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9044909.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9816..10967)
                     /locus_tag="ACG8OO_000987"
     CDS             complement(9816..10967)
                     /locus_tag="ACG8OO_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFP9044910.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11184..11924)
                     /locus_tag="ACG8OO_000988"
     CDS             complement(11184..11924)
                     /locus_tag="ACG8OO_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9044911.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11928..12251)
                     /gene="rsfS"
                     /locus_tag="ACG8OO_000989"
     CDS             complement(11928..12251)
                     /gene="rsfS"
                     /locus_tag="ACG8OO_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9044912.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12308..12901)
                     /gene="yqeK"
                     /locus_tag="ACG8OO_000990"
     CDS             complement(12308..12901)
                     /gene="yqeK"
                     /locus_tag="ACG8OO_000990"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFP9044913.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12885..13535)
                     /locus_tag="ACG8OO_000991"
     CDS             complement(12885..13535)
                     /locus_tag="ACG8OO_000991"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9044914.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13545..13856)
                     /gene="yhbY"
                     /locus_tag="ACG8OO_000992"
     CDS             complement(13545..13856)
                     /gene="yhbY"
                     /locus_tag="ACG8OO_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9044915.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13872..14981)
                     /gene="yqeH"
                     /locus_tag="ACG8OO_000993"
     CDS             complement(13872..14981)
                     /gene="yqeH"
                     /locus_tag="ACG8OO_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFP9044916.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14978..15505)
                     /locus_tag="ACG8OO_000994"
     CDS             complement(14978..15505)
                     /locus_tag="ACG8OO_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFP9044917.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15685..16470)
                     /locus_tag="ACG8OO_000995"
     CDS             complement(15685..16470)
                     /locus_tag="ACG8OO_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9044918.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16463..17332)
                     /gene="accD"
                     /locus_tag="ACG8OO_000996"
     CDS             complement(16463..17332)
                     /gene="accD"
                     /locus_tag="ACG8OO_000996"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9044919.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17333..18712)
                     /locus_tag="ACG8OO_000997"
     CDS             complement(17333..18712)
                     /locus_tag="ACG8OO_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9044920.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18720..19139)
                     /gene="fabZ"
                     /locus_tag="ACG8OO_000998"
     CDS             complement(18720..19139)
                     /gene="fabZ"
                     /locus_tag="ACG8OO_000998"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9044921.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19164..19640)
                     /gene="accB"
                     /locus_tag="ACG8OO_000999"
     CDS             complement(19164..19640)
                     /gene="accB"
                     /locus_tag="ACG8OO_000999"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9044922.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19643..20878)
                     /gene="fabF"
                     /locus_tag="ACG8OO_001000"
     CDS             complement(19643..20878)
                     /gene="fabF"
                     /locus_tag="ACG8OO_001000"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9044923.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20897..21634)
                     /gene="fabG"
                     /locus_tag="ACG8OO_001001"
     CDS             complement(20897..21634)
                     /gene="fabG"
                     /locus_tag="ACG8OO_001001"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFP9044924.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21646..22563)
                     /gene="fabD"
                     /locus_tag="ACG8OO_001002"
     CDS             complement(21646..22563)
                     /gene="fabD"
                     /locus_tag="ACG8OO_001002"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9044925.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22670..22894)
                     /locus_tag="ACG8OO_001003"
     CDS             complement(22670..22894)
                     /locus_tag="ACG8OO_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9044926.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22947..23909)
                     /locus_tag="ACG8OO_001004"
     CDS             complement(22947..23909)
                     /locus_tag="ACG8OO_001004"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9044927.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23919..24359)
                     /locus_tag="ACG8OO_001005"
     CDS             complement(23919..24359)
                     /locus_tag="ACG8OO_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9044928.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24665..25630
                     /locus_tag="ACG8OO_001006"
     CDS             24665..25630
                     /locus_tag="ACG8OO_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9044929.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25687..25917)
                     /locus_tag="ACG8OO_001007"
     CDS             complement(25687..25917)
                     /locus_tag="ACG8OO_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFP9044930.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26003..26890)
                     /locus_tag="ACG8OO_001008"
     CDS             complement(26003..26890)
                     /locus_tag="ACG8OO_001008"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9044931.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26979..28202)
                     /locus_tag="ACG8OO_001009"
     CDS             complement(26979..28202)
                     /locus_tag="ACG8OO_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9044932.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28219..29265)
                     /locus_tag="ACG8OO_001010"
     CDS             complement(28219..29265)
                     /locus_tag="ACG8OO_001010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9044933.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8129 a         6423 c         5058 g         9726 t
ORIGIN      
        1 cttaagtcgt gaaacacttt gatggatcat ttatttgtgg aaaactacgt tttgttattc
       61 taaaaaactt atataaaaaa tagtaaggtt tttatttcga atccataaaa tattcttgga
      121 ttgcttctcg tgtttttttc actttttcgc taagcttttc gtttttagcc atatagaaat
      181 aaaaaatgat atcgatcgta gcaaattggg ccaaaagtga attagttgcc gcacttcgat
      241 tcgtgatttc ttttggtcta gatgtttgta cgagtacatc ggctatcttg ctaagcgggt
      301 tatttccaaa ttgcgtcaac gcaatgattt cgatattcat gcttttcgcc agctcggcta
      361 atgcgactac atctgcactt ctacctgagt ttgaaacaag ccaaagcact ttctttttct
      421 cattgctgac aagttgcggg agcaagacgt ggatatcttt ttcaaaaatg attggcttac
      481 cgacacgaga ccatttttgc agaatatctt ctactactaa agaagaggcg ccaaccccat
      541 ataaaaaaac agtctctgcc gattccaaac actctacgac tttttggatc gtttcttctt
      601 ctagaatatc tactgtttct tttatcgcta cttgtgcatt gttaccaagt ttgtttttga
      661 tagagtaaaa ggactcattt gcttctatat cactaaaacc ttcatcgatt ccagtctgta
      721 gttctgttga aatcgtgatt ttgaagtccg tcagactttg aaaaccgatt tttttggaaa
      781 aacgtacaac tgtcggcgca ctcaacccgg aagcttctgc taattcatta gctgtcattg
      841 tagggacctt ctcaatatta ccgataatgt agttggccaa ctttttttct gctgaagaat
      901 aatcttccat catactttca atacgactaa ttacgctcat aagttcctcc acccttttaa
      961 tgatagtcta tgtaagtata ccatatcaga cccttattca cggtcgtata ctggataaaa
     1021 aacaaaaaaa caatccgccc aacagaatat cttatcccat caatcccaca gattctattg
     1081 agcatctgtg tctttgagga atacctgtta tctattgttc ggattgcttt ctatctacta
     1141 cttttttaca ggaaatttac ctattagttt tctctgctgt aatttttgac ttctgcttcc
     1201 atagcatcaa taaagatatt caaatcttct acagctgttt ctttacttcc ataacgacgt
     1261 acgttcactg ttgcatcttc caattccttg tctccaacaa ctaattggta agggatcttt
     1321 tgtgtttgag aggcacgtat cttatagccc attttttcat tacgatcatc aacttctaca
     1381 cgcaacccct tcatttgcaa acgttctttg atttcataag cataatcgcc atgtgcatca
     1441 acagaaacag ggatgattgt tgcttgtata ggtgccaacc atgttggaaa agcgcctttg
     1501 tatacttctg ttaaataagc gacaaaacgt tccattgtag aaacaattcc gcggtggata
     1561 actactggac gatgcgtatt ttccccgtct tctccaacat atgtcaaatc aaagcgctct
     1621 ggcaataaga agtctaattg tatagttgat aatgtttctt ctgtaccaag agctgttttg
     1681 acttgaacat ctagttttgg tccgtaaaag gctgcctcgc cttctgcttc aaagtaatct
     1741 agttctaatt catccatagc ggctttcagc attgcttggg ctttttccca cattgcgtca
     1801 tcatcaaaat atttatctgt attgtttgga tcgcgatagc tcaagcggaa acgataatct
     1861 gtaatattga agtcagcata tactgccacc atcaattcaa gcgtacgttt aaattcatct
     1921 ttgatttgat caggacgaac aaatgtatga ccatcattca acgtcatttc tcgtacacgt
     1981 tgtaaacctg acaaagcacc tgatttttca tagcgatgca tcattcctaa ttcagcgata
     2041 cgaattggta attcacgata agaatggatc gtgtttttgt agaccatcat gtgatgaggg
     2101 cagttcattg ggcgaagaac aagcatttcg ccatctccca tatccattgg cgggaacatg
     2161 tcttcttgat aatgatccca gtgtccagat gttttgtaca actcaacatc tcccattatt
     2221 ggtgtatata catgttggta acctagacta acttctttgt ctacgatata acgttcgatc
     2281 gtgcgtcgga ttgttgcccc ttttggaagc cagaatggca aaccagaacc tacttctggg
     2341 gaaaccatga acaagtcaag ttcttttcct agtttacgat ggtctctttc ttttgcttct
     2401 tcacggagac ggatatattc tttcaaagct ttcttgtcaa aaaaggctgt cccgtataca
     2461 cgttgcatca tttgattgtt tgagtttcca cgccaataag cacctgccac tgataataat
     2521 ttaaatactt gtatacggcc agtggatgga acatgtggtc cacgacacaa atcgatgaat
     2581 tcgccttgtt gataagcagt aatcacttct tcttctggca attcactaat cagttctact
     2641 ttgtacggat cacttgcaaa aatttctaat gcttcctgtt ttgaaagaac acggcgttcg
     2701 atcggcagat tttctttgac gattttcatc atttctgctt cgattgccgg caaatcttct
     2761 gcagttactt ggttttgtcc attatctgta tcgtagtaaa accctgaatc gatagctggt
     2821 ccaacgccaa agtggatatt tggatagagg cgacgcatgg cttgggccat caaatgagct
     2881 gctgaatgac ggactaagcc aagtgcatct tcatggtctg gtgtgatgat ctcaatactt
     2941 gcatcttcct cgattggacg agtcaaatca acgagttttc cgttcacttt accagctaaa
     3001 gctttttttg ctaagctttt tgaaatgctt tcagcaattg ccaatgttgt tgttccagac
     3061 tcaaattctt tgactgcacc atctggaaaa gtaattttta tcataatagt ttcctccttg
     3121 aaatgttttg attgatcaga tcatcaagct gaaaaacaaa aaaatcctag catctcttga
     3181 aaagagatac taggacgaaa agttcgtggt tccacctaat gttcaaacgc aaatgcgttc
     3241 ctcaagcgtt gtaacggtcc gaccgccctt gcttcaacaa gggtttcgaa agtggtcatc
     3301 ctttggtttt ctcctggaaa agtttcagcc tagcttttcc tctcttatcg aagactctca
     3361 aattcagttg gctttctcat caatcaatta tgtgtttatt taaccactaa taaatagaaa
     3421 aatcaagtgg gaaactaaac ttattttttc cattccccat ttacaccgta tgttcctttt
     3481 ttcatctcat tgatgatgac atggatgtgt tcttttggtg cctgcgtgtt tttatgaaca
     3541 gcttccgtga tatctttcat catattttct aattgttccg gactgcgacc ttctactaat
     3601 tcgacatgta cgaatggcat aatgctctct cctctttctg atcgttcttt tttcaactcg
     3661 attctatcat actatgtttg tcttgggtac tgaatcagga aaaatggctg gatctttttt
     3721 tgctctcaag gcactcaaaa tagagaccgt atccacgact tcttgcaaaa cagctccgaa
     3781 taatgcgggg attagtcccg tacttgcaat taacatcaaa aaaatacaaa taaagatgcc
     3841 aatcagtaca gactgtttcg ctattctcat cgtatctctt gaaatcacta ctgcttttgc
     3901 tacacgcaag agatcatctt tcaagatgac cgcatctgca ctttcactag cagctgtaga
     3961 accatgagcc cccattgcaa taccgatatc tgcagcagca agtgctggtg cgtcattcac
     4021 gccgtctcct accatcgcaa caggtctttg atctttaggg atactttcta aaatggtcag
     4081 tttatcttga ggcaagctac gggcatgtac agaatctaat tggacttttt ctgcaatcgt
     4141 ttgagcaatg gcagatccat ccccggtgat catcatcaaa tttttcacat caagttttct
     4201 tagctccttg attgtctttg ctgcttctgg gcgaatctga tccgaaaata ggatcgttcc
     4261 tgcaaaagat cgatcaattg ttacatagat tgctgtattg gacgtttcta tttcttcagc
     4321 tgaaacaaaa gaggcacgtc caatctttac ttcatgatta ttgactactg cttggattcc
     4381 ttgaccggtc acttctttca gttgactaac aggatacagt tggctgtttt tcacgttgtt
     4441 cactaacgaa cgagctaaga tatggctaga ttcctgttct gcactagctg catacagaag
     4501 tagctcttct ttttttattc cattgactgg ctgaatcgcc tctacttcca atatcccttt
     4561 ggtaagcgtt cctgttttat caaaagcaat cgtttttgta cgtgcgagct tttcaatcgt
     4621 tgttccagtt tttacaacga tcccgttttt acttgaacgg ctcattcctg caactaaagc
     4681 aatgggcgca gctagaatca gtggacaagg tgatgccact accagtacct cagcaaatcg
     4741 taaagggtct tttgagaacc accaagaaat accaccgatc aaataagcaa tcaacgtgaa
     4801 agggactgca taacgatcag ctaaacggac aaatcgggca ggcttttctt ttgattcttc
     4861 gactagttta ataatcattt gatactggct gtctgcggca cgtttttcag cgatcatttg
     4921 gatcgatccg tctccattta aagaccctga cataatctga tctccaggtt tcttggtttc
     4981 cgggcgggac tcacctgtca aagaagattc atcgaccgaa gattctcctt tggtcactcg
     5041 accatctgcg ggtacaagtt cgccaggctt gactagtaac tggtcatcaa tatttatttc
     5101 atcgattact atatcctgca attttccttc tttgaatcga tgggcaattc ttggggtgtt
     5161 atctaacaaa gactgcagtt ctttacccgc tcgtttattc gcgtaatcct ctagactatc
     5221 tccgcccgta agcatgatca atatcatcaa actagcccag tattctttga cggctaatgt
     5281 tgcaaggatt gcggtaatcg ctaatacatc tacgccatat cttcctgaac gtaacgtctt
     5341 gatcatttcc aaaaacatca agaaggcgag aatccctcca gtaaatgcga caatcgcaaa
     5401 tgcaagggcc ggctggtgaa aaacaaattc acttaataaa gctatcccac caataagcaa
     5461 agtaagtaaa aattttttga aatgtgtcat tgttttttct cctcttttat gtgacttaga
     5521 gaggttagta ataaatttcc agctgcctct ttcatcttaa gcatagtcct tgactagcac
     5581 tttttaaaac aataggcctt aattgaaaac aactatcact atcagcgttt ccaaaattca
     5641 tcagttctac aaacaaaaat attcagcaag gattactttt tcgattcatc agttaaaaag
     5701 tacctttttt cgaaaaacta tgctatactt ttaattcgtt atttacggcg gaaggagcaa
     5761 acatgcgatg aatgagaaac aactagaaca aacgcacgtg gatgaaacga tcgaactaat
     5821 ccagatcgaa caaaatatgc tggatacaaa acaacaagaa ttgactcagg aaatgaatca
     5881 ttcgacaaaa gacacagcta atcacaagat tcgagctggt tccaatgaag ctttttatga
     5941 atctgctgtt gaatatcgcc aacatgagca ggaattgctt ttgaaatacc atactcttga
     6001 agcacagcaa aaacgcctgc aaacgttgtc tgtcatgaaa gatagtcctt attttgcccg
     6061 tatcgacttt aaagaagacc gtgaaacaga aacattatat ctaggtatcg cttctttaag
     6121 agatctggca gaagaaacaa tcgttattga ctggcgcgca ccaattgcca atctttatta
     6181 tgaaggtgag attggtcccg cattttatga aactgaaaca gatagaatag atgtcgatct
     6241 gcagttgaaa cgacaattta aaatccaaga aggacgtatt ttatctatgg tagatacgtc
     6301 ggaagtaatc aatgatgact ttttgctaga gatattggat gaatcatcaa gcaaccatat
     6361 gaaaaacatc gtctctacga tacaaaaagc acaaaatgca atcattcggg acacaacaag
     6421 taaagtaatg ctgattgaag gaatcgccgg aagtggaaag acatccgctt tgcttcaacg
     6481 tattgctttt ctgttgtatc ataatcgcaa gtggctcgag gctgaaaacg ttcttctatt
     6541 ttcacccaac catttgtttt ctgattacat ctcgactgta ttgccttcat taggtgaaag
     6601 cggcgtgcct acacagacat tcaaaaatta tttggtgcag ctgctgccag agttttcttt
     6661 actagatgaa cagcaacaag aaagtggttt tttgctaggg gaaaaagatc cgatacaagt
     6721 gatgaaaagc ggattgacct tagtcgatca gattgatcgc tatatccaag gcatcacaaa
     6781 ctacggtccc ttgttccgtg atatgaaaat aaatggccgt acgatcctat caaaggaatc
     6841 gattcgccaa tggtataaag aaacaaatga acaattacct ttacatcata gactttctct
     6901 attacaaacg aagctcctga aaaaattagg cggtctgcaa aaagacgaaa cacgtcagca
     6961 atgggtcaaa gatctggcgg aagaacagct ccaagaacta tacgcaacag atccaaatct
     7021 tgaatataca gaacaaaagg agcgttcatt acgtaaaaaa ctggccaacc aaatcgtacg
     7081 taaaagattc cgtcgaatcc atcgagggat catgaattat caatttgtca atataacgaa
     7141 acaatatctc cactttctac aaactgtgcc actttctctt ctaacacgac ataaaatcag
     7201 cgagaccagg tggcaggaac atttgacaac tgttcgtaca cacttgcgtc aaagagaatt
     7261 gcgtcaagag gatgctgtac tattcttttt actgatgcgc cgaacatatc cagtgacggt
     7321 tacacacaaa gctcgctata tctttattga tgaaatgcag gatttcgcac catcacaagt
     7381 ggcattgctg caagccttgt tcccaaaagc aaatttgact ttctgtggag atctaaatca
     7441 aaatgtcttt ggaaatgaaa cgatcgttgg atcattggaa gaccttttcg tggaccgtga
     7501 agtcactcgt ttccaattaa cgacaagcta tcgctccaca aaagaaatca ctgattttgc
     7561 taatcatttt attgtgaaag aacatcaagt agaaacaaca gcaagaaaag gagaccttcc
     7621 tttgctcatc caaggaacaa cagatgatat caaattgaat tggttagcag ataccattcg
     7681 aacgactcag ggaaaagccc ggtattggcg tacagcaatc atagctaaaa cacaagaaga
     7741 atgcgaagct ctttatgctc aactgccaga cgagttgaaa caacaagtcc agctgatttt
     7801 aaatgagagt gactttatga agcgttccat tattttactt ccagcctttt tagcaaaagg
     7861 tctggagttt gaccgagttt tcttgtggga tgtagatgca gaacattttt cttctcagca
     7921 agacaaattg atcctttaca ccatgtgtac acgtgcgatg catgaattga ccctttttac
     7981 ctccaataat gaaagtcctt tgttaaacaa tatggatatt tcatcttatg agaaaaaagt
     8041 actgtaagta aaaagacaac tgctatcatt aatgttagca gttgtctttt tatttacaat
     8101 aataattgca gaaaaagtaa ttcaatcatt taaggtcatt tattctatat ataaatccaa
     8161 atctgtttca ggatatttta atttgacaag cgaaataatc attttcagtt cttccatgtt
     8221 ttctggatat tctgggtcaa ataccttgct atgacggcca atcccgccat tgtcgatcaa
     8281 tcggtaaatt cgattccatt caatgaattg gtcaattgat tggaaattgc tgattggata
     8341 gccgccacaa tccaaaatct ctgatacacg aaatgctagg tatgcactat cgatcacttt
     8401 attttttttc gtgtctatct tctcatttct ttcgataaga attttttcag gacgatgttt
     8461 caagctccac tctaatagta gaaagaaagc ctccatccga tactcttctt caaataaccg
     8521 aaaggctgaa tcagaacgac gaaaaagcag cggaatcaga taattttgtc tataaatcag
     8581 cattggtgcg cggtccacca tacactctaa ataacggttc agataccctg taccgacata
     8641 gtttcctaat cgatagcctt caacgccgaa tcttgcgtat ttttgcgcta attttgaaca
     8701 atcagttacg ttcacagcta actcttctac tttgcggtaa ccgcttaaga tacgttcaaa
     8761 aggttctaaa gtgatttttg cttccatatt cttccccgtc catcttccac atacttcata
     8821 tcatttcaca tgatacttat gctctcatca taccacaggt tgaattagaa aagaaaataa
     8881 atcacacaaa aaagcaagac aatctgttag tgagattgtc ttgcttttcg aaaaagactg
     8941 aatacttttc gttaaaactg gttgcttagt cttctaatgc cacattatgg aacacatgct
     9001 gtacatcttc tagatcttcc agtgcatcaa tcattttttc aaatgtttct tgatcttctg
     9061 gagatagtgt tacctcgttt tgagggatca tttggatctc agcgattgaa aaatcagtga
     9121 ttcctttttc ttttaatgct tcttgtatac tatggaaatc cgctacttca ccgtaaacga
     9181 tgatttgtcc ttcttcttcc attacatcgc gaacatcgat gtctttttcc attaggtatt
     9241 caaggatctc gtcagcgtct tctcctgcaa aagcaaagat tgctgtattg tcaaacatgt
     9301 aagaaaccgc tccggaaaca cccatgttcc cgccattttt cccaaatgct gcccgtacgt
     9361 ctgctgctgt tcgattcaca ttgttcgtca atgtatcaac aatcaccatt gagccgcttg
     9421 gtccaaatcc ttcatagcgc aattcagagt atgtttcatc tcctgcacct tttgcttttt
     9481 cgattgctcg gtcaataata tgttttggta cattataagt tttagcgcga tcaatgacga
     9541 aacgtagttt ttggtttgca tgcggatcag gatcacctga ttttgctgcc gcgtagattt
     9601 caatcccaaa ttttgcataa atccggctat tattagcatc tttagcagtt ttctttgcta
     9661 cgatatttgc ccatttacgt cccatatttt cacttcgctt tctgatttga tagtttcata
     9721 acgcaaaaac gtcctgttcc attatacttg tgaatcacga ttctgtgaag ctttttttct
     9781 ttcttccttc attcttctca tttttagtca attatctata ctcggattgg gatttgcccg
     9841 aaattctgtt ccggaatatt gcctcccaat tgatagattt gatcacttct aactaacagt
     9901 tccgcttctt gtgcctcttt ttttccgatt cgggaataaa tagaaatctc ttttttttct
     9961 tttaagaatc ttcttccagc atcggtaaat ccaagtagtc gaagataatt tttattttgt
    10021 tggacaggaa tctcttgctt tttgatattc agcaagacat aacaagataa tcgttggatc
    10081 cgtgtccatg tatatctttt tgtttttacc tttgacacaa aatcctcaaa actatcagca
    10141 gttgcagctg ctgttttcat gcgcgattct attccttcgg tcatctgata gatatctgtc
    10201 agttccagca aagtagaaga taacaaccga taacgcaaat acggccaata agattcccaa
    10261 gtctgcagcg aacaagttga aagatcttct tctgtctgag gcggcacaag tccagctatt
    10321 ctttttcctt cttctaacgc atggcgaatg gctgttgcac tggctatcct ctgatctgtg
    10381 atgatttttt catgatagcc tgcactttta cgtcggatcg gatgtaattc catagggtgg
    10441 acatacttgg cattttcctt tgcataactt aatcccaaga tatgatttgg cgaagaaaaa
    10501 tcaagcgtaa tatcgggata cagactgcga aaaacctcag tcattttttg cggataacta
    10561 tacgtttgat cagacagttc ttgaaatgcc tcttctatcc tatcttgatt ctcttggaca
    10621 aagcgtccga aactgctgta atcaaacgaa gtctcttctt ctgttccaaa acacaaactg
    10681 tcacagtcca gtgtttgcaa gatttttact gcacctttag caaaatagtc agctgactga
    10741 accgcccatt cgacgggaag ctccactact aaatccacac cattttgtaa agcttcattc
    10801 gcgcgatgcc atttatcgat gatagccggt tcgcctcttt gcaaaaaatt cccgctcatt
    10861 acagcaatga cgacctctgc tcctgttatt tttcgtgcca tttccacatg atagcgatgt
    10921 ccattgtgaa aaggattgta ctctacgata atcccgcagc ttttcatgcg catcctcctt
    10981 catagttatc gataaaaaag aaatagagac aagtaaaaaa aggcaaaaat cggaaaaaat
    11041 acccgaacta tataaactat tctcgcaaac acaatacttc tgcgacgaaa agctgcactg
    11101 caagagcaat gcctatatcg ttcggatata taatttatgt tgaagattta ttcttttctt
    11161 gttaactcga gcaggcaaaa ccgctagtct ttgtatgcta caaagaacca gcgctttgat
    11221 ttctctgatg ctgcttcgtc tgtaaaatca gaaaatgctt cgactttcga aaagccgctg
    11281 ttttccagca ttcttaaata actttccaat ggataggtac gctcttgatg tagttcatct
    11341 tcacgaataa atcgaccaga cccttcttgt tcttcaacga aaaaggtaag aaaatgttcg
    11401 atgctgtgtt tctcgcttcc tggataacta tcccacaaaa aagcaaaatt atccccttga
    11461 taatgatagc tatattctgg aaaaacctca tctacttgat aggtggagtg tacgtcaaat
    11521 aaaaatcggc cattttcttc taacgcttga tagacttcat caaacacttg ctgtacctct
    11581 tgtctatttt tcatatagca cagcgaatca gaaaaacagg taattgcctg atattgtccg
    11641 atttctgata aatcgagcat attgccttcc acaaattgga tcgatgcgtc ttcttcatat
    11701 gctcgctggc tggcaatcat caacatctct tctgaaaggt ctaaccctgt cactttatat
    11761 ccatcttttg caaaggcaac cgctaatgct ccagtaccgc atgccagttc caaaatattt
    11821 tttgtgtctt cttgtaaatg acgtttcgaa aaagctaacc attgctgata aagtgtttcg
    11881 tccatcactt catcgtaaat gaaagcaaat gtttcataag ccatatgtta atccaaccat
    11941 tcactaagat cgactagcgg agcatctgac catagttttt ccagattata gaaatttcgt
    12001 tctggcgcat ggaaaacatg tacgatcaca tctccaagat cgatcaatac ccattttccg
    12061 ccttcttttc cttcaatacg ttttacttca tatttgtttt cttcttcttt atcaatgatt
    12121 tcttctgtaa ttgcgttgat ttgacgttcg ctatttgctg aacagatcat gaaataatct
    12181 gccaacaagg aaacatttcg aacgtctaaa gcgataatgt cttctgcccg ttttgaatct
    12241 gctgcttcaa tcgcaatttt taattgttct ttactatcta tgatgatttc ctcctaatat
    12301 gagcagctta tttttttgct acccagtaat tatatgtttc gattgttttt ggataaatct
    12361 gttgttcttg cgcaagcaaa tgggccaatg tatgttttgt ttcaaatgct acagcttcgt
    12421 ctagatcagc ccatgcaatg gctcgtgcat cttgtacccc aggaaaatct cgacctggtt
    12481 caatatagtc tgctacataa atgattttgt caaggagact catcttagca gccccagtcg
    12541 tatgcacccg gattgcatga agaatctctg catcggtgat tcctaattcc cgctctacaa
    12601 acgaagcgcc aacaagacca tgccagattg cattattata attcaataac tccggatcaa
    12661 aaccgtctct tacgattacc attttgaact cctcatctgg tcgttctttt gcataatcat
    12721 gtgtcaatgc agcaatacta gctttttcag gagaagcacc gtattttttg gcaagagcca
    12781 cggctgtttc ttctactcca agcacatgct tgaagcgttg ttcgctcatt ctcatctgta
    12841 ttttttgcat caggttttct ctagaataag cagtatattc attactatag tccatattca
    12901 tacaaccctt cattttgaat ataatcaatt actttatctg gaacaaggta gcggatagaa
    12961 cagccttctt tgatcttttg acgtattttc gtggaactga tgtcgatctc aggtacatct
    13021 acccaaatta caggatatgg agtatccgtt gtgtatcctg gtcgacgtat acccacaaag
    13081 ttgaccatag aagtcagctc atcgatttta taccattttg gcaaatattc taccatatcc
    13141 ccaccgataa taaagtaata atctgtatcc ggattgtttt gcgtcaattc cttcatagta
    13201 tcataggtgt aacttttccc accacgagct aattcaatcg tttcaatctg caaaaaagga
    13261 ttgtcttcca ccgctaattc caacatgttg agtcgatgtt ttgcatcaat cgtctgtttc
    13321 tcatcaacat gaggtggctg ataagaaggc atcaaaaata cgcggtcaag acctaaattc
    13381 cggccagctt gttcagccat cactaaatgt gcaaggtgaa cgggattgaa tgttccgccc
    13441 aagatcccta cttgcttttt cttctctaag aattgaggca tttcttcagg aaataattgt
    13501 gaacgaacaa atgtcttagc ttgcgtattc atcatggtca ctccttatat tgcttttact
    13561 tcttttgata gcctttgata cttttctttt gtggaaggtt tgtataatac aataacacgt
    13621 ccaatgattt ggacgatttc gcaatctatt tctttttcta atacttgagc tgcttcttct
    13681 gcaatctcat ctgtattttg aagtaacgat actttgatca gttctctttt ttctaaagct
    13741 tcttcaattt gaacgatcat tgctgaattg atcccacctt ttccgatttg aaaaatcggc
    13801 tgaagatgat gcgctttgct acgtaaaaaa cgcttttgtt ttcctcttaa actcataatt
    13861 ttccttcttc cttaaatcaa tgctttgcgt ttcaatacat ctacaccctt tggcgcccag
    13921 cccgcaatta ctactggcgc tgttacagtg atccagccta gaccggcaaa aacgatgtct
    13981 gttttttctt tcacagaaaa ctcaaaacga acaagctccg gaaatgcctc gacttcatca
    14041 ggacgaggag gctgtaataa tccacctaca tgtttttgat aaaattcgtc tgctttttcc
    14101 agtttcgttc gatgaatatt tacatcattt gacacataag caataaatga agcacgattg
    14161 ccgctgataa aatcaaatct tgcaagtccg cccaagaaaa gtgtttgccc ttcattcagc
    14221 tgataaactt taggtttgat ttcttttaac ggtgcaatga tccgcaaatc ttttttacct
    14281 aagtagtgcg ccatttgatg gcgatggata attcccggtg tatcaatcaa aaaatgcccg
    14341 tcatctaaag gaatctcgat tttatccaag gtcgttcctg gaaactgcga agttgtgatt
    14401 acatcttgta ctcccgctgt atttttgatg atttgattga taagcgtcga ttttccgaca
    14461 tttgttactc cgacgatata gacatcccgt ccatcgcggt attcttcgat tttttctaat
    14521 agttccgtca tctcatgaga tttcttagca ctagttaata atacatctac tggacgaatt
    14581 cctgcttcgt aagcacgttc cttcatccat tgtgttaatt tagttttttt caatgattta
    14641 ggtaatatat ccactttgtt cccaactaaa agtactggat tgtcaccgac aaaacggtgt
    14701 agtcctggga ttagtgaacc attgaagtca aaaatatcca ccacattgac gatcaatgca
    14761 tctttttgcc ctaattcatt cagtaagcgc aaaaagtcgt catccgtcaa tgagacatct
    14821 tgggtctcat tataatggcg taatctgaaa caacgctgac aatagacttc cccggaagct
    14881 aaaccttttt ccaaagccgt ttgcggcgtg tatcctaatt caccgggttt gtttgtttgt
    14941 ataacggcac cacagccgat acattgtaat tcttctgtca ttcgattcca cctcgccagc
    15001 ccatatcagg gtgttttgct aatagatagg ccataattct tttttcccaa aaacgattaa
    15061 attgtgtctt ccaactatct gttgcgataa tgggttttac aaggatgcta cgtatccctg
    15121 cacgattggc tccacgtata tcagtcataa tctgatcgcc taccattact atctcatcat
    15181 ctgctaaatc caattgtttc tttgctatgt tgatccctct tgctaaaggt ttcattgctc
    15241 gtgcaacata caaaagatca aatttttcaa tcgctcgcgc gacacgtttt gaattattgt
    15301 ttgaaacaac aacaacaggg attcctgcat ttttcatttc taaaatccaa gtaagcaact
    15361 cttctgttcc atctggatta ttccatgcaa tcaacgtatt gtctaaatcg gtcaatactg
    15421 ctttgatgcc tagattcttt aattgggcag gtgtaatctg atagatggct tctaccatcc
    15481 atgttggttt gtaatttgaa aacatgctgt ctcctcatct gcaaaaaatg aaggagcaca
    15541 taaagtactc ctcaaccccg tattatactg catatcagca aaaaaaacta cctctatact
    15601 cgttcgtctt tcagaaaaag catttcttta caaaatgacc aaaaataaag attgatcaca
    15661 gaaaaattta gaaacaaaaa acaactaata ttggcggaac ctcttgtatc gtttttccag
    15721 taatttatgc tgatctaatc gaacaagttc cgtcaatctt tcaatcaacg cttttttcat
    15781 aatgtgtgcg actttatatc gtggcaactg ctcgccgttg aacgtttcag gaataactcg
    15841 atcaacgatt cctaactcgc gtagttctgg cgcagtaatt ttcatcaact cagctgcttc
    15901 tgcggcgcga ctaccgtctt tccacaaaat cgacgcaaat ccttctggag aaagcacagc
    15961 ataaatcgtt tcttccagca tccagacttc atccgctaaa cctagagcaa gggcaccgcc
    16021 acttccacct tcaccaatga tgatggcgat gatcggtacc tttaaatcag acatttcgag
    16081 cagatttctg gcaatagcct ctccttcgcc gcgctcttct gctcctacac cgcaatatgc
    16141 acctgctgta ttgatcaagg taatcaccgg tctattgaac ttttccgctt gtttcatcag
    16201 tcgtaaagct tttcgatagc cttcaggatg gggagaacca aaatttctgc ggatattttc
    16261 ctgcagatcc cgtccttttt gaataccgat cactgttacc ggtttgccat ctaatcgagc
    16321 gattccacca acaatcgctt cgtcatctgc aaataaacga tcaccatggc attcctcgaa
    16381 agaatcgaag atctcttcaa aaaaatccaa gcttgtccat cgttctttag ctcttgctaa
    16441 ctgaacgaca tcatgtgctg tcttatccac gatctatcca tcctttctgt gtatgcaatt
    16501 caagaagcgt atggattttc tgtttcaatt ccatgcgggg aacaatctga tcgacaaaac
    16561 catgagataa taagaattca gccttttgga aatcttctgg aagttcctgc ttgatcgtct
    16621 gttcgatgac gcgtcttcca gcaaaaccga tcaagctttg gggttctgct agaataatat
    16681 cgccttccat tgcaaaactg gcagttactc ctccggtcgt cgggtccgtt aaaacagtta
    16741 gataaaaaag accttcattg ctatgccgtt tgacagcagc agagattttt gccatctgca
    16801 ttagagaaaa gattccttct tgcatccgtg cgccacctga agctgtgaac aagacgactg
    16861 gaagactttc ctttgttgct cgttcgaata atctggtgat cttttctccg acaatcgttc
    16921 ccatacttcc catgataaaa ttaggatcca tcactccaat aatgaaggga atcccctgaa
    16981 tggttgcttt accagtgagg actgcttcat gaagtcctgt tttatcttgc atttttttaa
    17041 ttttttccgt atattcggga aatgctagcg gatctttggt tacaagatcc gtatcccatt
    17101 cttcaaaact tttttcatca ataatcaaag ctagacgctg ccatgcaccg attcgaaaag
    17161 cgtatccgca atgagggcat actttttctt caccgatgtc tttcgtataa aggatatgtt
    17221 tgcaatttgg acactttgcc cacatgttat caggaacaga tggcgagtta gcaggtttgc
    17281 tctctctatt aggattgatc cgaatatatt tctttttctt aaacagagcc atctatgcgc
    17341 ctcctatctc cgtttctggt gtccaattcg gtaaaaattc ttcttgtaaa aatgctgtgc
    17401 tgtaatcacc tgctatgact gcaggatgac taatcaaatc catctgaaat tctgcgttcg
    17461 tgatgattcc ttctgtgata aattcactta aggctcgctg catcttcatt aatgcttcaa
    17521 aacgcgtatt tccatggacg attatttttg caatcattga atcataatat ggcgggatcg
    17581 tatatcccgg gtaaactgca ctgtctactc gcagtcccat cccacctgct ggaagcagta
    17641 agttttggat cttccctgga gaaggagcaa aatggaaagc aggattttct gcattaatcc
    17701 ggcattcgat tgcgtgtcct tggaatacta cttcttcttg acacacattc aaaggctcac
    17761 cggctgctat ctccacctgt ttttttacta aatcgattcc tgtgaccatt tctgttactg
    17821 gatgttccac ttggattcga gtattcattt ccatgaaata aaaatcgcct tcttcatcca
    17881 tcagaaactc gatcgttccg gcattttcat aattgactgc tttagctgct cgaacagcag
    17941 cgtctcctag tgcttctctt tttgtttgtg agatcactac agaaggagat tcttccagta
    18001 ctttttgatt gttgcgttgt aaagaacaat ctcgctcgcc taaatgaatg acatgaccaa
    18061 atcgatcacc taagatctga acttctatat ggcgagcagg ataaatgatt ttttccaaat
    18121 acatatcatc atttccaaat gccgctgctg cttcttgctg agctgaagaa aaatgctgtg
    18181 ggagctcttc ttttgattga actttccgaa tgcctttccc gccaccacct gcagctgctt
    18241 tcagcatcac tggataacca attctgtctg ctatttccaa tgcttcttct actgtagata
    18301 atacaccagt acttcctgga atgactggga catttgcttc ttgcatcaat tgacgcgcat
    18361 tgatcttatt tcccattgca tcgatcgttg cacttttagg accaataaat gtgatattgc
    18421 actcttcaca catggaggca aatcggctat tttcagataa aaaaccaaat cctggatgaa
    18481 ttgcttcggc ttttgtcacg attgcagcgc tcagtacttc ctgaacattc agatatgaat
    18541 caacggcttt tgccgggcca atgcagattg cttcatctgc taactgtgta tgaagtgctt
    18601 cggcatctgc ttctgaatat atcgctacgg tttggatccc tagttcgcgg catgcacgaa
    18661 tgatacgtac agcgatttca ccgcggttag ctatcaatac tttagaaaac atatttcacc
    18721 tatccaatca taaaggttaa ttccgcttcg gctacttttt taccgtctac tttagcgatt
    18781 cctttgccta ttccggctga agctttcact ttcaatatct caacttcaag gtataaggta
    18841 tctcccggtg taactttctt acggaatttt gctttgtcca atccgccgaa ataagccgtc
    18901 tttcccttga attgttcaag agaaagtaat gcaactgctc ctgcttgagc cattgcttca
    18961 acaatcaata cccctggcat aacaggttct tctggaaaat gtccttgaaa aaagggctca
    19021 ttgatcgtta catttttctt agcaacgatc cgttctcctt caaccatttc ttccactcga
    19081 tcaatcaaaa gcattggata acgatgtgga atgatttctt ttatttcttg tatgttcatt
    19141 gttttccctc tttcctgctt tgcttatttt tgctgaatcc taaagagtgg ctgtccatat
    19201 tcgacaactg actcgttttc tacaaggatt tctgtgatca ctccgtcggc ctcactagtt
    19261 atctcattca tcagtttcat cgcttcaata atgcagataa cttctccttt ggcaacttga
    19321 tcacctactt ttttgaaagc tggtttgtcg ggcgctggtt gcagataaac gattcccacg
    19381 attggcgaaa caacctcgat tccatcaaat actgccgatt ctttttcttt cattatttct
    19441 ggagcagttg ttgcttgttc aggtatgaca ttgactggct ccgaaactgg caacgtttcg
    19501 tttgtttgaa ccatattcat gcctcgactt gttttatttt tattcatata caattcaaat
    19561 tgaccttcac gcaaatcgaa ttccgttagc gaggattgat caaattgact gactaattct
    19621 ctgatttcat tgatatccaa aattattcct cccagcgttt catgcacaga accgcattat
    19681 gtccgccaaa ccctaatgaa ttactcattg cataagtaaa acttgcaggt ttgctttcgt
    19741 ttttgacaac tgtcaagtca atctcaggat cttgtttttc tacattgatc gtaggtggga
    19801 taaattgatg ttctaatgcc agcaaagttg ccaaggcttc gatcccgccg gctgctccca
    19861 ataaatgtcc agtcatcgat ttcgtgctgc tgacgtaagt atctctgaaa ttttcaccta
    19921 atgcgatttg gattgctttt gcttctgcct ggtcattggc tggtgtactt gttccgtgag
    19981 cattgatata tccgacttgt tccggctgta caccagcttc ttccatcgca agcttcattg
    20041 ctttagatgc accagaacca tctggagtag gagctgtcat atggtaagcg tcacagtttg
    20101 caccgtatcc aacgatttct ccatagattt tggcaccgcg agcttgtgca tgttctaaag
    20161 attcaagaac tagaacgcct gctccttcac ccatcacaaa accattccga ttttcgtcaa
    20221 acggaatcga accacgatta ggatcagttg attctgagag tgctgttaaa gctgcaaatc
    20281 cagcaatccc catctcacaa atcgttgctt ctgttcctcc agcaagaatg acatcagaat
    20341 agccatgttt gatgttacgg aatgcttccc caattgaatt cgttccgcta gcacaagctg
    20401 ttacgatagc tgtacaaata ccttttgcac ctacacgcaa agcaatattt ccagctgcca
    20461 tgttggcaat cgccattggt acgaacattg gagaaactcg ttttgcacct ttttcatgta
    20521 gacgaataac ttggttttcg attgttggaa gaccaccgat cccagatccg atgatgacgc
    20581 caaaccggtc ttgattcatt ttttctttat ctaaaccgct catctccatt gcttctaaag
    20641 cagcgtagat tccatagatc gaaaacatat ccattcgttt gctatctttt ttaataaaat
    20701 atttatcgta agggaaatct ttgacttcac ccgctaaggt aatccctgtt tctgaagcat
    20761 caaatttagt gatcggtgcg atgccattcg tcccctcttt taagctattc aaaaagctgt
    20821 ctgcgtcatt tccaataggt gaagtgactc catagccagt aattaccact cgattcattt
    20881 gtttgctctc cttttcttat ccgtgcatga ccaatccgcc atcgacattg atgacttgtc
    20941 cggtgatata tggattttgt gctaagaaaa ctgctgtttg tgcaatatct tctacttgtc
    21001 cgaaacgctg catcggaatt tgcttttccg cttgttcctt gactttgtct gctaaaactt
    21061 ccgtcatatc agttgtgatg aatcctggcg caatggcatt acatgtaatg cctctagttg
    21121 cagcttctcg tgccacagat tttgttaatc ctactacgcc agctttacta gctgcataat
    21181 ttgcttgacc aatatttcca atcaacccag atacactaga gagattaatg atggcccctt
    21241 ctcgctgctt catcattttt ttcaatacgt gctgcgtcat attgaaggtt ccaatcaaat
    21301 tgatatccag acattttttg aaatcttctg catccattcg catcacaagt ttgtcatttg
    21361 tgattccggc attgtttact aacacatgga tcgatccaag cttttcttct gcttctttga
    21421 tcatttgtcc cgctttctca taatcagaga tgtcaccgga aactcctacg cattttacac
    21481 cgaatgcttc gatttcttct atcttttctt ttggaatctc tccgcgacca ttcaaaatga
    21541 tatttgctcc agcttttgca aaagctaagg ccatcgcttt gccgattcct cttgtactac
    21601 ctgtgataaa tacatttttt ccttttacat ccataacgtt cctccttata gtattgctat
    21661 tgtttcatca aatgtctgct tattctcaac acgcagaact ggtattgttt tatcgatttt
    21721 tttcataaat ccgcttaata cttttccggg gccgacttcg atgacttgtt cgatgccgat
    21781 tgtttttaac ttatggatac tctcatagaa acgaacaggt tgcatgactt gctgttcaag
    21841 tagtcccgcg attgtttctt tcttcattat ttccgttgtt gtgttgctta tgataggaaa
    21901 agaaggttca gagaattgaa tctgacttag gtccttcgcc agtttttttg ctgccggttc
    21961 taaaatagct gtatgaaaag gcccgctgac atttagaggg atcattcttt tgaacccttt
    22021 ttcttttaat agtaaaacag cttcgtctac tgctttttct tcgccaccaa tcacgatctg
    22081 ctgaggtgta ttataatttg caggagatac gatgccgtac ttgctagctt catgacagct
    22141 ttcctcgatc gtttcaatcg gcgcattcat cactgcaacc attttcccgc ttccagctgg
    22201 ggcagcttct gtcatataag cgcctctttt tgctactaac gcaactgctt cagtgaagga
    22261 tagcgcatta ctagctacta atgccgaata ttctcctaaa ctcagtccta aagctgcatc
    22321 tgggatgatt ccatgctcct tcaataagcg ataatacgcg atgctgactg ttagaatagc
    22381 tggctgtgta tattgcgtct gatccaaacg ttcattttct gtaaagcaaa gttcagccat
    22441 atcgtagccg agaatctcac ttgcttcatc aaatgtctgc tttacaacgg cttcttcata
    22501 taaatctttt cccatacctt gatattgtgc accttggcca ctaaataaga atgctgtttt
    22561 catgtttttc tccaatatat ccagaatata tgtttgattc ttcctattca aaaaggccga
    22621 gacattccta tttcaggaaa tgtcccagcc cccaacctaa ttttaatgat tatgcagttt
    22681 gtttttctac gtaagtcact aaatcttgaa ccgttttgat tccgtcttct gtttcgattt
    22741 tcacgtcaaa ttcatcttca atttcattga tgatttggaa taaatccaag ctgtcagctt
    22801 ctaagtcttc ttggatgtca gtagtcaatt tgatttcttc agtttctttt cctaattctt
    22861 ctgcgatgat ttcttgaatt ttttcgaata ccataataat atattcctcc gatattgttt
    22921 gctttttatt tttataattt aacagtctac aactccatga gaatacttcc ccatgttaaa
    22981 cctccgccat agcctgtaaa tattactttc tgctgttttc ctaaggtgat ctttccacta
    23041 gaaactgctt catccaataa gatagggatg cttgcagcag atgtattccc atatttatcc
    23101 atattggtca ggaacttttc ttgcggcatc tttacttttc gggcaatttt ctcaataatt
    23161 ctgaggtttg cctgatgcaa aagcagataa tcaactggtt tgtctttcat gacttcttgg
    23221 atattgtttg caacgtctcg tacggcaaaa tcaaaaatat ctctgccagt catttgcaaa
    23281 taatacgaac cttctgaatc ttctgaatag aacggatttt tattttgctg gtagccagcg
    23341 gtcaatgagc tacttctggt accgtcagct gctaattttt ctttcagcaa ttttttctgt
    23401 ggattcgctt ctaagagaat cccacctgct ccatcgccaa acaacacagc agtcgtacga
    23461 tcagaccaat cgattagctt agatagtgtt tcaccaccaa tcaccagtcc cttagttgag
    23521 ccgcagcgga tcattttttc agccatgctt aacgcataaa caaatcctga gcacgcagca
    23581 ctgatatcaa aagcgatagc atgagttgct cccaatttcc cttgtacttg tgctgctaca
    23641 gatggtgtcg tgtaatcggg cgacattgtt gctactaaaa taaaatccaa atctttaggg
    23701 tttagatttt tctgttttat caatttttcc gccactaaaa tgcataaatc agaagtctcc
    23761 tgatttgtta cacatcttcg ttcacgaata cccgtccttg acgaaatcca ttcatcagag
    23821 gtatccatca tcttggaaag atcattatta gtaataatct tttcaggaag gacgctggct
    23881 gttgcagtaa tcattccaaa ttgttccatt aaatatcctc acttatagtc ttgcaagaag
    23941 tcgtgcaagt ttttcagtgc atccagcaag gcatgctgtt cctccgtagc cattccgtct
    24001 aagatgttct tgaccatttc acgatgaaaa tgctgatgga cccgaaatag taactttcct
    24061 tttttcgtta gtcccaactt tacaacgcgg cggtcatctt cactgcggat acgttcgaca
    24121 tagccttttt ttactaactt gttaatagca gtggtcaagg tcccaacagt aattgatagt
    24181 tctttggcta cttctgaagt tgtttttttc ttgtacatcc cgattgcctc gatcgtgtgc
    24241 atctctgtga ttgacagatc tttgaattga gattttttca gctcagactc ttcgattgtc
    24301 aaaatatcat tgaagacact aacaagataa tcatttactg tttttaaatc tggttccatg
    24361 ttccaccgtc cactaattag tttgattatc aaatcttttg atgttcaaac tatatcttaa
    24421 cttttttcaa aatgcaacct taaaagatca tttttttact atatgagctt tttttaagat
    24481 tttactcatt taaaacaact gataaatcag catttataat ttcttttatt agaaaaatca
    24541 tctatctcac atattttgat tatcaaagta ttttctgtga acagaactat agaattccct
    24601 aaaacttgtc cattatttca aaatttgtta tagtcttttt attcaaagcg taaaaggaga
    24661 tcctatgtta aaagaaaaaa tgaaaactct ccccatgctg ccgggtgtct atcttatgaa
    24721 agaccgagat ggtaaggtca tctatgttgg taaagcaaaa cggctcaaaa atagagtaag
    24781 cagctatttt catcagaata aacagcattc aaaaaaagta ttacgtatga ttcatcatat
    24841 aacagatttt gatttcgtcg tagtagacac ggaattagat gctttattgc tcgagtgtca
    24901 attgatccaa cattaccgtc ctttttataa ccgacaaatg aattatttca gtaattataa
    24961 ttatgtacat attaccaata agggatttgt attaacagat actcctacgg ctcgaacata
    25021 cgggcctttt cgtctctata aaaaaatgcc gtctattttg cgtataatgg aagaaactta
    25081 tcaaatgcct tggttgtctg aaattagttt gttagctttg cgagtccagc ttcctgacct
    25141 acaggaaatg acttttgaac agaaaaaaaa ggaactccaa gggctgtttc aaggccgaaa
    25201 taaaaagcta ctgacttact tgaaaaaaag acaacaacat ttcatctatc agttgaattt
    25261 cgaaaaagcc ggtatgcttc aaaaagatat cgaattagtt acttatttta ttagacggat
    25321 ccaggaacaa aagcagtttt taagaacacc atcgctcacc ttcagcatgc cattagcagc
    25381 agatgagagt cagaaaaagc actacctcgt ttgttatggt caacttgcag aaacaatgat
    25441 tacctctggt gacgtttctc ctgacttcta ttatgaaaag aaagaagctc atttatcttt
    25501 gaaacgtcaa ttgtcaaaag aagaaatcga tcctgtacaa atcttgatca gttaccgcaa
    25561 aaaattagaa aaagaacagt ttgaaattga actgttaaac aaaaaagaag ctgaaaagca
    25621 actaaattag ttgcttctcg gcttctttta ggcatcttca acttcaaatg tcaattatcc
    25681 taaaatttat tggattaagt catagatttc catcgctacg atatcgatat tatcaaattc
    25741 gtattgctga tgagtatctg tttcagttaa ttcaaatgta tcggtcgctt tatcataagt
    25801 tactgtacac ttctctacgc cttctttttc aaaacggcga acttgcagtt cattatctgc
    25861 tgactcgacc atagcctcca gtcgttttat gattgcgact aattgtgaat gtgtcatcgg
    25921 aggtcctcct tttcttattt ctgttatatt gtatcaaaag catagcctac ttgcattgat
    25981 tttctctaaa aaaactcgtt ttttatgatt ttttttctgg tatttttcct tcaggccacc
    26041 aaagtttgat caaagcttgg gttccgtcat tctcaaaacc tttatctgcc aactcttcat
    26101 atagtttcaa tgcttgggta gttgctggca atacaagatc cattttttct gcttcttcta
    26161 atgcaatctt taaatctttg ataaaatgtt tgacaaaaaa accaggtgtg taatcttctt
    26221 ttaaaatacg aggagaataa ttgcttaaag accagttagc tgcacttcca cctgaaagcg
    26281 tctcaatcac tttctttaaa tcaagtccgt ttttttgtgc atagacaagc atttccgtca
    26341 ttcctgtcat cgtacctgca atcatgatct gattagccat ttttgtatgc tgacctttcc
    26401 cagcagaacc atgcaatgta aatgtcgtac caaattcttt gaataaaggc agtacttgat
    26461 cataaacggc ttcttctcca ccgaccatga tcgtcagtgt accattcttt gctcctaagt
    26521 caccaccgga taccggtgca tctaacgcat ctgcgccttt ttcttttgct gtttttgcaa
    26581 ttttttctgc caggcttggt gtacttgtag tcaaatcaac taaaatatgt cctgagatat
    26641 ctgcagaaaa aatccccgac tgtccatagt agatttcttc tacatctttg gggtaaccta
    26701 ccattgtaaa gagaatatta ctttgttctg ctaccgcttt tggtgtatct gcccagatag
    26761 ctccttctgt tactaaagga tcagcttttg cttttgtccg attatagaca gctacttcgt
    26821 gtcctgcttc tagtagatgg cgtgcgacag cacttcccat gacgcctgtt ccgataaaac
    26881 caagtttcat tttttcgacc tcctaaaaat caaaaatcct tgcttctatg ctagtatacg
    26941 ctagaaacaa ggattttttc ataaaaaatg tttgaatttt atcttttaaa tgaatttaat
    27001 tttattttga ttcgttcaat cgagtcttct tcttcttttc ttcgcatctc ttcttcatga
    27061 taaacaacca tgtcttgata aaattcgatc atagcttcgc cgaaatgtgt agatgaaata
    27121 tcataaagtt tagcttctaa aacttttgga tctttaggaa cttcactgga aatataatca
    27181 ataagtgctg gtgcaaagtc ttcgtcggtt tcaaaagttt tccctaatga aggatgatcg
    27241 aacaatcggt tcaaataagc atttccttcc acgacacaag gtgttccggc tgccatcgct
    27301 tctgtataag taagcccttg tgtttctgaa gtagaagcac tgacaaaata atctgccgct
    27361 ttataataaa gtgctacatc attgttgtcc acttctcctg tgaactgcac aaattcttct
    27421 aactgcagtt ctgagatcaa ttcctctaac tcttctacat aaggtccttt tcctacgata
    27481 actaatctgg cgttaggata agcggagatg acctgaggaa acccattaat caaggcttgg
    27541 atatttttct cataagaaat cctgcttaaa gaaagaatca tcaaatggtt ttcctgaaga
    27601 cctagtgact cccttaattt gctgatatcc tgctgagtga tatcagaacg tttgaatttt
    27661 tcaatgtcga taccagtagg aatcacgcga agcggtgcta ttacaccata gctgcgtaat
    27721 gtatcaataa cacgttcact tggacaaact acacctgtcg tatgatttgc aaataatcta
    27781 gacaagtatt ttacatggga ttgcctgatc acttttcctt taccgatata atgaaggtag
    27841 tcttcataca tcgtatggta agtatggatc agaggtattt tcaacttctt agctaccatc
    27901 ttcccaagta tccctgctcc gaattctgta tgggtatgaa taagatctag ttctagttct
    27961 ttggcaacca aataagcata ccacatgcct ctaacaacaa ctcttcgatc cttgaaagaa
    28021 ataaatggta cacttggcat tcgaatcaca tctttttcca acttatccgc attaggatcg
    28081 gtcgttgtaa aaatatagac ttcatgccca tgtctttcta attcttgttt taatgttttg
    28141 atcgatgtag caactccgct gacttgcgga aagtaggtat cggtaaaaaa gccgattttc
    28201 atgtttttcg cctccaaact agtccttgac ctgatttact ttttttaatt tgctagagac
    28261 tttttcattc agtacacttt cgtatacatt tttaagttct tcaccgattt tattgatact
    28321 tcgctctttt gctgtttgat aaccggcttg tgtcgtactc ggtagttctt gattggtaat
    28381 ctgttggatc agtcggataa attcttcgtt tgtcttcccc atatagcagt tcttgctatc
    28441 ttctaaccag ccattataca ctggaatatc tcgaacaagt acgttttgtt tgcttgctaa
    28501 agcttctaaa acaacgatcc cttctgtctc ttcataagaa gggaaaaaga atagatctgc
    28561 acctgaatac gcaccttcta tgatctctcc acgaatgtaa ccaggaaaca ggacattttc
    28621 aggatgatct tcttttacaa ttttccggat gttccgagga atcgagtaca tgggaacatg
    28681 accaaaccaa ataaatgtgt attcgggcat cttcttagca atttctacga aatcaatgat
    28741 tcctttacgt tcgaaaaaca aaccgacaca aatgattact ttttgatccg gagacagttt
    28801 gaaatattct ctgaactttt gttctttcat tggatcaggg tgatatttct ctaagtcgat
    28861 cccattggaa atagcttgaa tcggcacacg gataccatag ctttccaaca actgttttga
    28921 ataaggtgtt ggcgtgatca gatagtccgc tttttggtac aatcctacca gatatttttt
    28981 tacgatagga gaaagctggt tcgatccgat aaaggaattt cggaaatctt cctctgtaga
    29041 atgcgcatga tagacaactt tttttccttc tcttctggct ttattgacca tattatgact
    29101 attgattccg tatgtattga tgtgcaggat gtcataatct tctttttcat ctagtgtata
    29161 agagataccg acttccgtca gagcacgttt ttgatgatca agcgcacgcc cgattccaga
    29221 ttttgcaagc atcttttcac tttcaaaata taataatacc ttcaccttac gtcccccttc
    29281 acttatatag tataacatag cctacaaact gtcactcaaa cttaagccct attttt
//
LOCUS       DAZKBH010000023        28886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000023 DAZKBH010000000
VERSION     DAZKBH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.023"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACG8OO_001011"
     tRNA            12..84
                     /locus_tag="ACG8OO_001011"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACG8OO_001012"
     tRNA            88..160
                     /locus_tag="ACG8OO_001012"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACG8OO_001013"
     tRNA            168..249
                     /locus_tag="ACG8OO_001013"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACG8OO_001014"
     tRNA            267..339
                     /locus_tag="ACG8OO_001014"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACG8OO_001015"
     tRNA            351..422
                     /locus_tag="ACG8OO_001015"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACG8OO_001016"
     tRNA            439..524
                     /locus_tag="ACG8OO_001016"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACG8OO_001017"
     tRNA            535..608
                     /locus_tag="ACG8OO_001017"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACG8OO_001018"
     tRNA            618..691
                     /locus_tag="ACG8OO_001018"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACG8OO_001019"
     tRNA            711..783
                     /locus_tag="ACG8OO_001019"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACG8OO_001020"
     tRNA            811..884
                     /locus_tag="ACG8OO_001020"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACG8OO_001021"
     tRNA            898..971
                     /locus_tag="ACG8OO_001021"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACG8OO_001022"
     tRNA            995..1084
                     /locus_tag="ACG8OO_001022"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACG8OO_001023"
     tRNA            1097..1170
                     /locus_tag="ACG8OO_001023"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACG8OO_001024"
     tRNA            1189..1264
                     /locus_tag="ACG8OO_001024"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACG8OO_001025"
     tRNA            1266..1336
                     /locus_tag="ACG8OO_001025"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACG8OO_001026"
     tRNA            1350..1423
                     /locus_tag="ACG8OO_001026"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACG8OO_001027"
     tRNA            1448..1535
                     /locus_tag="ACG8OO_001027"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACG8OO_001028"
     tRNA            1557..1628
                     /locus_tag="ACG8OO_001028"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACG8OO_001029"
     tRNA            1651..1740
                     /locus_tag="ACG8OO_001029"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACG8OO_001030"
     tRNA            1753..1826
                     /locus_tag="ACG8OO_001030"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACG8OO_001031"
     tRNA            1845..1917
                     /locus_tag="ACG8OO_001031"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACG8OO_001032"
     tRNA            1921..1993
                     /locus_tag="ACG8OO_001032"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACG8OO_001033"
     tRNA            1998..2068
                     /locus_tag="ACG8OO_001033"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACG8OO_001034"
     tRNA            2081..2154
                     /locus_tag="ACG8OO_001034"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACG8OO_001035"
     tRNA            2166..2237
                     /locus_tag="ACG8OO_001035"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACG8OO_001036"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACG8OO_001036"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9044934.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACG8OO_001037"
     CDS             3826..5298
                     /locus_tag="ACG8OO_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9044935.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACG8OO_001038"
     CDS             5381..6169
                     /locus_tag="ACG8OO_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9044936.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACG8OO_001039"
     CDS             complement(6236..6904)
                     /locus_tag="ACG8OO_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFP9044937.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACG8OO_001040"
     CDS             7019..8731
                     /locus_tag="ACG8OO_001040"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044938.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="ACG8OO_001041"
     CDS             8734..10503
                     /locus_tag="ACG8OO_001041"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044939.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11275)
                     /locus_tag="ACG8OO_001042"
                     /pseudo
     CDS             complement(10708..11275)
                     /locus_tag="ACG8OO_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(11275..12231)
                     /locus_tag="ACG8OO_001043"
     CDS             complement(11275..12231)
                     /locus_tag="ACG8OO_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9044940.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12231..12887)
                     /locus_tag="ACG8OO_001044"
     CDS             complement(12231..12887)
                     /locus_tag="ACG8OO_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP9044941.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13257..15275
                     /locus_tag="ACG8OO_001045"
     CDS             13257..15275
                     /locus_tag="ACG8OO_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9044942.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15345..15551
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15702..18116
                     /gene="leuS"
                     /locus_tag="ACG8OO_001046"
     CDS             15702..18116
                     /gene="leuS"
                     /locus_tag="ACG8OO_001046"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9044943.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18464..20017
                     /locus_tag="ACG8OO_001047"
     CDS             18464..20017
                     /locus_tag="ACG8OO_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFP9044944.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20398..22182)
                     /locus_tag="ACG8OO_001048"
     CDS             complement(20398..22182)
                     /locus_tag="ACG8OO_001048"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044945.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22197..23918)
                     /locus_tag="ACG8OO_001049"
     CDS             complement(22197..23918)
                     /locus_tag="ACG8OO_001049"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044946.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24018..24779
                     /locus_tag="ACG8OO_001050"
     CDS             24018..24779
                     /locus_tag="ACG8OO_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9044947.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            25746..26014
                     /locus_tag="ACG8OO_001051"
                     /pseudo
     CDS             25746..26014
                     /locus_tag="ACG8OO_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            26686..>28886
                     /locus_tag="ACG8OO_001052"
     CDS             26686..>28886
                     /locus_tag="ACG8OO_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9044948.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFEL"
BASE COUNT         9045 a         5197 c         6155 g         8489 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct tgaaatgcaa agcagtttgc aacatcagac
    11281 gctcaccttt ttccgtacat catattttcc ttggaagcta tctctgcgaa gaagttcttc
    11341 atcaattgca ttcaagactt cccatttttt caagctccac attggtccaa tcaaggaatc
    11401 ccacggcgca tcccctgtca aacgatggat gatgatctct tttggtatca tttccagctg
    11461 gtcgcaaatg acattgacat agtcaggacg gctcattaat tgaaggcgac cttcatgata
    11521 gtcccgcaac attctcgttt tgcgcatcag atgcatcaaa tggagcttga ttccttggat
    11581 atctgaatct aaaatggtgc gtctgacatt ttctttcatc atttctaaac tttcgcctgg
    11641 taatccgttg atcaaatgcg tacaaacacg gatattatgt ttgcgtaatt tagccacgcc
    11701 atctaaataa gtttgataat catgtgctcg gttgatcagt tttgaagttt cttcaaaagt
    11761 tgtttgcaat ccaagttcta cccataaata catccgctcg ttcagttcag caaggtaatc
    11821 aactacttca tctggcagac aatccggtcg tgtaccgact gataatccta cgacacctgg
    11881 taaattgact acttgctcaa atcgttcttt gattatttca actggcgcat gggtgtttgt
    11941 aaaattttgg aaataaacga tgtattgttg gacatgaggc catttttgat gcatcatgtc
    12001 gatttctttc tggaattgga ttggaagagg gtcttcaggt gcaacgatca tgtctcccga
    12061 accagataca ctgcaaaaag tacaaccgcc atgggcaaca gtcccatctc gattcggaca
    12121 gtcaaatcct ccatcgatcg gcaccttgaa aattttcccg ccaaactcat ttcgtaaagc
    12181 gtaattccaa gaatgatatc ttttatttcc ttctgaataa ggaaaagtca ttaaaagtcc
    12241 ttctttctgt aagagttatc tatttgtttt cttttttctc tatacactgg ataagtcagg
    12301 cacagccaag aaagactgag taagtaaccg ccgatgatat cagtaggaaa atgaacgccc
    12361 agataaatgc ggcttatccc gacacttact ataataaaca tcatcagcaa ttgaagcaat
    12421 gtgcgtaagg ttcttttttg aacaaaagct ggaagcagaa atagtaaaga accgtaaaga
    12481 atcatactgc ctgctgcatg tccactaggg aaactgtaac ttgtttcaac tactaagtgc
    12541 gccaatgaag gacgctctcg catgaaaaat agtttgatca ccgggttgat gattccagca
    12601 attacaataa atccagaaac gaaccaaaat gtttctgccc tttttttctt ataccacatc
    12661 cataaagccg cacaagcaaa taaaatgatg acggtaagag gattaccgaa tttagtcata
    12721 aagagttggt agctgttcag atcaggataa gaagctctga caatgcctgt aaaccactga
    12781 tcgaaaccag aaagccacga cagatggaac tttacgatcc agcccaaaat gaaaaacagg
    12841 acaagaaaga agctaccagc cacctgaaat gatgttttat atttcataca attcctcctt
    12901 tgaagcaatg acttattata gcatttttgg tccagcgaaa aaaacggtaa agagataaaa
    12961 ccaatgtatg attcctcttt gaataaccaa tattcttata aaaaaaataa ctgtaatcgg
    13021 aaagacattt tttggtaatg caaatgaaat gttagggaaa taaaatcagt gtttttcgaa
    13081 atccttttaa acaagaacgt agacgtagga agaacttgaa aatgaacaga agttgttcat
    13141 tacggtaaat tacaatatga ttacaaaaaa caacaaagct cttaaataac ttttaataaa
    13201 tattaagaga agttataatt attaataata gataaattga agttggagga tagaagatgg
    13261 aaaacattgc aagaaaagaa cggagacgtt tagaacagac caaacgtttt cgaaaagcaa
    13321 aacgaggggc agcaatcgtg ggaactgcga tggtaggctg ttctgtcgct gccccattag
    13381 tacagccggt tcctgttcaa gcggatgaaa cacctgttca gttcggcgca agaatcaata
    13441 cagcggcatt tatcacagaa attgctactt atgctcaacc aatcgctcaa gccaacgatc
    13501 tatatgcatc agtgatgatc gcacaagcag ttgtagaaag cggttggggc agtagtgcgt
    13561 tatctcaagc accttattat aatctttttg ggataaaagg cagctatcaa ggacaaaccg
    13621 tctacatgga tacgcttgaa tttttaaata atcaatgggt aaataaaaag gagcctttcc
    13681 gacaatatcc ttcttttgct gaatctttca atgacaatgc ctatgtactt agaaatactt
    13741 catttggcaa cggttattac tatgccggta cttggaagag caacacgaaa tcctatacag
    13801 atgcgacagc ttgtttgaca gggcgttatg cgactgatcc tggttacgca ggcaaattaa
    13861 ataatatcat cgcaacttac ggattgaccc aatatgatac accagctagc ggaaacgcag
    13921 gcggtggagc gactacaggt ggcaataata ctggaagtgc aggtagtact ggcggttcaa
    13981 gcaattctgg taatacaaac tcaggttcag caacttcgac cacttatact gtccaatctg
    14041 gtgattctgt ttggggaatc tcaaataaat tcggtatcac aatggatcaa ttgatccaat
    14101 ggaacaacat ccaaaacaat ttcatttatc caggacaaaa actgacaatc aaaggtagcc
    14161 aggaaaatgg ctcttcgacg aataattccg gtaacaatac caattcttca ggaaatgctg
    14221 gtacatcaaa cggaggacaa acaactggtg cgaaatacac tgttcaatct ggtgattctg
    14281 tttggaagat ctcaaatgat cacggaatta cgatggctca attgattgaa tggaacaata
    14341 tcaagaacaa tttcgtttat ccaggacaac agctaatcgt atcaaatggc ggttcttcta
    14401 atgctggagc aaatactggc tcaaatactg ggaatacaag tggctctagt tcgtccagct
    14461 caagttcttc taatacagct tcaggaacga aatatacagt taaagccgga gactctgttt
    14521 ggagcgtctc aaataaatac ggaattacga tggctcagct aatccaatgg aacaatatcc
    14581 aaaataactt tatctaccca ggacaacaac tgactgtttc aaacagtgga tcaacaaaca
    14641 gcacgtcagg taatacaggt tcgtcaacca atacaaataa cagcagttca gttaatactg
    14701 gcagcagttc gtctcaaggc acttataccg tgaaagcagg tgaatctgtt tggagcgtgg
    14761 caaataaaaa cggcatcacg atggatcagt taatcgaatg gaataatatc aaaaacaact
    14821 tcatttatcc aggacagcaa ttgatcgtta aaggtggaac ttcggcaaat acaaatacag
    14881 gttcaactac ttctgcagcg aaaccaaata caccgaatac gtctgctacg acaagtacat
    14941 catccactgg taatacgatg tataccgtga aagcgggcga gtcagtttgg agcgtagcga
    15001 acaaacatca tatcacgatg gatcagttaa tcgaatggaa taatatcaaa aataatttca
    15061 tctatccagg acagcaagtg attgtgaaga aaggttcatc tcaatcaaca aaccaacaat
    15121 cagttactgg ttcgaaaacc tatactgtga aatctggaga atctgtttgg agtgttgctg
    15181 acagtcatgg aattacaatg gctcaactaa tcgaatggaa caatattaag aacaacttta
    15241 tttatccagg tcaaacgttg attgtagcta aataaaatag ctgttcgaag aaataacgat
    15301 tgaattccac aaaaatattt gttactataa ggctacttaa atatgacaac acttgaaaag
    15361 gaagtagtaa aaagactgaa gtttcagaga gcgcatggct ggtgagaatg tgcacggatg
    15421 cttttgaact cgcctttgag ttgcccttgc tgaatacccg agtaagcaag cggcggtgac
    15481 ggccgttaca acgttcgagg ccttttcact aaggtaaaat taggtggtac cgcgtagatt
    15541 acgtcctata aaaagagcaa tctttttata ggattttttt atttcttttg tgaagtttga
    15601 gaaaacaaag caaaaggaaa taaaaataaa cagattctgc cggttttttc gttatattta
    15661 taatagaaac aagtgttttc aatagaacag gaggacagca aatgagttac aatcataaag
    15721 aaattgagaa aaaatggcaa aaatactggg caaaaacgaa tactttcaac acccatgatg
    15781 atcctgaaaa gccaaaattt tacgcactgg atatgtttcc ctatccatcc ggtcaagggc
    15841 tacatgtagg gcatcctgaa ggttatacgg caacggatat cctttcacgt gtgaaaagaa
    15901 gtcaaggata caacgtgctg catccaatgg ggtgggatgc attcggtctg cctgctgaac
    15961 aatatgcgct agatacagga aacgatccag ctgagtttac ccaaaaaaat atcgaaacat
    16021 tccgacgcca aatcaattct ttaggattta gttatgactg gaatcgcgaa gtgaatacaa
    16081 ctgatccgga gtattataaa tggacacaat ggatctttac gaagctttat gaaaaaggtc
    16141 tagcttatga agcagaagta gcggtaaact gggtacctga attaggaaca gttatttcaa
    16201 atgaagaagt gatcgatggg aaaagtgaac gtggtggcta tgatgtagtc agaaaaccaa
    16261 tgcgccaatg gatgctgaaa atcactgctt atgctgatcg cttattggat gatctagaat
    16321 tagttgattg gccagaaagc attaaagaaa tgcaaagaaa ttggatcggt cgttcagtag
    16381 gagcaaatat tgaatttaaa gtggctggta cggataaatc ctataccgta tttacgacac
    16441 gaccagatac tctattcggt gcaacttact cggttcttgc acctgaacta gatcttgtta
    16501 gagaaattac gacccctgaa caaaaagctg ctgtagaagc ttatatcgaa gagacagcta
    16561 aaaaatcaga tctaaagaga acggatcttg caaaagaaaa aacaggagta tttactggtg
    16621 cttatgcaat caatccagtt aatggcaagg aaatcccaat ctggatcgcc gattatgtat
    16681 tatcttcata cggcacagga gccatcatgg ctgttccagc acatgatgaa cgtgactttg
    16741 aattcgctca aactttcgga ctagaaattc ttccagttat cgaaggtgga gatgttgaaa
    16801 aagctgctta cacagaagat ggtacgcaca tcaactctga atttctagac ggcatgaaca
    16861 aacaagaagc aatcgataaa atgaatgaat ggctagaaga aaatggcgta gggaaaaaag
    16921 aagtcagcta tcgcttacgt gactggctat tctcccgtca acgttactgg ggtgaaccaa
    16981 tcccaatcat ccattgggag gacggaacag tcacagctgt cccagaagaa gagctaccat
    17041 tacggttgcc taagacaaaa aacatcaagc caagcggtac tggagaatct ccattagcaa
    17101 atatcgagga gtgggtcaat gtcgttgatc ctgtaacagg taaaaaaggc cgtcgcgaaa
    17161 cgaatacaat gccacaatgg gcaggaagct catggtatta cctgcgttat atcgaccctc
    17221 ataacaaaaa agaactagca gattatgaaa agttgaagcg ttggctacca gttgacatct
    17281 acattggtgg agcagaacat gcggtacttc atttactata tgcacgattc tggcataaat
    17341 tcttatacga tatcggtgtt gttcctacaa aagaaccatt ccaaaaacta tacaatcaag
    17401 gaatgatctt aggcgaaaac aacgaaaaaa tgtcaaaatc tcgtggtaat gtagtcaatc
    17461 ctgatgatgt ggtcgaaact tacggtgcag atacactgcg catgtacgaa atgttcatgg
    17521 ggccattaga tgcttcgatt gcttggagcg aaaatggctt agaaggaagc cgtaaattct
    17581 tggatcgtgt atggcgtttg atcgttgatg agaacaataa aatgcgtgac cgtatcacta
    17641 cattgaatga tggcaagctt gataaagtgt atcatcaaac agtgaaaaaa gtgacagaag
    17701 actatgaaaa tcttcatttc aatacagcaa tctcccaatt gatggtcttt atcaatgaag
    17761 cctataaagt ggacgctctg ccttatgaat atatcgaagg atttgttcaa ctactcgcac
    17821 cgattgctcc tcacatgggt gaagaactat gggcaatcct tggaaatgat ggtggtattt
    17881 cttacgcacc atggccaaca tacgatgaag cagcacttgt tgaagacgaa gtagaagttg
    17941 ttttccaagt aaacggtaaa gttcgtgcga aaagcaatgt tccccgagac ttaggaaaag
    18001 atgagctaga aaaagcagca ttagccaatg aaatagtgca agaatatatt gaaggcaaaa
    18061 cagtcagaaa agttattgcg gtaccaaaca agttagtcaa tatcgttgct aattaattgg
    18121 ctctttgtca ataaggactg atgagttgtg caaaatcaaa tctgggccag aatgaacttc
    18181 attctgtctc agattttttg ttatttcaaa ttgattaatt gattgatcaa aaaggttttg
    18241 attttcatgt tctcaaacac gttcaatgtc tttttcttct gaatggtctt gtaagagatc
    18301 tctgtaatca tcaaattttt gtctattaat cttagtattt tttgatgtat aaactacctg
    18361 aaattgagag tacataccaa attcataaat tttttattag tgggactagt gattgtatgg
    18421 attttgacgc ataatgaatg aagtatgaga aaaggagaga caaatggatc aagaactatt
    18481 tattatcgaa gtattatcag ctaaacaaaa aattatcgag aaagcgaaaa aaagagcgaa
    18541 aagaataatg ttcaagcttt taagcttagt atttttctct ggtttgcttt ttttcggagc
    18601 tgtatcgttc ctatatcgcc atatgagtgc agtagtccgt tcagctgaag ggatgcaaaa
    18661 attaaataat ttgtatcact tttcgtgggg agttggcgga ataacaattg ttgtgcttat
    18721 tgtttttctt ctacttattt ggctccgtta ccatcaattg tcgcgtcatt tcgatcaatg
    18781 gcttagtcaa agagcacaac agtttttaca taataaacca acttctcaag atgatgacta
    18841 tctttatttt gagaagactt cgacgagtca agcttttcgg atacaaaaaa gtgattgcca
    18901 gttattgctg agatcacttg atgatttacc tatctactta ggcgaaagca aatcaaaatt
    18961 cggaatgaac acattccata tttttttact agaaaacgaa cccagatggg aaaaaagcca
    19021 gtcttcgcct ggtggatacc tgacaactaa gatgagtaca ttagtcactg gttttttcct
    19081 cattctttta gctggaacaa tctattatgg gagatacact cagccaaaaa tgtcttctca
    19141 tataaatgct gcttctcaca tgacaatgaa ctcaactaac gatacgcata tacaaaagac
    19201 aaatgaaggg ttgttagctg atcctaagca aaaggtaaat ttgacaacac aaaagataaa
    19261 tgatctcaaa cttgatacag agacgcactc tctctatatg acaacagata gtggtgctaa
    19321 ttgggtattt gtgccgattg attcgcaatg gttgcgtttt ggcgaataca ccctgacaac
    19381 ggggctgatt cctgaaggtt attggatgga caacacatat gatatttccg ccgatttctc
    19441 atggtttatc tattcttctg gggatgatgt acgtctgctg acttcaagag ataatggtaa
    19501 aacatggcaa acgtctatta tcacccaatc agttcagccg atacgttatc gaaaagcggt
    19561 attttatggc aacgatggtc tatttatctg tacatcagcc agtgggatgt ctgcagaaac
    19621 tttgtacatt tacactacta tggatggcgg aatcacttgg ataaaaagtg gaagtacaat
    19681 gatcaatcag ccagtacaaa atgtcagctt cgttacaact tctttaggtt ttgtttcgac
    19741 acgtgaaagt ttgttttata caaacaacgg aggacgctca ttcaaagaat cagttctgtc
    19801 gattccggaa aactatcaga caggggggat agatcttttt caatcaccaa atgaagttac
    19861 tcaaataagc gcgaatacgt tagagacaaa attttatttg ttgaaaaaag gaccaatcga
    19921 tgcagggaaa atgtttgcct gcctcttccg ttcaactgat aacggggaaa catggcaatt
    19981 cgttcagcaa ctatcgcaag tagaacaagg agattaaaga agagagattg tgagcctgac
    20041 gttcagcacc gagtcacaag aaacaaattc aaaaaacagc ttctcatatt tgttgaatag
    20101 tgtaactcgt gattcggagt tgacaagagc aacaagaagc gattttgaag tatagcttct
    20161 cgtattttac aagatcgtgt cttattgccg ataggttgtc tcttgaacac cgtttattcg
    20221 taaaaaggga ttgtgacaag tcttttgtca ctacttaccg aacgaaactg aaggagctat
    20281 ccaaaaagta tcattagtat tattctttgt gtttctagtg gcaccactag tacatttaaa
    20341 aaagctatcg atcatggact aatcatacat gatcgatagc tttttagata atgtttgtta
    20401 cgctatctga aattgactat tataaagact ggcataaagc gttggtgatt ggagtagttc
    20461 agaatgtgtt cctttttcga cgatatcgcc attcttcatt acaaggatca tgtctgcatt
    20521 ttcaatcgtt gacaaacggt gtgcaatcac aaaacttgtc ctatcttttg tcacagcttc
    20581 cattgcttgc tgaatcaatg cttctgttcg tgtatctacg ctagaagttg cttcatctaa
    20641 aataacgaca ggaggattcg ctagaatgac tcgagcaatt gtcaataatt gttgctgtcc
    20701 ttgagataac gtgccgtttt cgctagaaat gatcgtatca tatccgtctg gcagtgttcg
    20761 aataaagtga tcacattgtg cgatttcagc cgcttcgata atttgttctc ttgtggcatc
    20821 cattttcccg taggcgatat tatcagctac cgttccttca aacagccagg tattttgtaa
    20881 aaccatccca aaagaacgac gtagttctcc acgtgaaagc ttggtaatat caagaccgtc
    20941 gaattcgatc tttcctttat tcagttcata aaaacgcatc agcagattga ctaaagtcgt
    21001 ttttcctgca ccagttggac caacgatcgc taccgtttgt tttggcttta ctgaaaaatc
    21061 gacttgattc atcaatattt tatctgatgt gtaaccaaac tgtacatttt tgaaagctat
    21121 ttttcctttc ggttgttcta aaagtcttgc atctggaagg tctggaattt catctgtttc
    21181 atctaatatc tcaaatatcc tatcgattgc tgccattgcg gattggatcg agttgatcac
    21241 ataagaagcg gtagaaattg gttcagaaat ctgattgatg tactgaagat aagcttgtaa
    21301 gaagccgatc gtcacaccac ctgatagaac aagcatagca ccgaataccg cacttatgat
    21361 gaaggcgagt tgattaatga aacgaatggc cggatagata gcacagttca taaattgagc
    21421 acgtttaaaa gcgttgtatt gctttttatt tacttcgtgt atagcagctt ctgcatgagc
    21481 ttgctgattg aatgttttga ccactagatt accagctaag atttcttcca ttcgattatt
    21541 taaaacaccc agttcatact gattctgttc agaaatctgt ttgtttttgt tcgcaatctt
    21601 agttgtaaca attgaactgc caacgatcag caataagacg agcaaagtaa gcttgctttc
    21661 gatataaaac agcatcagac cagcagcagc tattgttacg acagaagtaa aaaattgatt
    21721 gatacctgtc aataacactt gagaaagttg attgagaccg gtagttgtcc gactaatgat
    21781 gtcacctact tgatgattgt caaaaaacga catcggtaat gttttgaatt tttttgtgat
    21841 ttcttttcgc accctcaatg tgatcttttc gcttaacgct gccattgtcc gttcctgaat
    21901 aaatgaagtg atgctgctta agactgaaca gccgatcagc aataagacag ggaacaacaa
    21961 tgcttgtttg atatcactga aagaaagctg tccaatacct gtttttctta tttctgataa
    22021 gagatcatcg atcccgattc ccatgatcaa gggcatggct acgactaata ggttcccgaa
    22081 taaactgcat agaaacaatc cgtaaaaata tttatgttca ggtttgatca ttttccaaaa
    22141 acgtatcaaa gcacgcgtgt tcaacgtttt tttcttttcc atcgtatccc ttctttctat
    22201 attccttgtg attttgcaaa ctcctgataa gtttggcaat tagacagtag ttcttcatga
    22261 gtaccttgtc caacgatttt tccttcatct aaaacaatga tgttatctgc ttgttggatt
    22321 gtactgaggc gttgagcaac aatcacgaga gttttctccg acatgtgttt atgaagggct
    22381 gctcttaatg ctgcatctgt tttatagtct aaagcggaga aactatcatc gaatacatag
    22441 atctctgctg gtttgatcaa cgctcgagca atacatatcc gctgtttctg gccccctgaa
    22501 taattagaac cgccttgagc aacaaagctg tccaatccgt caggaagggt gtccactagt
    22561 tcttctaatt gagcgatctt gatcgcttta gacaactctt ctgttgaggc atattcattt
    22621 cccatctgca agttgcttaa aattgtcccg ctgaataaga aagccttctg agggacataa
    22681 ctgatgtggg agcggatctg ttcttgagtc atttgagtaa gaggaatacc accaagggtt
    22741 acttttccta tcgtagggtc actaagtctc aagagaagtt tcgccaaact actttttcca
    22801 gatccggtcc cgccaacgat cgctgttgtt tttcctttag gtattgtgaa atcaatatct
    22861 tctagaactg gatcagagat ttcagtatat ttaaatgtga catgttcaaa agcagctgca
    22921 tttgtctgat caaaggttgg ttcagctact gttttgatcg tctcttcctc aatttcgatc
    22981 tctgcttgca gtatttcttc tatccgtcgt aatgaggcaa acgatttagg gataatgacc
    23041 aataccatag ctgcgatcat caagtaagca agtgtcagca tagagtattc gactacagca
    23101 gtcacgacac cgatttgtaa tgctccttct gctacaagat ttccgccaaa ccacaaaata
    23161 gaagcataaa cgattcccat gagcaaaaaa gcaattggag tgacccaagc aaatattcgg
    23221 ttcactttga tcatttgatt agcatagtca gcaaagctct tattcgttcg cttttcttca
    23281 tatgattgat tatcaaaggc acggatcata ttaatcccag taaagaattc tctcaatgtg
    23341 atggtgatcc gatccatttt tggctggata ctcaaagata aaggtgcccc tcgtttcatc
    23401 agtcgataaa caatccatac ataaaaaaca acagagatca atgtaaccca tgttagtaat
    23461 agagaataac gaaacatcat gagaatggtg aaaacgcaaa taaccggtgc tggtaagatc
    23521 atttggcaaa ataaaacaat catttgctgg acattatcga catcattcgt cattcttgtc
    23581 aaaagagatc cagaactaaa acgactgata tccttcattg acagctgttg gaatttacgg
    23641 aaatatttct cccgcgtttg gaatccaaac ctagcggaag ccaatgctga aaagtaacta
    23701 gaggcaactg cagcgattgt tcctagaaac gctacaagca gcatttcaac tccaattttt
    23761 tttataactg aggcttcgcc acttgctata cctgtgtcga tcagctttgc tactaatttt
    23821 ggtacaccta atgtaccggc gatttctaaa cagatcatca caaaggtaag gagagcgagt
    23881 cctttgtttt tctgtaagaa tgtttttaag atagacatat tattcctcct tttttcctga
    23941 tacttttcga ttctatacct tcaagtaact tgaaggtcaa tagaaaaaag aataaactga
    24001 aaaagaggtg agaaaaaatg aaaaaaatgt atacgattgg ccaagtatcg gaactatttg
    24061 atttgccaaa atcaacgatc cgctattggg atgaacaagg attaatccgt tcaagtagac
    24121 aagaagagaa tgattatcga ttgtttgata tcgatgatat ttttatgctt tatgatatcg
    24181 ttttttatag aaaattagat ataccgatca aacagatgaa gaatttatat ggaaaaacat
    24241 tgacggagtt atatgagaca cttgatgaaa cagaaagaag aatccataaa gaactggtgg
    24301 tgatgaaaca aaaacaaaaa gagataaggg agcaaaaaaa acagttgaaa ttgatgatcg
    24361 atacaaacga ggaagaattt cctgtagaag agatcccttt tgactgtatg atctcaacgg
    24421 agtttgaaga tattgtggaa atcaaaaaat ttctacctaa ctattcaagc tttgggatga
    24481 tgagtatgcc agcttccagt tcacagacga tgtatggatt ttttatcgac cctagtgaag
    24541 tacatttatt tactcaagac gtgatttggg agaaaaaaga tacggctgtt tatcgtcgat
    24601 tcttattgaa atccgagatg aaccatgcgg aacgtaataa tattttagaa atcagagaaa
    24661 gaatgtatga aaaaggatgg aaaacaggag aagtgatcgg ccagtatcta ttgacgaata
    24721 cggatgaaaa caatatcaga actgaatatt atcatgcatg gatagaaatg aaaaaatagg
    24781 aaatatgtgt ttaagataac tcgaaaatga tggttgtttt agttataaag aaaaattttc
    24841 tggttcgatt acgggtttaa tgtgggggat tattagtcgt ggatagatag aattttagtt
    24901 ttttagagga aatatgtctt taaaaatgga atggttgcaa ttcttagcga tatatacgat
    24961 gtaattgtcc tcttaaaaaa gtatggaaaa ataattgtat tgggtgttat gtataattca
    25021 tggtgaactg agactaatga gaagaaaaat tcatattgtt atgagtgaga ttgtatcaga
    25081 tgagaatgat aaattaaata ggtgcataaa agcattacac ttaaaataca ttgttgctta
    25141 cattattaca atatgttaat aaggcaaaaa taaatagatg gattgaataa acatattttt
    25201 taatcaaata gtccctactg tgactttcct cagctatcgg tgatactacc gatagcattt
    25261 ttttgcttat aaaataatta taagtattta gagatttttc ttctaacatt cttcaaacaa
    25321 gaaaaatcac aaaggttatt gttattttta tatattcttt ttaagaatga ttgttttttt
    25381 atagaaattt taaaaaataa aacacgttat tgatggatag ttagtttgtt atttggtaat
    25441 attatagaag ttgactggta gtgatttatt tttcgtcgtt ttataaaagt gttatttttt
    25501 ggaaaaattc acaaatatgt aacgaatgtt tttctggaat ttgctgtgtt aaaaagcagt
    25561 atgactaata taaatactat tgtcatttat gcaatttttt atggatagta attttagagg
    25621 ggaatcaatg aaggaagaat agaagggaaa aatagttgca aatacgccag ttagcaaaaa
    25681 tagcaataca tcttcaagct ttaaaattcg agagaatcat cttggtagtg gaatcaacta
    25741 caacaaaagg attaaaccaa ttaatagata gaattaccaa tggaaaaata gaaaaggtag
    25801 ttattctata caaaagttgt ttaattcgct tcggttatga acttagtgaa accctttgca
    25861 atatatggta cagaatatag aaattattga cattacagaa aatgatgaag atttaataca
    25921 gattgtcact atttgtagat accttttaca aggaaaccga gccaataaag ctaagaagat
    25981 gattaaggag ttgatggaag acgatacaag ttaagaaagt gaaactacgc ccaaataaag
    26041 agcaaaaaga gtaattggac gtggaaaatg caagaaatga accatcgatt cggtggacag
    26101 tttattagta atgacggttt gagaacatcc atgacgagaa tgaaggaaca tcctaaatgc
    26161 gtttagttga accaagtgcc aaataatatg gccaaataag cgatcagtta tcgatgaatc
    26221 ccaagtattc taaactatat attcattttg atgctaagca ttgatatatt tttttgggat
    26281 tgatgaaggt taaccaatac agggattcac tgatgaaatt ctagagtctg accttggtat
    26341 caacgattta gtgattgttt ctaatggaaa agtataaaag tatataagta cattaacaaa
    26401 ataactatta gggttacgct ccagcttgtg caaggcactg gcaggaggaa tcgtgaaatc
    26461 ataattatgg ttaagccaca cggacaacta aaagtcatac aaagctatcg aatcaatcaa
    26521 cagaagacaa tcggcaatca ttagctttgt ttacagctta cattgcaacg cttgagttga
    26581 accgaaaata cgagggaggt aacgcagcca ttttgagttg gggaatcttt ttagaaaatt
    26641 atcgggtacc ttgttgttta gtcaaaatta taagggggag taatagtgag gaacaaattt
    26701 cataaactgt taagtatgtt tctagttatg cttattgtga tcaatcaatt tgggaacata
    26761 acggttctag ctgagtcttt agcagaacag ccagagcgtt cgctgacgat cgaagttgat
    26821 caagaggagg ctgctttggg tgagcagttc cagttgcgag tcagaggaac accagacctg
    26881 ctaaatgcat tggtcattga taagagtgag gatttgattg aaggggaagt gaagcaactc
    26941 aatgattacg aacgtctgat tacttattct tcccagcatt caggtaaata cgaattgacc
    27001 gcaagaagcg gtgatattca atcaaatacg gtctcaatag aaatcagtgt accagaagaa
    27061 agcacaagtg agtcttttaa tgtaccagaa gaaagcacaa gtgaatcttt taatatacca
    27121 gaagaaagct taagtgaatc ttttaatgaa gccagatcat ctgagccatc tataacgcag
    27181 gaatctcaga ttgaaagaca agaggagctc ttgaattatg acatcggcat ctctgctgag
    27241 cgccccactt tgccgagcag gcatacacaa acgttaaagg gatacattca aggaggcgct
    27301 ggcgatattc ttgaagaata tggggagatg gtcgtacgag ttcctagagc atcgattggc
    27361 ttaattgagt tcattagagt ggaggtcgct gatgattcac ctttaaagcc actggcattt
    27421 taccaagaag gcacagacgt tgtttttcca tttacctttg attatgaagg attaaatgaa
    27481 gccgctcagt cgtatacatt cagtatcacg tttgaagttg ctttctttca atcaggcggt
    27541 actgagccaa ataatgactt tcaagtgagt cttacttatg ctgagcgagc agcaaacacg
    27601 gaattttcaa ttacgcctat tccttcatca agacccattt tttcgaaatt atactatggc
    27661 tctagagaaa tcgacagcca aggaaatctt gggaatctgc tgattgacgg gcaagacgtg
    27721 accaacagta ataaatttat tttgcaggta aattatgaag gatcacgaaa agacggtgta
    27781 gtggttaccg atgtactacc gcaaggaaca actttggcac cgaatccgac tacatggagt
    27841 tccactggaa atagagaacc tatttcaggt ggggttcgga tcgcagaagt aacgaatcca
    27901 ggtgaagcaa acgttagaac tcattatgtt tccgaggaat ttgtggacaa agtatctttt
    27961 aataaggaaa cgaatacact tgttgtggac tttggtaatc tagcagaggg tcaatcgtat
    28021 tttattgaat atgctcttga agtgaccgat gtcgacttgt tcaatgaagg aagacaagag
    28081 gatgggtttt ctcggaatcg agcgagtatg acctacaata atcaggtaat gagccgagac
    28141 taccgcttga gaatccctga aagtaatctt ccggcaatct ctttaagaaa aagtgtggac
    28201 aaaaatgtta tcaatctcaa tgaaaatgaa ctaacgtata ctctgcatgt ctcgatgttt
    28261 tctggaacaa gaaatacgct tgttattgaa gatgttttgg aagaaggcat ggaatttgtg
    28321 tcatttctat cagatattcc ctctttcatt gatgccgaag tcaataatgg aatagttcgt
    28381 ttcagtgcga ataatacgat tggtggtccg acaagtttta ccctctcttt taaggtcaat
    28441 gtagaggcgt atgcggtcgg cgatcaaatt ttaaactttg caacgcttgt gaatagtgac
    28501 aatcgattga atacgagtac tgtaagaaca aggaaaatcg atgggcgtat taaagtaatt
    28561 aaaactgatg agaatggcca aaggctggca ggagcaaaat ttgaaatttt atcaagcgat
    28621 tcacaagtga tacaagaagg tataacggca gaaaacggag aatttttaag tgaaccgctt
    28681 cctgtaggta gctatcaagt ccgagaaaca gaagcaccgg aagggtatct tttagatgcg
    28741 acggtacatg atgtttttat aaatgaatac tccatagaag aagtgacgtt aaccattgaa
    28801 aacatccttg atacgggtgg tgttgaacta attaaacatg ctgaaggcag tggtgaagtc
    28861 cttcaaggag ctgtttttga gttaca
//
LOCUS       DAZKBH010000024        27837 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000024 DAZKBH010000000
VERSION     DAZKBH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.024"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..1164)
                     /locus_tag="ACG8OO_001053"
     CDS             complement(220..1164)
                     /locus_tag="ACG8OO_001053"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9044949.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1460..2404)
                     /locus_tag="ACG8OO_001054"
     CDS             complement(1460..2404)
                     /locus_tag="ACG8OO_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9044950.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2625..3839
                     /locus_tag="ACG8OO_001055"
     CDS             2625..3839
                     /locus_tag="ACG8OO_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9044951.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3861..5876
                     /locus_tag="ACG8OO_001056"
     CDS             3861..5876
                     /locus_tag="ACG8OO_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFP9044952.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5959..7332)
                     /locus_tag="ACG8OO_001057"
     CDS             complement(5959..7332)
                     /locus_tag="ACG8OO_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9044953.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7358..7642)
                     /locus_tag="ACG8OO_001058"
     CDS             complement(7358..7642)
                     /locus_tag="ACG8OO_001058"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9044954.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7644..9659)
                     /locus_tag="ACG8OO_001059"
     CDS             complement(7644..9659)
                     /locus_tag="ACG8OO_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9044955.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9838..10140)
                     /locus_tag="ACG8OO_001060"
     CDS             complement(9838..10140)
                     /locus_tag="ACG8OO_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFP9044956.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10229..11260)
                     /locus_tag="ACG8OO_001061"
     CDS             complement(10229..11260)
                     /locus_tag="ACG8OO_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9044957.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11467..12342
                     /locus_tag="ACG8OO_001062"
     CDS             11467..12342
                     /locus_tag="ACG8OO_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9044958.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12494..13336)
                     /locus_tag="ACG8OO_001063"
     CDS             complement(12494..13336)
                     /locus_tag="ACG8OO_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9044959.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13428..15305)
                     /locus_tag="ACG8OO_001064"
     CDS             complement(13428..15305)
                     /locus_tag="ACG8OO_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9044960.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15302..16723)
                     /locus_tag="ACG8OO_001065"
     CDS             complement(15302..16723)
                     /locus_tag="ACG8OO_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9044961.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16726..20115)
                     /locus_tag="ACG8OO_001066"
     CDS             complement(16726..20115)
                     /locus_tag="ACG8OO_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9044962.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20372..21763
                     /locus_tag="ACG8OO_001067"
     CDS             20372..21763
                     /locus_tag="ACG8OO_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9044963.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21836..22483)
                     /locus_tag="ACG8OO_001068"
     CDS             complement(21836..22483)
                     /locus_tag="ACG8OO_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9044964.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22491..23177)
                     /locus_tag="ACG8OO_001069"
     CDS             complement(22491..23177)
                     /locus_tag="ACG8OO_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9044965.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23183..24256)
                     /locus_tag="ACG8OO_001070"
     CDS             complement(23183..24256)
                     /locus_tag="ACG8OO_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9044966.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24478..25152
                     /locus_tag="ACG8OO_001071"
     CDS             24478..25152
                     /locus_tag="ACG8OO_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9044967.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25157..26497
                     /locus_tag="ACG8OO_001072"
     CDS             25157..26497
                     /locus_tag="ACG8OO_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9044968.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26646..27632)
                     /locus_tag="ACG8OO_001073"
     CDS             complement(26646..27632)
                     /locus_tag="ACG8OO_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFP9044969.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8125 a         5249 c         5046 g         9417 t
ORIGIN      
        1 ggatttttta attaaccaca gataaacatt gataacgatt cgcttattgt aatcaacaga
       61 ttaaatttca tatgttcata tgtacggata cattttcgga ccgattaatt ttaaccattc
      121 tgtttatttg cttacctatt agatatcaat aaaaaactgc ttaacagcta tttcagctgt
      181 taagcagttt taggattttt agttcatatt cattgtttct tataatacag tgttcaatac
      241 ttctcttaaa gcacttgttg atttgctgaa cattgctttt tcttcttcgt tgattgataa
      301 ttcaacaact tcacgaacac cattttcgtc aacgattgat ggcacacctg taaagatatc
      361 tttttcgtcg tattcaccag ttaaataagc agatacaggc aatacagctt gttcgttatt
      421 caagatagct tttacgatac gtgcagtact cataccaata ccatagtaag ttgctttttt
      481 acgatcaatg atctcataag cagcattacg gactttatca gcaatcacat ctaattcatc
      541 ttgggcaata cgatggtctt tttctacaat ttcaaacact ggtttaccac cgacagttgt
      601 atgtgaccat gcagcaactt ctgaatcacc atgttcacct aagatatagc cgtggacgct
      661 gcgtgggtca acttttaatt tcaatgcgat ttctttacgg aaacgagttg tatccaatgt
      721 tgttccagta ccgataacac gtgatgttgg cagaccagat tcgttccaag cgatatatgt
      781 caaaatatct actgggtttg aagcaacaac gataataccg tcgaatcctg atcccatgat
      841 ttcttttacg atttggcgca tgattgacgc atttgtttta actaaatcta aacgagattg
      901 accaggtttt tggttgatac cagctgtgat aacaacgata tcagcatctt tacattcttc
      961 atagccacct gaccatacag ccacattttc atcgccccaa gccataccat ctaaaaggtc
     1021 caatgcttcc ccttctgctt tttcttgatt cacatcgatc aatactaatt cattagagat
     1081 tccttggttg atcattgcat aagcgatact tgttccaaca aaacctgtac cgacgattac
     1141 tactttacga cttgtttttt tcatgaataa acagctcctt ttcataacta tttgttactt
     1201 tcatgaaaag tatagtcttg tttattcatt ttatcaacat gttaattata atagttgtta
     1261 ttataatttt tgatggaaac gaattcctga atacgcataa cagataaatg acaaatgttt
     1321 acacctatat cccctgataa tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa
     1381 ttcgctgcaa cgatataata agacagcaat cgcacctaaa acaattttcc ttcatatgct
     1441 ttagtgaaag attttcttct taatgtttat ttctccattc attgggagta catgttgaat
     1501 acttcttgaa taatttgaat aaagaagtcg tttgtttata tccgatacga ttagcgattt
     1561 cttccaacga aaaactcgtt tcttgaatca gttccaacgc aaagtcgaat tttttcttta
     1621 gaaccagttc ttgaaaggaa gcatcaaaat acattttcaa tttattacta agatagaccg
     1681 gattgtagcc aaaatgagct gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga
     1741 tataatctaa tactctaagc atctcatttt ctgcgcatcc atctgctgat gaaaagcttt
     1801 gttgttctaa taaatgagtc aattcgatca gtaatagtga tgtatagaga tttaacgaac
     1861 ggatcttatg actgtatgta tcaaaataaa tcaacagcat attttcaatc aaatgcgtca
     1921 aacgacttgt ttttccgccg cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg
     1981 atgcatttat caaaaaagaa gaaagtagat tatttgtgct tgccaaatga ttcaatatct
     2041 ctgtagataa ggtttcttct tttaataaga tgttgattaa aatatcctct tgccctgtgt
     2101 attcgattct ttgagtagct tctttatcaa tgatcaccac gtcactttct tccatctcta
     2161 tcctttctcc attaataatt tgtaaacatg taccgctata catataattg atttcaacaa
     2221 aatcatgtgt atgctcggga acttccgcat atcgacggtg tttactgata tgaatatttc
     2281 cctgttgaaa tagatcccct cggtggattt ttggcggtgt ctgaagatgg taagctaagt
     2341 ttaaatcatt gatattcatt ccttgttcac gctgcagagt ttcgattgat tcttcgttac
     2401 gcaaaaaaaa tgtctaattc agctagttcc aattgactcc ctcctttttg taagcggttt
     2461 ctttcgctta ttagttcacg ctgtactgct attatatgca aagctatttt tggtttcata
     2521 aagactttct atggttggga catgaacttc ctgtgattcc gctattgaga gaataaccac
     2581 ttcatactat atttttgata agcgcttaca aaaatgaaag gaacatgctt atgaataaaa
     2641 agatgatgaa attttctctg ctgtcagtct ctcttatttt gacttctgct ggtgcagtca
     2701 cgtctgcaat tcccagtatg tcagctactt atgccacgcg ttctttatct gctgttgaat
     2761 tattaacgac tgctccctct tttatggtca ctatttttgt tttgattagt agttttattg
     2821 ctaggaaaat cggacaaaaa caaactgtga tcatcggatt agtgattgca tcgatttgtg
     2881 gtatcctccc cctcttctca caaagttaca gtattgtctt agcttcaaga gctggactag
     2941 gtattggctt tggattgatc aattctttgg cagtcagcat gattgctgcc ttttatcacg
     3001 gagatgaacg agcctctatg attggttttc aaagtgcatt tcaaggattt ggtgcagctt
     3061 taatgacatt tgttgcagga cagctagtac agttcggttg gcaatatacc tttttagtct
     3121 acgccatcag cttacctatt cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa
     3181 ctgaacttca atcgcatggc acagccccaa caagacaagt aatcaaaacg aatcctaaaa
     3241 tgattagtta tggtattttt ctcttgctgg tcatcgtggt atataatgct gtatcgatta
     3301 aattagccac agtcctgacg acttattctc taggaaacga aactaatgca gcgaatatct
     3361 taacaatcat gcaattggca agtatggctg ccggagtttg ttttggctgg cttcagacaa
     3421 aagcacatcg ctatatgctt acactttctt tactactgat gggaatcggc tttgtattga
     3481 ttgccactgt gataaacctg tatgtgattg ggtttggtgc gatcctaaca ggaatttctt
     3541 tttcgttatt cattccttac ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg
     3601 agagtttcaa tacttcaatc ttattggttg gcgctaattt aggtactttc attgcaccat
     3661 atggtttgct ctttctctct tttgcttctc ttgggagtaa aacacttgct gttttcgtca
     3721 atggcagtat cttgctgttg attagcgcgc ttattagcct tctagtagta cacaggacga
     3781 atagaaagac agtgataaga gtagatgaag aaataaaaga acaattgaca acaaaataaa
     3841 tttgatggag gtcgtgtaaa atgacaaatg aaaaacataa agatagatgg ctttggtatc
     3901 ctggagactt tgagatccgc catggactgc tacaaaattt ccaacgggaa gaacgaggtt
     3961 ttgactggcc agcgtactgg tacatggatg attgccatcg gaacgtcaag tttaagagat
     4021 attactttct tgatcaacct tccatgttta aagtgacgat ccaaggagtt gggtatgtag
     4081 aaattaatgg acaaaaacat ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa
     4141 aaattcgtat ctttgtagga catacatcag ggctaccagc gatgtttatc gagggagacg
     4201 aagtaaaatc agatattggc tggacagcaa gtaattttat tgaagaatat ccagctggct
     4261 ggagtccgct ttatgtcgat atcgcaaaag atccaaatca aatctattat caaaaagaat
     4321 gcgtccatcc agtcactgaa acggagattg gcggaggaat cttattcgat tttgggcgag
     4381 cagtgaatgg ctgtgtagaa ctaatatctc tggatgacca tatcgaatct atcactctat
     4441 gttatggaga gtccgatgta gaagctatgg atatcgatta ttgctactat aagcaaatag
     4501 gaatgacaag aaaaatgaaa gctcgtaaac gcgcttttcg ctatttgttt ataccagagg
     4561 tttctactgg aatggttcaa cttcaagttt ttcatgaatt tttatctctt tcgaaacaag
     4621 ctgctttttc aagcgattct tctttactga atcagatctg gtcagtatca gaagaagcct
     4681 tcaagttatg tagtgatttg ttttttatcg acggtatcaa acgagatcgc tggatttggt
     4741 ctggagatgc gtatcaatca ttcatgatca atcaatatct tttttttgac gaggaaatca
     4801 ataaacgaac gatattagca ttaagaggac aaaatgaaat caagcagcat atgaatacta
     4861 tcgttgacta ttcgatcttg tggttgattg gtatcgaaaa ccactatatg atgacgaaag
     4921 actatgaatt tttacgcatc gtctacccta agatgcaatc attaatgaga tacttgatgg
     4981 agcaaacgaa tgaattaggg tttatttatg ggagagcaaa agattggata tttattgatt
     5041 ggtctgaaat ggataaagaa ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct
     5101 atcattccat gatcacttgc ggagatatct tagctgaaac atatggagag aacgaaaatt
     5161 atcatactca tctctatcaa gaaaaatatc aactattgaa acaaaaccta tttgactact
     5221 tttgggatga agaacttggc gcttttatcg attgctatga atctgggaga agaaatgtga
     5281 cgcgtcatgc gaatattttc gctgttttat tcgattttgc taacaaagac caacagcaat
     5341 cgatcttaaa gaatgtctta ttgaatgaca ctatcacaca gattactact ccatatttca
     5401 aattctatga acaagatgct ttatgcaaac tcggacagac gaatatcgtc taccaaacga
     5461 ttcttgatta ttggggaggg atgttatctc atgatgcggt aactttctgg gaagaatacg
     5521 accctactca aacaggaaat gaaaagtaca gtatgtatgg agatccttat ggaaaaagct
     5581 tatgccatgc atggggagca agcccaatct atcttttagg acgctatttt atcggtttgt
     5641 atccaacaaa gccaggttat gaagaattta tgatacaacc taatctagat tcatttgaaa
     5701 cactgaattg tacgctacct atcaaagatg gaaagatttc gcttgaaaaa acaaaagata
     5761 agatcgtgat tacttctgat agaacaggcg gaacagtgaa aatgaatggt aaaagttatc
     5821 ctcttctagc aaaacagcca cttgtcttaa caagctattc aaaaataaat gcataaaaca
     5881 aaaaactgct tgcgacatat cagaagactt taaatcttct tctgatattg tccaagcaat
     5941 ttttattttg aagtttgctt aattctgttc tttagaaaca atgtttgttc tttttccggc
     6001 aaaaagaaag ctagataata cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc
     6061 tatttgaccg tattctccta gtttcccaag tacaagtcct ggtactagat aatcagtatc
     6121 ggcaaatgta gaagaagcga ttttaagatc tcctaatact ggaaggataa ataatggcag
     6181 ccaactaatc aataagctat tgaaagcaga acctaatacc gcccctcttc taccgccagt
     6241 cgagttgccg aagacaccag aagcagctcc gcagaagaaa tgtcctacta ccccaggaat
     6301 gataactgta gtttgcaagc ctatcatagc aaacatggat agtgttccta caaagaagct
     6361 aacaaaaaag ccaattaata cagcattagg tgcatatgga aaaacgatag gaacatctaa
     6421 tgctggttta gaatcgggta cgagtttttt agcaatccct tggaatgctg gaacgatttc
     6481 tgccaacacc attcgaaccc cttgaaggac gataacaaac ccagctgtga atgttccagc
     6541 ttggatcagt gcaaagatta cagcgttgtc tccattgctt agttctcttt caacaaatcc
     6601 aggaccagct aacaaagcaa gtgcgacata tacgatcgac atcaagagca tgatcgatac
     6661 agtagaatct cgcaaaaagc tgaaactttt aggaatcttt atgtcctctg tagaacggtc
     6721 tttgtttcca aaaaactttc cgatccatcc actagaagca taaccgatat ttccggtatg
     6781 ccctaacgca acttgattgt ttccagtgat gttcttcata aacggttgac aaagagcagg
     6841 cgtaatagtt aatagtgttc cttcgaagac cccgcctaat aagatcatcc acaaatttgc
     6901 agtaaacccc gcgcttacta aaatgactgc tgttagacaa gaaacataaa gcattgcttg
     6961 acctgtaaga aaaatgaatt tatatcttgt gaatctagca agtaaaatat tcacgatcat
     7021 ccctactagc atgatcaaag cagtaggtgt accgtattgt tgcaaagcaa tcgccactac
     7081 tgcttcattg ctaggaacta ctccttgagt atgcagagct gtttggaaca tcgaagcaaa
     7141 aggggttagt gaattactga ttaaaccagc accgccgatc agtactagga atccgacaaa
     7201 tgttttgata gttccttgga tcacttctga aaatttcttc ttctgaagaa cgagaccaag
     7261 cagtgcaatc aaagaaacga ggatggaggg ctgactgacg attgacacaa acaaattcaa
     7321 caatttatcc attgatagtt cctcccttga atcattttta ttcttttgac aagatcttcg
     7381 ttactttttc ttttacttct tcgctgtcaa tgatcgattg caaaggtacg agcttttcat
     7441 caggtataga agttagcgta tctttcaaag aatgctcaat gaagaaataa tcagccatat
     7501 cgctagatac acttccaaca tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa
     7561 gatctgcaag tgcttgtttg atgttcatct ctaccataaa acttgttcct aatccacttt
     7621 gacatactgc aaaaattttc atatcattcg tctccttttc ttatgatcgc taatatttct
     7681 tcttttgttg atgcgtgtaa taaatcttga agattttgtt tattagaaag tagctttgtt
     7741 aaatttgcca aagcccgaag atgtgcctca ttatcgatag cagctaaaca gatgaacaaa
     7801 ctaatgggat gggctggatt atcaagcaga tccacaggtt gttgaagttt cagcaaagac
     7861 atccctactt cgtttacgcc atcttctgga cgagcatgag gtaatgcgat gtgatcgcca
     7921 atatggataa acgcaccata ctgctccacg cgctcgatca tagctttgat ataactttct
     7981 tctattttat tttctgataa tagcggctgt gcggcaaatg tgattgcttc ttgccagttt
     8041 tttggttgat ccgataattg gatcatttct tctgttaata attcagatag cataggacgt
     8101 tgatcctcct tcttttctaa aagtttgttc attttacgat ttaatacttg atagagcttt
     8161 tcttcgtcta cgccttcttt taattcgata taaggcagta gcgccgacaa caattcttgg
     8221 gcagaaggca ttgaaaatcc tggtatcagc caatctcttt gtacttgatt gatcagctga
     8281 tttttttcca agtcagtcat gattggatgc agaacataga gagacttttt cttatcagta
     8341 agtgtgagag gtacagtaga aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag
     8401 cggtcagctg aattagtctc tgtaaaaaag atcatcggga ataattgctg aagttcactt
     8461 tttaagatca atgaagagct aatgccactt ggacagacga tcacggctct tatttttcta
     8521 tttctttctt ctgcgtccgc ttttctgatc tctccgccaa atagtatagt gaaaaaacca
     8581 atctcttctt cagggatgct cttccctatt cgtctttcta acggaagtaa tgcttttctt
     8641 gtcatttcgt agatggaagc atattgatgc ttaatttgat cgatcatcac attaggtaaa
     8701 taaaaagaat aggaaatacg aaaataggca gggaccaagt gataaaagac attcatcaat
     8761 aattcacgaa acgaagcaaa ttctacggcc atcaggaatt ccatgcgatg gatgacttct
     8821 gaagaacatt ccagcaaaaa atcaagtgca ggctcctgga catctccttc aacgatcgat
     8881 gctaagcaaa gagagaaaaa tataatctcc gaattttgat gttcggaata gccaaggcca
     8941 gtcaatactt gtcgactaat gtcagtgact gcaagagaag ccaacagttc agcatctttt
     9001 accagttcca tggatgtttt tccaattcga atggttgtca aactcaaata ataagctaat
     9061 tcatccattc gatttggtgc gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa
     9121 tctctaactt tggcgtaata atcaatggaa agggttgcgg ttaaatacgt gagcccaaat
     9181 cttccagcaa gtgactgcat caaacgagca gtgcgatttt ttgcttctct tcttaatatt
     9241 gcttctgacc ctagcagcct aaaaccagtt ttcctagtat acgacagctg acaatcttcc
     9301 acttctaatt catttcttat ctttttgata tctgacagga ctgtcccttt agaaacctga
     9361 agaaaatctt gaaaatgaaa aacagaaaca gacgttgcag tcgaataaac catcaaataa
     9421 agaagatctc ttcgctcttg ttcggtatac acagcttcaa atcgagtaat atccaaaaat
     9481 ttttctgccc agtcgattgc tcggaatttc tttatacgga tacgggtatc atccacatat
     9541 tcggcaaaag gagttaattg tttgatcgcc tcgcttgtat ttacttcgat gatctctgct
     9601 gctttttcta gactgatttc tcttggtgtt ttcaggagat aactaatttt gtagttcaca
     9661 gcgctgttca cctcctactt atattctagt atggtagcgc ttttaaatcg aggggtgttt
     9721 ggtacaactt acagttggat tttttttacg actgtaccga acgatttcta tcgaatggat
     9781 acagaaaaaa agcaggtgac caaatgtctc ctgcttttat gaattgattt cgtccgatca
     9841 atgatgatca tgtttatttt ttgaggtcag ttcttcaaat ttttctttga catcttcaac
     9901 cgctttttcg atatcttttt caaagtgatt gggctcatta ggctttgttt cctcttctac
     9961 agaatttgaa ccagataaag ctgtttcttt attttctgtt tcaccagagt ttgtgccagc
    10021 taatgattct tctttgtctt ctgtttcacc agaattagtt cctgctaaag ccacttcttc
    10081 tgttttaggg gccattgaac ctcttaatcg tttgtgtttt tcaccgtcat aatttgtcat
    10141 gttggttcct cctagtagtt ctatactttt tcatcgtaca ataagacagt ttatgcgtca
    10201 aaaaacaaac ccgattctta caaatatgtc atatttttcc ttctgattct tgaatgcttt
    10261 tgattccttc ttccaaaatt ttcatttgta agattgtttc ttcatctttg atgacttttt
    10321 ctgcgccatt aactaatgtc tcgtaaatcc cttcgtagac acggctgtaa tcaccaacct
    10381 cggaaattac tttttcttca tgataactcc cttgttcatc tatataggtc aatacaccat
    10441 aatgttcggg caaatcgata ccaaagtctt catgatcagg catgtaaaac attttcagat
    10501 gttcttcttg tcgatctttt gtttgtttca cgaacatccc tttttttccg taaacgacga
    10561 aacttggacg ttctttgatg cggaaataac tggattttat cgaaattttc atatttccat
    10621 agaaaaaatc cagatcgaag taatcattca ttcgtccttt ccctaaaagt tgacgtacat
    10681 catagctgat atcatctggt tcgccgaaat aagaaagaac ttgatctact gtatgacaag
    10741 catgtccata taaatagctg ttcgcagtag aaaattcatg cgtattttct ggtacttccg
    10801 gtctgaaata atcataatgc atttctactt ctaaaatatc ccctaatttt ccgctttcga
    10861 ttactttttg gactgtcaag aagtcagaat cgaaccgtct gttttggtaa cattggataa
    10921 atagaccttt ttcttttgct aatgcaaata actcttctgc ttcctttgac gtttctgcaa
    10981 aaggtttctc tactagtaca ttcttaccat gttcgagtgc catttttcca tattcataat
    11041 gaaattgact aggtgtagtg atgacaacta attggatctc aggatcatcc caaagatcag
    11101 acagctgatc agtatattcg atgtcttcga atgttttcca cactggttca gatggggaaa
    11161 aaattttttt gatattgatt ttgttcgtaa gttttcttga aaaaggcaaa tgatagcgat
    11221 ttgtactctt tccattgcca agataagcga ttgtaagcat gagtggtgcc tccttttatt
    11281 gatgttttca gtatatccta gaaaagcgca gaaagtattt ataatagcta ttctggtaaa
    11341 aaagttcaga aaaacacttg ttatcattcg gtttggtgaa attaatgttc aaagtaagga
    11401 taaaaatcaa aaagatagta gcggaaccaa gtgtttatca gtaagataga atttggaggt
    11461 cagatcatgc ttattgaaga aaaactaaca aaacaagagt tgtttaccac gacagagaaa
    11521 cggattgtcg attacatccg taggaatatc gaagctgcag tctacatgac gattgaagaa
    11581 ttagcaaaag ctacgtatac ttcccattca gctatcattc gtttatgcaa aaaaatggga
    11641 ttcagcggat ttaaagaatt ccgtttcgag ctggggcggg aagttcatca attgattgct
    11701 cagttcaatc aaacgattga tcctaatttt cctttttcaa gtgaagatag cactcagcaa
    11761 attgcaaaga aaatggcgga tttatcgatc cagtcaataa aaaaagcaca attgcagatt
    11821 gaacatgaag atttagatca aatcggtaaa gtattgaaca aggcacagcg aatctttctt
    11881 tttgcaaaag gtgattctca aatcacggca aggaaattcc agaataaaat ggtcaagttg
    11941 aataagtttt tgatcatggc tgaagaatac agtgattctt cttggaatgc agccaatttg
    12001 actagcgagg attgcgccat ttttattagc tacagcggtc gcatccatca ttatgaacga
    12061 ataatgacct atttgaagca tgtagaggcc cctaccttgc tgattaccgg aaatcagcat
    12121 tcagaaatgg ccaaacaggc tgatatgtgt cttgttatat cgcaggagga atatgatttt
    12181 gctaaagtag cgactttctc ctctcaaatt gccttcgatt acgtattaaa tacgttattc
    12241 tcagtgatat attctcaaaa ttttgaagag aatatccgca acttgaaaaa caaacaagaa
    12301 ttgatgcaaa aaggccttct taaagaatat cggaaaactt aagaaaagat cctcatttag
    12361 taataagaaa gatagtgttt gataggctct ctttgatatc atcataaaaa caggctgaag
    12421 ttttcttttt caaacctcag cctgtttgtt tacggaacga ttcgaataga tatattatag
    12481 aaatgaggaa tagctaactt ctgtttctta ttttgaacga ttgacgaaaa agatatataa
    12541 gaaggaggaa aatcagtata catgcagcta acaatccata caggaaaaat cgtttgttct
    12601 cggatacttt ctgtacttct ttttgaactt cttttgcatg ataaggaacc cggtgccctc
    12661 tgacaagtaa gcgatgactg ttgaccatat aaggtgtaca agttaggagt gtcacgaaat
    12721 cctgcccttt ttcgattctc agatcttttg tatcagtcgg ttctaccgtc ttgatctgat
    12781 cgacttgata agcgagtgtc cgtccattga tttcgatata gaaagaatca cctaaagtca
    12841 gtttcggtaa gtctgtgaac aactttgctt gaggcagtcc tcgatggctg gaaataacgg
    12901 catgtgtatt ttctcccccc gtaggataag acgttccttc caataatgag gcaccttttt
    12961 ctagcagcac agtattcgtt tcatcaaaaa ttggcaaatg gacattgatc tcagggatcg
    13021 tcaaaatacc gatcgtatgt ttctcaaaat aggttctatc ctttttcgca ggttcttttt
    13081 tcttagtaaa gggatctgca cctggatttc caccttcttt tgctaattgc tggtttttct
    13141 ttgtcatgtt ttcttggatt ttagccatga cttcttcatt ttctttgacc gcttgttgct
    13201 gataatgaga gatgatctgc tgatccagat aattattcag cgcgtcgcta acaaatggat
    13261 aagctagtat ccctattcct gagagaagca agaaaagcat cagcagatct agaagtttcc
    13321 ttttctttgg tttcatttgt gttctccttg tgatgactgt ttgataataa aagaaaaagg
    13381 agcatttgcg taatccgctg ctcctttttc caattcttgc aaaaagacta aatctgactg
    13441 tgcttgcgtc tagcaaagta cagtccagca atcagtagaa ggactactcc tgcaccgata
    13501 tacacataga ttcccttacc gcctgttgaa ggaagtgtac ctttgtgttt atttggtatt
    13561 ttttcaggag aaatcaactg tcctgctgtt acataagatt gttcatcgat agtaaatgct
    13621 acacggtttg ttaatggaac atatttttct ggcgctttcg tttcttccag atagtacgtg
    13681 ccatatttta gacctgtcac atcgattaaa ccgttacttg tggttgtaaa aaccgttgct
    13741 gattctttcg ccgataccca gctgacggct tttgtggatg ggtcgatcga taaatatttc
    13801 gctgtgtcac tatcttgatc acgaacgacg aattctgctc cagcaagtgt ttggtctgat
    13861 gtaacgtcac catctacttt aacaaatcgt ttgcccccag taacgacatc gactgacggt
    13921 ggtgtttgat catttgtatg cccgttatcg acatttgctt ggttagaaaa tcctttggtt
    13981 ggatctgctt tttcgttcaa atgcatatag taaacgaatt tcaatgtacc gccaggagtt
    14041 aatgaaggaa tataattcgg atcaactgaa accgtgaatc cgtctgtttg ctcagtgaca
    14101 gaataattta ctgggtcgat ttctttattt ccatcatata aggcataagc atacgttcct
    14161 gaagaatcat tatcaaatgt taaagcagcg tcatgagtat cgataagttt gaatgttgtg
    14221 tacttgtttt gcgttccttc tttatcagcg atacctaatg ggatattgac agaaatctca
    14281 tattgaattt tttcaccgat tgcgacatct tttccattca attgaggtgt tgttttatca
    14341 actttagacg tatcattttt gatggtcttt tctactggtg tttggctatt tgccaggatc
    14401 tcaaaaggcg tgattgtttg cttttcaatc atttccgcat tatcaggagc ttttacttct
    14461 tctaaattat attttccaac ttctaaatga ttaacgatca actggccttt ttcaatagat
    14521 gcctcggcaa agtcattgtt gccgatgtca taagaatgac cagtaatgaa atgtttggct
    14581 ttggtttgat cagttgtcca agtgtacaat ccatctgtga cactttggat gtattttttg
    14641 acgcttggtg ttccttcttc tttagaaata ataaattctg ctccatttag tgcttcgttt
    14701 tcggcagtac cgatttttgt aactttcaac gttccatcat taccgactgt atttttaggg
    14761 tagagatgga tagtatctag ttcttctgtc ccgtatttat aagagccatc tgcttgtttg
    14821 atcatctcat atacagggaa agctaaaacc atgtttgcgg cagctgacac tccgtctttt
    14881 ggttcttctt tgatcgtgta gactgcatct ttcccatttt gttttttagg taaagataaa
    14941 gtgacatttc catcagcatc tgtcgttcct gaagcaactg gtgtaccagg agtcaaagac
    15001 tggactgctt gttttgctgc atccacggac gctcctttat ctcgttgcgc ataaaattct
    15061 tgagtgacgt tataaactga aaatgaaata tcggctaatc cttggtattg atcgaattcg
    15121 ctcatttctt tcccgctgtt ttggattaaa ggatcgggta aatcagtcat tttcttttta
    15181 tgaaggatga cttgagctgc tgtctcctct gcaaacactt tttttgcgcc gaagctgttt
    15241 gtgagtaatg gtaaaataag gaaaaggact cctaacataa cgtttatttt tttatgattt
    15301 ttcatttttt cactcctgtt ctttttttga taaacaacca gattgcaaag cttgcgaata
    15361 ccataccaac aacgcctaaa agtgtatttt tcgtttcgtt tgtttttgga agaaatggac
    15421 ggctattttc ttttttattg actactttca atatactgtc cgcctcttta tcctcagaaa
    15481 gaaaagttga aatggtaaac ggaatttctg tttcacttaa aatataacca gaaggtgctt
    15541 cgatctcttt taatcgataa cttcctgttt tcaacccgga aattgaaaaa gcgccatttt
    15601 tatcggaaat caggtgaagc gcatctgatt cgttctttgt ccacttgtat ccgttggctg
    15661 tttgctggag gtaatttcct gctttgtttt taataaggaa tactgcgcca gctaatcgtt
    15721 tatctggttt atttgtctcg attttgacaa attgcttact ccctgtatat acagagactt
    15781 tctttgtact gacaccaaaa ccaaaatcca gtgttccgtt gttgttgatt tccttgttcg
    15841 cctgtgctgt actgcttagc tgcatctgat aagaaatggt cagttttttt ccagcatatt
    15901 tttccaacga agggatcgaa aataaaacat caaatccgtg aggtttcttg tgtatgacaa
    15961 agccttctgt cagcttttct ccattcgatg aaatcgttaa actattaggt aaaaacgtta
    16021 atgcttcatc cgcactatct gacaattcga acttttgata gtcaaggata tttacaggaa
    16081 ttgtcgtatg caaggaataa gtgatttttt caccaatcgt aaaatcattt ctaggctctt
    16141 ctaatacctt ctcaaaaggt ggatcagggt agtcgttttc ttcattttta ggatacaaat
    16201 ggatagttga aagtttttga ttgttttgtc catgtacagg aagaacaact accatattct
    16261 ctgccttttc tttgacgact tctggtactt tggattcaat gaaaagatac gctttatctc
    16321 ttttttcttg atctttagcg gccagagaaa aagaagcgat accatcttcg ttgttaagag
    16381 ttgtagttgt tgcttctgcg gtaaacatac cggaagacgc accgtttttt gcgatctctg
    16441 cttgtgcttc ttctaccgtt ttgccctttt cccgtagatg gtaaaaagaa tctgtgacat
    16501 cataaacttg gaatgtgaca ccatttaatc ctcgatacgt ttgtaataaa gctttttctt
    16561 gtccgtcatt tggatgattt ttcggcagtt gcccgtttgg gaaaagcaat ttgtgtaaag
    16621 taatctgaac catttctgtt tctgtttctg ttgctacctg agtaaaagct ggtttaaata
    16681 gtaacaagaa gagacacata ctaagccaac caagtttttt catatttagc tgccccctct
    16741 ttcttttttc aaaaaaagat accaaataga aaacgcacaa ccaatcatcc ctactagata
    16801 gattcctaaa cggccaattc ctcccgtttc aggtaatggt acttttgcct gattcgtgat
    16861 gtctaaagaa atctggttgt ttttggctcc tggtgacaga acagattcat gatcttgtcc
    16921 atccacttga attgacccat cttcgtgtat aactatagta actggctctt ttagaccttg
    16981 gtatccttct ggtgtaaaag tttcggtcag cgtataagtt ccaggtttta aattttcgaa
    17041 tagaaaggta tctgtttctt gtccatcttt tggcgattct aagtccattt ctggtccctg
    17101 cagtctgaat tttgctcctt ttaatgtctg tccgttgtct tcttttttgt gtaccgttag
    17161 atcaaacgct tttagtcgat tttgatgagt gagcgtccat acattatttg tcatctcggt
    17221 attttttccc gtgtaaatga tttttccata tttatcgatt tggaattcat aatttcccgg
    17281 cgaagtatca tatccggcag gaccagctgt ttcaacgact cgatatttac caggtgtgag
    17341 tgaatcaaaa ctggcaagac ctttttcatt cgttgtttgg ctgtcaattg gctgataagt
    17401 accatttgct tcttttttct gtagggaaaa tgctgctcct ttaagattta tctctttgtc
    17461 cgtcccttgc atcgcatact tacgtactgc tacaggaact tcaacaaaag gattttcaat
    17521 agtcaactgg atcgtatcat cggaagtagt gactgttttt ccatcaatgg ttaccgtacc
    17581 atcagaagcg atcttgattt cccaagtagt ctttttgctt aatccatgcc cttctggagc
    17641 ttttgtttct gtcagcgtat aggtcatttc tttttgcaat ttgacatttt ctggaagaga
    17701 ataggttccg tcgccatgat ctgttagtaa agtatcaata gaatctcctg ttaatttgaa
    17761 aacagcgcca ataagatcct tttcacctgt agaggaattt ttcgttattt tcaagtttaa
    17821 cggtgtgaat tgtttagtat ttttccatgt cattgcatct atttgttgag aatcgtatcc
    17881 tggtacaggt aattctttga ttgtttggta actgaatgct tgaccttgat tattgtattg
    17941 aggtagtgat aatatctctt gataactttc tccgctattt gcagataatt tgtcaatgtc
    18001 tgcacgttcc cacgtatttg ttgtatcttt agctggttta atgattcgaa tatatccgtt
    18061 tttccaagct gcagcatttg tcgtatgttc ccgttgaatc tcaaaagtaa cttgatctgg
    18121 acgatcagct agattgttgt caaattcttc ccataacttt ttgatgtgaa gactgactcc
    18181 gggagcttta gcagatggta taccgaattc agctaattca ttggtatcaa tgtttggctg
    18241 gaaagttgtc ctgccgttca tttgatacca gaaatttgga tggaagtcct cattttctgt
    18301 ttggattctc acttggtaat ggatttggat ttcttggtct tttcctaaat agatctgatt
    18361 gctcttgatg gtatttcctt ctatggaaat agatggagtg accgttgtag gacttgtccc
    18421 cacactcttg actgaaagtg taccaggctg ataaatgaat ggttctgcga ttggatcatt
    18481 tatttgtcca ttgcttacag ttgctgagat ctgtacagct tttttggcta ggtattcaga
    18541 gatatctgtt gcatgatcag ctgattcata gtaaagatcg cctttttcat ctgatgaaac
    18601 catttgtcgc atacgagact ctacttctgc ttttgagaga ccagctgccg gatcgctttg
    18661 aagttggatg ccaagaccat gtatttcgat tcctcgttct ttgagtgcca tcgcttctcc
    18721 gatggttgcg ataaacgtac tgtcgattct tctggataga ttgttttggt caggtgcatc
    18781 atagattctt gagattagag aagtatttcc cggccgatct tgcgtattag aaaactgagt
    18841 tccgtaataa ttgctgcttg attgtgcgtg tacccgctgt actttatagg aaaatgttgg
    18901 tacaccatcc gtcagcaaaa cgatcacttt tttatgtcca tttggaacgg atagcatgct
    18961 tcctgcatct cttagtgctt tttgtgtaaa agtaccacca tttgtccgtg aaggtgtaat
    19021 ggattttact tgatttttca ctgaatcaaa tgaacccatc tgtactgcac cgttactgta
    19081 gctatatcct tcgcttgagt agccgacata tcccatattg attttgtctg tgataccgct
    19141 atctgctaaa gtatcgacaa aacgatcgac accaatcttt acttcaccga tccgattatt
    19201 gtcgttcata cttcctgacc agtctacgac caatacgaga tcaagaggcg tgatatcttt
    19261 ttgtacattt ccacgagcat tcaaataaac atcaaacaat ccgggtgtac ttgtttcctt
    19321 tgcatacttt cgtatcgcat agtctgcttg attaccggtt cctccatatt cgatatacga
    19381 atgggtccga tcattaggat ctccattcca agaatttata ccatcccatt gatttgttcc
    19441 gtttttattg ccttgatgat caagaacatt tgtatttcct gtaggttgcc aactggcttt
    19501 aggataagtg ccctgatcat ctgttgtata ttctgtagga atcgtttgat tagatggcac
    19561 actcacagct ggcaaggata atgtaggcaa tgtaaatggg ctgacagaat tttcgttttc
    19621 ttttgatgat tcttcttcta tttcgtctgg ctttggtact gacacatctg tatctttctt
    19681 attttctgtc tcatcaataa tgtcatattc tttattatcg acgatcagct tgtttgcctg
    19741 gctgtggtaa agtgctggat cgattggaaa actcagatcg atagtttgtt tagtgtttga
    19801 tgagatttcc aacgtaattt tcccgctatt tacctgataa gcatagccat ctccttcttt
    19861 tctttccaca tcacttaagt aattttcgac tagaccaatt tctttttcta tcttttcagc
    19921 actggtgtta ttcgcattta gcttgatgtt cagtgctttt tttccagact gatccgaaat
    19981 gacttcagct gaaattgttt ctaaaactgt tggatcatct gtagtgtctg ctattgcaga
    20041 taatggcaca aagtttgata aactcaatat tatcagcatg aaaataactt tcagtttctt
    20101 ccctgtggtc gtcatttttc ccctcctttt tctttttttc tgttttgacg tgaatagtga
    20161 aaattattaa aataaacagg cgttacataa ttttatttat tttaatatga taattattat
    20221 acgaagggcg ctaaagggaa tcacaccaat tttcatacca attggtttca tttatgaagt
    20281 tgattccttc gattaatgag cgtttcattg aaagaaaagg gttgagggag aaaaacatat
    20341 gatcaggttg ttggaaggaa actatagaaa gatggaaaaa atcattcgaa ttctgtccat
    20401 ttctgacaag tggatgacta aaaaagaact tgccaaacga attggtagtt cagaatctac
    20461 atttgtaagg tatatcgaag agatcaaaat ccgctgggga acggatgtaa cgattcaatc
    20521 ttcacaaaaa ttggggtatc gtcttgaaca tttgaatgta ccagcttatt taaatgttct
    20581 gatggaaatg tcgcaaagtt ctacaaacag tcgtttatta aatgagatta ttgtcaatcc
    20641 gggtaaaacg attgagtatt attgtgaaaa aatttccatc agccggtctt cttttacacg
    20701 taagttgaaa caatgcaatc aaattttaga aagctattta ttaaaagtga tcgttgacca
    20761 aggttttcag ttaactagcc ttaaaagtga gctccagttg cgtatctttg ttaccttttt
    20821 ctttttaagt tactacggaa gacgcacgtt gccatttcca atcgaaaaaa agaagatcaa
    20881 agaaattatg gtcaagaata attgtcagat gaatttgttt caggaagaaa gcccatatga
    20941 gcaatctttc tttattatgt attttgttgt gcatctcatg agagaatccc aaggaactcc
    21001 atttgaatgg aaagctggag actcttatgt acagttgata aatatcgata aaaaagactt
    21061 cttaatcttg aaaaatctct taggaaaatt gaagtacagc agctatataa aagtagtgaa
    21121 ctattttgtc aatacgattt tttcagaatt ttcggagaaa gaaaaaaatg aaatcagtga
    21181 gcagatacga aaaaatatgt cctactgttc gttttcatcc gctgattgca aagaaaaaaa
    21241 cgaagaatat gttgttcaat tattaacgca aatttatttt ttctcccgta ttttcccata
    21301 cgatacgact catttgacga tccgtgcaac ttactttgca cagcagatgc aacaccgcca
    21361 gatacgtcta tatgctggat ttcgtgcaga acttcaattt tattccactc tactgaatca
    21421 gaatctgatt tattatttat ctagctatat cttttggatg cttatcggta atcctgaaat
    21481 tcaccattat acttctgata aaaaaatact gattatcagc gacttaagtc ttcgacatag
    21541 tcaatatatt gaagaatata tcagtgatat tcttgctgtg cataaaattc actctgagac
    21601 aactgctatt actgaagatc agctgtcgaa atacaatctt ctcgagtatg atctgatcgt
    21661 tacgaatcaa cccattttga atgcccatgt tcctcatata ttgattgatg atagtgtgtc
    21721 attcgcaaat gaagaagaac tcatccgctt attcgagtta tgaataatca aaaaacagct
    21781 atgtataaac caccctgata aaaatttcag gacaaataca catagctgtt tctttttatt
    21841 gtattccttc agcaatccaa cgagatttat agcgaaagtc ttttaacaac tcttcttttg
    21901 tccaattatt tgcaatgaga tccgtcaaag tgttcattac gagggtcatg gaaaagtata
    21961 ggaaactaac tgcttcttcc gacgattgaa tggagaaatg attgattctt aataattcca
    22021 gccatttttc gttcattgga gaatcagccg atagttctct acgtttggtt gtctgctgat
    22081 catttcgctg ggttaacaag tataaccttt gccaatagat actttcgtga tctaagtcgc
    22141 agcaccactc taaatatttt tcgatcccca aaaagaaatg ttgtttgttt tcgctaatga
    22201 attttaagat atcttgatgg actatgtgag aactgctttt gatcgtatac aaataagcat
    22261 cttccaaatc ttgaaaatat ttataaaaag caccgcgcga catccccatc ttgtcaacga
    22321 tttctgagac tttgacttgg ctgatgtgct gtgagcaaaa tatctccagt aataggtcat
    22381 cgattctctg tttctttgcg ttcgataaat tgaaaaaagt ttcttttggc atccacatct
    22441 ctcctttatc tctgattata cgtggatagc ccttcattgt catgaaggat tcattccttc
    22501 tttttctctt cttctgtcaa gtacccatct tccattcgat aaacgcgatc actccattga
    22561 atcatacgga catcatgagt aaccataata gtcgcttttt gtttctcatg agcttcttta
    22621 actagtaatt tgacgacttc atatgcatga tctgtatcca aactagctgt tggttcgtct
    22681 gctaagatca agcttggctc attatataaa gcgcgagcaa ttgccactct ctgtctttct
    22741 ccaccagaaa ggtctttagg ataactattt ttaagtgact ggatatctaa agattgcagc
    22801 aaggaatcga tttttgttga atcttctttt tttgaagtaa cttggtcaac tagttcaaat
    22861 tgttccttta ccgttaaaaa aggaatcaaa ttagagcttt ggagaataaa tcctatttct
    22921 ttaaagcgta aatctgctcg ttttttttct ggaagctcag ttagtgagtg accgttgatc
    22981 actatttctc cggaagtcgg tgtttgcaat cctcctgaaa tggtaaggaa agtactttta
    23041 cctgaaccag aaggaccaat gatgctgacg aattctcctt cctttacata aaaatcaatt
    23101 gctttcaaag ctgagacctt tgtatgacca gaaccaaatg ttttggtaac ttttttcatc
    23161 gttaatattt ttttcatttt tattaacctc ctattgctgt aattgggtct actttggtta
    23221 tcgtacgtat cgaaaacaat ccgcctagca ttgcgacaat gatcaaaatc ccactatata
    23281 gcaaccattg tgaccaaaaa acagcaaaag gcatcgcgct tggtaaaatc agactagtca
    23341 aataagcaaa caaaagagca agcagaacgc ctaccacccc aacaataaag gactgtgcta
    23401 ctaaagattt agcgatgaag aaatttttga tcccttgtgc tttcattact ccaaaaatag
    23461 cagtcttctg aagtgtcatc acgtacatga aaataccgac cacggcagca accactaaga
    23521 acaaaaagta tatcatcgca tttaaggtca tattttgggc agaatagcca ggaatacttt
    23581 caatgaatgt cccaatagtt aataactgta gacccttcga tgctttttct ttcgaaattt
    23641 tagctgaatc ctttattacg atcccattga tcggctgttc ttcttcagaa gcaaacggct
    23701 gttgtccata cttcaaagca gtccacgtat ctagggacgc atagatgacg ggagaaactg
    23761 tgtaataagt ttcaggaaag ataccgacaa tcgtcaaagg atcgtcgtaa ctgccaattt
    23821 tgatcgtatc tccaatttta tagcctgaat cagccagatt ttgagagatg atgatttcat
    23881 tctttgtttt aaattcatgc cctttcgtga gttttggtaa taaaaatgct tctttcgttg
    23941 tgccgaaaac tgtaacattt tctttgtttt ttcctttgat tgctccgctg tacaatccga
    24001 ttggtgcttt ctctcctcct tcaatgtatt tcagatctcc tcttgttaat tgtgaagctg
    24061 caaatacttg atttgcatcc tccgacaaaa caatctcttg tgcgtcccaa tcatcgattg
    24121 cttttttaaa ctcttctgaa agtccattcg ctagtcctga caacatgaat acgacatacg
    24181 caatcaagaa cataataccg acgatcaagc catatcgtaa tttagaatag cgaatttctt
    24241 tgatagctag aaacatttgt ttttcctccc tatgatagat tgatagtgac aaaatgtcac
    24301 ttgttgaata gtgtaactct aaagtgacaa tttgtcactt gtgttgtttt tttcttatca
    24361 ataaattgtc taagacatct ttcgataaaa ccttaacatg agtcataaaa aatttattta
    24421 agccttattt atgctttcca tgataaacta aagtgaccaa atcaatgagg tgggaaaatg
    24481 attaaactat tagtagtaga agacgataaa gtattatcca ataatataaa agaaatactc
    24541 gctgcaatcg gcgagataac acaagtttat gatggtagtg aaggactgtt cgagttggaa
    24601 agtggagttt atgatcttgc aatcttggat ttgatgatgc cggaaatgaa tggatatgaa
    24661 gtgttagcaa aaatacggaa agaaaaaata cagactccag tattgatttt gactgcaaaa
    24721 gatagtttgg aagataagat tgaaggattt gaacgaggtg cagatgatta tctaacaaaa
    24781 cctttctatc gagaagaact gatcatgcgc gtgaaagcat tattgaaacg ctcgctaggt
    24841 ctctttgatg aaaatgttct gaagtataag gatatcagtt gtgatttaaa aagcaatgaa
    24901 gtcttgtata aagaacaagt tttgcccata caaggaaaag agtttgaatt gctcgtttat
    24961 tttttacaaa ataagggaat cattttgaca aaagagcaga tatttgaccg gatctgggga
    25021 tttgattctg atacgacgat tacagttatt gaagtttata tgagcaactt acgaaaacat
    25081 ctgcgaagta ctggattgga taaagaaatc aaaaccttac gtaatgtcgg ttatatactg
    25141 caaagggtgt gatgcaatga atggagatat tacacgaaag caacgtagtc gcttttttgc
    25201 gacaaatgta gcagcatttg cttttatctt ttttcttttg ggattcatta ctttacagat
    25261 attaaaccaa tctgcataca gagaaacaga tctgtcatta gagaaaataa attttgattc
    25321 tcgaacagta caattagaga tacaaagata tcagcaaggc gatccctttt taaacccgac
    25381 tcaaacggaa caaaacagaa caaacatgga acagccatta atggaaccat caaatgaccg
    25441 ttttaacact caagtgatct tgtggtctaa aaatggtgag atattgaata aagcagctat
    25501 tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc tgaataccat
    25561 ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc ctctctttcc gttcgattac
    25621 gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc caaattttag aaaatacaaa
    25681 tcaaatcaag aattcattag cgacttttcg cacgattatg attttgtgta tgattgtttt
    25741 ttggattctc tctattggta tcagttatta cttgtctagt ctaagcatgc gccctattat
    25801 tctttcttgg agaagacaaa aagaatttgt tgagaatgct tctcatgaat tgcgcacacc
    25861 gttaacgatt attcaaaata gtttggaaca tctttttacc aagcctaatc atactattat
    25921 tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca ctggattgac
    25981 ttcggatttg ctgacaatcg ctcggaatga ttctgatcag cagctgcttt caaagcaaat
    26041 gatcaataca caagaatata tcgaacaact tgttaaacca tttcaagaga tggccatcat
    26101 agatggaaaa gagtttattc tccataacga tgcaacatta gaagtaccaa ttgatgaaaa
    26161 aaaaatccat caagttttgg ttattttgct ggataatgca ttaaaatata cgaatccaga
    26221 agataagatc attttggatt catctgttaa ccataaagaa tggctgattc acgtaaagaa
    26281 tactggccct tctatcagtg aagaagatca agcaaggatt tttgatcgat tttatcggga
    26341 agatcaatct cgggcgaaag agactggcgg atacgggctt ggcttggcaa ttgcaaaaca
    26401 ggtaatcgaa cagcatcatg gacagatttc tgtatccaat tatcttccac aaggagtcga
    26461 ttttaaagtc caattgccgt tgaagcctaa aaaataatgc ttatccctaa aaaagggctg
    26521 tgacaaaaat cttgtcacag cccttttttg ttgcgaataa acggtgttcc agaagtagct
    26581 tcttcggaaa taagccgaaa tttctttaag ctgaccactt ctgtcacaac ctctttagct
    26641 tacgcttacg attttttatt gcggtcttca atgtgtacat caccgcttgt gtcaatatcc
    26701 aattcttctc ggctaacatc ttctgtcact ttttcaacat cttcatgttc ttctttatga
    26761 atcccaactt cttcacgaat aactgtatgt ttgcttacat ctacttgctc ttctttgatt
    26821 gggatagtaa tcgtgtcatc ttccaatttt tcgtctgttc cctgtgaaga ttgatctgtt
    26881 actggtttgc gttcgatgac gatttcttca cgtttgaccg gtacttctac cgtttcagta
    26941 tcgttgacca catgtttgtg gatgtcgact tctccagttg tcacatcatg tgtatttaca
    27001 tctaattttt cttcttttag ttgaattgta tcatcatcta gatttggttt gcgatcgatg
    27061 tcttcagtct tgcttgtacc cattgtatca gtctcgccag gtacgtccat cggatcagtt
    27121 cctggtgtca tattcaatgg atccgttgta ttttgaaagc ctgtcgtgcc acctacgtcc
    27181 attggatctg ttccagtgcc agtttctaat ccagtcccca ttgtgcttgc tgggctaaca
    27241 gtatcttgtg tatccattgt atcttctgtt gtttctggct gacgatagcc ttttactgtg
    27301 atgacaaatt ttccatcaga aatatctcct ctatattgat ataaaggatc attttctggt
    27361 gtagccgtct cagaatcata ggtatcaaaa gaaaaagtat cttttatttt ttcccataaa
    27421 gaacggtctt cttcaccatc catccgattt tcatctgttt caacatctat accagacaat
    27481 ccgtcaaatc ctagtctaga tgctgcttca tcagtagtat aaataacgat atcatctcgt
    27541 tcataccctt cgttacgcaa ccgttcaatt actgataatg cttcttccct cgtactatag
    27601 cttccgatga ccgttaaatt tttctcttcc atgtaaattc ctcctagcaa atagatttat
    27661 gattggtata ttgttactta taaatttatt ctataggaga cgattgtttt ctacaaatat
    27721 ttagattgga atttgattaa aacaatttaa ttgaaatttg aaaacattta tgttaatgaa
    27781 agtcatattc taataaaatg tcatttgttt ctatatgaat ttttatgaaa aatttca
//
LOCUS       DAZKBH010000025        25624 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000025 DAZKBH010000000
VERSION     DAZKBH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.025"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..827
                     /locus_tag="ACG8OO_001074"
     CDS             273..827
                     /locus_tag="ACG8OO_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9044970.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1000..1437)
                     /locus_tag="ACG8OO_001075"
     CDS             complement(1000..1437)
                     /locus_tag="ACG8OO_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9044971.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1485..2432
                     /locus_tag="ACG8OO_001076"
                     /pseudo
     CDS             1485..2432
                     /locus_tag="ACG8OO_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2703..3138
                     /locus_tag="ACG8OO_001077"
                     /pseudo
     CDS             2703..3138
                     /locus_tag="ACG8OO_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3154..3987
                     /locus_tag="ACG8OO_001078"
     CDS             3154..3987
                     /locus_tag="ACG8OO_001078"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9044972.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4232..5257
                     /locus_tag="ACG8OO_001079"
     CDS             4232..5257
                     /locus_tag="ACG8OO_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9044973.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5395..5520
                     /locus_tag="ACG8OO_001080"
     CDS             5395..5520
                     /locus_tag="ACG8OO_001080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044974.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5545..6660
                     /locus_tag="ACG8OO_001081"
                     /pseudo
     CDS             5545..6660
                     /locus_tag="ACG8OO_001081"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6743..7639
                     /locus_tag="ACG8OO_001082"
                     /pseudo
     CDS             6743..7639
                     /locus_tag="ACG8OO_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7655..8479
                     /locus_tag="ACG8OO_001083"
     CDS             7655..8479
                     /locus_tag="ACG8OO_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9044975.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8511..9821
                     /locus_tag="ACG8OO_001084"
     CDS             8511..9821
                     /locus_tag="ACG8OO_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFP9044976.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9969..11228
                     /locus_tag="ACG8OO_001085"
     CDS             9969..11228
                     /locus_tag="ACG8OO_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9044977.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11238..12110
                     /locus_tag="ACG8OO_001086"
     CDS             11238..12110
                     /locus_tag="ACG8OO_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9044978.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12122..12952
                     /locus_tag="ACG8OO_001087"
     CDS             12122..12952
                     /locus_tag="ACG8OO_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9044979.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12992..15175
                     /locus_tag="ACG8OO_001088"
     CDS             12992..15175
                     /locus_tag="ACG8OO_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9044980.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15236..16456
                     /locus_tag="ACG8OO_001089"
     CDS             15236..16456
                     /locus_tag="ACG8OO_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP9044981.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16453..17913
                     /gene="gtfA"
                     /locus_tag="ACG8OO_001090"
     CDS             16453..17913
                     /gene="gtfA"
                     /locus_tag="ACG8OO_001090"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFP9044982.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17990..19096
                     /locus_tag="ACG8OO_001091"
                     /pseudo
     CDS             17990..19096
                     /locus_tag="ACG8OO_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19418..19627
                     /locus_tag="ACG8OO_001092"
     CDS             19418..19627
                     /locus_tag="ACG8OO_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9044983.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19620..19769
                     /locus_tag="ACG8OO_001093"
     CDS             19620..19769
                     /locus_tag="ACG8OO_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP9044984.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19923..20588
                     /locus_tag="ACG8OO_001094"
     CDS             19923..20588
                     /locus_tag="ACG8OO_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9044985.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20872..21957)
                     /gene="xerS"
                     /locus_tag="ACG8OO_001095"
     CDS             complement(20872..21957)
                     /gene="xerS"
                     /locus_tag="ACG8OO_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFP9044986.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22291..22524
                     /locus_tag="ACG8OO_001096"
     CDS             22291..22524
                     /locus_tag="ACG8OO_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFP9044987.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22521..22928
                     /locus_tag="ACG8OO_001097"
     CDS             22521..22928
                     /locus_tag="ACG8OO_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP9044988.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24065..24583)
                     /locus_tag="ACG8OO_001098"
     CDS             complement(24065..24583)
                     /locus_tag="ACG8OO_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFP9044989.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            24950..25198
                     /locus_tag="ACG8OO_001099"
     CDS             24950..25198
                     /locus_tag="ACG8OO_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044990.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         8727 a         3912 c         4773 g         8212 t
ORIGIN      
        1 ataatttaat tatgtaaatt agctattcta agaacttcta cggataactg cccgtctaaa
       61 atttaaataa gagcttagaa tcttcatata agtaaggtta atttttttgt acaaaaaagt
      121 cggaatattc tgagttactg aaatgatata aaatcataaa ctaaatccct tgattttatt
      181 gactttcaaa agtttatata tataagttaa tgattatggt aaaatatata cataatcatt
      241 aactcagaat atttttaaga agggaaacaa tagtgagcta taatgtacaa ccgttaagga
      301 caaatgaaga gatacaagat tttcagtttt ggctaagaag aacaagtaat ccggaaagag
      361 atagtttttt atttttgttt ggaatcaata atgggttacg tatgtcagat attataggat
      421 taaaagttgg agatatacga ggcaaaagta agcctattat tgttgaacat aaaacaggta
      481 agcgaaaacc gatttttata gataatttga gagaagaaat tttattatat actgaaggta
      541 aagaagaaaa tgattggtta tttccgagtc ggcaacaagg tcgacatata acaagggatc
      601 gtgtctatca gatatatgca gatatagcag aaaaattagg acgagatgat ataggaacac
      661 atacattaag aaaaactttt gggtatcact actacaagaa aactagagac attgctacct
      721 taatgtttat ttttaatcat agctcgcaag caattacaaa aaggtacatt ggtataactg
      781 aagatgaaat aggagcatca cttcgtggat ttaaactagg tgtataattt aaaaattttt
      841 ataaacagta aagacaaaga ttaaaagcta tttttatcta gaacgatttt atttcataaa
      901 gttcgcggaa taaaagctgg ggttactggt acttggtata ggttcgtgtt atataaacta
      961 agtaattaaa taacattttt aggttggaaa aggatgaaat taaaaaaaga gaatcaatac
     1021 ggtctcaaaa acgcaaacgc tgcggaataa gaaaaatact gataactcaa cgtttagcag
     1081 actatctaat agggatagtc tgcttttttc gtttttgacc atacttttga ccttctgcaa
     1141 acttccttgc ttttcctctg tagaactcat gaaatctgca aaacgaattg ccgtttcctc
     1201 tgtctgcttt ttcgcgatat gggtgtatat atcatccata gtggttttaa tatctgtatg
     1261 tccaagtcga acatacactt ctttgacaga cgcaccagat tcaaagagca gactacaatg
     1321 ggtatgacga aaaccatgaa gtgtgatatg ttttaagtta ttctctttaa tgatgatttt
     1381 caaattgtga tttacatagt ctaaataaag gggcttattt tcttctgatg aaaacaaaag
     1441 ttgttcacca tcttttatag gtttgattct catttttaga tattatgccc gcacgtattc
     1501 atcacatttc tattgtgaat cgctttattc gaccgacatt tgacttttat cataatatac
     1561 tcggtttaaa attactaatg aaaacaatta atcaagacga tcataccatg tatcacctgt
     1621 ttttttcaga taatcaccac cgtgtgggaa ctgaactgac tttttttgaa gtacaagaag
     1681 ggaacaacca gttttttgga accaatacga ttgaacgaac gatactaaaa gttccttccg
     1741 aagcatcatt gcatttttgg gaaatatatt ttgaaacaca aggcgtttgc cattatggaa
     1801 tcgaatcatt ttctggacga cctatcttac gttttgaagg gccagatgca acacgattag
     1861 ctttagttcc cttacgcgaa tttgaagata ttgatgatta tttcccgtat gtcacagacc
     1921 agatccctac agagcatgcc attttaggaa ttgatgccat tcaactacgc gtacaatatt
     1981 ccgatgcgac tgaacgagag ttattgtcgg taggctggta gcacaaagaa tacgtcagtt
     2041 tcttttcggg tcatcaacga gtgacaatct tagaaaatca agacactcag ttctatcaag
     2101 aagttcagat tattcaagat cgtgagaatc cagttgccat tgaagggatt ggtagcgtcc
     2161 atcatgttgc gtttggcgta gaaaataaat ctgatttaca acgcatcgac aaacaattac
     2221 aagagcgcaa ttttataaat tcaggaataa aagatcgtga attttttgta tcactttatt
     2281 atcgtgaccc aaatcaatta ttgattgaaa ttgctacagg ggaaggaaac ctagatgcaa
     2341 aagcgtatga aaatcaaagt ccacgctttg aagaaattcc tttattttta ccgcagtact
     2401 taaatttcat tcgtgaacaa gtggagcaat aattagctaa tcaaatccat gacgaagagt
     2461 aagaggttga ctcataagtc aaaaaataaa gaaaaacgca tcaaatccat tctaaagaat
     2521 gttgatttga tgcggttcct atttttataa actatcattt tcgactaata aaactactta
     2581 tgagtcagcc tctctttttt tcttgatttt tatacctctt tctaggtaaa ctaaacatct
     2641 gccaaaaata ccgtgtctca ttgaaacatt ctcaactatt tcaaaataaa ggggagtacc
     2701 aaatgaacga gtatattgaa attatccacg caagagaaac aacttaaaaa tgtcagtctc
     2761 aaaattccca aaaataaaat tagtattttt accagcgtat ctggttcagg aaaatcatcg
     2821 attgtttttg atactattac ccaagaagct ggtcgtcaac tgaatgacac ctatgatagt
     2881 tttacccgtt tatttttacc taaatacagt cgtccagagg ttgatgagat tgataatctt
     2941 tctacggcga ttgtggtcaa tcaaaagaag attggtggca gtgcgcgctc aacgttaggg
     3001 acgattacta acatcaatgc gttatttcgt gtgttgtttt catgttttgc aacacctagt
     3061 ttaggatatg caaatgctta ttcatttaat gatcctagtg gaatgtgttc tgtctgtcag
     3121 ggaatcggta agacgattgc atttttaata ggattgcttt atttttcggc tattgataat
     3181 gctgattttg tattttctga tccatttacc gcagaagttt tgtctggaat tgaggaacag
     3241 gcaaagaatc atggttattt tgtattggtg cataatgtta attcagcaga agatgttaaa
     3301 actattcaga gaaattggcg ttttgagggg ttcattgttg ttggtgtacg tgtaaaagaa
     3361 tttttggaat taaataacgt ttttgatggt cctgttgttt atattgatac gcatctttca
     3421 aaagagacat taagagaact tgaacaacaa atgaatcgtt tatttgtaaa tacaaatgat
     3481 taccaagcta gtacattggc agtggaacat ttgattcatt atggtcatga gaaaatagcg
     3541 tttctttctt ttgactacat tgtaggtgaa ccaagtgtta ttcaagaacg acatcaagct
     3601 tatatcgata cattaaagaa atatcattta acctttaaat cgcattggat atataacaac
     3661 tctgaatttg agcgaattta tcaagaatta gctgaattta ctgcaattgt tgttactgga
     3721 gattatttag cggctaaatt agtaaaattt ttaaaaaata agaataaacg agtgatggaa
     3781 caattatcga ttattggatt tgatgatatt acttttgcag aattaatgga tcctgcgttg
     3841 acaacaatac gactaaatcc aggtaacaag ggggcgattg cttttcacca actgattgga
     3901 ctggccaaac aagaagaaat aaaaaatcaa gtactactat tagagggaga attgatttct
     3961 cgagactctg ttcacaaaat caaataaaaa agctctgtga aataagtata gttaacaata
     4021 aaatatccga acgatagaga gaatgaactg tgatctagca gttcctcgtc gcaaattcac
     4081 tagaattagc attatttact ctaaattgtt cggatatttt ttcttgtttt taattatgat
     4141 tacagtgcct atttttaacg aaatttgctt attttcaaaa tgtatgatta tatgttagta
     4201 aacactttca aatatgaaaa gaggagctat tatggtagga attagggatg ttgcaaaacg
     4261 ggcgggtgtg tccattgcaa cagtatcgcg cgtgctaaat gaggatccga cactttcagt
     4321 aaccgatgaa accaaacgaa agattgcaga atcggttaat ttttatcatt atgtcaaaaa
     4381 aagtcccgtt gcaaaaaatt taacaaatat tgcattaata acgacagttt ctgaagtaga
     4441 tgagttggaa gatccctatt ttcgaacgat tcgcagaggg attcaagtcg aagcagaaca
     4501 aagcaaagtc aatttgaaga aaattattcg tttgtcagaa acaccgttgg agcaagacga
     4561 tttgctaaaa tgtcaaggtg tgttgattat tgggcaggtg ctatcttctg tgattgaaga
     4621 agtatcagca attaattcga atgtcgtagt gattgatgac cctagtattg aagctgaagt
     4681 agatgcagtt tatactgatt taacaaaagc aacgaatcag catttagatc gattgtataa
     4741 aaaaggacac cgaaacattg cgtttatcgg aggaggacgt gttcgtttag atagccaagg
     4801 aaataaacac gtgtcagatg atgattatcg aaaaatagcc tatgagacgt ggatgaaaca
     4861 aaaaggatta gaaaaatata gtcaaattta tttagaaggc tggaccacac ttgacggaat
     4921 gcaggcagca gatttacttt tgaaaaaaca tgctaaagat aagttgccga ctgctatcat
     4981 ggtaggaaat gatcctattg ctgtgggggt ttatcgttca cttcaaaaaa atggtctaca
     5041 tattccagaa gatatttcga ttgtgagttt tgataatatt gaagttgctg aatttttaac
     5101 acctgcatta aatacggtga atgtgaatac agaagaaatt ggaaaactag cagttcgcat
     5161 ggttaatgaa cgaataaaag aagttagaaa tgtttcgatc aaagtgattg tttctaataa
     5221 tattattgtt agagaaagtg aaaaaaatat cgattaaaaa agtaaacatc caaaaaaggg
     5281 tgtttacttt tttgtttact aatattttta gtaaaaaatc tcgacaaaac attaagtaaa
     5341 cagttatggt gttgttaaca aaaagaaaac gcattcaaaa caggaggaat taagatgaaa
     5401 catttcaaaa aaatagcttt aacagggata gctgttttaa ctgggacatt actcgcagca
     5461 tgtggtgggg gagatagtaa ttctaaagac gggcaagtaa ctttaagcta tgcgagttga
     5521 gatactgacc aagctccagg actaagaaaa atgttagatg aatttgaaaa acaaaatcct
     5581 gatatcaaag tagaaatgga gacaacgcct tgggatcaat attgggttaa attagaagct
     5641 gcaacaacgg gaggtaatat gccagatgta gtaaccatgc actctagcga atcctataaa
     5701 tatatgtcac agaatgcttt gatgggactt gatgatgtta tcaaagaaaa tgatattgac
     5761 atgggaaact ttactgaagg aattgctgat ttctatacgc ttgaagacga actatacgca
     5821 atccctaaag atgcttctgt agtggggcta tggtacaaca aagaattatt tgataatgca
     5881 ggtgtcgatt atccagatga aacatggaca tgggaaacac tcagacaagc tgcaattgat
     5941 ttaacagatg aatcaaaagg catctatggt tttgcatcac atattggaac agagactggc
     6001 tactggccat ttatttatca aaatggggga gaagtatttg ccgatgacaa tacaaaatca
     6061 ggtttgaata ccccagaagc acaagaagct ttagcttttt atcgagatat tgcacttgtt
     6121 gatcatgctt caccaacaat tacagaatta caagatcctg ataagttaag tcgttttcaa
     6181 tcaggtagat tagcaatgat gattgaagga aactggtttg caccgggatt tttgaaaaat
     6241 gactatctag ttgaaaatgt tgcttctgcc gtgttaccta agggaaaagt taatgcaaca
     6301 attacaaatg gacttggttg ggctgccagt gcgagtacaa aatacccaga agaagtgaaa
     6361 aaactaatgg catttttagc tagtgaggac gccaataaaa tccaagcgga aacgggggca
     6421 tcaatcccag cactaaaagg ctttgaagac gagtgggctg cttcttcaga tacaattgat
     6481 ttgagtgctt ttggaaaaat gttggagtat ggagtccctc gtccatacaa taaagatgga
     6541 ttacgagctg aacaagcaga agttcaaatt attaaccgta ttctgtctgg tgaagtaact
     6601 gttgaagatg gaaccgctca aattgaaaaa gaagtcaatg cgatattgag cggaaattaa
     6661 attagttaat tatacaatat acaggcgaaa ggattctatt tcgtctgtat attaatggat
     6721 gaataccaaa aagggggata ttatgaaaaa tcgaattttt ggaaaatatc gtaaagatca
     6781 agtgatatgg ggctatattt ttatttttcc aacggcatta ggattaattt tattaaatat
     6841 atggccagca attcaaacag caattttaag ttttcaaaaa acggtagggt tcggtgatac
     6901 tgaatgggtc ggattaagaa attaccaaaa aatgtttgca gacggtgaag tttttcagtc
     6961 tttattaaac acattaattt atgcagtggt ttcgatacct agtattgttg tattatcctt
     7021 gttggtggca gttttaatga atagaaaagt taaaggattg tcgatttatc ggacaattta
     7081 ttttctacca gtcgttgctg caccatccgc agtcgcaatg atttggcgct ggatgttcaa
     7141 taatgattat ggactgatta ataacatgtt ggctaaaata ggtctcgatg gaccagcatg
     7201 gttaacggat ccatcgattg cgatttattc tattatcatt gtaggagttt ggagtgctat
     7261 tggttataac atggtgttat tactagccgg cttataagaa attcctaaag attattatga
     7321 agcagcggaa attgatggtg ctagtccaat ccagcagttt ttcaacatta cgttaccatt
     7381 agttacacca acattatttt ttgtagttgt aacaacagtt atcaatgctt tccaagtatt
     7441 tgatgttatt tttatgatga ttactcctaa tagtaccgcc atgtttaaat cacaatcatt
     7501 agcctattta ttctataaac attcatttat tttaaatgac aagggatatg gttcggcaat
     7561 cgttatgttc cttttagtga ttatattaat tattactgca attcaaatga agttgcagaa
     7621 aaagtgggta aactattaag gaggctatca aattatgaaa aataaatttt tttcaaatgc
     7681 gccaatccat atcatgctta ttttaggtgg tattgcaatg gcattacctt ttatttggat
     7741 gattttaact tcagggaaaa cgttggttga atctacccaa attccaccta caattttccc
     7801 tgagaaattt cagctaagta actatcaaca agtacttgat ttattaccat tcatggcttt
     7861 ttatatcaat acatttttaa tggttgttgg acgagtgatt ggctcggttt tattttcagc
     7921 aatggctgca tatgcttgtg cgcgtctaag ttttccaggg aaaaatatcc ttttcagctt
     7981 agtacttatt caaatgatga ttcccggttc catttttatt actccacagt atttattagc
     8041 acaaaaattg ggtattttaa atacaattcc agcattaata ttaccagggg tagttagtgc
     8101 gtttggtacg tttttattaa gacaattttt cttaggttta cctgatgaat tggaggaagc
     8161 ggcctattta gaaggtgcca atatttggac gatctttttc aaaattatgt tgccgcttgc
     8221 taaatcagga ttggttgcac ttggaatatt cacagcgttg tttgcattta aagatttaat
     8281 gtggccattg attgtcaata cttcaattga taagatgcct ttagctccgg gattagcaaa
     8341 cttacaagga caatatgcca cgaacttccc acaattaatg gcaggttcgt tattagcaat
     8401 ttggccaata gttgtgatat tcatcgtttt ccaaagaaaa ttcattgaag gaattgctac
     8461 aacaggtagt aaattataaa actattaatt agtgattgga gagtaagtac atgacaaaaa
     8521 ttacatttat tggtgcagga agtactattt ttgcaaaaaa cgtattaggc gatgcgatgt
     8581 taactccaag tctacaagat gcacatattg cattgtacga tattgatgaa aaacgactaa
     8641 aagaatcaaa tttaatgctt aaaaatatta ataaaaattc gaatgaaaat cgtgctgata
     8701 ttgtttctta ttatgatcgc aaagaagcgt taagagaggc tgattttgtt atcaatgcta
     8761 ttcaagtggg tggttataaa ccttcaacag tgattgattt tgatattcct aaaaaatatg
     8821 ggttgcgaca aactattggt gacaccatgg ggattggtgg gatttttaga accctacgga
     8881 cactaccagt aatgttagac tttgctaaag aaatggaaga agtttgtcca aatgcttggt
     8941 tattgaacta tacaaatccg atggcgatgt taaccatggg tgttttaaaa gcgacgagta
     9001 ttaagactgt tggtttatgt catagcgttc aagtatgcgt accagaattg tttgagcatt
     9061 taggaatagc cgatcaatat gacttaaatg actttcaatg gaaaattgca ggcattaatc
     9121 atatggcatt cttgttggaa attacaaaaa atggggaaga tttctatcca acaattcgtc
     9181 agttagctgc agaaaaacca aatccacatc cagattctgt acgttttgaa ttgttgaaac
     9241 gttttggtta ctatattaca gaatcaagtg aacacaatgc agaataccat ccatacttta
     9301 ttaaatcaaa atacccagaa ttgattgacg aattagggat tccgttggat gaatacttac
     9361 gtcgttgtga acaacaaatt aatgattggg aaagcatgcg agaggacatt gtaaataatg
     9421 gggagttaac acatacgaga agccgtgaat atgcttcata tatcatggat tctattacga
     9481 caggaactcc gtcagtgatt gcaggaaatg tcttaaataa aggcttaatc acaaacttac
     9541 cgacagatgt ttgtgtggaa gtaccttgtt tagtcgataa aaatggcgtg caaccaacct
     9601 atgtcggtga attgccaaca caattagctg ctttaaatcg gacgaatatc aatgttcaag
     9661 aactgacagt agaagccgct ttaacactag ataaatcaaa aatttatcaa gcagcgtatt
     9721 tagatccaca tctatcatct gaattgtctt taacagatat caaaaattta gtggatgatt
     9781 taatcgaagc acatggggac tatttacctg aatataatta aaaagaagct gaaagagtaa
     9841 ggaattttca attccttact cttttcttag ttaaatttag taaaaatatt gacaaaacac
     9901 ttagtaaaca gttacattag tgttaacaaa aagaaaacgc attcaaattg tataaggagg
     9961 agcataaaat gaagtggtct aaaaaaatta ttttagcttg tatagcacta ggagcaacga
    10021 tctctttgac tgcttgtgga aaaaatcagg aagatgcaag cggtaaggta gaaatagagt
    10081 tttttaatca aaaaaaagaa atgactcaaa cgattcagga gattgctaaa gattttgaag
    10141 caaaaaaccc tgatattcat gtaaaagttg tggatgtacc gaatgctggt gaagtaatta
    10201 aaacacgtat gcttgcaggc gatgtaccag atgtcattaa tctttatcct caaagtatag
    10261 aattgaaaga atgggcgaaa gctggttatt tagaggactt aaccgaagaa ccgtatttag
    10321 agaatatcaa aaatggatat gcacaacgtt ttgctattga ggatcgtgta tatagcatac
    10381 cattaaccgc taatgtctat ggtttttact ataacaaaac tgcttttgag gaaatgggga
    10441 ttcaagcacc agaaacttgg gcagaatttg aaaaaatagt agctgagatt aaagatcaaa
    10501 acaaagtacc gtttgcgatt gcaggagcag aaggctggac tttaaatggt tatcaccaac
    10561 tagctatagc gactgctgct ggtggtgaag aggaagcgaa caatgtttgg cgtttctcag
    10621 aagttaatgg aattaatgct gaaagcaaag aaatgcaaaa agattttaat cgattagatt
    10681 tgttaagaga accaaaagct ttgcaaaaca actggcaagg tgccggttat aacgatacag
    10741 tagttacttt tacaaaaggt gatgcgttga ttatgccgaa tggttcatgg gcaatgccga
    10801 tgattcattc acaaaatcca gattttgaag taggaacttt tccttttcca gcagatgaag
    10861 caggtcaaag tctaacgatt ggcgcaggag acctagcctt atcagtttct gcaacttctc
    10921 aacataaaga agaagccaaa cgttttgtag aatatatgac aacacctgaa gcgatgcaga
    10981 aatattatga tgtagatggt gcaccgtgtg ctgttgaggg cgttatcgag aatactcagg
    11041 attcaccatt aagtggttta actgaacttg cttttactga tcgtcattta gtttggttag
    11101 ccaaagattg gaatagtgaa aatgattttt ataccttaac aacaaattat ttacacaatg
    11161 gcaatcaaaa agccatgatt aacgcattaa atgctttctt taatccaatg aaagctgacg
    11221 gaaggtagga aaataaaatg aaaataaaag aaattttaaa tcgttactgg gcaatcattt
    11281 ttgtagcaat tccaattatt ttgcagatga tttttttcta ctttccaatg gttcaaggtg
    11341 ctttttacag tttaactaat tggactggtc tgacttataa ttttgatttt gtgggtttga
    11401 acaattataa gattcttttt tctgatccga agtttatcaa atcgattggc ttcactttaa
    11461 ttgtgacgct ttgcatgatt gctggacaaa ttggttttgg tattttgatt gcgcgggcgt
    11521 tgaacgcaaa tattaaaggg aaaacattct ttagagcatg gttctttttt ccagcggtct
    11581 tatcgggcgt ggttgtttct ttagtattta aacaaatttt taactatggt ttaccaaaaa
    11641 ttggtagtat gttaggcatt gaatttttac aaaccagtct tttaggaaca acatggggag
    11701 cgatttttgc agtaattttt gttttacttt ggcaaggaat tgccatgcca gtcatcattt
    11761 tcttagcggg ttttcaaagt attccacaag aaattattga agctgctaga atggatgggg
    11821 cgaatgataa gcaaattttt tggaatattg aaatcaccta cttattacca agtatttcaa
    11881 tggtatttat tatggcatta aaaggtggct taacagcatt tgatcaaatt tttgctttaa
    11941 ctggtggtgg accaagcgat gccacaacat ctttggggtt acttgtttat aattatgctt
    12001 ttaaaaacaa tcagtttggt tatgcgaatg ctattgcttt agtcttattt atcttaattg
    12061 ggattgtttc actggcacaa ttaaaattat cgaaaaaatt tgaaatctag gaggtttccc
    12121 aatgaaaaca tcaaaagaaa aaaatatttc taaatacttg cttttattat tagggggctt
    12181 catgatttta gttcccttag tttcaacgct attcggttca tttaaaacaa cacccgatat
    12241 tatgaataat ttctttcgtt ttcctaatcc agcgactttg gaaaatttcc gaagattgtt
    12301 agcggatgga attggtcatt acttttggaa ttcggcaatc atcacaatca ttgcagtagt
    12361 tgctgtgaca gcaattattc ctatggctgc ttattcacta gcaagaaaaa tgactcgtgg
    12421 gaaagcatac ggattgatgt atttattttt aattttagga atttttgttc ctttccaagt
    12481 aattatgatt ccgattaccg ttatgatgag taaaattggt ttagcaaata tgtggggatt
    12541 aatcttactc tatctcactt atgccgtacc acagacacta tttttatatg ttggttttat
    12601 taaactaaat gtgcctatca gtctggatga ggctgctgaa attgatgggg ctagtcactt
    12661 tacaacttat ttcaaaattg ttttcccaat gttaaaacca atgcatgcga cgactattat
    12721 tcttaatgct ctatggtttt ggaatgattt catgctacca ttattaattt taaatcgtga
    12781 ttctaatatg tggacactac cattgtttca atataattat acgggacaat attttaacga
    12841 ctatggtcca agttttgctt cctatacagt aggaattgtc acgattacac tagtttatct
    12901 tgctttccaa agaagtatta tagctggaat gagcaatgga gcagtgaaat aaccaataaa
    12961 ttattctcat tataaaggag ttcataaact tatgattata tttgacaatc acacacaaac
    13021 atttcattta caaaataaaa aaattagcta tctattagga atcgaagaaa ataattattt
    13081 aacgcatctt tattttggaa aaaagattac gcactattct ggtgggtatc gctacccccg
    13141 aacagatcgt tccttctcac ccaacaatgc aaatgcgcaa gatcgtttat tatctttaga
    13201 cacattacca ttagaatttc caggatatgg acatggtgat tttagagaac cggcgcataa
    13261 tattaaatta ggaaacggta gtcgtgtaaa tgattttcgt tatgatagct acgaaattca
    13321 aaaaggaaag cccagcttag cgggattgcc agccttatat acggaagatg atgatgaagc
    13381 tgaaacgctg attattcaat tgaaagacgc gattagtgga ttgactttac agttgttcta
    13441 cacaatttat caaaatcaca gtgtaattat tcgttctacg caattagtaa ataatagtga
    13501 tgaaacagtg gagattaacc gcttagctag tcaatcaatt gattttccaa atcgtccact
    13561 cgagttaatt catttaaatg gtacctgggc aagagaacgt caaatgacta gagaaaaaat
    13621 tcatacaggg ataaaagtat tagatagtaa acgcggatca agcagccatc aacaaaatcc
    13681 gtttgttgct ttagtagatg ccaaaacaga tgaatttcaa ggagaggcat atggatttaa
    13741 tttagtttat agcggtaatc acgaaacggt gattcaaaaa gatccatttg accaaacacg
    13801 ggtcattatg ggaattaatt catttaattt tggttggcaa ctaaaaactg gcgactcatt
    13861 tcaaacgcca gaagtcgtga tggtttatag taatgaaggt ctgaatacgt tatcacagac
    13921 gtatcatgaa ttgtataatc accgtttaat tcgtggaaac tatcgtttaa aagaacgtcc
    13981 cacgttaatc aacaactggg aagcaacgta ttttgatttt aacgaagaaa aaatcatgaa
    14041 tattgtcgat gatgcgaaga acttgggcat tgaaatgttt gttttagatg atggttggtt
    14101 tggcggtcgt gaaaatgact tcacttcatt aggtgattgg gttgaaacag aaggcaaact
    14161 tgcttgtggt ttggaaaatt tagccaagca agtgcatgat aacgggatga aatttgggat
    14221 ttggtttgaa ccggaaatga tttctgaaga tagtgaacta tttcgagcac atccagattg
    14281 ggcattgggt attccaggtc gtgaccgctc aattagtcga aatcaatatg ttcttgattt
    14341 ttcaagacaa gatgttcgtg ataatatcta tcaacaaatg acggcgattt ttgatcgtgt
    14401 accgattgat tatgtgaagt gggatatgaa tcgaaatatg acagaagtct attctgtctt
    14461 gttggactca acgatgcaag gtgaagtggc tcatcgttat atgttagggt tatatgaatt
    14521 tttagaaaag ttaacaacga attatccaac tattttattt gaaagttgtt cgggtggagg
    14581 cggtcgtttt gatgcaggtt tcttgtatta catgccacaa acttggacga gcgacaatac
    14641 cgatgccgtg gctcgtctga aaattcaata tggaaccagc ttagtgtatc caatttcaag
    14701 tatgggtgcg catgtttcag cagtcccaaa tcatcaaaca ggaagagaaa cgagtttaac
    14761 tattcgtggc aatgctgcga tggctggtgt ttttggttat gagctagatc taacgcaatt
    14821 aacttctgaa gaaaaagcag aaattaaaga acaagttgcc ttttataaaa agcatcgcca
    14881 acttttgcaa tatggaactt tttatcgctt ggaaagtccg tttgaaggca atgatactgc
    14941 atggatgttt gtctcaaaag accaaacaga agccatggtc ttttatttcc gagtattagc
    15001 agaagctgcg catccattag tcacattgaa attagcagga ttagcagaag aagcaactta
    15061 tcaaatggaa gatgtaacat ttgctggcga tgaattgatg aatctcggtt tttatattga
    15121 ctcagaacta catggtgatt atgcgacaca acgcttttat ttgaaacaga tatagtagct
    15181 tgaatttaca atataagtgc aacaccaaaa gagactcatt ccaaacgaag aaaatatgtt
    15241 ttatttacac gcatttacta aaaagcagcc tgatttgaat tgggaaaatg aagaagtacg
    15301 tgaagaaatc tatgcaatga ttaactattg gttagataaa ggactaggcg gatttagaat
    15361 cgatgcgata ttaaacatca aaaaaaaact agagtatggc atctttgaat ctgatggaga
    15421 agatgggtta gccttcattg ggccctggat tcttaatcag tcgggcattg aagtgtggtt
    15481 aaaagaaatg agagagcgga catttaaacc acataatagt atgacggtag cagaagccga
    15541 tgttcctaat gaacgtttag atgaatatat tggtgaagat ggctattatt cgatggtttt
    15601 tgattttagt tatacggata ttgatgtacc tgaaactggt gaatggttta aagatagcca
    15661 atggacatgg acggatatgc gtaccaatat ttttacgaat caattagtta cgcaagataa
    15721 aggttggggc gccttatatt tagaaaacca cgatcaacca cgttcaatta ataaatatat
    15781 tccagaagaa tggatcaatg attatagtaa aaaaatgttg gcgacgcttt ttatgttact
    15841 aagaggtacc ccttttattt atcaaggaca agaactagga atgaccaaca tagaaatggc
    15901 atcattagaa gattttgatg atgtagctac gcattctcag tataaacggg cattagaata
    15961 tggattggca ccagagcaag cattgaaagc tgtcaataaa cgaagtcggg ataattctcg
    16021 cacacctatg caatggaata cgcaaaaaaa tgccagattc tcaacttctg ataatgtatg
    16081 gttaaaagta aatcctaatt atccagaact caatgcagaa gctcagttaa ccaatcaaca
    16141 gtcggtattc aattattatc atcaattaat taaattaaga aaaaatagtg tatacaaaga
    16201 cgtgctaatc tacggacaat ttctaccagt gaaagataaa aatgaacata ttttgcttta
    16261 tgaacgccaa ttagaagata aaatcgtatt agttatctta aacgttacag caacagaaca
    16321 agaatattat gtagatgaac agtaccaaca agtattaatc aataattatg aagatgtttt
    16381 attagatcaa agtcgaaaat tgcgtttacg accgtttgaa agtattgtat tagctaatta
    16441 tggaggacaa ttatgaaaat taaaaacgaa gcaatgctca tcacctatgc agatagttta
    16501 ggaaaaaaca tgggcgaatt aaaacaagtc ttagacaaac atttacaagg tgttattggt
    16561 ggtgtacatc tgttgccttt ttttccatcg acaggtgatc gaggctttgc gccgagtgat
    16621 tatacgacag ttgatccagc tcttggaaca tgggaagatg tggaagcatt aggtgaagat
    16681 tattatttga tgtttgattt tatgattaat cacatttctc gtgaatcaaa atttttccaa
    16741 gatttcaaaa aaaaccatga gcagtcacca tacaaagaaa tgtttatccg tattcatgaa
    16801 tttttcccag aaaatcgacc aacacaagaa gatattgatt taatctacaa gcgtaaagac
    16861 aaagcgccgt ttcaaacggt agaatttgcg gatggtacga cagaagaggt ttggaatacc
    16921 tttggggaag aacaaattga tttagatgtg accaaagaag ttgtgaaaga atttattcga
    16981 gaaaccatca aagatatggc gaaccatggc tgttctttaa ttcgattaga tgcgtttgct
    17041 tatgcgatta aaaaattaga taccaatgat ttcttcgtgg aaccagatat ttgggaactt
    17101 ttagacgaag tgcgtcaaga agcagcgaaa tatgacgtcg aattattacc agaaatccat
    17161 gagcattatt ccattcaaat gaaaattgcc aatcatgatt attatatcta tgacttcgcc
    17221 ttgccaatgg tcactctgta ttcactttat agtggaaaat cagaacgttt agccaattgg
    17281 ttgaaaatga gtccgatgaa gcagtttaca acattggata cgcatgatgg aattggtgtt
    17341 gtggacgctc gtgatttatt aacagatgaa gaattggatt acacctctga ggaattgtat
    17401 aaagtcgggg cgaatgtgaa gaaggtctat tcaagtgcca attacaataa tttagatatt
    17461 tatcaaatta acagcaccta ttatagtgca ttaggcgata atgaccgtag ctatctgtta
    17521 gcacgtgcga ttcaagtatt tgcgccaggg attccacaaa tttattacgt tgggttatta
    17581 gcaggaaaaa atgatattga actgttggaa gcaacgaaag aagggcgtaa tattaatcgt
    17641 cattactatg atttagccga aattgaagaa gaagtgcaac ggccagtgat tcaacaatta
    17701 tttgcacttt taaaatttag aaatgaatct gcggcgtttg atttggatgg aacgattgaa
    17761 gtagcaacgc catcagaatc agaaatagtg attacacgta aaaataaaaa cggtagtgat
    17821 agtgcaacat taacggctaa cttggatacg aaagaattta tcattgagtc taaagatata
    17881 aaaattttca ctagtgttgc attaaaattt taacctattc catagaacaa aaaatccttt
    17941 atactagcac ttggatgact tatccaagac caataaaaag gactcataca tggataagta
    18001 taaaacaaat acagcattta ataaatggtt ttctgccata aaattaggaa aactttctgt
    18061 caactcccaa aaatgtattt ttgattacaa ttcgtatcgt aaaaaactgt cttttgaagc
    18121 tgtgctcaag ctctttctct acgcagttaa tgaagaaaaa gaaagtttgc gtgatttgag
    18181 tacagctctt atcgaccgat cgctccaact agaaaccgat gtgtcatcga ttagtcatac
    18241 ccaattgtct cgagcattta atgcacttga acctaaagtg ttagaagaaa tttttcaaca
    18301 actgttagaa aaagttcaac atcaaatcaa accaaccaag cgaaatcatc tgtatctgat
    18361 tgattccagt acgttttcgc ttagtttaaa acgtcataag tgggcgactt tccgtcaaac
    18421 aaaatcaggt gtcaagttac acttaaacta ttgttatatg gacgatacta cgatgtatcc
    18481 aactgatttt accttaacaa atgcccatga acatgatctc aatcaactac cagtattggt
    18541 gaatcaacca gaggcaactt acgtgtttga tcgtgggtat taggactttg aaaagatgga
    18601 tcagatgcat tgggatggct atttcttcgt cactcggatt aaaaaaaaca ccaaagttca
    18661 cgtgatagat actctggaaa catcgccaga aacagaaatt ctacgagacg agctggttcg
    18721 tttaggatct aaaacctatc taaccgccaa tttccgacta gtcactgttc aagataagaa
    18781 cggacgtgtg tttcaattta tcaccaatcg aatggacgtc tcttcaaaag aaatctctga
    18841 tatgtatcac gctcgttggc aaatcgaatt gtttttcaag cacatcaaac aacacatgac
    18901 cattaaaacc ttcttttctc aaagtgaaaa aggcgtgcag aatcaattga ttttaacgat
    18961 gatttccgct ctcctcacgt ttctcatcaa attggagacg aaaacggaaa aatcagtgtt
    19021 ccaaatcaaa cgattctttc gttatttact gtttcagccc tttgaatgtt gtctagaaaa
    19081 attaattccc acatgagaaa aaagaaattt ggctcgttac ttttggtttt cagttgaata
    19141 acagataaaa aattttctgg aaaaagtcat cctctgcgtc agcacagttt tttttgactt
    19201 attttcagag gaaatagttt tcagaatatt ctaaaatgtt ttatgcaaca ctagtgatat
    19261 tttttagaat tagatttcta atgtgcattt tttgagtatt aaggagcaag tatacaaaga
    19321 tttataaagc tgattaaatt tgaataaaaa tatgcaggaa cgctactatt tttagtagtg
    19381 ttctttttta tatttgaata aaaaatgtgg tggttaaatg gtggttataa caaaaaatga
    19441 attaatgaaa ttaggttatg ggaagtatca gtctgagaga atcatacgac aggcaaaagc
    19501 aattatggtt tcaaaaagct atacctatta cgaaaataga agattaggac tagtacctgt
    19561 tgaatctgta gaagaagttt taggtatgaa gatagaggta gaggaggatt ttcagaatca
    19621 tggttaattt taaaaaagat aaaaatggga aatatcattt tactgctagt ttgggattcg
    19681 acaaacgaac agggaaaaga gtacaaaaaa acgtagaggg gttaaaacag ttaaagaaga
    19741 aaaaaagcgt ataccgaact tgtttctaat tatggaaaag aagcagaaac ttctcaaagt
    19801 actatgttgt ttgaggagtt tttttataaa ttattcctac cttattatca agggaaagta
    19861 gaagaaagaa catataaaaa tcggttatct acaatcaaaa aaacttttct tatttcttta
    19921 aaatggagtt aagagaaata cgtccaattg acattcaaaa gtggcaaaat aatttattga
    19981 gcaaatataa aaattcttat gtaagaaaca tgtatgggat ttttcagatg tcattagatc
    20041 gagcagttgt tttgggacta cttgaaaaaa atcctgcaaa agttattgga aatgtgaaga
    20101 aaaggaaaac tacagttgat ttttggacaa aagaagaatt tacaaaagtt atttcaactt
    20161 tttatattga tgatttgtac caacattttg gttttatttg tatgtggtta ctttttatga
    20221 ccgttatgcg tttgggagaa gccactgcat taacttggaa agatattgat tttgaaaata
    20281 aaaacttgcg tgtgaataaa acactctatt ataagagagc caaaacctat gagtttgtag
    20341 aacccgaaac ggcttctagt attcgtacaa tcgaactaga cgacaataca attacctttt
    20401 taaaagcttg gaaggagcgt caagtttcaa taggaggcat gtcgtttgta ctttcttata
    20461 atggaacacc tactcagaaa tatacaatcc gtcatattat gagaagacat gcaacgatcg
    20521 ttggtgttca tataatccgc atacacggat taaggcatag tcatgcttct ttgcttatat
    20581 cacaataaaa agatgcactt cgaattaaag aaaggttaaa acatgctgat gtacaaacga
    20641 ctttaggaac ttatggtcat ttgtatctaa attcattgag agagataaca aatgatccca
    20701 cagattatat ccaaactaat ttttctaaaa tcaaaccgac acctatttac gataataaaa
    20761 aataaatgat aaacaaaagt aggatcacac caaaagaaac acactagaaa aactataata
    20821 caggcttttc tagcgtgttg tttcttattt ccgcctattt atacattata ctcataaagc
    20881 atttaatgca tcgatcgtag ccgcattgtc tatatgggta tataaagttg ttgtattggt
    20941 acttgaatgt cccaattgtt gggcagttaa taccaatgaa tttgtctgtt ggtagagtct
    21001 agtagctaat gtatgacgca gtttgtgagg gctgacttga attctaaagc cttttgaata
    21061 ttttgctacc atccgttcga ttgcaatacc ggatatacgc ttggcttctc cttggtaaat
    21121 tgctagaaat aaagcttttt cgttttctgt tactttatat cgttctttcc gaatagctag
    21181 ataattagct aaatattcca ttgcgaatgc tgctatattg acagcatctc ttttatttcc
    21241 ttttctagtt actaccacat tattcttatc taaagatagg tcatctaaat taatatttat
    21301 aacctcggag agtctcaatc cagatcctaa aatcagtgca ttgatcgcca ggtctctttc
    21361 tacatctcgt tgataataga tcaaagctcg tttactcaaa gtatttttat attcattggc
    21421 aatataatct agataatttt gtgtatcctt atctagaaat aattttttct caatggcatt
    21481 ggcacgtgca gctaacgtag ttttatctgt gatggttgct acttgatcca taggattatt
    21541 gtctaagtat ggttttccta gaggattatt ggaacttttc gaaagatatt taaatagaac
    21601 ctttaatgcc gtgatatttc tattgatagt agaagtagat agacctaagt tattattatc
    21661 tttgagctta gtttttgttg tctctttttt tacattttct tttgtgcgac gtttcaaatg
    21721 agatttgtac aagtttaggt cataggcagt taactgtctt atcgaggtga tagaagtatc
    21781 aggaacgata gactcactca gaagccattc ataaaagagc ttataatttt ttagatattc
    21841 taaagtggtt cgaggtgatt gatctttatc ctcacgatct tgaacaaaat cttttatata
    21901 atcgggcata tgaagtatga ttgtatctat tttttgatat atttctgtat ttttcatgtc
    21961 attcatcctt tcaataagta ggggagaaga gtatctctat aattggacta taccaattat
    22021 agaatgtttt gtcaattggt atgctctaaa atttattaat ttgtttcaaa aaaagcatat
    22081 aaacaggtat acgtttaata atttaaaata aatggtatac ataaataggg aaggaaatag
    22141 aagggagcaa tctaaatttt ttgaattttc ttaattaatt atatagaaag aacaatcttt
    22201 ctatagagtt agacatgaga gggactatgc ttgatttaaa atcaacaagc gtatatactg
    22261 tatatacaaa gaaaaaggag ggagaagact atggcaatta aagaaagaaa atttagggaa
    22321 gtggggaact cagtagtatt aactatttca aaggagtttc ttaaagaaaa tgggttaaat
    22381 cccggagata cggttttttt agatgaagaa aaacttcgag cggcaattac aaaaaaacaa
    22441 gccttatctg atgaaattga tttagcaata tctcaagcat taactgaata tgatactact
    22501 tttagagagc ttgtcgataa atgagtattc attacctaga agccaaagat attataaaaa
    22561 tgaatgtaat ccaaataaaa aaatactctc ctaatgaacc ccttggtgta aaggatgcga
    22621 atgctttaga aatgtgtgta gggcaacttc gagcctcagc ttttggtgaa gaggtttatc
    22681 cttcgattta tgaaaaagca gctatcctac ttatacaatt gataaagaaa catccttttc
    22741 acaatgcaaa taaacgtact gctttcttag ctacttttgt ctttttgaaa atcaatggac
    22801 agcttttagc tattgatcaa aaagatgctg taaatttagc ggtatatatt gctacgtatg
    22861 ataaggattt cgatcaacta aaatatgaag tgatcaacac tataaagaca catacgcagc
    22921 ccatttaagg agttactgta tgtgtcttta tagggttatt agctgctata gcgaaataat
    22981 tgaagtaaat tacaaatagc tataattcaa atccaactat atagaaaacg taaattttct
    23041 atatagttgg attaagataa cgactattta ttttatcata tttttactat accctatact
    23101 tttacttggt atatttatga ataatcgcaa atttacccct atactactag tttgtaagtt
    23161 agattaataa aaatactaaa gttatttgtt cttttattaa atacacctac aacttataaa
    23221 ttgatggtat ttagataaca cgaaagaata aaaataacag ctgatagtaa agtttgttga
    23281 cgattgctac tcaatcgaaa gtgagttagt gcgtatgaac ttgcttctga aaacttatga
    23341 aatgaaagac cttttacatc ttgcagaaat cggtactggg attaaactaa gcaataaaaa
    23401 ctaatgttcc cctcattctt tgaacttcta ctatttgatt tatttattct gataaatgac
    23461 gatttcacct tcaggatctt caaatcccgt atagaattta tttaagatct cttcagtatc
    23521 agtaatagga taggcgatag gccacatacc ctttgatggt tagaataaac tgctcaaaat
    23581 ctgtatcgta tgttggcttt agcatattaa caaaggctat ctccaaatta taaaccataa
    23641 gcgttgaatc gttcttaaca atttcaattc ttcctaaccc aaagtcagaa gatataatta
    23701 ccggacttgc cttacccttc atgtgggttg ttgagattcc atatttaaag ggaattgtcg
    23761 gtactatcgg cggtctgaaa aacatcccaa aaataaaaaa atctttcccc tgctagtctc
    23821 ctaagtccct ttgtagcgga tatggcacca taatctcatt ttatcgaagt gagtcatggt
    23881 ttatctgttg gccaaccaaa attgatacaa tctaacgatg caccctctac ggcattctag
    23941 gggagtgcac ttgcattcac ccaataaaaa ccggcagtta gcaaagaact tatgtagtcg
    24001 tatgttctta ctgttaacat acaagaggtc tcctttccac aattgcttag cagtaaacgg
    24061 gaatttacaa agctaatctc ttccattttc ttatctttgt tctaaaagtc ccaaatggag
    24121 caactgtatt tacatgaata aatttatata cttcccatac agccgttttg gttgcctcgt
    24181 ctgcccattt tcttttgtgt gcagtgaata gatcttcatc actcattgaa tcaatcatca
    24241 tgttaatatc atcaatatta cgattgagaa tttcctctaa ttctttcaat ggtagatgtg
    24301 cataggtatt tgtaaaccac ttatataact ctcctaactg attccattta aattgatcag
    24361 aaggcgtttt aacagataat ccatttcttt catcattttc ccattttaga attaaattcg
    24421 tccaaccaac ttgatatgct aaattttcag caggtgttct atccacttcc tcaactcttt
    24481 tatccttcaa ttcttcagga atagtttcaa attctaaaat atatttagta taagtcctat
    24541 ttatttcttt ttttaattct tctttatttt tatatgttct cattatatta cctccataaa
    24601 ttcctaattg tctcctttaa ttcatctata atttcatcca cgatatttta atccgttcta
    24661 caatgcatat ctgatatgca ttgactgtct aactcatatg tgaatgattt ataaaatatc
    24721 ccaacgtctc atctaaagaa atgttgatcg tcagcaagga gctaaaaaat acgaatatat
    24781 ttcttttgta gtaaaatcgg actggtttgc atatcatttt cttcgtaaat atcaaaatca
    24841 tgttaactac tatcaattac taataaaaac taccacttta agcgttaaga actgttaagt
    24901 ttagttaagt gaggatagtt cacaaaatcc attgttaaac ttcacgaaag tggtaaaact
    24961 caaaatgctt tatataggat tacagcatcg ctctttcttc gattgcgcgt tgggtaaaaa
    25021 atattatgaa gtaaaaattg acgatgacac gttccttacg gctcatcaga tgaaacagct
    25081 acaaaaacga aatgatcaac tcgaagaaaa tttaattcta aaaaagcgat tgccatattc
    25141 acgcctcgct cgacgaacta ttaaaggtca agatttacct gaagatcatc ctttttagga
    25201 aattgaagct ttttatgttg cacaatgcat ttataatact acaatgatgt tttcaccaga
    25261 tattcttcta gggtgcggtg taatgaaaca gatacacttg caaaagaaaa ttcaccgcga
    25321 atttactaaa attatgaata attatgttaa tcttccgaaa tttttttcca aaattaaggg
    25381 atcaagctgg tgttatagat tgtttaaggt tggcaaaaaa ataacttctt tttaaacaat
    25441 tatagaaaac cgatactctg ttaagttggt tgacacggtg tcgattttta tgactttctt
    25501 attctgaaac ttcactaacg gacagtacct aaaaagtcta tagtatggac gtttacgagt
    25561 actattttat caaaaatgct gctcatttca acttatatgt tttagtgaac tacccccact
    25621 tagc
//
LOCUS       DAZKBH010000026        25463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000026 DAZKBH010000000
VERSION     DAZKBH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.026"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..2198
                     /locus_tag="ACG8OO_001100"
     CDS             138..2198
                     /locus_tag="ACG8OO_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFP9044991.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2281..2850
                     /locus_tag="ACG8OO_001101"
     CDS             2281..2850
                     /locus_tag="ACG8OO_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9044992.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3025..3831)
                     /locus_tag="ACG8OO_001102"
     CDS             complement(3025..3831)
                     /locus_tag="ACG8OO_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9044993.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3969..5483
                     /locus_tag="ACG8OO_001103"
     CDS             3969..5483
                     /locus_tag="ACG8OO_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFP9044994.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5886..6869)
                     /locus_tag="ACG8OO_001104"
     CDS             complement(5886..6869)
                     /locus_tag="ACG8OO_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFP9044995.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6874..7521)
                     /locus_tag="ACG8OO_001105"
     CDS             complement(6874..7521)
                     /locus_tag="ACG8OO_001105"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9044996.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7606..8238)
                     /locus_tag="ACG8OO_001106"
     CDS             complement(7606..8238)
                     /locus_tag="ACG8OO_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9044997.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8254..8805)
                     /gene="coaC"
                     /locus_tag="ACG8OO_001107"
     CDS             complement(8254..8805)
                     /gene="coaC"
                     /locus_tag="ACG8OO_001107"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFP9044998.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8802..9539)
                     /locus_tag="ACG8OO_001108"
     CDS             complement(8802..9539)
                     /locus_tag="ACG8OO_001108"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFP9044999.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9536..10525)
                     /locus_tag="ACG8OO_001109"
     CDS             complement(9536..10525)
                     /locus_tag="ACG8OO_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9045000.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10783..11325
                     /locus_tag="ACG8OO_001110"
     CDS             10783..11325
                     /locus_tag="ACG8OO_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9045001.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11342..12427
                     /locus_tag="ACG8OO_001111"
     CDS             11342..12427
                     /locus_tag="ACG8OO_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9045002.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12439..13245
                     /locus_tag="ACG8OO_001112"
     CDS             12439..13245
                     /locus_tag="ACG8OO_001112"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9045003.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13246..14073
                     /locus_tag="ACG8OO_001113"
     CDS             13246..14073
                     /locus_tag="ACG8OO_001113"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9045004.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14070..15143
                     /locus_tag="ACG8OO_001114"
     CDS             14070..15143
                     /locus_tag="ACG8OO_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9045005.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15571..18267
                     /locus_tag="ACG8OO_001115"
     CDS             15571..18267
                     /locus_tag="ACG8OO_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9045006.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18332..18832
                     /locus_tag="ACG8OO_001116"
     CDS             18332..18832
                     /locus_tag="ACG8OO_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9045007.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18844..19338
                     /locus_tag="ACG8OO_001117"
     CDS             18844..19338
                     /locus_tag="ACG8OO_001117"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9045008.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19359..20168
                     /locus_tag="ACG8OO_001118"
     CDS             19359..20168
                     /locus_tag="ACG8OO_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9045009.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20165..20986
                     /locus_tag="ACG8OO_001119"
     CDS             20165..20986
                     /locus_tag="ACG8OO_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9045010.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21037..23136
                     /locus_tag="ACG8OO_001120"
     CDS             21037..23136
                     /locus_tag="ACG8OO_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFP9045011.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23133..24251
                     /locus_tag="ACG8OO_001121"
     CDS             23133..24251
                     /locus_tag="ACG8OO_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFP9045012.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24290..25168
                     /locus_tag="ACG8OO_001122"
     CDS             24290..25168
                     /locus_tag="ACG8OO_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9045013.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25314..>25463
                     /locus_tag="ACG8OO_001123"
     CDS             25314..>25463
                     /locus_tag="ACG8OO_001123"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9045014.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFR"
BASE COUNT         8045 a         4440 c         5396 g         7582 t
ORIGIN      
        1 ttttcttgaa acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga
       61 ttttatttat ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta
      121 gggaggacta agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt
      181 gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca
      241 cgatgctgat cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac
      301 aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta
      361 ctcatacttt cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata
      421 aagtcagtta tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga
      481 catcggaaac cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa
      541 aaggtgatca gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg
      601 acggtggaag cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa
      661 agaaaatcga tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga
      721 agaacgcaga tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg
      781 tggatacgac taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt
      841 taaaaacaat gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag
      901 gaaaagatct tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg
      961 taacaaaaag cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag
     1021 atactacaac tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa
     1081 atgtggcaga aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca
     1141 tcactcattt tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta
     1201 gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata
     1261 tagatttatc gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag
     1321 caaacggatt agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac
     1381 tgatgaataa taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag
     1441 acaatcgttc attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag
     1501 aagagttgat ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat
     1561 cggttacaat caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt
     1621 ttacgattat gtatagttat catgacccgt attcactcca agaactccat gctcagtcac
     1681 tattacatgt tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacgagaag
     1741 aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa
     1801 aacaaacaag aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa
     1861 cgataaggca agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag
     1921 agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga
     1981 aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg
     2041 atgagcatct gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag
     2101 gtattccttc ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg
     2161 ctaatcgaaa tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag
     2221 tcaaataagt agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa
     2281 atgttagata aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat
     2341 gttccaatca cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa
     2401 tctgcttggg cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt
     2461 gagtattttc aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt
     2521 gtagagacaa aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga
     2581 gagcaagtgt atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg
     2641 ctcgcaaaag cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga
     2701 ccaatctatc ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg
     2761 ggttttactt ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt
     2821 atgcaggttt taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg
     2881 gataaaaaac gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata
     2941 actaatggca tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc
     3001 ccttctcttg ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat
     3061 gaacgtaaat ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac
     3121 tgctccaata aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta
     3181 accattttgg atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt
     3241 ccgagtgata tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt
     3301 gatttgctgg atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa
     3361 ttccacaaac tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag
     3421 taataaagga gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa
     3481 gccgacaccc ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa
     3541 atagccataa aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa
     3601 aatgatttct agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat
     3661 ttcaaataaa attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac
     3721 ctgatccact agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat
     3781 tctacctgca gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact
     3841 ccttcatgta tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta
     3901 aagaaaaaca gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg
     3961 ggatacaaat gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga
     4021 gcgcatgcgg cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg
     4081 ccgaagtatc atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat
     4141 cttatgatgt cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa
     4201 aagatgctgg gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat
     4261 ctaaatcatc aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca
     4321 aagactcgaa tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata
     4381 aagaaatgct tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa
     4441 acggtatcaa actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc
     4501 gtccgtctga tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc
     4561 atgagcggga aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg
     4621 aaacggtcaa tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa
     4681 aagcaatcgt agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc
     4741 gtcccgattt agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca
     4801 ccatgattga aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa
     4861 ctgtgcagca ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct
     4921 tggtcgatca agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg
     4981 cagcaattac agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg
     5041 atcgtgccac agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa
     5101 tggaagaatt agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg
     5161 gatggaatac ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg
     5221 aacatgactt atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca
     5281 cgatgggcgg tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa
     5341 ttagggggtt atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg
     5401 gtggaaattc agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc
     5461 gatttgcttc ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg
     5521 ttttgagcta ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa
     5581 agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac
     5641 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg gaaaaagagc
     5701 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
     5761 cagaagaaat tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt
     5821 gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt ttgatcagca
     5881 tgattttatt cagttatctt caaaatgatt tttccgatgt ttttgtttgc ttccatatat
     5941 tcatgcgctt gtttgactgc gctgaattgg aatgtacggt caatgattgg ccgaatcttg
     6001 ccattttcta gaaaaggccc agctttttgc ataaaagttt gtgcgagctc tgctttatac
     6061 tgatcacttc taggtgtcaa taatgtacca agcagttgga cacgtttccc aatcaataca
     6121 ccaaggtcta tctcaggcac gatggtccca cctagcatac caatcaagac ccaacgtcca
     6181 tcaacggcta tagaagctaa attcttttcc cagtaagaag cgccgatgaa atccaaaata
     6241 acatcgactc cccgaccttc agtgattttt cgtacctctt cttgaaagtc ttgtttttta
     6301 tagttgatca attcatctgc accaagttct ttacaaaagg cgagtttttc ttctgatcct
     6361 gcagtaacga tgattttcgc gtgagaaagc tgtctagcta attggatcgc tgctgttccc
     6421 acaccacttg ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt tccaagccag
     6481 taaagtgttt gataagcagt caaaaaaact tctgagattc cagcagcaga aacatagtct
     6541 agcgtatcag ggagaaggat tgcacgatcg gcaggcatca cagcatattc ggcataacca
     6601 cctagattga ctaatccgta tacttttgtc ccaggagtaa aagtggagat atcactttta
     6661 ttctcaacta cctctccagc tacttcgact cccaaaatag ggtagggagg tttcaaagag
     6721 actgattctc ttttcataat atctgttcga ttgacagctg ctgtatggac tttgatcagt
     6781 aattcgcctt gttttggttt aggaacaggc acttcaattt gttctaatac atctacagaa
     6841 ccaggatgct tgattgaaat ggcacgcatg gatctattcc tcctttgccc attcttggaa
     6901 tgctgtattc aactctttgc taccttcaga aagtactaga gtcgtacgag aaatatccgt
     6961 ttcttccact agagcggcaa gtacagcagc aactgtagag cgagtgatcg tttttgatgc
     7021 cttctcatta ggcttagtta attgaatgga agtaacttct tcatcgttag tcaatgtgac
     7081 tggttgaaga atgacaaaat ctaagttagt tcgtttcagc cattcatctg cataatgttt
     7141 agtaatataa taatctgtca tggactcagg ccattttgct ggttcatctg cataaacagc
     7201 actgaccatc agatagcgag agacatgcgc attttcagct gcgatcactg ttttgattgc
     7261 gccatccaag tctacttgca gaagattctt tccttgagag ccggcagcaa aaatcaatac
     7321 atcgatgcct ttgaatgctt cggtcatttt ttccacggac caagttaaat caaaagagac
     7381 atacgaaaca ttctcttttt ttactaatgt ttgggaagca acatctctca cccctgcaaa
     7441 aatttcatgg gaacttggag ctaaatcttt tatcaggtgt cggccaattt gtccgtttgc
     7501 acctacaaca aaaattttca tttggatcat cctttcgacg tcaaatcttt ccattcaata
     7561 gtaacaccaa aagagaaaaa aatcagagat cggctacgat gactatcagt tagtaagaga
     7621 agggtgcttg aagtatctta acaagacatt gcatactgct gcagtcgcaa gacctgcgac
     7681 gattccttca ggtataccac ttgtaaaaat aaccgctaga atagctgaat aaacagcatt
     7741 taaatttgtt ccgatgactt tggcataatc ttgctggaat aaaaagtaga tcatattcat
     7801 cactaaaatc gtattggtca atgaaccgga aagtccagct aagaataaag aaacggaatc
     7861 tgctttttgt ttcattagtt tgcgcagacc tttataaacg aaataaggaa ccaccccaat
     7921 caagatgcga ggaataaacg cgatcagcaa ggctttccag cttccatgtt ctgttccgat
     7981 gactgggata aacggagaaa agacaaaaga caaaggtgtt tggataactg tgctacggat
     8041 catgctgatc agtccaaatg taccgccaag aaaagcacct agtcttggtc ctaaaacgat
     8101 cgaagcaatg attacaggaa tatgcatggt tgttgcattg attggaccga tagggataaa
     8161 acctaaaggc gtaacagcta ataaaatcaa aatagctaaa aacatcgcag ttaatgtaaa
     8221 gttttttgta tttttcataa gtactccttt tttctaagca tttctttcat taagagtcag
     8281 catgacctgt tcgatgatcg tctgaatatc agctaaggca ccttttccaa aatctccaca
     8341 agctaaaaga gattctcttg gttcaatttc ctgatagccg acagatctca atgtttccag
     8401 attacgctga gtgatcgggt tttgatacat attcgtgttc atggcagggg caatcaattt
     8461 tggtgttccc actggaagcg ccattgcgac tgttgaaaga agatcgtctg caatgccgtt
     8521 tgctagcttg ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt cagcttgttt
     8581 tgctagttcg atatgattga ttacctgcgg atttgattct tgcatgacgt ctgtatggac
     8641 tgcgcgcttc gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc tgcttttaga
     8701 cataatcaca tctacctggg cacctaattt gacgaattga cttgtaagat cagcgctttt
     8761 gtaagcagaa atacttccag taacgcctag taagatcttt ttcattttga ttcctccagt
     8821 ctaattttct cagtaatcaa tgcagcaatc tcagatttac tttgagcttc ttccactgtt
     8881 ccatctttag ataagagata gccgtggtgt gtctcgccat ggatttccgt caaatcattt
     8941 gcgaaaataa aatcagtttt gttatttatc agactatttt ttgcgactgc cagaagagat
     9001 tcctttggta catctactaa tagtttgaaa ccaatgattt ttgcttttgg ctgccattca
     9061 cgtagataag aaatgatttt tggtgttttc ttcaaaacga gcacaagatg ctcggtgtta
     9121 gaagagatct ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca attgtccagt
     9181 tcttcttgcg ttgaagaagt gggtaggttt tttgccagct gttcttctga aaaactaaaa
     9241 gcaggagtaa aatcactgac tgccatactg tggataatcg catcgtagtg ttcttttttc
     9301 gttagttctt ctaattgtaa aaataagtcc agtgcagatt cgatcgtata aatcgttata
     9361 ttggagtgtc ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat tgtggtatcc
     9421 ggattggcag ctaaataatc agctaatgct tgccctaatc gtcctgtcga atgatttgta
     9481 atagcacgta cttgatcgat tttttcagtc gttcctccag ctgtgattag aatcttcatt
     9541 tatggtcacc tacatttact agtgttcttc cttggtgtgt tccagcaagt agtttagatg
     9601 ctgtttcagg caattgatcc agagtaatct cttggacgac cagttcatct gcaatctgca
     9661 aatccgccat ctgttgccat aaagaaagac gttgatctat tggtacattg acagaatcaa
     9721 ttccaatcaa ctggatgttt cgcagaataa aaggcaagac agtcgcgttt agtttgattc
     9781 ccccagcatt gccacataaa gctactgcac cgttataaga gatcaatgga agaagactgc
     9841 ttagctgctc gccacctact gtgtcgatga catagtcgat ttgctgtttt cctaaagctt
     9901 ttgttgtctc tggcagaaat tcatcaggtc gtacgacttg caaagcacct agacttttta
     9961 gccatgtgac agactcttct tttctggaaa aggcaatgat tgactggaat cctagtttat
    10021 gtaacagggc tatcgctgta ctgcctacac cgccagaagc accagtaacg acaacttttt
    10081 tatcggaggt cataccttga cggattaaag cgttcacaca caacatagca gtaaaccctg
    10141 ctgtaccaag gatcatcgct tgacgcagcg tcatattttt tggtagagga actaaccagt
    10201 ctcctggtac tttttgatat tggctataac cgcctggatg ggtgacgcca agcccaaaac
    10261 cagtagcaag tacaagatct tcttttgaaa aacgtggatt gttactttgg acgacagttc
    10321 cggataaatc aatccctgga gtcatcggat atgttcgtat cacctgacca gactcgctca
    10381 ttgccaatgc atctttataa ttgacatcag aataggcaat ttttaccaag acttcatttt
    10441 cagccaactc agtaaatggc agatcactga ttgctgtatg gaaaacaggt tcctttatta
    10501 cctgaaaaga tcgaaaagcg ttcattcgtg agacactcct tttaaaattt tcttttatca
    10561 taccataaag cctactcttt tattcttgtt cttccgtaaa tttagacaaa aattaggtga
    10621 aaaataaaga atcaaacaac tagaatttat tccaaaagat aaagaaatat tcagcaaaaa
    10681 aaattttaca aagggttgcc tttttcaata aatttcgtat actaatacga gtggtagtaa
    10741 acaggaagtt tactattaga aaacttcgat tcggaggaaa ttatggaaat tggtgagaag
    10801 ttaagaaatt tgcgcatcca aaagaatttg acacaagaag aactaggcga acgcacagat
    10861 ttgtcaaaag ggtatatttc ccagttggaa cgagatttaa gttcaccatc aatggaaaca
    10921 ttcttttcta ttcttgaagt cttaggtgtg actccagaag aattttttca tcaagaagca
    10981 gcgaatctgc aagtggttta ttcaaaagaa gatcatacgg tttatttcga tgaagaaaac
    11041 gggtatgaat tggaatggct cgtggctgat tcgaatgaaa aagaaatgga gcctgtcttg
    11101 ctgacttttg aaggtgcagg agaatataag acgtttgaac cttcattatc tgaaacgttt
    11161 atttttgtat tagaagggga attagagtta agcttaggag agacggtcta tgtggcaaaa
    11221 gcaggacagt cgatctatta tcaagcgaca aaacaacatc aattaaggaa tcatctcaaa
    11281 aaacgaacaa aagtattgat tgttgcaacg caatcatatt tgtaatatag ggggtaaaaa
    11341 cgtggggaaa ccaatcattt catttgatca agtagtgaaa cgctatgatg atgaaacagt
    11401 attaaaaaaa gtaagttttg agatcgaaca aggaaaattt tatacactgc ttggtccttc
    11461 cggctgtggt aaaacaacga tcctacgtat tatagctgga ttcaccgaag ctacagaagg
    11521 ggacatctat tttgaaggga aacggttgaa tgatttgcca gctaataagc gacaagtaaa
    11581 tacagtcttc caagattatg cactttttcc tcatatgaat gtttttgata acgttgcttt
    11641 tggattgaag atcaagaaaa tgtccaaatc agatatcgaa aagaaggtaa aagatgcttt
    11701 gcgtatggtc cagcttcctg gatacgaaaa acgggagatt agtgaaatgt ctggtggtca
    11761 aagacagcgt gtagcgattg cacgagcaat tgtaaatgaa ccgaaagtat tgctattaga
    11821 tgaaccgtta tcggcgcttg atttgaaact acgtacagat atgcagtatg aactgcggga
    11881 tctgcaacaa cgattaggaa tcacgtttat ttttgttact catgaccaag aagaagcgtt
    11941 ggcaatgagt gatgagattt ttgtcatgaa taaagggaaa atcgtccaaa gtggtacacc
    12001 cgtagatatt tatgatgagc caatcaatca tttcgttgct gattttgttg gggagagcaa
    12061 tatcgtggac ggtgtaatga tcgaagataa tctagttgag tttgttggta agcaatttga
    12121 atgtgtcgat ggcgggatgc gtctaaatga accagttgaa gttgttttac gcccagagga
    12181 tttgacgatc acgacaccag ataaaggaaa actagtcgta acggtcgata ctcagctgtt
    12241 tcgtggtgtc cattatgaaa tcatctgtta tgatgaacag caaaatgaat ggatggtcca
    12301 ttcaacgaaa aaagctaaag aaggaagcaa agtaggattg gcctttgagc cagaagatat
    12361 ccatgtcatg cgtttcaacg agtctgaaga agaattcgat gcacgtcttg acagctacga
    12421 agaatagggg ggacaagaat gaaaacaatg cgcaggatct attccgtccc ttatattttg
    12481 tggctcggtt tattcgtgat cgcgcctgta ttaatgatca tttatcaatc tttttttgat
    12541 atgaatgggc aattcacgct gaataattat ctatcttatt ttacgtcggg gacatcgctt
    12601 tctatgacga tcaattctgt atggtatgca tttttgatca cgtttttcac gctgatcatc
    12661 agttacccga ctgcttattt tttaacaaaa ctgaagcaca agcaattatg gctgatgctg
    12721 attattttgc ctacatgggt gaatctgctg cttaaagctt acgcatttat tgggattttc
    12781 agtgtgcacg gcagcgtgaa tcatttcttt gattttatcg ggatcgggcc gaaacagata
    12841 ttattcacag acttcagttt tctgttcgtg gcaacgtata tcgagattcc atttatgatt
    12901 atgccaattt tcaatgcatt ggaagagatg aatccttctt tgatcagtgc tagtagagat
    12961 ttaggcgcga atagctttga gaccttccga cgagtgattt ttccgttatc attaaatggt
    13021 gtgaaaagtg gtgtccaagc agtatttatt ccttctcttt ctttattcat gctgactcgt
    13081 ctgattggcg gaaatcgggt gatcacttta ggaacggcta tcgaacagca tttcttggtt
    13141 acacaaaact gggggatggg ttctacaatc ggtgtgattt taatcattgc tatgttcatc
    13201 gtgatgctcc tgacaggcga gaagaaaaaa ggaggtcgct cataaatgaa aaaatttaaa
    13261 tggtcgtatc tttatttagg gatcgtcttt ttattcttat atgcaccgat cttctatctg
    13321 attttttatt ccttcaatga aggaggaacg atgaacagct ttacaggatt tacttgggaa
    13381 aattatgaag ctgtttttga agatacacgt ttgatcatta tcgtattgaa tacgttcatg
    13441 ctggctttct tatctgcatt gatcgccacg atcatcggga catttggggc tatgggaatc
    13501 tattacacga aaaaacgacg tgcacggaca gcgttgttga gcatgaataa catcttatta
    13561 gtctctcctg atgttattat cggtgctagt ttcttgattt tctttacact actaggattc
    13621 ggattaggtt ttcagagtgt attgctttcc catattgctt tcagcattcc aatcgtcgtc
    13681 ttgatggtct tgccaaaatt gcaggaaatg aacgattcaa tggtcgatgc ggctagagac
    13741 cttggtgcta ataatttcca agtagtcaaa aatatcattt tgccttatct ttctccagga
    13801 atcatagctg gatattttat ggcatttacc tattctcttg atgattttgc ggttacattt
    13861 ttcgtcacag gaaatggctt tacgacgctc tcagtagaga tctattctcg tgcaagacaa
    13921 ggaatcagtt tagaaatcaa tgcgctaagt gcgttagttt tcttgttctc catgatattg
    13981 gtcattggat actactttat tagtaaagaa aaccgtccaa aacggctgaa gaaaaacaga
    14041 agagtacgcg agggggtggc acggctgcga tgaaaaaatt acaatctctg tttttaggta
    14101 ttctcatcat tattgcggtg ctgctgcttg gtgttcgtca acttgaaaaa acaagtggca
    14161 tgagtggagc aaaggtactt accatctata actggggaga ttatatcgat cctagtttga
    14221 tcacgaaatt tgaaaaagaa tatggataca aagtgaatta tgaaacattt gattctaatg
    14281 aagcgatgtt cacaaaaatc cagcaaggtg ggacaaacta cgacatcgct ataccaagtg
    14341 aatatatgat ccaaaaaatg atcaaagaaa aactagtact tccattagat cattcgaaga
    14401 tcaaaggatt aaataatatt gatccacgat ttttagactt ggattttgat ccagataatc
    14461 agtattcgat tccatatttt tggggaactt tagggatcgt atataacgat aaattctttg
    14521 atgcaaatca aattaagcat tgggatgatt tgtggaagcc ggaattaaaa gacagtttga
    14581 tgctgattga cggtgcacga gaagttatgg ggctttcttt gaatagttta ggctattctt
    14641 tgaatagtaa gaatatggca cagttgacag aagcagcaaa taaattgaat catttaacac
    14701 caaatgtcaa agcaatcgtg gcagatgaga tcaaaatgta tatgatcaat gaagaagcct
    14761 ccgtggcagt gacattttct ggcgaagctg ccgatatgat gtgggaaaat gaacatttac
    14821 attatgtgat cccaccagaa ggatccaatc tttggtttga taatatcgtt atgccaaaaa
    14881 ccgtgaaaaa taaagaaggt gcctatgatt tcatcaactt tatgctagaa ccggaaaatg
    14941 cggcacaaaa tgcagagtat attggttatt ctacaccaaa tgaagaagca aaagcgttat
    15001 tgccaaaaga catttcgtcg gacgaacaat tctatccatc tgatgacacg atctcccatc
    15061 ttgaagtcta caaagatcta ggatcgaaat acctaggcat ctataatgac ctattcttag
    15121 aattcaagat gtataggaga taatggcaaa actcagtaag aatggggact tatcaacgtt
    15181 ctaaagagct aaaaatcgtc ctttttctat ctgagttttc caatcttcta ccaaaagcgg
    15241 atgaaacgcc gctttttttg tgttatttta ttttttctac cacgtttttc taccagagaa
    15301 aagtggacaa aaccattgta aaatagggcc cgagacgggc tagtaaatct aagattgaaa
    15361 aagttacaga aggataaagt aattggaaac tatgcaaata tacttaaccc ttctgtaatc
    15421 tattaataaa actaatatat gaatccagtt caatatttgt agctatgaaa aaacataaaa
    15481 atcatattag ataaaaacta aattggtaga taaattattt tgtcagatat ctgaggcaaa
    15541 tattcgacta tttgtgtttc aaagtaaaaa ttggaataca aatatagaat agacatattg
    15601 cagccgatag tttattatgc ttttagacac attaaactgg aggaaaaaat gaaaaagagc
    15661 gctaaagatc gagtttttga tgagctgata cgactaacag ggaatatgtc ttctgtcact
    15721 gccgagaaaa tttcgaagaa tctaggtatc tctcgtcaga atgccagcca ttatttgacg
    15781 agattagtgg aagataaaaa ggtcgagaaa ttaccgggaa agcctgtttt gtggaagcct
    15841 ttagatgaat atgcggttgt agataatacg gagcaaataa acgaagcctt tcattctgta
    15901 gttggtcatg acggaagtct gagagaggtc attcaaaaat gtatagccgc tgtcaaatat
    15961 ccgcctaacg gactcagtgt attaataaat ggagccacgg gtgttggaaa aagtttttta
    16021 gccacaaaaa tttttgaata cgctatccat gagcaaatta ttgaaaaaga tgcaccattt
    16081 gctatcttaa actgtgccga ttatgcggac aatcccgaac tgctttcggc aacactgttt
    16141 ggatacaaga agggggcctt tacgggggct gaaaaggata ccgatggttt attggcaacg
    16201 gccaacaacg gctatctttt tctcgatgag gtccatcgcc tatcgaaaga aaatcaagaa
    16261 aagctctttc tatttattga tactggaaac tatcgtccag tcggtgaaaa tgtaaattgg
    16321 cattctgcta aggttcgttt tattttcgcc acgacggaaa aaggtgaaaa ttatttatta
    16381 gacacctttg atcggcgtat tcaaattagt gttatgttgc caacttttga agatcgcccc
    16441 attcgagaac gattagagct gattcaatta ttttttcaaa atgaagcgga tgttttacaa
    16501 aaagatatta ttgtttcaag agaagccttg tctttaatgt tatctcatcc ctttagtggg
    16561 aatattggta agctgaagaa tatcattaaa atcagttgtg cggatgtgta tagtcgaaca
    16621 caagaagaac cattaacgat tgggaaaaat gagatcttga ttcagttaaa tatgctggaa
    16681 tccgaagtag aaagcttgcc gattgctagt ttattgataa agaaaaatca aaagataaaa
    16741 acggatcgaa tcaaaccgtt tgattatgat tcgattttgg aaaaaattac tcagctgata
    16801 aatgaaggta atttcaatga aaatttttct tcgataaaga atgaagtaca gcatttggtg
    16861 catcacattg ataaggattc ttttttatca atgagtagaa aattgatgat tcagatgttt
    16921 agaaaggtct gggtatccgt tttaggaaaa acgtacggat tgacgacgag tggacctgtt
    16981 gcagaaatta ttagcagatc ctatgtcatt tccccaagct ataagacaga cttaaccggg
    17041 ataaatcgta cattagctgc aaatctacct cggacgactt acttatgtaa tcagttcttg
    17101 gagcatttac caccgctctc agaaaaggac gagaactttc taagactgct tattgtcgtg
    17161 actttatctg attatgtaga tgaatccatc gagctgaagg gcctgttgct tgctcacgga
    17221 gatagtacgg ctagcagcat tcaagcagta gtgaatcaat tatgcggcaa ttatgttttt
    17281 gaagcgttgg atatgccagt agagagtaat gtgtcagaga ttgtcgaatc gacaaggaat
    17341 ttcgtgaaac ggcaagtgaa tacagatagt ctagtgctga tcgtagatat gggctcttta
    17401 aatcaaattt acacacagat caagaacgat ttaaaagggg agttacttct tctgaataat
    17461 ttgacgactt ccattgcact tgatattggc ttgaagatga cgatgaatgc gccatttaaa
    17521 cagattgcgg aggatgcagc agacaagtat tcaatcaatg ttcaatactt tgaaggcttt
    17581 tctcaaagta gcaacattat catttcctgt atgtcgggtt tggggatttc ggacaaattg
    17641 aaagatgttt ttcgtcatca tttatcggat gaatccatcg aagttttcac gaaggactac
    17701 agagaactac gtgaattaat cgaccaaaat gatgaaactt attttgacaa gacaaaattg
    17761 gtcatcacga cttcggatct tccacgttcc ttttcaattc ctaatattaa tgtttatgac
    17821 atcttagacg gtgatggagc agccaatttg gagcatattt tgagtcctga aatgaagccg
    17881 gcagaattta accgcttgat tcaggagcta gtaaaattct tttcgttgga aggaattgcg
    17941 gaccgtttga actttctgaa cccaacgatc gtgatgagtg aagtggaaac agtcatttct
    18001 aaatatgaaa attactatca tttcacttta aatggccgtg taaaactaaa tctctatatg
    18061 catacggcat tgatgatgga acgtttattc ttggctcgga cagaaaaatc tgaagaaccg
    18121 aattatgatt tgaaaccaga tgaagatgaa ttttattatg tttctagaag tatttttcaa
    18181 tcaatggaga tgaaatataa ttttaaagtc aatgcttatg agctttcgct gctctatgaa
    18241 ttattaggac cgtatattca aaaataaagt gtctaaaaaa gtgtctaatc tgtttttttt
    18301 aggttagaca ctttttttaa tgtgaattaa cgtgattaaa ccaatgtttt taaaagttgg
    18361 cacaggaatt gcttatataa aagtgaacag aaagaaagga attaaccata tgaagaaaat
    18421 tctaatagcg actcacggtc acttagcttc aggctttctt agcagtattc aattactgac
    18481 ggggaaggtt caagagatta cggtgattaa tgcttatgtg gatgagtgtg attttgaaca
    18541 ggagttgaat gtatttgtaa accaggtgaa cgagcaggat cagatttttg tttttaccga
    18601 tttatttggc ggaagtgtca atcagaagat taccaaaacc tttttggaga aagagattgc
    18661 tgtaactatt ttgtctggct ttaatctgcc aattctgctc gaaatagtat tggcaaccaa
    18721 ggaattaaca aaggaggata ttcaggaact ggtaaataaa tgccaacagg aattaaagat
    18781 gaccattctt gatgaggtac atgtagtaaa tgaagaggaa gatttttttt aggaggaaga
    18841 aaaatgatta aagcagtgcg aattgatgaa cgattaattc atggacaagt agcgatgact
    18901 tggacaaaga gtctaaattt aaccgggtta gtagtagcaa gcgatgaggc ggctaccaac
    18961 gatattcaaa agatgacatt gaagatggct gcaccatcga atgtcaaagt cattattaaa
    19021 acagttgatg acgcgattca tttgttaaaa gatccgcggg cgaaggacat gcgcttgatg
    19081 gttttagtcc ctaatgtcaa agatgcggta aaagttgcga aagctttccc aacggaaatc
    19141 gaattaatga acgtggggaa tgctgggaaa atgacaccgc catcggaaga taagaaaacc
    19201 ttatcaaagg aagtgatgtt aacgacgtca gaaattcaag acttaaaaga attagttgaa
    19261 ctttatccag ataccgtttt tcagcctaca ccggcgatgg aaaaacgttc agctatctct
    19321 attttgagtg atttttagaa aaaggaagga gaaaaattat gtatgatgca tttgtagtcg
    19381 ctttggccgt ttttattgga attgctggac atgaaatgtt cgggatgtcc atgctgagtc
    19441 gtccgattgt tgttgctccg ttagcaggag cgttgatggg ggatttgcag acggggttga
    19501 ttgtcggggc gtcactagaa gccatcttta tgggtgtcgt caatatcgga atctctagta
    19561 cggcggagcc ttctttggct gctgggctgg ctacagcgtt cgcaatcaaa atgggaggaa
    19621 acgtggatgc gattattccc attgtctttc cactagcagt attaggatta cagttaatga
    19681 atatgatttt ttcttttgta tgtggaccga tggctgctaa atttttcaaa tttgcaaaat
    19741 ctggtgatca aaaaggaatc atcaatctgc acttctttat gtggtttgtc cactatgcgc
    19801 tttatgctct tattccattc tttgccgttt tatttggagc agatgttgtc aaaagcgtct
    19861 tggataatat tccagaggtc atcatgaacg gattaacagt cgctggaaat ctgctgccgg
    19921 cagttgggat ggcgatgctg ttacggatgc tttggagtaa aaatattgct gtgtggtttt
    19981 tctttggggc cgttgttatg gcgtatctcg agttaccatt gattgcaatt gccgcagttg
    20041 gaacgattat tgcaattgtt atagcccaaa gagatctaca actaaaaaat atatctgttc
    20101 atgcagttca aagcgatgct gctgtaaatg caacgcttga ccaagaagag gaggatttct
    20161 ttcaatgaaa ttagctgatg gaatttctaa agatgagaaa aaattggttc ggaagatgtt
    20221 ttggcgctcg atgactatgt atgcttcggt gaatccggtg acgatgggcg gcggtggttt
    20281 cgcctattct atgatgccct tcattaatca tttctacaaa agtgaagaag atcgagctgc
    20341 ggcgctggaa cgtcatacac agtatttttc gacgaccatt ccttgcgcgt cctttgtcat
    20401 ggggattgcg gcatcgatgg agaaggaaaa ttctgaaaaa gctggttttg acgtggaaag
    20461 tatcaacgcg atcaaaacga gtttgatggg accattggct ggaattggcg attcattgtt
    20521 ctggggagtt tggcgtgtcg ttaccgcggg actagcgatc aacttagcca atgccggaaa
    20581 tattttggca ccaattatct ttttagttat gttcaatata cctaattact tgatgcgata
    20641 ctatggcggc gttttaggct attcattagg ctctaaatat attgaaaagc tgtatgcgaa
    20701 cgggttaatt gaaattctga caaaagctgc tagtatcttg ggtctgatca tggtaggtgc
    20761 aatgacgatc agtatggtca aattcaatac cgttttgagc ttttcaatga agggcgaaga
    20821 actaatgaat gtccaagaaa tcctcgatag tatttttaaa ggaattgtac cgttattggt
    20881 aacattgagc tgtttcaaat tactagataa aaaagtcggc gtcatcaaaa ttatttttgg
    20941 aatggtcgtc ttgggaattt tattgtcatt acttggaatc gcatagtagg aagctgcagc
    21001 tactaggctg cagttttttt agcaaggagg gaaaagatga ctattaatga agtaatttat
    21061 tcacccaaac acccattttt taatgctgag ttaaacgacg gaaatgtgac tacttcaaac
    21121 gaatacagct actatcggaa tattcctgat gtaaagggat tgaccttcga tgtaagtaat
    21181 caaaagacat tagcaaacct tgatgtttgc gggacgattc aaacgttgac cttttatcga
    21241 gaaaatttac taactgagga aaaaccaggt gtttgggtca ataaacagct ctctcaaacc
    21301 aattcgttat ccttaaaggt tgaagtaaac gggattgtct accatttgtc agaatcggat
    21361 catcgtattg aagtcgatgt gattcaagac tgtttgccta gatatattca tcattattcg
    21421 acgtttaagg tcattgtagt atcctttgct cccatactgg ctaataagcg tttaagtatg
    21481 cttgttcaac aggtttacat tgtgaatgaa acacacgaaa gccttcaagt ttcagtgaaa
    21541 gatgtgcctt cgtatcaaga gaagtattcg gatcagcaaa atgtgttgat ttctcaaaaa
    21601 ggaaataagg acacgatcgc acaacaggaa gtggcaagct tcgccattgc ttttgtggac
    21661 ccaaatgctt ttgaagaagt aatgactttt caggaatctg atatcgaaaa atggctgaag
    21721 gaaacattga actattttgc tcagctattt gggcagctgt caatgccaaa cgatagagtt
    21781 gtccatctct atcgtcgtgc actttatcaa agcttttcta gtttcggtat gaatcgacag
    21841 ggacaggtcg tcggcagcaa ttggggcagc tatcctgcta ctaatcgaat ttggaataag
    21901 gatatgtatt acgcatcatt gccattcacc atgtttgaac cggagttatg tcaaaaaacg
    21961 gttctatggt ttgatcaata tggcgttaag tttcctggtt caaaatttac aggaggtatc
    22021 aatcattctc tgagtaattc gttatccagt gcgttactgg ctagtcttta ttttgaacat
    22081 actggggata caaaattctt tgagcggtgt ccgcaggttc tactgaatgc ttcaagaatt
    22141 attgaggaca tattagctca acgtcggtca ggggaaccaa tgctttttcc atctgtttgg
    22201 ttatcagacg cctttgcact tggaaagtat cataccggca gcaatctttg tctttggaag
    22261 gcctgtattg gacttgcaga aatttttgaa gccctagaac aactgagtct tagcaagcga
    22321 tacaaaaaca ttgcctgtaa actaaaggaa tcaatttgta aacaaatgac tatcgatggt
    22381 ccttttggtg agcaattttt ggagggaatc ggtgatgagg agaaaacaac ctattcagtg
    22441 aagcactatc aaaaaccgat tcttgagcaa gggttaatct ttttaagtga tgtgattgtg
    22501 gatggaaaaa ttaatttatt gatgcatgat ggagaagaat cagacactac gttgatgcct
    22561 ttttatcaat ttttagataa taaagatcat ttatatcaga acaccatggc cttttctgct
    22621 agttctttca atccaaccta tagcgaggaa atcaaaggca tcacttgggg attagaatcc
    22681 ggcgccactt ttcctggctt tatcactgtt ttaatgagcg agctgcacaa ccctcaagct
    22741 ttcgtaaccc gattagaaga actaatttgt ttggcagatt tagacggttc gtggtggtgg
    22801 tggccgtatc gcttagaggc tcaacaagga gaagtcgtgc gagactttgg ctgcggcaaa
    22861 tgcggctggg catcgggtct ttttgtcagc ctaatgatca gtcaatattt cggattgaaa
    22921 ttcaaaaaag gagtattgca aattgatcct gttgataatt taacgttttc ttggaagaat
    22981 ttgaagttcg gtcaagcctt catttcgatt gaatgtacgc aatccgagtt gagtatttca
    23041 aatttgtcag aaaagccatt gaaaataagt gatacgaaaa aaattcttca cgtagaaaag
    23101 ggaaaaacga ttcacattca aaggagaaaa agatgagtat actaaaaaat attacccaat
    23161 ttaaaaaaat ccagagtcga gcaatcactc ccgaaaatcc aacaggcgag aaaggtaaag
    23221 ctgcgatgtt gccaagcgag ctaggaccaa gccgcaaagg tcgtggcgca attccgctga
    23281 cttctggtga agaaactgtg attgcggata ttactggaac gggaataatt cgtcatatgt
    23341 ggatgacgat tcgtgatcga acagaatttg gcagttttgt tttacgtgat ttaatcttaa
    23401 gaatttattg ggatgggtcg gatagccctg ctgttgaaag tcctttaggg gatttctttt
    23461 gtaatgggtt tggagagcgt tgcgaggtga actcgttgcc gatagtggtt aatcctacag
    23521 ggggctttaa ttcctatttc gaaatgcctt tccaaacatc cgctaaaatt acgatcgaga
    23581 atttacatcc gaaggatatg tcctcatttt tttatacgat aaattacgct ttggtggacg
    23641 aattggaaga aaatagccta tattttcacg caaattggca tcgggaacgc attaccaaat
    23701 tacaggagga ttataagtta attgatcgat taaagggtca aggctattac gtgggtactt
    23761 accttgcgct tacctgccta gaacgttatt ggtggggcga aggcgagttt aaattctaca
    23821 ttgatgggga tagtgagtat ccgaccgtaa cctcaacagg ttcagaagat tattttggcg
    23881 gggcgtgggc ctttcatgag tacgatgagt tcggacgtcc acatgcgaaa aactattcaa
    23941 cagccttttt agggtatcct taccaatcaa atagagatgc cacaagagat cgttttgaaa
    24001 cgggaaaatt gaatgccgtt catgcctttg gtgacgacgc aatgccacga catggtcttt
    24061 atcgttggca tcttcaagac cccatctgtt ttaccgaaga tttatcggtc actttacaac
    24121 aaatcggcaa cgatgatgtt cgcttatttg agcggcaaga cgatgttgct agtgtcgctt
    24181 actggtattc aacagaagct tcaggaaata ctaaacgtca tttcactgat cgagaacgct
    24241 tgccgcgtta atgaagtgtt tttcacatat tcgaagggag gaacctatga tgatatatgg
    24301 ggcaattgaa gctggaggga ctaaatttgt ctgtgcagtc ggtactgagg aattaacgat
    24361 tttggaaaga gtcagttttc ccacaacaac tccagttgaa acaatgaagc aggtcattga
    24421 tttttttaaa ccttttaagg aacaattggc aggcatcggg gtcggctgct ttggtccaat
    24481 tgatattcaa tgcaaatcaa cgagtttcgg tcatatcaca tcaacaccaa aacttgcttg
    24541 gcaaaatttc aattttgtag gaacgctaaa gcaatatttc aatattccga ttgaatggac
    24601 aacagatgtc aatgcggctt gctatggtga atatgttgct ggttctggca aagggttagc
    24661 aggtgttgca tacttcactg tgggaacagg tatcggaggt ggtgccttgt tcaatgggac
    24721 tttcgtggaa gggttcagtc atccggaaat ggggcatatg ttgatcaagc gacatccaaa
    24781 ggacaatttt tcaggaaact gtcccttcca ccaagattgt ttagagggga tggcagcagg
    24841 accagcgatt gaaaaacggt tgggtgtaaa aggccagaat ttattggctg acgattcgtt
    24901 ttggcagatt gaagcctttt accttgccca atgtgcatac aatacaacac tgatgttttc
    24961 gccagataga attatttttg gcggtggtgt aatgaaacaa gaacacatga agaaaaaagt
    25021 tcaggataaa tttgttgagc ttataaatgg atatgttgaa ataccaccaa ttgatagcta
    25081 cattattacg cccgaattag gtgataatgc cggaataatc ggcggattag cattggccag
    25141 aaaagcggta agaaataagc aaccataatt aatgaatgtt tcattgatac tcatgtcaaa
    25201 agtatttaac cgagtaatca aatgatcaaa tgaagttgga taaagcgaaa atgaattcaa
    25261 ataaaaaggc tctaagtcaa atcgtgttgt tttcattaaa tcactgaaag gaaatgcttc
    25321 tgcgtttcct tttttctttt tgttgtgcgc tgtatacctt gaggatattc actggtggcg
    25381 tacacttaaa tagtaatata ttgtgttact ttatgtctgt ggtaatagtt atagttgctt
    25441 accaatgtat gttcattttt cga
//
LOCUS       DAZKBH010000027        24897 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000027 DAZKBH010000000
VERSION     DAZKBH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.027"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..972
                     /locus_tag="ACG8OO_001124"
     CDS             115..972
                     /locus_tag="ACG8OO_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9045015.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1087..2007
                     /gene="pstC"
                     /locus_tag="ACG8OO_001125"
     CDS             1087..2007
                     /gene="pstC"
                     /locus_tag="ACG8OO_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFP9045016.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2007..2891
                     /gene="pstA"
                     /locus_tag="ACG8OO_001126"
     CDS             2007..2891
                     /gene="pstA"
                     /locus_tag="ACG8OO_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9045017.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2906..3712
                     /gene="pstB"
                     /locus_tag="ACG8OO_001127"
     CDS             2906..3712
                     /gene="pstB"
                     /locus_tag="ACG8OO_001127"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9045018.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3728..4486
                     /gene="pstB"
                     /locus_tag="ACG8OO_001128"
     CDS             3728..4486
                     /gene="pstB"
                     /locus_tag="ACG8OO_001128"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9045019.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4499..5176
                     /gene="phoU"
                     /locus_tag="ACG8OO_001129"
     CDS             4499..5176
                     /gene="phoU"
                     /locus_tag="ACG8OO_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9045020.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5753..6445
                     /locus_tag="ACG8OO_001130"
     CDS             5753..6445
                     /locus_tag="ACG8OO_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9045021.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6442..7209
                     /locus_tag="ACG8OO_001131"
     CDS             6442..7209
                     /locus_tag="ACG8OO_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9045022.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7309..8877
                     /gene="liaX"
                     /locus_tag="ACG8OO_001132"
     CDS             7309..8877
                     /gene="liaX"
                     /locus_tag="ACG8OO_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFP9045023.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9174..9494
                     /locus_tag="ACG8OO_001133"
     CDS             9174..9494
                     /locus_tag="ACG8OO_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFP9045024.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9494..9844
                     /locus_tag="ACG8OO_001134"
     CDS             9494..9844
                     /locus_tag="ACG8OO_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9045025.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10011..10949
                     /gene="hprK"
                     /locus_tag="ACG8OO_001135"
     CDS             10011..10949
                     /gene="hprK"
                     /locus_tag="ACG8OO_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFP9045026.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10964..11794
                     /gene="lgt"
                     /locus_tag="ACG8OO_001136"
     CDS             10964..11794
                     /gene="lgt"
                     /locus_tag="ACG8OO_001136"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9045027.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11838..12863
                     /locus_tag="ACG8OO_001137"
     CDS             11838..12863
                     /locus_tag="ACG8OO_001137"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9045028.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12878..13816
                     /gene="galU"
                     /locus_tag="ACG8OO_001138"
     CDS             12878..13816
                     /gene="galU"
                     /locus_tag="ACG8OO_001138"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9045029.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13941..14381
                     /locus_tag="ACG8OO_001139"
     CDS             13941..14381
                     /locus_tag="ACG8OO_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFP9045030.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14384..14986
                     /locus_tag="ACG8OO_001140"
     CDS             14384..14986
                     /locus_tag="ACG8OO_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9045031.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15045..16151)
                     /locus_tag="ACG8OO_001141"
     CDS             complement(15045..16151)
                     /locus_tag="ACG8OO_001141"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFP9045032.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16361..17362
                     /gene="ccpA"
                     /locus_tag="ACG8OO_001142"
     CDS             16361..17362
                     /gene="ccpA"
                     /locus_tag="ACG8OO_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFP9045033.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17699..20044)
                     /locus_tag="ACG8OO_001143"
     CDS             complement(17699..20044)
                     /locus_tag="ACG8OO_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFP9045034.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20323..21225
                     /locus_tag="ACG8OO_001144"
     CDS             20323..21225
                     /locus_tag="ACG8OO_001144"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9045035.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            21438..23213
                     /locus_tag="ACG8OO_001145"
     CDS             21438..23213
                     /locus_tag="ACG8OO_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFP9045036.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23279..24865)
                     /locus_tag="ACG8OO_001146"
     CDS             complement(23279..24865)
                     /locus_tag="ACG8OO_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9045037.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
BASE COUNT         7960 a         4467 c         5205 g         7265 t
ORIGIN      
        1 tagttttttt tacaattatg agaatttatt ttgttattta atgattttga ttcgttttca
       61 tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt tctaatgaaa
      121 aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg ctgttcaaag
      181 tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca gcctttagta
      241 gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa tgtacaaggg
      301 ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat cggaaactct
      361 gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga tcatcgagta
      421 gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga catcagtatg
      481 gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt cggtggaaaa
      541 gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag cacgtttgaa
      601 aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc aagcggaatg
      661 gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt ttcttatgtt
      721 tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga aaatgtgaca
      781 acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca tccaaaaaaa
      841 ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa catcgttgga
      901 gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca aggaaatatc
      961 gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac atgttattaa
     1021 aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat taagggggag
     1081 tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc aaaaatggaa
     1141 cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt tgtattatct
     1201 attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa gatcaatgta
     1261 tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa tgggaaacca
     1321 atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt atctgctatc
     1381 gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc tcctaaaaac
     1441 ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc tgtcgtttat
     1501 ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg cgggactggg
     1561 tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac tgtcacaacg
     1621 atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc attagcgctt
     1681 ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt gcccgggatt
     1741 ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc tatccaaatg
     1801 gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc tacattgacg
     1861 tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa taatgtgtta
     1921 tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt gatccgtatg
     1981 attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa attgctacag
     2041 gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta ctactttata
     2101 tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct tccaaagcct
     2161 accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg cttttgatca
     2221 cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca gaatatgctg
     2281 gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc tcgttgcctt
     2341 ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag tatggtttct
     2401 cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg actagaacag
     2461 ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg gccttaggtt
     2521 tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca gggattttga
     2581 ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg atctatacgg
     2641 ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt gtttcaagcc
     2701 cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag atcaatacag
     2761 aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg ttgattattg
     2821 ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac cgaaaaatga
     2881 cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa caaatgtcat
     2941 caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg tctggtatgg
     3001 cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga tcactgcact
     3061 gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc gaatgaatga
     3121 tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag atattaatgc
     3181 gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc aacgtcctaa
     3241 tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc acggcgaaaa
     3301 aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg ccttgtggga
     3361 ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc aacagcaacg
     3421 tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag acgagccggc
     3481 aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa acttgaaaga
     3541 agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta tcagcgacta
     3601 tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca agatatttac
     3661 cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt aagacggagg
     3721 cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt atggaaagaa
     3781 agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg cgatgatcgg
     3841 tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga atgacctgat
     3901 tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct atggtccaaa
     3961 aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc caaacccatt
     4021 tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg aaaaagataa
     4081 aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt gggatgatgt
     4141 gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc agcgtgtatg
     4201 tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac caacaagtgc
     4261 gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa gagagcggta
     4321 tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag ataagacggc
     4381 attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat tcatgaatcc
     4441 agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag gaaacggaat
     4501 gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct acgaaatggg
     4561 gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac acgataaaat
     4621 cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa cacgcttgga
     4681 gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt tgcgtatgat
     4741 cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg cggtgtctgt
     4801 tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg aaaaagagat
     4861 ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg cttatgtcaa
     4921 aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca atgactattt
     4981 caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga cagtcatcag
     5041 tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact atgtgacaaa
     5101 catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa atacgaacca
     5161 caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa agaggttgtg
     5221 aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc aaacaaaaaa
     5281 tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc ttttgaacac
     5341 cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca tcatttcata
     5401 gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta tttgtgtgct
     5461 tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca tcgagataga
     5521 aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga acacggatca
     5581 aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag aaacagcaaa
     5641 tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc agctagtagc
     5701 aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg ccatgttgct
     5761 tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata tcgttttact
     5821 tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa atatatctat
     5881 cctgttcgtc gttataggga tgattattag catattggct ttagatggat ggaatagaag
     5941 cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt ttatttgtct
     6001 tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga attatctgac
     6061 acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg gaggaacact
     6121 cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac aaccagaaat
     6181 aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga ttggctcgat
     6241 gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc ttttatattt
     6301 aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg aatttttacg
     6361 cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc tcttttttct
     6421 gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat tttagcaatc
     6481 agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt attcatcaat
     6541 ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat actggcattg
     6601 tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa cggttataat
     6661 ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt cctactattg
     6721 cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact agaagaatta
     6781 tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc gttgcttctg
     6841 attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg gacattcgaa
     6901 gtatatcagg caaatccgca attatctttt taccaattgc gccattctag tttcgctatt
     6961 gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat gggaagttct
     7021 ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt gatcaatgaa
     7081 aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc actacttgtt
     7141 cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga acaactcaag
     7201 atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt gcataatatg
     7261 gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat gaaagaaaga
     7321 gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg cttagattta
     7381 ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc agacaaggta
     7441 actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact tgaaaacggc
     7501 gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga tcaagaaaac
     7561 ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc gcgtctagat
     7621 gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca ggaagcattg
     7681 atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt agcacagcgt
     7741 cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact gttagaagaa
     7801 caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc tgagaaaaaa
     7861 gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc aacagataca
     7921 ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa attcttgaag
     7981 aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa agatgtcagc
     8041 ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta tgaagctcct
     8101 gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa aacgtgggac
     8161 agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg tgctgaacca
     8221 tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat ctccttccaa
     8281 attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg agtatacgac
     8341 cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga agctaaagat
     8401 atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc aacgatgctg
     8461 gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt agattctatt
     8521 atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct ttctgtgtct
     8581 ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa agtagaagca
     8641 agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt ggaaggacat
     8701 gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt agttcgtgag
     8761 aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg tgaaatcgcg
     8821 caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga taaataaaga
     8881 ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa atagcttccc
     8941 ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc gtttaccaag
     9001 agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa aaagctcgtc
     9061 acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac cgattaagaa
     9121 gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga gacatgatga
     9181 aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta gcaggaatag
     9241 gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc ctgagcttag
     9301 ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc ccatctgcgc
     9361 gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac aattatcgtg
     9421 aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat gatgacgaat
     9481 ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa cattcatatc
     9541 attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg ccgttcttgc
     9601 cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga tactgtccct
     9661 gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag cgattcttaa
     9721 aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg gtgcagcgtt
     9781 gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata acagtagaaa
     9841 ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc aggaatgcgt
     9901 tttctattac attggtaatt aaataaaaac gtttttttta attggtagta cttttggcta
     9961 gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga atgtcagaag
    10021 ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca ggcgacgaga
    10081 aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta gagttgactg
    10141 gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa gaaatcagtt
    10201 ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta tgtagtgaag
    10261 atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg atccaggcag
    10321 caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt ttattaggag
    10381 aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac ggtgttttag
    10441 tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga aaaagtgaaa
    10501 cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga gtagatgttt
    10561 atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa catctgattg
    10621 aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt gcggtacgtg
    10681 gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat aagaaatacg
    10741 accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt ccacaaataa
    10801 aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc gcgatgaatt
    10861 tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga ttaagtcgct
    10921 tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag cacaaatcaa
    10981 tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga tcatcgtatc
    11041 aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag gattaaaagc
    11101 agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg gcgctcgctt
    11161 atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa tattcaatat
    11221 ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca cgctgtattt
    11281 ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg caccaagtgt
    11341 attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag catatggacc
    11401 agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca ttgataatat
    11461 gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt ggagtttgat
    11521 tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa aaggcgaagt
    11581 ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag gattacgtac
    11641 agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg ctgtgttatt
    11701 tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac cggattatga
    11761 ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa aatgaaaaaa
    11821 atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc tcttggggta
    11881 ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg ggacatcgtg
    11941 cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta ccagatcatt
    12001 atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa gacgcagatg
    12061 cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa ctagtgccta
    12121 aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag ggaacacata
    12181 aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa gctgttgttg
    12241 ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact acgatcacag
    12301 ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg aataattatt
    12361 ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct ttgaaaaata
    12421 ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat gcaaaggcag
    12481 caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg ggtgccaacc
    12541 cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc acaagtgttc
    12601 attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta gaagaggtgc
    12661 tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg atggaactag
    12721 cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg ctgtatgaac
    12781 aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa aaagaaaatg
    12841 aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa aagcagttat
    12901 tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg caaaggaaat
    12961 gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt tgaaatcagg
    13021 tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag atcactttga
    13081 ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat tattgaaatt
    13141 agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc caaagggttt
    13201 aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg tcgttatgct
    13261 aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg atgattacga
    13321 acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa cttcaaaata
    13381 cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca aaaactttgt
    13441 tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac gttacttatt
    13501 gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa atgaaatcca
    13561 actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc gtaaattcac
    13621 aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta ttgaatatgg
    13681 tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt taggtaaaac
    13741 attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag aaatcaaaga
    13801 agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga ataaaaagta
    13861 gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat agaaaagaga
    13921 gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc gcagcaatcg
    13981 cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct aaaactttag
    14041 aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc acaaaagacg
    14101 tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa ctgctcttgg
    14161 atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct gatttaagtg
    14221 aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc ggcggaatcg
    14281 gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg atgaaatttt
    14341 ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta aaaaaggtgg
    14401 atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt tattgttagc
    14461 accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg atttgatgga
    14521 tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa caggtattgc
    14581 aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta gtgtcaaaca
    14641 aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct tgttaaatct
    14701 taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag atatgaaaaa
    14761 acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca tgattgatgt
    14821 aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag aagcaaaacc
    14881 agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga aagaagctaa
    14941 aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc acaaatttat
    15001 agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt ttctaaaaca
    15061 agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac atatacacag
    15121 tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag agagaagcac
    15181 atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc gtggacggtt
    15241 gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata gctgttgatc
    15301 acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac ggcttcttgt
    15361 gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc ggttggtttt
    15421 tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac gccaagatcg
    15481 aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg agggcttgca
    15541 gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc ttgttttttc
    15601 aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt cactgcttta
    15661 aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt gtctttttcc
    15721 tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt gtaagaatcc
    15781 gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc gttcttttca
    15841 atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat gatctcccaa
    15901 gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt ttgagcatct
    15961 tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat aaaaagagct
    16021 agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg gccagggtcg
    16081 ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc attgattttt
    16141 acgttattca tttggcaacc tactttctaa attaattttg tattcaataa ttgtaccact
    16201 gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc atgaaaacag
    16261 agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa tgtaagacgt
    16321 attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc aaacaatcac
    16381 tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac gtgtcgtaaa
    16441 tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta tcgatcgctt
    16501 ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa caacagttgg
    16561 tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg gtatcgatga
    16621 tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca acgaccaaaa
    16681 agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga tcttcatggg
    16741 tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc ctgttgtatt
    16801 agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt atgtatccgc
    16861 aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg cttttgtcag
    16921 tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata aagaagcatt
    16981 aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat acaacttcaa
    17041 agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg tctatgtttc
    17101 agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg tgaaagtacc
    17161 ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag ctcgtcctcg
    17221 tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc gcctattgac
    17281 gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt atggaatcga
    17341 agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt gcctgccgtt
    17401 taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga tgaatattgt
    17461 gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg gttatgacaa
    17521 aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt gaaattacat
    17581 ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac catcaaaaaa
    17641 ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt ctattaaatc
    17701 aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa gctgaagcgg
    17761 tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta aaggcgcttt
    17821 tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt tggatggatt
    17881 ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact tttttcttca
    17941 tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt tttgcttggt
    18001 aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct ttgctgtcca
    18061 ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga gtagagacaa
    18121 ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa tctgcttttc
    18181 ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt tttgcattga
    18241 tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag atccggacag
    18301 agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa tcgatgatat
    18361 agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc tgttgaagcg
    18421 tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca tttgccgggt
    18481 agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg gaattcaaca
    18541 catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac tcacggacgg
    18601 tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca gcattttctc
    18661 cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata agccccatgt
    18721 cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga cgttctgtat
    18781 cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat ccgtagatgc
    18841 ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca gcaccatcat
    18901 ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca tctttatcga
    18961 ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt tgataataag
    19021 cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat tgttgtgcaa
    19081 tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca gtctgttctg
    19141 cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct tcgtattgtt
    19201 ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt ctggcaaggc
    19261 cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac gtgatcgggc
    19321 tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt gcatctacac
    19381 caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta ttattacgac
    19441 caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag tatttttcta
    19501 cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca tctgtaagga
    19561 tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca ataccagtag
    19621 cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt ttatcgaagt
    19681 attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta tcagatgaaa
    19741 tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct gcataaacaa
    19801 tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta gtcggcaatt
    19861 gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct agagctgcga
    19921 acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta aaaataatct
    19981 taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta tttttgcgtg
    20041 gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat aatagaacaa
    20101 aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta aaacgtgaga
    20161 aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt tcagttacta
    20221 tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta ccaatatata
    20281 ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt aggctcacac
    20341 gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc cagttatgga
    20401 tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa agccatcgaa
    20461 gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac aaatatcgtc
    20521 gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat gtttcctttt
    20581 gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc tactcaaatt
    20641 acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc tagaattatt
    20701 gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt ggagacaatg
    20761 gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat catcgatgga
    20821 gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa tgatgccggt
    20881 tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat catcgggtta
    20941 gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc gcataaagac
    21001 cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa gatcgtccat
    21061 gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg caaagataaa
    21121 aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca aacattcgat
    21181 ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt tctatctgac
    21241 agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa acaaaggagt
    21301 actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat ttagacaaac
    21361 aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg gcaaaacgtg
    21421 attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa ctaaaaaaga
    21481 aacatacata ctttattctt ggcatagccc tattatttac aattattcgt ttgtatttat
    21541 atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag ctgctgattc
    21601 gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact acaacacttt
    21661 ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat ctttcttatg
    21721 cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc gcgtttaaac
    21781 cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg ttttctccga
    21841 ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg attccggctg
    21901 ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt tctagcagaa
    21961 aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg ttcattcgcg
    22021 aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt ttcgtccaga
    22081 tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa cgactctttc
    22141 ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata gttggctttt
    22201 ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt gcaaccatca
    22261 atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga atagatgatg
    22321 aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca ctaaataaag
    22381 cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa ttatatacga
    22441 ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc atcttttggg
    22501 cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa gaaaccaatg
    22561 atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga acaatcaaaa
    22621 agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt gatctgccaa
    22681 tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa gatttccatc
    22741 aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa gaattattaa
    22801 accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct aatttccaat
    22861 taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt ctggctattc
    22921 ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc gtatttgcaa
    22981 aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta atactgacac
    23041 aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa aaaaaggact
    23101 attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc gtagtaacaa
    23161 gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa taaaaagacc
    23221 cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa atcagttttt
    23281 attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt cctttatata
    23341 aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca taagcgatgt
    23401 gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt tcgatctgtc
    23461 ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag ccaagaagtt
    23521 ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg gtcatcatca
    23581 gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat tcattcatta
    23641 agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac aaaacacaaa
    23701 tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa atcgggatac
    23761 gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta atagtagagt
    23821 tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca gattttgcta
    23881 cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca tacagaccaa
    23941 aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg atcaatagca
    24001 gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta gaaaaacgta
    24061 cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt gaaactgctg
    24121 aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa gctccagtca
    24181 gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta gggataaagc
    24241 cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca aaaacgaata
    24301 aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg acttttgtca
    24361 gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc aatggaatat
    24421 caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct gttgccataa
    24481 tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg cgtgctcgga
    24541 ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata tactgtaaaa
    24601 gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg aaattttgtc
    24661 ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct actaacgcac
    24721 caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt acatcaactg
    24781 ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga tgcttatgaa
    24841 attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc tacgatt
//
LOCUS       DAZKBH010000028        24576 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000028 DAZKBH010000000
VERSION     DAZKBH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.028"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..551)
                     /locus_tag="ACG8OO_001147"
     CDS             complement(153..551)
                     /locus_tag="ACG8OO_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9045038.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="ACG8OO_001148"
     CDS             complement(548..1321)
                     /locus_tag="ACG8OO_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9045039.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="ACG8OO_001149"
     CDS             complement(1318..2001)
                     /locus_tag="ACG8OO_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9045040.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="ACG8OO_001150"
     CDS             complement(2168..3055)
                     /locus_tag="ACG8OO_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9045041.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="ACG8OO_001151"
     CDS             complement(3052..3921)
                     /locus_tag="ACG8OO_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9045042.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="ACG8OO_001152"
     CDS             complement(4058..5344)
                     /locus_tag="ACG8OO_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9045043.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="ACG8OO_001153"
     CDS             5617..6195
                     /locus_tag="ACG8OO_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9045044.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="ACG8OO_001154"
     CDS             6195..7124
                     /locus_tag="ACG8OO_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045045.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="ACG8OO_001155"
     CDS             7121..8857
                     /locus_tag="ACG8OO_001155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9045046.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACG8OO_001156"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACG8OO_001156"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFP9045047.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="ACG8OO_001157"
     CDS             complement(9753..10262)
                     /locus_tag="ACG8OO_001157"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFP9045048.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACG8OO_001158"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACG8OO_001158"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9045049.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="ACG8OO_001159"
     CDS             complement(11563..12654)
                     /locus_tag="ACG8OO_001159"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFP9045050.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACG8OO_001160"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACG8OO_001160"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9045051.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACG8OO_001161"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACG8OO_001161"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9045052.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACG8OO_001162"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACG8OO_001162"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFP9045053.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="ACG8OO_001163"
     CDS             complement(16617..17591)
                     /locus_tag="ACG8OO_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFP9045054.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="ACG8OO_001164"
     CDS             complement(17572..19455)
                     /locus_tag="ACG8OO_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFP9045055.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="ACG8OO_001165"
     CDS             complement(19473..21698)
                     /locus_tag="ACG8OO_001165"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9045056.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="ACG8OO_001166"
     CDS             21813..22847
                     /locus_tag="ACG8OO_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9045057.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="ACG8OO_001167"
     CDS             complement(22914..23927)
                     /locus_tag="ACG8OO_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9045058.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="ACG8OO_001168"
     CDS             complement(24005..24193)
                     /locus_tag="ACG8OO_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9045059.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7062 a         5341 c         4397 g         7776 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atgcaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg atgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cccaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taacta
//
LOCUS       DAZKBH010000029        23986 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000029 DAZKBH010000000
VERSION     DAZKBH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.029"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..640
                     /locus_tag="ACG8OO_001169"
     CDS             71..640
                     /locus_tag="ACG8OO_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9045060.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(818..1258)
                     /locus_tag="ACG8OO_001170"
     CDS             complement(818..1258)
                     /locus_tag="ACG8OO_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9045061.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1416..2180
                     /gene="map"
                     /locus_tag="ACG8OO_001171"
     CDS             1416..2180
                     /gene="map"
                     /locus_tag="ACG8OO_001171"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9045062.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2212..3135
                     /locus_tag="ACG8OO_001172"
     CDS             2212..3135
                     /locus_tag="ACG8OO_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFP9045063.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            3211..4350
                     /locus_tag="ACG8OO_001173"
     CDS             3211..4350
                     /locus_tag="ACG8OO_001173"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFP9045064.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            4343..5143
                     /locus_tag="ACG8OO_001174"
     CDS             4343..5143
                     /locus_tag="ACG8OO_001174"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9045065.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            5143..5970
                     /locus_tag="ACG8OO_001175"
     CDS             5143..5970
                     /locus_tag="ACG8OO_001175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9045066.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            5975..6682
                     /locus_tag="ACG8OO_001176"
     CDS             5975..6682
                     /locus_tag="ACG8OO_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9045067.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            6782..7648
                     /gene="rfbA"
                     /locus_tag="ACG8OO_001177"
     CDS             6782..7648
                     /gene="rfbA"
                     /locus_tag="ACG8OO_001177"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9045068.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            7662..8234
                     /gene="rfbC"
                     /locus_tag="ACG8OO_001178"
     CDS             7662..8234
                     /gene="rfbC"
                     /locus_tag="ACG8OO_001178"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFP9045069.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            8256..9284
                     /gene="rfbB"
                     /locus_tag="ACG8OO_001179"
     CDS             8256..9284
                     /gene="rfbB"
                     /locus_tag="ACG8OO_001179"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9045070.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            9382..10233
                     /gene="rfbD"
                     /locus_tag="ACG8OO_001180"
     CDS             9382..10233
                     /gene="rfbD"
                     /locus_tag="ACG8OO_001180"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFP9045071.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            10268..12301
                     /locus_tag="ACG8OO_001181"
     CDS             10268..12301
                     /locus_tag="ACG8OO_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045072.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            12345..13625
                     /locus_tag="ACG8OO_001182"
     CDS             12345..13625
                     /locus_tag="ACG8OO_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFP9045073.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            13835..14641
                     /locus_tag="ACG8OO_001183"
     CDS             13835..14641
                     /locus_tag="ACG8OO_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9045074.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            14653..15873
                     /locus_tag="ACG8OO_001184"
     CDS             14653..15873
                     /locus_tag="ACG8OO_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9045075.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            15863..17449
                     /locus_tag="ACG8OO_001185"
     CDS             15863..17449
                     /locus_tag="ACG8OO_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9045076.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            17488..19626
                     /locus_tag="ACG8OO_001186"
     CDS             17488..19626
                     /locus_tag="ACG8OO_001186"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9045077.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(19994..20977)
                     /locus_tag="ACG8OO_001187"
     CDS             complement(19994..20977)
                     /locus_tag="ACG8OO_001187"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9045078.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            21355..22746
                     /locus_tag="ACG8OO_001188"
     CDS             21355..22746
                     /locus_tag="ACG8OO_001188"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9045079.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            22762..23802
                     /locus_tag="ACG8OO_001189"
     CDS             22762..23802
                     /locus_tag="ACG8OO_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9045080.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            23821..>23986
                     /locus_tag="ACG8OO_001190"
     CDS             23821..>23986
                     /locus_tag="ACG8OO_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFP9045081.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERL"
BASE COUNT         7673 a         3996 c         4927 g         7390 t
ORIGIN      
        1 aaaaaaacat gttacaatga aattaatcaa tggtggaagc gttataaaaa aattaaggag
       61 attaaaaaat atggcaagaa aaaagacgat tactagagaa cagattttgg cagctgctta
      121 tgaagtagct gccaccgatg gatttacacg atttactgcg agaaacatcg cgaacaaaat
      181 gaattgttcc acgcaaccga tttatcttga gtttaaaaat atggatgact tgagacaagc
      241 tctttttgag cagatctatg atcatttaca atatgaagtg ttcactgttg agcatacagg
      301 gaatacgatt atcgatcttg cttttaatta tatccatttt gcaagaagag agaagaagtt
      361 gtatcgttca ctgtacttag aagaatatgg tggaggaaaa gagatgcaga atttctctta
      421 tacttacttt gtgaatacag tgaaacgcga tccagcttat gtgaatcttt cagaagagca
      481 gatcgaatct ttgtataatg ggacatggat catagctact gggatcgcag ctttgatgtc
      541 ttcaagcatc atccatccaa ctgatgaaca gattgaaaag atgatccaag acagtataga
      601 tgctatcttg aaattcaaag aaccaatcga aatcgattga atatactcaa aagttccaca
      661 aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag ttcgtttttc ttacagcgta
      721 tgcattttta actcccgaag aactcttcca tgaatgcaat gaataaaaga gccatacaga
      781 ttgaataaat ctggatggct cttttattgc aagtatatta aatttttgcg actagttctt
      841 tagcgaaagc ttctaaattt tcgatatctt cttcttcagc attcagatcc actttaacat
      901 tttcggctcc ttttgatgcg cctgtttttg caaaagcagc atcgaaatca tccaccgatt
      961 tgcagaattc atcgtaaaaa gtatcaccag atccgcagac accgtaaatt tttcccaata
     1021 gatccaattc ttgcaagtct tcataaaagt ctacgatctc atcaggcaga tcgccatcat
     1081 cgtatgtata agtagctact acgcaaatat ctgcctcctc gaattcatct gcatctactt
     1141 gggtacattc gttgatttcg acttcgatat ccagattttc aaatgcttct gcaacaatat
     1201 cggcgatttc ttcagtgttt cctgtcatgc tagcatatac gatttttgct aaagccatga
     1261 tgtgcctcct cgtttataat tatttaacaa ttatgaatta taccaaaaca tcattgaaaa
     1321 gaaaataaaa cctaaagaca cctggggaat gtcatttggc taaatgtatg ccgctatgtt
     1381 aaaatgagag ggatttgtaa aaaggagacg aaaatatgat tactttaaaa tcagcacgtg
     1441 aaattgaaat gatggcagaa tctggagctt tattagcaga tgtacataaa aatctgcgtg
     1501 attttataaa accaggaatc acaagttggg atattgaggt atttgtcaga aactatatcg
     1561 agagtcatgg cggaattgct gcgcaaattg gctttgaagg gtatgaatat gcgacttgta
     1621 tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa aaagcctttg aaaaacggtg
     1681 atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg catttctgat tcttgttggg
     1741 cctatgtagt tggagaatct acacctgaga tcgaccgatt gatggaagta acgaagaaag
     1801 ctttgtacat cggtattgag caagcacaag tagggaatcg gattggtgac atcggtcatg
     1861 ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt tcgtgatttt attggtcatg
     1921 gcgttggtcc tacgattcat gaagaaccag cagttcctca ttacggagaa gctggaaaag
     1981 ggttgcggtt gaaagaaggc atggttatca cgatcgaacc tatggttaat accggaactt
     2041 ggaaaatgaa aatggatcct aacggctgga ctgcgtatac aagagacggc ggactttctt
     2101 gccaatatga acatacattg gctattacta aagatggtcc aagaattttg acatcacaag
     2161 gagaagaagg cacttattga tctatccagt caataggtac gggagaggta catgaaagtt
     2221 tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta ttgagacgac tcaaaagcgc
     2281 atcactgatt cagaaatgag tacgacctct gtcgtcgttg cttattattt actcttgtct
     2341 ttatttccat tgattattgc ttttggtaat attttaccgt ttctccacat cgatcaagag
     2401 actgtactga cctatttacg agaagtgatt cctgagaccg tttaccagtt tatcggtcct
     2461 gcaatcaaag accttttgac acaaagttca ggaggcttgt tatccgtttc tgcattagcg
     2521 gctttatggt ctgcaagtca aagtatcaat gctttgcaaa ttgcgatgaa taaagcgtat
     2581 ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct tctcactaat tgtcattgtc
     2641 ttgttcatga ttgctattag tggggtaact atcgttcttg ggttgggaca gctgattttg
     2701 gatgctattc agccaatcgt acaaataccc gtgaacttta tcgatcagtt ccaaactttg
     2761 aagtggccaa tcacattggt agctttattt gtcatcatgt tcttgattta tttaattgtc
     2821 cctaatgctc agttgaagtt gaagacgatc attccgggca cgatcttcgc tactgtcggc
     2881 tggatgctgt tatcacaagt gtttagtatc tatgctaggt attttgcaac gaggatcagc
     2941 ggttatcaga tcataggaag ttttattgta ctcatgcttt ggcttaattt tgctgctacg
     3001 atcatcattt tgggcggaat catcaatgca gttgttcaag aatacgtgac gggtcatcaa
     3061 atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata aattgaaaac taaattttct
     3121 cataaaaaaa agtagaaaca agagttctta ttgtaaattt atatcagtaa tttataataa
     3181 ttatgactct taattaaagt aggtgcccat atgcttgttt cttttagtat ggttataaga
     3241 ttttttctga caattttatt atcactgata ttaacacctg tattcaaaat catttcagta
     3301 caaacaggta tggtcgacaa accaaatgaa agaagaataa ataaagttcc aatgccttca
     3361 gcgggcggac tgcctatctt tatttcattt gttatatcga ctttattctt attccgtaaa
     3421 attatccctc aatcgtatat tgtaccgatc ttgattgctt ctttagtaat catagtcaca
     3481 ggagtgctcg atgataaata tgagctgaca cctaaacaaa aaagtatagg gatcttgatt
     3541 gcttctctta ttatttattt tgtagcagat atacgtattg attccttcac gcttcctttt
     3601 attggatata tccaactagg ctggcttagt tttcccgtaa cgattttttg gatcttcggt
     3661 atcacgaatg cggtcaatct gattgacggt ttggatgggc tggcggcagg gatttcattg
     3721 atcggtttaa ttacgatagg aatcattgga tattttttcc tccatgcttc tacagtgtat
     3781 attccagttg ttattttctg tcttgtagca agtattatcg gtttttttcc ttacaatttt
     3841 tatccggcaa agatttattt aggcgataca ggagctctat tcctagggtt tatgatggcc
     3901 gtcctttcac tacaagggct gaagaacgtg acttttgttt catcgatttc tttgctgatc
     3961 gtcatgggag tgccggtaac agacacgttt tttgctatca ttaggagaaa agcaaaccgt
     4021 gtttcttttt ctacagcaga taaaaaacat cttcatcacc gactaatcag tttaggcttt
     4081 actcataaag gtgctgtcct gacgatttat gcgatggctt tgatgttttc tttcacagct
     4141 atggtcatga attatactgg aagaatcgga acgatcgtat tgattattgc catgttgttt
     4201 gctgcaattt tattatttga attgattggt ctgatcaatg agaaacatca gtttatttta
     4261 aaaacattgc gatttttagg gaataaagag taccgtacgc aagtaaaaca gaaatatttg
     4321 aagaaatgga accgtcatat agatgaataa caaattaaac cagccgatcc ttattagtat
     4381 tgtgacggca aacagtgata aaatttttcg gacactagat aattttattt caactgtaaa
     4441 agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat tcacttcccg agtacaaaaa
     4501 aagactaaca gagtatagga aatatcccta catcgatatc tattttcatg atgaaaattc
     4561 cggatttggt tatggacata atcacaatct gatagctagc gattctcgct atggcattat
     4621 ttgtaaccca gatattttag tggatgaaga gacagttaca gcattggtct tgttattgaa
     4681 agaacatccg gaatgtgcga tgttagcacc taagattctt aatgaagatg gaacaactca
     4741 gcacctcatc cgtgagcagc tggctgtttt tgattacgtt ttgcgctttg tgcctttcaa
     4801 atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt gaatgccgag gattgcctac
     4861 tgatgctaat agttatgtac ggatgatctc aggtagtttt atggtagctg atattgataa
     4921 atttaaagag ataaaggggt ttgatgagcg tttcttcatg tactttgaag acaacgactt
     4981 gtgtttgagt tttgaaaaag caggttacaa gttattgtac acaccctttt atgcagttat
     5041 ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg tttcgcgtgt ttttgacttc
     5101 tatgatgaaa tttttcaata agtgggggtg gcgttttttc tgatgaaacc agaaatatta
     5161 gtagtcgttg tgctttatca gcaactattc agtcaatctc cagcttatgg accattaagc
     5221 aaagcactta gcgaaaagca aattcagctt attatttacg ataatagtcc tcaaaggcag
     5281 caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc atgatccgca aaatcctggt
     5341 ttagcggtgg cttataattt tgcgttgtct caagcagatg aaggaacacg ttgtctcgtc
     5401 actctggatc aggatacgag gctagtcgac gattattttg aactattgcg taaagtaacc
     5461 tttacagatg aatgtgttgc tgcagttccg atgatttttt ctgggagccg acaagtttct
     5521 cccctatatt cagatcgtta tatcaatcgt acagctcaag cagtagaagt tgaaggccca
     5581 acagctgaac gaatcatggc aatcaattca ggtgttgcct ggtctgtcaa atttctaaaa
     5641 aagattggcg ggtttaatcc tgctttttct ttagattttt tggatcattg gctgttttgg
     5701 aaagtggatc agttggaaaa aactgtggag atccttcctg tacgaatgga acatgatctt
     5761 tcggttttag actatgaaaa agtttcaagt aatcgatacc gttctatttt acaagcagaa
     5821 aatcgatttt atcatgaata tgatcaaagg catttatcca gtcaccgacg acaattattg
     5881 ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc atatctggcg gatgactttg
     5941 cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt tcggtttgta tagctacctt
     6001 taatggaggc agatatttaa gagagcagtt agatagtatt ttatctcagc tttcagcaga
     6061 agatgaagta gtcatttctg atgatgggtc agtagatgat acatgggata tattaactga
     6121 atacgcagaa aaagataagc gaatccagct tttttcgggg cctcgtcaag gattgatcgc
     6181 taattttggt tatgcgatcc agcagacgaa aggagatcta atctttttgg cagaccaaga
     6241 tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac tattttgagc agaatccgga
     6301 tcagctagta gtagtttctg atttaatcat tgtggacagc gagctgcaag agatccatga
     6361 ttcgtatttt tcgtttcgaa attccaaaga aggatggctt aataatgtga taagaagtca
     6421 ctatattggt gctggtatgg ctttccggtc ttctttaaaa cccgatattt tgccgatccc
     6481 gtcagaagtg ccgatgcatg atatgtggat tggtttgctg ggaggaaaga agactgggtt
     6541 cctaagagaa ccgttaactc tgtaccgaag acatgattta aacgcgagtg agattgaaac
     6601 acattctaaa ttacggcaaa aaatttcttg gcgtgttcat ctgattacgg cgttgatcaa
     6661 acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc ttaatatttc catgacaaat
     6721 ataacaattg atgagataat aacaaagtga aatttgaaat caaaaaggag agcagatatc
     6781 catgaaggga atcattcttg ctgggggcag cggaacacgt ctgtaccctt taacaaaggc
     6841 aacatcgaaa caattgatgc cgatttatga caaaccaatg atttattatc caatgtctac
     6901 tctgatgttg gcgggaatca aagaaattct gattatttca acgccacaag atacaccacg
     6961 tttcaaggaa ttatttggaa acggaaatga tttaggactg cagatcgaat atgcagtaca
     7021 agaaagtcca gatggattgg cacaagcgtt tatcattgga gaagaattta ttggagatga
     7081 tagtgtctgc ttggtattag gggacaatat ttactatggt ggcggtctgt ctaaaatgtt
     7141 gcaacgagca gcttcaaaag atacaggcgc gacagtattt ggctatcatg tcaatgatcc
     7201 agaacgcttt ggtgtagtag aatttgatga gaatatgcaa gctttgtcga ttgaagaaaa
     7261 gcctgctcag ccaaaatcaa attacgctgt aactggcctt tatttttatg acaatgaggt
     7321 aatttctatc gctaaaggta tcaaaccttc agaacgtgga gaactagaga ttacggatgt
     7381 caataaagca tatttggata aaggaaaact atccgtagaa cttatgggac gcggatttgc
     7441 ttggcttgat acaggaacac atgaatcatt gcttgaagca tctaccttta tcgaaacaat
     7501 tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa attgcttacc gtatgggcta
     7561 catcgataag gaacaattgc tggcccttgc acagccattg aaaaaaaatc aatacggtca
     7621 atatttattg aatttggccg ctgaataagg agtccttaca gatgaaagta acagatacaa
     7681 aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg cgatcatcgc ggtttcttca
     7741 cagaaagtta ttcaaaagca aaatttgcag aacatggatt gaaatatgac tttgttcaag
     7801 acaaccattc actatcagct gaagctggtg tccttcgggg gcttcatttc caaaaaggag
     7861 aagctgcaca gacaaaattg attcgggtag tcactggagc agttttagat gtagtagtgg
     7921 atatccgcaa aggtagcccg acttatggtc aatgggacgg atttatctta tctgaacata
     7981 atcatcgaca gttgcttgta cctaaaggat atgcccatgg attcgttact ttgacaccaa
     8041 atgtgaactt cctttataag tgtgataatt actataatgc agaagcagat gggggcatcg
     8101 catttgatga tccagattta gcgattgatt ggccaattga tccatcaaga gcgattatgt
     8161 cggaaaaaga tcaccgtcat ccgacattaa aagaatttga agcagaaaat ccatttgttt
     8221 acggagaaat ctaagaatta aggagaaatt ttcttatgaa aaacatcatt gttaccggag
     8281 gtgccggatt catcggctct aatttcgttc attacgtggt aaacaatcat ccagaagtgc
     8341 atgtcactgt tttggataaa ctgacttatg caggaaacaa agagaacttg gccggacttc
     8401 ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga tgcagaatta gtggaccgtc
     8461 tagtaaaaga agcggacgca gttgttcatt acgcggcaga atcccataac gataattcat
     8521 taaaagatcc tttcccattt gtacaaacga atatcattgg tacgtataca cttttagaag
     8581 cttgccgcaa gtatgatgta cgctatcatc acgtatctac tgacgaggtt tacggcgatc
     8641 ttccattacg tgaagacctg ccaggacatg gggaaggcga aggagaaaaa ttcactgcgg
     8701 aaacaccata taatccttca agtccatatt cttctacaaa agctggttca gatctgttag
     8761 taaaagcatg ggtgcgttct tttggcttac gtgcaaccat ttcaaactgt tcaaacaact
     8821 atggtcctta tcagcatatt gaaaaattta ttcctcgcca agtaacgagt atcttaagcg
     8881 gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg tgactggatc catacgaatg
     8941 atcattcttc agcagtgtgg ctgattttga cgaaaggcag aattggcgaa acctatctga
     9001 tcggtgcaga cggtgaagaa gataataaga ccgtgatgga attgatctta gaaatgatgg
     9061 gccaaccaaa agacgcatac gaccatgtaa acgaccgcgc agggcacgat ttgcgttatg
     9121 caatcgattc gacaaaattg cgagaagagc taggttggca gcctgagttt acgaacttcc
     9181 gtgatgggtt ggctgacacg atcaaatggt acgaagaaca tgaagactgg tggaaaaaag
     9241 aaaaagaggc cgttgaagca gcatatgcca aaaatggaca ataatacaga ggaaatttca
     9301 ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc tgtctctttt ttaaaataaa
     9361 aatcaaggag cggataacca tatgttttta ttaactggag gaaatggaca attaggtaca
     9421 gaattgcgcc atttattaga tgaaaaaaat ctgccatatg tttcaacaga tgcaaaagac
     9481 ttggatatca cagatgctga gaaaacaatg gcttatatta cagaattaaa acccgatgtt
     9541 attttccatt gcgcagctta cacagcagta gataaagcag aagaagaagc aaaagaatta
     9601 gatgagaaaa tcaatgtcga tggcacaaga aatgtagcac ttgcagcaaa agctgtgaat
     9661 gcacaattcg tctatatcag tacagactat gttttcgatg gaaaaaacaa aaatgtagaa
     9721 tacgaagtag acgaccaaac gaatccacta aatgaatatg gtcgtacgaa attattaggc
     9781 gaaaaagcag tagaagagat tttagaagac tattatatca tacgaacttc ttgggttttc
     9841 gggatctatg gacacaattt cgtcttcacg atgcaacgcc tagcggaaac ccgcgacaaa
     9901 ctgactgtag taaatgacca gtttgggcga ccaacttgga cacgtacttt ggcagaattt
     9961 atggtatatg tcatcgaaga aaaagcacca tttggtattt atcatttgtc gaacgaaaat
    10021 agctgttctt ggtatgagtt cgcaaaagaa atattgaaag atacagatgt agaagttgca
    10081 cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc cgcagtattc agtcatgagc
    10141 ctgaaaaaga cagaagcgtt aggttttgtc ataccaactt ggcaagaagc acttgcgcaa
    10201 atgctggaag ctgccaaaaa aatgaataag taatttcctt tttatcaaat agaatttgga
    10261 gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa aagcgaatca
    10321 ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta ttggcgtttt
    10381 agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg tttgggataa
    10441 atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac agaatcggga
    10501 agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag ctgaagagga
    10561 cttttcgttg gtgaacgagg attattcatt aaacggacaa aacatgatca ttgcgtataa
    10621 ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat tctttttcct
    10681 cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca tggtcttgct
    10741 tggattcgaa gtactgatga tattaacgaa gaggaaaaaa gctctatcgc taatcggtgc
    10801 atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg tgggcgattt
    10861 gattttcttt actttgggac tgatgattgc tttttatcat tatttctaca atcatgataa
    10921 aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg ggtttatttt
    10981 agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt attttgttgg
    11041 cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag tgttgattgg
    11101 aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt caaaagacgc
    11161 attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg gaggaaagtg
    11221 gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg aagatatcaa
    11281 ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg tattcctagc
    11341 aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg gacgtatttt
    11401 gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa aatggctagc
    11461 agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt ttagttttgc
    11521 ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg ttattcctat
    11581 ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg cgattacgca
    11641 ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg tgttgggaac
    11701 agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg cttttgtcgc
    11761 cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag gagcgatttt
    11821 tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg gtaaagtttg
    11881 gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat ccttcaatac
    11941 agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag aagaatacga
    12001 gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag atacaaagtt
    12061 cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg cagaaaaatt
    12121 agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat tatatgaagc
    12181 gcagttcacg cagctatatg gaccggataa agacggttat atgattttta aagtagaata
    12241 tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag tcgtacagta
    12301 ataggctagc tgagaataat cctgaaatat tagtagaaag gagagtgaag ataattcaag
    12361 caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg ttgctgacgc
    12421 tagtcttttt tgtttggaca gcaggggtag gttttccagc aactttgttt atatataaca
    12481 atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat gtttataaag
    12541 caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt ttcttttctt
    12601 actttcggga attaagaaat gagtctttat ttggagactt actgtttcct ttggttattt
    12661 tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt atttatctga
    12721 ttgtctttac ggtcttcctc ggaatcacgt tatatcgtgt atttacagag atccaagtac
    12781 cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg atcaatacga
    12841 atacaatcgg aagttctttg atgactcttg ctttattgat cagtggattt gccagttctt
    12901 ttgaacgatg gtatgttcga atcatcggtg ggatagcagt cgttgcagcc tttttaggta
    12961 tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg atattggatc
    13021 ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcctac acggttacga
    13081 ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc aatttgttta
    13141 ccgatcgaga agatatttgg cataaattct atcagacgct aggagaaaaa tctgaacaag
    13201 ttttactagg aatgaaaaca tttatcttcc agcgaggaaa ccaattttta ggaaaccaca
    13261 atagttataa ttctattttg aatatctatg gattgatagg atttgggatt gctgctttgc
    13321 tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac gggcagatga
    13381 cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat acattgactt
    13441 catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat cgatatgatg
    13501 aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat atgacttatg
    13561 atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc caacgctatc
    13621 attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt tttggtttat
    13681 tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg tatcatagaa
    13741 aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat ggcacttgac
    13801 tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt atttatttat
    13861 gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg acttcaaatc
    13921 aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc tatttactgt
    13981 attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg atgcaaaata
    14041 cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct cagatgcatt
    14101 tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag tcgtattcaa
    14161 tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccatt tattcttcat
    14221 tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa tgtctttaca
    14281 gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt ggctgacggc
    14341 atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc tgcagttgat
    14401 catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc ttgtaagcat
    14461 attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat ttttaagtga
    14521 agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca cgttagtaat
    14581 gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag atgtgctgta
    14641 aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa atcttataat
    14701 atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa gaaatcctat
    14761 catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg tgagatgatc
    14821 ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat tacgggagta
    14881 ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt gctagaacta
    14941 ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa tggaatggtt
    15001 ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga atttgctgac
    15061 atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt cgttcgtcta
    15121 gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga agcattggca
    15181 gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat caagaactcg
    15241 ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt ttgtgatcgt
    15301 tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga agtaacgacg
    15361 aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa taaaaaccaa
    15421 gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt gttggataaa
    15481 gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa agtaacttat
    15541 gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg aacagtcgac
    15601 aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc ttggaaaaaa
    15661 ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg tgaatattat
    15721 tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa gacaaagtac
    15781 atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt agaccataaa
    15841 tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag atgaacaaag
    15901 cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag agtttggtcc
    15961 cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag tgagcattgt
    16021 tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg gattttatgg
    16081 tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg attacgtctt
    16141 gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga aagtcagaga
    16201 atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc ataattctgt
    16261 catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc tagatgaaac
    16321 ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag cgggattatt
    16381 cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa aatcaactta
    16441 tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg gtgaagtcta
    16501 ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg cagagccaca
    16561 aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag atgaaacaat
    16621 ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt tccccgtatc
    16681 agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta tcgaattcag
    16741 cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag tcaaaacagt
    16801 aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa cggatatttc
    16861 tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga caccaacaat
    16921 gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc tttctgagaa
    16981 aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa gactagacca
    17041 tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg aatggacaat
    17101 aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag aaaaagaact
    17161 ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa aaatcatcgg
    17221 atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag ctgaccaatc
    17281 gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag aacagctcgc
    17341 aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac gttcattctt
    17401 agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa taatgattaa
    17461 cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt atttatcgat
    17521 caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc aattgatgaa
    17581 gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc tgaagcaaca
    17641 catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac ccaaagcggt
    17701 ttcaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt ccaaacagcg
    17761 agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga tgacggcatt
    17821 gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta tgcaaggact
    17881 catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg atcgatcgac
    17941 tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag gaaagaagca
    18001 caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa cgttgaaatc
    18061 aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca ttgggaacta
    18121 tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga atcctatcaa
    18181 gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat cagtctggca
    18241 actaattctg cattggaaat agcagaagga gaatttatag cactcttaga taatgatgat
    18301 gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca tccagaactg
    18361 gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt tgatccgcat
    18421 ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc tcacttaggc
    18481 gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta tgaaggttct
    18541 caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca tatttaccac
    18601 attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag taatggcgaa
    18661 gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct gtctagaaga
    18721 aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat tgcttatgac
    18781 gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta tgaagatcta
    18841 aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga aatcattatt
    18901 gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata caagcatcag
    18961 ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc tcgaattaac
    19021 aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa cgatacagaa
    19081 gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga tcgaatcggc
    19141 tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg agtattatta
    19201 ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca ttgtggttat
    19261 ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg tatgatggtc
    19321 aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt agcattcaac
    19381 gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta tgcacatcaa
    19441 gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc agaaaaacaa
    19501 agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata tattgcgcat
    19561 gatccatttt acaatgataa tttaacaaaa gacggaatcg gcgacttttc gttgagacca
    19621 gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac ggctttttat
    19681 agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg aggttgggac
    19741 agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt caaatttttg
    19801 tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt cgtttttaaa
    19861 gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct cagagcgcca
    19921 atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa tcgatatttt
    19981 attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca aaagccactc
    20041 tatcaattat catgggttta aaataagaag tgatggagat aaccagattg ttcttagcgt
    20101 caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc tcagaatcta
    20161 atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca ctggttctag
    20221 gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta tacatccctt
    20281 tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac aaaccatatc
    20341 ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga gacttatcca
    20401 ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg tttggtatcc
    20461 ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta cttgtctgat
    20521 acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg ccggttatac
    20581 cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt gaacagactt
    20641 ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag tgcttgattg
    20701 taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa taatctaata
    20761 ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc agcaaagata
    20821 caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa ccattccatt
    20881 catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata tttggttgtc
    20941 gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc actttcttct
    21001 ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg taatacaggt
    21061 attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata taaaggttca
    21121 ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta ccaacattag
    21181 tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt agaggtgtat
    21241 aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac taggttttta
    21301 aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt atatatgaat
    21361 aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga tgttattgta
    21421 tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat cccattacga
    21481 aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat tgcgttgaac
    21541 attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt gtttgtcaca
    21601 gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc gggccgctgg
    21661 tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct tttcttaagt
    21721 atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa aaaggtcgtc
    21781 attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc aaagaaaaac
    21841 aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat caaaaatatg
    21901 attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga aaaaatcaaa
    21961 atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac atttgaaaac
    22021 ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat tgaagcatct
    22081 ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat catcgtttca
    22141 cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt agtcaaagtg
    22201 acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca aaaggaattt
    22261 tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg accagtttta
    22321 gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc tgtccgcttt
    22381 gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt aggaccacgt
    22441 cctgaacgtt ctttcttcgt cgatcagttc aatgcagaaa acccttatta ctacttacgg
    22501 cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata tgtctctgat
    22561 tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt gatgcttgat
    22621 ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc tcaaggactt
    22681 gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga aaaaatcttt
    22741 cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt accagtatac
    22801 aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga ttatgaagag
    22861 tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat cattcaagaa
    22921 ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg gcccggatta
    22981 gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt cgatgcagat
    23041 gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa gagtccaaaa
    23101 gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag aatcgtttcc
    23161 gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt tataaaagac
    23221 atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg ctatcgtagc
    23281 gatattctga aaaataacca aattctattt aaaacttata atagttgtga ggatcgaata
    23341 tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa agtagaatat
    23401 atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa caagttcgaa
    23461 acatttaaag aattctattc attagcaaat actgtaacag acgatattaa taaagacggt
    23521 atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta tagtacctct
    23581 gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga tccagtgatc
    23641 cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac aaagttgctt
    23701 tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt cgttgaagtg
    23761 aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga ggaaaatata
    23821 atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga gtctttcatg
    23881 atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt gatttatgaa
    23941 gattatgcag atgttcaaga ctatcattca gagattgaaa gattag
//
LOCUS       DAZKBH010000030        23510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000030 DAZKBH010000000
VERSION     DAZKBH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.030"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1663
                     /locus_tag="ACG8OO_001191"
     CDS             2..1663
                     /locus_tag="ACG8OO_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9045082.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1733..2080)
                     /locus_tag="ACG8OO_001192"
     CDS             complement(1733..2080)
                     /locus_tag="ACG8OO_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFP9045083.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2164..3105
                     /gene="opp3b"
                     /locus_tag="ACG8OO_001193"
     CDS             2164..3105
                     /gene="opp3b"
                     /locus_tag="ACG8OO_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9045084.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3106..4149
                     /locus_tag="ACG8OO_001194"
     CDS             3106..4149
                     /locus_tag="ACG8OO_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9045085.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4155..5225
                     /locus_tag="ACG8OO_001195"
     CDS             4155..5225
                     /locus_tag="ACG8OO_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9045086.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5215..6168
                     /locus_tag="ACG8OO_001196"
     CDS             5215..6168
                     /locus_tag="ACG8OO_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9045087.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6180..7190
                     /locus_tag="ACG8OO_001197"
     CDS             6180..7190
                     /locus_tag="ACG8OO_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9045088.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7475..8296)
                     /locus_tag="ACG8OO_001198"
     CDS             complement(7475..8296)
                     /locus_tag="ACG8OO_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9045089.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8393..8671)
                     /locus_tag="ACG8OO_001199"
     CDS             complement(8393..8671)
                     /locus_tag="ACG8OO_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045090.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8939..10948
                     /gene="metG"
                     /locus_tag="ACG8OO_001200"
     CDS             8939..10948
                     /gene="metG"
                     /locus_tag="ACG8OO_001200"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9045091.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11427..12014)
                     /locus_tag="ACG8OO_001201"
     CDS             complement(11427..12014)
                     /locus_tag="ACG8OO_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045092.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12189..13511
                     /locus_tag="ACG8OO_001202"
     CDS             12189..13511
                     /locus_tag="ACG8OO_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9045093.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13666..14442
                     /locus_tag="ACG8OO_001203"
     CDS             13666..14442
                     /locus_tag="ACG8OO_001203"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9045094.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14435..15004
                     /gene="rnmV"
                     /locus_tag="ACG8OO_001204"
     CDS             14435..15004
                     /gene="rnmV"
                     /locus_tag="ACG8OO_001204"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFP9045095.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            14997..15881
                     /gene="rsmA"
                     /locus_tag="ACG8OO_001205"
     CDS             14997..15881
                     /gene="rsmA"
                     /locus_tag="ACG8OO_001205"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9045096.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16332..16961
                     /locus_tag="ACG8OO_001206"
     CDS             16332..16961
                     /locus_tag="ACG8OO_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045097.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17085..18194)
                     /locus_tag="ACG8OO_001207"
     CDS             complement(17085..18194)
                     /locus_tag="ACG8OO_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9045098.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18403..18825
                     /gene="mgsA"
                     /locus_tag="ACG8OO_001208"
     CDS             18403..18825
                     /gene="mgsA"
                     /locus_tag="ACG8OO_001208"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9045099.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19012..19110
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19148..19684
                     /locus_tag="ACG8OO_001209"
     CDS             19148..19684
                     /locus_tag="ACG8OO_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9045100.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19724..21487)
                     /locus_tag="ACG8OO_001210"
     CDS             complement(19724..21487)
                     /locus_tag="ACG8OO_001210"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9045101.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21490..23205)
                     /locus_tag="ACG8OO_001211"
     CDS             complement(21490..23205)
                     /locus_tag="ACG8OO_001211"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9045102.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7568 a         4303 c         4777 g         6862 t
ORIGIN      
        1 aatgaaaaag aaatttacat ttggtttggt tgcggtatgc ggccttgtac ttgctggatg
       61 ttatggcggc agttcagcca caagcagtag ttcagcaaaa agcggttcag cagatggagg
      121 aggcgtattt aacttagtcg tgcctcaaga aatgcctaca gctgacttat cagttgcaac
      181 agatacaatc agttttacag cattgaataa cgtttatgaa ggaatttacc gtcttgatga
      241 agacagcaaa ccacagccgg caggagctag cgagttggca gaagtcagtg aagacggatt
      301 aacttacaaa atcaaattac gagaagacgc aaaatggtca aatggtgatc ctgttacagc
      361 agcggattac gtttatggct ggcaacgaac agtagaccca gctacagctt ctgaatatgc
      421 ttacctgtat gcgccagttg aaaatgcaga agacattaca gctggaaaga aagataaatc
      481 agaactaggg atcaaagcag ttggtgacta tgaattagaa atcaaattga cgaaacaaac
      541 accatatttc caatatctgc ttgctttccc atcatttttc ccacaaaaac aatccgtagt
      601 ggaagaaaat ggggatgcct atgcatctgc aagtgataaa gctgtttata atggaccatt
      661 cacattagaa gattttgatg gaccaggaac agatacagaa tggacttata agaaaaatga
      721 tcattactgg gacaaagata cagtaaaact ttctgaaatc aaagtcagtg tagtaaaaga
      781 atcttctact gcattgaact tattcaaaga tggccaagca gatgacgtga tcttgtctgg
      841 tgagttagca cagcaaaacg caaacgatcc agcttatact tctgttaaag aagcgcggac
      901 aagctatatc gaattcaatc aacgtgaaaa gaattcacca ttcaacaatg tgaacctaag
      961 aaaagctatt tcttattcta tcgatcgtga agctttagta aaacaagtac taggtgacgg
     1021 atcagttgtc tctacaggat tgatcccatc agatatggta aaaagtccaa caacgaacga
     1081 agactttgca aaagaagctg gaaaattagt ttcttatgac aaagataaag caaaagaata
     1141 ttgggaaaaa gcgaaaaaag aattgggcgt tgattcttta gaatttgatt tgatggcttc
     1201 tgatgatgat tcatcgaaaa aagtaatcga atatgttcaa aactcaatcc aagaaaactt
     1261 ggatggcgta acagtcaaac caacacctgt tccattctct gtacgtcttg accgctcaac
     1321 ttcgggtgat tttgacgcag tactaggcgg atgggctgct gactatgcag acccaagcag
     1381 ctttactgat ttgtttgtca caggtaattc atacaaccgt ggtcgctgga gcaatgcaga
     1441 atatgacgaa gcagttaaag cagcttcatc aacagacgct ggcgatgaac aagcccgttg
     1501 ggatgatttc caaaaagcaa tcaagatcat ctctgatgat atgggtgtcg ttccggtgta
     1561 ccaaaaagca gaaggtcacc tagtgaatga aaaaatcaaa ggtatcgttc accatgcggc
     1621 aggcgcttca tgggattaca aatggacata cgttgaagaa taatcgttaa taccgtgagc
     1681 atgagaaaaa acgaggctgc ggcaaatgtc gcaacctcgt tctcatattc acttatccta
     1741 acaaagaaaa aagcatgaaa atagcagaaa gagcaattag tattccggca atgatcagcc
     1801 aaaagctgta cataccgtag ctggcagacg agagcgagga aaattctggt tttttctttc
     1861 ggttccttgt tcgtgcaaga tggaccgatc gctgaaaatg atcgaagaaa cccgaagaaa
     1921 agacccataa aaaaccgccg ataatcagaa acggcaatga ccataagaat agatcattag
     1981 ataattgaat gagtgtaaag gaatggttga tgagattttt tattaaaata accagaatca
     2041 gtagaaaagc tactgcgtag gggatagatt tttttttcat ttgattgctc ctttattttt
     2101 ggttcattat ctaataatgt agcgaaacat ctgtcagaag tcaaaagcta gggggaaaca
     2161 ttagtgaata gttatatcaa atatgtatta aaacgtgtct ttttcatgat catcacgctt
     2221 tggttaattg caacaatcac ctttttcttg atgcaattgc tgccgggcac gccatatacg
     2281 aatcaggaac gtttgagtcc tgaaacaatc gagatgctaa ataaacaagt cggtttggat
     2341 aaaccagtta tcgtacaata tgggatttat ctttctaatt tgcttcaagg agattttgga
     2401 atttccttcc aatttaaaaa ccagccagtc gcacatttgt tagctggaag aatcggacca
     2461 tccttgcagt tagggttaca ggcaattatc tttggaacag tcctagggac gattttagga
     2521 acgatctctg ccatgaagca aaatacatgg gcagatacat cttctacact cgtagcgata
     2581 ttaggtcgtt ccattccaaa ctttgtattt gctgttttat tacaatatat ctttgcaatc
     2641 aaattacaag ttttgccaat cgcaaaatgg gatggctttg tgtacacaat cttaccaaca
     2701 attgcgttgg ccatgtcgcc gcttgctgac tctgctcggt ttatccgtac ggaaatggtg
     2761 gaagtactgc atagtgatta tgtggaatta gcaaaagcaa aaggattgag ccgttgggaa
     2821 atcgccttta aacatggtct tcgaaatagt ttgattccat tgatgacttt attaggaccg
     2881 ttagctgtag cattgatgac cggatcactg gtggtagaaa atatctttgc tattcctggt
     2941 atcggggaac aattcgttaa gtcaatcaca acaaatgatt atcctacgat catggctgta
     3001 acgatccttt actcatttat gctgattttt gttattttga tcgtggatct attgtacggt
     3061 ctagttgatc caagaattcg tgtttcagaa gggagccgag gataaatgga aatgatacca
     3121 aagcgttatg aaacagttgc agatatccct gcggatgaat ttcttccatt acaaaaaaat
     3181 accgaagaag aacgtgaaca gattactgca ccttctttga attttatcca agattcatgg
     3241 cgccgtttaa agaaaaacaa agctgccgtt atttcaatgg ttttactatt ggtcatcatt
     3301 ttgatctctg tgatcacaat ctttgtttct cctcatgatc caactgccca aaacgtggct
     3361 tatatcaatt tgcctcctcg tattccggga atcaatatcg atggattgaa tggaaaagca
     3421 atggttggtg gtgaacttgt tgataaatat gctcaagcaa acgtcccttc aaacgtaaat
     3481 ttctttttag gaacagacgg tttgggacgt gatgtattaa gccgcttatt tatgggaatg
     3541 agaatctcgt tgctgatcgc atttattgct gcattactag atgtaacgat tggtgtagct
     3601 tacgggttgg tttctggttt gttaggcgga cgtgtcgata acgtgatgca acgttttttg
     3661 gaaatcttat caggaattcc aaacctcgtt gtcatgattt tgatgttggt cgtttttgaa
     3721 ccaggaattt tttcaatcgt ggctgctatg gccatcacaa actggatacc aatggcacgt
     3781 atcgttcgtg cacaaacaat gaaattaaaa gatcaagaat atgttttagc tggattgaca
     3841 ttaggtgaat caagaatgaa aattgcattc aaacatattt tgccgaatat ctctagtgtc
     3901 attattattc aaatgatgtt tagtattcca tctgctattt tcttcgaagc ctttttaagc
     3961 tttatcggtt taggtcttac gcctccgtca gcttcattag gaacgatgtt gagcgaaggg
     4021 tacaagacat tcctgtatct gccttatcta ttatggattc cggcagtaac tttatcggtt
     4081 atcatgattg gctttaactt acttgctgat ggattgcgtg atgcctttga tcctaaaatg
     4141 aaagagtgaa gacgatgacg aaaattttag aagtgaaaga cttagaaatc tcttttgata
     4201 cgtatgctgg aaaggtacga gcgattcgcg gtgtcaattt tcatttgaat aaaggagaaa
     4261 cattggcgat cgttggggaa tctggaagtg gaaaatcagt gacgaccaga agcatcatgc
     4321 gcttgctgtc aagcaacgcc aatatagatg ctggtcaaat cctctttaaa ggtaacgata
     4381 tcgttggaaa atcagaaaga gagatgcaaa aaatccgtgg taaagaaata gcgatgattt
     4441 tccaagatcc aatgacctct ttagatccga cgatgcccat tgggaagcaa gtcgctgagt
     4501 cccttagaaa gcataataaa gtttcaaaaa aagagggaca aaaagctgca ttagaattgc
     4561 ttaaattagt tggaataccc gaagctgaga aacgtatcaa cagttatccg catcagttct
     4621 caggaggtca gcgtcaacgg atcgtgatcg cgattgcact ggtatgttat ccggaaatcc
     4681 tgattgctga tgaacctaca acagcgcttg atgtgactat tcaagcacag attttggagc
     4741 tgctgaaaga aattcagaca aaaatcgata catcaattat ctttatcact catgaccttg
     4801 gtgtagtagc gaatgtcgct gatcgtgtcg ctgtcatgta tggaggaaga atcgttgaag
     4861 ttggaacatc ggaagaaatt ttctacaacc cgcaacatcc ctatacttgg ggactgcttg
     4921 gttcaatgcc gacattagat agtgcgaacg atcgattgta tgctattcct ggttctccac
     4981 cggatttatt gaacccgcca aaaggagacg cattctatcc aagaaatgaa ttcgcgatga
     5041 aaatcgatgc agaacaagaa ccaccatttt ttgaactatc aaaaacacac aaggcagcaa
     5101 cttggctact tgctccgcag gcacccaaag tgacaccgcc agaagaaatc caacgtcgct
     5161 gggctatctt taaagagaaa caaaaacata cttacggtca aggaggaatt gctgatgcca
     5221 aataaactac ttgaagttaa aggattgaaa cagtatttca atgtaggaaa aaagaatgaa
     5281 gtacatgctg taaatgacat cagctttcac atttatgaag gagaaacatt cggtttagtt
     5341 ggagaatctg gaagcggaaa atccactact ggccgtacga tcattcgctt gaatgaacct
     5401 actgatggtg agatattatt cgacggtcaa gatataacga agatcaaaga taaaaaaggt
     5461 ttgacgaaat tccggcatga tgttcagatg atctttcaag atccatacgc ttcgttgaat
     5521 cctcgtatga aagtcagaga tatcattgcc gaaggaattg atgttaatgg actagctaaa
     5581 tcatctgaag agcgtgcaaa aaaagtcgat gatctattga gaactgttgg attgaatcca
     5641 agccacggta ctcgttatcc tcatgaattt tcaggtgggc aacgccagcg tattggtatt
     5701 gcacgagcat tggcagtaaa accgcgtttt atcatttgtg atgaaccaat ttctgcacta
     5761 gatgtgtcga tccaagccca agtggtcaat ttgttgcaag atctccaaaa agaacatcaa
     5821 ttgacctatc tatttattgc acatgatttg tcgatggtta aacatattag tgaccgaatc
     5881 ggtgtaatgc ataacggctt gttattagag atgggaacaa gtgatgaaat ctataatcac
     5941 ggtgttcatc cttatacaga aagtttgtta tctgctattc ctctaccaga tccagaccat
     6001 gagagaaaac gcagaagaat caaatatcaa cctgaaccag atgacggaca agtacgtcaa
     6061 cttcgtgaaa tcgcaccaga acattttgtc tatgcaactg agcaggaagt cccatattac
     6121 gcaaaaaaac taaaacgaca aaaggaatct cttctggttg ctaactagga gggacataga
     6181 tgctaaaagc ttttaaattc cgtatgtatc cgacagaaga acaaaaacag caattgattc
     6241 gtacttttgg atgtgctcga tttacctata accatttgct gaaaaagcgg cagaaaagtt
     6301 ggcaacaaac aggagtagcc aatttttcgc tcacacccgc tacattaaaa aaagaatacc
     6361 cattcttaaa agaagtagac agtcttgctt tagcaaatgc acagcttaat ttggatcggg
     6421 cttttcgcaa ttatttcaag ggacgtgcca gcttcccaaa gcttaaaacg aaaaaaagta
     6481 tgtggcaatc ttatacgacc aacaaccaaa cccgtacgat ctatctggaa aatggctact
     6541 taaagttacc aaaacaaaaa gaactaatca agataaatca gcatcggcca gttgaaggat
     6601 cgattcgctc cgctacgatt tctgctcgat ataatgaaga attttatgtc gcactacttt
     6661 gtgatgtttc gccagtaaaa aaagaatctt tagctaaatg gatcgggatc gcctatcatc
     6721 cgaagacatt gatccaaaca tctcgacctc tagaagtaac tttaccaaaa tttcaccaga
     6781 cagaagaaaa gctgcaacat gcacagcgga aattgaatgt gaaagtccgc tccgctcacc
     6841 atagaaagat caggttagat caggcaagca attatcagaa acaaaagaga aaagtaatgg
     6901 atctttatct gaaacaaaag aaccagcgag aagattatct tgaacaatta tctgggaaat
     6961 tggtcaaaca atatgactac ttatttgtag aatcttttcc aaaagaagag gcacatgctg
     7021 atttttctat tcacgactgg cataaattaa taacaaaatt gcgttataag tcccaatggt
     7081 ataataaaaa atttcttttg atcaataccg acggagcaga agaaagcaat tccgtaagaa
     7141 agagccaagt agtagaaaaa atgggcagac attcggttat caagggataa aaaatgaatg
     7201 aaactgatag gagcgttagg gttagccttg ttaataaaaa gtcttaatga cttttgtttc
     7261 taggaatttt tcttttgaag aaaagctcac taattatgac aaaccaataa aacaatagca
     7321 gaaaaagttc gtacgagctt tttctgctat tgttaatttt ttattcagaa agtttacgaa
     7381 tacatcaaaa ttctgaaaca aaaaacgtaa ctcacaaaga gttgtttttg cgttttttct
     7441 tgaatgatta ggtctgaatc ttttgctcac aaccttattc atggtgcccc ggcttgagct
     7501 gatagatttt tgaaggacgg ccgattccta caggacgttc accgatctca tcgaaatatt
     7561 ggagcatagc tttcttaaaa ttggaatgat cgatcgtttt ataatcgatc cctaagaatt
     7621 tagcaaatac ttttcgagct tccgtaatcg tgaaatcttt acctaaaact tgtagaactt
     7681 gcggctcgtg ttccatttta ttgactacac ggttgaatgc tttcaagatg atttgactat
     7741 ggtcaaaagc caaagtatct ttgccagaag actctccagt gactaaatca agcgagatag
     7801 ccacttctcc gctagataaa tggatcttgt tactatgacg ttctagtgtg aaccagcgta
     7861 cttccttggc atcatctccg gcgattagcg gttcttctcc aataaaggct aagtagctga
     7921 ctgtgacgac ccagcctcgc ggatctcgat taggcgtact gaatgtatgc agttgctcaa
     7981 tattttcttt tgaaataacc acaccagttt cttcctttgt ttctcttaag acgctttctc
     8041 cagttgactc atccttttgg acaaagccac caggcaatgc ccatgaattt cgataaggat
     8101 gcccttttct ttggatcaac aaaactttca gctgatcttc ttctttgtta tagcacatca
     8161 gtacgatatc gacagtcaaa gagggcttgt catattctgg acgttcctgt ttttgatacc
     8221 agttaagaaa ttcttcttca cttgctttat gttcataata taatttttct tcttctttag
     8281 aagcaaaacg gttcatctga ccacctccat tgacagtaaa agtacttttt accttattgt
     8341 agcagactct gaaaaaaaac caaggattta cttgaaaaat ctaggttatc atttaaatat
     8401 gaaaaataaa tggaagtatg agattgaata aaaagaaaag cagataaaac ggcaatactt
     8461 tataccgctc ttcacgaatg gcaaagaagc cacaaataac gatatatacg tcaatgataa
     8521 aacgcaaaat ccaagctgct gtcataaaac tgttcaaatg cccatggagg ttaaaataac
     8581 catacatacg aatataaaaa tagctgaata ttaaatgtgc taatgaaaga aaagcaatac
     8641 taagtgtaaa aaaaagatgt ttcttctgca tgattgttcc agtcccttac taagatagat
     8701 agtctttatt ataccacgtg tgaggggaga aataaaaagc cgttttttag tagatatgaa
     8761 acaaaacaaa aacccgcatt tattcaacct attcatcctt ttaaaggata gattgaacgt
     8821 agatagaagt cgtttatcca gtctttttca ttttgaaaaa aagctaacag catgcttttt
     8881 ttatggtata ctagcaagga aacgaattcg catcatgcga taaaggagga agagccccgt
     8941 ggcagaaaag gaaacttttt atatcactac gccaatctat tatccaagcg gcaaacttca
     9001 tatcggcaac tcgtatacta cgattgcatg tgacgcaatt gctcgttata aacggttgat
     9061 gggctttgat gtgttttatc tcactggagt ggatgaacat ggtcaaaaaa tcgaaacaaa
     9121 agcagaagaa ctaggtgtaa aaccgcaaga atatgtagat aaaatggcgg ctgatgtcaa
     9181 gaaattatgg aaaacgcttg atatcagtta tgataaattc attcgcacga cagatgatta
     9241 ccataaagca gctgttcaaa aaatatttga tcgtttatta gaacagggag atatttactt
     9301 aggtgaatat gaaggctggt actctgtttc tgacgaagaa ttctttacag aaacacaact
     9361 tgcagaagtg tacaaagatg acgaaggtaa cgtgatcggt ggtaaagcgc caagtggaca
     9421 cgaagtagaa ttggtaaaag aagaatcata cttcttccgc atgagcaaat acgcagaccg
     9481 tctattggaa tactatgaag aacatcccga attcatccag ccagaatctc gtaaaaacga
     9541 aatgatcaac aactttatca aaccaggatt agaggatctt gcagtttcac gaacaacttt
     9601 tacttgggga gtaccagtca agagtgatcc gaaacatgtg gtgtatgtat ggatcgatgc
     9661 attatccaat tatattactg cactaggata tggctccgaa gatgaaagtc tattcgaaaa
     9721 atactggcct gcagatgttc agatggtcgg taaagaaatc gttcgctttc atacgatcta
     9781 ttggccaatc atgctgatgg cccttgattt gcctctacct aaaaaagtat ttgggcatgg
     9841 ctggctattg atgaaagacg gaaaaatgtc taaatcaaaa ggaaatgtcg tttatcctga
     9901 aatgcttgtg gaacgttatg gtttagatgc actccgttat tatttgcttc gtgctgttcc
     9961 gtttgggtca gatggtgtct ttacgccaga agattttgtt tcccgtgtca attatgatct
    10021 ggcaaatgat ctagggaact tattgaatcg aacgattgct atgatcaaca aatattgtga
    10081 tggactcgtt cctgactatg cctcacttgt cactccattt gatagtgaac tgtctacgac
    10141 ggctgctaac gttgtcggac gttaccatga tgcaatggaa aaaatggaat tcaatacagc
    10201 attggctgaa atctgggtgc tgatttctcg tgcaaacaaa tacattgatg aaacagaacc
    10261 atgggtatta gcaaaagacg aagaaaagaa agctgaattg aatagtgtca tgatccattt
    10321 agctgaatct ttgcgtatcg cagcgatatt acttcagcca atcatgacag aaacgccaga
    10381 gaagatcttc aatcaactag gattggactc tgaaacaatg aatcttgaag gattacactt
    10441 tggcgaattt ccttcaggaa caaaagtagt tgcaaaaggt acaccaatct tcccacgttt
    10501 agatatggaa gaagaagttg cttttattca agaaaaaatg tctgaaggta ctcaaacaaa
    10561 cgaagataca gtcaaatggg atccagaaga aacagaactt gtttcaacga aagaaaaaca
    10621 aatcaaattt gatgtatttg aaaaagtaga actaaaagta gcagaagtca tcaactgtca
    10681 aaaagtagaa ggggcagata agttgctgca attccgacta gatgctggag acagccaaga
    10741 ccgtcaaatc ttgtcaggaa tcgctgaatt ttatcctgat ccaagcgaat tgatcgggaa
    10801 aaaagtcgtc attgtagcaa atctgaaacc acgaaagatg cgtggacaaa tcagtcaagg
    10861 aatgatcctt tctgcagaag caccagatgg ctctttacaa gtaatcgaag caccaaaatc
    10921 catgccaaat ggctcagaaa tcgcatagaa attcaaaggg ggtatgacac tagttgtgtc
    10981 ataccccctt tttccgaaat ttgagaagca attttcgtaa attccttctt attacttgaa
    11041 gccaatcata taatacagaa acagagaact atccaaaata aatcctagaa aaagaagtac
    11101 gagcttcgct gatttttgtt aatttagaat aatactaata ggaatcgtaa aaacagaaaa
    11161 aaagcataat gataaagggt cgggcagatt ttaattgatt catctggtac tcttttttat
    11221 tgtccttaca ttcataaagt ttgaatgcaa tgactttatt acatctatat ttatatctaa
    11281 cagtagcgtt gatgtgttaa ttataagttc tatcacttta tgttcaatta gtttgtgctc
    11341 ttatatgtgt gattgaaaat atagataaaa aaacaatcat cggctttgac ttcgatgatt
    11401 gttttcttta ttttgtaaaa atcctatcac ttgattttat ggtaaccttt tatttttgtc
    11461 ttcaaacggc ttggtaagag agcagtaagc agtgcaaatg ggatcgaaaa gacataccag
    11521 aaaatcgtac gtccgatacc ataatgttga tagagctttg tcaacatcac gtgtaacaag
    11581 atcaacccta aaagcaagga gccagctata aaccaatgat tatcccaatg catgacttca
    11641 ctaagtgcca tcactccaaa aggatagaaa taaaaataga tcccgactaa ggtcatccgc
    11701 cagttctttg taaatgttcc gatattcaat aaaaagaaaa tcgctgaaag caaaataccg
    11761 ataggcggaa aaagcccaac caaaaaggaa tacaacatcc cccacaagat catcacagga
    11821 cctttgatca atgcaacgat tgcgatgaac aaagtcagca atattagttt ttgtgataac
    11881 gttgcaacag tcattaatcc aaataaaatt aatgtgaacc aatgccaatg ggactgctgt
    11941 tgttctttac gggaaaaatc gacccggttg gataaataat ctgctaattt cggataagaa
    12001 cgcttaaaat ccataaactg ttcctccttc gtggaaaagg taggtttagt atacgaaaaa
    12061 taaaaaagaa atacatccgt cttaagaggg agtttttcac tgaaagtgga gaaaaaagaa
    12121 tggtaaatta aaatattctc agttatactg aaaattatac caaatttcaa agaagaaaga
    12181 ggatgggaat gaaaggcaaa ctaaaacgag aaatcaatct gtttggcgca ttagctaccg
    12241 ttatgggaac cgtgatcggt gcaggcgtct tcttcaaaac tgctgctgtt actgctagta
    12301 ctcagtctac aagtttgaca ttgctagctt ggcttttggg tggtttctta acgatctgtg
    12361 ctggattgac agtggctgaa ttagctacag caatcccgga aactggtgga gctgtaaaat
    12421 atattgaagc tgcgtatgga aaattgccca gctttttatt aggatgggcg caaagcttga
    12481 tttactttcc tgctaatatt gctgcattat cgattatctt tgcgacacaa ttgacgaact
    12541 tattacaatt atctaccgat tatcttttgt tgattgcagt gatcaccgct gtttctgtga
    12601 cagggttgaa tttgttaggg acaaaagtcg gtgcttctgt acaatcagtg acgttgattg
    12661 ttaaattaat accaattgct gtgattgtca tatggggatt attgacccca ggacaaggaa
    12721 ctgtccagtt atttccaatc gaagctggaa aagatgttac ttttgtcgaa ggattgagca
    12781 gtgcgttgtt agcaacgttg tttgcttatg acggctggtt aggtgtcggt gcaatggcag
    12841 gggaaatgaa acggccggaa aaagatttgc ctaaagcaat tattcttggt ttgagttttg
    12901 taacagtcgt ttatctattg atcaattttg tttttttgaa aactttgccg attgatcatc
    12961 ttgccggtaa tttgaatgca gcttctgaag catcagacgt catttttgga ggaatcggtg
    13021 gaaagctggt cactattggg atcttgatct cggtatacgg tgcattgaac ggttatacac
    13081 taactgggat ccgcgtgcct tatgcgatgg ctttagaaga cgagctacct ttcagtaaac
    13141 agctgacaaa gctttcaaag aagtttacgg ttccttatgt tcctgcggtt ttccagttag
    13201 cagtagcttg tattatgatg agccttggtt ctttcgactt tttaacagac atgttgattt
    13261 ttgtgatgtg gctattcagt ttactgattt ttatcggtgt ctttatttta agaaaaaaag
    13321 cgccggaatt gccacgtcca tataaagtac cactttatcc aatcgttccg attattgcga
    13381 ttctcggtgc tattttcatt ttaggtatga cgatgatgac gcaaacgaaa ttagcattga
    13441 tcggtatagg tgttaccttg ataggtatcc cagtctatta tcaaaaaaag aaaaaacgct
    13501 cagaagagta aaatatttca acaacagaca gaaaaagtac gaactaggtc ctctagatct
    13561 tcgtgctttt tctatttcaa taaaacaagt tcttctattt tgatttttgc tacttctgca
    13621 agtagtgctt atgtgctaaa gtaagaatga aaaggagaga taaacatgat atttgattct
    13681 catacccatt taaatgctga acagttcaat gaagatattc cagaaacgat tcagcgagca
    13741 caagaaatgg gggtaacgaa aatggcggtc gtgggcttcg atacgccaac gattgaaaag
    13801 tctttgcaat taagccatga ctatccaaat atatacagta ttatcggctg gcatccgaca
    13861 gaggctggca gttatacgaa agaaatcgaa aataaattac aagaacaatt gactttacca
    13921 aaggttgtag cattagggga aattggcttg gattatcatt ggatggaaga cccgaaagaa
    13981 gtccaagata aagtatttcg cagacagatt gctatcgcaa aagaaatgaa cctaccaatc
    14041 agtattcata ctagagaagc acttgaagac acgtatcaaa tcctaaaaga agaagatgtt
    14101 cgagatatcg gtggaatcat gcatagtttc agtggtgatt atgaatgggc aaaacgattt
    14161 cttgacttag ggatgcatat ttcatttagc ggtgtcgtca ctttcaaaaa agcattagac
    14221 gtccaagagg ctgcagcaaa tgtaccaatc gaacgattac ttgtggaaac agatgcccct
    14281 tatctggcac cagttccttt tagaggaaag cgaaatgaac caggctatac tcgttataca
    14341 gtagaaaaga ttgctgaact tcggaatctt ccgacagaag aagtggctgc ccaaacatgg
    14401 acaaatgcgc accgattatt tggtctaagc gaacatggat aagatgaaga tccaagagat
    14461 tatagtcgtt gaaggaaaag acgatacaag aaggctgcgg gaagtagttg atgcagatac
    14521 gatagaaaca atagggtctg caatcaatga agagatcctg atgcaaattg aacatgccca
    14581 agagactcgt ggagtgatcg tttttaccga tcctgatttt tcaggagaaa aaataagaaa
    14641 aatcattatg gaagctgttc ctcaggcaaa acatgctttt ttgccgcgca cacaagctaa
    14701 accaaagaaa aagggaagcc ttggtgtcga gcatgcaagt gatgctgcta ttttagaagc
    14761 attgaaaaag gtcgtgacgc cagacatttc ccaaacggct gtttctgaaa tcactagaca
    14821 agacctttta gactatggtt taatagctgg ggcacaagca aaagaaaaac gagaaaaact
    14881 aggtgacgag ttgcgcatcg gttacacgaa cggcaaacaa ttggaaaaac gtttgaagat
    14941 gtttcgtatt acgccgaaag aactcgccca agcaatgaaa atagtggagg aacaacatga
    15001 gtgaatatcg tgaaattgcg acaccctcta gaacaaaaga gattttattg aaacatggtt
    15061 tttcatttaa aaaaagttta ggacaaaatt ttctgactga accaaatatt ttaaggaaaa
    15121 tcgtagaaac tgcgcaaata gatgaacata cgaacgtgat cgaagttgga ccaggaatcg
    15181 gtgcattgac ggaacaatta gccaagcatg caaaacaagt cgtagcattt gagatcgatg
    15241 atcgtttgat ccctgtattg gctgatacga tgcaacccta tgacaatgtg actattgtac
    15301 atcaagatat tttaaagacc gatttgtcaa cagctgttag agaaacattt catgaggagc
    15361 ttccgttaaa agtcgtagcg aatttacctt actatattac gacaccgatc atgatgcatt
    15421 ttcttgaatc agatttggtt gtggatgaac tagtcgtcat gatgcaaaaa gaagtagctg
    15481 atcgtatctc tgctgaacca ggaacgaaag cttatggttc attatcgatt gctgttcaat
    15541 attatatgga agccagttta gcattcatcg taccaaaaac agtatttgtc cctcagccaa
    15601 atgtagattc agcgattttg aaactgacaa gaagagatat accggcagtc gaagtgacag
    15661 acgaaaaagc atttttccga ttgacaaaag cggcatttca gcaaagaaga aaaacgcttt
    15721 ggaataactt gcagcattcg tatggcaaag atgaccagac aaaagcatgg ttagcaaaaa
    15781 gcttggaaac agctggaatc gatccaaaac gtcgtgggga gactttgtca ttacaagaat
    15841 tcgccgcatt aagcaatgcg atgtcagaaa atatgcagta aaaaaagagg ttgtgacaga
    15901 agttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
    15961 atgtttttcg tttgtttggg cttgttaccg cagatgcaag cttttgaaca ccgtttaaga
    16021 aagaggttgt gggcctgccg tttaactcta agtcacaagg aacaatttca aaaaacagct
    16081 tctcatgttt ttgagttagt tggtcttatt acttgatgca agcttttgac cactattatg
    16141 ccgaacaagg agatgtgaca caaattttgt cacatctcct atttttgttt cttgatcaaa
    16201 aaattcttat acttgatact cacactcatt ttgtatggat ttagaaaaga atatgtgtct
    16261 tgacagttat tagtaagtga gttatactaa gtcgacattt atgtgtcaag acatattagg
    16321 agagagaaaa aatgagaaaa aattcagttc gaaaactaac gatttctgct ttattgattg
    16381 ggatgggggt cattatccca atggtcatgc ctaaaatcat gattccacca gcttccttta
    16441 ctttggcgtc tcatgtgcca ttatttattg cgatgttttt tacaccagga gttgcagtgg
    16501 ctgttgcgct aggtacgaca tttggtttct ttttaacaac gccggttatc atcgcattgc
    16561 gagcattgtc acatcttgtg tttgcgttga ttggggcttg gtacttgcaa aagcatccga
    16621 gtatcgtttt aaaaaatgga cagtttactt ttgctaattg gcgtttccaa gggttcaatt
    16681 ttgtgatcgg tgtgattcat tctttagcag aaatgctagt agtcagcatc ttttacttta
    16741 ttggtaatat gcctgaaaca tattattctc aaggatattt ttatacagta ttcatcttga
    16801 tggggattgg tgggcttatc cacagtttag ttgattataa tattgcttat tttatagcag
    16861 gaacactcag caaatatttt gatatccccg tatttacggc agcaaaaaaa gaaaatcgga
    16921 aagagtcgat tcaagggtac gcgaagaaaa cgacatatta gacatgatct ctcaaaaaaa
    16981 aaactctcat ctagtaatgg ataataaatc ctctaagatg agagtttttt gtcctaaaaa
    17041 gagctgcacc aacatcttct ggcacagctc taacaggggg caatttatgg aagtttgatg
    17101 acttgttctg tttctttatc aaagaagtgg cctttgctga tgttgaaggc aaggtcgact
    17161 atttcgcctg gtttatggaa atctcttgca tctacttttg agatgaactc agtagaacca
    17221 gtttttgtgt aaagcatcgt ttcagctcca agtaattcgg aaacgacaac ttctgatttc
    17281 actacggctt caggtgaagc ttcaagggct acttgttcac tgtggatgtc ttctggacga
    17341 ataccgaaga taacttcttt tccttcataa cctttttcaa ctaatacttt gttttttcct
    17401 tcaggaatac gaagcttaag cccatgtcca tctgtgataa caccattttt caatgtcaca
    17461 ttaaagaagt tcatggcagg agatccaata aagccggcaa cgaacatatt gacgggtgta
    17521 tcataaactt ctttcggtgt accgatttgt tggataaatc cgtctttcat gataacgatt
    17581 cggtcagcca tagtcattgc ttctgtctga tcgtgagtaa catagatggt cgttgtttca
    17641 agacgctggt gaagtttggc aatctcagca cgcatggcta cacgtaattt agcatctaaa
    17701 tttgataatg gctcatccat taagaagact tttgcatccc gaacgattgc ccgtcccaat
    17761 gccacacgtt ggcgttgtcc accagataaa gcagctggtt ttcgtttcaa gtattctgtc
    17821 agacctaaga tctctgctgc attgtccaca cgttttttga tgtctgcttt atcatattta
    17881 cgtaatttca agccaaaagc catattatca aaaacagtca tatgaggata caatgcgtag
    17941 ttttggaaaa ccatggcaat atcacggtct ttaggtgcca cgtcattcat gacttttccg
    18001 ccgatattta gttctccttc agagatatct tccagtcccg cgatcattcg caaagtagtt
    18061 gactttccac aaccggaagg accaacaaaa acgataaatt cacggtcttt gattgataga
    18121 ttaaaatcag ttacagaata attttctgca ttgtcatatt ttttatatac gtttttcaga
    18181 gccatttcta ccataatacg ttccacactt tcttttttag ttttattaac tatttaaagt
    18241 ataaatgaaa gcgttaacta acttcaatgt catgttgaac aagaattttt tctttttttt
    18301 gtcaatgtgc tagttttaag ctttaataaa tatccataag aaataaaaac tgatacactg
    18361 gagtaaataa gtatgttaac aatatcaaaa ggagtgtctc ttatgaaaat cgctttgatc
    18421 gctcatgacc gaaaaaaacc tatgatggtg aaattagcaa cagcgtataa aacaatattg
    18481 gaaaaacatg aactttttgc taccgggacg actgggcaga ggatcacgga agcaaccggc
    18541 ttgccgatcc atcggtttaa atccggaccg ttaggcgggg atcagcaaat tggagccatg
    18601 atttcggagg atcagttaga tttggtgatt tttcttcgag acccgctggc tgcacagccc
    18661 catgaaccag atgtaacagc acttatccgt ttatctgatg tctatgagat tccgttagct
    18721 acgaatatcg gaacagctga ggtgctacta agaggactgg aagccggctt cctagatttt
    18781 cgaagaatcg tccatgaaat agacaagcat ccactttcat tttgatggat aagcgtatgg
    18841 aaaaataacg tttaatgttc gcctatatgt agctataaaa gctaaacaaa ctgataatat
    18901 gcgatataat acgaacgtat tggataatat aaacaataat attagttttt cgtatttgca
    18961 gtaaatgtga aaatatgcta tagtttctgt gggaaaagat cccaaaaata atacagagta
    19021 gaaaataaag cgttaagtgc taagaggatg ggatgttgcc tcttggacga aggacgtaat
    19081 gttcgcggtt ttattttcgc attcgctgca tgatacgatg tagcagctct taaaggagat
    19141 gctaaaaatg aagaaacacc ggttagacgc acgtatgatt gctattatgg gcttattgat
    19201 tgcattgatg gtcactttgt cccgactggt agcaatcgag accccattta ttaaaatcag
    19261 tgtcacattt attccacaag tgatcatggg cattcttttt ggtccttttt ggtcagggat
    19321 aggcgcagtt ttagctgatt tagtaggaat ggccctcttc tcgaaatctg cattctttat
    19381 tggatttaca ttgaatgcat ttattgaagg agcaatctac ggattctttt tctatcgaaa
    19441 agagatcact tggaaaaatg ccattctggc tacgcttagt gtcacgctga tcatcaactt
    19501 atttttgaca ccattatggc ttgcgttgat gtaccatgtg ccattgttca gctgggtcgt
    19561 ttgggcgccc cgcctgttaa aaacagtaat atggttaccg attcaatcaa ttgccattta
    19621 ttacgtagga cgtagtattc cttacaaaaa aattttaaga agtttagcga tccacgctaa
    19681 ataaaaaaca agcagtcttc ccatcccaga agactgcttg tttttattgt gctgtgaatt
    19741 gtgcttgata gagccgtcgg taataaccat ctttttgaag cagctcatca tgggtgccca
    19801 tttcgacaat agctccctga tccatcacta atatctggtc cgcatttttg attgtggaca
    19861 ggcgatgggc aatcacaaaa ctggtttttc cttccatcat tgctaagaag gcttgttgga
    19921 tttttttctc tgtcaatgta tcgactgagc tggtagcttc atctaagatc aacatagggg
    19981 gattactgat catcgttcga gcaatcgtca acagttggcg ttgtccatct gatatcttta
    20041 acccttgacc gccaatctta gtatctaatt tttccggcaa acgttctaca aattcataca
    20101 tataagaggc ttttagggca tcattgattt ctgcgtcact tgcttccgga ttcccaaaac
    20161 gaagattttc tcgcaatgta ctgtcaaata gccaagtatc ttgtaacacc attccaaaac
    20221 ttttacgtaa tgtatcacgt tggatagagc ggatgtcatg gtcatcgact gtaattgttc
    20281 cttttgttgt gtcataaaaa cgcatcagca aattaacaag tgtggatttt ccggcccctg
    20341 tttttcctac tatagcaatg gtttgacctg gataagcagt aaaattgaaa tcttcgatca
    20401 aacgctgctt ggggtcataa gaaaaagaaa catgatcaaa agagactttt cctttgatat
    20461 ttttgggttc caaggcatag gcatctggca aatcaggctt ttcttctggc tgatcgatca
    20521 aatcaaatgc tcgttctaga ccagaaaaag cggtttgcag ttgtgtcgtg attccagaaa
    20581 gttcgataaa tggtttagaa aactgacttg agtagatcgt aaagctggaa atcacaccaa
    20641 cagtgattga cgaaccactc ttcaatgcca ataggccgcc aactaaacca atcgataaat
    20701 aagcaaggtg atctacaaaa cgagaagacg ggtttgtcaa ggaagaagaa aattgtgccc
    20761 gttgtccttt ttcatataga tcagcattta gcagttcaaa ttgctgttga ctagcttctt
    20821 cttgctgaaa agctttcacg attttctggt taccgattcg ttcggtcaca aagcccgaga
    20881 tatccccaat aatcctttgc tgtctatcga aatttgtttg tgaagcacga gcaacgatcc
    20941 aactcacaag gaaaataatt ggtgtagaac aaagaacgac caatgtcaga agtggactta
    21001 gacgcatcat aaaaataaac gaaagaatga tgattgccac tccagaaaat aattgattga
    21061 aggccgcaga aattgcaatg gaaatattgt ccatatcatt tgtaaatcga ctgacgatat
    21121 ttccgtggga gttttgatca taataacgaa gtggcaatcg attcagatga gcaaatgcat
    21181 cttttcttaa ttttgcaacg gataagtaag ctaaccgatt gcttaacact tggatgagcc
    21241 attgactgac tacgttgatt aaaacgatac caccgaatag aataagtaaa tggaacagtc
    21301 gattgaagcg gacctgtcct tctccaatca tttgatcgat actttgacca atttgtaacg
    21361 tcatcacaac tgttgcaatc ccattgatga ttcctagtat cacagctagc caaatgtctt
    21421 ttgcataggc agtaaggtaa ggaatgaatc ggcggagcgt ctgcaaggat aattttttta
    21481 ctttcataac tattcttcct cctgtgatgc gacgatttcc tgatattctg ctgattgagc
    21541 aagtaattct tcatgggtac cctgagcagc aatcttacct tgatccatca ctaagatatg
    21601 atgtgcttct tgaatagagc ggacacgttg ggagataata atcactgtac tgccaagata
    21661 agtctgtagc gccttacgca aatccaagtc tgtttgataa tccaacgcac ttaaagagtc
    21721 atctaaaatc agtaatgctg gcttggcaat caaagctctt gcaatcgtca gtcgctgttt
    21781 ttgtccgcca gagaaatttt tcccattttc catgactttt gtatctaaac catttggaag
    21841 ctgacggaca aattcttctg cctgagcgat tttcagagct tcccaacatt ctttttctgt
    21901 ggcgtctttc ttaccccatt gaaggttttc acgaatagtc ccggtaaata gtacagaagt
    21961 ttggggaaca agagcaattt gctgacggag atgatccagc tgccaagaac gaacatccat
    22021 tccgtccatt ataactgtac cggaactgac atcatagaaa cgcggaatca attgtgtcaa
    22081 agtcgttttc ccgcttccgg ttggaccaat gattccaagg actgtatttc gcttcagctg
    22141 aaatgtaata ttttggagag aaagaccaga atcaggagta taacggaagt caacatggtc
    22201 aaaagcgata attgcttcag aagaaaaatc gggagtagta ctgacctcag cttcaagaat
    22261 cgaattttct gtgtctagca cctctttcac ccgatttcct gaagcttcgg cacgtgtgaa
    22321 gatcacgacg aggtttgaaa caacgatcag tgccaaaagc atttgattca tataattgat
    22381 caaagcaaga acttgtcctt gttgcaagct accaatattt actttgatcc cgccaactgt
    22441 cagcaaacag atgatcccga cattcatgat caaggttgta gcaggtgata atagggcaga
    22501 gatattggaa acacgtaaat aattgttcgc tagatcattt gtgcttttat cgaacttctc
    22561 tgtttctttt tttgtctgcg caaatgcgcg gatgacacga acaccgctaa gattctgact
    22621 aacgtgttca ttgagctgat ctagtttttc ttgcactttt tggtagagag gaacggtttt
    22681 tttgatgata aaaaataaaa tgattgagaa aataggcaat aatgctaaga aaaacagacc
    22741 tacttcccag tcgatcacaa aagccatgac cactgacccg atacttaaaa acggtgctct
    22801 gatcaataaa cgaatgacca ttgctaaagc tagctgaagc tgattgatgt cgtttgtcat
    22861 tcgggtgatc aaggtatctg ttccaaaatg attcaactcg ttgtgagaga aagtattgat
    22921 ttttttgatt agctgattac gtaactctgt accaaatcct tgtgaagcta ctgaagcata
    22981 gtattggcaa atgatagcac aaatcagacc aatcacagac atagcgacca tccataagcc
    23041 catttcaatg atttttcctc ggtcattcat ttttagtcca ttatcgatca aggaagccat
    23101 cattaaaggc aggacaagtt cgaagacagc ttcaagaaat ttgaagaacg ggcccaaaat
    23161 gatttgctta cgatagtctt ttgcataacg taataactga atcatatgat tcctcctatt
    23221 atttatttgt ccacaattga aaaaacaagt actaccacat tgtagcttag gaaatttaga
    23281 atgaaaagag aacaagcctt ttcataaatg aaagttttat tatgaagaag ctttagaaat
    23341 tgaatagact atttttttta aaaaaaagaa aatgagaaaa catgccagtc tcttcattca
    23401 taggaacgtc tattttactt aatttttcta taaatgaatt ctgcataaaa aatatatcaa
    23461 gtaaatgttt atagatgaaa ctctatttac ttctttttta atttgttctt
//
LOCUS       DAZKBH010000031        23265 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000031 DAZKBH010000000
VERSION     DAZKBH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.031"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..691
                     /locus_tag="ACG8OO_001212"
     CDS             <1..691
                     /locus_tag="ACG8OO_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9045103.1"
                     /translation="TILYNVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYF
                     LSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYN
                     SIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADF
                     GLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTI
                     ARRFDSDSALF"
     gene            703..1623
                     /locus_tag="ACG8OO_001213"
     CDS             703..1623
                     /locus_tag="ACG8OO_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9045104.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1664..3121
                     /locus_tag="ACG8OO_001214"
     CDS             1664..3121
                     /locus_tag="ACG8OO_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9045105.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3466..4080
                     /locus_tag="ACG8OO_001215"
     CDS             3466..4080
                     /locus_tag="ACG8OO_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9045106.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4073..5797
                     /locus_tag="ACG8OO_001216"
     CDS             4073..5797
                     /locus_tag="ACG8OO_001216"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9045107.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5794..7245
                     /locus_tag="ACG8OO_001217"
     CDS             5794..7245
                     /locus_tag="ACG8OO_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9045108.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7718..9871)
                     /locus_tag="ACG8OO_001218"
     CDS             complement(7718..9871)
                     /locus_tag="ACG8OO_001218"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFP9045109.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9873..10919)
                     /locus_tag="ACG8OO_001219"
     CDS             complement(9873..10919)
                     /locus_tag="ACG8OO_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9045110.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11069..12358
                     /locus_tag="ACG8OO_001220"
     CDS             11069..12358
                     /locus_tag="ACG8OO_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFP9045111.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12371..15073
                     /locus_tag="ACG8OO_001221"
     CDS             12371..15073
                     /locus_tag="ACG8OO_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP9045112.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15058..15915
                     /locus_tag="ACG8OO_001222"
     CDS             15058..15915
                     /locus_tag="ACG8OO_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9045113.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15986..16843)
                     /locus_tag="ACG8OO_001223"
     CDS             complement(15986..16843)
                     /locus_tag="ACG8OO_001223"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9045114.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            17054..19225
                     /locus_tag="ACG8OO_001224"
     CDS             17054..19225
                     /locus_tag="ACG8OO_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9045115.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19293..19634
                     /locus_tag="ACG8OO_001225"
     CDS             19293..19634
                     /locus_tag="ACG8OO_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9045116.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19910..21664)
                     /locus_tag="ACG8OO_001226"
     CDS             complement(19910..21664)
                     /locus_tag="ACG8OO_001226"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9045117.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21675..22511)
                     /locus_tag="ACG8OO_001227"
     CDS             complement(21675..22511)
                     /locus_tag="ACG8OO_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9045118.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22513..23196)
                     /locus_tag="ACG8OO_001228"
     CDS             complement(22513..23196)
                     /locus_tag="ACG8OO_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFP9045119.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         7648 a         4158 c         4784 g         6675 t
ORIGIN      
        1 tacaatttta tataatgtag catttatcct tttgaatctg ctgatctcag tcgtatttgc
       61 aatcattatg agcgagttga gaaataaacg actcgttaaa gtttatcaaa cgatgtcctt
      121 actaccttat tttctatctt gggtcattat cagttacttt gtttacgctt ttttaagtcc
      181 tgacaaagga atattcaatc agtggatcac tggacatgga ggagaagctg tcaattggta
      241 taatgaaccg aaatattggc cgtttatctt ggtgttcatt ggtacatgga aagggattgg
      301 ttataacagc attatttact ttgcatctgt tatgggaatc gatccgactt attatgaagc
      361 tgctatggtt gatggagcta gcaaatggca gcagatcaaa catgtgacga taccacaact
      421 tattccacta atgacgattt taacgatttt agccgtagga aacattttcc gagctgattt
      481 tggcctgttt tataacattc ctaggaattc cggtgcatta tatgatgtga ctcaagtttt
      541 ggatacgtat atctacaatg gcctgacatc cacaggagat tttggtatga cggctgctgc
      601 aggactttac caatcagttg tcggctttgt cttgttgatg atcacaaata cgatcgcccg
      661 acgttttgat agcgattctg cattattcta aggaggaaaa aaatgaaaaa aaagaaagtg
      721 gagagagtcc aagtccgctc tttcaataag acggctaatt ttttctctaa tataatgatt
      781 gcactctttg ctatttcctg tattttgcca tttatatttg ttatcgccat ctcttttacc
      841 gatgagtcca tattgtcgac tcacgggtac agtttttggc cagaaaagtt cagtatattt
      901 ggttatacgt acttgttcga ccagatgcag gataaaattt tccaagcgct tttcgtcact
      961 gtacttgtta ctgttttagg tacactgatc aatagcacgg ctacttcttt atatgcttat
     1021 gcgatttcac ggtcaaactt tccattccgt cgttttttta ccgttttttg tttgattacg
     1081 atgttgttct caccagggat ggtggccaat tatcttgtta tgacaaatct tcttcagtta
     1141 aaagatacga tttgggcatt gatcttgcca atggctgtca gtcccttcaa tattattgtg
     1201 atgcgcacat tctttaaacg ttcggtctct gattcgatta ttgaatcggc acggatcgat
     1261 ggagctagcg aactgcgaat atttgttcag atcgttttac cgttagctgt tccaggtatt
     1321 gcgacaatca gtttatttgc cgcattgggt tactggaatg actggttcaa tgctttacta
     1381 tacatccaag atgataagct agttccacta cagtatcttt tgatgaaaat ccaaagtaat
     1441 attcaatatc tgacacaaaa ttcaggagca ggaagccaat tgacaggcgg attagcctca
     1501 gttccaggag aatctgccag aatggcgatc gtagtcatct cgacgttgcc tattgcgatc
     1561 agctatccat ttttccagaa gcattttgtc aaaggtctaa caatcggcgg agtaaaagaa
     1621 taaagaaaca aaaatataaa tcataaaatt aggaggaaga aaaatgaagt tatggaagaa
     1681 actagccttt acaggtgttt cagcgttgtt gttgggaaca ttagcagcat gcggaggttc
     1741 tggttctaaa gaccaggcag aagctagcaa ttcggacact ttgcagatgt atcaaatcgg
     1801 agataaacca gataatttcg atcaattgat ggaagtagca aacaaacgaa tcgaaaaaga
     1861 gatcggtgta aaagtcaata tcaattacat tggctggggc gattacgaaa agaaaatgaa
     1921 cgtcatcatt tcttccgggg aaaattatga tatcgccttt gcgaacaact atgtttccaa
     1981 tgcccaaaaa ggagcctttg ctgatttgac tgaattagcg cctaagtacg ctgaaaaagc
     2041 ttataatgat ttagatgaag catatattaa aggaaatctg gtcaatggaa aattatatgc
     2101 tttcccagtc aatggaaatg tctttgctca acaagtactt acttttaaca aagctttact
     2161 agataaatac gatctttcaa ttgatggaat ccaatcttac gctgatgcag aaaaagtctt
     2221 gaaggaattc catgaaaaag aaccaaacac ggcagctttt gccattggac aaggcttcaa
     2281 agtccaaggg aactttgatt atcctttagg gaatacgtta ccatttgcta ttgatctgga
     2341 tggagatgat tcgaaaatca tcaaccaata cgataatgaa acgtatatcg gactgttacg
     2401 tacaatgcat agctggtaca aacaaggact cattccaagt gacgctgcaa cgagcaataa
     2461 ggattatcca ctagaaggaa atacttggct aatgcgtgga gaaacacaag gaccgtatga
     2521 ttatggcgat acaattttga caaatgcagc aaaacaagaa cttgtctcaa aagcaattac
     2581 tgttccacta aaatctacag ctcaagcaca aatggcaaat tttgtcgtat caaatacttc
     2641 aaaaaataaa gaaaaatcag tagaacttct agggctgctt aacagtgatc cagaattatt
     2701 gaatgggctt gtttggggga tcgagggaga agcatgggaa aaagtcgatg gttcagatga
     2761 taaaatcaaa ttgctagatg gttatcagcc aaatacacat atgtctgctt ggaatacagg
     2821 aaacaacaag attctatata cacaagagtc tattacggat gacatgattg ccaaacgtga
     2881 tcaatcgatt gctgatgcag aaacttcacc gattttagga ttcagcttca acactgacag
     2941 tgtcaaaaca gaattaagta atatttctaa tgtcatgaat caatatttag atggattgaa
     3001 tacagggact gttgatccag atgaaacatt accgaaattg aaagacgctt tggacaaggc
     3061 tggctatgat aaggtattga aagaaatgca aaaacaatat gatgagttcc gacaagaata
     3121 ataatgaata aagacaaagg ggttgtgaca aaagacgtgt cacaacctct tgttccgaat
     3181 aaacggtgtt caaaagccag tttctcggta ttaagctaaa tatatgtaaa aatgtggaaa
     3241 gccatattca caaaattttt cttactactc gaaactaagc ggctatttca caaactcttg
     3301 gtaaacggtg ttcaatcagc taaccctcgg tattcgctca caaaacgaca aaatatgagg
     3361 aactattttt ccgtttgttt ctctaataat caggtcaagg tgctgattgc ccaacctttt
     3421 tttgtataat gagaaagtat aaacttaatg tttggaggag aagatatgtt agggaaggtt
     3481 ttggaagagt tatttatccg gatctgggtc attataaaac taaccttgta tttctggata
     3541 tatacatttg ccggcgggat catctttgga ttaggtgcgg catggaaaac agtcaatgaa
     3601 ctattttatt tgtacggatt cgagtataaa gaaatcacaa taaaaagagg ctggaatata
     3661 tataaaagaa attttttaag aggcaatctg ctatttagtt tatttttatc tggtactgct
     3721 ttattgagtt acaatttata tttatctgtt caaatcaaag ggctcatttt tttagctatt
     3781 gattttattt tactttttgc cttgctctgc ctatttgcta cttatcaata cagtatgatt
     3841 ttagatagtg aatatacgat ctctgttcct aatctgctaa agctaagttt tatttcggtt
     3901 ttttccagct tttcgtcttt tctaaaaata attattggta gcggtataat tttaggcgtg
     3961 acttggcaat tcaaaggatt gatcctgttt ggtgtcattg gtcttttgac ggtttggaat
     4021 ggtacgatga caacacattg gcgtgaagaa ttagataaac agttggaaag ttatgaataa
     4081 gtttcggatt ctattttcaa aaggaacctt actaaatcgt ctgttgcgga aatacgcggt
     4141 tatcatgatc ggtgtaacga tgactgctac cgttattttt agtatccata cttgggaaca
     4201 aaatcaaaaa caggcagaaa acatgacaag tgatgccgtt caaagcacaa gcagaatgct
     4261 gaatgataaa acaaccttga gtaggatcat caaaaaccag cttgtaggga attccgaaaa
     4321 gatcgaaaat gtgacgacct acttaacaaa acccatagat cagtatctga tgtatgtata
     4381 cgaacagcaa aacagtaccg atgaattagt ttctttccca aatcaaatca aagatctcta
     4441 cgcaaattat gaagaactaa gtgcgatata tatcgtgttg aatcaactac cggagtatta
     4501 tgaatcttct agagaaaaca aaggtgggga aataaaaaag gggacaccaa aattactaga
     4561 cgccttttat atcaagttgc ctatcacaca aggaggagct gagtcaggaa gtatttttat
     4621 tggttttcaa aagaaagaac tagatatgat tttagaaaat ctaacgtcat tcaatggact
     4681 ctctttatac atgatttctg gaacgacaaa tcgactatac acattccatg ataataaaat
     4741 agctaaagaa acgtttgaaa gacaagaaat gatcatcaca gagagtctga agaaaaattc
     4801 acaattgccg attgacgatc tggaaaaaaa caattttgtt caacaccaag aactgtctga
     4861 tgattatcgt atattagctg tactagataa aaaaagtgta aaaatcgaaa cgggaaaaaa
     4921 tcttgccccg ctgatcattg gtatcattat tttagacaat atactgctta tctttcttta
     4981 taaaacattc aaacgatatt cacagcaagt atctatcgtc atgcaggcca tggatcaaac
     5041 agcagcaggg aatttagaga cgcggatcga cataacaaac acggaatatg aactgaggga
     5101 actttcaatc ggtatcaacg aaatgttgga tagcatcaat cagtttgtag aagacatcta
     5161 caaattagaa atcaaacagc aagacgcaca tatgcgggcg cttcaagcac agatcaatcc
     5221 acacttttta tataatacat tagaatatat ccgaatgtat gcaatcagcg aaggaagtga
     5281 agaattagca gatgttgtgt atgcgttttc cgctttgctt cgaaacaata cgaatcaaga
     5341 gaaaacgatc actttaaaag aagaactgga cttttgcgaa aaatatgtct atttgtatca
     5401 aatgcgttat cctaatcgag tagcctatca ttttatgatc gatcctgatt tggaaaagat
     5461 cgaagtacct aaatttgtga tccagccatt agttgaaaat tattttaaac acggtattga
     5521 ttttactcga ttcgataatg cgctaagtgt caaagtcttg caggaaggca aacgggtaag
     5581 gatcatcatc aaagataatg gaaaagggat gactgaaaaa cggctaaaac agattgaaga
     5641 aaaactgtct catcctaaag tggaacttca cggttctatc ggtctgcaaa acgtgaatga
     5701 acgtttacgt gccagcttcg gcagcagtta ttatatgtcg cttgagaata atgagacagg
     5761 tggactgacc gtttctatta cattcaagga gggatgagcg tgtatcaagt gttattggta
     5821 gatgatgaat acatgatttt gaatgggtta aaaaagataa ttgattggca aagtttaggc
     5881 tttcaaatag tagcaacagc agagaatgcc aaagaaggac tagctgtact tgaacagcga
     5941 cagattgatt tagtagtaac ggatgtgaca atgccagaaa taaatggact ggaatttatt
     6001 gaagcggctc aaaaagaacg tcataatttt gaatttatga ttttatcagg ataccaagaa
     6061 ttcgattatt tgaaaggagg tatgcaatta ggcgccgtga attatttgat gaaacccgtg
     6121 aataagttcg aattggttga aagcctcaag aaaatcaaaa cacggttaga tcagcaaaat
     6181 gaacaaaaaa atcaacaaga aatctatcaa gaactgttat tcagtcaatg gttgaatgaa
     6241 gaattggatg aaataagtga agaagaaata ataggacaaa taagtgagaa gcagcatagg
     6301 atagtattga ttcaactggc aagaactcat gatactctta tcaatagatg gttgcaagaa
     6361 cggcaacaaa tcttttacta tcaacgaaat tacggagacc gtgtattgat cacgttgttg
     6421 ttggaggcag atgaagttga tggcttctgt tgttttgttc aagaaaactt tcctgatcag
     6481 gaatggctga tcagtatcgg ggaagagaca gattctttgg acaaaatccc tgaaacattc
     6541 caacaagcga aagacaatct acagttgcat caattttatg gagataaaga cgagcatgtt
     6601 ttttacgcag tacaagcaga aacaaaagat caaaccatcg atttttcaag tttcagacgc
     6661 gtgctgcaaa ataaacaatt ggaagtcgct caaaaaatga tcactgattt ttttgaacag
     6721 tttcagttag cagccatgat gccggaagat atacgatatt ccagtttttt actgtttatg
     6781 gagattcaac gggaattgat tgatctggaa gatgaggaat atttacaagg aatcgaaaaa
     6841 atccatcagg caaaaacggt gcaagaactt cagcagctac ttctgtcatt catcaaaaag
     6901 catcagagac agaaaaagta cagtgacaat gtggaaaaag taatcgtaat cctgcaccag
     6961 cattatcaag aaccacttac actcaaagaa gtgtcagaaa acctgcattt aaatgtcatg
     7021 tatctaggac agctgttcaa aaaagaaacg aaaaaaagtt tttcagctta tttgaaccat
     7081 ttacgtatgg aaaaagcgaa acaactgctt ttgcacagta atcaaaacat caatgagata
     7141 gcaagtgaga ttggctacaa caacaccacg tatttctcta aattattcaa gaaaatcgtc
     7201 ggcagatccc ctaaagagta ccgagaaaac agtggagaat cataaaagaa aaagccaatg
     7261 taatgaaata acttataaaa aattgtctag aaatctaatt ttgggctgtt cttattactt
     7321 gaggcaaggc agctttttca tgacctctcg ataaacggag ttcaaaagcc agtttctcgg
     7381 tattaagcca aatttatgca aaaatgtgga aagccatttt cacaaaattt ttcttaatac
     7441 tcgaaactaa acagcttttt cacgacctct ccactaaacg gtgttcaatc agctgaccct
     7501 cggtattagc tcaaaaaatg gcaaaatatg aggaactatt ttgccatttg tctctctaat
     7561 actcaggtca gaacgctgat ttcacaacct ctcaataaac ggtgttcaaa agcttgcatc
     7621 tgcggtaata agaccaacca aacgaaaaat atgaggatct atttttgctt ggctgttctt
     7681 attacttgat gcaggacagc tttttcacaa cctcttgtta attatagttt gataaggaaa
     7741 aaggcaaatc ttttgcttga tatttcttag ggtgagggat gatccctaat tggtaagaga
     7801 tcgtgccgcc tttcagcaaa tcttgatggg taaaatataa tttcgtatat tctctgtcca
     7861 tccgtttgat tttatggata aattgttgct gcggccgatt aggcgttgct tcaatcgtca
     7921 attcctttcc atttgacaaa agcaaaacag ccttgtcaat taatggcata ccgataacat
     7981 attcaccggt tccaggcgtg acaggataaa atcccagact attgaagata taccaagcgg
     8041 ccatacttcc attgtcctca tctccagggt aacccattgg agaatcgtcg aaggtctgag
     8101 tcatcaactg tttaagcagt ggctgtgcca tttcaggttg tcctatataa ctgaacaagt
     8161 aaggatagtg aaaacttggc tggtttgaaa tagctaattg tccaaattct atcgcggcca
     8221 tctcactcat ttcgtggatc tcaaaaccat atcctcctgg gtcaaacgtt gggggctgat
     8281 tgcacagctc aatgagtttt tctttatatc tctcactgcc accatatttt ccgatcagtc
     8341 ctgaaaaatc gtgataaaca gcaaagctgc tttgccaagc acttccttct gcataatctt
     8401 gtccccaacg attagctgaa aatggttctc tgaacttgcc atctttatct tttgaacgca
     8461 taaatcctga ctcatagtca aagatatttt gatagttcaa tgattgctgc tgatatttct
     8521 tcgtcatttc ctcttcttct agtaccttgg caacttggct aatacaaaaa tcgctataag
     8581 catagtctaa tgtgtgattg actgattcgt ggtagtgatt aggaacatag ccatatgtca
     8641 agtaatcttc tgttccttgg cgtccataat ttggatgttt gctttgaacc gtagcacctt
     8701 tttccattgc cgttagaaat tcaggcatca agtctcgtcg gatatctttt acagcagcat
     8761 ctgcaatgac cgcatcgatc aatgtcccag gcataagtcc acgttcatca ggagacagcc
     8821 attttggcaa atagccactt tctctatagc tgttcaaaaa accttccagc atttcttcgt
     8881 atttttctac agcaatcaaa gaataaagag gataaaccgt tttatacgta tcccaaaacc
     8941 cattatttgt atagagaaca ccaggtttta tagatcgact agtcgtatca tagtggattt
     9001 tttgcccact aggatcaatt tcataaaatg tttgtggaaa taaaaacacc cgatataaat
     9061 tatgataaaa cgtccgcaat tgttgttgat catggtgtgt gagctgtata cgatcaaaat
     9121 aggactgcca ttgttcttga ctgtgagcaa ggtaagtctc tttgctttgg ttttgttcta
     9181 gttccaggtt ataagctgct tgttccgagc tgataaaaga tgtcccaaat cggatagttt
     9241 gtttttttgt ctgttcaaaa tacaacggga tcgagtcgct atttttgttg gaaacagcta
     9301 actcatcaat tgttaaagga tattcaaatc tcaatataaa ataaaaagaa aaatcaggat
     9361 cttctgaacc agcaaaatgt gtgatttttc cttccactgt gtaaggatct ataacaaaga
     9421 gctgatggcg tcccggaaag gataaaagta aaccgctgtc atttttgctg taatcaatag
     9481 ataagattcc gccatacatg ctaggtatca atgtagaaca gatgccataa cgtaattgac
     9541 agatcgacag atgagtcggg caaaatatac tttcttctgg gcggtaagaa ctttgagcat
     9601 ggaaaagggt gttctcctgt agttttccat tgatcggcgt aaacagcata tgactgaaat
     9661 cacccatcca aggacttggt tgatgggtaa gacggtagcc ttgaaaaacg cggtcatcag
     9721 gatgaaacca ccagctgccg ttttgatccg tagtttgcgg tgcaaaataa ttcatcccaa
     9781 aaggaacgcc agtataaggc tggcagttcc cattagagaa atttggctga ttcgctgttg
     9841 catgtctagt atctatttga ccgatattca ttttatttct cctttgttga ttgtcttttg
     9901 atgaatgaaa ctggaaattt actatcggat ggtcgatctt ttgtctctaa ccaagtgacc
     9961 aaagcatctc ccgcaagctt acctaactgt tcaaaatctt gtgcgatggt tgtcagtggc
    10021 gggtcaacaa gagctgctgc ttggatatca tcaaatccga taattgaaaa atcttcaggg
    10081 atatgataac catgctgtct caagagattc atggtctgga tagcgacaac atcgttttca
    10141 caaacaaaag cagttacttc atacttagtg atataagaaa gcaattcatc taatgtcgcg
    10201 cttttgtcat ccagtgaagt gtggaaagtc aaaccagctt tgtccaatgc ttcaaggtaa
    10261 ccaaggtaac gctgtcgaac ggattgtgga tgagtctgcg taccaaacaa ataagcgatt
    10321 tttgaatgtc cctgttcgat caaataagag gcagcaagcg caccgccttg gaaattatcg
    10381 gaaagaacgg taggaaaagg taactcatag atttttttat ctaatatgac gacagggaat
    10441 tctttcaatg aaagctcaaa aagtaagtca agatgttgtt ctgtatcaag agcataataa
    10501 atcaatccgt caaattcatt gattagctct tttgctgttt tattttgtag aaagtcaagc
    10561 gtcgtcatca tgacatcaat ctgtttatct tgcatcacag ggttcaatcc cttgctaaaa
    10621 tcaccaacag acaaatcagc gacatatgga agaagaaaca gaatccgtcg tgtttttgta
    10681 ggagaagcaa gaatctgttc cggagaagcg gatttaacga aactgccttt cccgcgaacg
    10741 cgataaataa gtcctaattg ctctaattca gtcaatgcac gtttggaagt gattctgctt
    10801 actttatagg tacgtgaaag ttctttttca gttggtagtt ggctgtctac aggtaattca
    10861 ccagttaaaa tagccgtttg caaatcatta aatattgtct ggtacaatgg ttgagtcata
    10921 taaacacctc ttaatgatat atcatattca aataatacca taatggatga aaaagtaaat
    10981 aaaaagatat tttttgaaag cgtagacata attggctgta tgaagtataa tctttaaaga
    11041 tatatcaaat agaaagaagg ttttattagt ggtttataaa gatataccaa aatcggtgca
    11101 gaaatttatg gaagatatca cacaaaaatg tggggatatc catgcggact gggcaaagaa
    11161 ttttacagca gcatttgcca atactctgtt gacaacagtc aaaaagcaaa atgacggaac
    11221 caccttttta ttgacgggag acattccagc gatgtggtta agagactcta ctgcacaagt
    11281 tcgtccttat ttagtgattg caaaagaaga tgaggaatta gctgacatga tcagcgggtt
    11341 agtcaaaaag caattttttt atatcaatat cgatccatat gcgaatgcct tcaacgaaga
    11401 agctaacggt gctggccacc aaacagatca tacaaaaatg aacgattgga tctgggaaag
    11461 aaaatatgaa attgattctt tatgctaccc aatacagttg gcctatcttc tttacaaaaa
    11521 tacaggaaaa actgatcaat tcaatcaatc ttttattgaa ggtatcaaaa aaatcttgga
    11581 cgtgttcaaa acagagcaag atcatgaaca ctctccttat acatttgtac gagacacgac
    11641 aagaaaagaa gatacgctga tcaatgacgg gcgaggtgca aaaacagctt ttacaggaat
    11701 gacatggtct ggtttccgtc ctagcgatga tgcttgccgt tatggatatc tggtaccatc
    11761 caatatgttt gcggtggtcg tgttaggtta tgtggaagaa atttttcaaa cgatactcga
    11821 tgataaagaa attgtacagc aaacacagaa attgaagaaa gagatccaag aggggattca
    11881 gaatcacgga cgaacggtta atcaaaaagg gcaaacgatt tatgcatatg aagtagatgg
    11941 aatgggaaac gccacagtca tggatgacag taatgtgccc aatctgatcg ccgcacctta
    12001 cttgggatat tgttctgaac aagaggaaga ttatctgcaa acaagaagaa cattgttgag
    12061 taaagaaaat ccatatttct atgaaggaaa gtacgcaaaa ggaattggat cctcccatac
    12121 gccagaaaaa tacgtttggc cgattgcatt agctatggaa ggaatgacaa cgtcagacaa
    12181 atctgaaaaa gagcggatat tgaatttact tgtagcaact gacgcaggaa cacaccttat
    12241 gcacgaagga ttcgatgtga acaatcctga aaattatacg agagaatggt tttcttgggc
    12301 aaatatgatg ttttgcgaat tagtcatgga ttactttgat attagagtag aaaagtagga
    12361 ggtatcgtaa atgaaaaaga aagtatatat catttctcat agccactggg atcgtgaatg
    12421 gtacatggct tacgaacagc atcacatgcg actgattgaa ttgatagacg atctactaga
    12481 gctatttgaa gttgatccgt ccttcaacag ttttcatttg gatgggcaaa cgattatttt
    12541 agatgactat cttcaagtaa ggccagaaaa aagacaggca atccaacaag caatcaatga
    12601 aggaaaattg agaattggac cattttatat cttgcaagat gattttctta tcagctcgga
    12661 atcgaacgtg agaaatatgc tgattggcat ggaagaaagc cgtaaatggg gaacccctgt
    12721 catgttaggc tattttcccg atacatttgg aaatatggga cagacgcctc aattaatgaa
    12781 acaagctggt atttcagcag cagcgtttgg ccgtggtgta aaaccaattg gctttgataa
    12841 tcaagttttg gaagcagaaa actattcttc ccaatactca gagatgtggt ggaaaggccc
    12901 tgatcaaaca gcaatctttg gtctgctatt tgccaattgg tatagcaacg ggaatgagat
    12961 tccagtagaa aaagaagcag ctcttgcctt ttggaagcaa aaattagcgg atgcagaaca
    13021 atatgcctct acgaaccact tattgatgat gaatggtgtt gatcatcaac cggtacaaaa
    13081 agatatatca aaagcaatcc atctcgcaaa tgaactgttt ccagattacg agtttattca
    13141 ttctaatttt actgactatc tggaagcagt tcaaaaagat gtgccagaag acttaggctc
    13201 agtagaagga gaattgacaa gtcaagaaac ggatggctgg tatactttag ccaatacagc
    13261 atctgctcgg gtttacttaa aacaatggaa taccaaggtc cagcgacagc tagaaaatat
    13321 tacagaacca cttgcgacga tggcttatga agttagtgga aactatcccc atgatcaatt
    13381 agattatgct tggaagacat taatgcaaaa tcatcctcac gacagtattt gcggttgttc
    13441 agtcgattct gtccatcggg aaatgatacc tcgttttgaa aaagcagatg aagttggaaa
    13501 gtatcttgca caagattcct tagaacagct gaccgcagca atcgatacga ccggattccc
    13561 aaaagacagc tttccatttg tcattgtaaa tactgcagga atggataaaa ctggtgaagc
    13621 agaaataacc atcgaattag aaagaaaaag attcgctgaa ggaatacctg aacaactata
    13681 tcaagagtta gaaaatttgc ctaaacgtaa gtatcatgta gaaacaaaaa gtggggccac
    13741 tataccagct attttatctg aagaaactgt ccaatttggt tatgatttgc cgaaagaccg
    13801 tttccgtgtg ccatatatgg caagaatgat caaagtgact ttaccattag aaaacatgcc
    13861 agcattttct tgggaaacat tcgcgcttgt agaaggggaa gcgaaagcag aagaaaagga
    13921 aacaatgatt catcagagtg gacgaatcat cgaaaacggc cctctacatt tgacgattga
    13981 aaaaaatggt accattacaa tggaagatcg caaaaacaaa cgaaagttga ataatctgca
    14041 tatttttgaa gatatcggcg atataggaaa tgagtatatc tttaaacaac ctttctgtga
    14101 tcagccaatc ctatccagta acaaagaaaa ctctgaagta aaagtattag tagatacacc
    14161 ggaaatagca aaaataagta ttctacagga aatggaaatc cctgtttctg cagacgagcg
    14221 tttagaaaaa gaacagcaga tggttgtgga atttcgttat cgaaaagcag aacgatccaa
    14281 agaaaaacga atccttcaaa taaaaacaat catgacaatc agaaaagata gtaaaaaaat
    14341 cgactttgaa acgactctcg acaatcagat gaaagatcat cgcttacgtg tcttgtttcc
    14401 tacaaagtta catgtggaac atcacgaagc agatagtatt tttgaagtgg tgaagcgccc
    14461 aaaccatgtg tctaaaagtt gggaaaaccc cacgaatccg caacatcagc aagcatttgt
    14521 caatattcat gatgaagagt acggtgtaac agtcggaaac tttggtttga atgaatatga
    14581 agtaacagaa gacggacaaa tcgcagtgac gctacttcgc agtgtgggag aactaggcga
    14641 ttggggatat tttccaacac cagaagcaca gtgtctaggt gagcatcggt tcaattacag
    14701 cattgaacta catggaccag aagaaaaatt ctctacttat ttgcatgcat acgcagcaca
    14761 gattcctttt tccactcaac aaatcaaaca tcatgaagga acattgattt caaaacaaca
    14821 atatttaacg ataaaaagtg aaacgtttgc aattacagct ttgaaacgca gcaagtttag
    14881 tgataaagta gtagtaagag gtttcaatat gtctagtcat ttagaaaaat tggaaataac
    14941 taaagacaat ggaaaaactg tcatattgaa tctgcttgaa gaaccaacga agcaagcggt
    15001 tgtcccaata atccaacctt atgagattcg gacaatcgga ttcgaggagg aaaacgaatg
    15061 tatggcggaa tagaagcagg cggaacaaaa tttgtgtgtg cggtttcaga tggtggagaa
    15121 attttagaaa aattaagtat ccctaccaca acaccagaag aaacattgga tcaggtattt
    15181 gaattttttg atcgttatga attgaaggga atcgggattg gctcattcgg cccgattggt
    15241 atcgacaaaa caaaagataa ttacggatac gttttggcaa ctcccaaaaa agggtgggca
    15301 caattcgatt ttcttggcac aatcaaggaa cgctacaata ttcctgctgc ttggacaacg
    15361 gatgtcaatg ctgctgcata tggggaattg aagcaaggag cagcacaagg aaaaaatagt
    15421 tgtatttacc ttaccgtggg aacaggaatc ggtgcaggtg tcgtgatcaa tgaagaaatt
    15481 ttttcaggga tcgcacatcc tgaaatggga catatttggg tgaaacgcca tccagatgac
    15541 cattatgaag gtacttgtcc ttatcataaa gattgtttag aaggattagc agcaggaccg
    15601 tctattgagg cacgcacagg gatcaaaggg caagatctgc cgcaagatca tcctgtttgg
    15661 gctattcagg cttattatat tgctcaagca ctgatcaact atacgttgac gttagcacct
    15721 gaaaagatta ttttaggcgg tggggtaatg aatcaggatc atctccttca aaaaatccgt
    15781 cagcaatttg ttgaattgat gggtggttat atggaaacac ctcaagtaag tgaatatatc
    15841 gttcgttggg gactgccgaa tgaaagtggg attatcggta gtttgctgtt agcagaacac
    15901 gaacatcaaa agtaagcaga caaattacac aaaaagaaca gagtcttatc attatctgct
    15961 gataagactc tgttcttttt tatttctatt cccataatcc aggtaaagct ttaccgacta
    16021 ttttttcttg agtaaatgga tggatcatgt gcagttctaa tgcatggagc atcagtcgtt
    16081 cagctgttct attttggtag agagggtctc caacgatcgg atgtccctta tgagctaaat
    16141 gaactctgat ctggtgtgta cgtccggttt ccaaaacaca atagacttgt gtttgttcag
    16201 aaagtacttt gttgacagtg acatgtgtca aggcgctctg accattttta tcatctacga
    16261 ccctttttct ccgatcatgg cgatctcgtc caattttatt ggtgatggtc atatctttgg
    16321 caatttctcc ccatacgatg gcttgatatc tccgatagat cattttttgt tccaacattc
    16381 ttccaagtat cggcaagaca aatggatttt ttgcaaataa aacggcaccg cttgtttctt
    16441 tgtccaagcg gtggacaacg taaggtcgtt gctgtttagt gtcgagataa gcagctaaat
    16501 gattgagtaa ggtactgttt tctgtcggtt catttggatg agtcttcatt cctgcaggct
    16561 tatccactat aatcaaatgc tcatcttcat ataaaacacg gatgtgcttc gcatctccta
    16621 attgaacttc ttgataatta taatctgttt cttcgaataa caatgtgatc tggtcattgc
    16681 cctttacttc atgatggaac aaagcaggtt gttgattgat ccaaacgttt ttacgtatac
    16741 gcaaaaagtg tcgaaccttt ctaggaacta accattcctt ttcaagcaag tcgcgaattg
    16801 ttgtttcagg atggttcata ggtaaagtga tcgtgatttt cacaagtgta ctcctttcag
    16861 tgcaattgca gactaattgt accattttta aaacatattt aagaaaaaac ttacattttt
    16921 ctgttcattc ttatgttact ctacttgcac taacgtaaaa aaacatattg aactacaact
    16981 taagcaggaa gaaaacactc tttcactatg gtaaaataag acgattgaat caaatagaca
    17041 ggagaagaag caaatggatt ttcaaagcat tttagaaaag ataaaagctg ctttgatcag
    17101 tttttggcgt tggatcagac catatctcgg acaattccat cgatggcgaa agcgcatctg
    17161 gaaaaaatat catgtgaaca aattgatttt gctgattggt ttgatctgtg tcttagtgac
    17221 aagtatctac ctctttattt tagcaaaaca agcaaatgtc gaaacgttaa aatcaggatt
    17281 aagccaatcg acagtcattt atgataagaa aaacgaagaa gctggcacac tgtacgccca
    17341 aaaaggaaca tatgtagaat taaacgcgat ttctccctat atccaagatg cagtaatctc
    17401 gacagaagat aggaattttt acgagcatca cgggtttgat atcaaaggga ttgcccgtgc
    17461 agcagtccga atggtcttgt ctggcggaac atcagggggc ggaggaagta cgatcactca
    17521 gcagcttgcc aaaaatgcgt atctgacatt agaccaaacg tttgatcgaa aagcgaagga
    17581 attatttttg gcaatcgaga tcgaaaagaa gtacagcaaa gaagaaatct tgaccatgta
    17641 tcttaacaat gcctattttg gcaacggggt atggggtgtc caagacgctt ccagaaagta
    17701 ttttggagta gatgcaacga atgttacgct gtctgaggct gctactttgg caggaatgct
    17761 gaaaggtcct ggtatttata atccgatcga tcacccaaaa aatgcgaatg atcggaaaaa
    17821 cacagtacta agtgtaatgg aagaaaacgg aaaaatcaca aaagaacaag aacaagcaga
    17881 aagccagaca gatatcagcg cgtatcttaa tgacacatac gataattcag attctggtta
    17941 tcgttatcct tattattttg atgcagtgat cgatgaagca gttaacgagt atggtcttga
    18001 tgaagaagat gtcatgaata aaggctataa aatctataca gctttagatc aggattatca
    18061 aaagcaatta gaagctactt atcaaaacag tgcattattc ccagcaaatg cttcagatgg
    18121 tgccatggtc caatctgctt cagtagcttt agatccgaat acaggtggtg tacaagcact
    18181 agtaggacga agaggagatc atgtctttag aggattcagc tttgctacac agatgcgtcg
    18241 ttcaccagga tcaacgatca aaccattaag cgtatataca ccagcactgg aagctggata
    18301 caagccttcc agtatcttga aagacgaacc gcaaagttat tacgatgcgc ataattttga
    18361 cgggacatac caaggagaag taccgatgta tcaagctgtt gcacaaagtt tgaatttgcc
    18421 tgctgtatgg cttttgcatg agatcggttt acaaaaagga tatgataaag cagaagaatt
    18481 cggcctgcct ttagcaaaat ctgataagta ttatggctta gcccttggtg gactggaaaa
    18541 aggagtatct ccattgatca tggcgggtgc ttacagtgca tttgccaatg atggtcagat
    18601 gtatacgccg catcttatca cgaaaatcgt tgattcgact ggagcggtga tcgtcgataa
    18661 cacaaacaaa aaaccaaaac aggtcatatc aaaggaaaca gccgatgaaa tgaccagcat
    18721 gcttcttggt acattttcaa atggaacagc ggctgctgcg aatcctgcag gttatacgat
    18781 agcagggaaa acaggaacca cagaaactaa tttcgatgcc acaaaagcaa atgaccaatg
    18841 gatgatcggt tacacgccag acgtagtgat ttcgacatgg atgggttacg aagaatccag
    18901 caagctgcac tatttggaag ggacaagtgg aaatgttgtc gggaaagtct tcaaatcagc
    18961 ggcagaaggc atccttccgt atacttcaaa aacaggattt agtgtagcag atgcctatgc
    19021 cacaggagga caagtggtag ctgcagatca agttggaaac acaggaaatt ctaatggaga
    19081 gtctggaaat tggcaagata acttgaatca atatggtgaa caagcaaaag aagggctgaa
    19141 aaacttcggc gatatggtga aagaaggcgt tcagggaatt ggtgaagcgg ctaaagacct
    19201 ctggcggaaa tatcaaggag agtagcccct ttgaaaacga ataaccccat gttacaatag
    19261 agagtaacta ctaaaagaaa agagggcata acatgtcaaa catctacgat agcgcgaatc
    19321 aaatcgaacg tgagatccgt gaattaccag aattcaaagc gttgaaagaa gcgtacgaga
    19381 aagtacaagc aaacgaagaa gcacacaaac tgttcaaaga cttccaagcg atgcaaatcg
    19441 aattacaaga aaaacaaatg agtggacaag agttcagcga cgaagatgca atgaaagcac
    19501 aagaaatggc tgtgaaaatc caatcagaag aagtaatcaa tgacttgatg caaaaagagc
    19561 aaggcttcag tacaatcatc aatgacttga accgtatcat catgacacca gttcgcgacc
    19621 tttacagcga ataaaactat tttatgaaac aacagagggt gtgaaagaga cgttcggcct
    19681 tgagcgatga caaaattgct tcttattgcc gataggctgt ctcttgaaca ccatttacca
    19741 agaggtcgtg agcctaccgt ttaactctga gttacaagga acaatttcaa aaaacagctt
    19801 ctcatgtttg atgactattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttat
    19861 tcagaaatag gagctacgac aaaagtcttg tcatagctcc tattttttgt cattcatttt
    19921 tcaagcggta gaaatagcct ttttgagcta acagtttttc gtaagtacct atttcaacga
    19981 tttttccttc atcgatcaca acgattttat ctgcctcttt gatcgttgaa agccgatgag
    20041 ccacaataaa agtagtgggt gtttctgtca ggtaattcag ggcttgctgg atttttttct
    20101 ctgactggtt atctaaagct gaggttgctt catctagcaa aatgattttt ggctgacgta
    20161 tcaacgctcg tgcaatggag atacattgct tttggccacc agacagcttg ttgccatgtt
    20221 cgcctactaa ggtatccaga ccatcaggaa gctggtcaat cagtgaagaa agttgcgcag
    20281 cttcgatgac ttcatctaaa cgttcttttg atacgttttt caagccataa gtgatgttct
    20341 ctttgatcga agcggagaac aacaaagtgg tttgaggaac aacggctaaa taatttcgca
    20401 cacttcgcat atccagttca tccaaaggct gatcatctag aaaaattttt cctgaagtcg
    20461 gctggataaa tccaatcaat aagttaagga gtgtagattt tccagagcca gatggcccaa
    20521 caaaagcaat tgtttctcca gctttcacct ctagagaaaa atgatcgata atcggtttga
    20581 gtgagtccgg ataaagaaaa gaaacatcct caaaacgaaa atttccatcc aaatgttgga
    20641 tttttttctt accgtgataa gtttctacat cgccagcaat caacagttcg ccaatagaag
    20701 tgatagattc catgcctttt gaaatcgtag gaatcagcgt gatcattcca gaaacggaat
    20761 tgacgatcgt cgtgaaatac gtctgataca tcacgacatc gccgggttgg atgcgccctt
    20821 gtaaagctaa tatcccagtg aaaatcaaac aaatcagctg aaatatctga aaactagccc
    20881 agctgactga tccgaaaaca gattgcagga tatctaagtg gaatcctttt tcatagattt
    20941 gagtaaaatg cttatttaag cgagttgttt cgtgtttttc taacccatga gctcgagcaa
    21001 tcggtgctaa ttcgatcact tcgatcactt ttgcagaagt ctcttccatt tcttgcctga
    21061 agtctgagtt ggactgtctg atccttttac ggaaaaagag agtaacgaat gcggcgagag
    21121 gaacagtcaa taagaaaaac atgaaaacaa caggactttt ataaagcgtg atacctaaag
    21181 ctacgaataa attgacagcg atatttaatc cgctgacaaa tacttgagag gacaacgttt
    21241 ggatagcttc tacatcacgg atgatctttg actggattcg tccagattgc aattcttttt
    21301 gataaggaat agatagttct tggattttct tgataagggt cgctcttagt ccagcttcta
    21361 cttgacggat cgaagtgcta tggtatttta catgaaaata attcgtcaat atattttgac
    21421 caatcagaca agccatcaaa aaagcgttga aataaatggc ctgcaactga gaagaatctt
    21481 tcgatgaagc tgcattgatt acattggcaa tagcgatcgg cattaaccaa gatggaagat
    21541 gtttgataat aaaaaagagt cctgacatgg caaaacgcca ataatttccc tgataaagat
    21601 gcagcaatgt ctgaaaagca tttgcatgat atttttgata acttttctgt attcgttcct
    21661 ccataatctt ctccttaaca atcttggtaa ataaactgca agtgttctat tgcttcagga
    21721 acataaatct caggagtagc ttcaagagtg gcgtacatta gaggacgctc atatttgata
    21781 taatgcagta ccgggcggaa atctaaacag cccttgccaa caggtaccat gcggatcttt
    21841 ccattttcca tgatatagtc tttgagatga agcgctgtaa tataaccatg taaattgtcc
    21901 aaggcacctc ggatcacttt ttcctgatca gcatgatttt ctttatgcat caaattcgca
    21961 caatctaaaa tgatttttaa atttggtgaa gctacttcat cgattaaacg tttagctaac
    22021 tgatagttat agattggatg attgatccct gcttcaacag caacagttac gccaaaattt
    22081 tctgcatgag cgactaattc aatgatagaa tttctggcga tctgataagc ttcttcagta
    22141 aaattttctt ttgtgtaacc gacagagaca ctaccggttt ccgtagcaat catcttggct
    22201 tgataggcag agcacagagc aatttgctgt ttgaaagttg ccaatgcttg tgcacgaact
    22261 tcttgatttt tgctggatag gttgatatag catcctaaaa tggaaagttc gatattgttt
    22321 ttttggaagt attcaccaaa ataggcagca ctcccaagtg tcatgtcagt taatttaagt
    22381 tgttcaggaa atgctttttg tggtgcaaat tggatatggg acaagtgaag ctgtgatact
    22441 ttttgaacga tttcttcacg atttcgaaca tctaaatcgt gggcacggat gcctaaattt
    22501 aatggaaaca tctcaaactc ctttttgtaa ataattagca agaggcagac gggattcttt
    22561 tatcaattca acaattattc gggcaacttt atcagcgcct tctggactaa agtgggtatt
    22621 gtcttttagt ccttctgggt aatttttaca agtatcaggc ataagatgca ggtagaattt
    22681 ttttgtctct tcttttgaga aggtctggaa caattcttgt gatcttgaga acacatctaa
    22741 aagaggaata tggtgtttct cagcaaatgc acgcatcgct tttggatagt ctccaagtat
    22801 atctggattc aatgttccgt tttctaagta atctcgtcgc gtcacagatg tcagtaagac
    22861 catttggacg ttcgcttttt ttgcgcattc aaaaaatatc tgcagatttt ttaaatagct
    22921 accgaaggga agcgtacctc tttcttcttg gattttctgg tcgttatgtc caaattgtac
    22981 aaataagaaa tcattcggct gaaactgtga agctagacgg gctaaccttc cttcctggat
    23041 aaatgatttg gtgctgcgtc cattctttgc ttggttattg atctggatct cgggtgaaaa
    23101 ataagcagag attttttctc cccaacctgt ctcgggacgc ttgttttcat ctttttctgc
    23161 cgcagtagaa tcaccggcaa tatggatagt aggcatgtta tctcttcttt cataaaggtt
    23221 tataggcaat gaagcagaac agcaagagaa gaagacggag caaat
//
LOCUS       DAZKBH010000032        23201 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000032 DAZKBH010000000
VERSION     DAZKBH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.032"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACG8OO_001229"
     CDS             complement(<1..563)
                     /locus_tag="ACG8OO_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFP9045120.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACG8OO_001230"
     CDS             complement(750..1400)
                     /locus_tag="ACG8OO_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045121.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8OO_001231"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG8OO_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFP9045122.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8OO_001232"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG8OO_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9045123.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8OO_001233"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG8OO_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9045124.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACG8OO_001234"
     CDS             complement(4926..5657)
                     /locus_tag="ACG8OO_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFP9045125.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACG8OO_001235"
     CDS             complement(5661..6926)
                     /locus_tag="ACG8OO_001235"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFP9045126.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8OO_001236"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG8OO_001236"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9045127.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8OO_001237"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG8OO_001237"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9045128.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACG8OO_001238"
     CDS             12268..13770
                     /locus_tag="ACG8OO_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFP9045129.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACG8OO_001239"
     CDS             13772..14509
                     /locus_tag="ACG8OO_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9045130.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="ACG8OO_001240"
     CDS             complement(14554..14727)
                     /locus_tag="ACG8OO_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045131.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14739..15368
                     /locus_tag="ACG8OO_001241"
     CDS             14739..15368
                     /locus_tag="ACG8OO_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045132.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="ACG8OO_001242"
     CDS             complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="ACG8OO_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9045133.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="ACG8OO_001243"
     CDS             complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="ACG8OO_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9045134.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="ACG8OO_001244"
     CDS             complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="ACG8OO_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9045135.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="ACG8OO_001245"
     CDS             complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="ACG8OO_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFP9045136.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20581..21747)
                     /gene="hemW"
                     /locus_tag="ACG8OO_001246"
     CDS             complement(20581..21747)
                     /gene="hemW"
                     /locus_tag="ACG8OO_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP9045137.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21763..21990)
                     /locus_tag="ACG8OO_001247"
     CDS             complement(21763..21990)
                     /locus_tag="ACG8OO_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9045138.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22474..23081
                     /locus_tag="ACG8OO_001248"
                     /pseudo
     CDS             22474..23081
                     /locus_tag="ACG8OO_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
BASE COUNT         6644 a         4974 c         3943 g         7640 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacatttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt ttgttggtac
    15361 aaacatgaca actacaaaaa aaacaggcga acatatgatt gttcgtctgg tttttaatta
    15421 ttaatgactt agcgttttta ctagaaaaac tacttccgct ttttcccgcc aaaagcatct
    15481 ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa ctttgatgcc gccagcttca
    15541 gcgaatgcac gtaatgcttc tctttgttgt tcatttagat ttttcggtgt gatcagtttt
    15601 actttgacat gttggtcgcc tgttgcacca ccacgtaact tcggagcgcc ttttccacgt
    15661 aaacggaagt tcgtacctgt ctgtgttcca gcagggatct tcaatttcac atctccatgg
    15721 acagttggta cttggacttc atcccccaaa gctgcttgta caaaacttaa aggcaattca
    15781 tagtaaattt cagaaccttc acgatcaaag atatcacttt cttctacacg gaagaccaca
    15841 tacaaatcac caaatggtcc gccgttttct ccagcttctc cttgaccagc taaacgcatt
    15901 tgttgtcctt cttctacacc agctggtaca ttcactttaa ctgaatgtgc tttcttctca
    15961 tgtcctgagc catgacaggt tggacaaggt tctttgattt ctttccctgt cccatgacaa
    16021 acatcacaag tttgacggct catcactcgt ccaagtggtg tttggcgttc tacattaatc
    16081 gtacctgcac catgacattt atgacaagta gttggttgtg tgcctggttt tgcaccgttc
    16141 ccaccacaag tatgacagat ttcttcccga ttatacttga tttctttttc tacaccaaaa
    16201 atcgcttctt caaaagacaa gtcgaccgta tattgcaaat cagcaccttg acgaggcgca
    16261 tttggatcga ctgaccgacc gcctcctcca aagaaagagt cgaagatatc ttcaaaacca
    16321 ccgaagccgc caccagagaa gccgccgaat ccaccaaatc cgccaccagc tccaccagca
    16381 ccgtaatttg gatctgtgcc tgcatgcccg tattggtcat atgcagcgcg tttttgcggg
    16441 tcacttaaaa tctcatatgc ttctgatact tctttgaatt tttcttccgc atcaggttct
    16501 ttattgatgt caggatggta ttttttcgaa agcttacgat aagctttctt tatttcatct
    16561 tctgatgctc cttttgagag ccctaagact tcataataat cacgttttgt tgccataggc
    16621 tttcctccta ttggttctca tcattttcac gtcgcttgtt atcatatcat attttgtttg
    16681 aaacaaaaac cgatacttca aactattttt caaaagaaaa agccaaagta gcaactttgg
    16741 ccttctctcg aaattcagtg aactgattat ttatcgtcgc catcgacttc ttcaaaatca
    16801 gcatcaacga catcatctgc tccgccttga gctgcttctg ggttttcttg agcttgttgt
    16861 tgtgccgctt gttcgtatag tttaacagtc aagttttgaa cgatttcgtt caatgcatcg
    16921 cgtttagttt tcatttcttc gatgttgttt gcttcaacag cggctttcaa ttcatcgcga
    16981 gctgtttctg cttttttcac ttcttcttcg tccactttgc cttctaattc tttcaatgtt
    17041 ttgtccacag agaatagcaa tgcatctaca tcattacgta ggtcaacttc ctctttacga
    17101 gctttatctg cttctgcatt tgcttctgca tcttttacca tacgttcgat ttcttcgtct
    17161 gttagacctg aagaagattt gatcgtgatt ttttgttctt tttgtgtacc tagatctttc
    17221 gcagatacat ttacaatccc gtttttatca atatcgaatg ttacttcgat ttgtggaata
    17281 ccacgaggag ctgctggaat atctgtcaat tggaatcttc ctagagtttt gttgtcagca
    17341 gccattgggc gttcaccttg taatacatgg atgtctactg ctggttgatt atctgccgca
    17401 gttgagaata cttgtgattt acttgttgga atcgttgtat tgcggtcaat caatttagtg
    17461 aacactccac ccattgtttc gatacctaat gacaatggtg taacgtcaag taaaacaaca
    17521 tctttcacat caccagtgat cacgccacct tggatagcag cacccatagc aactacttca
    17581 tcagggttaa ctgatttgtt tggttctttt cctgtttctt tacgtactgc ttctacaact
    17641 gcagggatac gagtagatcc accgactaaa ataacttcgt cgatttctga ttgtgataaa
    17701 cctgcatctt ttagcgcttg acgtactggt acttttgtac gttctactaa gtcagctgtt
    17761 aattcatcaa attttgcacg tgtcaatgtc atttccaaat gcaatggtcc agattctcct
    17821 gctgtgataa atggcaagct gatttgtgtg cttgatacgc cagataaatc ttttttcgct
    17881 ttttcagcag cgtctttcaa gcgttgtaaa gccattttgt cttgagacaa gtcaatgctg
    17941 ttttcttttt tgaattctgc taccatgtag tcgatgattt tgttatcaaa gtcgtcccca
    18001 cctagatggt tgtcgccagc tgttgacaat acatcaaaga caccgtctcc tagttcaagg
    18061 atagatacgt caaaagtacc gccaccaagg tcgaatacta agattttttc gtcgcgatct
    18121 gttttatcta aaccataggc aagagcagct gcagttggtt cattgacgat acgttctact
    18181 tctagaccag cgatcttacc agcatctttt gttgcttgac gttgtgcatc attaaagtaa
    18241 gctggcacag tgataactgc tttttcgact ttttcaccta aatagtcttc tgcgaatcct
    18301 ttgatatatt gaaggatcat tgctgagatt tcttgtggtg tatatgattt accttctaca
    18361 tctactttgt aacctgcttc accgatatgg cgtttgattg atgaaatcgt atttgggttt
    18421 gtcacagctt gacgttttgc aacttcccct acttggattt ctccattttt gaatgataca
    18481 acagaaggtg ttgtacggtt tccttctggg ttagcgatga tttttgcttc gtttccttcc
    18541 aatactgcta ctgcagagtt agtcgtacct aagtcaatac cgataatttt actcataatt
    18601 aacgtctcct tctttaattg aaagtgtaag ttttatatcc gcacaattat tgtgcaacaa
    18661 tgaccatggt tggtcttaac acgcgatcat gtaatttgta gccttcttgt agtacttcta
    18721 caatcgtatc ggctggtgta tcttcagtcg ctggaacagt ttgtactgct tgatgaagat
    18781 taggatcaaa ggcttctcct ttagcaggga ttttttcaat tccttcttct tccagtgcgt
    18841 tacgcaaact ttccaagacc atttccacgc cttttttcaa acctgcccct tgatcatctg
    18901 acacttctgt ggctaatgca cgttccaggt tgtcaattga tggaagtaat tttttggcta
    18961 aatcttgtga acggtatttt tgtaattgct cacgctcatt ttttcctcga tttgccatat
    19021 ttgcgatttc agcacgagcc cgcaagtatt tatcttccat ttcttctaac tcagctttta
    19081 atttcgctgt ttcgaattct tccgcttcag cttctgaaat acccgaatct tctgcagctt
    19141 cgacatcgat ttccggttct ggctgagcat ctgtcatttc tttatctagt tcttcttgat
    19201 tttctttcac gatacagtct tcctttcatt attcagtcag ctgtttgatt gctcttacta
    19261 agaaagagag aacgatcagc tgcaaatagc ttcgttattc acattacgag tttgacaagt
    19321 caagcgaacg gtagtaatca gcaagtttag ctgctagttc tcttcgataa acatcgacta
    19381 gaccgaacat ccgagaataa ggcatactag taggccctaa caaggctatt gtacctcttc
    19441 catgaccatc tatctcgtaa cttgcttgaa tcatactcat attctgcaat aaatcattgc
    19501 ctaattcagc accaatcttg atatgaatat tgctatccat cggagtgatc atttgtgtaa
    19561 gtccgtctga gtctttcata aaagaaaaca tcgatttgaa ctgatcaaga tcttgacggg
    19621 gctcataatc caagagattc atttgtccac tgacataaac cttatcttca aaaacatgac
    19681 caagaacggt gtcaaataac tgagacatcc cttctgtcgt ttggaaatat ttatgaagaa
    19741 tcatcggaat ctccgtacga agtttatggt acaccgtcat cagaggatct ccgactagac
    19801 ggtcgtttac aatctgtacc attttttcta gttcctgact tcccatatat tcgggaagag
    19861 tgaacacttg gctttctaca ttccccatat ctgttacgac gatagcaata atctgtcgat
    19921 tattcaaggg tacgatacga aagccagtca gtcggcggtc ttttatttca ggtcccaatg
    19981 aaaaagtcgt atagcttgtc agctcagata atatctcagc agattgctta ataatctcat
    20041 tgatttcatg aaattctctg cctagagact gacgaatcag cagcaaatca tcttctgcta
    20101 cgcgagcagg ttttaacaaa tgatccacgt aataacgata gcctaaagca gatggaatcc
    20161 gcccagaaga agagtgagtc ttcaacagca atccttcatc ttctaatgct ttcatatcat
    20221 tgcgaatcgt tgctgagctg gccttgactc cctcagacat caatgttttg gaaccaacgg
    20281 gcacgccact actcgtatag tgttggatga tcagacgtaa aatgtcgttt tgtcgctttg
    20341 ttaacatctt cctcacctct cattagcact catatattct aagtgctaat ccacttataa
    20401 atatagcaag aagcccttct cctgtcaaga gaaagactcc attttttagc actctaaaca
    20461 caagagtgct aattctaaat aattgatgta aatccagtta tatcaacgtt tcaatagtcc
    20521 tttattgtat ttttgaactt tttttattta aattcagata aaaaacaaat attctttaaa
    20581 ctatttttca aacaaaaagc gctcaaacac attgttcccc aaaaaggtcc ctcttggtgt
    20641 caaataaaca cggtcttctt cattaacaag taacccttct tgctctaatt cccttagcac
    20701 atcaccgtac accgattcaa ttgttttttg atagcgatca tagaaatgtt ttttagtaat
    20761 ccctgttttt tttcttagtc ctaagaacat ttcttcttct atttgatttt ttcgtgtcaa
    20821 tttttcttct tccaataccg ggcgttcatt tgctcgtaat gggcgtaaat agtgttggat
    20881 tggtcctttg ttgcggtaac gaatatctcc aagataaccg ctagcaccag ctccaaaccc
    20941 aaaatagtgg tcattgttcc agtacatgag gttatgttga gattcatgtc cggagagccc
    21001 aaagttactt atttcatatt ggtgacgtcc atgcttttcc attgcttcaa tcgtctcttc
    21061 aaacattctg gtttccgttt cctgatcagg cagttcgagt cttccttgtc gtacccagtt
    21121 catgaacatt gttttgtttt cgaggatcaa tgagtacaaa gaatagtgag gcagatccaa
    21181 agccaaagct ttttccaaag tatccagata tccttctagt gtttgtcccg gtaatgcata
    21241 aatcaaatcg atgcttacat tggtaaaatt ttctgcttct aaaaattgca tagtttgata
    21301 cacatcttct gcggtgtgtt tacgtccaat ttttttcaat aagcgattat caaatgtctg
    21361 cactcccatg gataaacgat tgacgccata gtttttcatt acttgaagtt tttcacgggt
    21421 caaatcacct ggattcgctt ctacagtaaa ctcattacca ttttgaaaag gcaggatctc
    21481 tctagcacct gataacagtc gatcaagttg tttagcagat aaagaagttg gtgtcccgcc
    21541 ccctacgtaa agtgtttcta aattcgctgt cggatgactt tctagcatta ttttcatttc
    21601 attcagcagc atttccacat actcatctac tggctgccct tctaaaaaaa ctttattaaa
    21661 atcacagtaa tagcagatat gctcgcaaaa tgggatatga atgtacgcag aaaccttgtt
    21721 ttctgcggga tttattgttt ctatcattga aattctttct ccttacgtcg aaaagtcgtg
    21781 cctgttttcg accttcttaa aatcgcgttc aagcaccaac tcattgaaat aagccgcata
    21841 tttagaatca aggaaaccta gctctaaaaa tgcttgctta ttgtcatcgg agctacacgg
    21901 tgcttttaca gcctctcgat aaacggtaat cgcaaagctg accttcggca ttaagtcaaa
    21961 atctgcaaaa atatgaaaag ctattttcac agattcctcc ttaagtgctc agaccacaac
    22021 gctttgctta caacctctca tatacgcata ctaaacgtat ttggcgaaca aatccactaa
    22081 aaaatccaag aatcgacttc ttggatgatt ttttctttca ttaaactggt tacctagtta
    22141 tatatctcta gggttgtttt catgtctgag tgcccgatat gatttatttt tcgtttctat
    22201 aattactttt tgccttccag tttcttttaa tattgtactc aagcaagtgg gaattggttc
    22261 ttttgtcatc catgtatgca aaatgttata atgctggtaa acaaaaatga ttacctgtca
    22321 tttttattta gttcgtattt aattaagttg actatctgta tactctgtct aaaacttaag
    22381 aattgttgtt ttcgctgtcg ctacccaatt ttaatgaatg tctgtaggtt aaaactgttg
    22441 tccttgttat agatagtaaa ggagagcttg aaaatgaaaa ctgcgattac gtttctatta
    22501 gaggaatatg ccgattggga aggtgcttat attactagca cactaaacca aaatgagaaa
    22561 tggcaaaaca aaactgcttc tatccaatca gaggtcactt ccattggagg attaaaaaca
    22621 aaaatcgatt acttaatcga acaaatacct gaaaaagttg acctttttat tatgatcggt
    22681 ggcaattcat gggacataaa aaatcatcat ttatatcaaa taatcgataa attgttaaaa
    22741 acagacgtac cagtagctgc tatttgtggg gcagttgatt atttagctca gagcggatta
    22801 ttgcaaggtt acaaacacac cggaaatgcg caatttcctt ggttagaatc ggaaaattat
    22861 cagaataaag aagattttat agaacaacaa gctgtgaaag atcgtaattt gattactgcg
    22921 aatggaacag ctcctataga attcacagac ttgattcttc aagcagtcca ttttgaaaca
    22981 gaagaaataa gagaaaaaac gattcgtcta tacaaacttg gattctatga ttactataaa
    23041 aagtacggag atcctttctc ctaatcattc tcttcaatta aatactcctt atcaatagtg
    23101 tgacaagtaa ttttactgat gattatttgt cacacttttt ctgacattta tcaaagaaga
    23161 tacactctat ttatattcat gagagtataa aatattttgt g
//
LOCUS       DAZKBH010000033        23090 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000033 DAZKBH010000000
VERSION     DAZKBH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.033"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG8OO_001249"
     CDS             complement(54..788)
                     /locus_tag="ACG8OO_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9045139.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG8OO_001250"
     CDS             1115..1525
                     /locus_tag="ACG8OO_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9045140.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACG8OO_001251"
     CDS             1518..2219
                     /locus_tag="ACG8OO_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFP9045141.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACG8OO_001252"
     CDS             2219..3805
                     /locus_tag="ACG8OO_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045142.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACG8OO_001253"
     CDS             3824..4045
                     /locus_tag="ACG8OO_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFP9045143.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACG8OO_001254"
     CDS             4042..4803
                     /locus_tag="ACG8OO_001254"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFP9045144.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACG8OO_001255"
     CDS             5183..5692
                     /locus_tag="ACG8OO_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9045145.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACG8OO_001256"
     CDS             complement(5767..6306)
                     /locus_tag="ACG8OO_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFP9045146.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8OO_001257"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8OO_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9045147.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACG8OO_001258"
     CDS             7319..8170
                     /locus_tag="ACG8OO_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9045148.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACG8OO_001259"
     CDS             8198..8833
                     /locus_tag="ACG8OO_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFP9045149.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACG8OO_001260"
     CDS             8853..9626
                     /locus_tag="ACG8OO_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9045150.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACG8OO_001261"
     CDS             10034..10831
                     /locus_tag="ACG8OO_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFP9045151.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACG8OO_001262"
     CDS             10809..11222
                     /locus_tag="ACG8OO_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9045152.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8OO_001263"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8OO_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9045153.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8OO_001264"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8OO_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9045154.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACG8OO_001265"
     CDS             15999..16559
                     /locus_tag="ACG8OO_001265"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFP9045155.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8OO_001266"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8OO_001266"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFP9045156.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACG8OO_001267"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACG8OO_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFP9045157.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8OO_001268"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8OO_001268"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9045158.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG8OO_001269"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG8OO_001269"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9045159.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG8OO_001270"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG8OO_001270"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9045160.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACG8OO_001271"
     CDS             21906..22484
                     /locus_tag="ACG8OO_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9045161.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACG8OO_001272"
     CDS             22536..23018
                     /locus_tag="ACG8OO_001272"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFP9045162.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
BASE COUNT         7523 a         4177 c         4950 g         6440 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa
//
LOCUS       DAZKBH010000034        22051 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000034 DAZKBH010000000
VERSION     DAZKBH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.034"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG8OO_001273"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG8OO_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFP9045163.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG8OO_001274"
     CDS             complement(2020..2922)
                     /locus_tag="ACG8OO_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9045164.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG8OO_001275"
     CDS             complement(2919..3611)
                     /locus_tag="ACG8OO_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFP9045165.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG8OO_001276"
     CDS             complement(3654..4295)
                     /locus_tag="ACG8OO_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFP9045166.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4611)
                     /locus_tag="ACG8OO_001277"
     CDS             complement(4333..4611)
                     /locus_tag="ACG8OO_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045167.1"
                     /translation="MRFVSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFI
                     FLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4638..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8OO_001278"
                     /pseudo
     CDS             complement(4638..5264)
                     /gene="pyrE"
                     /locus_tag="ACG8OO_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8OO_001279"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG8OO_001279"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9045168.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACG8OO_001280"
     CDS             complement(5984..6916)
                     /locus_tag="ACG8OO_001280"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFP9045169.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACG8OO_001281"
     CDS             complement(6913..7689)
                     /locus_tag="ACG8OO_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFP9045170.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8OO_001282"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG8OO_001282"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9045171.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACG8OO_001283"
     CDS             complement(10933..12009)
                     /locus_tag="ACG8OO_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFP9045172.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACG8OO_001284"
     CDS             complement(12014..13294)
                     /locus_tag="ACG8OO_001284"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9045173.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACG8OO_001285"
     CDS             complement(13313..14239)
                     /locus_tag="ACG8OO_001285"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFP9045174.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACG8OO_001286"
     CDS             complement(14252..15535)
                     /locus_tag="ACG8OO_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFP9045175.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8OO_001287"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG8OO_001287"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFP9045176.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACG8OO_001288"
     CDS             complement(16323..17234)
                     /locus_tag="ACG8OO_001288"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9045177.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8OO_001289"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG8OO_001289"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9045178.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACG8OO_001290"
     CDS             complement(17700..18398)
                     /locus_tag="ACG8OO_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFP9045179.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACG8OO_001291"
     CDS             complement(18385..18771)
                     /locus_tag="ACG8OO_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045180.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACG8OO_001292"
     CDS             complement(18918..20588)
                     /locus_tag="ACG8OO_001292"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFP9045181.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACG8OO_001293"
     CDS             20771..21004
                     /locus_tag="ACG8OO_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9045182.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACG8OO_001294"
     CDS             complement(21103..21534)
                     /locus_tag="ACG8OO_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFP9045183.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACG8OO_001295"
     CDS             21613..22026
                     /locus_tag="ACG8OO_001295"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFP9045184.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
BASE COUNT         6238 a         4927 c         3892 g         6994 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttcaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt t
//
LOCUS       DAZKBH010000035        22028 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000035 DAZKBH010000000
VERSION     DAZKBH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.035"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1371)
                     /locus_tag="ACG8OO_001296"
     CDS             complement(61..1371)
                     /locus_tag="ACG8OO_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFP9045185.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ACG8OO_001297"
     CDS             complement(1383..2801)
                     /locus_tag="ACG8OO_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9045186.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="ACG8OO_001298"
     CDS             complement(3041..4609)
                     /locus_tag="ACG8OO_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFP9045187.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="ACG8OO_001299"
     CDS             complement(4613..4894)
                     /locus_tag="ACG8OO_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFP9045188.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="ACG8OO_001300"
     CDS             complement(4891..5814)
                     /locus_tag="ACG8OO_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFP9045189.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="ACG8OO_001301"
     CDS             complement(5811..6689)
                     /locus_tag="ACG8OO_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFP9045190.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="ACG8OO_001302"
     CDS             complement(6676..8013)
                     /locus_tag="ACG8OO_001302"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFP9045191.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="ACG8OO_001303"
     CDS             complement(8016..9119)
                     /locus_tag="ACG8OO_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9045192.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="ACG8OO_001304"
     CDS             complement(9193..10701)
                     /locus_tag="ACG8OO_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9045193.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="ACG8OO_001305"
     CDS             complement(10667..11359)
                     /locus_tag="ACG8OO_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP9045194.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="ACG8OO_001306"
     CDS             complement(11381..12355)
                     /locus_tag="ACG8OO_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9045195.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="ACG8OO_001307"
     CDS             12655..13527
                     /locus_tag="ACG8OO_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9045196.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="ACG8OO_001308"
     CDS             complement(14109..16307)
                     /locus_tag="ACG8OO_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045197.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="ACG8OO_001309"
     CDS             16539..17531
                     /locus_tag="ACG8OO_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9045198.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="ACG8OO_001310"
     CDS             17591..19012
                     /locus_tag="ACG8OO_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9045199.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="ACG8OO_001311"
     CDS             19005..20504
                     /locus_tag="ACG8OO_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFP9045200.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="ACG8OO_001312"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="ACG8OO_001312"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9045201.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaat
//
LOCUS       DAZKBH010000036        21988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000036 DAZKBH010000000
VERSION     DAZKBH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.036"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG8OO_001313"
     CDS             <1..395
                     /locus_tag="ACG8OO_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFP9045202.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACG8OO_001314"
     CDS             398..1147
                     /locus_tag="ACG8OO_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045203.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACG8OO_001315"
     CDS             1159..1428
                     /locus_tag="ACG8OO_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045204.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACG8OO_001316"
     CDS             1433..1597
                     /locus_tag="ACG8OO_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045205.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACG8OO_001317"
     CDS             1590..1892
                     /locus_tag="ACG8OO_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045206.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACG8OO_001318"
     CDS             1889..2050
                     /locus_tag="ACG8OO_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9045207.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACG8OO_001319"
     CDS             2047..2352
                     /locus_tag="ACG8OO_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045208.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ACG8OO_001320"
     CDS             2352..2708
                     /locus_tag="ACG8OO_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFP9045209.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="ACG8OO_001321"
     CDS             2668..2913
                     /locus_tag="ACG8OO_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045210.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ACG8OO_001322"
     CDS             2910..3329
                     /locus_tag="ACG8OO_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFP9045211.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ACG8OO_001323"
     CDS             3326..3883
                     /locus_tag="ACG8OO_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFP9045212.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ACG8OO_001324"
     CDS             3880..4176
                     /locus_tag="ACG8OO_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045213.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ACG8OO_001325"
     CDS             4253..4666
                     /locus_tag="ACG8OO_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9045214.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ACG8OO_001326"
     CDS             5012..5386
                     /locus_tag="ACG8OO_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045215.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ACG8OO_001327"
     CDS             5423..5590
                     /locus_tag="ACG8OO_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045216.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ACG8OO_001328"
     CDS             5616..5960
                     /locus_tag="ACG8OO_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP9045217.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ACG8OO_001329"
     CDS             5965..6246
                     /locus_tag="ACG8OO_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045218.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ACG8OO_001330"
     CDS             6349..6663
                     /locus_tag="ACG8OO_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFP9045219.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ACG8OO_001331"
     CDS             6641..8335
                     /locus_tag="ACG8OO_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9045220.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ACG8OO_001332"
     CDS             8355..9533
                     /locus_tag="ACG8OO_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9045221.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ACG8OO_001333"
     CDS             9496..10182
                     /locus_tag="ACG8OO_001333"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP9045222.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ACG8OO_001334"
     CDS             10182..11342
                     /locus_tag="ACG8OO_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9045223.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ACG8OO_001335"
     CDS             11352..12227
                     /locus_tag="ACG8OO_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045224.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ACG8OO_001336"
     CDS             12224..12535
                     /locus_tag="ACG8OO_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045225.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ACG8OO_001337"
     CDS             12525..12878
                     /locus_tag="ACG8OO_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9045226.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ACG8OO_001338"
     CDS             12868..13269
                     /locus_tag="ACG8OO_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045227.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ACG8OO_001339"
     CDS             13262..13666
                     /locus_tag="ACG8OO_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045228.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ACG8OO_001340"
     CDS             13678..14286
                     /locus_tag="ACG8OO_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9045229.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ACG8OO_001341"
     CDS             14306..14668
                     /locus_tag="ACG8OO_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045230.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ACG8OO_001342"
     CDS             14671..14853
                     /locus_tag="ACG8OO_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045231.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ACG8OO_001343"
     CDS             14870..18289
                     /locus_tag="ACG8OO_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9045232.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ACG8OO_001344"
     CDS             18340..19077
                     /locus_tag="ACG8OO_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9045233.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ACG8OO_001345"
     CDS             19087..21378
                     /locus_tag="ACG8OO_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFP9045234.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ACG8OO_001346"
     CDS             21402..>21988
                     /locus_tag="ACG8OO_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFP9045235.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAZKBH010000037        21983 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000037 DAZKBH010000000
VERSION     DAZKBH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.037"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(324..1442)
                     /locus_tag="ACG8OO_001347"
     CDS             complement(324..1442)
                     /locus_tag="ACG8OO_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045236.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1675..1797
                     /locus_tag="ACG8OO_001348"
     CDS             1675..1797
                     /locus_tag="ACG8OO_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045237.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1913..3220
                     /locus_tag="ACG8OO_001349"
     CDS             1913..3220
                     /locus_tag="ACG8OO_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFP9045238.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3341..4540
                     /locus_tag="ACG8OO_001350"
     CDS             3341..4540
                     /locus_tag="ACG8OO_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9045239.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4567..5835)
                     /locus_tag="ACG8OO_001351"
     CDS             complement(4567..5835)
                     /locus_tag="ACG8OO_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFP9045240.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(5891..5957)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6005..6646)
                     /locus_tag="ACG8OO_001352"
     CDS             complement(6005..6646)
                     /locus_tag="ACG8OO_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFP9045241.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(7055..7558)
                     /locus_tag="ACG8OO_001353"
     CDS             complement(7055..7558)
                     /locus_tag="ACG8OO_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9045242.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7681..8445)
                     /locus_tag="ACG8OO_001354"
     CDS             complement(7681..8445)
                     /locus_tag="ACG8OO_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFP9045243.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8553..8828
                     /locus_tag="ACG8OO_001355"
     CDS             8553..8828
                     /locus_tag="ACG8OO_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9045244.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9388..10338
                     /gene="yidC"
                     /locus_tag="ACG8OO_001356"
     CDS             9388..10338
                     /gene="yidC"
                     /locus_tag="ACG8OO_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9045245.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10384..10785)
                     /locus_tag="ACG8OO_001357"
     CDS             complement(10384..10785)
                     /locus_tag="ACG8OO_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9045246.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(10799..11608)
                     /locus_tag="ACG8OO_001358"
     CDS             complement(10799..11608)
                     /locus_tag="ACG8OO_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9045247.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(11595..12485)
                     /locus_tag="ACG8OO_001359"
     CDS             complement(11595..12485)
                     /locus_tag="ACG8OO_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9045248.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(12498..12977)
                     /locus_tag="ACG8OO_001360"
                     /pseudo
     CDS             complement(12498..12977)
                     /locus_tag="ACG8OO_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(13153..14094)
                     /locus_tag="ACG8OO_001361"
     CDS             complement(13153..14094)
                     /locus_tag="ACG8OO_001361"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFP9045249.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(14091..15086)
                     /gene="lacD"
                     /locus_tag="ACG8OO_001362"
     CDS             complement(14091..15086)
                     /gene="lacD"
                     /locus_tag="ACG8OO_001362"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFP9045250.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(15079..16275)
                     /locus_tag="ACG8OO_001363"
     CDS             complement(15079..16275)
                     /locus_tag="ACG8OO_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9045251.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(16293..17021)
                     /locus_tag="ACG8OO_001364"
     CDS             complement(16293..17021)
                     /locus_tag="ACG8OO_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9045252.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(17462..18436)
                     /gene="lacD"
                     /locus_tag="ACG8OO_001365"
     CDS             complement(17462..18436)
                     /gene="lacD"
                     /locus_tag="ACG8OO_001365"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFP9045253.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(18433..18891)
                     /locus_tag="ACG8OO_001366"
     CDS             complement(18433..18891)
                     /locus_tag="ACG8OO_001366"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFP9045254.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(18888..20351)
                     /locus_tag="ACG8OO_001367"
     CDS             complement(18888..20351)
                     /locus_tag="ACG8OO_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9045255.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(20333..21265)
                     /gene="pfkB"
                     /locus_tag="ACG8OO_001368"
     CDS             complement(20333..21265)
                     /gene="pfkB"
                     /locus_tag="ACG8OO_001368"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9045256.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            21407..>21983
                     /locus_tag="ACG8OO_001369"
     CDS             21407..>21983
                     /locus_tag="ACG8OO_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9045257.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         6598 a         4739 c         3688 g         6958 t
ORIGIN      
        1 aaatttcaca aaaatttgag aaataatttt tgtcaatctc ttcttatttc ttgaagctga
       61 ccacttctgt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
      121 gcccaccaaa caaaaaatat gaagagctat ttttgcttgc tgttcttatt acttgatgca
      181 gaacagcttt ttcacaacct ctcatacacg gtgtttaatc agctgacctt cggtattagc
      241 tcaaaaaacg gcaaaatatg tgaaactatt tttccgtttg tttctctaat actcaggtca
      301 aaacactgat ttcacaacct cttttaataa cgaagaatcg ctgtcagact cttttcatct
      361 ggcgcatctt tttgatccaa aataaacact tttccgccat taatgatcac atcatctgct
      421 aatgcattca acacttgacg ccgatcatat tctgtttcag gatcttcacc gaaaccatca
      481 gctaagtttg acgtagcgat aaataaatgt gacacttttc cttctttcgc agctggaacg
      541 atatcaacta actgatcgat gaatttctta tctaagagtt tattgtattc ttttgattct
      601 tgagctgcga gttcacttga cagcttcttc gccccttctt cgatatctct aaaggatagc
      661 tgcgcaggag aagcagaaat cgcaacgtct gcacaataat agggattttt agcaattttt
      721 ttgaacatcg tctgattttc tggtagagca aataaataaa tcggccattg ttctggattt
      781 tcgaaagaat ccttcaagta cgtatcaact gcttggtaat agttgaccca atcgatctct
      841 acttcttcat cttttggatt tacgccatgg aaagttactc catctcgggc tccgcctcct
      901 ccattagaag agaagttcaa accgccacct gtcagttcag atcccaatgc agtaacgaca
      961 tcaaccggtg ccccttctgg tagagatact tcttttactt gtttgttgct gacttcatac
     1021 aatttcatgg agtcgcggtt caatcccaat aaataataag aatagttgaa ctgcccattt
     1081 ttgataatag ccagtaaata aggaagatca cttacatagt attgatcgtc taccgggata
     1141 ctcaaacgat ggatataggt ttggtcagca gttataatga ccgctacact cgtagttgca
     1201 ctacgccaaa acgaaggatc tcctaacaaa ttttcaattt tttcttggaa cggggaccag
     1261 tcatgttcgg aatatttttt ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat
     1321 ttgatctgat ctttttccac atcttgatgg gcgacatgcg tattcagcat gatcgtgacg
     1381 aatggtcctt tagcgccttc tgaagtcaat tgtgacaata attctttatc cttttttgcc
     1441 ataaacgtac ccctcctaaa aaaatcattc ctttgtttct atctaaagtc taccacaaca
     1501 ctatttttcc atacaataaa acgtatctaa aaaaataaaa cggtttctac ggttttaaat
     1561 ttagctattt ttttaagtta tcttcataat ttcctcaatt atttagcccc agtcttcaac
     1621 tattcttcat gaaatgctga taaactaaga aaaagaataa aagaggtgga tcttatgcat
     1681 ttgattatga ttttcagttt attaagtatt atcagtttat ttgctttcag cgcttcccgc
     1741 ttattagatg cggaaacaca gcctattcct gtaaaagtac gtaaaaacga ccgctaacct
     1801 tccataacgt gcgttcttat cctaaagttt tttcagaaac attgaaactc tctcccctct
     1861 ctgataaaat tttaatttgt agcgaaacaa attaattaca aggagatcgg atatgtccat
     1921 aaaaaaaaca cgtctagtcc tattattggc tagtctcatt ctttgccttg cttcttttct
     1981 accagctgtt gctgtgcaag ctgaagatac tttcaaagta aatgcaaaag cagcctttgc
     2041 tgtagatgcg caaagcggta agattcttta tgatcaagat ggagaaaaaa ccatggggat
     2101 cgcttcaatc acaaaaatca tcggtcttta catcgtcctt gatcaagtaa aagaagggaa
     2161 actatcatgg gatgataaag tttcaatttc agattatgca gaaaatttga gtgtcacccc
     2221 agatttatca aatgttcctt tgcataaaga aaacacctac accgttaaag aattattcga
     2281 ctctgcaatc atccagtcag ccaacgcttc aatggttgcc ttggcagaaa aaatctctgg
     2341 cagcgaagca aaattcactg aacgaatgaa agaacaattg aaagattggg gtatcaaaga
     2401 tgcaacgatc gttaatgcat caggactgaa taactcttat cttggcgaaa accgaccaga
     2461 aggtactgga gaaaacgacg aaaatcaaat gtctgctcaa gatgtggcta tcgttgcccg
     2521 tcatttgatc ttggattttc cagaaatact ggatgtatct agcactacga cacaaatgtt
     2581 tggcgaaaac acgcaatcac cagtagaaat ggtcaactgg aactggatgc tcccaggatt
     2641 cgtgaattat aaagaaggcg tggacggatt aaaaactggt acgactgaac ttgcaggagc
     2701 ctgtttcgtc ggtacgatca ctaaaaacga tcaacgaatc atcactgtcg tattgaatgc
     2761 agacggccat gcggaaaacc caagtgcccg tttcaatgaa acagctcgct taatggatta
     2821 ttgctacgac aattggtcag aaaaagagct tggaaaagcc aatgcaagta tccctagtct
     2881 aaaaaccatt gatgtaaaag acggaaaaga atcaacagtg cccgttgtgc ttaaaagtcc
     2941 tgtaaaagta tgggtacgga acgatatgga tactggaaaa ttaacaatca caccaactct
     3001 tgacaaaaaa caagtcactg acaatgagat ccaagcacct atggataaag gaacaaaaat
     3061 cggtaccgca acgatcacct tggcagaaga taaattaggt tatcttgaag acaacaacgc
     3121 gccatccaca gaaatcatca cggataaaac cgtagaaaaa gcaaatattt tcgttattgg
     3181 ctggcgacgc gtcgcagaat ttttcggtaa cttgttttaa ttgaacaaaa agaaaaaagc
     3241 atctttccat gtggaagatg tttttttgct ttttctagtt taaataacag cttttttcat
     3301 ctattttcgt tatactgtat aagaaaaagg agttgaattt atgaatctaa ccagagatct
     3361 gatttttttg gttctctatc ttattggggt attctccatc gtttattggc gaaaacagaa
     3421 aaaggatcct tccttgttct tttacagcag tacatggacg tttgcggtaa tcttgactta
     3481 ttttgtcgtg ttggaaattt tttattcgac cttttatttt ttgattccct tagtgttttt
     3541 cagtatcttt gccttttcct atttttcaga aaagcgaagg ctgttgaatg gtcttttatt
     3601 caatattttc ctcattagtt ttggtatcta cttgtttgtc ttgctatacg aaacacaaaa
     3661 tatttttctt ggcggactga ttgccttgat cacgatcccg ttactgcttg tctttctatt
     3721 cggcatttat ggattgatcg tttttctttt ttggaacggt gtcacagttt tacgtagaga
     3781 gtcgcattca ttagctaatc ttttaacgtt gatactagcc atttttctta cattgtttct
     3841 tgtttttgac tttttcttgt taaaatattt accacaatgg ataaatgctt tgtttttctg
     3901 catcccattg atcttgatct atctatttat cgtattctac aactttttga ctgtctcatt
     3961 cttgtatcag ttcaaccgcc ctcgatacaa ccaagacttc atcgttgtgt taggcgccgg
     4021 gttgatcaat ggagaaacag tttctcctct tcttgccaag cgaatcaata aggcgattgc
     4081 tttttattgg gcacaaagcc gtgcgacatt aaatccgcct atcctattaa tgtcgggtgg
     4141 acagggagca gacgaaaaag taccagaagc aatggcgatg aaacaatatg ccatggaaca
     4201 aggaattcct gaaagagata ttcttgtcga aaccaattcg acaaccactt tggagaatat
     4261 gcgctattca aaggaaatca tggatcagca aatgaaaggt ccctatcgcg cgatcttcag
     4321 ttccaacaat taccacattt tcagagctgg tctctatgca cgacaagcga aactgaaggc
     4381 aaatggcatt ggggcaaaaa cagctttcta ttacttgcct aacgcgtttt taagagaata
     4441 catcgccatc ttgatgatga acaaaaaaag acatatgatc atttgcggat tgcttttcgc
     4501 aggttccgca ttcttatcac tgatcacttt acttttctaa aaaaacaagg tgtgacaaaa
     4561 catatgtcac accttgtttt tttcttttat tctcttgacg attggcaaga atagtattcc
     4621 ttttttgacg gctacttgct gtgcagaata aataccgctg tttcctgaac acataaagct
     4681 gatcaagcag atcataaaga agtaagcagc agcttgtgaa ccaaataatt cgattcccat
     4741 cacaaaacag gcaatcggtg tatttgtcgc accagaaaat accccgataa aacctaatcc
     4801 agcaagaaaa ggaacagaaa catggagcaa aggcgctagt actgcgccaa gcgttgcacc
     4861 aatctcaaat agtggtgtca cttctccccc ttggtacccc gcacctaaag acaagaccgt
     4921 gaagaataat ttcccaataa aatcataagc atgtgcctgt ccattaaatg attcctgcag
     4981 taacggaaga ctcaacccaa ggtaacgttg tgtttgcaaa atcaacgctg cggctacaac
     5041 tactgctcca cctatcgtat ttcgccaaac aggatgagga atccattgcg cataccgcgc
     5101 ttttaaaaag acgatcgaac ggctgaatac ccaaccaatc aaaccaaaag caatcccagc
     5161 aacaaaaact ttgaagaaca gcaacatact ccattcagga atcacaccca tttggtaatg
     5221 tagatgagaa acaccatagc tttctgtcat gaaatttgca aaaagcgcag caaagaagct
     5281 aggaaatatt gcttctgttc ggactttacc gattgccaac acttctaatc caaataatgt
     5341 tccagctaaa ggtgtgccaa atacagaact aaaacccgca ctgattccac tgataaccaa
     5401 aatctcccgt tctgctttat ccaatcggaa taaatgcgct atattatcag ctaatgctcc
     5461 ccccatttga actgctgttc cttcacgacc aacagatccc ccaaataaat gggtagtgat
     5521 ggtcccaaac aaggtcaatg gaatgagccg caaagggatc ttctcttctc ctccattccc
     5581 ttgatcgatc actaagttat tcccacgact agaaagaccg ccatgatacg cataaagata
     5641 agcaaaaagc gcgccgctga ctggcaatag ataaagtagc caaggatgtg ctagtcggat
     5701 atctgtcacg aacgtcaggc tatttaagaa aaaagcagac aagctcccca tgaatactcc
     5761 gagaatcgct gtgaccacca gccacttcaa catgtacatc aaaatacgca ctggactcgg
     5821 taattgtttt tccataaaaa attcctcctc aaataaaaaa acgcctacta aagaaagggg
     5881 ctggccccag actttagtag gcgtcattag ctatcttacg ttagcattta aaggcgaaca
     5941 ccatcaccta ttcacttcca ttaaaagata gcatattttt ctgtaaaaaa caataaaatt
     6001 atcgttattt ctttacacgt tcagcaaatt catagaaagg agccaatttc gttaactgtt
     6061 gattacattg atcactatca tgacaaatat aattcccctt tttcgtatac gtgccatcag
     6121 aaccggcttt cgttgtagaa agaaagagcg agacattcga tgttttctga cagatcgcac
     6181 agattccctt attaatcgtt ggtgataaag taccgctgat cccttttaat tttccatctt
     6241 cgtaataaac catgaatttt ttttgcgtcc ctggatcatt ccaaccgatg tacgtatgtg
     6301 cttgcaagtc gatttcattc caagcgggtt gtttgagttt tttcactttt cggaataatt
     6361 tctcgatttg tggtttgctc ggctgcataa acggctggac gatttgcttc agctcctcca
     6421 atgctcgttc tgcttgcgtt tttgtcagtt ttacatctaa aactgtcgtt aaaaacgtat
     6481 cgatttctga tggttcgtaa cctaatacat cgcgaatctt ttcagctgct agagcctgaa
     6541 ccgtttgtac cgtttttgag tcattgacag atgagtacgc atttaataac tgctctactt
     6601 ctctttttat aaaaaaatat tgatatggtt tcattgtttg ttccatgatt ttaaagtcct
     6661 ttcttctaac caaagtgcat actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta
     6721 cctgaagatc ccggactctt ttaaataaga tacgttttgc atcttatcat cacccccttt
     6781 aatgttcatt cttctatgta catgattcat tcgataaata aaagtaaacc gtatcttgag
     6841 cagatagtca actgcaaaca gaaaaaggac tgtgacaaaa gtcgtgtcaa agtccctttc
     6901 tacaaacaaa cggtgttcca gaagtagctt cttcaaaaaa taagccggaa tttcacaaaa
     6961 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgaccc acttctgtca
     7021 caacctcaat atggtctatt attggtttta cctgttaaat cgaggattct tttccgaagt
     7081 aacgttgcca tctcttacgc atacttgctg acataggctg agcggcttct ttgactgcgc
     7141 agtatttgtc tacgaatgtt acgatatatc cttctttgta cttaggcggt gcaatcgttg
     7201 cgccccacat gtgcttcaaa atgatatctc gctcaagatc cgacaaatcg gtgatctttt
     7261 ctgcattctt tacagcgatt cttggatgga tatacgcatg tgtgccttca tcaaatttcg
     7321 tcgtacgcca atcatagtag aacagatcat gcaaaagtcc agcacgcgct gttgctttag
     7381 cattgccacc ccattttttt gcgagcagat aactatagta agaaacactg atggaatgct
     7441 ccaaacgagt ggaatgaaca tgctgtgtgt aattcgctaa ttttttcact gcttctgttt
     7501 ctaaaagatc ttctacataa gacatatatt cttcgtcttc acgccatttt tctgtcataa
     7561 ttatcgatct gccacctttt tgaatttcat tttcttctga aattgatcta agtataccac
     7621 cagtgttcgg attattctag tcgccttgga taaaatagcc aaagcttaat agttccttaa
     7681 tcataaagaa aaatgataaa gaacaatccc tgccgcaacc ccaacattca acgattccgc
     7741 atttcctttc atcgggatat atacgttctg atcagtcaaa gacaagattt cctgacttac
     7801 cccctgacct tcgttcccca tgattattgc gaagttatct gatttttcta ctttgtctaa
     7861 ggcgtgtgct tcttcattta attctgtgcc atacactgta aaccctttgt ctttcattaa
     7921 aggaatcact tctgtcaaat ctttttgcaa cacaggcaaa tgataattgc ttccctgcat
     7981 gctgcgcagc acctttgtac tataaatatc tgccgttcct ttgcctaata aaacaccagc
     8041 catatcaaat gcatctgccg tacggatcat cgtccctaca tttccaggat cttgtacatt
     8101 atctaataac agccatcctc ctgaaaaatc tggcgtgcct tcagccgtta acgccatcac
     8161 tgctaagatt ccttgtggtg taggtagatc cgatacagca tccatgacct catccgaaac
     8221 tagaaaagta gaaatctctt tgtttgcggc cagccagtct ccccattctt tcatgcctcg
     8281 ctgtgtcaca aaaagttctt ttatttctgc atcagacgac gcagcttctt caatcagatg
     8341 gaacccttcc aaaagatatt cttccttttc ttcccgatat ttctttttat gtagtttttt
     8401 taattctttg attcttggat tttttgctga ttggatctct ttcacattcc tcacctcccc
     8461 atgattatat catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt
     8521 aatttataaa ataggaaaga aaggtgacga acatgcgcaa agttaaaatg aacgtccaag
     8581 gacgcgtcca aggcgtagga tttcgctaca tgaccaagat ggtcgctgat caactaggcg
     8641 tgactggaac ggtaaaaaac gaagatgacg gctcagtcac gatcgaagca atcggcaatg
     8701 acgacatcat ccagaaattt attgaagaag tcaagaaatc tccgagccca agtggacgag
     8761 tgcagtatgt ggatgtccaa gaagacccga tgattgagga acgaaagaaa tttgatgtgg
     8821 tgggataagg ttgtgaaaaa gctggtctgc atcaagcatt aaggacaggt tgtgaaaaat
     8881 agctctccat atttttcaac aggctggact taatgccgta gatgcaagct tttgaacacc
     8941 gtcaataagg ttgtgagaaa gtcgcttagt tttgagtagt aaggagaatt ttgtgaaaat
     9001 ggctttccac atttttgcat aaaatttgac ttaataccga gaaactgact tttgaacacc
     9061 gtcgataagg ttgtttttga gacgctttgc ctcaagtatt aaagataggt agtaaaaaag
     9121 ctgatctata acttgcaata aatttgctta acccatattt tccagacaga acaaaaaagt
     9181 ttttagtttt ttaaataaga aagaaacact tggaatcact cttttctttc caagtatttc
     9241 tttcttgatt ttttgtcttt ttttcaaaat ttttcgctac gtaaaagaac gaatatttat
     9301 atgaaatttc tatgaagttg attgaattcc atgcgctttt ttagtatagt taagtttgtg
     9361 taaaaattat agaagaggaa gtattctatg aataaatgga aaaactggtt attgggatcc
     9421 ggacttgtca gtatcctgct ctttttatct ggctgtgtgc ggatggattc aaacggaaac
     9481 cctgacactt cgggaatcgt ttatcgagta ctcgtccatc ctatgggcca agcgattaca
     9541 tatctagttg agaatttcaa ttggtcttat ggctgggcag ttattgcaat gactgtgatc
     9601 gtccgtatca tcatcttgcc actaggtatt agtcaatcga aaaaaacaat gatccaatct
     9661 gaaaaaatgc aagcgttaaa accacaagtt gaagcagccc aacaaaaatt gaaagctgca
     9721 acaacacgtg aagaacaaat ggcagcacaa gcagaaatgc agcaagtata ccgcgaaaac
     9781 ggattgagta tgaccggcgg aatcggctgt ctgcctctat tgatccaaat gccgatcttc
     9841 tctgccttgt actttacggc acgttataca gaaggtattc gggaatcatc tttctacggc
     9901 atcgatttag gctcaccaag tatggtactt gttgtcattg caggggtcgc ctatctgctt
     9961 caaggataca tctcaacgat cggtattcct gaagaacaga aaaagacgat gcgtacgatg
    10021 ctaatcgtct ctcctgctat gatcgtcttc atgtcgatca gtgcgccagc cggtgttaca
    10081 ctttactggg tagtcggtgg tgtgttcagc tgtctgcaaa cattcatcac aaacgttatg
    10141 atgaaaccac gtttgaaagc acaagtggca gaagagttga aacaaaaccc gccaaaacaa
    10201 gtcgtcactc caagaaaaga cgtgacagaa tcagcaaagc cagctaacaa caaacaagca
    10261 acaaaacaaa tcaaacaacc aaaaaacagt acaaacaaca aaggccgcaa cgcaggaaag
    10321 cagcagaagc ggaaataaaa acataggaca aaaaagatga gagctaagcc ctcatctttt
    10381 ttgttactct tcctcttctg ctattccatt aattaattcg ttgacagaga caatgctttc
    10441 acatcctgca cgaacaaact cgggctctga accaatcatt tgtgagttga taaattcaat
    10501 gaccatcgga agattcatcc cagcataaag agtaatctgt tgtccttcca ttattagttt
    10561 agaaatgata tttgcaggtg taccacctaa aagatcgcat agtaccacaa aatcatttaa
    10621 cgaatctgtg acagtgagaa acttttgttt gtatgtttca gtgccatctt ctggtaataa
    10681 aggtaacgtg tagatatcct cttgaggccc catgatcatt tctgtacttt ttttcaattc
    10741 ctcacaaaat tttccgtgac taatcaatac caattgtcta ctcatttttc gctctccctt
    10801 atttcgaaat cacacccaat gcagataaag caacacacac aattaatacg ataaaaatcg
    10861 ctcgagtagg agtcatgttt ttctttccta ataaccaata aacaccaccg acgatcccag
    10921 caggtaaaat tttaggtaag atcatatcga tatagttttg catattcaat gtcacatcgc
    10981 caatcgttgg tgcccatgaa aatttaacat tgatcattgt cgcaactaat gcccctacca
    11041 tgaatacccc taataaagtg gctgcgtccg ttaatgcagt taagcgagat tgcatagtag
    11101 taactaagga aattccttct ttgtatgcaa attcaagttg cttccaacga aaaaccatga
    11161 caacgatctg agcaaaaatc catataaaca cacctatcgg atttccatca gttgccatat
    11221 tcgccgccaa agccccaaaa atcgttggaa tcaaagcagc aaatacggaa tctccaatcg
    11281 ctgcaaagga acccatcaat ccagctttca gccccgatac ggtttccttt gaagcaattc
    11341 cctctttttc ttccattgca agatcaatcc cagtaatgat tgtattaaaa aaattagaag
    11401 tattaaaaaa ttgcgtatgt gttttcatca tctcttttaa ttctggtgta ttgtctccat
    11461 atatttttct tagttgtggc aagatagtat ataaatagcc agaaccctgc attctctcat
    11521 agttccaacc taattgaaaa gtgaacaaac ttcttcgatt gatttgctta aaatcttctt
    11581 ttgttaattt gtaattagat ttcgtcatct tcgatttccc cctcaacaga atgtgatggc
    11641 tcactagcta atggttgatg gtctttgttt tgatcattct tataactgat cattgctagt
    11701 gctagaccaa tcaatgctac agcaagcatc ggcagcgaat tgaaagttgc cggaagatct
    11761 ttaacaacgc ttgccacact tgttcctaat aattgaacat tagagaataa cgttgctaat
    11821 aatgccgtga tcgtaaatcc taaaataaga taagatagat gtttttttac tggtaaatag
    11881 cgcagaagaa tggcaaaccc aacagctggt aaaacagctc ctgctactga aagtccatcg
    11941 cctagccatt ttagattccc gtttaaaaca gaaacaacat tttcaactaa tccaccacca
    12001 aatgctaaag ctaaaaatac aggaatcata cgagagagcg accaaggtaa agctcccatt
    12061 aagaaattgc gttcgattgc ttgataattc atgtcttcta cgtgacggtc aatacgatgg
    12121 gcaaagtatg tgttagcgaa tctagctaaa atatccaatt ggatcattaa gcttgctaca
    12181 ggcactgcaa tagcagcaat cgcttgttct ggattcattc caagagaaat cgagaaggca
    12241 gttgctaaaa tcgtcccaga gttggcatct atttttgatg cgcctccaaa cgtccccact
    12301 cctaaaactg tcaactgcat actgccacca ataatcaaac ctgcgtttaa atcacccatt
    12361 actaaacctg catataaccc tgcaaacact ggcgcactca ttgaagaata gatctgtaat
    12421 tcatctaaaa tttgataacc tgcatataat gttaataata agatctgcca ccattgaata
    12481 tccatggatc attcctccta tttgattaaa agattcataa agtcttctgc tttatcgttt
    12541 ggtaccattt gagcaattaa atgtatacca agctgatcta attttttaaa tacttcgata
    12601 tctgcatcta ccacattgat tgattttgta atggaccgtg tttcattcgt ttgtgacata
    12661 ttccccacat ttacttcttt gataggaact cctttttcta ctaaagataa gattcgatct
    12721 ggcttttttg ctacgatcaa tactcgttga gagtcatatt tgccagctaa aatatttgtt
    12781 gctgcttttt cgattggcaa aatgcttaat tttacccctg ctggagtcgc taacttcaat
    12841 ccactttttt caattgtgtt tgtggctacc tcatcatcca cgaccatgat tcgactaata
    12901 ttcagtttcg ttgtccaaag atttgctact tatccatgta tcaaacgtcc atcgattctt
    12961 acaccaataa ttgccatcat gcattctcct tatctatttt ttctatttat tgttcaatta
    13021 aaactgtgac atcatcaaat atcccttctg tttcaaagat aacgttttca ttttttcttt
    13081 cttatatttc atttttaagc taagaacaaa ttccttccca actaaagaaa acgctttctt
    13141 ttatatttaa gttcatttga ttttcttaac ttgaatgtgt ttcatcaaca tcgtaacttt
    13201 catagggtcg atccagccag ttttctcttg ttgcgcgttt gccataccac aagccataga
    13261 aaatgcgaga atctcttcga ttgattgcct ttttgataac gcatgggccg atcctgcaag
    13321 ggtcgcgtca cctgaaccaa caggattaac aactgatatt ttaggaataa tgacttcgta
    13381 aaaacactga gcaacttttg ccacagcacc tctctctcct aaagaaacaa caacccatgg
    13441 cactccttca aatattggat gagctaataa ggactgaatt ttttctaatt gaatcgaaga
    13501 acagatacgt tggccaatca gttctcctaa ttcttccaga ttgggcttga taagtagtgg
    13561 cttctcctta ccttccaata cttcaagcaa aaatttacca gaagtatcta gtaatatgtc
    13621 tttccctttt ttatcagcta aagcaaccaa ttgcttatag taatcaagag ggcatccttg
    13681 gggtaaacta cctgagatcg tcataatatc tgatatttcg aggagctgct cgaaatgatc
    13741 ggtaaatgtc tgttgttctt tctttgttat gaaggggcct cgttccaaaa gctctgtttg
    13801 tctcgaacta tgcaggatag caatagaatc ccttgtctct cctttaattg gaacaaactg
    13861 atgcatgatc cctaactgat ctagctggtt ctttagatat tcaccgtgct ggccacctat
    13921 taaaccagta gctatcacct cttctcctaa taaatttaga accctcgcca cattcactcc
    13981 ttttcctccc gctgttttat tgactgtttc aactcgattg actttgtcga ggtttaattc
    14041 ttcaattgta tacgcgatat cgatggaagg attcaaggta actgtcgtaa tcatttcatc
    14101 ttcacctttt tgctttttgt tttattgaac caaggggtag cggtttcatt tattacttta
    14161 tttaaagtct cgatgttttc tctcccattt gttcttaacc aatgaatagc tccgtctttt
    14221 ccattttcga cgtatgcttt gaccccatct ttccatgtcg cacgtccaca caatacacca
    14281 ttgaaggtgg aacccgattc atgagcaaaa atcagtgttt gttgaaataa ttctgttgaa
    14341 acacccccac tcaaaaaaat gaatggtaat ggagtcgctt cactttgctc cttaaagaag
    14401 gcagcagcct cttctttgct gtgaataaat tcacctgtgg aatagccatt cacataattc
    14461 atattgactg gtacttctac tttcaacaca tcaactttat attttggatc agaaaattct
    14521 ttcatcatct caataacttt gtggggcttg actttcgcat actctggaga agaactatcg
    14581 cttatttgtg catcataaga aactaactcc aaatagaagg gacgattttc tccttcgcac
    14641 tcactaccta aacgttcgat gaacacatgc ttcaaatgat tgatttcggg tgcttcatca
    14701 acatcgtagt aaagtaagaa ttttactgca tccgcttgct gttctttcaa tctgagagaa
    14761 gaccaatccg ctaataaatc aggtagacga ccaggcgtag ttgcgtcata gccggttttc
    14821 tcataagcta acaataaccc cgtctctttt gctctgactt ttgctgctgg caatccatat
    14881 tccggatcca ataaaatagc agaagcatag ggtgtcagct cagctgatac tagttctttg
    14941 aattcactga tttggtcttc acttggctct tttcccaacg atttgatcat tttttttaga
    15001 gcaccacgct gatcgatcgc taatgcacta ataatcccat cttttgttga tagtctctcc
    15061 attgccctat ttttttcttc attcatctgt catttctcct tcggtttcat acttatgaat
    15121 aatgacgccc ttgactactc gattgaccgt gcctgttgga gatggtgtat ctggtttatt
    15181 agctactttg attgaagtat agagtgcaat gatttgtgca gtcataatca tcgctaatgc
    15241 aagataggca tcgggcaatt tactcaccga gtcttccaat agcacagtca tacctgagaa
    15301 atcaatagta cccggttgcg caatcgcaat gacattggca gtgacccgat cttcttttat
    15361 ttcctctaaa atatctaagt cataatttcg cgtatatggt tgattgctca caaaacctac
    15421 aacgagtgtc tcgctattta aaaaagactt tggtccatga cgaaacccca tcgaagaatc
    15481 aaaaattgta gcaactttac ctgctgttaa ttctaaaatt tttaattgtg cttctcttgt
    15541 cgcacctgcc aaacttcctg aacccagata agcgatccgt tcaaaaaaat caggtaataa
    15601 cttttgaatt tcttgatctc ttgaaagaag atcttgaccg attttaacta attgttcaag
    15661 atatctttct tttttctcca ttgctgtttg atcaaagatc cataaagcac tcaataacat
    15721 acacgtaaaa cttcctgtca tagcaaaacc tgcatcgttt gctctctctg gcatcaataa
    15781 cagataattc tgtttatcat cctgtgcgtt ttttgccaac atcccctctt tagcacaagt
    15841 aatcgtcaaa tgaaacaatg tttcaacttg ttcatttgca atttgcacag ccgctacact
    15901 ttctggactg ttgccactgc gagcaaatga aactaataaa gtcggttcat ttggaaacaa
    15961 aaattctttt ggtgaagcta caatatctgt cgtgccaaaa ctgtggaaga taaaaaactg
    16021 attgtctcca tgttcattta gatagggtaa gagcgtatcc ccaatatatt gagacgttcc
    16081 tgcacccgta aaaataacac gtattcttct tccgtttgct tcttttttta tttgatcaaa
    16141 aaatagagct aaattttctt ggctttgcat caatctatcg attgtttctt tccagagatc
    16201 tggttgttgt tttatttctt gtgtcgtgat tgctgcacct atttgtttta atttgttctt
    16261 cgatagctca aacatgttgt tctccctctc aatcattttc ggatatgccg aattttataa
    16321 tgaaactggt ccgctcttgc tacactatct gtatattcga taatctcatt tttcacgtta
    16381 taggttatcc tcgtcagatg taagacggga gcgccatcag cgattcgtag cacaacagca
    16441 tctttttttt gcacgatact tgcataaaac tcttcatcag ctaattgaac ttgttgttca
    16501 taatcctgtg aaaacacatc atacaacggt ttactttcaa gtaacggcaa atcaagtccc
    16561 ataaatagtt tcgccggtag ataagaacgc tcaaccatca atggctcttc atctgccaaa
    16621 cgcaatcttt taagtttaaa tacagtttca cctaaatgga tgttcaaatt cttagcaatt
    16681 ctcttattag cttccatttg tctaaattct aaaatgaccg tcttagggac gcgccctatt
    16741 tcgcgcattt cttctgtaaa gctatatgct ccagctaagt tcgatgactt atcagacaaa
    16801 tcagagacat aggtaccttt tccatgtctt cggtaaatat aaccttgatt ttccaattcc
    16861 tgtaaagcca gacgtacggt ggtacgactc accccatagt gttgacaaag ttctctttca
    16921 gataacagct tatcatgcgg agtcatctgt gtttcgatac gatccttcaa taaatcaact
    16981 agttgataat acaatgattt tcctttattt gagctctcca taaaaatcac ctcttcttta
    17041 ctggttataa ccacaacaca agaatagtga attattaatc atttgtcaaa ggttttttag
    17101 accaaaaaag ctattataac agctctttaa agctttatat cttgaataga caaagatacc
    17161 tcaaaaatac cagttaccaa taaaagaaca gaaaaaaaga cgaaccacat aagcgatccg
    17221 tcaaaatggt ctaacttatc gagcagcgtg atccacatta atccctatga tattcctaca
    17281 actatcgttt tattacttcc aatatcattt acaacaacta aaaatagctt attaatgaca
    17341 aatgttatta ttagttttag ataacttttg cagttttata ctttttttct ataaaatttg
    17401 acaaacattt ttttaaataa ctaattattt tttgaatctg tcactcagac aatcctttct
    17461 cttacttgtt aacataaata gaagttgctg tttcttctaa tagtgtactt aattctttaa
    17521 tattttgttt tccttcttct aacatccaac tactcgcttt ttcttcgcct tcggtaacaa
    17581 aaggtgctac tcccttcgcc catgttgctc gcccacataa gacaccattg aatgtcgaac
    17641 ctgattgttt tgcaaaacgc aatgtatctt gaaatagtgc agcgctgact cccgcactta
    17701 aaaaaataaa cggttgttca gtcactttcg attgttcata gaaatacgcg gatgcctctg
    17761 cttgactata acaagtatct tgcccatatc cttccacaaa tctcatattc actggtactt
    17821 ctactttcaa tacatccact tgataacgtt cttttgaaaa ttctcgcatg gcttcaatca
    17881 ctttatgcgg ttttactttt gcatagtcaa gagaagacgg atcgccaata gttgcatcgt
    17941 aagaaaggag ctctaaaaag aaaggtatgc cttctgcttc gcattctgaa ccaatccgtt
    18001 ctataactat ttgtttttgc acattaatct ctactggttc atcaacgtca tagtacaata
    18061 aaaatttaca agcatccgca cccacttctt ttaacctttt cactgaccaa ctattcagta
    18121 aatctggtag tcggcctggc attttcgcat catagccggt tttttcataa gctagcaaca
    18181 aaccacattc tttctctcga agcgctgctg caggcagacc atattctgga tcgagtaaaa
    18241 tagcggaagc atacggagtc aggtactgag aaaccatctg tttaaattgg atgatatctt
    18301 ctgatgtagc ttctttatat tggttgatca ttttcttcaa agcaccacgt tggtcgatag
    18361 ctagcgcgct aataattcca gcttcatccg tcaatcgctt caaacgttcc tgtttttcta
    18421 tactcatttt cttcatatat cttcacctat ttctttagtt aatattgtag ctatttctat
    18481 ttttgatttt gcagctctca actgttctgt aacctctttt cgcatcaatg atcgtgcaac
    18541 gagagacaat aatttcaaat gagtcgttcc tgcttctgaa tctgggataa ataacgcaaa
    18601 aataaaatca actgactgtc tatctaaact gttccattca attccttttt tcagtgtgac
    18661 gataatcaaa ctaggttcat tgattgtttg agacttcgca tgagggatag caaaacctga
    18721 cattattcct gtagtacctt ctttttctct ttccagaagt ttttgatata cttctttttc
    18781 gtcatgagcg attcctaaac agaatgtttg ctttgctaaa aacttaaatg cttcttcttg
    18841 attatctaat ggctgttgta aaaaaatgtt ctcttcatga atgattgtca tgataaacct
    18901 cctgctcttc cttcttcgat aactgtacga tttgttgctt tattatccct cttcttgtct
    18961 ttatcagtta ctcttttctt ttggatcaaa cctagtatca ctccaccaac gatcgaacca
    19021 ataagaatcg ctgtaaccca ttggattttt cccgtcacta caggtaacac taaaaaaccg
    19081 ccatgtgggg ctggtacttg gacaccaaac agataggtca agacagcagc aattgatgag
    19141 cctaacatca tcgttggtaa tactcggatc gggtctttcg cagcaaacgg aatagctcct
    19201 tccgtaatat gagttgatcc taaaataaaa ttaaccaaac ctgcattacg atcattagta
    19261 tcaaaatatt taccaaacaa taaagtagca aaaccagtaa tcaacggtgg tgcaatgcaa
    19321 gctgctgacg caccagccat aaaagtcgtg tttccttgtg tcaaaagagc tgttcctgtt
    19381 acatatgccg ccttattaac cggtcctccc atatcaaacg cacacataca gccaacaatc
    19441 acgccaagaa caagtggatt cgaatattca aatccagcta aaaagctcat cattccttca
    19501 tttatagcct tcattggacc tgccagcaaa gacatcacga tgccaataaa tgctacacca
    19561 ataactggat ataagacgat tgccttcaag ccatctagtt gtttaggtaa aattatcagt
    19621 agcttttgca aaaataagat aaaataacct gctaagaacc cagctaaaat acctcctaaa
    19681 aatccagtac cgccattcat ggctacgatt ccaccaacaa atccaacgac taaccctgga
    19741 cgtttcgcga tcccttctgc aatataagct gctaaaactg gaaccatcat tcccattgct
    19801 gcatcgtcaa ctgcttttac cattgccgct gtagcattgt attgtgcatg atctggatca
    19861 gaagaataga ttccccaaac ggcaaatgaa atagcaatca atactccgcc agctaccacg
    19921 aaaggcaaca tatgagagac accgttcatc aaatttttat agatgctacg accaaaatta
    19981 gcaccactta tcgcttctgg ttctaaagta tcagcagctt cattatctgc taagacagta
    20041 ccttttcctg ccaatgcttc gtcaatcagc cgttgtgcat ccttgattcc tgtactgaca
    20101 gaaacatcaa tgactcgttt tcccacaaat ctttctgaat ggacatcttt gtctgcggca
    20161 ataatgacag catctgcctg tttgatttct tcagccgtta attcattctc gatcccaatt
    20221 tgtccatggg tctccacttt gatcgtgatg ccttttttcc tagctgcttg ctctaatgcc
    20281 tcttgcgcca tgtaggtgtg cgcaattcct gtcggacaac cggttgctgc aattagttga
    20341 tattttccca tctttcgaac ctctcttcct tgattttgat ttgtttactt aattcctcca
    20401 catccaaaaa atcagtcagt cctttacgaa aagcagtaga gctgcctgct gctatgcttt
    20461 tacgtaacgt ttcatcaagc gatcgtcgat tcatgtatcc tgctaaaaaa gtgcctaaca
    20521 tggcatcacc tgcacatgct gtattgacaa cttttccttt tggcgagttt cctcgaatca
    20581 ccctgtcttt cgtaaaaaga atcgctcctt tctcaccgag ggataacaaa acattttctg
    20641 cgcctgactc tactagtcgt tgtccataaa caaagtaatc ttcatctgta accatttttc
    20701 gtccgaacca agaagaaagt tcttcctcat ttggtttcag caaaaacggt cgatagggta
    20761 aacaatccat gacttcttga tcactactat caataatcaa acaaatccga ctgatttcta
    20821 ttaaaacctt tggagacagc cctcttggta aactcccaga aacacaaaga taatcccctg
    20881 cttgtaatgc tcttatttga tttaaaaaat tatgaacagc tgtctgcggt atttcaggtc
    20941 cttgattcac taatttgtat tcttctcccg tttgattgac ttgtgtaaat acattgatac
    21001 gcgtcatacc aggcacttca ataaactctg tttggatttg tttctgctgt aaatagtctt
    21061 ctatatactt tcctgtgaac ccagcagaaa aacctaatgc cgtacttggg aaatctagca
    21121 tctttaatac tagtgacaca ttcacccctt ttccgtttgc ctgaatgtca tcctcttttg
    21181 tgcggtttac cataaaagga tgcatctcat ctgtttcaat aaatagatca attgccaaat
    21241 tcatcgtgca tgtgtagata gacataacca acgacctcct tgattctatt atgaaatacg
    21301 cgaaatagaa aatgttttct taatcgtccc aacacaaaga aaacctttac atttataaac
    21361 tcgtcgtgct ttttttaact aacttttggt gaaagaagga aagaatatga atcacaaatt
    21421 aagtgaaaca gagcagtacc tctggaactt tatcgaacac ctcatcctag agatccctaa
    21481 ctattccatc gtgaaactaa gtgaacaagc aaatgtctca acagccacca ttgtgcggac
    21541 aatgaaaaag aaaggctatg aagggtttac ttcgtttaag catcatctaa aagaaggaga
    21601 aaacaagaac atcaattttt catttttaga taaagtagac aaaggaatcc gccgagccat
    21661 tctaaaaaat gaacaagaag tggtacgtac aatcaatatg ttggaaatcg ggaacattga
    21721 agatgcaata caaagaatca aatttgctga ccgggttttt gtctttgccc gaggattttc
    21781 agaaatgatt ggccaagaaa tgctggtaaa acttcaatta acgggaaaaa attgtcagat
    21841 gcatactgat ccagaaatta tcaaaagcat cagtcggaaa ctaactaaga aagacagcgt
    21901 tatttttgtt tctttgaacg gtgagaaaac aaataaaaac atcaaaaata agatttatgt
    21961 ctttattttt caatttttta aat
//
LOCUS       DAZKBH010000038        21912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000038 DAZKBH010000000
VERSION     DAZKBH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.038"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8OO_001370"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8OO_001370"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9045258.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACG8OO_001371"
     CDS             complement(1172..1867)
                     /locus_tag="ACG8OO_001371"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFP9045259.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACG8OO_001372"
     CDS             complement(1880..2596)
                     /locus_tag="ACG8OO_001372"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFP9045260.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACG8OO_001373"
     CDS             2926..3534
                     /locus_tag="ACG8OO_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFP9045261.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACG8OO_001374"
     CDS             3859..4182
                     /locus_tag="ACG8OO_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFP9045262.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACG8OO_001375"
     CDS             4364..5734
                     /locus_tag="ACG8OO_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFP9045263.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACG8OO_001376"
     CDS             complement(5864..7165)
                     /locus_tag="ACG8OO_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9045264.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACG8OO_001377"
     CDS             complement(7519..9510)
                     /locus_tag="ACG8OO_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFP9045265.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACG8OO_001378"
     CDS             complement(9654..9920)
                     /locus_tag="ACG8OO_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9045266.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG8OO_001379"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG8OO_001379"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9045267.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG8OO_001380"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG8OO_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9045268.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACG8OO_001381"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACG8OO_001381"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9045269.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACG8OO_001382"
     CDS             complement(14080..14601)
                     /locus_tag="ACG8OO_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFP9045270.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACG8OO_001383"
     CDS             complement(14604..15446)
                     /locus_tag="ACG8OO_001383"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFP9045271.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACG8OO_001384"
     CDS             complement(15439..16164)
                     /locus_tag="ACG8OO_001384"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFP9045272.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACG8OO_001385"
     CDS             16340..16636
                     /locus_tag="ACG8OO_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045273.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACG8OO_001386"
     CDS             17204..18796
                     /locus_tag="ACG8OO_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9045274.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACG8OO_001387"
     CDS             18809..19549
                     /locus_tag="ACG8OO_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9045275.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ACG8OO_001388"
     CDS             19717..21066
                     /locus_tag="ACG8OO_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9045276.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ACG8OO_001389"
     CDS             complement(21194..21784)
                     /locus_tag="ACG8OO_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9045277.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DAZKBH010000039        21364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000039 DAZKBH010000000
VERSION     DAZKBH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.039"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..746
                     /locus_tag="ACG8OO_001390"
     CDS             45..746
                     /locus_tag="ACG8OO_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9045278.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1355..2371)
                     /gene="tsaD"
                     /locus_tag="ACG8OO_001391"
     CDS             complement(1355..2371)
                     /gene="tsaD"
                     /locus_tag="ACG8OO_001391"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9045279.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2368..2832)
                     /gene="rimI"
                     /locus_tag="ACG8OO_001392"
     CDS             complement(2368..2832)
                     /gene="rimI"
                     /locus_tag="ACG8OO_001392"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9045280.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2839..3381)
                     /gene="rimI"
                     /locus_tag="ACG8OO_001393"
     CDS             complement(2839..3381)
                     /gene="rimI"
                     /locus_tag="ACG8OO_001393"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9045281.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3365..4087)
                     /gene="tsaB"
                     /locus_tag="ACG8OO_001394"
     CDS             complement(3365..4087)
                     /gene="tsaB"
                     /locus_tag="ACG8OO_001394"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9045282.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4278..5258)
                     /locus_tag="ACG8OO_001395"
     CDS             complement(4278..5258)
                     /locus_tag="ACG8OO_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9045283.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5282..6766)
                     /gene="galT"
                     /locus_tag="ACG8OO_001396"
     CDS             complement(5282..6766)
                     /gene="galT"
                     /locus_tag="ACG8OO_001396"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFP9045284.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6778..7767)
                     /gene="galE"
                     /locus_tag="ACG8OO_001397"
     CDS             complement(6778..7767)
                     /gene="galE"
                     /locus_tag="ACG8OO_001397"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9045285.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8015..8182
                     /locus_tag="ACG8OO_001398"
     CDS             8015..8182
                     /locus_tag="ACG8OO_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFP9045286.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8244..10055)
                     /locus_tag="ACG8OO_001399"
     CDS             complement(8244..10055)
                     /locus_tag="ACG8OO_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFP9045287.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10052..10417)
                     /locus_tag="ACG8OO_001400"
     CDS             complement(10052..10417)
                     /locus_tag="ACG8OO_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9045288.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10580..10975)
                     /locus_tag="ACG8OO_001401"
     CDS             complement(10580..10975)
                     /locus_tag="ACG8OO_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9045289.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10993..11955)
                     /locus_tag="ACG8OO_001402"
     CDS             complement(10993..11955)
                     /locus_tag="ACG8OO_001402"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFP9045290.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11955..12167)
                     /locus_tag="ACG8OO_001403"
     CDS             complement(11955..12167)
                     /locus_tag="ACG8OO_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFP9045291.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12188..12886)
                     /locus_tag="ACG8OO_001404"
     CDS             complement(12188..12886)
                     /locus_tag="ACG8OO_001404"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP9045292.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12906..13448)
                     /locus_tag="ACG8OO_001405"
     CDS             complement(12906..13448)
                     /locus_tag="ACG8OO_001405"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9045293.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13580..14584)
                     /locus_tag="ACG8OO_001406"
     CDS             complement(13580..14584)
                     /locus_tag="ACG8OO_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9045294.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14581..15570)
                     /locus_tag="ACG8OO_001407"
     CDS             complement(14581..15570)
                     /locus_tag="ACG8OO_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9045295.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15567..16373)
                     /locus_tag="ACG8OO_001408"
     CDS             complement(15567..16373)
                     /locus_tag="ACG8OO_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9045296.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16539..17495
                     /locus_tag="ACG8OO_001409"
     CDS             16539..17495
                     /locus_tag="ACG8OO_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9045297.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17572..18090)
                     /locus_tag="ACG8OO_001410"
     CDS             complement(17572..18090)
                     /locus_tag="ACG8OO_001410"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9045298.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18178..18327)
                     /gene="rpmG"
                     /locus_tag="ACG8OO_001411"
     CDS             complement(18178..18327)
                     /gene="rpmG"
                     /locus_tag="ACG8OO_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9045299.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18555..19001
                     /locus_tag="ACG8OO_001412"
     CDS             18555..19001
                     /locus_tag="ACG8OO_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFP9045300.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19196..21091)
                     /gene="asnB"
                     /locus_tag="ACG8OO_001413"
     CDS             complement(19196..21091)
                     /gene="asnB"
                     /locus_tag="ACG8OO_001413"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFP9045301.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6420 a         4544 c         3692 g         6708 t
ORIGIN      
        1 aaagtgacaa taaatatagt gaacaataag gagaggactt tttaatgcgt gttaataaaa
       61 tcgattttca cttttcaaaa ctcagatatc aacctgattt tgaacatttc tcggatgaag
      121 agttccaaca gatcaaggaa catactgttc ttcgttctta taaaaaagga cagatccttt
      181 tcgacgatgg tgatcccaga caacggttgt atatcttgac caaaggactc gttcgtttag
      241 aacgttatga tgaaagtgca acttattact actatgacta tgtgaataca aataaccttt
      301 ttcctatgtc aggaatgttt ggagaagaga cctacactta tacagctcaa gcgatgactg
      361 atattgaaac attctacctt cctgtttcct actatgaaaa attagcacga aaaaatcccg
      421 aacagttgat ctatatgata caaaaaattt ctaaagtagt tgaaatacat gaattgcgta
      481 tccaaatcgg attgacctct agcgcctttg accgggtaaa acaaaattta ttcattttga
      541 aagaagaatt gggcgtacca acaaaagatg gtcatatcac cattccttat ccgatcacct
      601 taaaagaatt agcagtgaat agcggcacga cacgtgaaac tgctggacaa gtaatcaaac
      661 aactacgtga ttccgggctg ttagattatg acaaaaagcg ttttactttc tgtaagacag
      721 ctactactcc agctatgaga gaataacgcg aacaaaaata agcagtgacc ctgataaata
      781 tcagggtcac tgcttatttt cctccctaaa acttctgaaa gatccggcag ttttagatca
      841 ctggacaaga ctcaaagatt ttctatgtgt gtttcagtta tgtaatattc actgtttgca
      901 aaaaatactg ttagattaac tagatgaagc gtcttgtcct catccgatct agattattcg
      961 atattcctcg acaataaacg attactcagt gagggcaatc agaggaacgg ataatgataa
     1021 ataagctgta aaaaatagca aaataacaat aatgattaaa agtatgtaaa aaatagggct
     1081 ttgacaaaaa cgtgtcacag ccctatttcg attaaacggt gtgtacctgt caactcttcg
     1141 gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt tccttgtgaa
     1201 tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa gcttgcatac
     1261 gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg gctgttctta
     1321 ttacttgatg cagggcagct ttttcacaac ctctttattc agataaaacc aagcttggat
     1381 cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt gctgctgctc
     1441 caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt tctacctcag
     1501 ggagtttagc tgccaaagca tcctgcaatc cttcacgcaa cccttgattg gccgctacac
     1561 ctccagctat aatcagttgc ttgactggat aattttgaca agctcgaagt gttttagtaa
     1621 tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg tctagatctt
     1681 ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat ccgctaaaac
     1741 tgaaatcata attatcttca tggatcatcg ctcgcggaaa atggtaatta tcttttccct
     1801 gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat actcttccaa
     1861 ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt tcatatgatc
     1921 catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt gccatcaatg
     1981 gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata tggtttacag
     2041 gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata ccaatcagca
     2101 acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct tctgccgaca
     2161 caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg tgatggcggc
     2221 tagctacttc aggtactacg ccaccaaaac gtttatgact gtttatttga gaagcaacga
     2281 tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca tcacaacttg
     2341 attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta actttctgct
     2401 cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac ctaatatacg
     2461 aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta cttctaaaaa
     2521 aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat gccgtgcatg
     2581 cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt catctattac
     2641 tttcgagtat ccaataaagc cacagatctg gttatcttca gctaaaagca gataatcaga
     2701 gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac catgtggata
     2761 agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa aatctttttt
     2821 tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca acgcatcttc
     2881 ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaacccca atttccgata
     2941 aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct cacagcgatt
     3001 cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt tacgctgata
     3061 tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc ttgaaccgat
     3121 aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa gcggtgactt
     3181 caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga cttctcgttc
     3241 gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt aacgacgatc
     3301 gatttgttcg actgtttttt ctgtataaat cattttatct tgaaaaaagc ttctggcata
     3361 cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc aaccattttt
     3421 cttctgcttc tactcgtttt aaatagcgag gaagaaaagc ttgtttctct gcaactggtt
     3481 caactaaagc acctaattga gccacagtta caccacttgg aatatcccat gctggaactg
     3541 tattaatcat cgcactaggc aatatttcct ttatttcaga gaagaatttg tgacaatcac
     3601 tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa tcagaagcag
     3661 aaatatgtac atcttcaagt acagtttcta atctattttc tttccagcgg tatgccccag
     3721 cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aatattatcc acaccgatgc
     3781 aattagccgc gatggttttc agactagata ctcctactag ctctttatct aatgtataag
     3841 caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg ggaccatcag
     3901 aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac aattggtcga
     3961 ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt tgtccaagaa
     4021 tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct attcctaatg
     4081 taatcatata tttgatcctt tctgctatga cattctcgcc ttccattgta gcatactttt
     4141 ttattctgag gatgaaaata ccaaaggcaa aactagtaca ataatacatg attatctgct
     4201 ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac tccattttcc
     4261 cacagctttt ccatctttta tttcgttgat tctcgaataa tcaactctgt ttccacaata
     4321 atctttcttg aaaccggtgc tttggaatta cttaattgaa gaatcgtctc cactgctagc
     4381 tctcccatcc attcggtata aacttttaca gtagaaagcg agggcgttac atatttcgct
     4441 acactgacat cattgaagcc catcacagaa atatcttctg gtaccactaa acctttttct
     4501 tgaatcgctt tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag cgcatcagga
     4561 acatgcggat tcgtttttaa atattgcttc actgcttgat atcctgactc gacagaaaaa
     4621 tctgcttcga taattggacc ttcgtcgtag cctttttctg ccaaaacttg ttgaaatgcc
     4681 ataattctgg gatcttttag ccgctgatgg tttttcttcg tatattctgt tccagcaagc
     4741 atagcgatat gttgcgcttt atgggagaca aagtgttcaa tgactttttg aacacttgac
     4801 ttaaagtcga caactagaga gtcgattcct tgagaagtgc cgtcagaatc taccaaaagc
     4861 aaggcatttt gggatccttt catttctaat aactgagctt cgtcgaattt tcctagtgcg
     4921 atcgttccat ctgccggaag atcactaatg gaatcccacg tttctttcaa taaagtaacg
     4981 tttgcttctt ctgccttttt ttcgattcct aggcgaatag ataaataata aagatcttca
     5041 agctcttccg tatcgtcgta ccactggatc aggcgaagcg tttgcttagt atttgctgca
     5101 tttttacgat gtttcgtata atttaagcgt tctgctgctt caaaaatttt ttgctttgtg
     5161 gctaagccaa cggaaagatc aggatcatag ttgagaactc ttgaaacagt agcaggagaa
     5221 actcctgcca actgcgcaat gtctttaatt gttgccattt gtctctcctc cttttttacg
     5281 tttataaaat cgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct cgcttgaata
     5341 ctccagcgtc ttccagtaca cgtgcaaaaa cctgacctac agatttttgt acgacatcgg
     5401 tgaccgtatc tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc cacggacgat
     5461 gatattcagc catctggttt tcttctccta ataagaaatg ctctacttct actagttctg
     5521 gtttcaaacg aggtggtaaa acagcaagcc ccattacttc gatcaaccca atgttctcct
     5581 ttttgatatg ttggacatct ttatgtggat gaaaaatccc atcaggatgt tcttctgaaa
     5641 cattgttgtc tcgaaggaca aggtccaact caaagtaatt gtcccttctt cgtgcaattg
     5701 gcgtaatcgt atggtgtggt gtaccatctg ctgaaaaagc tctgatagat actgtttcat
     5761 ccgaatatgc tttccatttt tccaatactg agttagctgc ttcaataagc aagtttttct
     5821 ctgttgattg caaacggatc acagacatcg gccatttcac gactcctgcg atgacttgcg
     5881 gataatcttc tagtttgata agtttttcta tcggtgcttt tgccatagga aattcatgac
     5941 gtcctgcctg ataatgatca tgagaaagaa tcgatcctcc aacaattggt aagtcagcat
     6001 tcgaaccaac aaagtaatga ggcaagactt cagtaatccg aagcagtcta gcaaatgttt
     6061 cctttgtgat tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt tcattgtaat
     6121 aagcataagg ggaatattgg aatccccagc tttcaccgtc tagattcatt cgaataatcc
     6181 ggtgattggt gcgggctgga tagttcaagc gacctctata tccttcgttt tccatgcaaa
     6241 gcatgcaggt aggatagtct acttttaccg cctcgcgttc tgctgcgatt tgttttggat
     6301 ctttttctgg cttagataaa ttgattgtga tttccagttc accatactcc gactctgccg
     6361 gaaatttgat gttttttgcg atggctcttg ttttgatgta gtcatttttc ttacttaaat
     6421 taaagaaata ctcagttgct tcctctgggc ttttcgaata gtgctgtgca aagaatgcat
     6481 tgacgactga aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa atctcttgtt
     6541 ctgctggcaa ttctgaaaga acaccatttt ctcttgcctt agcgacaagc tgatccaata
     6601 agtctacaga ttcttcctta actggtctta cttcaatgct ttctagagca tcttctccta
     6661 ccatccctaa aagacgattc tgtaaatata agcggtccat ttccatccat ccgccagctt
     6721 cgatcgcaag tgtggcaaaa tcacaaatcg tttgactgat tgacatatct cattccccta
     6781 ttcttcataa ccatttggat ggctgacatg ccaatcccat gctgttttga taatcgcttt
     6841 gatatctgtg tattcaggtt tccagcccag aatctctctc gctttttcac ttgaagcgac
     6901 taatcggcta ggatctccag ctcttcttgg tgctatttct gctggaattt cttttcctgt
     6961 cacttctcta gctgcttcta gcatttcctt gactgagtat cctttgctgc tgcctaaatt
     7021 gaagaagttg ctttcatttc cttctttcaa gtattccaaa gctaaaatat gtgcagcaat
     7081 caaatcttct acttgaacat aatctctgat acatgttcca tctggtgtat cataatcgtc
     7141 tccataaaca gccaaggctt ttctttgacc aagagctact tgtaaaatga ttgggacaag
     7201 atgcgtttct ggtgtatgat cttctccgat agatgcatct gcttttgctc cggctacatt
     7261 gaagtagcgc aaagcgacat agcgcatgcc atatgcttgg tcacaccact tcatcatttt
     7321 ttccatcatc aatttacttt caccatacgg atttttagga ttagctggtg tatcttctgt
     7381 gattggagac tctttcggct ctccataagt tgctgcagta gaagagaata cgattttatt
     7441 cacgttgaac tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt tgttgttgaa
     7501 atacatcaat ggtttttcga cagattctcc tactaaagag cttgcagcaa aatggattac
     7561 tccttcaatc ggttcctttt caaatacgga acgtagaaat tctttatccc ggatgtctcc
     7621 ttcataaaaa tgagcatctg gatgtactgc ttgtttgtga cctgtgagca aattatctac
     7681 cacagctact tgatacccct tttgaactag ctggtctaca gcgtgcgaac cgatataacc
     7741 ggctccgcct agtactaaaa ttgtcataaa aaacactcct ttgattagta aaattgtgtt
     7801 tgttgattct atataattta aaaaacgaag tttactttta tggtgaaaac aaacaaagta
     7861 aaatttcgta aattcatatg gtaaatttat aataaaacat ttagtaaaca ttttcaaggg
     7921 ttttttgcaa aggtttacaa acaaacgtaa aactagcctt tttttctctt ttgttccata
     7981 atagatataa caaataataa atgaggtgga ttcaatgaaa aaatttgtat ctggaatatt
     8041 agtaggaagt ctggcaacag ccgctgccgt tgctggtttg gttgcttctg tgaaaaaaac
     8101 agtgatcgat ccaattgacg aaaaagaagc aatgatcgaa gaaaaccgta aaaaagcaat
     8161 gcgtaaacgc gtttctcgtt aataattatt taaaaatacg catgaagact ttctaaaaaa
     8221 cagataagtc atcatgcgta tttttatttg tcagtcaata taagttgtgc tattttatct
     8281 gcctgtctca gattccatgg atctgtataa ggtctagcta ctgctgcagt tagccaatcc
     8341 acaccttcaa caggtattcc atagcaataa aagtcagact ggatttcccc tgaagcatca
     8401 atcacgcgat tcgtacctcg ctcaacatct atagcttctg aatgataact tcctgtatta
     8461 tctgaaaatg aaaaagggcg aacaagtttg ttttccttca aattttttat gacaggatta
     8521 cttgaacgag aaaagcttgt ttgtggcaat cgagcttcaa tcaaatagcg actgctgatt
     8581 tctttatctt gtagagaaat aacatagcgg ttgtgctttt gctgaatatc aaatggcggt
     8641 tcgatcaaat gaaaaatacc cgcatctatc agtgctgcta attctttcgt ccgaatgatt
     8701 ggcggaccga tcgttaaaaa gctatttaga tgagtaaacc atccccatag tatttctttg
     8761 tattccttag cagaaaaaac gtgccattca atagcctgcc ggattggatc acgccagtct
     8821 tttaatgcat caaatgttga agcaaatgct cctgtgcaat tgcctttttc cgcttctttt
     8881 atctgccctt caataaatat tcggctagct tctgaaaagg gctcattctg ataggcaaga
     8941 ggagtttcta aatacgacca atcccatcta taagggatta gtgctggatg ctttccgaga
     9001 cactcctctg aagagaggga gagaaaatca tgctcaaaat ttttctttgg aatcggcaag
     9061 tgatattctt cgatgatttt cttgtaataa aacaactcaa cgtctttttt cagatagtca
     9121 aaaaatgttt cgccagtcaa aaatcccgac tgatgccatt gctccaggtt ttcttttgtt
     9181 agtaaccgtg gccatgccat tgctccgcct tgcttttgat ttcttcctcg agggtaataa
     9241 gggagccccc ttcttgatcc actgtataca atcggttcct tgcctgatgg atgatagacc
     9301 agttttccct tttcgttctc gaagcgccct cctcttgata ctgtaaacaa tccgacataa
     9361 tcaaaaaaac ttaagcctag acctcttaaa aagacatttt ctttaggcgg tatcttcgaa
     9421 agatctacat ctgcaggatt agaaggaagc tgatacagta aaacattttt ctttgcgtag
     9481 tctacccaca ctctttcttc ttctgtctca tcattttccc agtgaccgct tgctaaaaca
     9541 agctgatcga tagaatagct ttgctgatct gtctgtaaaa gataaccatc ttttcttttg
     9601 cttacttttt ttactaaaga ataaacaaac tgaatagaag ctggaaattc ttcttgcaac
     9661 tttgagaaaa accagcgttg atagagtccg tataaacatc gagtagattg ctgatctttt
     9721 tccagacgat tgatttcttc tataattagt tgcttatttt tggttaccgt ctgttcgata
     9781 taatctgtag caggtccttt actccattga aacaagttgg gtccctgtgt taccaatcct
     9841 ccactggaca atgtttcatc tgtaaaaagc gtcacttgtt gagaaacaga attcattaat
     9901 aactccggtg gctgattcaa ccgccaaact cgacctccag gaccattggg atcgaaaaga
     9961 aaaagttcaa gagaatgtct gtcctgatta ttatggagaa tcctttctgc tacacttaat
    10021 cctctaggtc cggcaccaat gatccctatt ttcatttgca tgcctccatt tcgataaatt
    10081 ttttcaataa aacagcatgg ttctgatgtt cattttttgc accataaaca aaagtaatga
    10141 ctggatgttc tttcataagt tgactgagtt taaaaatagc ttgcttcttt tcttcttcga
    10201 aaatcaattc ttgctggtat ttctttgcaa atgaagcaaa tcgttctggt acatggtcaa
    10261 aagttttgcg tgcttcatta ctaggtgcaa tttctttcaa ccacagatct aactgcgctt
    10321 tttcttttga aattcctcgt ggccagaatc tatccacgag tattctatag ccatcctctt
    10381 tttcagcatc aaggtaaaca cgttttaatt tgatcatagt tgtttccttc tttctacctt
    10441 ttcgcttagc ttatatatgt agataatagc acaaaataaa aaaaatgaac ggcacaacta
    10501 tccattgcgc cgttcatgtg atcttggtca aaatctaatg attgattcgg tttttaaagc
    10561 tgtcatacaa tcatctgttt tatacttttt tacttttgat ttttccagac catccgtcat
    10621 atccaccctt aagtaaataa atctcttgat aacctgcttt acgtaattta tttgctgtac
    10681 ggatgctcaa acttttcttt tgatcgtaaa tatagacagg ctggtctttt cgaatagatc
    10741 ctaatgaatc tttcaaaaca gtgtaaggga tgtttcttgc acctaaaata tgcccagcat
    10801 caaacacatc tttttcgcga acatcgatta cttgtgcttt tctcattcct tctcgaaatt
    10861 cttcttctgt caaagtcgtt gctgaacgtt tggccattac tcgtagatag acttcccaaa
    10921 ggatgattgc aagcacaatc aatagcaaaa tgatattaat tacccataaa accatgtaat
    10981 gcctccgttt ttttaagcaa atttcaaggc taatgaggca gcaccaatga caccagcttc
    11041 attaccaagc tctgctaatt tgatttttgt tgattctctt acttgcggga aagtaaattc
    11101 tttaaaatat ttctctacac ggctacgtaa aaattctcct gctgccgata ctcctccacc
    11161 taacacgata cttgacggat ttaatgtatt ccccaagttt ccgcaagcta aacctaagta
    11221 gaagcatacg cgatcaacaa ccatcaatgc aaacggatcg tcttcttgag ccatttcaaa
    11281 gatatcttta cttgttactt cttctccgtt gtctagcatt gctttcagtt ttgaatcccc
    11341 tgcgtattct tctgccaaga aacgacctag gcgtaccaca cctgtagcgc tagatacagt
    11401 ttctaaacat ccgcgtttac cgcaagtaca ttcgaaccct tcaggatcca cagtgatatg
    11461 tccgatttct cctccagctc cgctcacgcc gtgaatcaaa tttccgccag caacgatacc
    11521 tccgccaaca cctgttccta gagtaacgaa tacaacgtct ggatcgtttt ctccagcacc
    11581 tttccatcgt tcacctaaag cagcaacatt cgcgtcatta tcgatagcga atttgattcc
    11641 tgttcctgcc tcgatcagtt ttttcacaaa ttgaacttct gtccagttta agttatacgc
    11701 accaataacc gttccagctt cgctgtccac gcttcctggg gtacccatgc cgattccgat
    11761 aaagtcttcg gctttcatat tatataaatt caaacgatga ttgattgatt caatgatttc
    11821 aggaacgata tgcttaccat catccaaaat attggtatcg atgctccatt tttgctgaat
    11881 ctctccttct ggtgttaaaa ttgcaaattt tgctgttgtt ccacctaaat caatcccaat
    11941 aatcttcttg tccattacaa atttcctttc tttcggcttt cttcaattcg gtgctctctt
    12001 tttaatatgc gccacgctgt catgtatgta tcatgatcaa tcaaacggct atcatacaga
    12061 ttacgcaatt cgatagtcat taattcaatg tcatagatgc gtgcacccac atagataaag
    12121 atgccaaatt ttttgaacag ctgctgtaca tcatataagc tctccatctt taagcacttc
    12181 cttcatatta tacaagtaaa ccatattttt ttaaacccag taaaatacat attacaaaaa
    12241 gaaatacaaa aactagtgta gaaagaatcc tttggtgtat cgaatagctt gtctgttgtt
    12301 tcggtagacc caaaacattc cctaacacta atccgccaat tagcccaccg acatgtcccc
    12361 aaacatctac agaggtatcc agaagaccaa aaacaaatgt caacacaata aataaaagaa
    12421 aatgtctgac tagctgctgg atcgctgggt tatgtttaaa atgaaaccct aagataaaca
    12481 acgcaccgaa caaaccgaat atcgaagtac ttgcaccacc agacagaaca ttcgcttcgt
    12541 taaaagcaaa gcttgctgta tttcccatta tcccgctgaa catataaatc aagaaaaaac
    12601 gccaatggcc atagattgcc tcgatttgct gccccataaa atacagtatc agtgagttca
    12661 ttacgaaatg catcagtcca aaatggatga agattggtgt cactaaacgc caccattcat
    12721 tgaagtgaac aagataagga ccatacattc cggcataata agggatctca agtcgcggaa
    12781 ataattccat catgaggaag accgctgtct gtatccctaa aaaagcatag gtccataacg
    12841 gtcgtcttaa ccatgcacgc aattttattt gctgttgata attcatgaaa acctcccatt
    12901 tcattttagt caataaacaa ttgttggatc ttctgatcaa agctctccgg ttgccattgt
    12961 tcattcaatt gttcacgaaa aacaaggcta catgtttttt tatgaaaatt atttaaatac
    13021 cggtcgtaat aaccaccgcc aaaaccgatt cgataaccgg ctctattgta gataatacct
    13081 ggtacaatga ggagatcgat catctccggc aaatagagct tctctgattg tggctcaaga
    13141 acaccaaaac tcgaacgtac aagaacggac tgttcatcca tttcataaaa cgccatctga
    13201 cgtttcggca aagttttagg tacaactatt ttcttttgtt ctttttctgc tcgttggata
    13261 accggctgtg tatccagttc aaattcattc gataaagtcg tccctatcac ttttgctgac
    13321 ttccagtact cgctggcgaa tagctgttta tagattcgct gttctttttc ctttttcttg
    13381 ccacttttgg ccagattttt cagcttttcg attcctttcc ggcgtaatct tactttcaat
    13441 tggtccaaaa actcaccctt ccttctctca taaaatggta taggaaaaaa agaaaaatag
    13501 caatcgaact ttcaaataaa tgcataaata gaataaaaag aagacctaga aatgattggc
    13561 cttccttttt taatgaattt caactgttca gcgcgtgttt ttgtctgata aaaagtgcat
    13621 acagaaaata tggcgcacca aaaagggcaa tcaaaattcc tgtaggtact tccccacttg
    13681 gtagaatcac tctagcaacc atatctgcac taagcagaaa aatggaaccg aacaaaccgc
    13741 tcagaacaaa ggtccagtta ttttctttac gtacgactag cttcgcagca tgaggagcta
    13801 gtagtcctac aaaagataaa ctacctgtca ccgctacact accgcttgcc aaagcaacag
    13861 ctaacaacaa aaggattttt tgacttttag ccacgttaag gcctaatcct tgcgcgcgtt
    13921 cttgaccaaa tgatagcaat acaatcctct gtgcataaag tcctcctagc ggaattaaga
    13981 taaccgccca tggaagcaat gcccaaacat aaggccagct cgcaccccag attgatcccg
    14041 ctaaccatgc attcacaaat ccataatttt ccttagataa ttggatcgtg catagcaatg
    14101 ttaatgcaga aaggccggca ttaacggcaa taccggacaa taagattcga ttcataccca
    14161 aaaagccatt tcgatcatag gccgcaagat agacacagca agcagctaaa acaccaccta
    14221 gaaaggctac aactagcaat agaaacggca gcgaatagtg cgaaaagaaa cctaaataaa
    14281 gcatcactaa aaatcctgct ccggcattaa tgcccaatat actagaatct gccaattcat
    14341 ttcttgttac tccttgcaag atataaccag aaacgccaag acaagctcct gctaataaac
    14401 tgatcagtaa tcgaggaact cgaaattctc taacaatcag cgccaaagaa gatgattctt
    14461 tccctatgaa cgtggcaaaa agctgatcca ggtaaataaa aggcgtacca atcattaaac
    14521 tacctataga caggatgaca agtacaaaaa gcaagaaata gaacgtccaa tatatttttt
    14581 tcaccgttgt tccctcctta cttgtatcag taaaaacgga acgccaatca atgcaatgac
    14641 taatccaaaa ggtgtttcaa aagggggatt gatcatcctc gctgtaaaat ccgccaacat
    14701 aaccaatcca ccgccaacta aaaaagagac aggtagaaca taacgatagt ccgttccaac
    14761 cattccgcga acaactggcg gaaccaacag cccaataaat gaaacaggtc cgactaacga
    14821 aacggcaatg gctgctaaca ctgcaactag acagaaaaca atcaaccgcg ttcgtttagg
    14881 atgacttcca agagctaatg catgtgtttc actcaaatga atcaaagata ttgcttttcc
    14941 taatgaaaaa gaaaccaaaa cagcaagtat atataaagga aacaatactg ctaattgttt
    15001 ccatgtgata ttggcagctc cgcctacaaa ccaaaaagca agatcctgct gcagattaaa
    15061 gaattgggta agtgcttggc tgatagcagt aaaaaaagaa ctaatagcta cgccagctaa
    15121 aatcagctgt aaaggattca tgcccatttt cgaaaactgc aaaaaaccaa atacgagcaa
    15181 agttacagca aaagaacctg ccaatgacat tagaaacaca gaaaaatcag aagctttagg
    15241 ccaaaaagca aaactaaggg ccattgccaa cgaagcaccc gcgttgattc ctagtaagcc
    15301 tgaatctgct aaaggatttc ttgtcacccc ttgcatcaat gcaccgctca atgcaaaaca
    15361 actccctaca agaaatgctc ccgccacacg aggcaaacga atatttcgta tgatttggtg
    15421 actttgtttt gatctatcaa aatcaaacaa cgcctgccaa aaatctgctt tttccacagt
    15481 cgctgcgcca tttgaaagag caacaaaaaa aataatcaaa agaacaaaac agaccaaaag
    15541 caggagtcta tttttcttca tagtcctcat aattgatcct cctgctcttt tggcaacaaa
    15601 tcataggtta gaaaaactgg tttatttgtt cttggaagcg tgacaatcgt tgcatcaatc
    15661 tcaaatattt ttttcataca ctcttttgtg atcacttctt caggtgtacc ttctgtgatc
    15721 aacttaccgt ttttcattgc taataaataa tctgaaaaac gtgaagcatg attaatgtca
    15781 tgaatcgaca tcaagatcgt tgtttgtttc atttgattga tttcagtcaa taactgtagg
    15841 atctctaatt ggtgtgccgg atctaaaaat gttgtcggtt catccaagat caagatatct
    15901 gtttcctgag caagcgccat agcgatccag acacgctggg tttgtccacc agacagcgtt
    15961 gcgatatccc gcatacgaaa tccagctaga cctgtagcat ccaacgccca ctcaattgct
    16021 tcatgatctt gcgtgtccat gccactgaac agtttttgat aagggtatcg tccgtaagat
    16081 actacttctt ctactgtcat acctgtaggg tgttcactag tttgtgctaa taatgcaatt
    16141 ttttttgcaa gttcttttga actgtatgta gagatatcag agttattgac aatgatcttt
    16201 cctgatttca caggtagtat acgggtaaaa cttttcaata aagtagattt tccacttcca
    16261 tttggcccaa tcaaactagt gattttccct tcaggaatcg taacagacat gttttcaatg
    16321 atttttttgt tttcgtagcc ggctgcaatg tcagctgctc ttaatctcgt catttcaccc
    16381 atcctttcgc tgaaaatcat tctcaattat gacgctgttt caacatttcg tcaagcgcta
    16441 ctttcatatc tatagccaaa tgaagcggtt gacagcgttc gaaatatcag gaataataac
    16501 caatgagaaa gattctcaat tatgttagga ggaaagaaat gaaattaaaa acaaaagtat
    16561 tcacattatc tagcatattg ctgctggctg gagtactgag cgcttgctcc ccctcttcag
    16621 ataaagaaac agctacaact gccaattcga cgacttcatc caatagtact catacagtca
    16681 cagatacact tggacataaa gtagagattc caaacgcgcc caaacggatc atcggcagtt
    16741 acttagaaga ctatctgatt gcactagggg aaaaacctat cgcccagtgg actgttggca
    16801 gtggttccat tcaagattat ctgcaagatg atttaaagga tgtcccaacg atttcttatg
    16861 accttcctta tgagaaagtg ttaagctttg agcctgattt gctgttgatt tcttcttccg
    16921 ctactgtcga aggcgggaaa tacgaacaat atagcaaaat cgcgccaact tatgttgtaa
    16981 aaaacggaac agatgtcaca tgggaagaac aactaaaaga tgtcggcaaa gctttggcaa
    17041 aagaaaaaga ggcagaaaaa atcatttccg attatcaaaa atccatcaag aaaactcgag
    17101 aagaactagc agataaaatc gagaacaaga cagctgcagt gttgtgggtg accaacaact
    17161 cagcctttat ggtcagcaaa aatcggtcaa gtggaagaat cgtatacgac gatctggaat
    17221 ttggtgttcc tgatctagtg gaagaggtct ccaaaaaagc cacttctgac tggtctgctg
    17281 tttcctctga aaagctagct gaattagatg ctgactacat catccttgtg aacagtgaca
    17341 aagatgctgc catgttcaat gaaccaagct ggaagaatct aaaagcagtg acagacaatc
    17401 atgtactgca attcggacct gaatctagtt ggctatataa cggacctatt gccagtcagg
    17461 aaatggtcaa tgatatcaaa gaaaaactaa attgatttta actaaaaaaa gcatcttcac
    17521 gattagtcgt gagatgcttt ttttcttatt aacaaactag acgcatagat tttattcttc
    17581 ttttgaagca accctatttt ttactctttc ccatttttga aaacgatggg catatagatt
    17641 ttcttgatcc gttatcccct catactcctc tataagggcg aagtctgaaa aatcaatatt
    17701 cccaatatac gtatcgcctt caaacgtgct atcgatcaat gttcgccaga tccgaccagt
    17761 gtaaggaaga agttcttgaa aaatccgact accacccgaa acatagatat cttctgagat
    17821 cgtttgacta tacgacaaaa gttcatcgat cgaatgcatt acttccgcat tttctttcgt
    17881 gcgaaaattt ggttgcgttg tcaacacgat gatatggcga taaggaagtg aaagatcccc
    17941 cattccttca taggtctttc ttcccatcac taaaattcga tccattgtat gttcgcgaaa
    18001 gaaacgcata tcatttggca atcgccatgg taacaaacca tttttgccga tcagaccatt
    18061 tttatcttgt gcccacatgg aaataaacat attatccgct ccttatgatt ttagatgttt
    18121 tgagataaaa aaagtctcaa ttctttgacc agcaaggaat tgagaacttt aaaaacatta
    18181 ttttgtttca cggtgcaaag taactttgcg ttcgcgtggg caatattttt gtttttccaa
    18241 acgatcagga ttgttacgtt tgtttttgct tgttagatag ttacgttctt tacaagaagt
    18301 acactctaaa gtgatgttta cgcgcatgtt tttccctccc atttttacaa gaatcctcta
    18361 agatgactct cagacttgat tatcttacca tgattttttc tagattgcta gtgtttttca
    18421 cgattttgtt aagtaaaact acttgacgat aaatacaacg gttttacttc tcaaacaaaa
    18481 tctttttctt tttgtataaa tacccttaca cttgtttata tagattatgt tttttaataa
    18541 agggggatac taatatgcca tggaacatga aggactttcc tgcatcaatg aaaaacttag
    18601 ataaattgac aagaaaaaaa gcaatcgaca tcgcaaatgc attattagat gaagggtacc
    18661 ctgataaccg tgcgatccca attgccattg atcaagcaaa aaaatggtat gaagatgcta
    18721 gtgaatcaga aaaacgaact ttcgaaaaag aaaaaaatcc aagcaagacg gatgaacacg
    18781 atacgaatcc tcgtgctgga aaactgcttg attcagatgt gatcgtagaa tatgaggaag
    18841 aacaatggat cgtgaaatct aaaggggcaa aaaaggcaag caaccatttt gatacaaaaa
    18901 aagaagcaat cgaaaaagga aagcaagtag cacaaaacaa agaatcaact ttgttcattt
    18961 ataaaaaaga tgggacaaaa gaaaaagaaa tcgattattg aacatatgta aaggggttgt
    19021 gacgaaaatc gtgtcacaac ccccttatgt tcgaaacaat ggtgttcctc tgccaactcc
    19081 gaaccataag tcataatatc tataaaacgg gaagagctgt tttttgaaat tgtttcttat
    19141 cactcagtgt taaacggtag gagaaaacct cttttttatt gatatttgat tttcatcaaa
    19201 cagtcacata gtcgattgct tctgcttgcg gaatcgtttc atctacaaag tagactttat
    19261 accatgtcaa gaaactgaag attgtccaga tttttctttg ctcttcttta cgtccttcgt
    19321 gatggtcatc tagcaacttc atgatttttt cttgatcaaa aaattctttt gcaaaatctg
    19381 cttcgaataa tgctttgact tgttcgtagc cgtgttcttc tttcaaccaa gctttgattg
    19441 gcacagggaa tcctaatttc acacgattcg accattcttc cggaagatgt ttgttcgcag
    19501 cttgtctgaa aatatctttt gtgttgtttt gattcaataa aaacttactt ggtatttgtt
    19561 gagccaattt catgacttcg atatccaata aaggtactcg gacttccaaa gaacttgcca
    19621 tagaaagttt atctgccttt aacaagatat cttttggcat ccattgatga agatcgacat
    19681 attgcatctt attgacttca tctttgattc cttctgtttt ctcgtaatga acagtcatga
    19741 tttcatccac agatggtgct gtttgatatt ttggtgttaa cacttctgat gcttctgatt
    19801 cttcgaacac tcgagcatga ccgataaaat attcttttgc aggagcaagt gattcatata
    19861 aatggatccg tccatggaag tttttcattt ttccgatttt acgtcctaaa ttatagcgtg
    19921 ttccttttgg taattttttc aatccttgag caatcacacg gatgaatttt gaattcgtat
    19981 ggaatccata cgtttggtat cccgcaaaca gttcatccgc cccttctcca gattggacta
    20041 cccgcacact ttgtgaagca agatttgcta aaaagtacaa aggtacgcaa gaaggatttg
    20101 aatcaggttc atctaagtga tactggatca aagggaatgc tttgaacgac atgtctccgt
    20161 cgatcactgc tgctgtatta tctaagtcca atagttcggt tagttttctg gcttcaatcg
    20221 cttcattata ggttttatca tcaaacccga ttgagaaaga atgatctggt ttcaatacag
    20281 aggttacata actggaatca accccgctgg ataagaaaga accaacttct acatcactga
    20341 ccgtatgtgc ttcaatagaa gcttgaacgg tttcgtcgat tttttcgatc cattcttgtc
    20401 gggaatgcgt ttcttttgtt tcaaaatctg catcccaata ttgctgaatg tctatttttc
    20461 catctttata cgtataataa tggccttcag gcaaacgata gacatctttg aagaatgttt
    20521 cgccgtttaa tggagaatat tggaaagtca tataaggttt caacgcttct ttgttcaatt
    20581 ctttgttaaa atcaggatga ggcagaaaac ttttgatttc tgaaccaaac ataaatgttc
    20641 cgttcatttc tgcataataa tatggtttga tcccaaagtg gtctcttgca ccgaataact
    20701 cattcttttc attatcccaa atagcaaaag caaacattcc gcggatcttt tgcaataatt
    20761 ctgttcccca ttcttcgtaa ccatgcaaaa ggacttctgt atctgcatgt gtttgaaaaa
    20821 cgtggccagc tgcaatcaat tcttctctca aaggctggta attatagatt tcgccattaa
    20881 aaataatgat ctttgtccga tcttcattgt aaataggctg agtaccgccc tctaagtcaa
    20941 taatacttaa tcttctgaat cctaaagcaa catgtttatc gatatattct cctgaactat
    21001 taggtccacg gtgaacgatt cgatccatca ttgtattaat aattgttttt ttgttgtcct
    21061 tgtcgttaac aaaacctaca ataccgcaca tttaactttc acatcctaat ataaaattac
    21121 tgtttatttc attcatatgc atcttatcat agaataaata gcggacgaat attttctctc
    21181 ttacaatttc gtcacgtgaa aacaaaagat gaaagtttct gtttttacag aaaataaagt
    21241 cccaatatct aatttaccat gttttctttt ttgaggctac cttattctct tcgtgttaaa
    21301 aaaatattag tggatgctat ttccaaaaaa accagcaaaa aaagacacga cctaaagatc
    21361 gtgt
//
LOCUS       DAZKBH010000040        20485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000040 DAZKBH010000000
VERSION     DAZKBH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.040"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ACG8OO_001414"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ACG8OO_001414"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFP9045302.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ACG8OO_001415"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ACG8OO_001415"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFP9045303.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ACG8OO_001416"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ACG8OO_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFP9045304.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ACG8OO_001417"
     CDS             2857..3732
                     /locus_tag="ACG8OO_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP9045305.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACG8OO_001418"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACG8OO_001418"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9045306.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ACG8OO_001419"
     CDS             4629..5063
                     /locus_tag="ACG8OO_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9045307.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ACG8OO_001420"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ACG8OO_001420"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9045308.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ACG8OO_001421"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ACG8OO_001421"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9045309.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ACG8OO_001422"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ACG8OO_001422"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9045310.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ACG8OO_001423"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ACG8OO_001423"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFP9045311.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ACG8OO_001424"
     CDS             13334..14260
                     /locus_tag="ACG8OO_001424"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFP9045312.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ACG8OO_001425"
     CDS             14442..15335
                     /locus_tag="ACG8OO_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFP9045313.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ACG8OO_001426"
     CDS             15469..15594
                     /locus_tag="ACG8OO_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFP9045314.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ACG8OO_001427"
     CDS             complement(15728..16360)
                     /locus_tag="ACG8OO_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045315.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="ACG8OO_001428"
     CDS             complement(16508..17050)
                     /locus_tag="ACG8OO_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFP9045316.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="ACG8OO_001429"
     CDS             17185..17574
                     /locus_tag="ACG8OO_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045317.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="ACG8OO_001430"
     CDS             17578..18342
                     /locus_tag="ACG8OO_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9045318.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="ACG8OO_001431"
     CDS             18471..19418
                     /locus_tag="ACG8OO_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9045319.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="ACG8OO_001432"
     CDS             19548..20288
                     /locus_tag="ACG8OO_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045320.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5495 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtt
//
LOCUS       DAZKBH010000041        20470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000041 DAZKBH010000000
VERSION     DAZKBH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.041"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            378..947
                     /locus_tag="ACG8OO_001433"
     CDS             378..947
                     /locus_tag="ACG8OO_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFP9045321.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            934..1794
                     /locus_tag="ACG8OO_001434"
     CDS             934..1794
                     /locus_tag="ACG8OO_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9045322.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            1984..2688
                     /gene="yycF"
                     /locus_tag="ACG8OO_001435"
     CDS             1984..2688
                     /gene="yycF"
                     /locus_tag="ACG8OO_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFP9045323.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2693..4528
                     /gene="walK"
                     /locus_tag="ACG8OO_001436"
     CDS             2693..4528
                     /gene="walK"
                     /locus_tag="ACG8OO_001436"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFP9045324.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4525..5841
                     /gene="yycH"
                     /locus_tag="ACG8OO_001437"
     CDS             4525..5841
                     /gene="yycH"
                     /locus_tag="ACG8OO_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFP9045325.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5842..6711
                     /locus_tag="ACG8OO_001438"
     CDS             5842..6711
                     /locus_tag="ACG8OO_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFP9045326.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6766..7575
                     /locus_tag="ACG8OO_001439"
     CDS             6766..7575
                     /locus_tag="ACG8OO_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9045327.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7678..8307)
                     /locus_tag="ACG8OO_001440"
     CDS             complement(7678..8307)
                     /locus_tag="ACG8OO_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9045328.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8477..9769
                     /locus_tag="ACG8OO_001441"
     CDS             8477..9769
                     /locus_tag="ACG8OO_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9045329.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9845..10507)
                     /locus_tag="ACG8OO_001442"
     CDS             complement(9845..10507)
                     /locus_tag="ACG8OO_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP9045330.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10732..11016
                     /gene="groES"
                     /locus_tag="ACG8OO_001443"
     CDS             10732..11016
                     /gene="groES"
                     /locus_tag="ACG8OO_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9045331.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11067..12692
                     /gene="groL"
                     /locus_tag="ACG8OO_001444"
     CDS             11067..12692
                     /gene="groL"
                     /locus_tag="ACG8OO_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9045332.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(12781..13920)
                     /locus_tag="ACG8OO_001445"
     CDS             complement(12781..13920)
                     /locus_tag="ACG8OO_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9045333.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14031..14615)
                     /locus_tag="ACG8OO_001446"
     CDS             complement(14031..14615)
                     /locus_tag="ACG8OO_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFP9045334.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(14742..15173)
                     /locus_tag="ACG8OO_001447"
     CDS             complement(14742..15173)
                     /locus_tag="ACG8OO_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9045335.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(15191..15511)
                     /locus_tag="ACG8OO_001448"
     CDS             complement(15191..15511)
                     /locus_tag="ACG8OO_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9045336.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            15811..15951
                     /locus_tag="ACG8OO_001449"
     CDS             15811..15951
                     /locus_tag="ACG8OO_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045337.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            16249..16506
                     /locus_tag="ACG8OO_001450"
     CDS             16249..16506
                     /locus_tag="ACG8OO_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFP9045338.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(16477..16785)
                     /locus_tag="ACG8OO_001451"
     CDS             complement(16477..16785)
                     /locus_tag="ACG8OO_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045339.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            16863..17609
                     /locus_tag="ACG8OO_001452"
     CDS             16863..17609
                     /locus_tag="ACG8OO_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP9045340.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            17624..17827
                     /locus_tag="ACG8OO_001453"
     CDS             17624..17827
                     /locus_tag="ACG8OO_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045341.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            17932..18231
                     /locus_tag="ACG8OO_001454"
     CDS             17932..18231
                     /locus_tag="ACG8OO_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045342.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            18231..18413
                     /locus_tag="ACG8OO_001455"
     CDS             18231..18413
                     /locus_tag="ACG8OO_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045343.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(18419..18895)
                     /locus_tag="ACG8OO_001456"
     CDS             complement(18419..18895)
                     /locus_tag="ACG8OO_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045344.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            18948..19673
                     /locus_tag="ACG8OO_001457"
     CDS             18948..19673
                     /locus_tag="ACG8OO_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFP9045345.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            19847..20188
                     /locus_tag="ACG8OO_001458"
     CDS             19847..20188
                     /locus_tag="ACG8OO_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045346.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            20181..>20470
                     /locus_tag="ACG8OO_001459"
     CDS             20181..>20470
                     /locus_tag="ACG8OO_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFP9045347.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         6892 a         3454 c         4134 g         5990 t
ORIGIN      
        1 caaaaagtac ttaataatag tagcactaag aattttaaaa tgcaagacta catttaaaga
       61 aaatcagaaa aatctttact tccttatagg aaatcattca tagattattc acatttgctc
      121 gctattatat aagcatacca acaaacaaag ttcttgcaag agaacaaacc ctaacacttt
      181 tttcattttt tactcctttc ccgccgtctc cccggcgggt ctcctgttct taacgataaa
      241 agacaagaag gcttagcctt cttgtttttt gttttcatgg aatcccgcta atcaaatcac
      301 ctagttaaag caactagatt gaatgtttat cataagtctg atatcatgtt tatgaaatga
      361 aaaaaggagc ggttatgatg gaaaaaatgc gtcagcaatt aatggagtgg gggaaggaac
      421 tagaaacatt ccgtctgcct cactgggaag aactccctga cttagaactt tatatggacc
      481 aagtcagaac gttagttgat cgttatcttt cacctgtgat acaaggagaa aagcatccat
      541 tgcttacctc ttctatggta aacaattacg ttaaactagg tttgatacca gcaccagtta
      601 aaaaaagata taacaaagaa cacgtagcat ttttacttgc aatcactacg ttgaaacaag
      661 tattgactat tcctgaaatc aaagaaggga tattgttcca aggaaaaaca gtaggaattc
      721 gtgaagccta taatctattt tgcgatgaac aagaagcagc tgtgtggatg gtcagccaat
      781 tagcacaagg taaatctcat ccgcaaaaat tcgaccaggc aacccctgta gaatatattg
      841 ctgtaagagc ggcaacttta tcgtttgcaa tgaaacttct tgcggaaaaa acgattgttc
      901 tagaaaccga atatttaaaa gaggagaaaa caaatgaaaa acaataaaat cgcaatttta
      961 gtagattcag gtacggatgt tccagaagaa ttgataaaaa aatacgagat gtatatgatc
     1021 ccattgaaaa ttatttataa agatcaggta tatacagata aaattgacat tacccctgaa
     1081 gagatctatc aacgtttgcc agaggaaatc cctggtacat ctttgccgga tggtgagtcg
     1141 atcacagaaa tctttgagca aatcaaaaaa gacggttatg aaaaggtact tgcggtaaca
     1201 atttccagtg gattgagcgg gacatataat gtggttcgtt taattgcaca acagcaagaa
     1261 gaattggaag catatgtact agatacgaaa aatattggta ttggtgcagg tttttctgcg
     1321 atccaagcag caaaatggct ggaagaagga gtagaatggg atcaactgat cagtaatctg
     1381 aatgagttag tcgaaagaac aaaagttttc tttaatgttg cgacattgga atatcttcaa
     1441 aaaggtggac ggatcggttt agttgcttct attgtaggaa ccgctttaaa gcttaaccca
     1501 atcatctcat gtaatgaaga agggatttat tacacagtag gaaaagctag gggacgtaaa
     1561 aaaagtttgg ataaaacggt tgcttacgtc aaagaaaaaa tcggtaatgc aaaggtcttc
     1621 aacttagctg ttgcccatgg ggatgcgaaa gaagaagcaa ttgaaatgga agcgagactt
     1681 aaaagagagt tcccacaagc aaaccaaatc tactttggcc agatttctcc ggcattagta
     1741 gtccacactg gtccgggatt attgggtgtc ggtgtacaag ttttagaagg ataaatcaaa
     1801 aaaaggaaac actgaggtga aaatcagtgt tttccttttt tattgaagtt agaacaaaaa
     1861 ataataaagt attttgcact ttcattttaa ttttctcttt tgttttatag gcaaagatga
     1921 aacaaaagca gaaatacggt ataatgagaa ggtatgacaa taatatataa gaggtggctc
     1981 tttatgaaaa aagttttagt tgtcgatgat gagaagccga tttcggatat cgtcaaattc
     2041 aatttagcca aagaaggcta tgacgtttat actgcatatg atggggaaga agccttggaa
     2101 aaagttgctg aggtagaacc tgatttgatt ttacttgatt tgatgctacc taaaatggac
     2161 ggcttagaag ttgctcgtga ggtacgcaaa acttatgata tgccgatcat tatggtaaca
     2221 gccaaagatt ctgaaattga taaagtccta ggcttagagt taggcgccga tgattacgtg
     2281 accaagccat tttccaaccg agaattagtt gcacgtgtca aagcaaatct tcgtcgcggt
     2341 gccacggctg cgaaggaacc agaagaggca gcacctgcgg aattgacgat tggtgatttg
     2401 acgatccatc cagaagctta catggtgacg aaacgaggag aaacaatcga gttaacacat
     2461 cgtgaattcg aactactctt ttacttggcg aagcaccttg gacaagtgat gacaagagaa
     2521 catttattac aaacggtttg gggttatgat tattttggag atgtacgaac agtggatgta
     2581 actgtacgtc gtttgcgtga aaagatcgaa gataatccaa gccatccaaa ttatttagtg
     2641 acacgtcgtg gtgtcggtta ctatttgcgt aacccagaac aggagtaatt ggatgaaaag
     2701 aaaagtccgc ttttttcgat cagtcaattt taagatcgct attaccttta ttctgatttt
     2761 attgatctcg atcgaaatta ttggtgccta ctttatacgt ggtttagaac gttccacgat
     2821 caatacattt attaaagata tgaatcaaac ggtggaatca ttagcaacaa caatcagccc
     2881 ggaattgaat cgaaaagata atgcagatga tgaagaggta aatgcgaata tcaaaagatt
     2941 tattgaaaac agtgcgacta gcgatatcat cgaaatacgg gtcgttgacg aaaaagggat
     3001 catccgagga acaaccgatg tcaatgaaca aagcgctgtg ggaaagaaaa atgattatgt
     3061 tgatatcaat gatttcacga ctaaacgtta tgttgcttta gataacgacg ataaacgggt
     3121 gaatatcaat gttcaaccga tcctgtcccc gaccggcgat gccgttatcg gggctcttta
     3181 cgtcaaaagt aatatcgaac aaaaatattc ggaaatcaat aacacagcag tcattttctt
     3241 tactgcctca ctaattgcgg catttatttc aatgatcgta tctgttttag ttgctcgttc
     3301 gattacacag ccgatcggtg aaatgagaga acaagccatt cgaatcgcta gaggagacta
     3361 cagtcggaaa gtcaaagtgc atggacaaga tgaacttgga cagctggctg ataccttcaa
     3421 tcagttagct gaacgtattg aagaagctca agatacaatg gaagctgaac gcaatcgttt
     3481 ggatagcgtg ctatcccata tgacagacgg cgtcattgcg acagatcgca ggggtaaagt
     3541 cattacgatc aatgatatgg ctgtttcctt gctagatatc aaaaacgagg aagcaatcgg
     3601 acaatcgatt ttgacacttt tagatatcga agaagaatat acgttgcgga aattattaga
     3661 agatccaaac gaaatggtac ttgaccgttc aaccagtaca ttggaaagtg atcagatgat
     3721 cttgcgagta gacttttcta tgatccgtcg cgaatcaggt tttatcagcg gattagtagc
     3781 tgtcttgcat gatgtgacag aacaagaaaa aacagaacgg gaacgtcgtg aattcgtctc
     3841 taatgtttcc catgaacttc gtacaccgtt gacaagtatg cgtagttata tcgaagcatt
     3901 gagcgaaggt gcgtggaaag acgaggaagt agcaccaaat tttttgaagg taacgctaga
     3961 tgaaacagac cgaatgattc gaatgatcaa tgacctctta aatctatctc gcatggatac
     4021 aggtaatacc cagcttcaat tagaatatgt caatttcaac gagttagtga attttgttct
     4081 tgaccggttt gatatgatgg tgggaaatca ggaaaaaaat tataagatcc gtcgtgaatt
     4141 tacgcaaaga gatctttggg tcgaagtaga tacagataaa atcatccaag tagtcgataa
     4201 tatcatgaat aacgcgatca agtattcacc agatggggga acgattactt gtcgcttatt
     4261 agaaacgcat aataacgtga ttttaagtat caccgatcaa ggattgggga ttcctaaaaa
     4321 agatctgaac cgagtgttcg agcggttcta tcgagtagat aaagcacgtg caagagcaca
     4381 aggtgggaca ggacttggat tagctatttc tagagaagtg attaaggcgc atcgaggcgc
     4441 tatttgggca gagagcaagg aaggaaaagg gtcgactttt tatatctcgc tgccatatga
     4501 accttatgag gaggaatggt gggaatgaga atctctgaaa aagtcgttcg tataggactc
     4561 attttgatgg ttgcgttaag catatacttc tcttatgcaa tctggctgag cccagcagga
     4621 aaaagttcaa tcaacgtaga tgagacaaac actcaaatga tcgagtctca aagcaatcga
     4681 aaaacttccg aagtctttct tccgcttcat gttacatggc accattcggg aatgatccaa
     4741 gaaaccaaca gtgaaaatct cgtgtaccga ttgcaattga tcatcgataa agcaagattt
     4801 ggaaagcttt ctgaagtagt ttcaggagac aaatcacagt ttgaacaggt gaaaaatctg
     4861 aactctggtt ttgagttggc ctataatgcg ccttttttgc taagagaata caaggaagca
     4921 tttgatttag atttagatgt gtcgaacttg gcaaaagaaa gcgaaaatat gtattttact
     4981 agaatacaat tcgatcaagg aaataaaaaa atccgatttt tgaattattc gaaccatctc
     5041 gtatatgaag caaatatttc gttgaattgg acagatatca ataaagaatt aacagcaaaa
     5101 gacacaaaat ggacagaaat gacaacagaa ccatcgacga tcgacacgca atatgctacg
     5161 aaacattcga tcaaactgaa gaagtatagt tatatcctgt cacagcaacc ctatacactt
     5221 tttagaaatg cttttttcca gaaaccagat agcgtgaaaa ataatgatga aagcaatgaa
     5281 ttgatttttt acggtgggaa tgaaacaatg tctatccatt cggaaacgca agtagttgat
     5341 tttcgtggag agctgccaga gaaaaaagaa aatgctagtg tattttctga aagtttcccc
     5401 tatattagca aattaggcag ttctttaggg aacctcagat attttgaccg cagtgacaat
     5461 acgatcgatt atcggatatt cgtggaaggc ttcccggttt ttgggacaga cagcagagga
     5521 cagattggtg tggaagttgg cggtgaaacg aatggttctg tgcaagtgaa tatccaaacc
     5581 agtttgaata cgattcaagt gccgatacct tcagacgaag aagtagaact tgcaagtacg
     5641 aatgaaatca tccagcagtt aaaagctaag ggagcagata gcaacaagat tggttcagtg
     5701 atcgttggct atacatggca aaatgtcaaa gaaacgaatc gtgtcgttga tctcttacct
     5761 gaatggtatg tgaaatataa agataactgg tattcagcta acgaccttct ggaaaaatta
     5821 cctggattgg aggcgaatta attggatttc aaaaaaatcg aatggatatt tttcgtcgct
     5881 tttttagggt tgaatgtgtt tttattcagc agttatcatg acgcggtata ccaagaaaac
     5941 aatgtcattc gctctaatca gactgaatca atccaacaac gtctggctag cgacgatatc
     6001 acttacacgg ggaagatttc gtctgagaac aagcaaggct attatcttag cgcggagcag
     6061 accaatctta gcacttcatt agcgaattat cgcaaggctg gtaaaagcgg cttgctggca
     6121 agtggtgtgt ttttagacgg tgatgttttg acgaaatctt tatccagttc agatgctgaa
     6181 aagaacgtga tcgatccaga taaaattagt agttccttgg aagatttttt atctaacaaa
     6241 gacaatattt tattcggtaa tgattatgtg tacacgaaaa acttttctca tttagaagaa
     6301 gaaccactag aaatccaagc aagccaagag tatgaaggca tcccattcaa tgatgataca
     6361 gcaaaaatcg ttctttcttt agaaaaaaac gataatgatt atagcatcac gaaatacatg
     6421 cagacgcatt tatctgatat cgagcagctg cgcgaaaaga cggaactgta tacggaagag
     6481 gaagcaatca atacattgta catcaataac aaaataccaa gaggatcaaa aattttatgg
     6541 cgtcagctag cttattcctg tattctgaaa gtccgggaaa aaaatgtgta tgttcctgtt
     6601 tggcatgtag cgatcgaaac aagcgataaa gtggtccaaa ttgagagcgt caacgctttc
     6661 agcaatacga tcgtgacaaa caatactgta ccaaaagtgg aagatcaata aaaaagcgag
     6721 tataatgaaa aaagtttaca aagtaagaag ggaagtagga gtcaaatggc tgaagaaaaa
     6781 ccagctttta aaatcagtat cctagcaagt ggaagttcgg gtaattcact ttatatcgaa
     6841 agtgaaaaaa aacggctttt ggtcgatgca ggacttagcg gtaaaaaaat cacttccttg
     6901 atggctcaaa ttgatcggac accggaccaa ttagatggaa tacttgtgac gcatgaacat
     6961 cgagatcata tacatggcgt gggtgtcttg gctcgtaagt atcatttgga tgtttttgcc
     7021 aatcagcaga cttgggaagc gatggaacca ctgattggaa atgtaccagt ggaacaaaaa
     7081 catctatttg agatggggaa agtcagaaca ttcggcgata tcgatattga gagtttcggc
     7141 gtctcccatg acgcagcggc tccgcaattt tatcgtttcc ataaagaaaa tcggtcattc
     7201 gtcatgttga cggacaccgg atattgcagt gattatatgc gaggtgtcat tgaaaatgcc
     7261 gatggttatt tgatggaaag taatcatgat cttgagatgt tacgcatggg accttatccg
     7321 tggaatttaa aacagcgtat tttaggagat agaggacatc tttccaacga agacggtgca
     7381 cttgtcatga ctgatgtgat cggtaatcgg accaaacgaa tctatcttgg ccatcttagc
     7441 aaggaaaaca atatgaaaga acttgctcat ttaacaatgg aaaatgtttt gaaagaaaaa
     7501 gattttggcg ttggacatga ttttgaaatc tatgatacag atccagatga agcaactgag
     7561 ctattttcga tttaaacagt tcaatcaata aaaatataaa aagcagaaga taaaatcaat
     7621 cagggttggt tttatcttct atttttattt ttttagcata ttaaaaaaat aaccgtatta
     7681 tcgactgact acactgatgt gctgcttttc atgtttctca tccagtatct catctactaa
     7741 tgcaatcgcg taatctgcca tgctgatcgt actgtcgcta gcagcgtttc tttgtaaata
     7801 atcatcactt aacaaataag tacctgtacg tttgccatgt ggatcaaata aagaagctgg
     7861 actgatataa gtccaatgta gatttttaga ttgtttcaat tttaacaatc cttcgtacat
     7921 attgaaagct gttggataga agggtgcatt tttaggtact tggtcaatca tacgagtcgt
     7981 cttttctgta tctaagaata acgaagaggc acccccaacg acaattaagc gtgtatcggt
     8041 tccttcaagc atctcaatca aatgggacaa agaagtttga tgcatttctt cttttccttt
     8101 gggagcgttg aaagcatcaa cgacaacatc aaaagatgca agatcagtag ttgtcaactc
     8161 aaataaatcc ttttgtataa acggaacatc tgatggcaat ttttctggat gacgcacgat
     8221 tgccgtcacg tctaaatctc tggacaatgc ttcgtctaaa ataaatgaac ctgcatggcc
     8281 agtagctcca atgagtgcaa ttttcataaa aaacctcctt ttttattcct gtccattgta
     8341 agcagttgct gaatattcgt caaagaatcc gatttttgaa aagctcagtg cgcattcctt
     8401 tcatggttcg tgatcattgg gtatagttaa attaagaaaa ctgattgata aaaatagaag
     8461 ttgaaggaga taagaaatga aagagtggtt agatcgtgcc tttttaccaa tgataacatc
     8521 tgttgtgaat cttagaccct taactgcttt gcgtaatgga atgacttaca cgatcccaat
     8581 aattattgtt ggctcgctgt ttttcttatt tgctcatttt cctgtggatt acatcgctca
     8641 gtggatagac aaaacaggat tttctatgta ttttacacaa gtataccagg ctacatttga
     8701 tgtgatagcc ttttggatcg tttttgccat tgcatacgct tatgcaaaag aagaagacat
     8761 cgatcgctta ccagtcggca taacagcctt gtctgcttat ctactgctta ttcagccact
     8821 aaatactcag atagatactg tctggacagg tgctgaaggg atactaggag cagttacaac
     8881 tgggctcatc atcggatggg gctatgcacg tttagtaaga cttgcgaaag ggtggaagca
     8941 aagagagaag atacctgaaa atgtttggca gtcttttact tcattgattc caatcattgt
     9001 tatagtcact tttacgctta tcggttcagc ggtttttcaa catttgttca gaatgactgt
     9061 tgttcaagga gtatggacat atgtccagct tcccattcaa tcgttcatag acacgttgtt
     9121 tggtgtgctt ttattaggtt tttttgttcc gtttttatgg ttatttggtc ttcatggatc
     9181 tgcgatggtc aatgggttgg ttagtcccgt cttgcaagcg aattcattag caaatgcaga
     9241 aatcttagcc tcaggacaag agttgacggt ggcaaatggc ggacatatcg ttactcagca
     9301 atttctcgat cagttcatga cggttacggg tgctggattg acgttagggg ctgtattttt
     9361 tatgacggtt tttgcgaaat cgaagaaata cagagaatta ggaaatcttg ctcttttacc
     9421 agcctttttc aacatcaatg aatcaattat tttttccaca ccgatcgtga tgaacccgat
     9481 gatggctgtt ccttttattt tcgcaccggt cgtttcagga ttgattacct attccgcttt
     9541 atattttgga ttcgttcctt tatttacagc agtccaagtc ccttggacaa caccgccgat
     9601 cctctcgggt ttcttagtag gcggagtgcc tgcagcgatt ttacaaggta tcgtgttgtc
     9661 aattagtttt ttcatttatt tcccattttt gaaaaaaata gattctgcaa actgcaaaaa
     9721 ggaaagacag acaaaaaatg atggagcagc agaaaagatg aacgactaaa aaacaaaaat
     9781 actcctgtcg ctttagattg ggaaaatcac caatctagga tgacaggagt atttatagtc
     9841 atgtttatac tagccctttt tggacagcta attgaacaag aacaactaat gtgttcattc
     9901 ctacgtgtgt gatcatcgat gtccagatcc gaccagttgc tttgtactct aggctgaaga
     9961 agaaacctag gaaaaagtag attagcagat gaccatcatt atgagcaaac gcaaacagca
    10021 aagagctgat cactgctccg acccaaaaat ttgaatatcg actgatgatc cccagtatac
    10081 tgcggcggaa tacgaactct tccatgatgg gtccagcaac agttgtcgcc aaaataaaag
    10141 cgggtgcttc catgatcatc tggatgatat tttgtgtatt ctcggaaggg gttgctttgc
    10201 caaacaatac agattctaac atgacagcga tgctttgcag aatcaatgcg acaaagattc
    10261 caaccaatcc ataaataaca atctttggag cagaagcttt ttttaccgct gattcgatcg
    10321 ctaagggttc acgttgctta aaataaaaaa tagctaggac aacggcgcct agtatataca
    10381 tgactgcagt tgctgtgatc aattcagacg tagattttac aagtcctatc gaagctagga
    10441 tcaagggtga gataaaaatt agaccatagc aaaaaatgct gaggaagcta tattttttta
    10501 aagacatgta attcctcctt ttaagggata acaagcatgc aaggcaagct tatatcgatt
    10561 gatttcaatt atatcataat ttcaataagc agctgtcaga ataaaatttc ttttctttga
    10621 tttaaaagct tgcaaaaaag agggagaatg agtattatta aaaatgtagg ttagcactcg
    10681 aaggttaaga gtgctaacag attaaaaaaa gagttatcgg agggatttat cgtgttaaaa
    10741 ccattaggtg atcgcgtcat tatcgaagtc gcaaaagaag aagaaaaaac tgtcggagga
    10801 atcgttttag cttctgctgc taaagagaaa ccccaaactg ggacagttgt tgcagtcggt
    10861 gaaggccgtt tgttagaaaa cggtgaaaaa gttcctgccg ctgtaaaagc aggcgaccaa
    10921 gttatgttcg aaaaatacgc cggtacagaa gtaaaatacg aaggaaaaga atatctgatc
    10981 gtcgcaggaa aagatatcat ggcgattgtt gagtaaattt ggaagataaa aaaataatca
    11041 aaaataatca atgaggtgaa tcaatcatgg caaaagaatt gaaatttgca gaagacgcac
    11101 gcgcagctat gttacgcggg gtagataaat tagcagatac agtgaaagta acattaggac
    11161 ctaaaggccg aaatgtcgta ctagaaaaat cttacggctc tccattgatc acaaatgatg
    11221 gtgtgacgat cgcaaaagaa atcgaattag aagatcattt tgaaaacatg ggtgcaaaac
    11281 ttgtatcaga agtagcatca aaaacaaatg atattgcagg agacggtaca acgacagcaa
    11341 ctgttctgac acaagcaatc gttcgcgaag gtttaaaaaa cgtaactgca ggtgctaatc
    11401 ctctaggcat tcgtcgcggg atcgaattgg caacaaaagc agcagtcgaa gaattgcata
    11461 atatctcaac tgttgttgat tcaaaagaag ccattgcaca agtagcagct gtttcttcgg
    11521 gttctgataa agttggacat ttgattgctg atgcaatgga aaaagttgga aacgacggtg
    11581 tcatcacaat cgaagaatca aaagggatcg aaacagaact agatgttgta gaaggtatgc
    11641 agtttgatcg tggttactta tcacaatata tggtaacaga taacgacaaa atggaagctg
    11701 ttctagaaaa cccatatatc ttgatcacag acaaaaaaat ctctaacatc caagatattc
    11761 tgccattgtt agaacaaatc ttgcaacaat cacgtccatt gttgattatt gctgacgatg
    11821 tagatggtga agcattgcca actcttgtat tgaacaaaat ccgtggaaca ttcaacgtag
    11881 tagctgtaaa agctcctggt ttcggcgatc gtcgtaaagc aatgcttgaa gatatcgcta
    11941 ttttgacagg tggtacagta attactgacg atctaggttt agaattgaaa gatgcaacaa
    12001 tcgaaaactt aggaaatgct tctaaagttg ttgtagataa agacaataca acgatcgtag
    12061 aaggttctgg agaaaaagaa gcaatcgaag ctcgtgtgca attgatcaaa aaccaaattg
    12121 ctgaaacaac ttctgatttt gaccgcgaaa aattacaaga acgcttagct aaattagcag
    12181 gtggtgtagc tgttgttaaa gttggtgcag ctactgaaac agaattgaaa gaattaaaat
    12241 tacgcattga agatgcattg aatgctacac gtgccgcagt agaagaagga atggtctcag
    12301 gtggtggtac agctctagtg aacgttatca gcaaagtttc tgctgtagaa gcagaaggag
    12361 acgtggcaac aggtatcaag atcgttgttc gtgctttaga agaaccaatt cgtcaaatcg
    12421 ctgaaaacgc tggttatgaa ggatcagtga ttgttgataa attgaagaac gtggaactag
    12481 gcactggttt caatgctgct actggagaat gggtaaacat ggttgaagca ggtatcgtag
    12541 acccaactaa agtaacacgt tctgccttgc aaaatgccgc ttccgtttct gccttgttat
    12601 taacgacaga agcagtcgtt gcagataaac cagaaccagc agcgccagca gctcctgcaa
    12661 tggatccatc aatgatgggc ggtatgatgt agtaattaag aaagaaccct attttaacag
    12721 ggttctttct tctttttttg ttttatttta agcaaaaggg gcaaagaagg ggcaaaaata
    12781 tcaaaggcta tttagtttat caattacttg ttgtttggct ctttttgtta catggttata
    12841 aatagacaga gttgtgtttg catcagaatg ccctacacgc tccataatgg ctttaagagg
    12901 tacgcctaat tcagataata gtgaaacgtg gctatgtctg aatatatgtg aggatagact
    12961 tttttctaat tccaactctt cctctacttt atggagtata gcgttaaatg agtgcagcgc
    13021 aagcggagtg ccacttgtag atataaatat atattgatcg ggatctgtgg gtctacctgc
    13081 aagaatattg tcagctatca cactttcaat caattctttt gcacgattgg gtaattgcac
    13141 ttcacgttgc gaataagtat tttttggagt tgtttttata gcattatcca ttttcacaga
    13201 tgtgtaatct aaggtcccat taatggaaat tttcccatcc tcatagtcct tcatttgcaa
    13261 agctagcaat tccccatatc tcaaaccagt taaatataga aactcagcta ttatgccgtg
    13321 tagttttctg cgaggattgg agtatagctg tttcagtatt tgatcaattt cttctttctc
    13381 cagatatttt ttattcatag aaagccttct tttttcttct tccacttttt taggatggat
    13441 tttaactgct agcgcagggt ttctttgaat gtattttcta tcgattgcat agtttagcat
    13501 aacagataga gttgtttttg tttgttttgt gtagttcagt gagaggtcac caaacgtata
    13561 catatcttca attatcttat taatgagtgt ctcatcaatg tttctaacga tcgtatcatc
    13621 gcttatgtgc ttagaaacat gtttcatcat cattggaacc ttcaaatagc tagtacgttt
    13681 aacatgctgc ttataatatt cataccattc tttatacagc tcaccaaaag tgatatctga
    13741 tttattgtaa tcttcgagtg cttctttaat ttttttatct agaattttct gagctttttt
    13801 ccacgcttgt ggtgaattac ttgtaagtgt tgtagatttt tttcgtgttt tttctgtata
    13861 aggatctaca tatctttcaa taaacttgaa ttggccatct ttggtctgtt caacccacac
    13921 ttttaacatc tcctatcatt tgctataata ggcataacaa atagacctat ataggtttgt
    13981 tttctaaaag cacgctctta ctctggacgg tggggcgtgt tttttattta ttatacagtt
    14041 gctttaaaaa tagctgcttt ttcaaatgat actaaaggag agaagtggat ttctatttct
    14101 cccttttggt ttaacgtgaa atgagcagtt acatccattt gttttcctgg agcaacagat
    14161 cccattgtat tttcgttggc atatgtctct gattttttgt catctggtcc atatacttcc
    14221 acatctgtac ctacagggat atctgaatcg ccatcatttt ttacggtata agtaattttt
    14281 actacttgtg caggttggtt ttcttcaaat tgatttcttt catcagttag ttctacacta
    14341 tttagtgtat attcagcatc accaactttt acagtgtcac caatcttata gaaggtatcg
    14401 cttttttctt ctttagaaga tgaagcagag gttgaagatt tagttacttt ttcgccacca
    14461 ttatcacttg ctttattact atctgatcca ccatttaaag cagaaccaat aattataatt
    14521 aaaattactg ctaataccca aaaccatact tttttgtaaa aaggtttact aactttatac
    14581 atttttccgt cttgacccat aacttttttt gccatttaaa tattcctcac ttcttgttat
    14641 aatatatttg cgatctcaga aatgaggtat gagtccgtgt gccagcacgg gcttttttaa
    14701 tgttttgctg aaatcggttt ttttaataat tcttgatgta ttcatacata ttgccttgcg
    14761 taagaatatt ttttcttaaa atagcattgg cagacagcat aacaagtgta tctgagctta
    14821 ttctgaatag aataatactc cataaatttt tcgagattaa actgagattc atcagtcagt
    14881 tcgttctcaa tatagatatt taataaaatt agaatagcta ttttatctgc ttctgtttca
    14941 aattttgagt gaaaagttgt agaagtatcg tacaaaactg aatattcaaa atgagaagcg
    15001 gtgaaatgcg caagttcatg agataaatga aaggcttctg cagtttcgcc atatagattt
    15061 tcattcaaaa aaatgattct aggcttcgga taatagaagc caggctcttt catttccatg
    15121 taaactactt tcaaattgta ttcgctcaac atttctttca atttcaaata catacaaccc
    15181 atcactccaa ctattcattt tcctctaaag ctttagcaat tgcaatcgct ttacgcattg
    15241 tctccttaga tatttctttt ccatcaaaag aaaaaacagt atcgttttct gataaatcca
    15301 catgtttagg ggtttctctt tcttctctac ctagaaggta gtctacagag actccaaagt
    15361 aatcagctaa ccgagctaac tcaatagaat taggagtttg tcttttccaa cttcctaaat
    15421 atccattcga atacccgaaa ttcatttcga gctgtctaat ggataatcct ttttcttttg
    15481 ctaactcctt tattatttcg taagtattca ttgataaatc aacctttctg aatgcttaca
    15541 aaaaaagttt agataaataa tgtaaaaata gttgacttat atacattatt aatctatact
    15601 atgttttgta aacaagttta acaactaaaa agacaacaaa aaatactatt gattaataaa
    15661 tgccaaccgc caagaaagct ttttaaatca aagtttatat gtcttattta actatgcatt
    15721 tagtatagat tattaatcta ataaagtcaa cgcagttttt taaaaagttg ttaaatttgt
    15781 ttacgaatat aaaagaaagg agagaagaat atggaaaaaa cagtctcggc aaaaatcgaa
    15841 gatttgaaat tagacatact gaaacaagca aaagtggcga tggaacacgc ggtagataaa
    15901 gaagactctg ccatggttgc agccatagca gagattttag ctcacgttta gtcatcgttt
    15961 ttacggtctt ctggaagctg accatagata acagcataat gaatatctaa ataagcttga
    16021 acaatttctt ttggcgaaat tgattcgcct ttagtagata cttgtgattc gtgatttagc
    16081 catgcaacaa ctaaatccgc agcgatttca aacggcgtta cactttgtgt cagttgccat
    16141 aaagaatttc acaatatgta tggtcgtggt aataatacca aagagcaatt tgaggaattt
    16201 atggaaatta aaaaatacaa aaaatcattt attaaaggag gctaaacaat gaacaaggaa
    16261 atgagccaaa caatcaaggt acaaaaaatg atagatgatc taacacatgg aattgatagc
    16321 caagccgata aaatcatcaa ggaattgcaa ggacaaaaag ttaaggatgc aaaaatgctt
    16381 ctcaagacta tcaactttga aatgaatcca acaaaaagaa agctcgccga tgtgttggaa
    16441 gaaaagttgg cttccgctat caacgagcaa gaattactat ttgaaaccga tacttttaac
    16501 agctgatgtt ttatagatga cagtttcatc cgaatctata tcatcgccga gagaaaagaa
    16561 tgttgctttt gacaaaaagc cgataagacc tacaagcggt cttgatgttg caagagtaga
    16621 taccccaata tcaccttgca aggtattcac gtaaatttga gaagaataga acattccatc
    16681 tttttcttcg atatttgacg catcatcgta tccaacgacc aaagtatcgg caaatatttt
    16741 gattttttct ccatcaagca tagtaattac aaattctggt ttcataaatt ttcacctcct
    16801 taacaattat ttcagcctgt cacactgata aggaaattat accaaagaaa ggaatgagaa
    16861 atatgaatac accacaaatt tttaatttcg agcaaaacga agttcgaact attttagtaa
    16921 atgatgaacc atattttgta ggaaaagatg tagcagacgt tctaggatat tcgaatccgc
    16981 aaaaagctat tcgagatcat gtggacttgg aggataagac gcagaacgat tcgttcaccg
    17041 tcaatggaac agcagttgtt ttgattaacg agtcaggcct ttacagttta atcttaaaat
    17101 caaaacttcc ctctgccaaa aaatttaaac gttgggtaac gagtgaagtc cttccaacaa
    17161 ttagaaaaac aggtagctat tcaaacgtac ctcaaagttt tgcacaagca ttgcgtttag
    17221 cagcggattt agaagaaaag aaccaattac tcgaacaaca aattgccgag tacgaaccaa
    17281 agattagcta cttagatacg attctttcat cgacagatac ggtagctact tctcaaattg
    17341 cagctgatta cggaatgtcg gcaattgctc taaacaaatt gctaaacgag ttaggtgttc
    17401 agcataaagt tagcggacaa tggatacttt accgaaaaca tatgaaccaa ggatacacaa
    17461 aatcgcacac aagtgagata ccgaaagccg atggcggcac taaagttgta atgaatacca
    17521 aatggacaca gaaagggcga gtgtttattt acaacttatt aatcgcagag ggctattacc
    17581 ctcaaatgga tttagaggaa attggttaga aaggagtttt agtatgactg acattgcaga
    17641 aatcactcaa cgagatagag aaaaaatcaa agaatatgtc gaaagttcaa agttcttaac
    17701 ttacaccatg cttgctgaaa gatttggaat tagcaaaagc tacttatctt taattttaaa
    17761 cggtaaaaag acttctgcag aagcaaacag aattatagat tcgattatca ctatgtacga
    17821 attgtagagg gaggtaaagt cgttggaaga taaaatcatt cttacaaaat cagagcttca
    17881 agaaatgtta aacatcgagt atggaacgat aatttttagg agggataata agtgaaaaaa
    17941 ccaacacttt cagagctgat agaagctgct gagaaggcag taaaaccaga cgactggtac
    18001 cgacaaagtt taatcttgga gaagttccac ggcatgtcaa aaactacttt agttgaatac
    18061 tgcaaggaaa tggaaacaat tcctgaattt gcagaaggaa tacttcgtcc aggtcactca
    18121 accacattta ttcattacca tacttttatt tggtttttaa agtggaaaga cgcaaacaaa
    18181 tatcgtgtaa aaagtttatc gccttcagat gttttgaagg aggcaagtta atgggcaaat
    18241 tcaacagagc actagtattc agcgcaccgc taatcatcta cgctttaggc ctctggggaa
    18301 gcagacaagc gttgataggg acaatcgttt acatggtttg gatttttatg gggcttgatg
    18361 aagctgagca cagagcgaaa aaagccagtc gggagggact gactaaaata tgattttttt
    18421 atttttgttc ctttgggctg ctattgcgta cttcatccag ctccttgttg tcaaagttgc
    18481 tcttatttct tttaggaaga acaaaagaaa atagcgctat aaatgaaaga taagtagccg
    18541 ctacattaaa ctgttctttt gaaaaagtta cagtcatcgg aatgataatt gatattgagg
    18601 aagtaatctt aattaaaaat atagcaatat tttcaacatc gcgttggttt ttatttaaca
    18661 ttgtccaaca tgttttagca acaataaaaa taattacaca tgatattagc acacagactg
    18721 aaaaaagttt tagaaatgcg atgtagtcag tgttattgaa ctccttcaaa atactatttt
    18781 tattcaatgt aataaaagga atttttttaa ttgaagacag gaaagataag gtaataaaca
    18841 gaattatata tatggttaaa gtcgcagtaa gaatgtaatt gagctttttc ctcatagaat
    18901 cacctcactt tctaacgaaa attataccaa aaaggagaga agaaataatg caagaattag
    18961 taattttgaa aaataaagaa gctgtaacta cgagcttaca agtggcagaa agtttcaaaa
    19021 agaaacataa gcatgtacta gaagcaattg aatcaataaa aagatcggtc gaaaattcgg
    19081 ccaatgttga agatgggtcc aattttggac agatgtttgt ggaagggaac gagccagact
    19141 catacggaag aagtcggaga gtttatttca tgaatagaga cggattttcc ttgctagcta
    19201 tgggattcac tggaagtaaa gcaataaatt tcaaactaaa atttattgaa gctttcaacg
    19261 aaatggaaga tgttattcgg aagaatactg ttcctcaaac aattgaagac atgatgatct
    19321 atcaattaga agaaatgaaa gatgttaaaa aagatgtttc catgcttaaa gatactatgc
    19381 gaattagcgg acaacaagag tttgaaatta agcaaaaagg aaatatgaaa gttatggaag
    19441 ttctaggggg aaaagaaagc cgagcttatg aagaaatcag caaaaaagta ttctcaaaat
    19501 tttggtctga atttaaacgt accttttcaa tcccaagata tggcgagtta cctcgtaaga
    19561 gattcgatga tgctgtttca tttattgaaa tgtggttacc agaaactgcg atccgcatgg
    19621 aaatcgatca actgaacaga caacaaagac ttttcggtga tgaaaatgaa tagagctgaa
    19681 gcgctaagaa tagggacggt aattgctaat cgctggtgga gacacaataa accaaacatc
    19741 ctaagccaac aacatatcga aaagcaaaaa gcatggcaac aaataaaaaa gtgactcagc
    19801 cggcaagcat agagtcacaa aacatatcta aggagaattt tagcatatga ataaagaact
    19861 ttccacttta gatcaatatt tgactgattc tgaatggggc aagtcgaata tcaaggaaac
    19921 aaataatcga aaaatcagac gaaatctttt gacgaacgaa gaactatcat gtgatcaaga
    19981 tgacttggga aattttgtga gtatttggga tcatgtttac cttatccatc tatcaaaaca
    20041 ttcaaataaa cctgaataca tctatgtcat cgaagatggc ttgattgatg cgctagaaga
    20101 gtacaacaga gataacttga ttgatatctc ttattacgga tcaggtaaga aatacattgc
    20161 tgaaatggag gcagaatttg atgagtgaaa tcaaagggac aacgaacttt gaaaaacttt
    20221 ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa ttttgattat ttatcttggg
    20281 ctcacgcgtg ggagattatg aaaaagaatg atccacaggc aacggtaact attaatgagt
    20341 ataaacacta cagggttgtt tctggaactc atcaagactt tcttgttgag gaatataaac
    20401 cttttcttat ggatgaaaca gggacttatg tatctgtctc agtaacggtt aaaggacata
    20461 cggaaacaga
//
LOCUS       DAZKBH010000042        20216 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000042 DAZKBH010000000
VERSION     DAZKBH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.042"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..1540
                     /locus_tag="ACG8OO_001460"
     CDS             137..1540
                     /locus_tag="ACG8OO_001460"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9045348.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            1540..2973
                     /locus_tag="ACG8OO_001461"
     CDS             1540..2973
                     /locus_tag="ACG8OO_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9045349.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            3118..4044
                     /gene="trxB"
                     /locus_tag="ACG8OO_001462"
     CDS             3118..4044
                     /gene="trxB"
                     /locus_tag="ACG8OO_001462"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9045350.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            4258..5985
                     /locus_tag="ACG8OO_001463"
     CDS             4258..5985
                     /locus_tag="ACG8OO_001463"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFP9045351.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            6000..6350
                     /locus_tag="ACG8OO_001464"
     CDS             6000..6350
                     /locus_tag="ACG8OO_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9045352.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(6442..7302)
                     /locus_tag="ACG8OO_001465"
     CDS             complement(6442..7302)
                     /locus_tag="ACG8OO_001465"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFP9045353.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            7394..7822
                     /locus_tag="ACG8OO_001466"
     CDS             7394..7822
                     /locus_tag="ACG8OO_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9045354.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            7984..8160
                     /gene="rpsU"
                     /locus_tag="ACG8OO_001467"
     CDS             7984..8160
                     /gene="rpsU"
                     /locus_tag="ACG8OO_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9045355.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            8187..8633
                     /locus_tag="ACG8OO_001468"
     CDS             8187..8633
                     /locus_tag="ACG8OO_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFP9045356.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(8787..10166)
                     /gene="brnQ"
                     /locus_tag="ACG8OO_001469"
     CDS             complement(8787..10166)
                     /gene="brnQ"
                     /locus_tag="ACG8OO_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9045357.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            10872..11843
                     /locus_tag="ACG8OO_001470"
     CDS             10872..11843
                     /locus_tag="ACG8OO_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9045358.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            11867..14059
                     /locus_tag="ACG8OO_001471"
     CDS             11867..14059
                     /locus_tag="ACG8OO_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFP9045359.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            14076..14552
                     /gene="ybeY"
                     /locus_tag="ACG8OO_001472"
     CDS             14076..14552
                     /gene="ybeY"
                     /locus_tag="ACG8OO_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9045360.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            14536..14931
                     /locus_tag="ACG8OO_001473"
     CDS             14536..14931
                     /locus_tag="ACG8OO_001473"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9045361.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            14946..15845
                     /gene="era"
                     /locus_tag="ACG8OO_001474"
     CDS             14946..15845
                     /gene="era"
                     /locus_tag="ACG8OO_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9045362.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            15988..16800
                     /gene="recO"
                     /locus_tag="ACG8OO_001475"
     CDS             15988..16800
                     /gene="recO"
                     /locus_tag="ACG8OO_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9045363.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            17184..18101
                     /gene="glyQ"
                     /locus_tag="ACG8OO_001476"
     CDS             17184..18101
                     /gene="glyQ"
                     /locus_tag="ACG8OO_001476"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9045364.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            18103..20178
                     /gene="glyS"
                     /locus_tag="ACG8OO_001477"
     CDS             18103..20178
                     /gene="glyS"
                     /locus_tag="ACG8OO_001477"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9045365.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         6782 a         3580 c         4385 g         5469 t
ORIGIN      
        1 ttttgacaag atatttacaa aaatccacta gaaaacagta aattttgtaa atgttactgg
       61 aaagaaacag atttctattg tttgttttgt ttacaaaaaa gataatgaaa gtgtaaaaaa
      121 gtaaaggagt gagaacatgg caaaaggaat caaaatcgct acgattggtg gaggcagcag
      181 ttacaccccc gagttgatgg aaggatttat caaaagatat gaggaattac cgatacgaga
      241 aatctggctc gtagatgtag aagaaggaaa agaaaagctt gagatcgtta gtcagatgtc
      301 tcagcgtatg tgggatgctt ctccgtatga agtcaagatc tatgcaactc tggatcggga
      361 agaagcattg aaagatgcgg attttgttac gactcagttt cgtgtcggtc aattagatgc
      421 acgaatcaaa gacgagcgga tccctagtta ttatgggatg attgggcaag aaacaaatgg
      481 tgctggcggg atattcaaag cttttcgtac cgttccagtc gttttggcaa tcgttgagga
      541 catgaagcgt ttatgtccaa atgcttggct gatcaatttc gcgaacccaa gtgggatggt
      601 gacagaagca gttatcagat atggcaaatg ggataaagtg attggattat gcaatgtgcc
      661 agtgatggcg atgatgacag aaccagcaat gcttggcaag aaaaaagaag agctgatcta
      721 caagtttgcc ggtctaaatc atttccattg gcacaaagta gcagataata aaggaaatga
      781 ccgcacggca gaattgattg atcgtttata cgatgaagag aatggattac cgaaaaatat
      841 ctttgatatc ccgtttttca aagaacaatt aaaacaaatg aacatgatcc cttgtggcta
      901 ccaccgctat tactatcgtg aagaagaaat gctggaacat gctttagaag aatatcaaac
      961 gattggtaca agagcgcagc aagtgaaaga aacagaagca gagctatttg aattatacaa
     1021 agatccagaa ttagaccata aacctgaaca attgcaaaaa cgaggtggcg cttattattc
     1081 agacgctgcc tgtgaaacga ttgcttcaat ctatggaaat aaagaaacac agatcgtggt
     1141 atctacaaag aatgatggag ctgttcctga cctgccggca gattgtgtag tagaagtcac
     1201 tgcctatgtt ggcgcacaag gagcgagaaa tgtagcgttt ggttctcttc ctgctgctga
     1261 aagaggctgg ttgcaagtaa tgaagaatat ggaattgctg acgatcgaag cagctgtgac
     1321 tggtgattat gggacagctt tacaggcgtt tacgatcaat ccacttatac gatcaggaaa
     1381 gacagcaaaa cggatcatgg atgagttgtt tattgcacac cagcagtatt taccgcaatt
     1441 caaggagaca atcagccgat taaaagaaga aggaatcgaa gttcaagatg aactggcaag
     1501 agaattatca ggtaatacac atataatgga ggaagtataa tgggattcag aaaagatttt
     1561 ttatggggcg gtgctacagc agccaatcaa tgtgagggcg gttatgatga aggtggacgc
     1621 ggactagcaa acgtagatgt tgtgccaatc ggaaaagacc gttttcctgt tatcaccgga
     1681 aagaaaaaaa tgtttgaatt tgatgaggat catttttatc cagcaaaaat tgcaatcgat
     1741 atgtatcatc gttataaaga agatattgct ttgtttgcag aaatggggtt caagacttat
     1801 cgaatgtcca tcgcctggag tcgtattttt ccaatggggg atgagaaaga gccgaatgaa
     1861 gctggcttga aattttatga ggatgccttt aaagaatgcc aaaagcacgg aattgagcca
     1921 cttgtgacga ttacgcattt tgattgtcca atgcatttga tcaaagaata tggtgcgtgg
     1981 cgttccagag aactcgttgg tttttatgaa aatctgtgtc gagtcatctt caatcgctat
     2041 aaaggacttg tcaaatattg gctgacattc aatgagatca atatgatcct tcatgcacca
     2101 ttcatgggcg cagggctgta ttttgaaaaa ggagaaaatg aagaacaaat caaatatcaa
     2161 gcggcacatc atgagttggt tgcaagtgcc atcgcaacga agattgctca tgaagtagat
     2221 ccggagaaca aagtaggctg tatgctggca gcaggagcat attatccata cacttgcaat
     2281 cctgaagatg tttgggcttc tagaaaatca gatcgagaaa attatttctt cattgatgtc
     2341 cagtcacgag gagaatatcc cgcttatgca ttgaaagaaa tggagcgaaa tggcattcag
     2401 atcgaaatgg aaccagggga tgccgcattg ctgaaagaac atactgttga ttttatctct
     2461 ttttcttatt atgcttcccg agtttcgact accgacaacg aactcttgga acagactgca
     2521 ggaaatatct ttgcttctgt caaaaatcca tatctgcaag caagtgaatg ggggtggcaa
     2581 attgatccat taggcttgag aataacgatg aatgatattt atgatcgtta tcaaaaacca
     2641 ttgtttattg tagaaaacgg cttaggtgca gtcgatacgc cagatgaaaa tggttatgtg
     2701 gaagatgatt accgaatcga ttacctggca gcgcatatcc aggcgatgaa ggatgcagta
     2761 gaactagacg gagtcgatct attaggctat acgacttggg gatgtattga tttagtatca
     2821 gctggtaccg gtgagatgaa gaaaaggtac ggattcattt atgtcgatcg tgataaccaa
     2881 ggaaatggca cattagcccg ttcaaagaaa aaatcattca actggtataa aaaagtaatt
     2941 gattcaaatg gagaaaatct ttcgaacgat tgatcaatga tttataaaag tctattgttt
     3001 ttcagcggat ttttcgagta taataaagct attcgagaaa cgcaagcaga acaatagact
     3061 ttttttcgca ttggctgtaa agtatgctta cttgattaag taaaggagaa tctacatatg
     3121 tatgatgtaa tcgttattgg cgcaggtcct gctgggatga ctgctgcttt atatgcttca
     3181 cgttcaaact tatccgtatt gatgatcgaa caaggtgccc ctggcggcca aatgaacaat
     3241 acggcagaaa tcgaaaatta tcctggattc gattcgatca tgggccccga tctagcttat
     3301 aagatgtatg aaagtgtctc aagatttggg acagaaaatg cttatggaat cgttcaagat
     3361 atcaaggatc acggcagcca taaagaagtc atctgtgaag ataaaagtta cgaaggaaaa
     3421 acagtcatta tcgcaacagg atgtatccac cgtaaattag gcgtacctgg cgaggaagaa
     3481 tttgctggac gaggcgtttc ttactgtgca gtctgtgatg gtgcgttttt ccgtaacaaa
     3541 cgtctagtag tcatcggggg tggggactca gcagtagaag aagccatcta tttgacacaa
     3601 tttgcttctg aagtagtgat cgtccaccgt agagatgaat tacgtgcaca aaagatcatc
     3661 caagaccgtg catttgcaaa tgaaaaaatt tcctttatgt gggatacagt cgttgacgaa
     3721 atcgttggaa acgaaatggt agtgacaggt gtgaaagcac gtaatgtcaa aaccggcaat
     3781 gaatttgatt tagcagcaga cggagtattc atctatgttg gactggatcc attaactgca
     3841 ccttttagag aagcaggttt gacgaacgaa gctggctgga ttccaacaga caacgaaatg
     3901 aaaacgaata tgccaggcgt ttttgccatc ggagatgttc gtgaaaaaga tttgcgacaa
     3961 atcactacag ctgttggtga cggcggtatt gctggacaac aagtgtataa atacttggag
     4021 tctttagaag aaacaacagc ataagtttaa aaagagtaga agaacgggtt caattgttct
     4081 tttactcttt tttttcgcgt gaaatttact gaatctgatt tatgcgaaca cgtccaacac
     4141 cttttgataa aagaatcctt tcttgattta agcgtttttt ggtcctcata ctttcatatc
     4201 taataagtga atgctatact tattaagaac catatttcag aaagaggtgt tattttcatg
     4261 tcttgggaac aagtgtatga acaatgggta catgaagaaa atttagaaga aaacttaaaa
     4321 aaacaattaa atgatctggg tgaagatcct gaaaaacttg aagatgcttt ttatgcaccg
     4381 ttggaattcg gtactgcagg tatgcgggga atcttaggac ctgggatcaa tcgaatgaac
     4441 atctatacgg taagacaagc aacagaagga ctagcacgtt tcatggatac acaaggtgaa
     4501 gaaacaaaac gccgaggtgt cgctatcgct tatgactcac gccatcagtc accagaattt
     4561 gcaatggaag cagccaaaac actagcacat cacaatatcc cgtcttatgt attcgaaagt
     4621 ctgcgtccaa caccagagct ttcttttgca gttcgtcatt tgaagacgtt tgcgggaatc
     4681 atggtaaccg catctcacaa cccagcggct tacaatggat ataaagtcta cggagaagat
     4741 ggcggacaaa tgcctcctgc agacgcagat gcattgacga aatatgtccg tgaagtcagc
     4801 aatccgctaa aagtcgaagt attatctgac gaagaagcta aacatagcgg gttgatcacg
     4861 atcatcggag aagaagtgga cgctgcttat ctagaagaaa tcaaagcagt gacgattgat
     4921 cgtgaattgg ttgaaacaat ggggaaagat ttgaaacttg tgtacacgcc acttcatgga
     4981 acagggaaaa tgttgggcga acgtgcgttg aaacaagctg gattcgaaca attcgtacta
     5041 gttcccgaac aagctgtggc agatccagat ttcactacgg tcaaatcgcc aaatccagaa
     5101 gaacattcag cttttgaata tgctatacga cttggagaaa aagaaggagc tgacttgctg
     5161 attgcaacag atcctgatgc agaccgttta ggcgcagcgg ttcgtttgcc agatggttct
     5221 tatgaagtat tgactgggaa ccaaatcgga gcattgtttg ctcaatatat tttggaagca
     5281 catcaatcaa gaggcacttt accagaaaac gcagcggtat tgaaatcaat cgtttccagc
     5341 gaattgccta cagcaattgc ggaacactac ggtgctacca tgttcaatgt gttgacaggg
     5401 tttaaattca ttgctgaaaa aatccaacaa tttgaagaag accattcaca tacttttatg
     5461 tttggattcg aagaaagtta tggttattta gttaaacctt ttgttcgcga taaagacgcg
     5521 atccaagcat tgcttttatt agcagaagta gcagcttact ataaaaaaca aggcaaaacg
     5581 ttgtatgacg gcttgcaaac gatttttgaa acctatggct atttcgcaga aaaaacgatt
     5641 tctgtgacaa tgagcggaca agaaggttct gcgaaaatct ctgctttgat ggaaaaattc
     5701 cgtaaagaag caccgacaga atttgccgga gcaaaagtta ttcaaacaga agactttaaa
     5761 gaattgacga gaacagaccg tgaaggaaac gtagaaaaac taacaacacc gccatccgac
     5821 gtattgaagt atacattgga agaaaatggc tggatcgcta tccgcccttc aggaactgag
     5881 ccgaaaatca agttttatat tggtgtaaaa ggaagctcta cagaagacgc agccgaaaaa
     5941 gtagcctctt atgaagcgat tatcaaaaag atgacagaag aataaaaaac ttgagactga
     6001 tggaaaaagt ggtaaacttt agaagtcagt cttttttcaa gttggaggtg aatggattgc
     6061 cagacacgca aaaatcaaaa gtagagaaag tcagtcaatt attcaaaatg ttgagtgatc
     6121 ccactcgtct taaaattctg ctatatctga aagacgggga acaaaatgtg acagcaatca
     6181 cccaagcagt ggaaatggag cagtctgctg tatcgcatca actacgttta ttaagagaaa
     6241 atcatgtcgt caagtctcat cgcgagggaa aagcgatttt gtatagtctt gatgattctc
     6301 atgtactgga tattttgaac cagacactca aacatgtgga acatcaataa cgataggtga
     6361 aaatagataa acactggagt gtgacaacga agaaatttcg ttgtcacact ccagtgtttt
     6421 ttaagaaaaa gacaaaaatg attaatcgct tttttctgtt gtttcagttc cataataact
     6481 atgatcttct aggtctaaag cattcaaaat aagcgaagcc gtttcttcaa tagataaaga
     6541 tgccacattg atcactacac atcctagttt ttcataaaga tcattcgcaa aatcgagttc
     6601 agctctaatt ttttcgatat cggaataagc agtatcaggg tttaatccgt aagagcgcat
     6661 ccgttcttta cggatattat ttaaaatatc tgggttattc gttagtccca cgattttttt
     6721 aggatcgatt tcccacagct gtttgggaat atgtgcttgg ggaatcaaag gtaaattagc
     6781 aacttttaaa tttttatttg ctaaaaacaa gctcaaaggc gttttggaag ttcgggatac
     6841 gcctagcaaa acgacatctg cttctaaaaa accacgagga tctttcccat catcgtattt
     6901 tacagcaaat tccattgctt tgatccgttt gaaatagttc tcattcaata aatggagggc
     6961 accaggttgc cgaattggct tgataccagt taaacgttca atttctgata tcggtggcgt
     7021 catcacatcg aaactaaata attgatgttc ttcacaaaaa gaacgtgtta tctcgatcag
     7081 ttcttgattg attaatgtat gcaggacaag tgctttttct tctaatgcat cttgcaatgc
     7141 ctgccttaat gttttttcat ctgtcacgaa cgttctgcgg aataggttga actcaacggt
     7201 aggatactga gccatagtag cttgcgctag ttttgtcgca gtttctccag cagagtcgga
     7261 gatgacaaaa aaagtaacga tttctgtaga atcggaagac atggataaaa cctcacttgc
     7321 atttttttta tgtttattat accatactct agaatgcgta cttcgtaatc attttgattt
     7381 ggaggaataa gtcatgaaaa aacagacatt gatcgaacaa tcattagaaa cattgaaaaa
     7441 taacggtttt aaatatacta aaaaaagaga agcattgtta acttatttag tgaagcgtaa
     7501 ccgctacgta tctgctaaag aagtttttga ctatatgaat caggaattca aaggggtaag
     7561 ttatgatacg atttaccgga atctccatga ttttgcagag atcgatttac tggaagaaac
     7621 cgatttaaat ggtgaaatga agtttcgttt tcattgctgt cagggggaaa tcggccatca
     7681 ccatcatttt atctgtacag tgtgcggaac aacgaaagaa atccatatgt gtccaatgaa
     7741 ttatttcgaa gaacagctag gtggatgcac gatcgaaggg catagattcg agattttcgg
     7801 aagatgtgaa aattgtcaat aaggatttaa ataaaataac atttgataat ttaaaaaaaa
     7861 agcaagattt ccttgacggt caaaacagca ttagatataa tatttacagg acagtcttgt
     7921 tttttgagat atatttcaat aacaattacg gttacttttt aagctcggag ggagggaatt
     7981 aacatgtcaa aaacagtcgt tcgcaaaaac gaatcacttg acgatgctct tcgtcgcttc
     8041 aaacgttccg tgtcaaaagc gggtacttta caagaatctc gtaaacgtga attctacgaa
     8101 aaaccaagtg taagacgcaa gaaaaaatct gaagcagcta gaaaacgcaa aaaattctag
     8161 ttctgtgatg aattggagtt ggtagtgtgt cactactaag tagattaaac gatgacatga
     8221 aagcagcgat gaaagcgaaa gataaggaat ctttacaagt catccgaatg atcaagtcat
     8281 ctatacaaaa cgagcagatt aaagaaggtc acgacttgac agaagaagaa gaactaacgg
     8341 tgttgtctcg cgaaatgaaa cagcgacgtg attctttgca tgagtttgaa gaagcaggac
     8401 gtgacgattt agctgaaaaa gtaaaaagtg aaattgtaat agtcgaaaaa tatatgccgg
     8461 aacaactttc cgacgaagaa attcgacaac ttgttcaaga agcaatcact caaactggtg
     8521 cttcatcaat gaaagaattt ggcaaagtta tgggtgcaat tatgcctaaa gtcaaaggaa
     8581 aagcagatgg aaaccaagtg aatgcgattg tgaaagaact attacaatct taattttatc
     8641 atttaaactg cggacagtga ttgtccgcag ttttttgtgt tcgtaaaaaa agaactaaaa
     8701 aacagctaga agaaagaata agattctttc ttctagctgt ttttttcctg tttaatatct
     8761 cttatgttaa attgtgtttg atttggttat tcttctggta gttcatttgc ttcttgttta
     8821 taccctgata ggcgataggc gtaataaaag agtaagctgc cagcgtaacc aagtaacgtt
     8881 gggaccatcc agccaagtcc taatgcagca aaaggaacgt gatgatgata aaatcctagt
     8941 aagcttgcta cgacagtttg ctgactcact aatgcaggcg aatttgccaa catatccaat
     9001 acggctggaa cggcagaaaa gaacatcgtt acttgataga caaatggact cttttggaag
     9061 aattttgctg ttaaggaaag caagattaac gcaagtgaca atggatacaa aagcatcaat
     9121 actggtaaag accattggat gatattatcc aatccggcat tcgcaacgac aaaagagaca
     9181 aaagtcgtta accgcagcca tgtcatataa ctgactttag ggaacagttt atggaaatct
     9241 tgtgcaaaag aaacaacgag tcccatagcg gtagtgaaga cacctaatgt gaccattacc
     9301 cctaagaagc tagcaccgaa gttacctaaa taaaaattag tgatctgaga aagtgcagtt
     9361 ccgccatttt ctgataaagg catcttattc aaactcatcg caccgagaag gaccagacca
     9421 aaataaatca tgacttctaa cgcaacgctc aggctaccag cttttgctgt taattctgtg
     9481 acttttttat ctttaaaacc aagatcacga actgcatgga caaaagtgac acttaaagca
     9541 agtaaagcta ctgcatcaac cgtgttgtat ccttctaaaa cgccatttgt caaagcgttc
     9601 gtctgataag aatctgcagc agattggttt agattcccca tgggttggaa taaagaaacg
     9661 acaaaaatga tagctaataa tgcaagaaac aaggagttta aatactttcc gacatatttg
     9721 accaaacgtg ttggatgtac agtaaggaaa taagcaagta aaaagaaaag agcagagaat
     9781 aaaaacatac ctaatgaatc atatttttca ggcaaaaagg gtttagcagc catttcaaaa
     9841 gcagttgctg ctgtccgcgg tgtaccaaag aatggtccga tcgttaaatg aacaaggatc
     9901 aagaacaagg cagcatacca ttttccgaca ggtttagcta agtcatacag accatcactt
     9961 tttgtcacta ctacagctaa gatagctaat aatgggaaaa gagcgcctga gatggcaaat
    10021 cctacaccag cgctgatcca gtttgatcca gccatttgac ctaagtggac aggaaagatc
    10081 agattacctg acccgaagaa catcccgaag ataagtgaac tgaccatcaa taattgcttc
    10141 catgaataag tttttgtttc ttccataaat ttttcctcca ataattatca ttttgagtaa
    10201 caaaataaac gttgcagaaa ctagaaatgc cactaatatc agattccatg aaaaccacca
    10261 ccttaaaata taaaaaaaag cccttttcca cttgggaaaa gggctctaga ttctagaacg
    10321 gtaccacctt ttttcgctta aaaaagcttc ttcattcacg gacgcttttc gataaagtta
    10381 tccgctagcc tgataatgga ggcagccaca atgatctacg ttttcgacat tgactcggaa
    10441 atgatgttcg tctattcgat ttattgtcat ttctcactta catgactcac tgggaataaa
    10501 tcgctgaata gcctactgtt ttcctcgacg ctttgctgta tatttatttg ttgacgttat
    10561 aatacacctg ttttttttga aatgcaagaa tatttcaaat attttgtttt ctttttttga
    10621 aacaagaaaa atacccagag aatacttgct aagttagacg gaatacttgt cctctatctt
    10681 atgcatagct tacatgatca gcttttttta ttcaaacgag acctttagag aatggaagca
    10741 gctatttttt aacgtttctt ggtacttatg gttaagcaac tcttttagaa ccttttctta
    10801 tttgctatat agtttcttct gagtatggta aaataggaaa gaatgaatcg aaaaaaaggg
    10861 gatgtctatt tttgacggaa gagtctagtt ctttagagat tagattgacg gctgctgatg
    10921 atgtcagcat gcttttaggt gcacacgaca agcatataaa actgattgaa gaaacaacag
    10981 aaacaaccat ccatacacgt ggagaaatca ttcagatcat tggcgaacaa tcccagattt
    11041 ccttggctgc ctcagtcatt cggacattgc aggaattgat caaacgagga atccatgtaa
    11101 gtacaccaga tgtcgtgacg gcactgaaaa tggggcaaaa aggcacatta gattattttg
    11161 ttgaaatgta tgaggaagag atcgtcaaag accgtaatgg aaaaccaata cgggtaaaaa
    11221 acagcggcca aaagaaatat gtcgaatcgg tagccaaaca tgatatcgta tttggcgtag
    11281 gaccagccgg gacaggaaaa acttttcttg cagtagtatt agcaatcgct gccttgaaaa
    11341 aaggtgaagt gcaaaaaatc attttgacgc ggcctgctgt ggaagcaggg gaaaatctag
    11401 gctttcttcc cggggatcta aaagaaaaag tagatcctta tctgcgtcct gtttatgatg
    11461 ctttatacca aatttttgga ttagatcata cgaaccgatt gatggaacga ggcgtgatcg
    11521 aaattgctcc tttagcttac atgcgggggc ggacattgga tgatgcattc gttattttgg
    11581 atgaagcaca gaacacgaca gttgctcaga tgaaaatgtt cctgacacgt cttggttatc
    11641 aatcaaaaat gattgtcaat ggagatacga gtcagattga tctgcctcga gggacaacaa
    11701 gtggtcttgt tcatgcagaa cgaacattaa agcaaatcaa gaaaatcgat tttgttaatt
    11761 ttgaagcaag tgacgttgtt cggcaccctg tagttgccga aatcatcaaa gcttatgaaa
    11821 aggcagatct ccatcaagaa taagctttgc ttaggaggat aagcacatgt tgaataaaac
    11881 actgcagaaa ctacaagaaa aacttggaaa ggcagcattg cctttcctgc tgttcttgtt
    11941 ttttatcatt atctgcggcg ttacatttag tagtgtcaaa caaaaggcca atgatttcag
    12001 agaaggccaa gtagcagaag aaagtatccg tgcgaacaaa acgatcgaga acacggaaga
    12061 aaccgaacaa aaaagaaaac tggcagcaga agcagtcact ccagaataca cttaccaaaa
    12121 agatcttgca gatgaccaga acaatcggat caagcaacta tttgaattga tcgataaaac
    12181 gaatgatgcc atcaacaaat cttatgacga aaagattgat aaggcaaagg aagatgaaaa
    12241 tatcccaaag cctactacag aagagcggat cgccgcgttg aagaaaaatt ttgaaaatgt
    12301 gaatgcagaa aatgttgcgt tttatcaaaa actaccgaac aatttttatt cgacgatatt
    12361 tgaaatgact gaaactgaga tccatactgt gagggatgag agtctgaagc tcattgacga
    12421 acagatgagc aaacaagttc gtgaaagtga attggaagct tttaaacaag aagcagaaga
    12481 gcaaatccaa tatttgaatg tcacaccagc acaacaacaa atgattcgct acttagtgga
    12541 tcaaggtatc gtagtgaatg acgtattgaa tgagaaaaag acagaagaat tgaaacaatc
    12601 cgcacgagaa gctgtacaac cagtgatgat cttccaagga gaaatcattg ttcgtgaagg
    12661 taatcaaatc gatgcttcag ctatgaaaaa attggaatta ctaggactga ctaatcaaac
    12721 aacttcgatt tttccgctaa ttgccatggt tttagctgtt ttgcttcaaa tcggcgtact
    12781 gatttataat tcacttcaat tccaaaatgc aggagaaaga acaaaatacg tgcttttcta
    12841 tgtgacagct atgtcagtca gcatagtgct gatgaaattt ttccaattat tccaaacgga
    12901 acaagctact tatatcccgc tgttttatcc cgctgctttt gcaccacttg ttttaagctt
    12961 tttcttgaac cgaagagcag ggatcatggc tggtattttc caagcagtat ctgcactttt
    13021 cattttttat gattcgattg gcacgaactt tctaacgatt atcttgatgt cttatgtttt
    13081 ctctgggctc atggcaacga ttgtcagacg caaacggatc tcagaacaag gattatctgc
    13141 tgccatgtgg gtcattattt ttcctgtgat gatggatgtc attttgatcg tctatcaagg
    13201 aatgagcttt ggtgatgcaa cgacttggtt aatgcttgtc tgcgggttca ctggtgcact
    13261 cttctcattc ttgctgacga tcggtctaca cccatatatt gaattgatgg tcaatgatga
    13321 cagtgtaatt gtattaaatg aactcagcaa tccaaatcat cctttattga aacagttgct
    13381 ggaagaagcg ccaggtactt atcatcatag tatgatggta gctaatctaa gtgctaacgc
    13441 tgtagcagaa atcggcggac gttctttgct aactcgagta gcttgctatt atcatgatat
    13501 cggcaagatc aaacatgcta gctttttcgt tgagaacttg ccttcaggag cggaaaaccc
    13561 gcacaatttc ttattgccag aagacagtaa acatattatc tttggacatg tgacagatgg
    13621 agccaaaata ttggaagatt ataatatgcc gcaaatggtg atcgatattt gcaaacagca
    13681 tcatggaacc acgctaatgc gttatttcta catcaaagcg aaagaacgca atccggaagt
    13741 aacagaagag caattccgtt atcctggacc aaaaccgcaa agtcgtgaag cagggatcgt
    13801 caatattgct gacagttgtg aggctgcagt acgggcaatg gatcatccga cgagcgaaaa
    13861 aatcgaaagt tttgtccata atcttatcga agagcgtatt tccgatggtc aattagacga
    13921 tagtggactg accttgaaag aaatccgaaa agtggaaaaa tcattgatca gcggtttaag
    13981 tagcaccttc cattctcgga tcaaatatcc taaaatgaaa acagaagttg agaagataaa
    14041 ggaagaacaa gaagaataga aagaagcgat gtataatgga tattaccttt atggatgaaa
    14101 caaaccaagt ctcagaagag aaaatgaaag aaatcgatga cttgctgcaa tttgcagcat
    14161 cttatttaaa gctgccagag gaaacagaaa tgtctgttac tttcatggac aatgcggcaa
    14221 tccaagtaat caatcgtgat taccgtggaa aagatgcacc gacagacgtc atcagttttg
    14281 ctctggaaga agaaggcgaa gacgaaatcc cagtgatttt tgaagatgga gagaatccat
    14341 tgcctcgtgt actaggagac atcatgattt ctattgaacg tgctgctgaa caagcagaag
    14401 cttacggcca ttcatatgat cgggaacttg gctttttagc ggtacatgga ttcttgcata
    14461 tcaacgggta tgaccatatg acaccagaag atgagaaaga aatgtttggt ttacagaaag
    14521 agatcttgga tgcctatgga ctcaaaagat aaaaacgtag agaaaaacaa acatttcctg
    14581 atgtctgtgg aatttgctgt ccaaggagtc aaaaccgttt ttgaggaaga acgcaatatg
    14641 aggaaacatg ttgcgttcgg tatccttgct ttatttatgg gcgtgatttt ccagcttgat
    14701 cggatagagt ggttatggct gcttttagct gtttttctcg tttggatagt agagatcatc
    14761 aatactgttt ttgaaaatgt agtggacatg tttacggatt ttcattttca tccgattggg
    14821 aaaaaaatca aagatatggc agctggagca gttttagtga ccgcttgttt tgctgtcatc
    14881 attgggttga ttttatttgt ccctaaaata tatcatttgt tgtttaatta aaaaggagaa
    14941 cgtatatgcc agaacataaa tcagggtttg tcgcaatcgt cggccgcccg aatgtaggaa
    15001 aatccacatt attgaaccga atcgtgggtc aaaaaattgc cattatgagt gacaaggcac
    15061 agacaactag aaataaaatc caaggagtat atactacacc agaagctcag ctgattttta
    15121 tcgatacacc aggaatccat aaaccaaagc atcgtctagg agattttatg gtagaagcag
    15181 cttacagtgc gttgaaagaa gtggatgcag tcttatttat gatcagtgct gatcaaaaac
    15241 gcggccgggg agacgatttt attattgaac gtctaaaaaa cgtgcattct ccagtttatc
    15301 tgattatcaa taaaatcgat aagatccatc cagacgagtt gctgggaatc atcgaagatt
    15361 attcgacgca gatgcctttt gcacaagtga ttccgatctc tgctacagaa ggaaacaatg
    15421 tcgaacgctt aatggatgta ttagtcagtg aaatgccaga gggcccacaa tattttccag
    15481 atgatcaagt gactgatcat cctgaatact ttatcgtttc tgaattgatc cgtgagaaag
    15541 tcttgttttt aacacgagac gaagtgcctc attctgtcgc agtcgttgtt gattcgatga
    15601 agcgaaacga gaatgataaa atccatatcc aggccacgat catcgtcgaa agagatagcc
    15661 aaaaaggaat cattatcggc aaaggcggaa aaatgctgaa gcaagtagga acgaaagcaa
    15721 gaaaggatat cgaaaatctt ttaggagaca aagtatttct agaactttgg gtcaaagtcc
    15781 aaaaagactg gcgagacaaa aaagtctatc tgcaagactt tggctaccga aaagacgaat
    15841 actgatattc tgaacttctt ataggttaag tatagaaata aactggataa aaatccgcac
    15901 gttgagaaaa acggcggaag atcgaaacat aaataatctg gatcggcaga atctgctttt
    15961 ccagatttat tttttaggag ggagaatatg agccagcttg cagaatcgaa aggcttgatt
    16021 ttatctagcc gtaattacaa agaaaaagat aaattagtca aaatctttac ggaatcttct
    16081 ggcaaaatga tgttttatat caaaggaatc catcggcaaa atcagccatt agctccggct
    16141 ttgctcccct ttaccgaagc agtctatatc ggtaattttc gaagtgaagg tttgtcgttt
    16201 ttaagcagtg tcaaagacgt ccatgcgttt cggaacatcc aagaagatat ctttttgagc
    16261 ggttatggta cctatttgct aaatctagta gatgcagcaa tcgaagatca tcaatatgac
    16321 ccgcatttat tccaatttac gcaacaagct ctgcagcgga tggatcaagg agatgatgca
    16381 gaaatcatca cgaatatctt tgaagttcaa atactgcaac gcttcggaat cgcgccaatt
    16441 tggacgcact gtgtcgtatg cggggaaaca aaaggaaaat ttgactattc ttccaaatct
    16501 ggcggtgtga tatgcgagaa acactggcca atggacgaac accgttatca tgcagatcct
    16561 cgcgctgttt atttcgttcg aatgttttcg gctatttctt atgataagat cagtggaatc
    16621 cacttgaaag aagaaacgaa gcaggcaatc cgtgacctca ttgaccagtt gtatgatgaa
    16681 tatgtcggta ttcatctaaa aagcaaaaag ttcattgatc aaatgaagtc ttgggcacac
    16741 gtattaaaga cgccagagaa aaatatgcct gaaaaagaga tacctgaaaa agaagaataa
    16801 atgaaagata caacaaaaaa atcaaaacta tttgacaaat acagggactt ccgtctatta
    16861 ttaaccgtag attaaataga tcgttcactg agaggatcta ttatataaac aagaaaactg
    16921 caaaatgaat acgtacattg accaagatca gtaaaaaaca cgtggtttag cgagttcggg
    16981 agagtggaag ccggacccaa acgatttttg aagaggcact tgcgagtacg ataaatgaag
    17041 ctgccgaatc tttattcgga agtagggtgg aaccgcgaaa ttattcgtcc ctatgtctgt
    17101 aatcagacat agggactttt tgtcgttttc tttcctgtat tcttattgac agcaacgctt
    17161 aaaaattgaa aggggcaaaa cagatgagta aaaaattgac cgtccaagat atgattttaa
    17221 cattacaaaa attttggtca tcaaacggct gcatgctgat gcaagcttat gacacagaaa
    17281 aaggagcagg tacgatgagc ccatatacat tcttacgggc aatcggaccg gaaccatgga
    17341 atgctgctta tgtagagcca tcccgccgac cagctgacgg gcgttatgga gaaaacccaa
    17401 accgtttgta tcagcatcat caatttcaag tcgtaatgaa accttcacct gaaaatatcc
    17461 aagagcttta tttagaaagc ttgcgtcttt taggaattga tccattagaa cacgatattc
    17521 gttttgttga agacaactgg gagaacccat cgatggggtg tgccggtgtg ggttgggaag
    17581 tatggcttga tgggatggag atcacacaat tcacgtactt ccagcaagtc ggaggactac
    17641 cttgtaaacc agttacttct gagatcactt atggactaga acgtcttgct tcatatattc
    17701 aagaagttga aagtgtctat gatctggaat gggctgatgg tgtgaaatat ggcgagatct
    17761 tcaaacaacc agaatatgaa cattccaaat attcttttga agtaagtgac caagacttgc
    17821 tactaagcaa ctttgaccgt tttgaaaaag aagctaaacg ctgtatcgat gaaaatcttg
    17881 ttcatccagc gtatgattat attttgaaat gcagccatac attcaattta ttagatgctc
    17941 gcggtgcagt atccgtaaca gaacgagcag gttatctttc ccgtatccgc aacatggctc
    18001 gtgctgtagc caaaatcttt gtggcagaac gcgaaaaatt aggattccct ttattgaata
    18061 aaaacacagc aaatccagaa aacatgaagg aggctgaata agatggcaaa agacttatta
    18121 cttgaaatcg ggctggaaga aatgcctgct catgttgtca caccaagcat gaaacaatta
    18181 gaagaaaaaa cagcaaaatt tttagatgaa aaccaattga cttatgatac gatcgaaacg
    18241 ttttctacac cgcgccgctt agcaatcaaa gtaacaaaca ttccagaacg tcaagcagac
    18301 agtgaagaag aagtcaaagg accagccaaa aaaattgctt tggacgcaga tggaaactgg
    18361 acgaaagcag ctgaaggatt tgtccgcgga caaggtctga cgacagatgc tatcactttt
    18421 agagaattaa aaggcgtgga atatgtctat gtaacgaaat acactcacgg caaagaagcg
    18481 aaagaagtat tgacaggttt agcagatgtc attaaaagcc tcactttccc agtaacgatg
    18541 cattgggcag aatacgattt tgaatatatc cgccctattc actggatcgt ggcattgtta
    18601 gatgacgaag taatcccgtt tgagatctta gatgtcaaaa caggcagagc ctctcgagga
    18661 catcgtttct taggcgaaga tgtgacattt gcgcatgcaa aagaatatga agaaaaattg
    18721 aatgaacaat tcgttattgc ttcaccgcaa aaaagacaag aaatgatcgt tacacaagca
    18781 aatgaattag ccgaaaaaaa tcactgggtt attgatctag acgaagattt actagaagaa
    18841 gtcaacaatt tagtcgaata tccaactgtc ttttctggcg acttcgaaga aaaatatctt
    18901 tctgttcctg aagaagtact agtcacttct atgaaagaac atcaacgtta tttcgaggta
    18961 cggaacgatc aaggtatgct gcttcctcat tttatttctg ctcgtaatgg tgatcgggta
    19021 aaattggaaa atgtggttcg aggcaatcaa aaagtattga cggctcgttt agaagatgcg
    19081 gaattctttt ataatgaaga caagaaagct tcaatcgacc aatacgttga aaaactaaaa
    19141 actgttactt tccatgaaaa aatcggtagt atctatgaga aaatgcagcg ggtacaagtc
    19201 attgcccgta tcattggcga acaagtagga ctatctgagg aagaattgat ggatctgcaa
    19261 cgagctgctg aaatctataa atttgatcta gtaaccaaca tggtagggga atttcctgaa
    19321 ctacaaggga tcatgggcga aaaatatgct ctgctgaaag gcgagaaacc tgctgtagca
    19381 acagcgattc gtgagcatta catgcctata tccagtgaag gagagctacc tcagtctaca
    19441 gtaggttctg ttttagcgat tgcagataaa ttagatagca ttttttcttt ctttgcagtt
    19501 gggatgattc caagcgggtc aaacgatcct tatgccttac gtcgccaagc atacggtgtc
    19561 gttcggatca tcgaatcgaa aaaatgggcc tttcctttgt ctgttctgca agaaacgatt
    19621 agtgatgtta tcagtaaaga tacggatcgt ttcggcattg gtttatcaga agggcaacaa
    19681 caggtgatcg actttatcaa aggccgtctg cgccaattat tgacaacgaa gaatattcgt
    19741 catgatgtca tcgaagctgt tttgaatgca gaacaaaaag atctgacaaa agtatttgca
    19801 gcagcacaat tgttcaagca gcatcttgca gatgaagact tcaaaccatc gatggaagct
    19861 ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg aaaaacaatc agaagtagac
    19921 cctgaactgt tcgaaaatga agcggagaaa gaactgcaca aagcagtaga ggcaatgaaa
    19981 aaaagctttg ctgaccaaac gatcaacgaa aattatgatc agcttgtaag ccttcgccca
    20041 ctgatcgaaa attattttga tcagacgatg gtcatggcag aagaaccagc agtacgtaag
    20101 aaccgattaa cgcaattaag tcaaattgct gccatggctt tgtcgctagc aagtttagat
    20161 caaatcatca caaaataatt tctaatatgt aaataaacca gagaataggc acaaaa
//
LOCUS       DAZKBH010000043        19841 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000043 DAZKBH010000000
VERSION     DAZKBH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.043"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1077
                     /locus_tag="ACG8OO_001478"
     CDS             202..1077
                     /locus_tag="ACG8OO_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP9045366.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ACG8OO_001479"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ACG8OO_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ACG8OO_001480"
     CDS             complement(1642..2973)
                     /locus_tag="ACG8OO_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9045367.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="ACG8OO_001481"
     CDS             complement(3122..3835)
                     /locus_tag="ACG8OO_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9045368.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="ACG8OO_001482"
     CDS             4010..5434
                     /locus_tag="ACG8OO_001482"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9045369.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="ACG8OO_001483"
     CDS             5733..6440
                     /locus_tag="ACG8OO_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9045370.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="ACG8OO_001484"
     CDS             6427..6750
                     /locus_tag="ACG8OO_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9045371.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="ACG8OO_001485"
     CDS             complement(6839..7876)
                     /locus_tag="ACG8OO_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9045372.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="ACG8OO_001486"
     CDS             complement(8197..9180)
                     /locus_tag="ACG8OO_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFP9045373.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="ACG8OO_001487"
     CDS             complement(9595..10365)
                     /locus_tag="ACG8OO_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9045374.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="ACG8OO_001488"
     CDS             complement(10358..11293)
                     /locus_tag="ACG8OO_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9045375.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="ACG8OO_001489"
     CDS             complement(11609..13258)
                     /locus_tag="ACG8OO_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9045376.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="ACG8OO_001490"
     CDS             complement(13375..15978)
                     /locus_tag="ACG8OO_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP9045377.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="ACG8OO_001491"
     CDS             complement(15990..17039)
                     /locus_tag="ACG8OO_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9045378.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="ACG8OO_001492"
     CDS             complement(17065..17379)
                     /locus_tag="ACG8OO_001492"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP9045379.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="ACG8OO_001493"
     CDS             complement(17395..17841)
                     /locus_tag="ACG8OO_001493"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFP9045380.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="ACG8OO_001494"
     CDS             complement(17842..19764)
                     /locus_tag="ACG8OO_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9045381.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         6065 a         3963 c         3226 g         6587 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa t
//
LOCUS       DAZKBH010000044        19455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000044 DAZKBH010000000
VERSION     DAZKBH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.044"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3658
                     /locus_tag="ACG8OO_001495"
     CDS             131..3658
                     /locus_tag="ACG8OO_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFP9045382.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="ACG8OO_001496"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="ACG8OO_001496"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFP9045383.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="ACG8OO_001497"
     CDS             7446..7787
                     /locus_tag="ACG8OO_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9045384.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="ACG8OO_001498"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="ACG8OO_001498"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9045385.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /gene="pheT"
                     /locus_tag="ACG8OO_001499"
     CDS             9144..11564
                     /gene="pheT"
                     /locus_tag="ACG8OO_001499"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9045386.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG8OO_001500"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG8OO_001500"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9045387.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="ACG8OO_001501"
     CDS             12998..13735
                     /locus_tag="ACG8OO_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9045388.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="ACG8OO_001502"
     CDS             13749..14564
                     /locus_tag="ACG8OO_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9045389.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="ACG8OO_001503"
     CDS             14577..15224
                     /locus_tag="ACG8OO_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9045390.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="ACG8OO_001504"
     CDS             15240..15896
                     /locus_tag="ACG8OO_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9045391.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="ACG8OO_001505"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="ACG8OO_001505"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9045392.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="ACG8OO_001506"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="ACG8OO_001506"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9045393.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="ACG8OO_001507"
     CDS             18238..18756
                     /locus_tag="ACG8OO_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9045394.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /gene="cbpB"
                     /locus_tag="ACG8OO_001508"
     CDS             18863..19363
                     /gene="cbpB"
                     /locus_tag="ACG8OO_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFP9045395.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6540 a         3341 c         4179 g         5395 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggagaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagc
//
LOCUS       DAZKBH010000045        19126 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000045 DAZKBH010000000
VERSION     DAZKBH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.045"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..703)
                     /locus_tag="ACG8OO_001509"
     CDS             complement(<1..703)
                     /locus_tag="ACG8OO_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9045396.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="ACG8OO_001510"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="ACG8OO_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="ACG8OO_001511"
     CDS             1758..2837
                     /locus_tag="ACG8OO_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9045397.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2834..3462
                     /locus_tag="ACG8OO_001512"
                     /pseudo
     CDS             2834..3462
                     /locus_tag="ACG8OO_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3572..3799)
                     /locus_tag="ACG8OO_001513"
     CDS             complement(3572..3799)
                     /locus_tag="ACG8OO_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9045398.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="ACG8OO_001514"
     CDS             complement(3902..6490)
                     /locus_tag="ACG8OO_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFP9045399.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="ACG8OO_001515"
     CDS             complement(6510..7169)
                     /locus_tag="ACG8OO_001515"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9045400.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACG8OO_001516"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACG8OO_001516"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9045401.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="ACG8OO_001517"
     CDS             complement(7942..8808)
                     /locus_tag="ACG8OO_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9045402.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="ACG8OO_001518"
     CDS             8917..9558
                     /locus_tag="ACG8OO_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9045403.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACG8OO_001519"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACG8OO_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFP9045404.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="ACG8OO_001520"
     CDS             complement(10999..11898)
                     /locus_tag="ACG8OO_001520"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9045405.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="ACG8OO_001521"
     CDS             complement(11900..12697)
                     /locus_tag="ACG8OO_001521"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFP9045406.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="ACG8OO_001522"
     CDS             complement(12675..13379)
                     /locus_tag="ACG8OO_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFP9045407.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="ACG8OO_001523"
     CDS             13510..14085
                     /locus_tag="ACG8OO_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFP9045408.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="ACG8OO_001524"
     CDS             14203..14880
                     /locus_tag="ACG8OO_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFP9045409.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACG8OO_001525"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACG8OO_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFP9045410.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="ACG8OO_001526"
     CDS             complement(16943..17941)
                     /locus_tag="ACG8OO_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFP9045411.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="ACG8OO_001527"
     CDS             complement(18167..18820)
                     /locus_tag="ACG8OO_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFP9045412.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DAZKBH010000046        19045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000046 DAZKBH010000000
VERSION     DAZKBH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.046"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..744
                     /locus_tag="ACG8OO_001528"
     CDS             88..744
                     /locus_tag="ACG8OO_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9045413.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(1186..1983)
                     /locus_tag="ACG8OO_001529"
     CDS             complement(1186..1983)
                     /locus_tag="ACG8OO_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFP9045414.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1980..3164)
                     /locus_tag="ACG8OO_001530"
     CDS             complement(1980..3164)
                     /locus_tag="ACG8OO_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFP9045415.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3189..4436)
                     /locus_tag="ACG8OO_001531"
     CDS             complement(3189..4436)
                     /locus_tag="ACG8OO_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9045416.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            4651..6396
                     /gene="dnaX"
                     /locus_tag="ACG8OO_001532"
     CDS             4651..6396
                     /gene="dnaX"
                     /locus_tag="ACG8OO_001532"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9045417.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6416..6730
                     /locus_tag="ACG8OO_001533"
     CDS             6416..6730
                     /locus_tag="ACG8OO_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9045418.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            6755..7351
                     /gene="recR"
                     /locus_tag="ACG8OO_001534"
     CDS             6755..7351
                     /gene="recR"
                     /locus_tag="ACG8OO_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9045419.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7415..8059
                     /gene="tmk"
                     /locus_tag="ACG8OO_001535"
     CDS             7415..8059
                     /gene="tmk"
                     /locus_tag="ACG8OO_001535"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9045420.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8074..8403
                     /locus_tag="ACG8OO_001536"
     CDS             8074..8403
                     /locus_tag="ACG8OO_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFP9045421.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8417..9355
                     /gene="holB"
                     /locus_tag="ACG8OO_001537"
     CDS             8417..9355
                     /gene="holB"
                     /locus_tag="ACG8OO_001537"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9045422.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9391..10215
                     /locus_tag="ACG8OO_001538"
     CDS             9391..10215
                     /locus_tag="ACG8OO_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFP9045423.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10208..10555
                     /locus_tag="ACG8OO_001539"
     CDS             10208..10555
                     /locus_tag="ACG8OO_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFP9045424.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            10624..11496
                     /gene="rsmI"
                     /locus_tag="ACG8OO_001540"
     CDS             10624..11496
                     /gene="rsmI"
                     /locus_tag="ACG8OO_001540"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9045425.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11605..12726
                     /gene="dinB"
                     /locus_tag="ACG8OO_001541"
     CDS             11605..12726
                     /gene="dinB"
                     /locus_tag="ACG8OO_001541"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9045426.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(12780..13382)
                     /gene="nrdG"
                     /locus_tag="ACG8OO_001542"
     CDS             complement(12780..13382)
                     /gene="nrdG"
                     /locus_tag="ACG8OO_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFP9045427.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(13697..15886)
                     /gene="nrdD"
                     /locus_tag="ACG8OO_001543"
     CDS             complement(13697..15886)
                     /gene="nrdD"
                     /locus_tag="ACG8OO_001543"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9045428.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16212..16979
                     /locus_tag="ACG8OO_001544"
     CDS             16212..16979
                     /locus_tag="ACG8OO_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9045429.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16969..19011
                     /locus_tag="ACG8OO_001545"
     CDS             16969..19011
                     /locus_tag="ACG8OO_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9045430.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         5963 a         3470 c         3960 g         5652 t
ORIGIN      
        1 tatcattagt aaaatacctt tggactttaa aaagttttaa agtactgtat gatcttttta
       61 gatgaaagtt tggatggggg agcatcattg gatagtagag tcttaaagga atatttggat
      121 aaaaatgatt ttccgattat acgcaaagga tatcataaat atttgacatt cgaagggatg
      181 gaagattccc atacgtatat cttgaaagag ggaacggtaa aagcaagtgt tatttcaaat
      241 gatgggagag aatttaacct tcgctatatc aaagatcttg aaatcgtctc tcttttaaaa
      301 gacgaatatt cacaattcat cgattcgccg tataatattc gagttgaatc agaatatgca
      361 gagttttatc gaatcaatcg tgtgcgcttt tggcaaaata tcaaccaaga cccaaagcta
      421 caacactatg taaaagagta ttaccgctat cgtctgcttt attcgatgaa aaaaatgcag
      481 caaatgctat taaacggaaa gctgggagct gtttgtacgc aactttacga attatatgat
      541 ttattcggtg tgcggacaga agaagggatg ctgatcgatt tttcgatcac gaatgaagaa
      601 atcgccaaat tttgtggcat ctcaactgct agcagtgtaa acagaatttt aaaacaactg
      661 aaaaaagatg gtattatcac cgttaaagac catcatatcg ttattttgaa tatggacttg
      721 ttagaagaca atattatctt ttgatgaaca aagaggttgt gaaatcagcg ttttgacctg
      781 agtattagag aaacaaacgg aaaaatagtt cctcatattt tgccgttttt tgagctaata
      841 ccgagggtca gctgattgaa caccgtttac caagaggttg tgagcctgcc gtttaactct
      901 gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat tgtgacttgt
      961 gaccgaggag ttggtaggag aacaccgttt accaagaggt tgtgaaaaag ctgttctgca
     1021 tcaagtaata agaagagcca agtaaaaaca gtctcttatg tttttaactt ggctggactt
     1081 atcaccgtag atgtaagctt ttgaacgccg tttatttgca acaaaagggg ctgtgacacg
     1141 atttttgtca cagccctttt tttgcgaaca ttcatttagc aatttttaga aacttctgcc
     1201 gccaccgcca aaagtcccgc caccgctaga atgggtagta cttccgccac cgccgcttcc
     1261 aggaggtggg cttttgggga ttcttcttgt cgtgacaaaa gaatttgtca gacgatcggt
     1321 tttttcagtt aatttgatac tggatttttc tcggaatgga tatttgtaga tgcctgattt
     1381 caattgataa cgcgaaacag tcacaaaata aaagacaaga ctaaggataa gagccaaaac
     1441 aacagcaatg atgatttcta ctgtcgtcaa aaccttatag cgtgtaattt tgccagtttc
     1501 ttcgtctaca cgataatgtc ctcctggaac tccgtcttcc acatatgttg ctgatttaga
     1561 caggtaagct tttgcagcat tgtaataggc agcatttgtc atctgatcat aaacatcatc
     1621 taagatcgaa tcgattctgc tatcagtcaa ataatcgatc atattaccag aagtcgaaat
     1681 gtagatttca cgctgtccca tatctaatag caatactgag ccattttgat catttccgac
     1741 tgcgtctctt aagtaattat cagcaaagtc tcttggttct tctgaattag atgtagtcgt
     1801 tacgatcaag actcgtcctt tgattttttc attcagcttt tctgcctgtt gttccaaaga
     1861 ttggatttga tctgttgtga ataacccagc ctcatcattt accgtagctg tatctgcatg
     1921 agtagccacc ggccagccaa cacttactag caaaagaaag aataaaaaaa actttttcat
     1981 catatcaagt aacctccgat cagaaagagg atcgctagcc cagcgaaaat cccaagagcg
     2041 gtcagtccca attttttctg gctgatcggc aggacaccgc tgacctttcc tgtttgtcca
     2101 ttcattgcgt agtagtagac ttttttactt gagtcattgc ttcgataagt aacaagccat
     2161 accggcagta atacgtaatt atttacttcg tttgtgattg aagcagaagt tcgcacaccg
     2221 gtcaatgttg tatatccatt tgcggtatct tttaacagct tctctgaata gtcttttaat
     2281 tcagattgaa tctgtgattt gattgcctca tattcgatat ctcttttttc tgcttggaac
     2341 ccggctaaat actggctttt aaaatcaatt gctttatcta acgggaaagg ttgcacgcct
     2401 tcgaccatct tttgttgcgt attttttgat aaggcatttt ttactagttc tttaaaagaa
     2461 agcttgcctg ctcttgaaac agcaaattgc ttcgtttctg tgtattcgat gtcacctatc
     2521 cgccatatgc ggataactgt accattcgcc tgcatggcac catctacttc agcatctgta
     2581 gcccaataag ggaaatacac acctgtcaac ttatcgattt gatccttatt gaaaaaagct
     2641 ttgggaacaa accatttttt cttggtccaa gcgagaaatc tgttgattgc ttcttctttc
     2701 tcgacagcaa aaggaagtac tttatttggc aagaattctc cgcttaaacg tcccgatagt
     2761 acgacgggat tatggcagta gtaacaataa gtcgcagcag ttgtcgcatc agtcacgatc
     2821 tctgcaccac agttgggaca aagaaaaaga tctatcgctg cggtattatc tgcagattcg
     2881 tccgctgtat ctgaaaaacc gcctgcctcg tcaaccgctt tgcgctcggc ttcattcata
     2941 tcggcaagct gttcttgggc tgtaaaagta aaatcttcgg taggtgaagg ttcttctttg
     3001 ctttcttcgc cagccatacg cgcttctttt tgcttttgct catattggct gacttcttct
     3061 tctgtataga tattcaggca gtattcgcaa tgaaattttt ggtctttggg atcaaaagtc
     3121 aacggaccgc cgcaattagg acatttatgt gtaagaacat ccatcagctg cacacctttc
     3181 taaataaatt aattaacggg gattccttta aacggtttac cacagtttgg acagaattta
     3241 gggatgccgt tggaaagatc cacgatctca ttgcattcac tgcatcgcat ttgtagctta
     3301 ggctgaacgt tttctacagg tttggcagct ccgcagttgg aacagaattt tccggaattt
     3361 tctgtcccgc aaactggaca tgtccaagta tccgcatttc cttgtgcagt ttgttgattt
     3421 tgtttttctg cttgttgttt gatttgttct tggttatttt gagctgcttg agaaagataa
     3481 gaaccatttg cattcattcc catacctaca cccataaagc cggtcattgc tcctgcttca
     3541 ttttttccgg ctgcttccat accacgtgca atggaacctt gcacgtatcc ttcacgtacg
     3601 ctaggatcac ctagcatcgc cccttcgtta cgcatattga tcagtttcac agaatcatcg
     3661 gtgtaagaaa tgctggctac agccacagaa acaatctcca tcccacgtaa ttcacgccaa
     3721 gaatcatcca atgcagtatc catgtatttg cttaattcca aactcttaga tggtacataa
     3781 gaaatccgtt gaccatctgc agacatctga ttgattgctg attgtagcgc tgttaggaat
     3841 tcagctaaat actgctcatt gatatcattg atttccactt gtgttttgtt tttagggatt
     3901 gcgtttgtat aaaataaaat cgggtcaacg acatgaatag aataagtgcc atgtgcccgt
     3961 aagaataatt ctgcgttata gaaattgtca aagtagttca acggcgcgct tgtaccgaat
     4021 ttgattccct ttatttcttg gagattgata taaaaaactt gttgtttctg cggtgtgacg
     4081 ccgccaaatt tgaatcggtc aaaagtttca gcaattgctt ctttcagtgt tccattgaac
     4141 atggaaggag ctgcgtcatt tttaatggtg taataacctt cctcagcggt ataatcgatg
     4201 atttttccac catcgaccaa aagcatcatc atgtttggat agacatggac gactgttccg
     4261 tctgttaaaa catcttctgt tcctttacga ttggagccac gtttgtcatc tttgcggact
     4321 ttgacacctt ttgtcatgac cgttgtatca ctcatattgt ctggttcgat gacttctagc
     4381 cattgatctg ccagaccgcc gccaaccata cttgttgctg ctttgataag acccataaaa
     4441 aattcctcct ctgattcagc tagtgccaaa caattattgt ttatttatta taccatagga
     4501 agatgtgaaa cttctcggac tagagacgga attgaaatgg aaaattgcgt attgtttaaa
     4561 tacctgaaaa agccgagaag gatagtagtt tttttaccta atatcacgta taataagaga
     4621 tagtgaattt tttaaaaaag ggggatcctt atggcatatc aagcacttta tcgagtctgg
     4681 cgttcgcagc gtttcgaaga catcgttgga caaaaagctg tgacacagac actaaaaaat
     4741 gctattgttt cccacaaaac ctcacatgcc tacttgttta caggacccag aggaaccgga
     4801 aagacgagtg cagcaaagat tttcgcaaaa gcaatcaact gtccgaacag taaagacggt
     4861 gagccatgca acgaatgcga aatgtgtcga tcaattactg ctggtacgca agaagatgtg
     4921 atcgaaatcg atgctgccag caataacgga gtcgaagaaa tccgatttat ccgtgatcgg
     4981 gcaaattatg cgcctacaaa ggcagcgtac aaagtgtaca ttattgatga ggtacatatg
     5041 ctttcaacag gagctttcaa tgccttgttg aaaacattgg aagagccaaa agaaagtgtc
     5101 atattcatct tagcaacgac cgaacctcac aaaataccag ctacgatcat ttcacggact
     5161 cagcgatttg attttaaacg gatcaatacg gcagatattg tcgaacatct tgaatttatc
     5221 ttgaaagaat cgactatcag ctatgaacca gctgcattga atgtgattgc ccgttctgca
     5281 gaaggcggga tgcgggatgc attgagtatt cttgatcagg caatctcgtt tagtgaagga
     5341 acaattactt tagatgatgc catgcaagtg acaggtagct taactgacga aatgatggac
     5401 agttacttga gtgcttgcgt gaaaaaagaa gtaccagaag cattggaaac attgaaccaa
     5461 attctcgcag ccggcaaaga atcacgtcgc tttctagagg atatgttaca atactgccgt
     5521 gacttgctga tgtatcagca agcacctaac ctagttgagg aacaatcgag tacgatcaga
     5581 agtacattta cagaattggc acaaactgtt tcagcggatc agctgttttc aatgataaag
     5641 attttgagcg atactcaaaa tgaaattcgt tttaccaata gtgccgctat ttatttggaa
     5701 gtcgctacag tcaaacttgc acagctaccg tctgcttctg ttccttcagc agatgtagca
     5761 tttgcagatg atcagatagt aaaacagcta caacaagaga ttaaggaact aagaaacgaa
     5821 atcaaggaac tacgcagtaa tacaggaatt gagaatacaa aagtacagcc ggtacagcaa
     5881 acaaatcata aaaaacagac aagtacctat cgtgtaccga cggaacgagt atataaagtt
     5941 ctgcgggaag cgacaagaca gcatctgaac caagtaaaag aagtatggga agacttgctg
     6001 atgagtctat ctgtcactca gcgagcaatg cttaaagcaa gcgaaccagt tgcagctgga
     6061 cctacaggac tagtgattgc gtttgattat gagattgttt gtcaaagagc agcaaatgac
     6121 gaagaattgc agctttcggt acacaacaac ttaagccgaa tgatcaaaga ttatgcgcct
     6181 gatcctgttt atattacaag agaaagctgg ccagaacttc gaagaaatta tttgacgcag
     6241 gcacaagaag agggaacaga aaactctttt gcttctgaag actcagaagg agaaatcgag
     6301 ttgcttcctc aagaacaaga agtaccagaa gaaacacttg tagtaaaagc agaggaactg
     6361 tttggctccg aagctgttac agtggtagat gattaataaa aaggagtaga taattatgat
     6421 gcgtggaatg ggcaatatgc aaggcatgat gaaacaagta caaaaaatgc aaaaagaaat
     6481 ggcagaagca caagagctgc taaataaaac agagtttaca ggtaaagcca ccaatgaatt
     6541 ggtcaaagcg gtttttacag gcgatcgtcg catgaaagat atccaaatca atgaagaagt
     6601 cgttgatcct gatgatatcg agatgcttca ggatcttgtc gtaatggcag tcaatgatgc
     6661 gatcgcacaa attgatgtgg aaactgagaa aaaattaggg aaatacgcaa aaggtttgcc
     6721 aggattttag ttttaaactg aaggagtaat gaatatgcaa tatccagaac caattgcgaa
     6781 actgatcgac agctacatga aacttccagg aatcggtcag aaaacagcga caagactcgc
     6841 cttttatacg atcgatatga aagatgaagt agtaaatgaa tttgctaaat ctttgttaag
     6901 tgtaaaacgc gatttgcatt tttgtagtat ctgcggaaac atcacagaag aagatccctg
     6961 cgaaatatgt agaaatcctt cacgtgatcg aagcgttatc ctagtggtcg aagagccgaa
     7021 agatgtaatg gcaatggaaa aaatgcgaga atatcacgga ctttatcatg ttctccatgg
     7081 tgtgttatca ccgatggaag ggacaggtcc agaagacatc aatatttctt ctctgttgca
     7141 gcggctgcat gatgcagatg taaaagaagt gattatcgca acaaatgcaa caacagaggg
     7201 agaagcaaca gctatgtatc tctcacgtct gatcaaaccc gcaggtatca aagtgactcg
     7261 tctagcacat ggtctgtctg taggctctga tatcgagtat gcagatgaag ttactttatt
     7321 gaaagctgtc gaaggaagac gtgagttata aatttctaat taagtgtttt ttcagataca
     7381 atataaaagc agcacaagag atgggggcaa caatgtgaac ggactattta ttacaatcga
     7441 ggggcctgat ggtgcaggaa aaaccagcat cttgaatgaa ctttaccctc ttttgaaaga
     7501 agtagcaaaa acagaaatca ttcaaacaag agaaccagga ggcattccga ttgctgaaga
     7561 gatccgagca gtgatccttg atccaaaaaa tgatcggatg gatgaacgta cggaagcact
     7621 gctttatgca gcagcaagaa gacaacatct agtcgaaaag gttctgcctg ctctggcaaa
     7681 agggaaaatc gttttatgcg atagatttgt cgacagttct cttgcttatc aaggtgcagg
     7741 ccgacgcatc ggcgttaccg agattgctcg gttgaatgag tttgctacag aaggtactac
     7801 gccggatttt accctttatc ttgacgtaga ttcagacacc ggattaagaa gaatcaagaa
     7861 aaacagacaa aatcaaatcg accgtttaga ttcggaaggt ttggaattcc accagcgtgt
     7921 acgtcatgct tacttaaagc tggcagaaga gaatccggaa agaatccaca aagttgatgc
     7981 aagaaaaagc tttgaagaag tgctgcaaac aagttatcat acaattattg aacagtatcc
     8041 gcaatttttt gaaaactagg aaggtgaacg tttatgaaaa ttattttagc gattatccaa
     8101 gacaaagaca gcaaccgttt atcaaatgaa ttgattgatg caaatatccg tgccacaaaa
     8161 ctatcatcga caggcggctt ccttaaggct ggaaacagta cgtttatcgt gggtattgaa
     8221 gatgaccgtg tagaagaagc attggaaatc atcaaaaaaa cgtgtgaatc aagaaagcaa
     8281 tttgtatcta cacctgttac actggatatt tcgatggatg ggggcgtccc ttatccagta
     8341 gaagtagaag taggcggtgc cacagtcttc gtcttacctg ttgaaggatt ccatcaattt
     8401 taggaggagc tgagctatga atcaagaaat aaacttggct caaatgcaac cgatcgtgta
     8461 tcagcagctt caacggagtt ttgagcatgg gcgtatcgct catgcttatc tttttgaagg
     8521 ggagaaagga acaggaaaac atgaagtagg tatatggctt gcacagcacc tgttctgtac
     8581 ccaaatgaaa gacaatctgc cttgtggaaa atgcaacaat tgtcagcgga tcgaaaagaa
     8641 agaacatcca gatgttttgg ttattgaacc ggaaggacaa accatcaaag tcgatcagat
     8701 cagacgtttg cagacggaat tcagtcgcag cggttacgaa tcaagaaaaa aagttttttt
     8761 gatcaaagaa gctgaaaaaa tgaatagcag tgcggcaaac agtttgttga aattcttgga
     8821 agagccccct ggagattttt tagcggtgtt agaaacagat gcagtaggaa gaattctccc
     8881 tactattcaa tctcgttgtc aagttctgca ttttcaagaa ctttcgaaag aagcactgat
     8941 ccatcgattg caagaagagc agattccatt ggaaaaagca aaacttttgg ctttcttaac
     9001 aaacagttta ggaaaagcag ttgaaatctc tcaagatgaa tggtttaatg atgctaagga
     9061 cttgatttat caatggttcg tttacttgca aaagcaagat actcaagcct ttatctatgt
     9121 ccaaaaaaaa ttggtaaaat catttaaaga aaaaacacaa cagtttacag gattatctgt
     9181 cttgatgttt tattatcagg aagcacagcg tcaagcagta gcaaacggcg aatatggcaa
     9241 agtgaaacag ataaaccaga taattgaacg gatattgatc gcagagcaga aactacgcag
     9301 caatgtcagt tttcaggctg ttgcggaaca gttcgttttg catacgattt tttagcttgc
     9361 cgcaaaggat gatgatcaag gaggatggaa atggtagaag tagtaggtgt ccgatataga
     9421 gatgcaggtc atatctatta ttttgcacca ggagactctg aatatgttta tagtgacaag
     9481 gtattagtcg aatctcagca atccttacat attgctactg ttgtccttcc taaaaaagag
     9541 attgatccca atgaccttcc tgaagctatc cgtccgatct tgcataaagc gtcacaagct
     9601 gaattagaaa aagacaaaaa aaacaaaaaa gatgcttatg atgcattgaa aatcgcaaaa
     9661 gaaaaagtgc agttgcgtga gttggaaatg aaaatggtca aagccgaata cacatttgat
     9721 cgaaataaat tgattttttc tttcacagcg gacgggcgaa ttgactttag agaattggta
     9781 aaagatctgg ccgctgtatt tcgtactcgt attgagcttc gacaaattgg tgtccgcgat
     9841 gaagcaaaga taataggcgg aattggtcca tgtgggcgtc cattatgctg ttcttctttt
     9901 ttaggcgatt tcgtgcctgt atcaatcaaa atggcaaaag atcaaagttt atctttgaat
     9961 ccaacaaaaa tttccggttt atgcggacgc ttgatgtgct gtctgcaata tgagagcgaa
    10021 gaatacgaga cagcgaaaaa agaactccct gactttggaa aagtaattgc tacacctgat
    10081 ggtaaaggga aagtcgtagg gttgaatctg cttagccgta tcgttagtat tcggctgact
    10141 ggacgggaaa atcctgtcga atatgaatgg gatgaattga aagaatttat ccaagtaggt
    10201 gagatgaatg gataagagat cattatacga cggattgaat caattagaat cagagttgag
    10261 aaatacatta acagaacttg ttgaaatgaa gcatgctttg catgagatcg tagaaaaaaa
    10321 tacaactttg gagatggaaa atcaacgtct gcgggaacac ctgcgggaat tgaaccaagt
    10381 tgcacaagct ccagctgaaa atgtgaagca ggaactttct aagtctcgta tgaatcttga
    10441 aaaaatttat gaagaaggat tccatgtctg ctacgattta tatggatcaa gaagggacaa
    10501 tgacgagcct tgtgcgtttt gtctagaagt gatctatcgc gaaagcggtc gatgaccaat
    10561 aacaagagga gactggagcg gagaaatact gctccagtct ttttattaag gagattgtga
    10621 cagatgtata atcaaaaaag tttcaaggga aatccggtat atggggattt atatcttgta
    10681 ccgacgccaa ttggaaattt agaagatatg agctacagaa gtgttcgtat gctgcaagaa
    10741 tccgatctta tcgcaagtga ggatacgcgt aatacacaaa agttattgaa tcattttgaa
    10801 atccatacac cccaaaaaag tttacatgaa cataattata aagaacgtgt ccctcaattg
    10861 attgctttgt taaaagaagg aaaaacgatt gctcaagtca gtgacgcagg aatgccttcc
    10921 atcagcgatc ctggacatga gctagtttta gcctgcataa aagaagggat ttctgtgatc
    10981 tctatcccag gacctacagc tgggatgacg gctttgattg cttcagggct agtgcctcaa
    11041 ccatttttat tttatggctt ccttccacgt aagaaaaaag aacagaaaga aacattgaaa
    11101 aagctaaaag aacagacagt tacgctgatt ttttatgagt caccttatcg gatcactgct
    11161 acattgacga attttttaga aatttttgga aatcgccaag tcgttttgtg tcgggaattg
    11221 acaaaggtcc atgaagaata ccttagagga agtacggaag agctactcgc ttatatcgaa
    11281 gaacatccag taaagggtga atgttgtctg cttgtagaag ggaatttgtt cgaaatgcca
    11341 gtgacggaag ttgatgaaga gttgggaacg ttaaaggaac aagtagaagc aaagatcgct
    11401 tcaggcgaaa aaccaaatgc tgctatcaaa gcagtagcgc tgaaaaatgg gctgaaaaaa
    11461 caagaagtct accgtcagta ccatgaatta gactgagata gtataagttg tctataatga
    11521 aagactaatg aggaaatcag ctactaactt ttcaaatctc ttgaacgcga acgaatgttc
    11581 ttgtataatg aataagaggt gaggatgatg gagctgcaat ttcctttaca taatgataca
    11641 tcaagaaaaa tcattcatat tgatatggat gcttttttcg cttcggttga agaacgagat
    11701 catcctgagt ttcgcggaca tccattagta attgccagac accccagtga cactggtggg
    11761 cgtggagtag tgactacagc aaattatgaa gcaagaaaat tcggaattca ttcagcaatg
    11821 agtgcacaaa aagcgtatga gctttgtccg caagccatct ttaagcctgg cgaccatcat
    11881 aaatacagcg atatttccaa acaagtacgg gaagtattct atcgttatac agatattgtg
    11941 gaaccagtta gtatcgatga agcgtatctt gatgtgaccg aaaataaaat caattgcaag
    12001 tcagccatca aaatcgcccg aatgatccaa gcagatattt gggagagtgt ccagctaaca
    12061 tgttctgcgg gagtaagtta caataagttt ttggccaaat tagcatcaga ctatcaaaaa
    12121 cctagaggaa tcacggtagt tgcccctcaa gacgctgtgc catttttaaa agctttgcca
    12181 attgaaaaat ttcatggggt agggaagaaa actgtaccta gaatgcatga gctagggatt
    12241 tatacaggaa aagacctata tgaatgcaca gaaatgatgt tgattcgaaa ttttggaaaa
    12301 atgggctatt ctctttatcg taaagtcaga ggaatccatg attctccagt aaacgttaca
    12361 agagagcgaa aatctgtggg aaaagagcat acttatgggc agccgttgca aacggaagaa
    12421 gcagtgctga ctcagttgcg ccaactagct gaaaaagtag aagaagcact gagaagagtc
    12481 caaaaacatg ggaaaacagt agttttgaag gtcaggtaca cggactattc tactgtgacc
    12541 aaaagagtga cattgccaga atatatctat aaaaaagaag cactctttta tcaagcaagc
    12601 cttatatggg aggaaatctt gggagtagaa aaaggaatcc gattgttagg gattactttg
    12661 acgaatctgg acccgatgac ttatgaaaac atcgtccttc cattatggga aaatcaagaa
    12721 atataaaaac agctttgaga gttgaaaccg gaaatccgtt ttctctccca aagctgtttt
    12781 tatatcaagt cttgcttttt gatttgttca taggtttctg ttgcatccgt acatttttcc
    12841 caaatcacaa cttgattgga ttctaaagat ttttggacgt caatgatccg ctggttgcta
    12901 cttccgcgaa attgtagttt taggtcacgt ttgcttaatt caaagcgtcc gtcaacaagg
    12961 atatcgattt gcgaaaggag ttctaattta tcttctgaat cttgtagcaa ttcatcaaag
    13021 gcatagcctg tccatgacca gatatctttt gtcgtaccga actcttttcg gatacgtcgt
    13081 acgacttgca gacaaacatc tgtattcaaa aaaggttcgc caccaagtaa agttaatcct
    13141 tgcacgtaat cgttgcgcaa gtcagcgatg atttgttctt ctaattcagg tgtgaatggt
    13201 ttgccatatc ggaaattttg tgcagctttg ttgaagcagc cttcgcaggc aaataagcag
    13261 ccactaacgt aaagactgtt gcggacacct tcaccatcga caaaattaaa cggtttgtag
    13321 tcagcaatat agttttggct gtagtctttt gctaaccatt cttttggttt tggatttctc
    13381 ataaaaaaac ttctttcatt taaggttcaa agatatagct attgaagata aaatctactc
    13441 gaccacataa ataagaacaa actgacctaa atccattgtt caagaaagct tgttcttatt
    13501 ttgatagagg gcgttcaaag atacaaagcc atcgaaagag ggtggtgaaa aaagacttgt
    13561 catagcctct tattctgaat aaacggtgtt caaaagcttg catctgcagt aataagccca
    13621 actaaacgaa aaatatgagg aactattttt gtttagctat tcttattact tgatgcagga
    13681 cagctttttc acaacctcat ttcatgtgtt tcacacgtga ggaaatttct ttatggcggc
    13741 catgtaccat cggacgagct tgcggatttc ctaaatagcc acaagtacgt ttgactacat
    13801 cacatgtttt tggatcatga tttccacatt ctggacattc gaaccctcgt tctgtcggtt
    13861 tgaaatcacc ttcgaaacca cattgatagc aatgatcgat tggcgtattc gtaccaagat
    13921 acccgatttt gtcatatgca aaatcccata cagcttctaa agctttcgga ttttgttgga
    13981 gcatagggta ttcacagtag tggatgaatc cacctgaaca atattttgga taatccttct
    14041 caaattccaa tttttcaaat ggcgttgggt ttttacgcac atcatagtgg aaactgtttg
    14101 tataatattc tttatctgtg atattttcga tgatgccgaa tttttctgta tccaaacggc
    14161 agaaacggtc agttagactt tcacttggag ttgaatagac actgaaatga taaccatatt
    14221 catcccccca tgcatctgca ttagctttta attctttgac gatatcaagt gtgaaagctt
    14281 ttgcttctgg gtttgtttcc cattcgccac cgaagaaagc tgaagcaact tcatacaatc
    14341 caatgtagcc taacgataca gtagctcgac ggtttttgaa taattcattg acagaatcgg
    14401 tacgtgaaag acgtttcccg aaagaaccat acatatataa aattggcgca tttgctggaa
    14461 tggcttcttt acaacgttct acacgataga gaagcgcatc tttcatgatt tctaagcggt
    14521 cagacagtaa ctgccagaat ttattctgat ctccttttga ttcgatcgcg attcttggca
    14581 aattcaatgt aacaacacct aaattcattc gtccgacatt tacttcttga ccattttcgt
    14641 ctttccaccc ttgcaagaat gaacgacagc ccataggtac tttgaaactt ccagttaatt
    14701 caacgatttt atcgtaattc agaatatcag gatacattct tttcgttgca cattccaatg
    14761 ctagttgttt gatgtcgtaa ttaggatctt cagggtttaa gttcacacct tttttcaaag
    14821 tgaagatcag tttagggaag atagctgtcc gcttttcact ccctaatcca ttgattcgaa
    14881 tctgtaaaat tgctttttga atttctcttt caaaccagtt ttctcctagt ccaaatccta
    14941 atgaagtgaa aggagtttgt ccatttgaag taaataaggt attgatttca tattccaaac
    15001 tttgcatagc atcgaagata tcttttttgg tttttgcttt tgcatattct ttttggcgtt
    15061 ctggcgaatc gatccattct tccgcgtctt ttagatgttt tttatagttc aattctgcaa
    15121 aaggagcaag caattcatct gttcggtcgg cagaacagcc accatattga cttgacgcta
    15181 cattggcaat aatctgtgag atttgtgctg tggcggtctg gatagatttt ggcgattcga
    15241 cttcggcatt gccgattttg aacccattgt tcagcatgcc tttgaaatca atcaagcagc
    15301 aattcgtcat cggtgtataa ggatgataat ccaaatcatg gtaatggata tcgccttttt
    15361 gatgcgcatt tgctatatgt ggtggcaaca ttttcagtcc gattgatttt ccaacgatcc
    15421 cggctgtcag atctcgctgt gtattgaaaa cattactgtc tttatttgcg ttttcgttca
    15481 cgacagtttg gtccttgttg attagctttt caatcgaaaa gttgatatct gttgctttac
    15541 tgcgagcaaa atctcgtttg gtacgataat tgatgtactc ttctgctaat tcatattcgt
    15601 ttttttctag taatatatgt tccacgatgt tttgaatctc atagattttg acattttcag
    15661 caaaccggct tttgatttct tggacaacca agtctgcaat catttgaatt ttttcataag
    15721 taagtggtga taatgagccg tgaattttct tttctgcttt gatcagtgct tcattgatct
    15781 tttgttcatc gaaatcaaca tggcggccat cgcgtttgat gactttcatc tgggatgtct
    15841 ctggctgctg agtaacgaca gttttttctt cataatttac tttcattatc ttcaactcct
    15901 gctaaattgt tcttgtctat cataaaacaa aatcggggaa ggagcaacta aatatcggct
    15961 atatatagta ttcaatgtgc tgtataattg gatttctata ctatatgtag tgtatatttt
    16021 attgtgaaaa aaggtgaaaa cttgtagctg gtggatttgt tttttgcata tttaattttg
    16081 ctgaataaaa taattgaata aaatttttta ttcagtccaa gtaaaagtga caaaaacata
    16141 agagcaaatt attcgtagat atgctactat gcaaataaat agaaatgaaa aatgaaagag
    16201 gaggagaaga attggaaaag atgatcgaag tgaaacatct aagtaaaagc tacggcagca
    16261 aactttctcc cgtcaaagcc ttagataata tctcattcac agtagaagaa ggcgagtttg
    16321 ttgggattat gggcccaagt ggtgcaggaa agacaacgct gatgaatatc ttgtccacga
    16381 tcaatttgcc tacgatgggc agtgtctcga tccaaggaaa agaaatcaca aaaatgaaaa
    16441 acagtgagct cagtgacttt cgtcgaaaaa aactaggatt catttttcaa gagttcaatt
    16501 tgatcgatac cctaaatgca aaagacaata tcctattgcc cctagcagtt gaacggatga
    16561 ctaaagagga aatggaaaag agagtaagac atgtagctca attgttgaat atagaagaac
    16621 tgctcaaacg gtatcccgat gaactttctg tcggacagag acaacgaatt gcagctgcta
    16681 gagcacttgt tgttcagcca caagtgattt ttgcagatga accaactggc tctttggact
    16741 cgaagtcggc tacagaattg ttgaactact tgaagaccat gaatcaaaaa gaaaaagcaa
    16801 cgatactgtt agtcacccat gatccatata cagcaagcta ctgcgataga attttattta
    16861 ttaaagatgg tgtgattttt tctgaagttg tcagaagagg gacaagaaga gagttctttg
    16921 agaaagtaat agacatgcag gcgacgattg gcggaggagg aaaaatgaat gctgtataaa
    16981 ctagccgtta aaagtttttt tcggcagtca cgaggctatc ttgtttattt tttcagtttg
    17041 actttatccg taatgatcta ttattccttc agcgcaatga cctacgatca atctttagtc
    17101 agaagagcaa gtcaagatac ttcgatagat ggtggattga gttttggtgg atttatcatt
    17161 actgttgttt tgcttttttt cgtattcagt gccaaccgat ttttcttgaa tagaaggcaa
    17221 aaggaaatag gcatctatca gctttttggt atgaataaac tgcagatatc aggtatatat
    17281 gtgttagaaa tcatgataat tggattgttc gcctgtattt ctgggatatt actcggtatt
    17341 attttttcca aactcttttc aatgattttg gttcgaatga tggatatgga tcttactagt
    17401 ccatttttta tctccattcc atcagtagta gatactttat ttgctttttt catcatcctt
    17461 ttagcagtat ctgtatattc aatatggaaa atatggcgct atccaatgat tcgaagtttt
    17521 ggagaaaaag aacaagcaga tagttcaaca atgcgcttca ggacaaggca ccgattactc
    17581 gcagtcatcg ggttactact tttaacgagc ggatattttg gagctagtca ttttagggaa
    17641 atcacttcat ggatgatcag ttatcattat cttgatacat tagttttctt ttttcctttt
    17701 ttaatttttt tcgtttgtgt ggtcggtact tatttgtttt tctgttatac atttcggtta
    17761 tttttgatca tattgagtaa atggaagatt tattactatg gtatcaattt tttgataatg
    17821 ggtaacacgc aagttcattt gctcaaaagt tggcgtacaa attctctgat tacagtcatt
    17881 ctcggaattt ctttaatgac aattggcggc acggctagta tctctactat tttatcctac
    17941 caggagaaag tttcctcccc gatggactat cagtttgatg ttgagacaga gaaaaaaatc
    18001 aagccgattc tagcagagga agggcagaag ataactaaag aaatcacctt gcattataaa
    18061 gtcattggga gtatctatag tcaaaatctc gaagaaccta tgtttcaaac tgctaatctg
    18121 atcacggaaa cacaatatca agccttcaga aaagttaatc cagatttgcc gaacatttct
    18181 ttgaaatcac ctggtcatac tgttttgctt gatcaaatgc aaactctttt tcggaatatt
    18241 tcattatatg gatctagtat tggactgcca gataatcaag aattgaagat tcaacaaatg
    18301 cttccaagtg tattaggtga cgaaacaatg gtatatctaa gtcctgtact gattgtagat
    18361 gataccgttt ttgaaaaagc aaaaggtgtg gattatcaag tagtcaatgt agatgtatca
    18421 ggtggaaata atgaaaaaat agatgaacga ctgagcaatg acctgtcgat aaagtggctc
    18481 aatgctgttt attattctta tgatatcgta aatggaagtc tacaaggaga aattagcaca
    18541 aagaaacttc cggatgacca aatgaaggaa gcacaattta gccaagaaag ctctcgtttg
    18601 aatttctctt ccagctattc gaaattgcgt gcagttaatc ggcgaatcgg tatcaatatt
    18661 tttgtcacac tatttgtcgg gatgatcgtg attattgcta ctggtagtat tttgacagtc
    18721 cggcaattag cagaagcaga ggaagaaaaa gaaaattatc acctattgac taagctgggg
    18781 attccacaaa gaaggatcag acggatgatc ttcgagcaaa acgcacttac cttctttcca
    18841 cccatgatat tagggatcac tcacgcagta tttgcaatca atgtcttcac tcagtatata
    18901 aaaactgccg attactggtt ggcttatctt gtgagtggct tattgatcct tatctatctt
    18961 ttgctttatg tcattacgag tcagttatat tgtcggatta tcgaggaatg atatacggaa
    19021 taagacaaga taataaatag aacta
//
LOCUS       DAZKBH010000047        18740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000047 DAZKBH010000000
VERSION     DAZKBH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.047"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..531
                     /locus_tag="ACG8OO_001546"
     CDS             256..531
                     /locus_tag="ACG8OO_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045431.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="ACG8OO_001547"
     CDS             544..2616
                     /locus_tag="ACG8OO_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9045432.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2784..4667
                     /locus_tag="ACG8OO_001548"
     CDS             2784..4667
                     /locus_tag="ACG8OO_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9045433.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4679..5626
                     /locus_tag="ACG8OO_001549"
     CDS             4679..5626
                     /locus_tag="ACG8OO_001549"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9045434.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="ACG8OO_001550"
     CDS             5646..6173
                     /locus_tag="ACG8OO_001550"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9045435.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="ACG8OO_001551"
     CDS             complement(6236..6889)
                     /locus_tag="ACG8OO_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9045436.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="ACG8OO_001552"
     CDS             7021..7863
                     /locus_tag="ACG8OO_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9045437.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="ACG8OO_001553"
     CDS             8021..8917
                     /locus_tag="ACG8OO_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9045438.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="ACG8OO_001554"
     CDS             8920..9633
                     /locus_tag="ACG8OO_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFP9045439.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="ACG8OO_001555"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="ACG8OO_001555"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9045440.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="ACG8OO_001556"
     CDS             10168..10395
                     /locus_tag="ACG8OO_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFP9045441.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="ACG8OO_001557"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="ACG8OO_001557"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9045442.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="ACG8OO_001558"
     CDS             complement(11181..11723)
                     /locus_tag="ACG8OO_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9045443.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="ACG8OO_001559"
     CDS             complement(11960..12358)
                     /locus_tag="ACG8OO_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9045444.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="ACG8OO_001560"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="ACG8OO_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFP9045445.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="ACG8OO_001561"
     CDS             13471..14238
                     /locus_tag="ACG8OO_001561"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9045446.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="ACG8OO_001562"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="ACG8OO_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP9045447.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="ACG8OO_001563"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="ACG8OO_001563"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9045448.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="ACG8OO_001564"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="ACG8OO_001564"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFP9045449.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DAZKBH010000048        18501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000048 DAZKBH010000000
VERSION     DAZKBH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.048"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..1247)
                     /gene="hflX"
                     /locus_tag="ACG8OO_001565"
     CDS             complement(18..1247)
                     /gene="hflX"
                     /locus_tag="ACG8OO_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFP9045450.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1249..2166)
                     /gene="miaA"
                     /locus_tag="ACG8OO_001566"
     CDS             complement(1249..2166)
                     /gene="miaA"
                     /locus_tag="ACG8OO_001566"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9045451.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2170..2907)
                     /locus_tag="ACG8OO_001567"
     CDS             complement(2170..2907)
                     /locus_tag="ACG8OO_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9045452.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2998..3525)
                     /locus_tag="ACG8OO_001568"
     CDS             complement(2998..3525)
                     /locus_tag="ACG8OO_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045453.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3705..4298
                     /gene="clpP"
                     /locus_tag="ACG8OO_001569"
     CDS             3705..4298
                     /gene="clpP"
                     /locus_tag="ACG8OO_001569"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9045454.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4402..4887)
                     /locus_tag="ACG8OO_001570"
     CDS             complement(4402..4887)
                     /locus_tag="ACG8OO_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9045455.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5040..5237)
                     /locus_tag="ACG8OO_001571"
     CDS             complement(5040..5237)
                     /locus_tag="ACG8OO_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045456.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5332..7092)
                     /gene="efrB"
                     /locus_tag="ACG8OO_001572"
     CDS             complement(5332..7092)
                     /gene="efrB"
                     /locus_tag="ACG8OO_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFP9045457.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7089..8819)
                     /gene="efrA"
                     /locus_tag="ACG8OO_001573"
     CDS             complement(7089..8819)
                     /gene="efrA"
                     /locus_tag="ACG8OO_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFP9045458.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9212..9424
                     /locus_tag="ACG8OO_001574"
     CDS             9212..9424
                     /locus_tag="ACG8OO_001574"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFP9045459.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9426..11105
                     /locus_tag="ACG8OO_001575"
     CDS             9426..11105
                     /locus_tag="ACG8OO_001575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9045460.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11359..11559)
                     /locus_tag="ACG8OO_001576"
     CDS             complement(11359..11559)
                     /locus_tag="ACG8OO_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9045461.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11709..11852)
                     /locus_tag="ACG8OO_001577"
                     /pseudo
     CDS             complement(11709..11852)
                     /locus_tag="ACG8OO_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            complement(12087..12749)
                     /locus_tag="ACG8OO_001578"
     CDS             complement(12087..12749)
                     /locus_tag="ACG8OO_001578"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9045462.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13141..13200
                     /locus_tag="ACG8OO_001579"
     CDS             13141..13200
                     /locus_tag="ACG8OO_001579"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFP9045463.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            13258..13989
                     /gene="erm(A)"
                     /locus_tag="ACG8OO_001580"
     CDS             13258..13989
                     /gene="erm(A)"
                     /locus_tag="ACG8OO_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFP9045464.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(14115..14897)
                     /locus_tag="ACG8OO_001581"
     CDS             complement(14115..14897)
                     /locus_tag="ACG8OO_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFP9045465.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(15051..15428)
                     /locus_tag="ACG8OO_001582"
     CDS             complement(15051..15428)
                     /locus_tag="ACG8OO_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFP9045466.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            complement(15435..17327)
                     /locus_tag="ACG8OO_001583"
     CDS             complement(15435..17327)
                     /locus_tag="ACG8OO_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9045467.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            complement(17324..18409)
                     /locus_tag="ACG8OO_001584"
     CDS             complement(17324..18409)
                     /locus_tag="ACG8OO_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9045468.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
BASE COUNT         5515 a         3834 c         2966 g         6186 t
ORIGIN      
        1 gttaaaaaaa taaaatttta ttggtttttt cttaaccatt ttgaattggt ttttgcaaac
       61 cctttaactt catattcgtt tgtttcttcc atgtacgctt cgtccaaaac cattgtttgt
      121 cttctcaact cgcttaattt ctgtccttcg ttgcttggca cagaaagtgt ataaggggtc
      181 attagttcca tcagctgacg tttgattgct tcgatcaacg ccatcttacc ttctttcgtc
      241 tgagtagaaa tcaatacatt tgggaatagc gtaggcgtga acatcgctgg gtcgatctgg
      301 tctgccttat tgtaaacagt cagtatcggc atttctttca tattcagttc atccatcaat
      361 tttaagacag tctgctcctg taaaatacga tctggagaac tagcatcgac tacatgtagc
      421 aaaagatcca tattttgact ttcttctaat gtagaatgga aagcatcgat cagttgcgta
      481 ggcaggtctt ggatgaaccc gacggtatcc gttacagtaa tctcaaatcc ttcagcaaaa
      541 cgccatctct tagtcaatgg atctaacgtt gcaaacaatt gatctttcga ataagtatcc
      601 gcttgtgtca atagattcaa gatagttgac ttcccggcat tcgtgtagcc aataagtcca
      661 atctgaaaaa tctcgctatt ttttcttttt tggcgattgc gctctcgatg agcttctact
      721 tctttcaatt ctttcttaac agcaaaaatt ttattccgta tatgtcggcg gtctgtttct
      781 aactttgttt cacctggccc tcttgttccg atcccaccgc caagacgaga taatgttttc
      841 ccttgcccta ctaaacgagg cagcaggtat tctaactgag caagttcaac ctgcagcttc
      901 ccttctttcg aacgagcacg catcgcaaaa atatctaaga taagctgcac gcgatcaatt
      961 accggaagac caacagcatc ggtaatcagt tggctctgtc tcggagttag ctcatgatta
     1021 aagatgatca aatccgcttc atgcgcatcc gcttgttgaa ctaattcttg taatttccct
     1081 ttaccgataa tcgtttgccg atccacttgt ggtctctttt gtgtaattgt gaatacgact
     1141 tctcctgatg cggtattcgt cagctgaact agctcttcca ttgactctga aaagtaacgc
     1201 tggtttgctt cggtttctac ccctacgatg atcacttttt cttccatttt attccgcctc
     1261 cagccatttt ttgattgttt gctcaatctg acttttttga tttggctcac ttataagatc
     1321 aaaccactgt gcatccaaac ggttacgaaa ccacgtcaac tgacgtttcg cgtatcttct
     1381 cgaatgtagt ttgacttgtt ctgttgcttc ttccaatgtt tctttccctt caaaataagg
     1441 gaaaaattct ttatatccaa taccttgact ggcctggact tctccaagct catacacctt
     1501 acgcgcttct gctactaaac cttctgctag catcaaatcg actctttgat tgattcgttc
     1561 ataaagaata ctacgcttcg tatctaatcc aatcatgagg taatcataca attgctttgg
     1621 ctcttcttta ggtgccgtga tactatgtcc tgtcgtctct aaaacttcca gagctcttat
     1681 cactttgcgt tgattgttcc agtggatttt ctcagcagct gccgggtctt tttctttcaa
     1741 ctgattccat aatgcttggg aaccaattgt ttcagctaac gtttcatact tttttctaat
     1801 ctcaggattc gcttcttctg tcgctcctaa ctgataatca tatagaaggg attggatata
     1861 gagacccgta ccaccagcta tgataggtag gtgccctcgg ttcgcaatct cggtaatcaa
     1921 ttgtcgacct gaatgctgaa aatcagcggc tgagtaacgt tgagtaggtt cgatcacatc
     1981 aatcaaatga tgcggaacat tattcatctc ttcttctttg atttttgctg taccgatatc
     2041 cagattttta tagatttgca atgaatcacc actgatgatc tctcctccaa atagttgtgc
     2101 gagttccaca cttagcgctg ttttaccgac tgctgtcgga ccgacgatca ctaatacttt
     2161 ttccatattt tacctacttt tcgttgattg tttccaccga agagctttct ccggaaaatc
     2221 agttattatt ccatctattt tgagttcttg acatttggcc atttcttctt catcgttaat
     2281 cgtccatgca cgaattgctt ttggaaaatc agctgcctgg actggatgtt cgaacaacca
     2341 agtatttttt ggatgtactc cttctatata aggggtttct gctgctagtt ggatcttttt
     2401 atcagaagta ctcatcaata agtccagctg tatctccggt tccagtaatg cgattttttc
     2461 caaagaatcc aaatgaaagc tgcaatatgc atgttgaaaa ggccaatctc tactttttaa
     2521 tgtttcggct aatttttctt cgataccggg atattcatac tgatcagttt taatttcaat
     2581 cattagcatc cctcggtaat ttttttggag gagcagtcct aacacttctc tcaattcagg
     2641 aactttagct gctggaaatt ctttgctgaa tttgccccct gcattcaatt ttttgagttc
     2701 ttccaatgtt ttttctcgaa tcagcccttt accgttagtc gtccggtcca cttcttcgtc
     2761 gtgcataacg atcaaataac catctttgct tagatggaca tctagttcga taccatctgc
     2821 acctactcgt acagcttctg caaatgcagg aagcgtattt tctgggtggg tgccgctgct
     2881 tccgcgatgg gcatagattt gtaacatctt tcttcactcc gttcagttct attgtatcat
     2941 taagaaaaaa agaaagcatt accacgctct catgtgcaac ggcaatgctt caaaatatca
     3001 gctccaaaca tcatctttcg agaagaaacc aatcggctgg cgcgtcatag tcttcaactg
     3061 agcttttttt tcattgatca tttcagtcag actatccgta aaattctcta aaatcacgcg
     3121 tccgccttta taagcatatc caccaatctt cattgtttca tctggagata aaatcacttt
     3181 tcgatgggag atattcttaa atagctcgtc taaaatataa tttggcagat aaaaactatt
     3241 ttgcgctgaa aatgattgga gatacgcaaa atcgtcaatc gtgatataac aatcatcaaa
     3301 tgcatcaaag ctgacatatt cttccatcag cgttgtccgt ttttgcaaga tctcgttgat
     3361 ctttttattt aattgattca gcgtctcgat ttctttgact ttcaattcca attcacgtct
     3421 acgctgttct ttattttcca agtgacgcat ttgcagtatc atgccgatat agacaactac
     3481 agcacctgtc agcactccga taaggccagt aataccaact atcaatctgt tgttcctcct
     3541 tgttttgttt ctctgcttat cataccataa agcgggttta tgcgactaat ttgcctaagt
     3601 tttttatttg accttttttg accaaaaatg tatactacat tttaggtatt agaaactaaa
     3661 ttatggtaca ctatttctaa acgaaatcaa ggaggaactc tattatgaat ttaattccta
     3721 cagtcattga acaatcttca cgaggtgaaa gagcttacga tatctactca cgtttattga
     3781 aagaccgtat catcatgtta agcggccaag taacagatga tcttgctaat tcgatcatcg
     3841 cccaattgct tttcttagac gctcaagatt ctgaaaaaga tatctatctt tatatcaatt
     3901 ctcctggtgg ttcagttaca gcaggtatgg ctatttacga cacaatgaac tttgtcaaag
     3961 cagatgtcca aacaattgtt atgggtatgg ctgcttcaat gggaagcttc ttgctgacag
     4021 ctggtacaaa aggtaaacga tttgctttac caaatgctga gatcatgatc catcaacctc
     4081 ttggcggcgc tcaagggcaa gcaactgaaa tcgaaattgc ggcacgccat attttgcaaa
     4141 cacgcgaacg cttaaacaaa attttagctg aacgcactgg acaaccgctt gaagtgattg
     4201 aaaaagatac agatcgtgac aactatatga cagctgaaca agcaaaagca tatggattga
     4261 tcgatgaagt catggaaaac agttcttcat taaattaatc tgtttcaaag gaagcttttt
     4321 taagctctct tatctaaacc aaataaaaaa ggatggtcaa ctcaaactga ccatcctttt
     4381 ttattttatg gatgctactg cctatctttg ctccttgaat tttccttgcg catgcatccg
     4441 acctatctcg acataatgct ggatgttttc tttgtttttt tgtatttctt cttttgtcaa
     4501 aggacggatc actttagctg ggcgaccaaa agccaagaca ttaggaggaa taacttttcc
     4561 ttctgttact aatgatccag caccaatcaa actattctca ccaataaccg catgattcaa
     4621 tatcgtggtc cccatcccga tcagcgcccc tttttcgatt gtacagccat gtagcatgca
     4681 ctgatgtcct accgtcacat tctccgcaat atctacaggt gcatcatgat cgacatggat
     4741 gattgtacca tcttggatat tcgttctttg tcctattttt atccaattac tgtccccgcg
     4801 taaaacagct tgataccaga tcgtcacatc ttctgacaat gtcacatcac caatcactgt
     4861 ggcatttgat gctataaacc gttccatatt tatttttctc ctttcatttt ttgtagaaac
     4921 taatcctatt gaataaaggt atcatagcat atatttattt cataagaaaa aaaacaggta
     4981 aaacctgctc aggccagatg tctatttaca gtatctggta actgacgttt tacctgtttt
     5041 tattgtacat ctatattttc ttctattact ttttttaaaa ttaattcgta cacttctatt
     5101 tgtgctgcca agcgttcgat ttgaaactca ttttcaaaaa aatcagctga atcaaaaggc
     5161 ttcgtctgac agacgtgcgt taaacgatca acttctcttt tggcttcagt aattttttca
     5221 tttaaaaact gttccatctc cgtgtcctca cttttcttga taactttact ttaccattat
     5281 caagaataga gaacaaaaaa taagttttaa actgttttat tcttatatga cttattctgc
     5341 ttcctcagca aattggctgt tatacagttt ttcataaaat ccaccttgtg ccaataaact
     5401 ctcatgcgtt cctttttcaa tgatctgacc ttgatccatc actagaatca gatctgcatc
     5461 acggattgtc gataaacggt gagcgatgac gaaacttgtc cgtccctcca tcacgcgatc
     5521 cattgctttt tgaatcaacg cttctaaacg ggtgtctact gaacttgttg cttcatctaa
     5581 aatcaagatt ttaggatcgg agaccactgc tcgggcaatc gtcagcaact gtttttgtcc
     5641 taaagacaca ttgtctccct cagaattgat ggccatatca tatccatctg gcatcgttcg
     5701 gataaaatga tctacgtttg ctgttttcgc agcatcaact acttcataat ctgtcgcatc
     5761 aagctttccg aagcggatat tatctgcaat cgttccttga taaagccatg catcttgcaa
     5821 gaccatacca aatagcgaac gcacatcact gcgactcatt gatttggtgt cgattccatc
     5881 aatccttatt gctcctttgt ctacgtcata aaaacgcatc aatagattga tcaatgtcgt
     5941 cttgccggca ccggtcggac cgaccacagc tacggtttga ccagctttca cttcaaaatt
     6001 caaatcttgg attaatggtt tcttcggatc atatgcaaaa tcgacatgat cgaatgtcac
     6061 atctcctaaa atcgtttctg gcagtgaagc atctactttg ttttcttttt cttctggctc
     6121 atctaaaatc tcgaatacac gtttcgttgc cgctgatgca ctttgcaaaa ctgaagacaa
     6181 ttgtgtcacg ttccccattg gctgactgat ttgccaaata tattggatga atgcttgtag
     6241 ttgaccgaca acgatcgccc cagtaataac ataaaagctt cctagaacag ccataccaat
     6301 ataagtagca taggcagtta attgaactag aggcatcatc aagccagaaa tgaatgctgc
     6361 acgaaaccca aagtatttca agctgtgatt aacttttttg aatccttcca atgtttcttt
     6421 ttcgcgtcca tatactttca atacactaaa acctgtcata ttttcttgga cgtagccgtt
     6481 tagctcacca agagaattct gcatgctttg aaaatatttc tgcgaagcat tgatcactcg
     6541 ttttgagatc cagatcgatg ttggaatcat gatcatagag atcagcgcca tcatcggctg
     6601 gatataaaac atcattgctg cagccaacgt aattcccaat atcgcattta cgataccaat
     6661 aaaagcttgt tgtaaagcac cgctgactgc atctacatca ttcgtcacac gagacaaaat
     6721 atttccttgc tggtttttat cgaaatatga aaccggaaga cgattgattt tttcttctat
     6781 gtctcttctc aaatcatgca tggcactttg aacgacattt gtcatcaaaa caccagaaag
     6841 taattgcgaa atacaatacc ctattccaac aactgtgatc caaatgagac atttgaatat
     6901 ataagaaaaa ttgattgatt cgccattggc aatattacgg ctgatttctg tagtaggaag
     6961 tcctgtaaca tatggcaaag ccacgttgaa agccactgtc agaactgtaa ataggatcac
     7021 taaaagaaaa gtctcacgat acggtctgat atatcgagct aaacgtttta aggaagaaaa
     7081 ggtttttttc atgctaattc ctcctttgat aattgagaag cggcaatatc ataataaatc
     7141 gggcattttt ccaacaactc ccgatgcgtc cccataccga cgacttctcc ttcatttaat
     7201 acgatgatct tatctgcatg catgatcgtt cccacacgtt gtgctacgat caatacagta
     7261 gattctgtcg tttctttttt cagacgagca cgtaacttcg catccgtttg ataatctaat
     7321 gcagaaaagc tgtcatcaaa aatatagatt tccggacggc gtatgatggc tctggctatt
     7381 gccaatcgtt gtttttgtcc acctgaaaaa ttggaaccac cttctgataa atgttcatta
     7441 aatccatctg gttttcgaga aatgaattct gaagcttgag caatctcagc tgcacgttcc
     7501 atttcttctt gcgtagcatc ttctttcccg tatcgcaaat tatctgcaat cgttccagta
     7561 aatagcagcg ctttttgcgg aataaaccca atcttatctc gcaacttgct tagccgataa
     7621 tctctcacgt ctactccatc gatcaagatc tttccttcac tcacatcata aaagcgaggg
     7681 atcaattgga tcaacgtaga ttttccgcta ccggtacttc caataaaagc aacggtctct
     7741 cctggagagg ctgtaaaact gacatttcgt atcactggct cttgcgaatg gcctggataa
     7801 gcaaatgtta cattttgaaa ttcgatatac cctttagttt ttgtatcaga tataccattt
     7861 ggatcttcta caatcgaagg tttggcatct agggcttctt ggatacgtcc agctgaaaca
     7921 gctgctcttg gatacatcat gaacacacta gcaaataaca tgaatgagaa caaggcatgg
     7981 aatatatact cgataaacgc aatcaaatct cctacacgaa gctcgcctgc atcgatttgc
     8041 actgccccgc tccagatgat caataccatt acgatattaa ataagaaata aaaacccggt
     8101 tgtgcaacag ccatcaaacg gaataagctt tttgaactag aagcataatt ctcattgact
     8161 acagcaaaac gtttttcttc aaacttttca ttgacgaatg cacgaatcac acgcaatcct
     8221 gaaagatttt cccgcaagat actgttgatt ttatctaagt ttttttgttg ctttttagat
     8281 aacggttcag acaccttagc aatcgctacg actgccaata atagtaaagg taatgcacct
     8341 actacataca aactgagtgt cacacttgta cgcatgacca tgtacaaact gacaatgaac
     8401 atcattggtg ccataaaacc gatacgtaaa atattttgca ggaacaacat gatctgataa
     8461 gcatcgtttg ttgtacgagt gatcaatgaa gatacgccta gattttcgta ttcctgatgc
     8521 gaataacttt ggatcttttc gaacaaatca tctcgaatat ctgctacgat attagtcgta
     8581 atcctcgaac caaaataacc taacaagata ttcaaaataa taccgattac agtaataacg
     8641 atcataaata tcccttgttg cttcacataa tcaaaatcat cattgcggat tcctacatcg
     8701 atcatacgtg ctaaaatcgt aggaagtcct aattcaatca aaataaaacc aaatacgcat
     8761 atgaaattga aaaacaagaa tttcttataa cgcatggtat aatgccacat tagtttcact
     8821 ttctcatctc cttttaatct tttgttacat tttcattact aaaaatgata gcacaacgag
     8881 cctattaata gaacacttct ctcataaaaa agtcattatg attatagcgg gatgataaac
     8941 attgattttt tctttcaatc tagttgattt ctatacttat ttgctgtttt atgctagaat
     9001 aacaattaat tgttgtatat tttttataca gaaggtacgg ttgaaaaaat atccgaacag
     9061 ataagcaaaa acgaggcttg tctctttgac tcgaatccag tattctagtc aaagcatatg
     9121 aaaaaccagg tggcttgtcc cctcgttttg tgttgtctaa aaaaatcaac tagctaaatc
     9181 actgctttac gcagataaag gagacaaaac aatgatctat aaagtttatt atcaagaaac
     9241 aaaagtacgc aaccctaaac gggaagaaac aaaatctctt tacattgagg caaaaagcga
     9301 tgtagacgct cgccaacgtg tggaagaaaa taccccatac aatattgaat tcgttcaact
     9361 tctagaagga agtcatctag agtacgaaaa agaaaatgct gatttctcat tagtggagtt
     9421 ttaatatgaa actttccatc aaaaataacg agacaggcgt ctttgctatt ggtggattag
     9481 gtgaaatcgg gaaaaatacg tatggggtac aattccaaga tgaaatcatt ttgattgatg
     9541 ccgggatcaa attcccggaa gacgatttac ttggaattga ctatgtcatt cccgactaca
     9601 gctacatcat acaaaactta cacaaagtaa aagccttggt gattactcac gggcacgaag
     9661 accacatcgg cggaataccc tacttgctga aacaagcaaa tattcctatc tatgctggtc
     9721 ctctcgcatt agcgttgatc acaaacaaat tagatgagca tggcttgctt cgagatgcag
     9781 agctccatga gatcaatgaa gatacgatca tccgttttag aaagacttct gtcagctttt
     9841 tcagaacgac ccatagtatc ccagatgcat tagggatcgt ggtgaaaaca ccttctggaa
     9901 atatcgtagc gactggagac ttcaaattcg atttcactcc tgttggcgaa ccagcaaact
     9961 tgcataaaat ggctcgtatc ggggaagaag gagtcttgtg ccttttatct gatagtacaa
    10021 atgctgagat tccaacattt actaaatcag aaaaaacgat tgggcattct attttgaaga
    10081 tcttttcaaa aatcgaagga cgtatcattt ttgccagctt tgcttctaac atcttccgtt
    10141 tgcagcaagc agcagatgcc gctgtggcaa ctggtcgtaa aatcgctgtt ttcggacgaa
    10201 gcatggaaaa tgccatcgtc aacggtcaac gtttaggata tatcaaagtt ccagacggca
    10261 catttgtaga tgcacacgaa atcaatcgct tgcctgccaa tgaaacgatg atcctatgta
    10321 caggttctca aggagaagcg atggccgcgt tgagccgcat cgcgaatggt actcaccgtc
    10381 aaatccaaat ccagccagac gatacagtga tcttttcaag ttcacctatt cctggtaata
    10441 caacaagtgt caatcatttg atcaatttgc tgtctgaagc tggtgcagaa gtgattcacg
    10501 gaaaaatcaa taacatccat acttccggtc acggtggaca agaagaacaa aaattaatgc
    10561 ttcgtttgat gaagccaaaa tattttattc ctgtccacgg agaattccgc atgttgaaag
    10621 tacatgcttc tctagcacaa gatgtaggtg ttcctgcaga aaactgcttc atcatggaaa
    10681 atggcgatgt tttagcgtta actagcgatt ctgcgcgtcc tgctgggcat tttgtggctg
    10741 gagatgtgta tatcgatggt agcgggatcg gtgatattgg taatgtcgtt ttacgtgacc
    10801 gtcacctgct ttcagaagaa ggtcttgtat tagctgtcgc gacagtggat atcaagaaaa
    10861 aagaaatctt ggctggtcca gatattctct ctcgtggatt tgtctacatg cgagaatcag
    10921 gagaaatgat caatgaagct cagcgaatct tattccacgc tttaagagaa tgtttgaatg
    10981 gacctgactg ttcagaatat aaattgaaag aagcgatgac tggcgcgctt cagcctttct
    11041 tattcgaacg tacagaaaga catccaatga ttttgccaat ggtaatgaca ccagatgaag
    11101 aataaataag aggttatgaa atcagcacct tgtcctgaat ataagagaaa acaaatgaaa
    11161 aacatgagaa gccatttttg tttgttctta ttccttgata caaggtggct ctttcatagc
    11221 ctcttaaatt tatttacttt ttcatacaaa aaaatacctg ccacaagaga aattgtctct
    11281 tgtggcaggt atttttgtta ctcgctttgt tctctcgtaa aaattgatgt ctaccatcaa
    11341 tttgatttac agataaaatt aagcttttac aacgtttgca gcttgtgggc cgcggtctga
    11401 ttcttcaacg tcgaaagtca ctgcttgacc ttcttctaat gttttgaatc cgtcaccttg
    11461 gatagctgag aagtgaacga atacgtcgct tccatcttca cgtgagataa atccaaatcc
    11521 tttttcagcg ttgaaccatt ttactgttcc gttttccata ataatatcct cctcgtgcca
    11581 aaagcactaa tttaaattgt aacattgctc gtgtacgata gaggtgcaac agatgtttca
    11641 acttctattc ccaaacaaaa ttacaatata attgtacact tgcttcattt tttttgcaag
    11701 aaagcaactt acttctcttc catcattcct tcttctctca aactaaaata tcgtttgcgt
    11761 ccgatgatca agtggtccag tacagtgatc cccatcattt ctccacactt ttgtatcctt
    11821 ttggtaaaat tcatatcctg ctgcgatgga atttcatatt atcaagagta aattacaaaa
    11881 gaatcattta aatcaatact ttcactttga atacatgtat ttgaagtgga aggtacttat
    11941 ttcaaaaata gtaaaacctg tatcttaaat tacttaatag taacataaga tacaggctga
    12001 ttttttattc attgttattt atactaaagc acccgatagc tctgaaaaca atcacaaatt
    12061 caacttttca aagccacagc tttaagttat tttgtccaga caacccccat tgcccaaccc
    12121 attttatgga attggcatcc aggcaacaac ttttcatata aatcgtaata attttgttca
    12181 gataggtact tatctgaagc taaatgctca agccatgatt tagatgtgtt gtgattataa
    12241 attctaatcg cattttttat tccaaatttg aaacaatgcg gtaaaaactc ttggattgcc
    12301 cctaatttat aaacataggt aggcggtgtc tcgacttcag atacattatc aagaataact
    12361 attcttcctt cctcattcaa cagttccttc atttgctgta ttacgctggc tatatcatcc
    12421 aaatgatgaa aggttgttcg gcttacaata aaatcaaact tctcattaaa attaagttgt
    12481 tctgcattca tattcagata gaccgtattt gttagttgac gtttagattt ggcaagatcg
    12541 agcatttgat tagaaatatc aatccctacc acttcatcat aataacttgc taatttctcc
    12601 actaacaaac ccgagccaca tccgatatct aatgctctgc ctttctttgg agacatatta
    12661 gacacaaaga atgaataatc attcaaaagc tcattcacga aatcgtaatc ttctgcaacc
    12721 ttatcaaact gtgattctat tgtattcaaa aagatccccc attcctactt tatcgacatt
    12781 ctttcattac ttaccacttt agatgttttt tcgttgggga taaaacttcc ctttagacaa
    12841 ttttatccaa agacaataca acagtgcaac tttattaaag tcactgtcct ttatcgcagc
    12901 ctttactttt tagtaaagac agtggcttct cttatcaagt ttcaaaacat attattttga
    12961 agaaaacgtc catctgaagt gtcaagtgca aaattacata taaaggttta ttctaaaatg
    13021 aaaagatgat acaatcatat tcagttacat aaggaggttt caattatgtg caccagtatc
    13081 gcagtagtag aaattacttt atctcattca taatgaaaaa aatggaaagg agataaaagt
    13141 atgggtactt tttctatatt tgttattaat aaagttcgtt atcaaccaaa tcaaaattaa
    13201 ttggttataa tgaacgctta atgtcagttc attataacca gtaaggagaa ggttataatg
    13261 aaccagaaaa accctaaaga cacgcaaaat tttattactt ctaaaaagca tgtaaaagaa
    13321 atattgaatc acacgaatat cagtaaacaa gacaacgtaa tagaaatcgg atcaggaaaa
    13381 ggacatttta ccaaagagct agtcaaaatg agtcgatcag ttactgctat agaaattgat
    13441 ggaggcttat gtcaagtgac taaagaagcg gtaaacccct ctgagaatat aaaagtgatt
    13501 caaacggata ttctaaaatt ttccttccca aaacatataa actataagat atatggtaat
    13561 attccttata acatcagtac ggatattgtc aaaagaatta cctttgaaag tcaggctaaa
    13621 tatagctatc ttatcgttga gaagggattt gcgaaaagat tgcaaaatct gcaacgagct
    13681 ttgggtttac tattaatggt ggagatggat ataaaaatgc tcaaaaaagt accaccacta
    13741 tattttcatc ctaagccaag tgtagactct gtattgattg ttcttgaacg acatcaacca
    13801 ttgatttcaa agaaggacta caaaaagtat cgatcttttg tttataagtg ggtaaaccgt
    13861 gaatatcgtg ttcttttcac taaaaaccaa ttccgacagg ctttgaagca tgcaaatgtc
    13921 actaatatta ataaactatc gaaggaacaa tttctttcta ttttcaatag ttacaaattg
    13981 tttcactaaa ttaaagtaat aaagcgttct ctaatttcac aagaggacgc tttattcttc
    14041 ccaaaaattg ttcaatattt atcaataaat cagtagtttt aaaagtaagc acctgttatt
    14101 gcaataaaat tagcctaatt gagagaagtt tctatagaat ttttcatata cttaacgagt
    14161 gctttcacct ttgaatatag tccttcccac ttatcatcac actctccccg atagcctttt
    14221 ctagctatat ccagtaaagt tacatgctct ttaggtaaaa gaggtatagc ccattctgca
    14281 gcgacatctt tcgaggtaat ttcaccagta gtcactgttt gccacattcg agctagggtt
    14341 aaaattacat tacgctcatc accttttatc ccctcaatta gttctggcaa agaatcctta
    14401 attgctcttc gaatatctgt caaaggtacg gagacaagta tacttgaaga atcaggacca
    14461 aatagagaaa tactattctt tcttgcttgt gctaaaacaa tagccaaatc aggatcatag
    14521 cttggttcct gaatttgtcc attctcaaat tcacccctga gccactcacc gtatataaat
    14581 tctctttttg gaggatattg ccaagggaca acttcactcc tatttataac cgtaacttca
    14641 agtggtctaa cagaatccgt atttccaatc tttcctgata tagtcattag tctttctgtt
    14701 agtttttttc gagttaattg aggtaaacta tgattcacga cgactagaac atctacatcg
    14761 ctgttaatgc gtaaaccacc atttactgct gaaccaaata gatatactcc aactattgaa
    14821 cttccaaata aatcttttac gatttttaat gtttgaatcg cttgatttgg tatttttccg
    14881 ttaatcaaat tgctcatgat ttcacctcgt aactgattat gttcatataa agtttatatt
    14941 gatactcaat ttacttaccc tagattggac atatacctaa atgactgttc aataaagctg
    15001 accgttagcg tttaagtaca tcctttcaca atttgtctac agattaataa ttattcttta
    15061 ttatacagat ctccatataa tttttgaatt tggttctgta attttttatt ttctttttct
    15121 aattccatta ctcttctttt taaggtttta ataaggattt cctccgaacg agaacttttc
    15181 ttgggttttg agactacatt tgctgttatt tgacgctcac gaagggattc gattctttgc
    15241 ctaatatcgt gttccttata aagccatgat ttagaaacat tagcttcctt tgctattgaa
    15301 ttaaaattaa taactttacc ttcaatcgaa aatttagaaa tcgctttgtc tactttttcc
    15361 cttgtctttt ttgatttctg cttcgccaaa cgtacaattt ctgttgtatt tctaacttgt
    15421 ttatccattg ataattaccc cgtcaaactt ccaatgattt gttctaaacg ctctttaaca
    15481 cggctattag tctctacttg tctttgccat tgtttatcct tagctatggc taataactct
    15541 tctgtacgct ctaactgttc ttcgtgctgt ggtaagaatt gcttactggt acagaagtga
    15601 gtgcaatcta agcatgcatt cgcatgtgga caaccacctg ctactactgg caatctacaa
    15661 taaccatttg gaagcacttg tgcatttata tttttcttga accattgaag ctctacatca
    15721 tcgacttcat tatcttcatc tagatcaagc acatctccat tattggtaac cagtttttcc
    15781 tgaaatttag taaattcatt ttttagagtt tcatcaaaga tatgagcgta tctgcttgtc
    15841 atttctgggc tttcatgccc caaaaatttc tgcacaatat gctggggcat cccgttgtta
    15901 atcattcttg ttcctactgt atggcgaaag gcatgggcat ggaatctata aatctcacct
    15961 gatttatcca ctatattttg ctcataagct aatttattta actcacctct aaatgtttct
    16021 tgttttaatg gcgatccatc ttttcttgga aagaggtatt cactatctgg aaattcctct
    16081 gaaactttat cttcccgaac tttaataagt aaagctacct ctttagatat tggaactata
    16141 tgctcctttt tcattttcca ttgataatac tttaaaaaga aatctccatc tttgtcctct
    16201 aatagacagc cttttttcaa ggtgcacaat tcacttatcc tcattccaca ttcttgaaca
    16261 atcatagtca tcgtagctat atattcgggt aatttatcaa gatgactgtt caattgctct
    16321 aggacgaatt catctataaa gcgtggtttt gctcttggta ttttcggata gtcctcagaa
    16381 taaattaata ttttggaagg aacatcatcc cattctagcc taagaagggt actaaatagt
    16441 ccttccaata tagagatcct cccagttatt gtactaggtt ttattcccat catgtttagt
    16501 tcacttaaat atgcttcaat ttccactctc gttaattggt gtactctctg aacttgttta
    16561 aatttcatgt ccagaaaatt aaagaactct ttaagtcttt gggcaatatc acttacatag
    16621 gaaaagctat ccatgttcaa tctcaactta caatatcttt ttacaagttg tttaaaatat
    16681 gtattccgaa accctttaaa gttaattgta tattcatatt gtgttgggtt aaccttatca
    16741 tctggcaaag gtaagttacg tctatcccaa acgtctttat cccactcctc tccatcaaaa
    16801 taaaagttct cataaaactc cataaattgt tttagattag taacatagta ggaattagct
    16861 tttacaggtg ttttttcttg attagcagta atcttataat tagtagtggt aattctaaca
    16921 ccccgttttg tcaaataagt tctatactcc gtcattgctt tttcaatagg aacttcagta
    16981 attgaagtaa tgctaggata ctttaaatct aagaaatcta acattttatt aattactgtt
    17041 ccttttctaa tccagacagt ttttgcattc catattccat tgtttaaatg gtaaaaataa
    17101 aaatatttca attctgttct taaccacaga tttttaacac gttcaaaacg aacccaacga
    17161 ttccttaaag caggattctt acttaattct atggcagaag gatgtggaca ttttcttata
    17221 tcccaactat tattagccca aaacccctgc atttcttcat tcattacagc tatttttttg
    17281 ctaatctcac tctgactaat aattttcctt ttactagaag cattcatttc ttatgctcct
    17341 ttctctcgag gtatttatta aactcatttt tcatatcctg atctgaaaga tgaacatagg
    17401 tatttaacgt tgtctgaaca tgtgcgtgac ctaatctctt ttgaacgaac gcaacatccc
    17461 atccttccct aattagctgc gttgcgtgag tgtggcgaag catatgtgat gtaaattcta
    17521 ttccagtcct tttaactatt cttctaacta gatcaagaac actttggtac tttagtggtt
    17581 tcccaaaata gccttctttt aaggaaataa aaacataatc atgctccaat tcctcactat
    17641 actcatatat caagtaatct gtataaagtg acataagttc tttactcaca tgtattgttc
    17701 tttccttcct taatttaata taagcttcat taacattaac atctctaggt gttaaatgga
    17761 tttgattgtc ccaagtgaca atatcttcaa gcctaagcga taacacttca ccgattctta
    17821 aaccaccctc atacataagc attaaaatta atttatctct tttcgtatga caagcatcaa
    17881 taatttgctt aacttcctct gatctcaatg ttcttatctg tttcttttta acccttaact
    17941 ttaagacatt cttttggtat ctacccttat taacatgatg taaaaatcct ttgaaatttc
    18001 ttccctttgc ttgtttaaat acatcaattg atttaaattc tcctaatcta cttaaataat
    18061 caagaaaact cataactaca tttaaaattg tattcactgt cgtttcttct cttatggctt
    18121 tttttgactg aagatcaatt acatttgatg ctgaaggata tctcaaccaa cctacgaagt
    18181 ctgctaacaa ctcaaagtta atatcattaa gaataacacc tctctgttcc atgaactcgt
    18241 acagcaactt taaatgatag cagtatgctt taatggtatt aggagactta ccagtattat
    18301 ctaagtattt aataaatttc attactggtt ctattagctg gtattcttta tctagtaata
    18361 aatacaatgg atacggctta ttctccactt ctatcctttg aaccttcaca tgttcccacc
    18421 tctttaaata ctttaactac tacaaattat aaaccttgtt aatttttatt taaagtaaaa
    18481 tatcccttta atatctcctc t
//
LOCUS       DAZKBH010000049        18407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000049 DAZKBH010000000
VERSION     DAZKBH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.049"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(110..565)
                     /locus_tag="ACG8OO_001585"
     CDS             complement(110..565)
                     /locus_tag="ACG8OO_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045469.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="ACG8OO_001586"
     CDS             complement(719..1927)
                     /locus_tag="ACG8OO_001586"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFP9045470.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="ACG8OO_001587"
     CDS             complement(2039..2371)
                     /locus_tag="ACG8OO_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9045471.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="ACG8OO_001588"
     CDS             2519..3394
                     /locus_tag="ACG8OO_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9045472.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="ACG8OO_001589"
     CDS             complement(3476..4057)
                     /locus_tag="ACG8OO_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFP9045473.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="ACG8OO_001590"
     CDS             complement(4063..5322)
                     /locus_tag="ACG8OO_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9045474.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="ACG8OO_001591"
     CDS             complement(5324..6370)
                     /locus_tag="ACG8OO_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045475.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="ACG8OO_001592"
     CDS             complement(6605..6880)
                     /locus_tag="ACG8OO_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFP9045476.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACG8OO_001593"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACG8OO_001593"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9045477.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ACG8OO_001594"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ACG8OO_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9045478.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /gene="cmk"
                     /locus_tag="ACG8OO_001595"
     CDS             complement(9979..10659)
                     /gene="cmk"
                     /locus_tag="ACG8OO_001595"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9045479.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="ACG8OO_001596"
     CDS             complement(10708..11343)
                     /locus_tag="ACG8OO_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFP9045480.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="ACG8OO_001597"
     CDS             complement(11409..12812)
                     /locus_tag="ACG8OO_001597"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9045481.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="ACG8OO_001598"
     CDS             complement(12799..13830)
                     /locus_tag="ACG8OO_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045482.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="ACG8OO_001599"
     CDS             13861..14082
                     /locus_tag="ACG8OO_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFP9045483.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="ACG8OO_001600"
     CDS             complement(14247..14984)
                     /locus_tag="ACG8OO_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFP9045484.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="ACG8OO_001601"
     CDS             complement(15137..16477)
                     /locus_tag="ACG8OO_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9045485.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="ACG8OO_001602"
     CDS             complement(16714..18237)
                     /locus_tag="ACG8OO_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP9045486.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5187 a         3924 c         3107 g         6189 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttct
//
LOCUS       DAZKBH010000050        17406 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000050 DAZKBH010000000
VERSION     DAZKBH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.050"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..555
                     /locus_tag="ACG8OO_001603"
     CDS             118..555
                     /locus_tag="ACG8OO_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045487.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            650..1534
                     /locus_tag="ACG8OO_001604"
     CDS             650..1534
                     /locus_tag="ACG8OO_001604"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9045488.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1758..4925
                     /locus_tag="ACG8OO_001605"
     CDS             1758..4925
                     /locus_tag="ACG8OO_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9045489.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4993..5490
                     /locus_tag="ACG8OO_001606"
     CDS             4993..5490
                     /locus_tag="ACG8OO_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFP9045490.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5509..5973
                     /locus_tag="ACG8OO_001607"
                     /pseudo
     CDS             5509..5973
                     /locus_tag="ACG8OO_001607"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5970..6728
                     /locus_tag="ACG8OO_001608"
     CDS             5970..6728
                     /locus_tag="ACG8OO_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9045491.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6814..7554)
                     /locus_tag="ACG8OO_001609"
     CDS             complement(6814..7554)
                     /locus_tag="ACG8OO_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9045492.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7615..8868
                     /locus_tag="ACG8OO_001610"
     CDS             7615..8868
                     /locus_tag="ACG8OO_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9045493.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9190..9417
                     /locus_tag="ACG8OO_001611"
     CDS             9190..9417
                     /locus_tag="ACG8OO_001611"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9045494.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9540..9866
                     /locus_tag="ACG8OO_001612"
     CDS             9540..9866
                     /locus_tag="ACG8OO_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9045495.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9934..10647
                     /locus_tag="ACG8OO_001613"
     CDS             9934..10647
                     /locus_tag="ACG8OO_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP9045496.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10957..11808
                     /gene="licT"
                     /locus_tag="ACG8OO_001614"
     CDS             10957..11808
                     /gene="licT"
                     /locus_tag="ACG8OO_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFP9045497.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11929..13794
                     /locus_tag="ACG8OO_001615"
     CDS             11929..13794
                     /locus_tag="ACG8OO_001615"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9045498.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13808..15251
                     /locus_tag="ACG8OO_001616"
                     /pseudo
     CDS             13808..15251
                     /locus_tag="ACG8OO_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15359..16060
                     /locus_tag="ACG8OO_001617"
     CDS             15359..16060
                     /locus_tag="ACG8OO_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9045499.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16186..16698
                     /locus_tag="ACG8OO_001618"
                     /pseudo
     CDS             16186..16698
                     /locus_tag="ACG8OO_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(17096..17305)
                     /locus_tag="ACG8OO_001619"
     CDS             complement(17096..17305)
                     /locus_tag="ACG8OO_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFP9045500.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         5810 a         2919 c         3729 g         4948 t
ORIGIN      
        1 tttagttctt tttattttaa aaattttttt tacttagaat ccagcgaccc tagatatttt
       61 tctgtataat cttaaataaa gaaaacaata tcaaaaaatg cacgtagaag tagataaatg
      121 gagtggtttt gcaaatttct tttatttcta gaagttattc acgtatacgg atttgttttt
      181 ctcacaaata aaaaggcagg aagtacaatg gatcatttgt atataaaata tattcatcaa
      241 ttttccgttt tggtgttagg cacactaatt atagcgggga ttgtcgcatt tattattcaa
      301 ggccgtgaca tattaacagt acatcaagag tttttcagtt atgcatttgg tattcctctg
      361 atcgctataa ggtctgataa caaaaagtgg aagaaacgct attacattct cttgctattt
      421 ttgtgttttt atctgccacc taagattttt actttattag gtatacaatg cctatcattt
      481 tcttttattt cctatatcat tgttttaatt ttcctcagtt tcatagtatg gatgttttat
      541 cttgtcaaaa aataaatgaa tgtttcatga acagtacttc atcacagcct aatttttttc
      601 tttatgctat cgttatatta aagaaatcaa tcagaaggga aagaaagtga tgaaaaaagt
      661 tttattagtg gtaaacccca gttcaggcgg tgaacaagca aaggaatttg agcaattagc
      721 gatagcaaaa ttggaatcgg tgtttgatga agtggtcgtc ttgcatacaa aaaaagcagg
      781 ggatgcaaaa aactttactc gcgaagcggc tacggaaggg tatcatagtg tgtttgtcat
      841 gggcggagat ggaacagtca acgaaggaat cagtggaatc gctgaacaag aacatcggcc
      901 aaatttcgga tttttcccat tgggtacggt gaatgacttg gctcgagcat tagggatacc
      961 gctagaacct gaggaagcga tcaatcattt ttcgattgaa tcagtcaagc cattggatat
     1021 tggaaaaata aatgacgact attttatgaa tgtcgtcgca attggctcta tccctgaatc
     1081 aatcaatgat gtagatgcgg agaagaagac gaaatttgga aaactagctt atttcatgtc
     1141 aggaatcaaa cagttagctt ccacacaatc ctactcgttt cacgtagaag ttgatggaaa
     1201 gaaggaagaa attgagagca gtaccttgct gataggacta actaactcag tcggcggttt
     1261 tgaaacatta cttccaaatg caaaagtgga tgatggtaag ctccatttgg tttatttaaa
     1321 agattcttct ttgatcgata cgcttaaaac attgccagac ctattaaaag gagtagatga
     1381 atctacgaaa aatctagcct accttacttg tgagcaatta gttgtgtcat tagctgatga
     1441 ggaagaattg acgacgaatg ttgatggaga tgaaggggat aaattacctg tgacaatcaa
     1501 agtattgccc tcacatttac aggtttattg ttaaaatgga ggttgtgacc gaggagttgg
     1561 cgggagaaca ccgcttgttc gggataaggg actgtgacaa gacttttgtc acagcctttt
     1621 ttttgtacaa aaaagaaatt tacacctaat agaaaaattt ttccataaaa atacatattt
     1681 taaatatgaa agtacaattg gaaatagatt aaaacgataa aataaaattg tcaaaacgtc
     1741 agagaggatg gtggagaatg aatcaatgtg taaaaaagct gattgcgtcg attttgtttg
     1801 tcttccctct cttgatggtg acatccgttt ttgccgatgt agagaaaaca aacgaatcaa
     1861 cgcaatcaga gaatcaaagt tttgacaaga ctactttttc agaagaatcg attacttctg
     1921 aaactacaga atctcaaagt gaggcttcta ctaataaaag gcaagaagaa aataagacaa
     1981 gtgagacctc agaagtagct gaacaaacgt ctgatagcag tgaagcggca ctggcaagag
     2041 ctgtttcagc ggatgggtac agcgaagctg ccacaacaga agaactggcg cagcttctcg
     2101 cagacgaatc agtaactaag atccgactga ttcagccttt gactttagac agggaattag
     2161 agatcaaacg agatatcgtt attgattttg gtggattcgc tcacaatttc ggcacacacc
     2221 atatttatat caatgagata cctcagtcga tcgagttcca gaattttaaa ggaacagctg
     2281 cacatccagg tggattgaca ccaaatatcg atggaaatgc cattatcatt gcttatatga
     2341 ccgatttttg gggaagcaga tatcatttta cgggagaaat aaaatttacc gggacacttg
     2401 atttgtatga tgggtccaaa ttaggactga tttacgctcc tcgtgcaact gtcacattag
     2461 atggtgtttc cggtgtgttg gatgtccaac cagtgaagga aggactgaat gccgctccgg
     2521 gtaaagccta tttttgtaga agctatcaat tgaatgtgat caatggtagt caactttatg
     2581 gaccgtatct agggaaattc tacggttttc tagattcagg agatgagact ggtagtggaa
     2641 aaacagcgcc aggaatccat atcttaagcg gttcaaaggt ctctcttgat tatgatcggg
     2701 cagatggcgt tagcgatgaa ggagaggcag tcgatacgct acctggtaat gtcatgtttg
     2761 aagtcagtgg ttcgggaagc gaattttcgg taaatacaaa gatcaatatt accaatgaca
     2821 acaatcgcgg aatcatccaa atgagaggtt caggatcgaa agtcactgta tcgaatggtg
     2881 ggaaaatcac catcaataca gcaactacgg gcggatttcg cttgcaagga gattctagtc
     2941 aaatccatgt atcaagtggt ggacaaatct cggtcaaaat ggctggagat gacgatcaac
     3001 ccgggaacaa tgggatgcgt tttgtaggaa aaggcctttc tttgatcgta gaaggagccg
     3061 gatcagttat cgacatcgat aagcagagtg ggcgttccag cgcagttcgt tttgagaatg
     3121 gtacgcaaaa gctgacagta accaatggag gctcattgag ggtaagaaat acaggtgatg
     3181 gcaaatctta cgtgaacaga ctcaatcaag gaaatcaagc gatccaattt tatgacgctt
     3241 ctggttttct agaaagtcca ggttcagctg attttacagt cactggagaa aaatcaaata
     3301 tcgatatacg cgcagattcc ggagcaactg tagatacaac tggagatatt gatttaaatt
     3361 ttacggcagg tgaaaaaact tatttagtca tggtcgggaa aacaggggaa gataagaccg
     3421 gcatcatcag tggggatgtg ttgaatgtgg acttagaatc tcctacctat tttgatttcc
     3481 aaaacaaacg gattggaagc gagaccaatg gcataggcgg ctgggtattc caaggaaatg
     3541 gagattcttc tttaaagatc gcccattcag aaatcgcttt atggcgaaaa acaggcgcaa
     3601 cattcgataa ctttgaaaaa gacccggatt atttttctga tatgacagac ttatcgatca
     3661 atgggaccga cttaggcaat ttcgtatcaa gccctagtgc aggtttaaca aatgaatttg
     3721 ctcagacagg tgccggaatg aaaaattata atcgaatcag tgccaataat cagctcccga
     3781 tgattgaatc cttaagagtg ccaacgaatg cagataaaaa aatctatggg catgtcgttg
     3841 ttcccgaagg tgtcgaggca aagccaagag atgcttggac aggcgaagta gaagtgactt
     3901 tacgtattac ttacgcagat acgacgaaat catctgtaga aattactgct ctcacgcaag
     3961 gacaaacaga tgaaaatgaa ggggcaggat ataacccatg gggagaaggc gaacaaggcg
     4021 gcttgttcga agttcaagta cccaatgatg agagattaaa aacggaggat caagtgcaag
     4081 ttgttgctgc taaaaaagta agaggtggcc aagaagtgac agaactggac tctgccaaaa
     4141 cgacagtcga tgtgattcct cctgaacctg ctaatttaag taccacattg atcaatgcag
     4201 cttctcgcac gatcacaggt acaggtgaag caggagcctt ggtttcattg aaaaaaaatc
     4261 aagctgtttt gtcagaaaca actattgatg atacagggga atttgttctt tctatcccag
     4321 aaaatcaact ttttgcagga gatgtattag aaattgtatt aaacgaccaa gcaggagaag
     4381 ctactgctaa gggtgtgcag aacaagccaa gtaccaacga tgaaattggg aatcacaatc
     4441 caagtgatac gccagttgtt tatcatgatg ccccgccttt tgcacctgct acgaagatcg
     4501 tagtagaagg tggtctctca tttcttgccc cttccaaact cgattttggt cagatcaaag
     4561 caactggttt gacacaagaa gcatttggcc aaatggatat acaggaaaaa ttatggattt
     4621 atgatcagcg agaaacgaaa aatccgtgga tcttaacgat aaaattatct cagcctttca
     4681 caaatgatgc aggattttct atgcaggatg tcctatattt caagaaagga gaggagtatc
     4741 aattagtgaa tgaagaagaa ctggaaattg tcagaggaca aaattcaacg aatgtgcaag
     4801 cagattattc agaatattta aataacggtc gggcgtttaa acttgtctta accaaagaaa
     4861 atcagatagc tggtaattac tcagcagaga taacatggac attagcagat gcgccgacag
     4921 aataaagatc aaggaggatg attgatgaat cgaatatgga ataaaagtca tcgaaacaac
     4981 tgctctttac ttatgatcct tctccttgga ttcatccttt ggcagacacc tgtccatgcc
     5041 tccgaattca attttgcggt agattttgaa ttaccggcta atcagattga taagaacaaa
     5101 acatatataa atgtaaggat ggagcctggg aaaacacagg aaatccccta cacggtcttt
     5161 aaccgaacag ataaatcagt aacagttgag acattgatcc actccgctac gacgaattca
     5221 aacggtgtga tcgaatatgg agaatcaaaa agtaaatttg atgaaacatt aagttatgat
     5281 atgaaaaact tagcaaaaac agtgggagaa ataactgttc ctcctcaaag tgcggtcact
     5341 gagacacttt cactgaaagc gccagaaaaa gagctggctg gtgtggtcgc aggagggatc
     5401 acgtttcaag aaaaaaaaga gaacgtttct aacaaaagta cagttggtgt cgtcaatcag
     5461 ttttctcaag tgaaagcaat cttgatttga aatagcgaaa aagagtcttc cccagatttg
     5521 aaattaaaaa aagcttatgc ggatcaaagc gatggaagaa atgtgattaa ggcgaatctt
     5581 caaaatccaa atgcttctta tatgttcggc ttgactacat cggttgaaat ctacaaaaaa
     5641 gaaacaaaag agccttacct ttcaataaaa aaagaaaaaa tagatgtagc acccaataca
     5701 aattttgatt tacttgtccc attagaagga aaaaaattag ctccgggaca ttatcaagcg
     5761 gttgtgaagg gtgcttggaa agaaaaacgc tggaatctcc ataccgattt tgaaataact
     5821 gatgatgcag cggaccaatt gaatgaaaag gatattgatt taaaaaatca gccagaaaca
     5881 tttgactggc gatggctgat acttgttgga gtcattctta ttggcttatt tgctggatta
     5941 tttattttaa aagaaagaag gaaaaagaaa tgaaaaaaac aattgcatta agtaccgttc
     6001 tagctggaag tctattatta tgtacacaaa gtgtttttgc tgagaataag agctcagcca
     6061 ctacagaagg aacgatttct gtgaccaaac cagatgctgc aaatcctgaa gacgttgttc
     6121 cagttcctcc tgttgatcca aatgatcctg atactccttt tacacctgac gaaccaaatc
     6181 agggaacatc cggattactg acgattgacc aagctccaga tcttgatttt ggaacaatca
     6241 agttaggtac agcaaaaaca gtatatgctg cattacaaag cggaacggac agtactggag
     6301 cacaaacaca agtacagaat ttcgtacaag taacggataa aagcggaaat tatgccggtt
     6361 ggaaattatc agtcaaacga acagaattta ttggtacaac atctgatacg aataaactaa
     6421 ctggttcaaa ggtgaaattc aaaaacgccg tgattcgaaa tagcgcaact aatagcagtg
     6481 gaatgccatc taccgtttct ggaactggcg ctgaaggaat cgaaatccca gcagatcagg
     6541 cagtagatct agtaactgca ggagcaaatg aagggattgg cacatggatt tattcattag
     6601 gagagaatgt ggaacaaggg agacaatcag tggaattatt tgttcctgta tccaattatg
     6661 ctgtagacaa ctacgtttca acattcacat ggtcgttaac agatgctcct acagaagagg
     6721 aagcgtaaaa tcatatcaac gatttcaggg agtggagccg attcaaggcc cactccctga
     6781 aatctgctat gcttacgtct cagctcttat tcatcaactc gaagcagttt tttggaagtc
     6841 atggaaacgt tcctttgtct tttcaaagat ctttcgacca acgagtaagg agtcaccgtt
     6901 gaaaaattct aacacataat gagcagaatc aattttctgc aattgcgata agttgataca
     6961 gtaagatttt aaaagtaaaa agtaagcata cttttctaac tctgtcttca attgttttaa
     7021 agaagtattc actaatttat cttctgtagt tgttttgacc ataacttggt ttttcaaacc
     7081 tttgatactt tcaatatata aaatttcccg aggatacagg acgagacttc cacctttttg
     7141 atcaataaag attttatcga catttacgat ctgctgttct ttccaattga tctgttctag
     7201 cacttgacga atttgttcag ataaattcct gtcttttctg atatatccaa tcggaaggat
     7261 tttccgattg atgatctcta ctgcaaaatt cgtataagcg gtgaaaaaaa caatattact
     7321 atgtgcattt tttttacgta tttgttcggc gacatcaata cctgtgagtg attgcttcaa
     7381 gtcgatatcg ataaagaaaa catcgtgatt atcaatagtg cccaactgga tcaactgatt
     7441 gatttgagag atagaagtga gctgcacgat attctcgata tgatgattga ccaattctct
     7501 gatgatccat tcgctgtgaa actggttatc ttctatgata aaatactttt tcatatgcag
     7561 gctcctatta aaaaaatatg tgtacatttt gaaacaaaac aatggaggaa taagatggtc
     7621 gcaacagtga cactttattt gacctttact ttgattttag atttaggtat ctatttattc
     7681 ttttcgaaac aactgctagt aaaaaacagc tttggttgta tagctttgat ggtggtgaca
     7741 cttttacttc ctatccctaa agtgagcttt ccttacctag ttggcttatt attgatgttt
     7801 cttttttatt ttactacgaa aaacctgttt atcagctgtt tgatcagtaa tctttttttt
     7861 gctatcactg gactcagctt ttttctggtt tttgatctga tggattattt aggaaagaga
     7921 catcttggaa cagcaagttt ttttgcacta cttatcgcat ttattttcta tctattagta
     7981 aaaagtatag acaaacagac gagatttctc aacttgcttt ttacttatcc aactgaacac
     8041 tattttggag gtatttgtat tttattaagc tggggcgtga tttacttggt attgggaagt
     8101 gttcacttcc gctcatctaa ttatctgctt ctatctcttt tgtcgatagt catcagtttt
     8161 ttttctttgt ttttttccat tatgttgatc agtttccaag taaaagaaag acaacatcag
     8221 gaatcgcttc aactgtatca aataaatgaa gaatattacc atcatctaga ggaattcaaa
     8281 catgattata aaagtctact gttttcatta aaaattactc ttcaggaaaa taaaaaagaa
     8341 acgttggaat ttttagaaat attagaagat cattctgcag ctatttttga aaaaccgcaa
     8401 atacaacagc taacaaatat tgtatcccca gctgtcaggg gtgtcttttt aaaatttcta
     8461 gaaaaagcag aatcggagaa aatcccgatc caagtagtta ttcctgtcga agtgaaaaca
     8521 atcccgattg atctggttgt ttttattcgc tgtttatcta tttttatttc taacgcattc
     8581 gatcatcgtg tagccgatca gagtccgatt acgatcaatc tgctggaaga aaaagaggga
     8641 tcacgtttta gtatagcgaa caaggcaaat ccaacgaatt tgacgctatc ggagatggtc
     8701 aaaagaggaa aaacctctaa aaaaaatggc gggttaggtc tatacagtgc gaaaaaaatg
     8761 ttggatgttt acgaaaatgc agaactaaaa atcgaatttt caaaacagaa tcactatttt
     8821 tttgtagaaa tgtatctggc atctagaaag agcaaatcag cctactgact tactgtcaaa
     8881 ggataagttt gaggaaagtc tacacggaga tagagacaac aacacagcat attcaaaaga
     8941 aacagaagcg tgatgaccgt tgtcaccctt ctgtttcttt tgctatgtag ttaaacaaag
     9001 tagttgacac ttccgctact tagtattagt acgaaagtat acttcatgat actaagtaga
     9061 taagaagtga aaagcggaac ccacgatcgt caaaatgcta actgctttgc ttacaccaga
     9121 taaagggaag agcaatatcg cttattttag gcgtggagaa gcaagcaaat ccctacgatc
     9181 acaaaaaaaa tgctgaccca gcagttaaga gagttagaac aagatgaaag tatccatcga
     9241 aaagcctata accaagtccc gcctaaagta gagtactcgc tcaccgaaga aggaaaatca
     9301 ttaagagaga ttctagtagc gttgtctgtt tgggaagaag aacgtatcga aaaacaaaaa
     9361 gcctcaaggg aagaagacac gctactgcat acacatggtg gctacctaaa ttattgattt
     9421 tctatgacat ttattttaaa taaaagaaaa caggagtttt tttaaaagca tcatctattt
     9481 tttatgtaaa aaaatggtat gattaatcaa gtaagaagtg attggaagaa ggcaagcaaa
     9541 tgaatagtat ggaaaaagtc aaccagttta gagatgaacg taattggcga caatttcata
     9601 acgaaaaaga tttggctatc tctatttctt tggaagcatc tgaattgtta gaactttttc
     9661 aatggaaaac gccggaagaa gtaaaagaaa cacaattaga acgaatcaaa gaagaactgg
     9721 cagatgtatt gatctattca tacatgatgg cagacaacct tcacctggat ctagatgaga
     9781 tcattgaaga aaaattgatc aaaaataatt tgaaatatcc tgtagaaaaa agcgcaggtg
     9841 tacgaaaaaa atacacagag ttgtagaaat gtgtcattcg gctgttggaa agagcaaaag
     9901 aaaaacagta aaatgagtct ggagggagag aaaatgaaaa aaaaaggaaa agcaaaaaga
     9961 tggctagtca atatcctctt gttcctcttg ttattagtag gactagcttt gattttcaat
    10021 gaacagatca aggattattt tgtcagagag acaggagata aatatgctat agcgaatgta
    10081 acaaaagaag atctgaaaaa gaataacgat aaagatgtca gttttgactt tgatgcggta
    10141 gaaccaatga cgacagaagg ggtcatgagg tcgcaaatga gaggaacaga tctaccagtg
    10201 attgctagta tcgcagtccc ttcagtttca atcaatttac cggttttcaa aggcttggat
    10261 aatacgtcac tactttacgg agcaggaaca ttatctccag atcaagaaat gggaaaagga
    10321 aattatgcct tagcaagcca tcgtgcaacg aaccctgaat tgctgtttac accgctagaa
    10381 aatttagaga tgggtgccaa gatctatttg acagatttag aaaatgtgta tacatacaaa
    10441 acatttttta aagaaaaagt tgctcctaca gatacccaat tactgaatga agtcgaggga
    10501 aaagaaattg tcacactaat tacttgtggc gacatggacg ccgtcacgcg actagttgta
    10561 caaggagaac tggaaagtgt tacatcgata aaagatgcga cagatgatat gagatctgct
    10621 ttcaatctag agacaaaaac attttaggat actgggacat aagtcgttac agattaaaaa
    10681 cagccaaagg atatgaatcg ataaggaaaa accaatttgc tgaaaaaaag agaaacgcaa
    10741 agaggtgcat ctctgatttg gttgttttct agatagactt gtgttccagt ttttttattt
    10801 tttatatgaa aaggcttgca taaataaaaa ttcgatggta tgatgatttt gaataagaaa
    10861 tggattgtta ctgtacggca ggcaaaacca gaattcagct aagaaacagt aggcgaattc
    10921 taggtttttt tatttttctt gagagggcga agcaaaatga aaattgataa aattttgaat
    10981 aacaatgtcg tcatttcgaa aaatggattc ggggaagaag tcgtttgtat gggaaaagga
    11041 ctagcttttc aaaaaaagat tggggacgag atttcgccag aagcggtcca aaaagaattt
    11101 gttttaagag attcttttgc taaaaaccaa ttccagcagc tgatggctga tattccggca
    11161 gaggagatcg aatgggtcaa acaagtagtc gaactggctg aagataaact aaaagttgaa
    11221 ttttcgccta atatttatct gacattgacc gatcatcttc attatgcgat cacacgagca
    11281 aaagaaaata tccaactgcc taatccatta ttatttgaaa caaaaaaatt ttatccgaaa
    11341 gaatatcaag cagcgaaaga ggctcttttg ttcgtgaaac aaaagacggg tgtggaactt
    11401 tctgaggatg aagccggttt tattgccttt cattttgtga atagccaaca aggaaacgaa
    11461 gacatgcaag tgacgatgac cgctaccaca atggtaagag atatactgag tatcatcagt
    11521 aaattcttcg gcatcgtgtt cgatgaagaa tctttgaatt atcagcgtat cattactcat
    11581 ctgcagtttt ttgcccagcg ttatctcaaa ggggaaggat ctgacgagca agatgaattt
    11641 ctctacgcat tagttcaagg aaagtatcca aaagcgtttc attgtgtcga acggatcaat
    11701 gagtatcttc gacaaacgca aaaacaggaa atggggatcg cagagcagat ttatttaacg
    11761 attcatattc aaagagtagt aagtgagaaa aaaagcatcc aatcataagg attgttactg
    11821 tatggcaggc aaaacctgga ttgaaaaaat aggttcacga atgtttgtga ctgttttttt
    11881 caatctgggt ttttattttt taaataaaaa agaaagaagg aaaaaacaat ggataatcaa
    11941 gcagttggtc gtcgtgtatg ggaagcggta ggaggacaaa agaatgtcaa aagtttagta
    12001 cattgtgcta cgcggttgcg attcagatta aaagatgaat ctttagcaga tacgcaaaag
    12061 ttaaaagaag acccggacgt gatccaagtc gtacaaagtg gcggtcagta tcaagtagtg
    12121 attggcagta atgtagcaga tgtttatcaa tcgatcgtgg atgaggaagg tcttgcagtt
    12181 tcggaggatg gaaaagaaga gtccaaaaat attttgaatc gtctgatcga tattatctct
    12241 agtatattta caccttttct aggggcgatg gcagccgccg gggtattgaa aggattctta
    12301 tcattggcaa cggtgatggg ctggctgaca gctgatagcg gtacttatca aatattgttt
    12361 gctgcagcag atggtgtctt taccttctta cctgtcatgc ttgcttttac agcagctaaa
    12421 aagttcaaag ccaaccagtt tttagcagta gcaattgcta tggcacttgt gtatccagct
    12481 atcacagcag ttgctggtgc gggagaagct atcagtttct ttgggatccc agtgattctt
    12541 tcaccatctg gttatacttc ttctgtcata ccaatcattc tagccgtatg ggttcaaagt
    12601 aaattagagc cattcgtaaa aaaagtgatt ccgcaatttc tgcaaatgat tttagttccg
    12661 cttgtcgttt tagtagtgat ggttccatta acatttcttg ctttaggacc aatcgggacg
    12721 gttgccggta atgctttagg cggtctcttc aactcgattt atggatttag tccaatcgta
    12781 gcaggtctaa tcatgggaag tttatggcaa gtattcgtga tgttcggcat gcattgggga
    12841 ttcgtaccga tcatgttttt gaatatcgaa caatatgggt ttgatgtatt gatgccaatg
    12901 cttcttccag ccatcttggc acaaggtggg gctgctttag ctgtagcatt gcgaacaaaa
    12961 gatacgaaac ttcgagcatt agggatttct tctacagtga cttcgttatt tggaatcact
    13021 gaaccaactg tgtatggtgt gacattacct ttgaagaaac cattcattgc cgcttgtatt
    13081 tctggaggta ttggtggagc tattatcgga ttttccggag taaaagcttt ctccagcagt
    13141 ttggtcagct tattgacgat tccgactttc attaatacgg tggatggtgt agagtcaaat
    13201 gtgacagtag cagtaatagc tacaggaatt gcttttgttc ttgcattcgt aggtacattg
    13261 attttaggat ttgacgaaca aacacaagac aatcaattag aaaacaaaca tgcgaatgca
    13321 ggaaaaccga ttacttcagc acggcatacc ttaaaaagtc ctttaacggg taaagtactg
    13381 ccattgagtg aagtaccaga ccaagttttt tcttctggcg tgatgggaaa aggtatcgct
    13441 attgatccag aagtaggaga actagttgca cctgcagacg gagagatcac gactattttt
    13501 ccaactggac atgctgtagg gatcacaacg actgacggag cggagatttt gattcatatc
    13561 gggatggata cagtagaatt aaatgggaat ggctttgaga tcttagtcaa acaaggggat
    13621 ctcgtcaaag caggagattt attgattcgt tttgacatcg aagcaattag agcagctggc
    13681 tatagcgtaa tcactccagt tgtcattacg aatactgatg catttgccga tatacttgaa
    13741 ttagaccaaa aagagattat tgcaaacgaa gatgttctgg caattgtaaa ataaggagga
    13801 aatcaggatg caatcacgtt tttcagaaaa atttttatgg ggtggagcaa ctgctgccaa
    13861 atcaattaga aggggcttat gatcgagacg gaaaaggact atccgttgca gatgctatgc
    13921 ctggaggtaa gcagcgcttt gcgattatcg gcagtgaaga atttgactgg acgattgatc
    13981 aagaaaaata tatttacccg aatcatcgag gaatcgatca ttacgatcgt ttcaaagaag
    14041 atcttgcctt gtttgcggaa atgggattca aatgttatcg tttttcgatt gcctggacac
    14101 gaatttttcc aaatggagac gaaggtactc ctaacgaagc gggattagaa ttttatgatc
    14161 agctgattga tgagtgctta aaatatgata ttgaacctgt gatcacgatt tctcattacg
    14221 aaatgcctct tcatttggca aaagaatacg gtggctggaa aaatcgaaaa ctgatcgact
    14281 tctatgaacg atttgctcag acagtattag agagatacag cagtaaagtc aaatattgga
    14341 tgactttcaa tgaaatcaat tcggcctttc actttccagc tttaagtcaa ggattagtaa
    14401 aaagcaatgg agcaggagag taccaaaata ttttccaagc atggcataat caattcgttg
    14461 caagtagcaa agccgtaaaa atcggccacg aattgcgttc ggatatccaa atcggctgca
    14521 tgattatcta tgcaacaact tacagcatcg atgccaatcc agtcaatcag gcagcaacaa
    14581 tgatccaaaa ccaagaattc aattttttct gcacggatgt ccaagtacgt ggagaatacc
    14641 ctgcatacac agcgcgtacc cataaaaaat atggagtcga tccggaaaaa ttagagcaaa
    14701 cggaaaaaga cttccagttg ctaaaagaat atccagtgga ctacatcgga tttagttatt
    14761 atatgtctac ggcaatcaat gagactgatc cacaagcagc aacttcagaa gggaacttgc
    14821 tgggaggagt aaaaaatccg tttcttgagg caagtgaatg gggctggcag atcgatccag
    14881 aagggctgag aatcgcattg aatgaattat acaatcgtta ccagaaacca ttattcattg
    14941 ttgaaaacgg tcttggagcc attgatcata tggaagagga tggcacgatt attgatgatt
    15001 atcggatcga ttatttacgt cgtcatatcg aagcgatggc taatgcagta gcagacggtg
    15061 tagatttgat gggctatacg ccttggggat gtatcgatct tgtcagtgca tcaacaggag
    15121 aaatgagtaa acgttacggc tttatctatg tggatctaga cgatgaagga aatgggacac
    15181 tgaatcgatc aaagaaaaaa tcttttgatt ggtataaaga agtaattgcc tcaaatggcg
    15241 taaacttgta attaaagcag ctgaatgaaa aagaacacac ttctcacatg tgatttgtgt
    15301 tcttttttta ttttttatgt gctattcttt tagtaatagt aaagcaaagg gtgacgttat
    15361 gaaaaggaca cgtgtactat acttggaagt cgcagataaa attaaagaag atattttctc
    15421 aggaaaatat ccagtcggtt cgatgttgcc gacagaaagt gaattagaag aactgtttca
    15481 agtcagcaaa atcactgtcc gaaaggcaat cgaactgctg gcatcagatg aatatgtcgc
    15541 aaagaaaagt ggtcgtggga cgacggttct aagcaatcgt ccttacaatc gattatccaa
    15601 agcggcaagc tttacgcaaa tcttggagaa ctcccgatat gaagtccgga aagaaacgct
    15661 ggatttgcaa ttggtccata taaagaagga agatcctttg ttttcctttt tcggtgagca
    15721 agctgtttgt tttaagcgct tatattattt gaacaatcag ccttatattt attttgttca
    15781 ttatcttcca caagaaatga tgcaattcag cagagaagaa tttggcaaag aatctttgta
    15841 tcgtttgctg acgaaaaaag gatatactat tgactcattt tctgatgatt tctcggcagt
    15901 atttttaaca gaagaagaac aaggaattct gaaaacagac gcacagatcg gcatcaaacg
    15961 gatacggaaa tcgtctacgc aaattgggga aatgattgaa tattcggagg cggtctacca
    16021 tacggagctg catccttacc atattgatta tgaaacataa ataaaaaaag aggttgtgaa
    16081 aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc
    16141 gtttggttag gcttattacc gcagatacac ggctgtttct ggattaccat ttattctgga
    16201 taagggggtg tggcacgatt ttcagaagga atctatgaac caatcactgt attgatttta
    16261 gcttcgatgg taaccccatt cggcttgacg atcgcttatt tcttagggaa aatcattcgt
    16321 aaaaatatac tcaatcgtca agaaatcgat acattgaaga ccgcttttcc tatggggatc
    16381 tgtcagatca cagagggatg tttccctatc gtattgaacg acttggcacg caatgtcata
    16441 gcaacgggag tcggtgggac agtaggtggc ggattaagca tgttttgggg cgcagatagc
    16501 cgtattccgg catcgggaat gtttgcagtc gctacgatga ccagaccatg ggcattcatt
    16561 ggtgcattgt tagctggttc ttttgtcaca ggaataacca ttttattatt gaaaaaacca
    16621 gtagatccga atgcagaaat catccaagaa gaaaaagagg aagaagacat atcttgggat
    16681 gatttgacaa ttagttagga gaggcagaat ggattacaag aaaaaagtac aatagatgta
    16741 ccttgagaca ggaatggtat gaaagaatca gagttagaaa gaatcgaata tacagatttt
    16801 ggtttaggag cgatcgaaac tgtccagagc atcggcaccc tccttgaaat gacgatgaag
    16861 gaaaaatgag aacctttcgt tgtcgaaagg gtctggtata ccttatgtcg aaggaaagga
    16921 aacaaaaaaa ctttcctgta ctattccaaa aaggaaagaa agttattata cagtaaaaaa
    16981 agaaattatt cttcatgcta gagatcagta cacgttcgag cccaatatca aacactagtt
    17041 ccaggcaggc gcaacaggtg cggttatcac tgaattttct tccagcagtg acgattcaag
    17101 tgatattttt acaaatccag ctatttatcg ttaacaaaaa gatttttagg agaagctgat
    17161 gaacaaaaat gatcaaattt tgatcgttta gttcgtcggc ttttttattt agagaaacat
    17221 catttatcag tgaaacataa cacgagaaca aaatgatttt ttcgttaata aaagaaacaa
    17281 gtatttgttg tttaaaataa tacacgttat tggcatatag gacgaaatcc cagtatcttc
    17341 tataagtaag tcatttcata tcgatgaaaa cacgttattt atttttgttt tatctataaa
    17401 atggct
//
LOCUS       DAZKBH010000051        17301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000051 DAZKBH010000000
VERSION     DAZKBH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.051"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..354)
                     /locus_tag="ACG8OO_001620"
     CDS             complement(<1..354)
                     /locus_tag="ACG8OO_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045501.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFY"
     gene            621..2321
                     /locus_tag="ACG8OO_001621"
     CDS             621..2321
                     /locus_tag="ACG8OO_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9045502.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2291..2509
                     /locus_tag="ACG8OO_001622"
     CDS             2291..2509
                     /locus_tag="ACG8OO_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFP9045503.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2529..3419
                     /locus_tag="ACG8OO_001623"
     CDS             2529..3419
                     /locus_tag="ACG8OO_001623"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9045504.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3437..3721
                     /locus_tag="ACG8OO_001624"
     CDS             3437..3721
                     /locus_tag="ACG8OO_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045505.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            4208..4822
                     /gene="gmk"
                     /locus_tag="ACG8OO_001625"
     CDS             4208..4822
                     /gene="gmk"
                     /locus_tag="ACG8OO_001625"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9045506.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4827..5132
                     /gene="rpoZ"
                     /locus_tag="ACG8OO_001626"
     CDS             4827..5132
                     /gene="rpoZ"
                     /locus_tag="ACG8OO_001626"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9045507.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            5385..7793
                     /gene="priA"
                     /locus_tag="ACG8OO_001627"
     CDS             5385..7793
                     /gene="priA"
                     /locus_tag="ACG8OO_001627"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9045508.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7808..8299
                     /gene="def"
                     /locus_tag="ACG8OO_001628"
     CDS             7808..8299
                     /gene="def"
                     /locus_tag="ACG8OO_001628"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9045509.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            8292..9230
                     /gene="fmt"
                     /locus_tag="ACG8OO_001629"
     CDS             8292..9230
                     /gene="fmt"
                     /locus_tag="ACG8OO_001629"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9045510.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            9230..10588
                     /gene="rsmB"
                     /locus_tag="ACG8OO_001630"
     CDS             9230..10588
                     /gene="rsmB"
                     /locus_tag="ACG8OO_001630"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9045511.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10601..11341
                     /locus_tag="ACG8OO_001631"
     CDS             10601..11341
                     /locus_tag="ACG8OO_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFP9045512.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            11338..13407
                     /gene="pknB"
                     /locus_tag="ACG8OO_001632"
     CDS             11338..13407
                     /gene="pknB"
                     /locus_tag="ACG8OO_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFP9045513.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13572..14471
                     /gene="rsgA"
                     /locus_tag="ACG8OO_001633"
     CDS             13572..14471
                     /gene="rsgA"
                     /locus_tag="ACG8OO_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFP9045514.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            14484..15134
                     /gene="rpe"
                     /locus_tag="ACG8OO_001634"
     CDS             14484..15134
                     /gene="rpe"
                     /locus_tag="ACG8OO_001634"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9045515.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            15135..15773
                     /locus_tag="ACG8OO_001635"
     CDS             15135..15773
                     /locus_tag="ACG8OO_001635"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFP9045516.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15928..16782
                     /gene="mreC"
                     /locus_tag="ACG8OO_001636"
     CDS             15928..16782
                     /gene="mreC"
                     /locus_tag="ACG8OO_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9045517.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16786..17295
                     /gene="mreD"
                     /locus_tag="ACG8OO_001637"
     CDS             16786..17295
                     /gene="mreD"
                     /locus_tag="ACG8OO_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9045518.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
BASE COUNT         5794 a         3120 c         3757 g         4630 t
ORIGIN      
        1 ataaaaaaac ggcgaatgaa gatgtgtttt catatgacca aaatataata attctgaaat
       61 ttctaaaggt gtcaattctt tcagcatcgt taaatcagta aagtcgatcc acttcaattc
      121 atcgaatcca cgcctgttac gagaagagga aaagaaacgc tgaatatttt ctgtaccacg
      181 gattactttc agccctgtat gggattcata ttctccagca gaagcagatg gatcaagcaa
      241 gagcaagttt tgaggaaaat gcacgatact gtttgcaaag tcagtagcag tcagcccttt
      301 tgttaaaaca gcattacttg tggtatcaat atgtacataa actgactcag tcatcatgtt
      361 cctcctctta tttcctctta atctctttta ttttacaaca tttgcattaa aaaagcttgt
      421 ttcttttatt ttaaaattcc attacaaaat cacgttttca ttgaaaacac tctcattttc
      481 aattaatttt tgtgattttt gtattttttt atttaaagga ggttttatga attgttttct
      541 tatggaatct acaaagtcga atacgagact catgaccttt ttatgacgta ctggtatact
      601 tgaagaaaaa ggagagcgcg atggagataa aaaaagttta cttttcacag aaaatgtcgc
      661 tgtattcgcc tatccagcaa gcaaaacaac cccttccatt ttttcaaagt catcaagaaa
      721 gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc ttttaaagca gtatcgaaaa
      781 aatataccgt ctattttact attgctaaaa gtagaggaga tgttattttt aataatctgt
      841 tgattgaagg tgaaactttt gaatggaaaa aattttttcg gtatatggaa gcttgcgctc
      901 gtttttttat taaagaaaac tgttgttttt tatttcaatc tcctctatca gaagaatggt
      961 tgcgtatttt tcataaaaac ggctatcaag gaaccacaca attaaataaa aagctggtct
     1021 atcatacagc acttgtttta ggtggaggag gagcgcacgg tgcttatcag attggtgtgt
     1081 ggcaggcgct gaaggaacat gatatcaact ttgagattat cacaggtacc tcggtaggtg
     1141 cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa agctgtgaat ttatggaaaa
     1201 agctgtcgac aagacaagtg cttgcgttac cagaaatggc tgcagttgaa gaccttcgtg
     1261 aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc actaattgaa ggcggggttt
     1321 cttctgctcc attggaaaag atggtcaaag acaatttgga tttgtcctta ctcaaacata
     1381 atcccaaaat acgtttatat acggtgtcta ctaaactgcc agaactagcg gaagtagtga
     1441 ctgatatcca acagacaaaa acagaagaaa taccagattg gatactcgcc tctgcttcat
     1501 tttatcccgc tatggcttat cgaaagatcg gaaaaaataa atatgcagac ggtgggtatc
     1561 gcaacaaaat acctattgat attgcgatta acaaaggagc aacagaggca tttgtcgtag
     1621 atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc agacattttt atccattgga
     1681 aatgtcaaac attgtggaca ctaggcagct ttctgctttt tgatagccaa aggaaccaat
     1741 taaatttaca attaggctat ctggaaatga aaaaacgatt aggctattac tatgggaact
     1801 ggtatacatt tcattctgtt aaacctgcaa gaacatactg gcgaggattt ttgtcttact
     1861 taacaaaaga aattcagtta gaattatctt ttttcaaaag cataaaattt tggcaaaaac
     1921 tgcgtaattt atataagaac agggtcgtac cagagacatg cggattggcg atgctagaac
     1981 ttttagcaaa aaaacatttt ttactgccca atgaaatcta tcaagtagac atgatgattc
     2041 aaatgatctg ccaacaagat ataaaaagta tgccggatga tttattggca caaattggtc
     2101 aattaagtac tgaagaatgg cgaaggtatc gaaaatacca gcaaaaaata caaaatgagc
     2161 gcacgaaaac tgtttatttt gattatctgc tacaaggaaa acgcaaggat cgtttgcagc
     2221 atgaattgct taagcaacca gttgacacat tactgatcct atatttatat tatctcaagg
     2281 aggaacaacc atggcacaag aattttcata tgaaatcgta gaagaaatcg ctatattgtc
     2341 agaaaacaac aaggggtggc gtaaagaact gaacctagtc agctggaatg gtcgcccgcc
     2401 aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa atggggaaag gcctgacatt
     2461 gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa aatatgtaat gaggaaggac
     2521 cctgtcgaat gaaaagtatg actggttttg gcaaagccat ccgcgaaaca aaagcgtatc
     2581 agttagatat tgaaataaaa agtgtcaacc agcgttttct agatgttcaa atacgaagtc
     2641 ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct tattaaacag cagttaagcc
     2701 gcggtcgaat cgaagtattt gtgaatctag cttatattgg caaaaacgaa aaacagatcg
     2761 tcatcaactg ggaattgatt gattcactta tcactcaatt gacagacgga attcaacaac
     2821 gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc ggaacgcttc gtgctggacg
     2881 aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat ggaagaattg gcacagttag
     2941 ctctagatac attgaaaagc gcacttgatt ctattgaggc gagtcgagaa aaagaaggtc
     3001 aagctttagc tttagtgttg aaaaagaata ctgaagaatt caaagaagta ctaaaaaaac
     3061 tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat aaggtatcaa aaaaagctgg
     3121 aggaatttct tggagcgaaa gtcgatgagc aacggttgtt gacggagcta gccatcttgt
     3181 tggaacgcgg agatattcac gaggaactgg atcgtctggc gatccatgta caaaaactag
     3241 atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt agatttcctg atccaagaaa
     3301 tgaatcggga aatcaacacg atcggttcca aatcaaatgc aatcgaaatt aaaaatcaag
     3361 tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca agtacaaaat attgaatgac
     3421 gaaagaggta gagataatga aatattatga cgtaacgttc cacgaattaa gtggaaaaac
     3481 tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt gacgtatgga aagatgcctg
     3541 tgcagactat aatgagaacg aactgtttat tttgatcaac gacggtgctt acgtgacgat
     3601 gaatcggaaa tttattgttc gaattgacac agaagaggta gaagatccga cagaaaaagc
     3661 acgttctcga aaagatgaga tcatgggtgt agtgaatact ttgtccaata tgggctttta
     3721 gcaaggatgc tgaacaaaga aatacaataa ataaacagaa ttggatttct tcgcttagct
     3781 tctttgctct aggaatacat ttaaaggttg tgacaaaatt cgtgtcacaa tcttttttta
     3841 ttttgaataa gtgatgttca aaaaccattt tctcggtatt aagccaaatt tatgcaaaaa
     3901 tgtgagaaac tattttcaca aaatttttct tcatactcga aactaatcgg cttcttcacg
     3961 acctcttata cacggcgttc aaaagcttgc atctgcgata ataaccccaa ccaaacgaaa
     4021 aacatgagaa gctgtttatg tttggctgtt cttattactt gatgcaggac agctttttta
     4081 cgacctcttg gtacagggta gcttttttac caatttctag ctataattaa agataacctt
     4141 gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac aaaaagacta gttgaaagga
     4201 cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct ggtccatccg gtgtaggaaa
     4261 aggcacagta cgaaaagcaa tttttgagag tgaggataat gattttcagt attcgatctc
     4321 aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc gttgattatt tcttccgaag
     4381 caaagaagag tttgaatcaa tgatcgaagc tggagagatg ctggaatacg cagaatatgt
     4441 cgggaattat tatggaactc ctttgtcata tgtcaacaag acattggatg aaggaaaaga
     4501 tgttttcttg gagattgaag tccaaggagc aaaacaagtc aaagaaaaag tgcccgatgg
     4561 tgtattcatc tttttgactc cgccagattt agcggaatta aaatcacgga tcgtaggccg
     4621 aggaacagat gcagatgaag taatcgaaaa acgaatgaaa gttgcaaaag aagaaatcga
     4681 aatgatggct ctttacgact atgctgttgt aaatgatgaa gtgccgctag cagttgaacg
     4741 aatcaaaaac attattgcca gtgaacattt tcgagtagat cgcgtaatcg gtaaatatat
     4801 taaaatgtta aaggagatgt agtcttatga tgttaaaacc ttcaattgac tcactattag
     4861 atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag taaacgcgcg catgaattgg
     4921 atgcaagtgc acagcctact ctagattctt ttgattcagt aaaaagcgtc ggtcaagctt
     4981 tagaagaaat cgatgcagga aatgtagtca atgatccaca tcctgaatta aaacgtgaac
     5041 gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga gcgtgaacaa cacgaattgg
     5101 aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa aatagagcga acatctaaaa
     5161 agaaaagcaa acccagctgt ttccttttca aacattcgcc acttattgtc tggtagaaaa
     5221 ggctgtgtct gaactttttg acacagttct tttttttctt atctgctgtt aattttttgt
     5281 taggatttgg cagaagataa gacaaatcca tcgatctcta gtaaaatagg caaggaactt
     5341 tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg aaccatgaca ttaacagctg
     5401 aagtcattgt ggacgtacct actatgcaga ccgatcagcc attcacttat ttggttccct
     5461 ctgaaatgga aacagctttg caagtcggga tgcgagtaga agtccctttt ggtaatggca
     5521 atcgtcatgt ccaaggattt gttatggcta tccaaaaatc agaagaatca gctaatccat
     5581 ctttgaaggc agttattcgt ttgctagacc ttgctccggt ggtcaatgaa gaattacttt
     5641 cattagctga ttatatgaaa aaaattactt acgcattcaa aatcacttgt ctacaaacga
     5701 tgcttccgag tgtgatgaaa gcagaatatg ataaattgat ctatcctttg gcagacacac
     5761 cagaagttca ggtattgttt gatcagcgag aagtgatttc ctggaaggaa gcagaagaag
     5821 aaggcagcct ttctcaattg attcgctggc gccaagagca attagtagat atcaaatatg
     5881 aagtccatac acgaaataaa gtaaaaacga ttcgtttggt ccgttctttg ttaactgaaa
     5941 agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc caagaaacaa aaagaacttc
     6001 ttttgtgttt atctgaactg agccaagaag aaccaattgc ttatttcaaa aataaaggaa
     6061 tcagtactgc tgtcttgaat caaggaaaag aaaagggctg gctggagttt atagaatcag
     6121 aacgttatcg cgacccttat aaagaccgag tatttgacca aacgacggca ttggaactga
     6181 atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc aggtcagcag caaaaagatc
     6241 aggtcttttt gctggaggga atcactggca gcgggaaaac agaagtatat cttcaggcaa
     6301 tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt agtgcctgaa atcgcattga
     6361 ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga atcagttgcg gtcttacata
     6421 gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa gattgagcgg gaagaagcgc
     6481 aagtagtagt aggcgcacga tctgctattt ttgccccgct gaaaaatatc gggctgatca
     6541 ttatcgatga agaacatgaa tcaagttaca aacaagatga gacaccgcgt tatcatgcga
     6601 gagatctagc aatttggcgc agtaaatatc atcattgccc aatagttcta ggaagcgcga
     6661 cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt ttatcagtta ttgcagttaa
     6721 accaccgagc caaagcagct gcacagcttc cagctattga gatcgtagat atgcgtgaag
     6781 agtttcagaa tcatcggacc tctacttttt ctgctaattt gcaggaaaaa atacaaaatc
     6841 gattagacaa aaaagaacaa actgtcctac tgctcaatcg tcgaggatat tcgtcttttg
     6901 tgatgtgtag ggactgtgga tttgttcttc catgtcctaa ttgcgatatc tctcttacac
     6961 tgcatatgga tacacgttca atgcgctgcc attattgcgg acatgaagaa cctattccca
     7021 atcgctgtcc taattgtggc ggcaataaaa tccgctacta tggaacaggt actcaaaaag
     7081 tagaagagga actccgagaa ctttatccgc aagctaggat tttgcgtatg gatgtagata
     7141 ccactcgaag aaaaggggca cacgaacaga ttttacaaaa attcggtgca aaagaagcag
     7201 atatattatt agggacacaa atgattgcta aaggtttgga ctttccagaa gtgacattag
     7261 ttggggtgtt gaatgctgat acctccctga atttaccgga ttttcgttct agtgagcata
     7321 cattccaatt gcttacccaa gtttcaggac gagcagggcg agcggaaaaa gcaggcgaag
     7381 tcgtgatcca aacattcaat cctcaacatt acgccataga attggctaaa aaacaaaatt
     7441 atgagcagtt ttatcagcag gaaatgcatg tacggcatcg tggaggctat cctccttatt
     7501 actttactgt gaaaataacc gccagccatc ctgaagaaca agtagcggca aagaaaatct
     7561 ttcagattgc caatcagcta aaagaagtgt taagccctca aagcttgcta ttagggccaa
     7621 cgcccagcat gattttgaga gtgaaaaacc gatattatta tcaactaatc atcaaatata
     7681 aacatgaacc gaatcttcct catgttttgg atgaaatttt gaatggaagc cagaaagaac
     7741 agcgacaagg tctttttgtg gcaattgaca acgaaccatt aaactttatt taagaaaggg
     7801 ccaaattatg cgctatccta tattgatcca ccctaatgat aaattaaaaa gaactgctca
     7861 gccaatcgat gtaatcacgg acgagacaat cgctttatta gacaatttat atgagacaat
     7921 gattgcgaat gacggaatag ggatcgctgc tcctcaagta ggacaaaaca agagaattgc
     7981 tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt aacccagaaa tcatcgaagc
     8041 aaaaggtgaa agccttgacg tagaaggttg tctcagtatt ccgcatgtct atggaacggt
     8101 caagcgagca gatgaagtga ccgtgcgtta ttatgatcga gacggagaag agatagaagt
     8161 gacggctttt ggctatttag cccgtgcctt ccagcatgaa atcgatcact tagacggtat
     8221 cttgttcatt gagaaaatga tacagcagat cccggaagaa gaacttgaag aatatatgga
     8281 ggaacatctt gatgactaag ctagtattta tgggcacacc tgctttttct gtgccgattc
     8341 ttgaagggct gatagaatca ggttacgaga tccaggcagt ggtaacacag cccgaccgtc
     8401 cagttgggcg gaaacgagta attacgccga caccggtaaa aaaagcggct ctgaagcacg
     8461 gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc agaaatggaa gaaatcattg
     8521 aattggtgcc tgatgtgatc gtcactgcgg catttggcca attcttgcct gaaaagttat
     8581 tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc tttacttcct aaatatcggg
     8641 gaggtgcgcc tgttcattat gccatcatga atggggagaa agaaactggt gtaacgatca
     8701 tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc gcaagaaagc ataccaatca
     8761 ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag ccttttagga agaaagctat
     8821 tgctagaaac attgcccaat attttggatg gacaaaaacc agtacctcaa gacgagtcag
     8881 aagcaacttt ttcgccaaac atcacacggg aacaagaagc aatcgattgg cataaaactg
     8941 cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg gccaattgca ttcacgacct
     9001 atgagggcat acgctggaaa ttattatcgg ttgaagtatt ggcagaaact acagacaaag
     9061 cacctggtac gatcatccgg aaagacaaaa aaaatctatg gattgcttgc ggtaaacaga
     9121 ccgtcttagc catcaatgaa ttacagccag ctggtaaagg aaaacaggcc gtacatgatt
     9181 tccttaacgg aagcggtcag caagttcaga ttggtcagca ggtgaaataa tgaaaaaaat
     9241 ccctaaaaaa atgaaaaaat cagtacgata tatggcatta ctgacattag agcgagtgaa
     9301 caagggtggc gcctactcga atcttttgtt gaatgagatg atcaaacaaa gcgagctaaa
     9361 tcaaaaagat gtgggtttat ttacagaatt gatctatggt acgattagcc atcagcgact
     9421 attggaattt tacgttgcac cattgatcgc taaagcaaaa aaagtggatg actgggtcaa
     9481 gatgcttctt tatctttctg tataccaatt agaattttta gataaagtcc ctgcacatgc
     9541 tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga aatcctggga cagggaaatt
     9601 tgtaaatggt gtcttgagga attatcagcg tcaaggagca ccagatctgc aaggcattgc
     9661 tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct ctatggatga cgaaacgact
     9721 ggtggatcaa atcggatacg aagaaactga aaagctcggt ttatcgctat atcagcctag
     9781 ccatgctagt gcccgagtag atacacgccg actttccaga gatcaggcca tcactgtttt
     9841 gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt ccatacggta tagtagcgaa
     9901 aaaagggcat ttagcttcta gctcattgtt ccacgatggc gtcatgacga tccaagatga
     9961 aagttcgatg cttgtagcgc cgtctatgca aatcgagcct gaacatcatg tcttagatgc
    10021 ctgtgcagca cccggaggta agacgacgca tattgcttct tttttagatc cgtcatgcgg
    10081 tggagaagtg gtcgctttag atgtacacga acacaaagtc aaactcattg aagataatgc
    10141 agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag atggatgcta gagaagtcag
    10201 taaacagttc gaagcagagc agtttgatcg gattttagta gacgcgcctt gttcaggtct
    10261 tgggctgctt cggcgaaaac cagatattcg ttataaaaaa cagccggatg aattagcgaa
    10321 tttaccaaaa atccaattgg aaattttaga aagctgcgca ccaactttaa agtcttccgg
    10381 tattctgact tatagcactt gtacgatctt aaaagaagaa aaccaagacg ttgtttctgc
    10441 tttcttggaa agacaccctg attttgagag gattgatgtt ttgaccaatc ctatgctaca
    10501 atcatcaatc gaagagaaaa tgttgacttt gtatccgcat cagttttaca cagatggatt
    10561 tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa atgcagattg aatatcaatc
    10621 agatgtagga agacgacgta acacaaatca agactatgcc agtgtattta ccagccgttc
    10681 aggtctcaag cttgctgtat tagcagacgg gatgggtggt catcgtgccg gagacattgc
    10741 cagtcagatg gcggtaacga atctaggcga agcttgggaa aaacaagaag ctgttgacga
    10801 tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa gaaaatatgc tgatttatca
    10861 gcgtggacaa gaacagccgg aatataacgg tatgggaaca acaatcgtag cggccgctct
    10921 gtcagatgac tggttcacga ttgccaatgt tggggatagc agagcttatt tgattcgtga
    10981 agataaaatc atgcaattga cggaggatca ttctttagtc aatgaactag tcaaatcagg
    11041 agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa aatattttaa cacgttctgt
    11101 cggcatgcct ggaacagtcg aagtagatgt ttctactcat ctttggcaac taaaagatcg
    11161 actgctgtta tgttcagatg gcttgacgaa catgctgtct gaagaagaaa taggaacgat
    11221 catcaatcaa gaaggcccat tgtctcataa agtaactgag ctgattgatc gtgccaatga
    11281 agcaggcgga gcggataata tcaccgttct tcttatcgaa tataaggagg acagtgtatg
    11341 atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca ctggaaatat cggtagcggt
    11401 ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg atcgtgatgt agcagttaaa
    11461 gtcttacgat ttgactttca aaatgaccag actgcgattc gacgatttca gagagaagct
    11521 ttagcagcaa cagaactcgt tcatccaaac attgttagtg tttacgatgt tggtgaagaa
    11581 gacaacatgc agtatctggt gatggaatac gtcaaaggaa tggacttgaa acgatatatc
    11641 caaacacatt atcctgttcc ttatgaaaca gcagtaaata ttatgcagca gatcctttct
    11701 gctatttctt tagctcataa ccatcaaatc atccaccgag atttgaaacc ccaaaacgta
    11761 ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg ggattgctat cgctttatct
    11821 gaaacatcga tcacgcagac gaatacgatg ctaggatctg ttcattatct gtcccctgag
    11881 caggccagag gaagtatggc aacgaagcag tcagatattt atgctttagg gatcatcctt
    11941 tatgagatgt tgacgggtag cgtaccgttt gatggtgaat ctgctgtaac aattgcttta
    12001 aaacattttc aagatgatct tccatctata aaagcacttg atccaaatgt gccacaagca
    12061 ctggaaaacg tgattttacg ggcaacggct aaagaacctg cagaccgtta taagtcagcc
    12121 gaggaaatgt ctgatgatct ttctacagta ttatcaccag ctcgtgcaaa cgaagaaaaa
    12181 tggcagcctc atgtgatgga taatgaaaca aaagttatca caccccttac ggaagatgca
    12241 ccaatgcctg attcattcaa atctatgcct ctgccaaaag acaagccaga aaacgaaacc
    12301 ttaccagagg aggaagaagt tctgcctgat cctaaaaaga aaggacagaa aaaatggtgg
    12361 ctgatcctat tagctattct tgctgtttta ggaatcggaa cagctgtatt ctttgcttct
    12421 ggcgggcgag gagaagttgt tgttcctgat gtttctgatc tttctgaagc ttctgctaga
    12481 gaaacactcg caaaagcgaa attagaagta gcaacaaaaa cagaagaaat tgcagatgac
    12541 aaaatcgaag aaggtaatgt agtgaagaca gacccggcag cgggaacgac agtcaagcaa
    12601 aaacgcgaag tcactctgta tattagtagc ggcacgaaaa aaatcaaatt agatgattac
    12661 agtggtaaaa gttatgaaga tgccgaagca agtttgaaag aattagggtt ttctgctgac
    12721 ttgattcaaa aagaagagga attcagttca gaggtagaaa gcggaatgat catttcgcaa
    12781 tctccatcag aagatacaga agtcgatcct aaaacagaca cgattacttt cacagtcagc
    12841 aaaggaccag aaacagtgac tttgagtagt catgtaggag aaaactacga aactgttcgt
    12901 aattggcttg ttacacaagg attcaatatc aataatatta gtgctactta tgactatagc
    12961 gacactgtac catcaggttc gatcatcagt caggatccag cctcgggtga agttgtagca
    13021 gaagaaactt acatcaattt tgtcgtaagc caaggaacag agcctaaatt gaatgatatc
    13081 tccggctata caaaaagcga agcacaaagt tatttagcta gtgtcggtgc agaatatatc
    13141 gggcatgaga cttccgaata ttcaaacacg gtggataaag ataaagtgat tcgtacagta
    13201 ccaggggcag gaacgacaat taccaaagga atggttgtga acgtaattta ctctaaagga
    13261 ccagatccag cgtcttctag ttctgaaaaa gactcttcag acagttctag cagctcaagt
    13321 agctccagta gcacaagcaa ctcttcaaaa gaagaatctt ccagctccac aagttcatca
    13381 gctagcacct cagaaagttc aaaataagtg ctgaataaaa tatatcagaa gggaatgcga
    13441 cattgatttg ttgcattctc ttttttgtgt ttctatctct tttgatcaat tttcaagcca
    13501 tatactttga gaagtttgca tggaataaca cctactttcc tcttcgtatg gtaaaataaa
    13561 caaaaacgga ggtggcactt atcaaaggac aaatcagaaa agcaatcagc ggtttttact
    13621 atgtatacgc aaacggagag acctatcaaa caagaggaag aggaaatttc cggaatcgga
    13681 aaatcacgcc gcttgtcgga gatcaggtga tctttgagag tgaaaatcaa acagacggct
    13741 atcttctgga aatcttgccg agaaaaaatc aattagttcg cccaccagtc acaaatgttg
    13801 atcttggaat cattgtcaca agtatcgtag agcctgcatt ttcttataat ctacttgacc
    13861 gctttctagt taccttggaa tacgagtcga tcgaaccgat tatttttttg acaaagaccg
    13921 acttgtcaga cgacccagaa aaaatcgaag agatcaagca aacctatact aaaataggct
    13981 atccggtgat tgtacccaat catacgggtg atacgacaga actgatccgt tacttcccag
    14041 aacggctatc ggtctttatg ggtcagtctg gagcaggaaa atcgacgcta ctgaatcaaa
    14101 tatcaccgga actgaattta gcaacaggag agatttctga tgcacttgga agaggacggc
    14161 atacaacacg tcatgtggaa ctgattccta tctatggcgg gttagtagca gatacaccag
    14221 gatttagctc gattgacttt ttgacaattg aagcaggaga actgccgaga caatttcctg
    14281 aatttgtaga agcttctgtc cattgcaaat ttagagagtg catgcatgct aaagaacctg
    14341 gctgcgaagt aaaacataga gtagaaacag gtggtatcgc acagacacgt tacgacaatt
    14401 atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat gtatgcaaaa aaagaaggta
    14461 aacgaaaata ggaggaaaca agaatgaaga tagcaccatc cattttgagt gcagattttg
    14521 ccaatttaca aagagacatt gagttagtgg aaaaaggagg cgcagattac atccacgtag
    14581 atgtaatgga tggccgattt gtcccaaata tcacattagg gccaaatatt gttcaggcaa
    14641 tccgtcctgt tactaaatta ccgttagacg tccatctgat gatcgttgat ccagaaaact
    14701 acattccagc atttgcaaaa gctggagcag atatcattac tgtccacgtc gaagcaactc
    14761 ctcatattca tcgggcactt caaatgacaa aaagcttagg agttaaagct ggagtagtta
    14821 tcaatccagg aacaccagtc tccatgatca aacatgtttt gcctttggct gatcaagtac
    14881 tagtcatgac tgtcaatcct gggttcggcg gacaaagctt tattgaagaa acagtagaaa
    14941 aaattacgga actcgctgat ctacgtagag agaacaattg gacttatgca atcgaagtag
    15001 atggcggaat cgttccagaa acagcaaaaa tctgtaaaga tgcaggggcg gatgtcttcg
    15061 ttgcaggatc atatatctat ggagccgaaa atccagtcgg acaaatcgaa cgattaaaag
    15121 aggcattaga ctaaatgaaa attttattag ctgctggagg taatccgcaa aactggccgc
    15181 agtctctgct gtccaaatac gatgcctatg tcgggataga ccgtggtgcg ctgtacctgc
    15241 ttcaagcagg ctacccagta gatctggcag tgggtgattt tgattcattg tcgaacgaag
    15301 agcagaagga agtatttgca aaagccaaag agtatacccg ttcccctgca gaaaaagacg
    15361 atacagatac acagttaggt atcttaaaaa catttgaacg ttacccgcaa gcggaagtca
    15421 ctttgattgg agctacgggc ggaagattgg atcatctttt agccaatctc tggttaggta
    15481 tggaacctcg cttcaaaccc tttgcccatc agcttattat cagagattgt caaaattatt
    15541 tgtcttatta tttgcctgga gagcatatag taagacagat accagatatg aagtatttag
    15601 cttattgctg cttgactccc gtctcgaatc ttacattaaa aggcagcaag tatgtattgg
    15661 aaaataaaga agtagaatat ccgacatctt atgcaagtaa cgaatttctt tcagataccg
    15721 cagaattttc tttttcagat ggtatgattg cagttattca aagcaaagat tgatccataa
    15781 acaaataaaa aaatttgtct ttcaggctaa aaatgacaga atctattatt tgctaaaaaa
    15841 aagcttaaga ggagcaactt accttttgtt tcacggcatt tagtgataaa attgaacaca
    15901 agagtctaat taattcgagg tgtctttgtg aagaagttca atcctaataa aaatattatt
    15961 attaccttgg ttattgttat catcgtggtg acgatactaa gtgtgaccgc tgcgcagcga
    16021 gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta atgacagtgt tgcaatggtt
    16081 gataaaacaa tctctgcccc agtacggtgg gtacgaaatg gagtagattc tgtccataat
    16141 cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa aaatcgatag ctatgatgac
    16201 gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag cgttgaaaaa agaacttgag
    16261 ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa atgtcattac tcgttcgcct
    16321 gacagttggc aggacatgct gatcgtagac aagggttcta aagacggtat cgaagcaaat
    16381 atggcagtca tgtcgcaaaa aggattgatt ggccgagtga tcgaggtcaa tacggcttcg
    16441 tctaaaatcg aattactgtc atcctctaat gaaagctcca atcattttcc agtacgggta
    16501 tcttcggcta atggcgaagc gtttggtttg cttaaaaact atgatgaaaa gctccatgcc
    16561 ttagtggtga cccaattaac tggtgatacg gatatcaaag aaggggatgt tgtccagaca
    16621 tccggtcttg gagggaattc tccagctaac ttgccgatcg gtacggttat taaaacgaaa
    16681 ccagatagtt atgggctgga tcgggaagtt tatgtgaaac cttatgcaga aatgtatgac
    16741 gtgtcagttg tgacgattgt ccaacggcta gcggaggatt agtccatgtt gaagaaagaa
    16801 acaatgaagt actatctgcc aatcgttttg ttctttttga tgttgataga tggtcattta
    16861 acaagaatgc taggggagtg gtcgaaaggc acctatatgt caaatgccca ctttctgata
    16921 ttggcattat tatgttgcag tatggcgttt gaaaaacgtt atttactgat taccacgatt
    16981 gttctcgggg ctatctatga cgcttactat attggcgtta tcggtatcta tgcagtagct
    17041 ctccctttaa ttgtatggtt gatgtatgta atgaaagacg ttatccatgt caacatcttt
    17101 actgaatttt tcagtatgat catctttgtc acgggttatg aattgtttac gatggtggtc
    17161 cagttgattt ttaaattagc agtagtaaat aacacgtatt ttattacaag gtttttagga
    17221 cctacactgc tgttgaacat gattatattt gtattattca tttttccctt taagaaatta
    17281 ttcagtgatg aataatttct t
//
LOCUS       DAZKBH010000052        17144 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000052 DAZKBH010000000
VERSION     DAZKBH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.052"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..357)
                     /locus_tag="ACG8OO_001638"
     CDS             complement(<1..357)
                     /locus_tag="ACG8OO_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFP9045519.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNL"
     gene            1051..1437
                     /locus_tag="ACG8OO_001639"
     CDS             1051..1437
                     /locus_tag="ACG8OO_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9045520.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1531..1722
                     /locus_tag="ACG8OO_001640"
     CDS             1531..1722
                     /locus_tag="ACG8OO_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045521.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2236..2568
                     /locus_tag="ACG8OO_001641"
     CDS             2236..2568
                     /locus_tag="ACG8OO_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9045522.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2478..2672
                     /locus_tag="ACG8OO_001642"
     CDS             2478..2672
                     /locus_tag="ACG8OO_001642"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9045523.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            2711..2857
                     /locus_tag="ACG8OO_001643"
     CDS             2711..2857
                     /locus_tag="ACG8OO_001643"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045524.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            3295..4635
                     /locus_tag="ACG8OO_001644"
                     /pseudo
     CDS             3295..4635
                     /locus_tag="ACG8OO_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4876..6492)
                     /locus_tag="ACG8OO_001645"
     CDS             complement(4876..6492)
                     /locus_tag="ACG8OO_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9045525.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6610..6828
                     /locus_tag="ACG8OO_001646"
     CDS             6610..6828
                     /locus_tag="ACG8OO_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045526.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            7023..7484
                     /locus_tag="ACG8OO_001647"
     CDS             7023..7484
                     /locus_tag="ACG8OO_001647"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9045527.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7677..7916)
                     /locus_tag="ACG8OO_001648"
     CDS             complement(7677..7916)
                     /locus_tag="ACG8OO_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045528.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7940..9463)
                     /locus_tag="ACG8OO_001649"
     CDS             complement(7940..9463)
                     /locus_tag="ACG8OO_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP9045529.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9481..9603)
                     /locus_tag="ACG8OO_001650"
     CDS             complement(9481..9603)
                     /locus_tag="ACG8OO_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP9045530.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9633..10616)
                     /locus_tag="ACG8OO_001651"
     CDS             complement(9633..10616)
                     /locus_tag="ACG8OO_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9045531.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10640..10789)
                     /gene="rpmG"
                     /locus_tag="ACG8OO_001652"
     CDS             complement(10640..10789)
                     /gene="rpmG"
                     /locus_tag="ACG8OO_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9045532.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10810..11187)
                     /locus_tag="ACG8OO_001653"
     CDS             complement(10810..11187)
                     /locus_tag="ACG8OO_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045533.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(11219..11398)
                     /gene="rpmF"
                     /locus_tag="ACG8OO_001654"
     CDS             complement(11219..11398)
                     /gene="rpmF"
                     /locus_tag="ACG8OO_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9045534.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11413..11682)
                     /gene="rpsN"
                     /locus_tag="ACG8OO_001655"
     CDS             complement(11413..11682)
                     /gene="rpsN"
                     /locus_tag="ACG8OO_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9045535.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(12205..13971)
                     /locus_tag="ACG8OO_001656"
     CDS             complement(12205..13971)
                     /locus_tag="ACG8OO_001656"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9045536.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13931..15684)
                     /locus_tag="ACG8OO_001657"
                     /pseudo
     CDS             complement(13931..15684)
                     /locus_tag="ACG8OO_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(15794..16363)
                     /locus_tag="ACG8OO_001658"
     CDS             complement(15794..16363)
                     /locus_tag="ACG8OO_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFP9045537.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(16381..>17144)
                     /locus_tag="ACG8OO_001659"
     CDS             complement(16381..>17144)
                     /locus_tag="ACG8OO_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9045538.1"
                     /translation="VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEE
                     ITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSP
                     FLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQ
                     RLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKT
                     CNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         5562 a         3243 c         2549 g         5790 t
ORIGIN      
        1 caaattttgc aatgaattat agagtcgttc tctatcagcc attgcaacat tcaaattgtt
       61 catttttagt ttaaaaaaaa ctttatcaat attatttgct ctaaatggac caaataagta
      121 atatctggtt ttaaacatgt atagaagaaa catctcatta aaatcaccag taaagaaatg
      181 gaaatcttta gtttttttaa tattatcagt tagtaacttt ttataatcat aaaaaaaata
      241 agtagttcta tctgagcgat actcttttat aatactgaat tcggaattaa aaaccacgat
      301 aggtaacttt aaacaaacat gaagagactt taataagact tctttatctg aaaacacttt
      361 tgtttctctc tttcctttct acatcattaa atagtatttt atacgttatc taaaatacta
      421 ttcctctgtc aaaataagct aacttacttc attataaaat atccacttat cgctttcatg
      481 tatattccac cgtaaaaagt acattttatc accgagaact catcattttt catcatgatg
      541 aatactattt acatatatct agttcaaaga caataattat tatgatccgt tattatctaa
      601 gcactataca gataatagga atttttcgtt tctaaatatt ccaatttggg aatccaataa
      661 aaattttcgt tcaaatacgt gtctattcga actcttttct agttgttaat tatgaagggg
      721 gaagaaataa aaaatacaat ctatacacga ataagaatat tatccaaaag gaggttttag
      781 aaaagattta ttatacgtaa aaagctcaag acattttctt agccataagg acatgtttga
      841 tatttaagaa gatatatgaa ttttcttttc taagtataaa gataactatc cattatcaac
      901 taaaaaaaca tttttgaaaa taaacatcag cagtcctatc aatgcatgtg agcttataga
      961 agttattcgc agcgcagcat aattgcatta gcttcttttc gtgttttttg cctcttttcc
     1021 ctttaaatct taatggagga ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc
     1081 cagttgattc gattctttga aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa
     1141 caagaagaaa ttcaaaaaca tgaacaactt gatcaactcc caccatattt gtttactaaa
     1201 gatactataa aatttaaaca accgattaat gttttaaatg tgactctcct tgttgaagat
     1261 ataaaattag caaaaatcct tcctttgttg aaagaacctg aacaaatttt tcttatcgaa
     1321 aaatttatct ttgataaaac tgataaagaa attggaaagg ttctggggat tacaagacag
     1381 ggtgcgacaa atttaaaaca tcgactttac aaaaaaattc aaaaaatgct ggtttaatat
     1441 ctgcataata actaattttg tatgcttctt tgctcgttct cctccttccc aaagaaatag
     1501 attataagtc catgggaaga aggtagattt atgaattctt taaaagaaga tttcatttta
     1561 gcaaaggctg gaaatgaaga agctgttgag gcaattctca aaagatttag ctctttgatt
     1621 cataagcaat cctggagaac tggaaaatat gatcaagatt gttatcaaga atgtatgctt
     1681 gcaatatatc tagctatctc gaaattcgaa attaaagaat aactcaacta agggagtctg
     1741 tgacataagt aaaaagatag tttcaaattt cgaacaataa atttgaaact atcttcttta
     1801 taattataaa gtatcgattt tacagcgttc tataatgatt catagaatct aaaacgaatg
     1861 attttaatta tcaacgtttt ttaattcgtt tttagcttaa aaataacttt tgtcacagtc
     1921 tcactcctaa aattacttta tttacgtatg aagtgggcag gaggtcgaat ccagaactgg
     1981 gctatggtac ttcaacgagg acttctcctc tagaattgaa aagcatgcga tttaccttcc
     2041 gtaacgacgg tagatttatt caactatgtg tccaatcaaa atttgtctga tttcaaaaat
     2101 agtcaagaga attttggttg ttaaaaactc ttatctatca acttccacag tttagttgac
     2161 aaaccctgat actaagttgg gctatgatac acttttcttg actatcagtt acgttaccgg
     2221 aaggaatgaa ctctattgag aaaaacatat gatcgtaaat ttaaactaaa gatttcacag
     2281 gatatccttg agaaaaaagt aactaccaag aaaattgctg aagaatacaa tatctctcgt
     2341 ccaacaattt cccgatgggt ttcagaatat cgtcgatacg ggaaaaatgc ttttgctgga
     2401 caaggaaaaa gattacctga taaagcagat ttctacattc ttgaacagga aaataaacga
     2461 ctaaaagagg aagacgagtg ttctacgaac acaaagatcg atacggatct ccagggatca
     2521 ctcaaaaact ttacgatgaa ggaatcgaaa tcaataaacg tgttgtagct gttcttatgc
     2581 gtgaaatgaa tttatgcaca agaggatttc atcgacgcaa atcttctcat ggtagagaaa
     2641 aaacaatcaa agaaatggta aaaaaatgtt gaatcgataa tttgaacaga accaaattga
     2701 tgctgtttgg gtgatggata ttacttacat tccatgtaat gacggtcgcc tctatttgtc
     2761 cacctatatt gacttagcaa ctcgtattct acgttactat atggtagatt cacacatgaa
     2821 aaaagagatt gtcgtccgtc cattacaaat ttattaagga gaattgccta cggtaattca
     2881 ttccgataga ggtagccagc atcggtcttt tatttatcaa gagctgttga acgaccacca
     2941 catcacacat tcagtgagcc aaactgggac tcctgttgac aatgcagtaa ttgaagcgtt
     3001 tcatcgatca atcaaatgag aattaatcga ctccaacaaa tataaaagta aaatagagat
     3061 gaaggtgtta atccaagatt atttagaaga ctactaccca aacaaaagaa tccatacaaa
     3121 atttatgatg actccttgac aatttgaagc gaagaaaata agggatctat aatatctagt
     3181 gttggtgacg ccttctgttg ccaatacttt ttcttttttc taataaacgc aaacagacac
     3241 cttaaaatcc tttagaataa agtcgacgaa aaccattcaa aggagtgttt caagatgtcc
     3301 tattcagagt ttaccaaaga gttactagat attctagacc taaatcttat ctttcatgaa
     3361 gatgcctttc gcaaagagcg aatcaatgac gaaacatgtt ttgtttttga tggcacattg
     3421 acctatcaac cggaggaatg ctttcatcgc cactatcaaa atgaacaaac gatcattaaa
     3481 tagggctgga aaaaagtatc catcttattg aatgatgtca gtaactacaa gaccattctg
     3541 cggattaaca aacagcgctt caaatgtaaa cactgtggaa agactttttt agcagaagat
     3601 tcagttagtg atcgtcgctg ctccattgct cgcagagtca aacaagccat tcttgagcta
     3661 ttaagcgaac ctctttctat gtctttgatc gcacgtatga agcacatttc tccgaccact
     3721 gtcattcgaa tacttcgcag ccttcggcct aaaaccattt ctttgagcca gctgttacca
     3781 gaagtggtgt gtttcgatga attcaaatca gtgaaaaatg tctcgggagc catgagcttt
     3841 gtcatgatgg acggcaagac ccatcaactg attgatattg tggaaaatcg gcaactcaat
     3901 cctttacgcg actatttctt gcgctaccct ataaaagtcc gtgaacaagt gcgtctagtg
     3961 gtttcagatt tctatacacc ctatcgcaca ttggtgaaag agtgttttcc gaatgcccga
     4021 atcgtcgccg atcgctttca tattagccaa cacattggtc gggcttttac caaccaccga
     4081 attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt cgaagcactt aaagaaatac
     4141 tggaaactac ttcaaaagaa tgcgtgggaa ttgaaaggtc agcatcgcta ctggcgtccg
     4201 tcatttcgag atcatctgac agaagcagag atagtcgatc ggctattatc ttatgatgat
     4261 tccttaaagc gaggctacga agtctaccag gattttcttt ccgttatcag aagacaagat
     4321 gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg agttgccaga gcactaccaa
     4381 ccagtgttca cgaccttcaa gaaataccaa acagaaatca aacgagcgtt gcgcgttccc
     4441 tattccaacg gccctataga atgtttgaat aaccatatca aagtactaaa acgaattgcc
     4501 tatggctata gaaactttca aaactaccga gaacgaatct ttttatatcg aggaaaatat
     4561 tttaagaaaa ctaagaatac tacccaactg actaaagcca gaacaacaac acggctagac
     4621 aagatagctg tctagccgtg tgagtatctc gattattcta ttgaatttct tcaccaacac
     4681 tcattgacga agagccaaaa taagtaatct aaccgactgt taactaaacc attgcaggtc
     4741 ccgttaaaga tacttgtgcc tgttgtcacc gcttcttttt cttgtccaca agcgcttaac
     4801 acacttaata aagcccctgc tattattaat aataatatct ttttcttatt aaaaacctcc
     4861 tcatctattt ccagattagt aaatgtagta atctgtgctt tcttttttct caatactaat
     4921 aactaactta tggggtaaac aaatactcgt ttgaccgggt tttgagatcc atccagttct
     4981 aaccgcgatt tgatcaggac tattatcttc tttaactcga attcttccat ctttaatctc
     5041 aacaatatta tattgtccct tcgcaggatg ataattcacg agtttatgat tgatcttatc
     5101 caaatcgaag cgatccattt ccttgccatc aattcgaacg atggcatatt tgatagcatc
     5161 ttcagttgtt tcattctcct tttctataga gaatacaaat aagggaataa aagaggtaat
     5221 aatcaaaatg ccaataatta tgacatcgcc tatcttgaca ccagcttttc ttaaaaattt
     5281 cattaacgat atctggctaa acatattaga ctttttccat ggaaatggtg aaatttttcg
     5341 gaaattatct gcattaagag aatcagttaa acaaccctca agttgttcct taatacattg
     5401 ttgatccatc ttcataccaa taaaaactag tttattaatg cgttcaccaa acaacggatg
     5461 ccaggcttcc ttcaattcgg gatttttttg tataatcatt tgacactcaa ttttaggtaa
     5521 actagctacc caataattta atgattcaat acttttatag ctacccgatg tttcaaagat
     5581 cgtcgaaaaa tcactatgtt gggcaaacca aagaaaccct tttgctcttg taatattgcc
     5641 aggaaaattt ttatccgtaa gatgggcaaa tttttgtgcg tcaaggagta accgatttcg
     5701 atagataaaa cttgagatac catattcttc tgtttcagga atatgatttt gatagccaag
     5761 ctctaactct ttgaaccagc ccactgaata ggaagtctca tctatattaa aaagatttgt
     5821 atttagaatt ttcgataagt ctacttcacc tttaattgtc ctaattattt tggcattgga
     5881 ctgtaactta gcaattagac cgttaatttt gtaaatcgtc ttggttgaca ctaagtcact
     5941 attatttaaa agtaatatgt tacaacactc gaattgatca attaacaagc cacgaatatc
     6001 ctgttcttct tcaactacat taccttcagc ttttctttcc tgaatctttt cacccgtttc
     6061 gaaattatcc caaaaacgtt ttacatccac tactataata atggcaccaa tctctaatcg
     6121 ttgggtcaaa tcaatgtcta aagggttttt agctaatata attgcttgaa taattggaat
     6181 tggtttgctg attcctgaag cttcaataac aatttgattc aagtcagaaa attgcactaa
     6241 ttgatctagt tcaacaatca aatcttcgcg taatgtacaa caaatgcatc cattcggcat
     6301 cgaaacaagt ttttctccag ttcttttaaa acctttcttg tcgactaata ccccatcaac
     6361 atttacttcc cctatgtcat ttacaatgac agcaatttcc tttccgtgat caccgtacag
     6421 aatatgattt agaagtatcg tttttcctga accaagatag ccagttaaga ttgtaatagg
     6481 tatttttttc atagggaacc tctttctata ccttgaattt acactttaag tgcaacacat
     6541 aaaaatgagt cataatcgat atccaactag tataagtttg tcgagaacac tcctagaagg
     6601 aaaataatta tgagccagta tcgtcatctt actatactcg aacgagaaag aatctttctg
     6661 cttcacgaag aaggattttc tattagtata attggcaacg acattcagca tagcccgttt
     6721 actattttac atgaactgag aagaaacaag aaaaaaacta ttcaccttcc aaagcggaaa
     6781 aaaattatgc ttatcgaaga aaaaaatgcg gacgttatca cattctaaat gatccgtgag
     6841 tttggtcaat tgttcgtcat ttatttgtcg attttcagtg gtcgccagag gaaatctcga
     6901 atcgtttgaa gcatgaacat atggactttt caattagttt tatcacgatc taacgtgcaa
     6961 tttgcaatga tcttttcgat acagaacccc tctcacacgg caataaaggc ctgatccgtt
     7021 cattgcacca tcgaggaaaa acaagacata caaaaaatca tgtcgagtct cgtggaaaga
     7081 tttgccttac tcattcgatc catggccgtc caaaatccgc agatgataga agtgattttg
     7141 gtcattggaa agccgatatt gtagctggca aaactggtgg tacttgttta gtgactttga
     7201 cagacagaaa atcatgcttc cttttagcag gaaagaaggt ccccagaaag aaagccgctt
     7261 atgtgagcga gaaaatgatt gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga
     7321 tcactccaga tagaggaaaa gaattctcca atcatttcgc agttacagaa gcattgaata
     7381 atatgccctt ttattttcca gatcctcatg cccttggcaa caatgaacaa attaaaatac
     7441 gaatagactc attcgagaat acttgccaaa atcgaaagag atagctacgg tagataactc
     7501 aacgataaat cagatgattg aaaaactgaa taacagacct agaaagtgtt tagattggag
     7561 aacaccttat gaagtcttct ttgataaagt gttgcgcttg atttgacaat tcaagacata
     7621 ataaaaagca gatcaaatcc aaataaggga aaaatgatct actctggtat tatcaattaa
     7681 ttggccaagc tattatgccg ttttgctaaa cgtctacttc ttccagtatt gaccttgata
     7741 aaacaacaat caggatcccg ttttaatact ctatgaactg tgtcaatact aacatgtaaa
     7801 gttgcagcaa tatcagctaa tgtactccca tttaatctca tttgctttgc aaatcgataa
     7861 cgccgataag catcaatatt cttggaatcc atagacaata atacttttct taccatgtat
     7921 ttcctccaaa aattgaattt taatgagcca tcatttcttg agcaatttca tgtttagaca
     7981 atgaagctgg atagaaagta ggccaattat gcaattcatt atagagtttt tcttgacttt
     8041 ctccaccaaa gaaaatatgg aaatgctctg ccttacttgg tgcaattcca tgatcactaa
     8101 attgaatata tttaaaacga ccagcacctg catcatcagt ttcaaaatta aaacgaacac
     8161 cacggtttcc tttttcataa ttcaaaattt tgtatccaac atatttgtat gtgtattgat
     8221 gatgttcacc gttgactaca aaatcaatgg tgttgtcttt aatgttgata gtatcaatat
     8281 ctgtcttata gccagtagta taataatctt tgtactcggc agctgtcatg tctttattga
     8341 tttttgcttt gtaatcgaaa acttcatcca aaattccatc ttttaacaac ggataaacag
     8401 attgccattc gcctgtataa tcactcaatt cgcgatcctt cacatcggca tctttaaagt
     8461 acccattagc aactgtcttt tcatccttcg cttctttttc tggaactacc tcttcaccag
     8521 caaccatagt tgttttttct aacgctttta gattgtcttc cattactgaa acataatctt
     8581 caccattatc catttgttct tttgtaagac tttcaagagg attcagtact tccaatttta
     8641 tcccagcttc gttggccaaa gtttttgcaa ttttatcatt agcattcttt tcaaagtaaa
     8701 tataattgat cttatttttt ttaacatact cttttaattc agctaatcga ccagaagatg
     8761 gctcttcttc aggacttagt ccagcaatag gaacttgttt caatccataa tccaaagcta
     8821 aatatccaaa ggctgcgtgt tgcgtcacaa aacttttttg tttcgcttct ttaagactag
     8881 tggtatagtc agcatccaac cgtttcagtt tagtcaaata cttttcagca tttgtctcaa
     8941 atacttttgc ttttttcgga tacaatttaa ctaactgatc cttgatattt gaaacttcct
     9001 taattgccat tttgggtgaa acccacgtat gaggatctag ttcatgatgg tgatcttctt
     9061 ctccgtgatc atgatcttct tcttcactac ctggcaataa aatcatgtct tttgttcctt
     9121 ctactacatt aggcccagct ttcttccaac tttctttagc ttttggtacc catgattcca
     9181 tgttttcgtt atgatataca aaaacatcag catcatgagc ttttgccata tccttagctg
     9241 agggctcata gtcatgaggt tctgatcccg ctggaaccat taaatccacg ttcgcttcat
     9301 cgccgacgat gtttttggtg aaatcataca ttggatagaa tgttgttaca atttctaatt
     9361 ttccatcttt atcattcgat acttttgcat cattagtgct ggagcaagct cccaataatc
     9421 ctagagctgc tatcccaata agtcctacac tgattttctt cattcttttt ccttccttaa
     9481 ttatgctaat ttcttcattt tatttcgttt atgttctttg ataattttta atgccctttt
     9541 tctagtttta atattcgggc ttttaagtcg tcgatatgca ctagatatat ccgttttctc
     9601 cattagaaat tacccccaat cactacttat cattatttgc tttccgccag taatttatca
     9661 aaactctgtt tgatcctttc ttcatccaac atttttccga ttagaataat agtggtttgg
     9721 tctttttgtc gagtggtgta ttgaaaatta acctgttcgt ttacctcttg catggcaact
     9781 ataaaatcct gctcttgtaa agcaatcaat cctttaaaac gatacaggct cccttggttg
     9841 gttaaaatca gccagtcaat ccattggagt aaaaaatctt tttttatgtc ttgatctgaa
     9901 gttaagagaa ttgatttaaa ctcatgtact tgatggtgtt gatgatagta tttgccatta
     9961 tagtgatttt ctgatgaatc actttcaaaa actaagggat gattaaattt tttcaaatta
    10021 taaaaatcct ttttttgaat cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg
    10081 ttaatacttt tgatttctct ctgtaatttt tttagtgcag ataaattcaa gttttgtttt
    10141 ctagacacaa aaatgcgatc tgccatgaca agttgcttaa gggcttgtgg ttctctttca
    10201 ataagttcaa aactgtcact atcaataaca gtcaataaac tatcgatata tactcttcct
    10261 cttagttgag gcgctgttaa aagtgtttga atgattggtt ggggatctgc tatacctgta
    10321 gtttcaatga tgatttgtga aattggttgt tgatgttctt ctattgtttc cagtaaagaa
    10381 atcaatattg ctaataaatc actgcgaaga ctgcaacaca tacatccatt atttagttgt
    10441 aacacccgtt cctcgctatg aactaataat ttgtgatcaa tcccaacttc accaaactca
    10501 ttttcaatta taataatttc ttccctgggg tagggtgaga cttgcaaggc ttgattgatt
    10561 agcgttgttt ttccagcccc taaaaaccca gaaacgattg taactggtat gcccattttg
    10621 tcaccttttt accacttagc tatttcactt ctttaaaaat tgcccgtctg cgcaattttg
    10681 gcgaatactt ttttaattct aaccgttcag ggttattccg tttatttttg ctggtgagat
    10741 acagacgttc tccagtttcg acacattcca aaataatatt ttgacgcata aatcttcctc
    10801 cttcaatact tatcgatctt caagatatgc ttgaatttgt tgtgcatctt caaaagttaa
    10861 ctcgccagat tccaattgtg aaactaaatt ttctttcata tcaacaggta tctcccaata
    10921 accatcgata cattgaaata actcctcgtc atactcttca aaaattggat agattcttaa
    10981 aaaatcatca aatgataagc cttggtcctc atagtaatct ttgactaatc cattttcaat
    11041 tgcctgttgg gtcggtaaac ccgtttcagt aataattcct ctctctacca tcgaacgata
    11101 aaaagtgcat ttatctagcg attgctgggt cgtttcaaga taatacttta atccttcttc
    11161 aactaaaggt gggtaaataa tttccatttt gattccctcc taaaagttat atattaaact
    11221 attttgaagc tcctttaatt actttctttc cattatagta cccttttaaa gatacatgat
    11281 ggctacgtct ataatcacca ttcgcttcgt caaatgaaat ttctggtttt actacagttt
    11341 gatgagttct tcttaaccgt ttcttcgcct tagatgtttt tcttgctggt actgccatga
    11401 tatttcctcc ttttaccaac ttgctttttt tactccagga atcaaccctt gatgggcaag
    11461 ttctctaaac ttaatacggg acataccgaa ctttcgcata taccctcttg gacgaccatc
    11521 cgtctgatca cgaagcctca gacgattcgg atttgaatct cttggcaatt tagcgagagc
    11581 ggtgcgatct cctgcggctt ttaattcctg acgtttatct gcatactttt caatcatcgc
    11641 catttgtttc tttgctttag caattttcga cttttttgcc atataccttt atactccttt
    11701 tattttgatt tagacacctc aaatcgtaat cgttacgttt tgacactgat actttaccaa
    11761 caagagttgc tcttgtcaac aataaataac atgaaataaa actcatatga atagataact
    11821 atttgaaaac gaatttatcg gtacatagag ataatcaaat catttaaaag aatatgcagt
    11881 ttaggctctg aaaaataaga attcactttt attagtccaa tcaaacacaa ataccgtttt
    11941 aagtagcttt tcctctactt aaaacggcaa tatgaaagaa actataactt tttattcaat
    12001 cttttatgtt taactggttt atcgccaatt ttttaaaact ttgattattc cttatattat
    12061 aagaatagac attaagtgaa ctaattcaat catactgatt ctttcgtgta ttcaaaatta
    12121 gatgctgttt aataaaatta ttctttttga aaaagaatta taactatatc ccaaaaattg
    12181 taggttaatt taaagcctat ttcatcatcg tgattgagct tctttttcat agacgaattt
    12241 ctcagatagt ttagttttct ttatgagatt tttgtatatt tctgaccttc gatacaactc
    12301 attatgagta ccttgatcaa ctagttttcc ttgatccatc actacaattt tatttacctt
    12361 ttcaatagac ttcaagcgat gcgatataat tagtactgtc ttatcttgta ttaaacgatt
    12421 taaactttcc tgaatacgtt tttcattttc cgcatctaaa ctggcagtaa tttcatctaa
    12481 aattaaaatt ggcgcatttt tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc
    12541 tccagaaagt gaacttccgt tttctccgac caatgtatga taaccgtcag gtagtttttc
    12601 aataaagtct tcgcagttag ctaaacgagc tgcttttttc acttcctcat ctgacgcttg
    12661 actatttcca atacgtatat tttccaaaat acttgtatta aacaaagtta cctcttgaaa
    12721 aacaatagcg attttgctaa acaaagaagc tggagataca cgtttaatat catacccatc
    12781 aattaaaata cagccttcat cataatcata cagtctagac actaatttca agatactagt
    12841 tttcccggaa ccactagcac ctactaacgc agtcacttct ccttgtttag ccgtaaaaga
    12901 gatatgatct aaaattgggg tattgttatc atatgagaaa gagacatctc gcaactcaat
    12961 gtcaaaagat ttcagaggac tatctgatcc ctcttggata ctggtttcct tcatagctct
    13021 aattcgctgt attttaggag ctaaataaaa tatttcaagc actgcttctt tcatagaatc
    13081 gaaggaatcc tttattttta ttgcggctag taagtaacca acgacgtaga gtatagtcac
    13141 ttcacccgtt aacagtaagt gaacacctac tagaatgact actgctaaac taacatatga
    13201 gaaaatagag gataaggcca tgactgaaaa agtacttaat tctaccttta aatgaatcct
    13261 ttcactctct tccatttttt tgaaaagtct gtcttgaact tttttagata gattaaagct
    13321 attaatctct tgctgcaatt ctatagtttc ttgaaattct tccgagtttt ctctcaaagt
    13381 atcgtaatat ttagtattgg ctttcgtttg ggatttcttt gataacaaga ttaagacaaa
    13441 actaaataac gttggcaaaa taacagctaa tcccattttg acattaccaa ccaaaagcat
    13501 cactgaaata aaagggaaaa acagagccat accgccggct ttaggtattg catgactcat
    13561 cgcatgctca ataccttcaa cgtcagacat gatagtttga gataaatctg ataaattatg
    13621 ttttgaaaaa taggataatg gtaaatttga caaatcgtct gcaatttgca ctcttaaatg
    13681 cgcactttct ttataagtac tattatataa gttttcatac tccctatcta acaaccaata
    13741 catgaaaagc aaagtaacaa acgatacggc aaaataaagc caacggggct ttgtattttc
    13801 taaaactaat tcgtctatca gcatcattat aatgaatgct ggtaccatat taatgcagta
    13861 tacaaaaaaa ctagaaatag aagctttagt taaagcttct gcgccctgat cacttaaagc
    13921 aaatttgttt ttataaaaac ttttcattgt caagcctcca atcatttgca ttctcataat
    13981 tagtgaccaa ttgtttatac acttgatgtt tagccattaa ttcattatga tttccacgtt
    14041 ctactatttt cccttctgac aagacaagaa tttcatctac agatttaatg ctggataatc
    14101 gatgagctat cataataact gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt
    14161 catactcatt atctggatca atagatgcac tggcttcatc cataataaca atctttgcat
    14221 cttttaaaat tgctctggca atagctatac gttgtttttc tcctcctgac agataaactc
    14281 ctttactacc aattaaggta tgctctcttt cgggaaattt ttctaaaatc gattcgcaac
    14341 cggctaatct tagcgcgtca aatactttac ttgctggcgc ttgaggattt gctatagaaa
    14401 cgttatcgta aatactctga ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt
    14461 taataattgt gtcttttgaa tagtgattta aaggcttacc accaatttta atctgtccct
    14521 tatccacatt gtagaaacct gaaattagtt tagctatcgt actctttccg cttcctgatt
    14581 ttcctactaa tgcataaatt cttttttgtt ctaaaactaa gttgaaattt tctaaaacac
    14641 actcttcttt gtatgaaaag ctgacatttt caaatgcaat gtcacaattt ttaatctgtg
    14701 tctcgttccc atattttagt tggtcttgct tcatttctaa ataaagggag tctagtgttt
    14761 cgactgcata tcttgcttca aacaagtgca tacttacata cattattctc atgaaagaaa
    14821 caaaaataat tccacttaaa aaaaacaaca tcaacaagtc aacagctaat atcttgggag
    14881 tggtaagaga agttataaaa aaggttatag gaactatcaa aatgcaaact atgccaaaaa
    14941 atagccattg aaaaagaaca aaaggtgttt tcccactcaa agaatactta tatgcaaact
    15001 tagcataatc gttaattgct ttattcaagg ctttaaatga tgttacttta gcaccaaata
    15061 tctttataac ttgaattccg cggatatatt ctaccgtttc agagcttaac acatctaacg
    15121 attcttgata tttttgaata aaatttgtat tctctgtcat tttctttaac aagaatacac
    15181 caattactaa catggcgatt agaaccaatc caattttaaa gttaatgaaa aagcctaagg
    15241 caactgataa aacaggtgtt actatggctt gagcactatc tggaatcaag tgagcaattg
    15301 ccatatgtgt tttagccgcg ttgtcatcaa ttgtctttcg aatatatccc gaagaattta
    15361 aatcaaaaaa acgaaaactt gcttctgtta aaccgctaat tccctttttt ctcaaattag
    15421 tctctaagcg aaacccaagg atatgagaaa ataagccaga aaaaatataa aataaagctc
    15481 taaagttaac caagcaacag tttgaaccgc ataactctta gctaaatatt catctcctac
    15541 aataattaga gactgaagaa atttgtaaat actgtaatat ccatagactg ttaatacgac
    15601 agaaaaatca gaaaataaag tggctaacca tccgtagtat ttcattttag ggacataact
    15661 aaataatttt ttgtatactt tcattttttc ctcccccgca ttcaatttct tctcttattc
    15721 gtttacaact gttaatgctt tccgtaatag cgtaaattag agatgtacaa acagataaaa
    15781 ggtaaactac gttctatttt ttgtattttt ttaagacttt tcttccaatc aatacaccaa
    15841 tcatagataa acttgctaca actaacactg aaatgattcc ccatatacct accgcatagt
    15901 ggatcattgc atccattgtt gcagcagatt gtcctttatc tatccaatct tgacgaaaac
    15961 gatctgtaaa gaaccatata ggaatccact gtccggcagc actgcctaat tgcattagag
    16021 cccaagacgc aacttgagga attgctcgat catatccaaa tacagataga ataatatcag
    16081 ctaagatcgc catactaatc gaactgataa accaaggtaa gtacgccccg cccattaacg
    16141 taaaaaataa caataaaaca attgtgtaaa tagtaaaaat tccttttcta gggtagttat
    16201 agctaatata aacaaatatg attccagata ataatccaaa aatcccgggg gatatgctgt
    16261 gaccaaacgc cccaccaata ctactaacca ttattgaaaa aaagtaaatt actagggaaa
    16321 taactgtagc taaaccaata aaaatataat cttttgtttt catatatatt ctcctcaatt
    16381 ctatttcgtt atgtcattaa aaatctgttc tatcaaagtt gaatcttcta ctctaagttt
    16441 tatatctttt tttattccac cattttttaa ataaataatt ctattgcatg ttttataaag
    16501 aaattccgga tcatgagtag ctataactac tgcacccttt tcttttaagt ctttaataag
    16561 gtcagcaatc ctttccatat taatataatc caacccactt gtaggttcat caaatagaaa
    16621 taaattagtt tttgacaatg aagctataga aaccaataat ctttgttttt ctccaccgga
    16681 taaatcaaac ggtaattgat ctttcatttt ataaagttcc atttttttta aaattttttc
    16741 ttgctgatct ttactaacta tttcattccc cagggctaac tcgctcctca ctgattctgt
    16801 gaaaaattgg taatccattt cttgcattac taaaaacggc gatgacaaat tttttttatt
    16861 ttcgattatc tttcctttgg tagggcgaat aatcttcatg agtattctta gtaaggttgt
    16921 tttccccaca ccgtttttcc ctaccaaacc aataacatct ccaactttga catcaaaatt
    16981 tatgtctttt agaatatttt cataagtaat ttcttcaact tttaatacat ctgcacttac
    17041 cttttcgata tagggaactt caatcgaact attaaaaatg tcaaatttcc ttaatgcgtt
    17101 attgttttca gtttttaaca actgaatagt tgtagttttt acag
//
LOCUS       DAZKBH010000053        17140 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000053 DAZKBH010000000
VERSION     DAZKBH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.053"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1475
                     /locus_tag="ACG8OO_001660"
     CDS             138..1475
                     /locus_tag="ACG8OO_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFP9045539.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ACG8OO_001661"
     CDS             1523..3652
                     /locus_tag="ACG8OO_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFP9045540.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ACG8OO_001662"
     CDS             3627..4316
                     /locus_tag="ACG8OO_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045541.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ACG8OO_001663"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ACG8OO_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="ACG8OO_001664"
     CDS             complement(5546..5740)
                     /locus_tag="ACG8OO_001664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045542.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="ACG8OO_001665"
     CDS             complement(5790..6230)
                     /locus_tag="ACG8OO_001665"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP9045543.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="ACG8OO_001666"
     CDS             complement(6234..7079)
                     /locus_tag="ACG8OO_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFP9045544.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="ACG8OO_001667"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="ACG8OO_001667"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFP9045545.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="ACG8OO_001668"
     CDS             complement(8703..9650)
                     /locus_tag="ACG8OO_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9045546.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="ACG8OO_001669"
     CDS             complement(9873..10223)
                     /locus_tag="ACG8OO_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9045547.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="ACG8OO_001670"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="ACG8OO_001670"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFP9045548.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="ACG8OO_001671"
     CDS             11646..11966
                     /locus_tag="ACG8OO_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9045549.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="ACG8OO_001672"
     CDS             11988..12452
                     /locus_tag="ACG8OO_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9045550.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG8OO_001673"
     CDS             12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG8OO_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFP9045551.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="ACG8OO_001674"
     CDS             complement(13301..14590)
                     /locus_tag="ACG8OO_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFP9045552.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="ACG8OO_001675"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="ACG8OO_001675"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9045553.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..15825
                     /locus_tag="ACG8OO_001676"
     CDS             15700..15825
                     /locus_tag="ACG8OO_001676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045554.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            15822..16250
                     /locus_tag="ACG8OO_001677"
     CDS             15822..16250
                     /locus_tag="ACG8OO_001677"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFP9045555.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQLNKYQIYIERN
                     YSNYKKIFVFLTPDGNEPTNPSWGIITYKNIVEESVKYFV"
     gene            16321..>17140
                     /locus_tag="ACG8OO_001678"
     CDS             16321..>17140
                     /locus_tag="ACG8OO_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFP9045556.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT         5455 a         3105 c         3466 g         5114 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttgaagaga gtgtaaaata ttttgtgtaa atagaaaaaa
    16261 ggaagtccct tctgtagaat agagttacca caacacattc acagaaaaga ggacttccat
    16321 atgaacgatt ttactacaga aattatggaa atactaatca ataaaggtga tttggatgat
    16381 ttgtttcgtc gtcacctaga attggctatt aacgctttat tacaagctga attaacggta
    16441 tttcttgact atgaaaagta tgatagagct ggatttaatt caggtaattc ccgtaatggg
    16501 aattattcgc gctcttttaa aacagagtac ggtgaattga atttagtaat acctagagat
    16561 aggaatggag acttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta
    16621 gaaaccacta ttattcagct agttcaaaaa ggaattacta tgtccgaaat ctctgatcta
    16681 atcgaaaaaa tgtatggtca ttattacaaa ccacaaacta tttcaaatat gagtaaaatt
    16741 gtgtctgaag atgtttcggc atttaaagaa agaactttag aagctaaata ctcagtcatt
    16801 tttatggatg ctactcatat tcccttaaaa agacaaaccg tatcaaaaga agctgtctat
    16861 atagtaattg gtatcaggtt ggatggaact aaagaggttc taggatttac tatagctcca
    16921 actgaatctg cttatgtttg gaaagagatt cttcaagatc taaaagaccg tggtttagaa
    16981 gaggttttgt tagtcgtaac agatggttta agtggtattg acgatagtat tcatagtgtt
    17041 tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag
    17101 gttcgtgtta gtgatcgaca agaaatctgt agcgatttca
//
LOCUS       DAZKBH010000054        17110 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000054 DAZKBH010000000
VERSION     DAZKBH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.054"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..226
                     /locus_tag="ACG8OO_001679"
     CDS             <1..226
                     /locus_tag="ACG8OO_001679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045557.1"
                     /translation="KNEKSWHLAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILKN
                     ENFFVSVLMGESLILILILCLIVHEVNKQL"
     gene            650..913
                     /locus_tag="ACG8OO_001680"
     CDS             650..913
                     /locus_tag="ACG8OO_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045558.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            1247..1618
                     /locus_tag="ACG8OO_001681"
     CDS             1247..1618
                     /locus_tag="ACG8OO_001681"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9045559.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            1921..2220
                     /locus_tag="ACG8OO_001682"
     CDS             1921..2220
                     /locus_tag="ACG8OO_001682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045560.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            2221..3675
                     /locus_tag="ACG8OO_001683"
     CDS             2221..3675
                     /locus_tag="ACG8OO_001683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045561.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(4511..5182)
                     /locus_tag="ACG8OO_001684"
     CDS             complement(4511..5182)
                     /locus_tag="ACG8OO_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9045562.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            5499..7130
                     /locus_tag="ACG8OO_001685"
     CDS             5499..7130
                     /locus_tag="ACG8OO_001685"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9045563.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            7133..8212
                     /gene="uxuA"
                     /locus_tag="ACG8OO_001686"
     CDS             7133..8212
                     /gene="uxuA"
                     /locus_tag="ACG8OO_001686"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9045564.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            8286..9713
                     /gene="uxaC"
                     /locus_tag="ACG8OO_001687"
     CDS             8286..9713
                     /gene="uxaC"
                     /locus_tag="ACG8OO_001687"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9045565.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            9797..10444
                     /locus_tag="ACG8OO_001688"
     CDS             9797..10444
                     /locus_tag="ACG8OO_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9045566.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            10573..11949
                     /locus_tag="ACG8OO_001689"
     CDS             10573..11949
                     /locus_tag="ACG8OO_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9045567.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            11960..12754
                     /locus_tag="ACG8OO_001690"
     CDS             11960..12754
                     /locus_tag="ACG8OO_001690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9045568.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            12767..14533
                     /locus_tag="ACG8OO_001691"
     CDS             12767..14533
                     /locus_tag="ACG8OO_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFP9045569.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            14561..16369
                     /gene="uidA"
                     /locus_tag="ACG8OO_001692"
     CDS             14561..16369
                     /gene="uidA"
                     /locus_tag="ACG8OO_001692"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP9045570.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            16541..16942
                     /locus_tag="ACG8OO_001693"
     CDS             16541..16942
                     /locus_tag="ACG8OO_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFP9045571.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            16936..>17110
                     /locus_tag="ACG8OO_001694"
     CDS             16936..>17110
                     /locus_tag="ACG8OO_001694"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045572.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITY"
BASE COUNT         5976 a         2329 c         3414 g         5391 t
ORIGIN      
        1 gaaaaatgaa aaaagttggc atttagcaca aaaattgttg ggggattttt caaaaaaaaa
       61 agttatccca gtatttttga tgggtattgt ttttttcagt atagagattt atagtttagc
      121 tattttaaaa aatgaaaatt ttttcgttag tgtattaatg ggtgaaagtt taatcctaat
      181 actgattttg tgtttaattg ttcatgaagt gaataaacaa ttgtgaatta aaaaaataaa
      241 aacctagtat aatcacttta ataaattgtt ggtagaaggt gatcaaaatg ggagtaaacg
      301 aaatgatgaa agtcagattg agtttttaaa tgataccttc ctcagaactt caaaacgaat
      361 tcaaagattc gttgacaaat cttgagcaag atcatttaaa aaaatatttt ctcttcattt
      421 gacgaaaaat gtttagaaat cttatataag tgaagataaa gtaattggtg taattaattg
      481 tagagaatag cctttgagca attgaagcga tacaattatt tagaaaatga acttcctcca
      541 gcatactaca tgattcctta cgggacgatg ttaaagaaga aagcaagaaa ttttagaagt
      601 attagtagat aaactaatgg gcttttctca atttaaggga gtagcccaaa tgtttatcaa
      661 atatggtgca gatacagtag caaccatgtt tacagaagaa tatcaagtag actattatga
      721 agtaatagat aaatgtgtac attttgttta tgaaaaagtg tggaatttta tgaattttgc
      781 gataagtgaa gaaatcttac gagtacaaga aaacagaaca ggaatattta ccagagacag
      841 aaagcttaat ttgattgcgc attatatacg gaatcgctgg tcatcaatac caaattttaa
      901 ttataaaaaa taattatgaa caatctttta tacaatatac acaataaatc aagtagtatt
      961 tatgaaaaaa gacttcaaaa acagaatgat tttctatcag tcttagaaat tatagagcta
     1021 aaaattttgg tcacgcgtag aaacgcgttt tttttgtttt tttgatagta aagaacgtac
     1081 aaaacaatga gaatttaaaa gattgtacta ccagtttttt taccttttca tttgagttta
     1141 gagtaagtaa aatattacta taaaaacaca tattcttgaa gaaatctttt ttttgctgta
     1201 ttcatttaga ttatgataga gaaaatacgt gagaggggga cctgggatgt ggctatctga
     1261 tgaaaaaatt cagtggcgta gctatcagaa gcgtatattg actaactttg ctacttattt
     1321 aaataacaat tcagtccatc tagtggctcc accaggggct ggcaagacct ttttaggaat
     1381 tgagatgatc cgacgtttgg attgtaaaac cttaattttg gttccttccc taatattgaa
     1441 gaaacagtgg ttaacaacaa tcgaggaata tttcttagta aatccagcag atagttgcta
     1501 tctttctggg gcgattgaac atcctagtca tattacagtg gaaacgtatc agaccattta
     1561 caaccgactg aaggaggagc caacttatgg tgatcagctg gagctgttag ttttttgatg
     1621 aagctcatca tttaaaaaaa agctggggtg aattgttgat tgacttgaag aaaaaacaac
     1681 cgaatctgtt gacgatttcc ttgacagcca cgcctccgat tgatagctct cagacggagt
     1741 ggaagcaata tattgagtta aaatggcctg attgacgaag aaattagcat tcccgagttg
     1801 gtaaaggagg aggttctatg tcctcatcag gattttctat atttgattcc ggctaatcag
     1861 gaaactactc agcattattt taccttttta gagaaacaaa atgagctggt ggaagcgtta
     1921 atggaaagcc aagagacggt ggattatttg ctttctttag aatttgttca gcatccgctt
     1981 gagcattcga aggaaattta tgaaaatttt gagctgttca ctagtgcatt aatttacttg
     2041 aatcatcata actatgaatt gatacccgaa cattggcagc tattaggcag ttctaagcag
     2101 caaggtaaaa aagcttcagt tccatcttta ggtaaaaaag agctgatcac tatttatcag
     2161 cagatgtttg aagatcaacc ggaattgcct attttttatc aattgaaaaa agctggatga
     2221 ttgtttgatg agtcgctgca tttgtttcca gagtatccag acactgttgg tagtcaggcg
     2281 attcctgaga aaaagcaagc agtttgtcaa attttgttaa aagaagaagt tcagctgaag
     2341 caagaactga cagcggtcat tttgatggat cgtatttgca aggaggtatt agcaggcagt
     2401 gatggtacct tagcttatgg cgtggttcct ttgtttgatg aagttgttcg tctattaggt
     2461 aacactcagc aaatcggtgc catctgtgga gagttcttac tgttaccgaa aatgctagta
     2521 actgttgagc tggcagaact agcgacgggt atgaatgaac atccttatat gtcgggttat
     2581 ttttatttga cggtggatga caaaaaccgc caagcattga tgcaagcagt tactcgccta
     2641 ttaaccgaaa agaagttgaa tattttggta ggaaccgtta gtttgctagg agaaggctgg
     2701 gattgtcctg caattaatct gttgatcttg gctaatcaag cttccacttt tgtgcaaaca
     2761 caacaactac gagggcgggg cttacgccta ggtgcacaaa ataaagtaac gaacatttgg
     2821 catttagcga ttggattacc gggagttcct ctgtctgaac agcctgattt gaaaagaatg
     2881 ctacggcgtt tgggcaatat ttcaggtctg acctttgaat cttcaccaaa aattgagagt
     2941 ggtggcgatc gctttgcttt accactggaa tatacgacca ccacgatgga aacttatact
     3001 cagtcgatgt tctatttttc ttgtgaacga agtcggttga aagaacaatg gagtactgct
     3061 ttagctgatg gctggcacct atctatgcca atcgtcgtta ggaaagtcga tcaggtaaag
     3121 caaaaacaag aaacttcatt gccgatagta gtaaaaaata tagagcctct aagcttctgg
     3181 tcagctatat ttaaaggtaa cttgctggaa tatttccaaa ttcgtaggcg acaaaagcta
     3241 tgggaaaagg aatgtgcccg caaaaaaagc ttggccatat gcgttcaaac gacgttgcag
     3301 cgccttggtg aagttagtga tagaaaagta atcgtagcgg ctgatcagca ggaatttaga
     3361 tgctatcttg agaatgctgg ctatcgagaa ggacagcttt ttactgaagc tatgagggag
     3421 cttttagcac cgctggagaa tccgcggtat ttaatcgaag ggaaaaagat agttctgacg
     3481 gtaccagaac gctttggccg aaataagcaa atcgctaacc tttttctaaa cgaattaaat
     3541 caatcgtcgc tgcccaaatg tcagttggtg tatactagaa atcctgaagg acgccaaaag
     3601 ctagtgactt atcgaatgag gcaagtcttc cagacccgcg atgaagtaat tattcaaaaa
     3661 aacatttgga cgtaagttga tgaaaataaa ttttgctaga gtagaacaag tccgagaatt
     3721 aagttgctac ttaactgata atgccaagga caggagaatc ttttcttgac gactgaaaga
     3781 aactatttga cttatataac ggtgtgtgat gaatatttgc aaggcattct cttaaaaagt
     3841 actcgtaata gtttttttga aaacaaaaca gtactttatt aataggactg acagagcgat
     3901 ataagaacat ctgcagacag aggtgaatgc ttttctcatg atcgatgaaa caatggtact
     3961 tcggatattc ttgcttttag tttgaaccgt tgtgattttc tttgattcct aaaagtagtt
     4021 ttatagaatg aaaatcaagc atacggaatg ctcctttgat tgtgttgtgg tgattcaatc
     4081 taatggagct tctatatgtt tttttatttg caaaaagaac cgatagaata tttttatcag
     4141 tcttagaaat tatagagcca agaaatttca agcacacata aaaacgcgtg ttttatgcat
     4201 tttatgagca aaaataacta aagaccatta cgtaaaacat aggtaaatca gcattcaatc
     4261 tcaaattatg tgatcaaatg ttgatattat ttgattataa gttttctttt gctattatca
     4321 gtctgcaaat aaacagatta tagagtatga atgtagatag ttcaatcttt gtttacgacc
     4381 tcacatgttt ttttactcaa gagaaagtaa tataaagtga ctatatttga cagaacttta
     4441 cataaaaaaa gtagttagtt ttctgtagta atcagaaatg taaataaatt aaaaaaaaga
     4501 aatttagcag ttatatattt ttaaaatatt ttgggtgagt agtcaataca acattttgtt
     4561 catcaagcat taaatgaaga tgcaaggatg ttaagaagtt agcttcttca gggtcttgca
     4621 tggtcaaggc tttaaatagt tgatgatgtt gttccataat tttttcccat tttaatcctt
     4681 ccgtttttac tcgtaaccaa cgaaatcgtt ccaagtgcgt attattatct tccaaccatt
     4741 gccatacttc ttcccgttct gcaatttcaa aacaagcttt atgaaataaa ttatcattat
     4801 gaaaaaaact acgagcattc ttttgactaa cagctttttc ttgttctgca attatagttt
     4861 ctaaaacttg tttcttttga ttattcatct tggcacaaca ctctagcata atttgtcgtt
     4921 ctaaatgttc tctgacaaaa cgagcatttt ttgctaaatc taagtcgatt aatgagatat
     4981 aatttccact ttgaggaatt gtatcaacaa gtccttgatg acggagttga atcaatgctt
     5041 cacgtattgg agtacgaccg atagaaagcg tttcttccaa ttttttttct gatacttttt
     5101 ttccaggttc taagtcagcg tagataattt gctgacgaat agattgataa gcttgatttt
     5161 ggagactagt tttaattgtc atatttaatc cctctcaaaa tttcattttt gaatttgata
     5221 ttttagttct atgctcgtta tataatatca aatttcaaag taacatacta gctattaaaa
     5281 aaacaaaaaa tactcaaata agatcaaaga gaaattaagc tttaatttaa gttagttcta
     5341 aaaaatatat attggtgtgc ttttactaaa aaaagacaca ataattctga catggtagaa
     5401 aaaagaatat ttttagtttt ttcttattga cagcgattac attcgatttt atactacaat
     5461 tgtaacggaa actaacatat cagtatggag gggtaaatat ggttttgatt aaagactggc
     5521 aagattctat cgaaggattg aacaaaatgc aagttcaact accgtatttt aatgtcgaag
     5581 cggtcaaaca agaaggtcag aaacatccag tgtggcttca ttttggtgga ggaaatcttt
     5641 atcgaggatt tcatgcagaa attgctcaat gcttagcaga tcaaaatgaa ttagaatccg
     5701 gagtagttgt gtgtgagaca tttgatgaag aagtagtaga aaaagcttat catgactaca
     5761 acaatgatat tttggagatt gttatgggtg aagatggtga actcaaaaaa agacttttag
     5821 cagtaacagc tgatagttta tattgcaatc caaagaatat aacggactat caaagagtgg
     5881 ttaaatattt tgaagatcct acattacagt ttacaacgtt tactattact gaaaaaggat
     5941 acagtttaaa agattctaaa ggacaatttt tttcggtagt ggaagcagat ataaaatctg
     6001 gacctaccaa tcctcaacat acgatgagta ttgtgacatc cttgttgtat agcaggttta
     6061 atgctggaga gttaccgatt gcgatggttt ctactgataa tttctcacag aatggtcaac
     6121 gttttcaaga tagcattttt atgattgcag aagcttggca aaaaaaagga tacgtttctg
     6181 aaaaatttat tgcttattta aaggataaag aaaaagtgac ttttccttgg agtatgattg
     6241 atagaattac gccaaatcct tcggaaagtg tcaaaatgga tttattgaag gcgggtttcg
     6301 aagacttaga tataattcat actgcgaaaa ataccaatat cgctgctttt accaatactg
     6361 aaaaagtcca ttatctagtc attgaagata gttttccaaa tggtcgacct aaactggaag
     6421 cggctggtgt gattatgaca gatagagaaa cagttgataa agcagatatc atgaaggtta
     6481 cgacatgttt aaatccgctc catacggcat tagcaataac aggttgtttg ttggggtata
     6541 aatcaattca ggcagaaatg caagatgatg atttggttgg cttaattaaa gggataggtt
     6601 atgtagaagg cttaccagta gttgaaagtc caggcgttat tgatccaaaa caatttattg
     6661 atgaagtaat caataaacgt ttgcctaatc cctatattcc agatacaccg caacgaattg
     6721 caacagatac ttctcaaaaa gtagcgattc gctatggtga aacaattaaa aagtatgtcg
     6781 aaaaagacgg aaatgcagaa aatctaacat ttattcctat cgctattgct gcttggttac
     6841 gttatttatt ggcagtcgat gatcaaggaa aaaattttgt gccaagtcct gatcctttat
     6901 tggtagagtt gcaggaaaaa ttctcagtat tgaaattagg tgaacaagat cctgcaaaga
     6961 ttcattgtgc ggtacaggaa atcttagaaa ataaggaaat atttggttta gatttatatc
     7021 aagttgagat aggaaagaaa attgaaaaaa tcttttgcga aatgctagta ggtcctcaag
     7081 cagttcgtaa aacaatcaat aaatatataa cagaagttgg aggaaagtaa ctatggaaat
     7141 gacttttaga tggtatgggg caaatgaaga aaaaatctca ctacaagaaa ttcgtcagat
     7201 cccagcaatg aaaggaattg ttggaacatt aatggatata ccagccggag aggtctggcc
     7261 gagagaacga attaaagctt taaaagatga agtcgaagcc aatggcctta ccttgaaagt
     7321 tattgagagt gttaatgttc atgatgatat caaaattggt ttgccaacac gagatcaata
     7381 catcgagaat tataaacaga cgattcgcaa tttggcagaa tttgggattg aggttatctg
     7441 ttataatttt atgccagtct ttgattgggt aaaatcagat ttggattatc atttagatga
     7501 tgggtcttct acgttagcct atatttcaaa aaatattcct actgatccag aggaaattat
     7561 tagaaaagta gaaaattcta ctaataattt tgaattgccg ggttgggaac ctcatcggtt
     7621 atcacaaatc aagcaattat tagaagccta taaagaagtt gatgaagcga aattgcgaga
     7681 gaactttgca tattttctca aagcaattat cccgacatgt gaagaagttg ggattaaaat
     7741 ggcagttcat ccagatgatc caccatattc tattttcgga ttacctcgta ttgttaaaaa
     7801 tcgtgacgat ttagaatggt tgtgtaatgt agtggattct ccttctaatg gtattacgtt
     7861 atgtactgga agtattgcag aacgaccaga taatgatgtc tatgaaattc tatctgaatt
     7921 ttgtcaacgg gatcggattc cttttgcaca tgttcgtaat atcaaatatt taagtgataa
     7981 tggactcgat ttttatgaag cgccgcataa atcagaatat ggttctctag atatgtataa
     8041 gatattaaaa acaatgtacg ataatggatt taatggctat attcgtccag atcatggtcg
     8101 gatgatttgg ggagaaaccg gtagacctgg ttatgggtta tatgaccgag ctctaggtgc
     8161 agtttattta aatggtattt gggaagcgct tgaaaagggg gcgagtaaat aattatataa
     8221 tggagtctat ttagaagaaa aattttctct tctaaaaact aagggaagga aaggagtgct
     8281 taaatatgtt tttagataag aactttttat tagagaatga aatagctaaa agactttttc
     8341 ataaatttgc agaacaccaa ccaatcatcg attatcattg tcatttagac ccagcatgtt
     8401 tgctagaaaa taagcaatac aagaatttga ctcaggtatg gttaaatgat aaaggagctg
     8461 gagatcatta taaatggcga ttgatgcgag ctaatgggat agaagaagag ttgatcagtg
     8521 gtgcaggtaa tgattatgaa aaatttttag cttttgtaaa aacagtggag aaggcgccag
     8581 gtaatcctat ttttgaatgg tctcatttgg aattacaacg ttactttgat attgatttga
     8641 caatttctga gaagaatgca ccagaaattt ggcgacgtgc caacgaaaaa atccaaacgg
     8701 attcctatcg cccaaaagaa ttgctgaaga tgatggatgt gaaagccatt tgtacaacgg
     8761 atgatcctac agatgacttg cgatatcata ctgagctttc taaacaggat aattctttca
     8821 aggtattacc tactttccgt ccagataagg tctggagtat caaatcatca ggattttcga
     8881 actatatttt aaaacttagt caagcagcgg gtataccaat caaaaacttt aatgggttga
     8941 agcaggcatt gagccaacga gtggattatt ttcatcaaat tggctgtcga ttagcagatc
     9001 acggtttgaa tacttatttt tatgaaatag cagatgaaaa aatactcgac aatctgttaa
     9061 taaaagcttt agcaggaaaa acggatttcc atttatcaga aattgcacaa tttgttacag
     9121 gtattcaatt acatttgatg tctgagtata caagatatgg ttggacgatg caaatgcatt
     9181 taaatgcttt aagaaataat tcaagtatat taaaggaaca aattggagta gatgtcggtg
     9241 gtgattcgat gggggatcaa gctagtttga cacaaaatgt tgtagcattg ttcgacactg
     9301 ctgaaaagag tgggtgctta ccaaaaacaa ttttatattc attaaatcca accgattggc
     9361 ttccactagc aacagcaatg caaagcttcc aagggggtat gattcaaaaa atccaattgg
     9421 gatgtgcttg gtggttcaat gatacagctg aaggaatgcg tcaacagttg actacgatgg
     9481 cgcaacaaag tttgttgtcg aattttacag gtatgttgac tgattctcga agttttctaa
     9541 gctatcctcg ccatgagtat ttccgtcgag tattatgtca attactaagc gagtgggtta
     9601 aagcaggtcg aattccagat gatgaaatgt atcttagtca gatagtggag aatatttgtt
     9661 atcgtaatgc gtataattat tttaattttt ttgaaatcga aagtagaaaa taatacaaaa
     9721 aataaaagct taaatttatt tattgtaagt tagtacaact agctagagta tgtatccata
     9781 aaagaaggag atagatatga aaaaagtaga aattttaagt aagttagaag cggctggagt
     9841 cattgcggtt gtacgtggaa aaaataaaga tgaggccatt aaagcaataa atgcaattac
     9901 tgcgggtgga attaaaggga ttgaactaac ttttacagtt ccgaatgctg aagaaataat
     9961 caaagaaatt gctaaaaaat atgctgatca aaaagatgta ataattggag ccggtacagt
    10021 tttagatgcg tttactgctc gtattgcaat tatggctgga gcagaatata tcgtaagtcc
    10081 atcttttgat cgacaaacag cagagatttg taatttatat caaattcctt atttaccagg
    10141 ttgtatgaca attactgaga taaaggaggc acttaccgct ggagtagaaa tcattaaact
    10201 atttccaggt tctgcttttg gtccatctgt tgtaaaaacg tatcaagctc cattaccata
    10261 tgtaaatata atgcctactg gcggagtaag tctagataac ttagaagagt ggtttgaagc
    10321 aggcgttgtt gcagttggtg taggtgggaa tttgttggct cctgttgaaa aaggtgattt
    10381 tgaagaagta actcgaatag ctagtaaata tatagataag ttttatgaga taaagaatta
    10441 ttaggagaaa aaataaataa tactataaaa tgtaaaaaat aaatactaac tctttatact
    10501 tcagagtata gtacgtaagt tggcagttca acagaatgaa aacgcttaac aataggaagg
    10561 agcgcaaatt atatggaaag aaaatttggt ctaagagaca aaataggata tatgtttgga
    10621 gattttggga atgatttcac tttcattttt gctagttctt ttttaatgat tttttataca
    10681 aaagtattgg ggataagcgg agctaccgta ggaacattat ttttagtagc aagatttatt
    10741 gatgcagtta cagacataac aatggggcgt attgtggatg gatcaaaacc atctaaaact
    10801 ggcaaattta aaccgtggat tttacggatg agtggtccag tcgctttagc aagtttttta
    10861 atgtatcaaa catcaatggc atcagcttct atgagtatta aagttatata tatgtatgta
    10921 acatatatac tatggggaag tattttttat acatctatta atattcctta cggttcaatg
    10981 gcatcagcta tcacttctgt tgcagaagaa aggacagcgt tatcaacatt tcgtagtata
    11041 ggagctacct tagcaggtat gttcattggc gtgttaacac caatttttat ttatacaaga
    11101 gatagttctg gagctcaaat tgtacgtggt ggatcaactt ttacaattgt ggcaggagta
    11161 ttttcagtgt gtgcagttat ttgttacgta atatgttata aattaactgt agaacgggtt
    11221 aaaagtgatg aatcgcagtt aaaagtttca ttgttgaaat ctttaaaagg attagctaaa
    11281 aatagagcgt tgctttctat aatagctgca gctatttttt tgttgcttgc tcagctttta
    11341 acaatgagta tgaacaatta tgtatttcca gattattata ataatgcaca gggaatagtt
    11401 ttaatgaact ttatcaatcc tattttgatg ttggtacttg tttcacctgt aagtttaatt
    11461 ttcagtaaaa aatacggtaa aaaagaattg gcgtcagtag ggatgttctt ctctgctatt
    11521 gtatatttac ttttattttt gataaagcct agtaatatgt atgtattctt agttcttact
    11581 tctgttggat atatggggtt aggtattttt aatacagtca tttgggcgaa tattactgat
    11641 gtaatcgatg atcaagaagt gaaatctggc caaagagaag atggaactat ttatgcagta
    11701 tattcttttg cccgaaaaat tggacaagct ttagctggcg gtgtcggagg ttggactctt
    11761 tctataatag ggtatgattc gttagcaaaa gtacaaagtg gagaagtttt acaaaagctg
    11821 tataactctt caacgttgat tccagcagtt tgtttctttg ttgtaggatt gattttattt
    11881 ttcgtatatc cattatctaa agaaaaagta ttagaaaatt ataatttgct aaatcaaaaa
    11941 cataactagg agaaaaaaca tgaacagttg gatattttgt attgattcag atggttgtgt
    12001 catggataca atgacttata aacacaatct ctttttcggg ccaattgctg cagagatttt
    12061 tgatgttcat catagggaaa aattcctaca ggattggaat aatattaatt tatattcaga
    12121 gactcgtgga ataaacagat ttttaggttt agttaaaaca ttagaaaaaa atgatgtacc
    12181 atcgattaat cgattaaagc actgggtatc tgtaacaaaa gaattgtcta atccatcttt
    12241 gaagagggaa atagaatact ctcagtcaga ggatttatta aaagctttaa aatggtcgga
    12301 agaagtcaat cgacagatta gtcaagctaa aggattagat aaaccctttt taagtgctag
    12361 agatactata aaagctctga aaaaatatgg gaagataatt attgtaagct cagctaataa
    12421 ggaagctatt caagaagaat gggaaagaca tgaattatta tccttagttg atgaactgtg
    12481 ttgtcaagat aaaggaaaaa aagaagacat tattcgaagt gtaattgaaa atggttgtga
    12541 tttggataag atattaatga ttggtgattc tcctggtgat ctagaagctg caaataagaa
    12601 taaagttttc ttttatccta ttttggttaa taaagaaaaa gaatcatggg aaaacttaag
    12661 aactgatatt ttatttaaat gtttatcggg agagtatata gatattgaac aaaaatatat
    12721 agatactttt tggaaaaatc ttactaataa gtagaggaga aagaaaatga ctataaaagt
    12781 agacttacga aaaaaaccat atcatttaga tgaagatgct atttcttgga ttgaagagac
    12841 gattgatgct atgactttag aagaaaaaat aggtcaatta tttgttaata tgggatctag
    12901 tcgtgataaa aattacttaa gtagtatggt caatcagtac catattggag ctgtacgata
    12961 taatccagga actgctgatg aggtatatga tcaaaactat atcctacaaa cagaaagcaa
    13021 aattccttta ttaattgctg ctaatacaga agcaggtggt aacggagctt gtacagatgg
    13081 taccgaagtt ggtgtagaag taaaaattgg tgcaacaaat gatgcgaaat atgcttatga
    13141 attagggaga gtttctggaa ttgaagcatc ggctattgga tgtaactgga gttttgctcc
    13201 tattgtagat attaatagaa attggagaaa tcctatcatt tctagtcgat cttttggaca
    13261 aagccctgat aaagttttag aacttagttt agcttatatg aaaggtattc aagagagtgg
    13321 aattttgcct gcagctaaac atttccctgg tgacggtatt gacgaacgag atcaacatct
    13381 atcttttagt attaatagtt taagtacaga agaatgggat aattcttttg gaaaagtata
    13441 ctcaggctta atagaagctg gattaccttc aattatggca ggtcatatcc atatgccagc
    13501 ttattcaaaa cattttaatc ttgatctgaa agatgaagat ttacttcctg caactttatc
    13561 aaaagaatta atcactgact tacttagagg aaaactcggg tttaatggat tagtagttac
    13621 tgatgctagt catatgttag gaatgactgg ggcaatgaaa cggtcagaat tattgcccac
    13681 ttctattgcg gctggatgtg atctattcct attttttaat gatccagatg aagactttaa
    13741 ttatatgatg gaaggttatt acaaaggaat tatttcagaa gagcgtttac gtgatgcatt
    13801 aagaagaatt ttaggcttaa aggctaaact aggtttgcat aaaaaagaaa agaataaaat
    13861 tttgttaccg aaacaagaag ctatggaaaa aattggttta attgaaaata aatcaatttt
    13921 taaagaggta gctgatcaag caattacact agttaaagat aaacaagata tttggcctat
    13981 tgtaccagaa aaatataaaa ggattttact ggtagatgta catggggtaa aaggtgggtt
    14041 tggtgaagta gttgctggaa atagacctca ggccattgat ttattgaaac agattttgtc
    14101 agcaaaagga tttgaagttg aaaaatacat ttcacctgaa gaagaattga aaggattatc
    14161 gcaagaagaa aaaattaaaa atatagctgg aatatatgct gctaagagac caatcaaaga
    14221 tttaactgaa aaatatgact tgattattaa tatagctaat gttaacccag gtacagttca
    14281 aagaattgtt tggccagcaa gtaaaggtac tccggatatt ccattttata ttcatgaaat
    14341 tccaacaatt tttgtatctg tacagtgtcc gtttcattta gctgatgtgc ctcaagtaaa
    14401 aacttatatc aatacttatg atggaaaaca agaaacttta gaaatattag tggacaaact
    14461 aatgggcctt tctgaattta agggaataag tccaatagat gcttattgtg ggttaaagga
    14521 tacatttatt taatatttaa attatatagg agtgatttac atgttatatc ccattatgac
    14581 agagacgagg caagtaattg atttaagcgg tctttggaga tttaagttag atacaaatga
    14641 tgacttacaa gaagaacttg ctttagaacc aatggttgaa aaagagagtt atccaatttc
    14701 agttccttct tcttataatg atttatttga aacagagaac attagaaatc atgttggatg
    14761 ggtgtggtat gaaaaagaag tgattcttcc taaaaaatta ttagaagaaa gattagttct
    14821 tcgttttgga tctgcaacac atgaagcaaa agtatttttg aatggaaaat tgattaatca
    14881 acacaaagga gggtttacgc catttgaatc agaaattaat cagtttataa aagatggaag
    14941 taacagaatt acagtggctg taaacaatgt tattgacgat agcactttac ctgtagggat
    15001 tttgaaagaa acgatacagg aagatggaga cataaaaaaa gaaaatttgg ttaattttga
    15061 ctttttcaat tatgcaggca ttaatagacc cgttaaattg tatacaactc cgaaagacta
    15121 tataaaggat gtcactatta atacaaaaat aattgattca gatgcaaatg tagaatttaa
    15181 tatcgataca gaatggaccg gagaaattct aatagcaatc tatgacgaag aagctcaact
    15241 tatagcagaa gctaagggga aagaaggagt gattcaactt aagaacccaa cactttggga
    15301 gcctctaaat gcttacttat atagtatgaa agttagttta attaacgaaa ataaagtgat
    15361 tgatacgtac acagaagaat ttggcattcg tagtattcag gtttcgaatg ggaagttttt
    15421 gattaataat aaaccttttt actttaaggg atttgggaag catgaggaca cgtatattaa
    15481 tggtcgtggt ttgaatgaag cagtaaatat caaagatttt aatttaatga aatggatcgg
    15541 tgctaattca tttaggactt cacattatcc ttattcagaa gaaattatgc gcttagcaga
    15601 tcgagaaggt attgtagtta ttgatgaagt acctgcagta ggattacatt tgaatttcat
    15661 ggctacttta ttagatgaca acgtagaaaa acatgatact tggaaagaaa tacggacaga
    15721 aaatgctcac aaaatggcga ttaaggaatt gatagaacgc gacaagaacc atccgagtgt
    15781 cgtaatgtgg tctattgcaa atgaaccaga ttcggattct gaaggcgcta aagaatattt
    15841 tgaaccttta gttgaattga ctaaagaact tgaccctcag aagagacctg taacaatcgt
    15901 tacttactta aagtcaacac cagatatttg taaagtagga gatattgtcg atgttttatg
    15961 tttaaatcgc tattatggtt ggtatgtttc aggtggagac ctaaaacatg caaaatatct
    16021 gttgaaaaaa gagctagatg gatggatgga aagatgtccc gatacgccaa taatgtttac
    16081 agaatatgga gcagatacag tagctggact tcatgataca gtaccaacaa tgtttacaga
    16141 agagtatcaa gtgggttatt acgaagcaaa tcacgaagta atagataaat gtgacaattt
    16201 tgttggtgaa caagtgtgga attttgcaga ttttgaaaca agtcaaggga ttttaagagt
    16261 gcaaggaaat aagaaaggaa tctttactag agatagaaag cctaaaatga ttgctcacta
    16321 tctaaagaaa cgatggtcat caataccaaa tttcagttat aaaaaataat ttacctttta
    16381 taaggaatat aaaaaaggag tggctgctta attgccaact cctttttttt ataaaaagat
    16441 tttctgttta aagaaggata tttttttcta gttttttcga aaaaaaggcg ctttctgatt
    16501 gttgtattag tggaaagggg aaaaaaggag catatttatc atgcaacaaa attacaatga
    16561 gaaatctata caacatcaat ttgatgccta ctgtaaaaaa gtattacgaa acgaaactaa
    16621 gtctattcga aaacgaaaca ttaacataac agataatgag gaaccattga cgaattcaaa
    16681 tgaaggtaag tactcccaaa ctcattttga tgaacaagat gtctatttct tatttggaat
    16741 ggaaatatta atttcagatc aaaagttatc ggcagccatt gatcatttat cggaaataag
    16801 aagaaaggtt atcctacttt attattttgc aggttttaat gatactgaaa tcggtaaaat
    16861 cctcaatatg agtacaagtg gtatctggta tcaacgaaag aaggcagttg aacaattaaa
    16921 aatggggtat gacttatggt agacaacccc aaaaaacggc agtatccatt agttcctcta
    16981 tccattatcc aatctgctct agaagaagat ccaatttcaa tcaagatgat actcaaacat
    17041 tatcatagtt atatagctaa gttatgtttg acaaatggat ttaatgaggc ggggcaattt
    17101 attacctatg
//
LOCUS       DAZKBH010000055        16790 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000055 DAZKBH010000000
VERSION     DAZKBH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.055"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..309
                     /locus_tag="ACG8OO_001695"
     CDS             <1..309
                     /locus_tag="ACG8OO_001695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045573.1"
                     /translation="TQISPAEFQVILFNSLYIKRGFGLGIQLIGSGFFGDDFDFETNQ
                     HFETSINEQWFLSLSTVDSDNSIKRQKLSEYIKELGSVEYKKIANFESLYKLFNETKL
                     "
     gene            353..1525
                     /locus_tag="ACG8OO_001696"
     CDS             353..1525
                     /locus_tag="ACG8OO_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9045574.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1512..2216
                     /locus_tag="ACG8OO_001697"
     CDS             1512..2216
                     /locus_tag="ACG8OO_001697"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP9045575.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2221..3381
                     /locus_tag="ACG8OO_001698"
     CDS             2221..3381
                     /locus_tag="ACG8OO_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9045576.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3457..3735
                     /locus_tag="ACG8OO_001699"
     CDS             3457..3735
                     /locus_tag="ACG8OO_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFP9045577.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3716..4087
                     /locus_tag="ACG8OO_001700"
     CDS             3716..4087
                     /locus_tag="ACG8OO_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFP9045578.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            4103..4507
                     /locus_tag="ACG8OO_001701"
     CDS             4103..4507
                     /locus_tag="ACG8OO_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045579.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            4504..4914
                     /locus_tag="ACG8OO_001702"
     CDS             4504..4914
                     /locus_tag="ACG8OO_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045580.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            4975..5562
                     /locus_tag="ACG8OO_001703"
     CDS             4975..5562
                     /locus_tag="ACG8OO_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFP9045581.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            5635..6102
                     /locus_tag="ACG8OO_001704"
     CDS             5635..6102
                     /locus_tag="ACG8OO_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFP9045582.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6148..6456
                     /locus_tag="ACG8OO_001705"
     CDS             6148..6456
                     /locus_tag="ACG8OO_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045583.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6651..13385
                     /locus_tag="ACG8OO_001706"
     CDS             6651..13385
                     /locus_tag="ACG8OO_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9045584.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            13416..14261
                     /locus_tag="ACG8OO_001707"
     CDS             13416..14261
                     /locus_tag="ACG8OO_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFP9045585.1"
                     /translation="MNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSFREIGESIEL
                     SEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSLKSLSKTEID
                     SQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYTKNVWEKKGV
                     FKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFINMKDTQILE
                     VSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGHANVEIELYE
                     ERDLF"
     gene            14258..15307
                     /locus_tag="ACG8OO_001708"
     CDS             14258..15307
                     /locus_tag="ACG8OO_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045586.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            15311..16135
                     /locus_tag="ACG8OO_001709"
     CDS             15311..16135
                     /locus_tag="ACG8OO_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045587.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            16153..>16790
                     /locus_tag="ACG8OO_001710"
     CDS             16153..>16790
                     /locus_tag="ACG8OO_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFP9045588.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         6003 a         2577 c         3571 g         4639 t
ORIGIN      
        1 acacaaattt ctccagctga attccaagtg attttattca attctctata tataaaacga
       61 ggttttggct taggaattca acttattgga agtggttttt ttggagatga ttttgatttt
      121 gaaactaatc aacattttga aacaagtatc aatgaacagt ggtttctatc actatcaact
      181 gttgacagtg ataactctat aaagcgacaa aaattatctg aatatattaa agaattagga
      241 tccgtagaat ataaaaaaat agctaatttt gaaagtttat ataaactctt taatgaaact
      301 aagctctaga aatagggctt ttttattttt ctttgaaagg tggtgaaaac atgtgagctt
      361 atttgatatc tttaagcagt caataagaaa tgaagagcct tctgagtgga taccggattt
      421 tgtatatgga gaggaagtat ctgctcgggc atacctcaaa attatggcaa aaaatactgt
      481 tttagatttt gttgcaagaa ctatgtcaac gttagaagtt aagtttaaaa ataaagatgg
      541 gacatcagac tgggattata ttctaaatgt tcgacctaat tctgatatgt cagcagctac
      601 attttggcaa aaattttttt atcggctctt agatgacaac gaagtgttag taatatttac
      661 agatgataat caattgctga ttgctgatga ttttagtcga acggagtatg ccgtgtatga
      721 tgatgttttt acaaatgtga tggtaaaaaa ttttgtcttt gaacgttcat ggaacatgtc
      781 tgacgtcatt tatatggaat ataataacga agaattagaa aggtttacac gaggtttatt
      841 tgaagattac gccgcgttgt tcggacggat tctagaagtt gcaatgcgta acaaccaaat
      901 tcgtgggtct gtatccattg attcaacggg aacggcgaat gatgaaaaag gaaaagatgg
      961 taagactaga tctcaaagat tacaagaata tattgatagg atttggaact cttttaaaac
     1021 taactcagtt gcaattgttc ccaaagtgaa aggctttgag tatgaggaat acaccaataa
     1081 acaaggcgta tcgaatcaat cgctagatga actgaataag atgaagtcgt ctttaattga
     1141 tgatgttgcc aacgccatag gagtacctac ggcgcttatt tatggtgaaa aagctgaact
     1201 ggattcaaat attaaagctt ttcgtaaatt gtgtattgct ccactagtta aaaaacttca
     1261 agacgaattg gtggcaaaaa taattagtcg tcaagagtac aaaaacgggg aacgtataga
     1321 agttaaaaaa gttctccaaa aagatctatt agagtatgct actcaaattg ataaaataac
     1381 atctggtggt gcgttttata ttgacgaagt tcgtgatgaa gttgattatg atgagttgcc
     1441 aaataatgaa ggaaaaattc gtattcgtac gaaaaactat gaagaaactg tgaagggagg
     1501 tgagaacgac aatgccgaaa gctaaaaaag taccatttca gtttactaac gaaattcaga
     1561 atggtaagca cattctcacc ttgagtggaa atgtacagaa aaaatattgg cgtgatgatg
     1621 atgtcataaa cgctaaagat attcgtgact ctctagatac tgtaaccgat gatattgtaa
     1681 tcaagttgaa cagcccaggc ggtgatgtgt ttgaagggat tgagatttac aattatttaa
     1741 aagatcaccc atcaaatatt actgtagaag ttacagggct tgccgcaagc gcagcaacgt
     1801 ttattgtcgc tggtgccaat gatgtgatta tgaatgttgg gacttctttg atgattcatg
     1861 aagcttctac atttgcttgg gggaataaac aggacatcca aaaaacgtta aacgcattgg
     1921 aaacgatcga tgattctatc ttagctattt attcagataa gactggccaa tctgttgata
     1981 agttgcgtga atggatgaat gaagaaaaat ggtttacagc agatgaagct gtcaaatatg
     2041 gatttgccaa ttctgtgaag cgtgaagaac cacaaaatga acctcaaaat attgcagcga
     2101 tgattcaaga tgctgttgcc gttgcaatag cgaatttaaa ggaacctgtt actaataaaa
     2161 tcgaacaaga atcaaaacaa aaatcattaa tcgcacgatt gcgaaaagga gaataaagat
     2221 atgttaaaaa ttacagataa aactgcagat gcaaaaaagg catttaatgc tatttcttcg
     2281 aaagaagatg caacacccga acaaattaat gctgctctag aagaatatgt aaccgccatt
     2341 gcagaagatg ccggcaaaca ggtgcgtgct gaatatgaag aattgaaaaa tgtgacggac
     2401 aaccgtgtat tagaggctcg tggaattcct acactaacag cagaagaaac aaagttttat
     2461 aatgaagccg taaaatctgg aggtttcgat tctgacgtag tttggccaga aacgattttg
     2521 gaacgtgttt ttgaaaatct tcaaaacgat catcctattt taggcctcat taactttaca
     2581 ccaactgtag gtcgtgtcaa agtaattaga gcacgtcgta aaggtgttgc tgtttttgga
     2641 ccattgcata aagaccttga aggccaacta gatgctgaat ttggtgcaac tgaatttgtt
     2701 caacttgcgt tgaccgcatt tttcttaatt tctaatgata ccttagattt aggaccacga
     2761 tggattgacc gttttatcaa tctttcttta agtgaagcag tacgtgatgt ttgggctaaa
     2821 aaaattatta caggttctgg taaagatgag ccaattggat tgcttaaaga tctagatggt
     2881 gcggtcacaa gtggtgtata tccagataaa gctagcgcag ggacattaac ttttgcgaaa
     2941 gagaagattg ttcttgaact tgcaggggtg atgaaaaaat tatctaaata cacatacaaa
     3001 gtagatggtg acgatgaagg tgaagtgaaa taccgttctg ttgctggacg agtgtatcta
     3061 attgttaatc ctgttgatta ctgggatatc gtagctagag ttacatttgc aaatttaaac
     3121 aatgtgtatg ggactacgat gccatttatc gctgttgatc atatcatcga atctgtagac
     3181 gtaccagaaa gtaaatgtat tgcatacgtt gataaagagt atgaagctac tcaatcaaga
     3241 gctgaaaaag tctatgtata taaagaaact tttgcaatga aacgcgcaac tttgtatgct
     3301 gtcgacatgc ttggtaatgg ctatccaaca aataatgatg cagcacaagt atatgacttg
     3361 gactttacag cgggggagta atgtcccctg caattggtga gataacaccg acgactgatg
     3421 gggcagtcat taatctttcg taaatggggt tagagtatga acactgaaga atttataaaa
     3481 gaatataagg cccgatttcg tattttccat acttccgaag atgaggatat cgggaaacag
     3541 ttagagagcg ggtttgccga tataaaatct ctaatcggca gttttgatcc ttccacttat
     3601 gagaaaggaa aagaattagt ttatgaacgt actcggtatc tacggaacga atcactagag
     3661 tacttttacg acaattttca aacaatgatt atggatgcat caattgatct ggcaggtgct
     3721 ggatatgcca attaaatcaa attataagaa gcctaaaatt gtagctggtg atctaaatac
     3781 gccagttact ttttttgagt ttcaacctaa taagggtcca gaacctggtg atgaagaaaa
     3841 aaaggaactg tatttttgta cagcactagt ttacaaccca tcaatgaaag atcgtgacat
     3901 tcttggcaca aaagaaggcg ttacgataaa aatacgggat ccccatcaat cttatattcc
     3961 tactaacaaa cataaagtaa ggatattaga ttatcgatat ggagataaag tttgggaaat
     4021 aatcgatgtc tcaccggatt tggaagaaaa ttcaattgta aagatagtat tagaattaat
     4081 tacttaaatt tggtgataaa tgatgagtaa cgtaaagttt gaaggcgttc aagaaacaat
     4141 ccaagcttta gaaaaaaagt atggagaaaa gaaaactaag acattaacaa aaaaagcaat
     4201 caatgttggc gctgaaaaag tagaaaaaca gttgcaaacg gacatgaccg tttttaaaga
     4261 caaaggttat acgatcgctg aggttgttcg caagaacgct acgtatagag attacaaggc
     4321 agaagctgaa atcggttgga atggtccaca tcaacgatat agaatcattc atttgaatga
     4381 atggggatat acacgaaagg gcaaacaaat tagaccgaga ggatttggtg taataacaaa
     4441 atctcttaag aattccgagc tgttgtactt tgatgctgta ggagaggagg ttaaaaagag
     4501 tttatgaaag atatgcttaa tatcatttac gaggctcttt gtttgaatga gtatattcat
     4561 tcaatgacct acaacgagaa taccgagcaa tatcgtatta aattttatga gcaacctgaa
     4621 accgccgata aaacaggtcc aatgatcaca attcgtcctg tagatacacc taacgaagcg
     4681 taccacggaa gtaacaaaga gctttctgtg gagcatatga tacagattga cgttgaatcg
     4741 aaatatagag caacatgcaa gcagattcaa tatgagataa aaaaagagat gaaaaaacta
     4801 ggctttggcc aaatttccgg tcaagggtta gatgaatact ttactgaaac aaaccgcttt
     4861 gtagacgcgc gtcgttacga aggaaataca aaaatttacg ataccgaata ttagaaatta
     4921 agacacgaaa ataacgtgtc tttttttatt acccaaaatt aggaggaaaa ttttatgact
     4981 ttagtaggat tcaaaaaaat gacaatcggg gtctttgaca gcacagggaa gattccagca
     5041 gcaaatttat atgtaattga aggtgaacaa aacaaagggg cgactgtatc tgctgaaatc
     5101 agtgggctat caaaagaacc atcaaaagtt tacggatcga acatcgctta ctatgtctct
     5161 caaaaaggaa caggcgatgt ttcagctact tttggattgt tagatttacc aacagaagta
     5221 aatgacaaaa ttcttggtta caaaaccgat acaaacaaaa tcagcttctt gggagaagat
     5281 actgagccac catattgtgc aattttgatg gaatcggagg atttgaacgg cgatacagca
     5341 atgcttgcga tgttcaaagg aaaattcagt cgtgaatcta ttaatcttaa cactacgaca
     5401 aatgaagctt tcgaaccaga agcagaagaa tatgtatttt cagcgattgc caatgatgtt
     5461 gaaggtgatg ctaaaggcca aacagtcgca aaatatgttg gagacgaaga agcttctatt
     5521 actggtttga aagagatggt atttcctgca gcgggggagt aactagcccc gttgttggag
     5581 caatcactcc cactattaat ggggcaacag ttgaattaag ttaggaggat aactatgcca
     5641 gatacattta aaatctataa aaaaaatggt actgaattta caaaagtcgc agagggtgaa
     5701 agtccacttt ctattactgg aattgcagca aatactcaag ttgctaaagg agattatcaa
     5761 gctacgcgtg tggtcggagg tcaagaatca gtaaaagtag atattcctgc attcaccact
     5821 cttccaattt cagttactgg tgtgacgttg gataaaacga cagctgaagt tgaacaaggc
     5881 ggaaccttaa aattaactcc tactgtaaca ccagcaaatg caacagacaa aacaggaagc
     5941 tggagcagtt cgaatacagc tgtagctacg gttactggag gcaacgttac agtaaaaaca
     6001 gacgctgaag ttggcggaac tacagaagtt tcatttacta ctacggatgg cggtaaagta
     6061 gcaaaatgta ctattacagt tattgaaaaa gcagcgggct agtcttcgga ctagcttttt
     6121 tatttggaaa ataatggagg aatctaaatg gcaaaagtaa gaattgaatt gaaagaagaa
     6181 tcaggaaaga aagtctatga aaacatagac acaacaggga aagattatcg aaaagcttta
     6241 gaaacaatca aaaagctaaa tgaagacgga gtaatgattt ggaatcgttt agacatttat
     6301 ttggattttg ctgtaggtat cttcagggat agcaaattga cagctgaaca aattcttgat
     6361 ggcttaccat ctgagaaggt aatgagcaca ttggacgaca tcctaggaga agtaatgggg
     6421 attgaaagta atccagaccc agacgcaaaa aagtaacacc cgaagaagca gaagaaatgt
     6481 acttaaacct atgcagagag ttagtgaaag cgggatggtc actatctgac atagaagata
     6541 attcctttga aatgatgatg aaaattgcgt gcacttcgcc taagaaagag aaacaaaaag
     6601 aagttgatct taaagacttc ttgaaatcca tgtagagagg aggtaaacat atggcaaatg
     6661 gaaaaccttt aggcaatatg attatcaaac tagacttaga cagttcagct ttttcaaaag
     6721 gtctagccgg agctaaaaac gctgttaacc atcaaatgaa agcaatgaaa tcacagatgc
     6781 aagtaatgaa ctcttcagga aattctctcg gtgctttaca agcaaaatac aatggtttag
     6841 gcggagttct acaagcaaat gaaaagcaag tagaattgct cactaaggct tataaggata
     6901 gttttgactc taacggaaac gctactgctt cgactgctaa atacgctaat caattgaatc
     6961 aagcaacagc tagatcagca agttacgaat ctcagatgaa aacaactgtt ggacagattg
     7021 ctcggatgaa agtggaaacg gaagggttta cggggaaact aaaggctaat tctgatgtat
     7081 tgattaattc tgggaaaaaa attgaagcct ttggtaaagg agtttctggg ataggatctg
     7141 ccctaactgt tggagtaaca gctccaatag tggcaggcgc aactgctgtt actagtgcag
     7201 ctatttcatg ggaaagtgct tttgcaggtg taaaaaagac aaatgatgaa gttgtagact
     7261 cgaatggaaa cgtagtttat tcctatagcg acttagagaa aggtctgcgt aatcttgcca
     7321 cccaattacc tggtagccat caagaaatag caaatgtagc cgaagcagcc gggcaattag
     7381 gtataaagac tgaaaacgta gttagcttca ctaaaacgat gatagacatg ggtgaatcga
     7441 ctaatatgtc ggctgaaact gcggcaactt ctttagcacg atttgctaat attacccaaa
     7501 tgtctcaaaa agactttgat aaattaggat cagtcattgt tgatctaggt aacaattttg
     7561 ctacaaccga atcagaaatc actgaaatgg gattgcggtt agcgggagct ggtaaacaga
     7621 ttggaatgag ccaaggcgaa attctaggat ttgctactgc tctatcttct gttggtgtcg
     7681 aagctgaagc gggtgggtct gccttttcga aggtcatgat ccagatgcaa ctagctgtag
     7741 aaaaaggatt tggcgctttt gatcagctga aacagatggc agaagaacaa ggtgttccgt
     7801 gggttaattt agtaaatgct gtgagagacg gtggaaaatc gttaaaagca gtatcagagc
     7861 aaatgggatt cacttcatct gatttaaaga aaatgtataa agaagcggac aattcgaaaa
     7921 gttcgcttga aaattttgcg aatgtggcag gtgttacaag tgaacaattc cagaagttgt
     7981 tcaagagtga tccttcagaa gcaatcatca agttcattca agggctgaaa gattcagaaa
     8041 aacatggtac ttcagcaatc aaagttctgg atgacatgga tataaaagaa gttcgactac
     8101 gcgacagttt attgagagct gcaaatgcta gtgacatatt caaaggagca attgaaaaag
     8161 gaaacggcgc gtggaaacaa aatacagctt taacaaatga agctaataaa agatacgaaa
     8221 caactgaatc aaagctaaag atattgaaaa acgaagtgat tgatacagca atcgatcttg
     8281 gaggtccatt tgtagacgcg ttgcgtgatg gtctaaatgt atcgaaacca ttgataaaaa
     8341 acttagggaa tatggcgaaa gctttctcag ctatgagtga tgatcaacaa agaaacatta
     8401 tcaaatgggt tggattagca gcagcagctg gtccagcgct gaagctttta ggaactggta
     8461 cttcagtaat aggtaaagtt acttcaacta ctggaagatt gacgaaaagc cttgttgatt
     8521 tagcagcgaa agcagcagaa aagaaggcta tggatgcctt tgcatcgtcg gtaacaacag
     8581 ctggtgctgc agctgcaaag acagcaggag ctggaggtat tggcgggtta gtccctatga
     8641 tgggaaaaac agctagtgct gcaagtactg ctgcgggcgc cagcggaata ggcgcaatga
     8701 caacgtcact aggtttgtta ggtcctgctc tacttggtat tgttggcgtt ggtggcgcac
     8761 tggcagttgg ctatggagca tggaaattat ttggtgaaga agcttggaat tcctcgcaaa
     8821 gagtaaagca atggggaaca gatgtcggtg aggagataga tagtactctt gacggagtac
     8881 agaagaatat tgaaggtgca aatggtcaat tcggtcttct caaagagggt tttactgttg
     8941 atgatgcaaa taatatggcg gctaatttcg caaccgctgg aacatctttg gaaacatctt
     9001 taacgggtag aataaaggca ttagacgatg cactaaaagg gctacctcaa tcagttcagg
     9061 atgccatgaa agaagtagct gaatcagaag aagaaacctt agggcaatct ctttctaaaa
     9121 taaaagacaa caatgaagaa ataaaaaaaa tcagagaaag agccgcaaat cagaacagag
     9181 acttgactgt ttcagaacta caacagatac aggatctaat gaaaggaact tctcaagcgt
     9241 atgtggatac gctcgatgtt tctgctaaag acagaaaaaa gatactatca gccatgaatg
     9301 gtgatgttgc acaagctaca gaagaagaag cgaagacgtg gttacaatct ttagcgaaac
     9361 aaaaagctgc aactaccgaa aattatagac aaatgcttga agaaaaaaag gcggctttaa
     9421 aggaaaaggg agtaagcaaa gaattcattg atgcattaga agatgaaatg aatgattaca
     9481 tcaaaactac aaatcaagga ttcgatagcc aaatagctgc tataacaaat aaatatccac
     9541 aattgattaa cgaagtgagt ttatcaaatg gtcaattatt tagcaaaacg gcagctgcta
     9601 tggacaatac tggtcagtat acaaaagttt ttctcgaaag taatgaaaag attttagccc
     9661 aagcgaagaa aacatcggat gaaatagcag ataatgccga aaaaaatgca gaacaacttg
     9721 gtatggtagc tgatgaggct acgcatgctg gatcaacttg gaatggctta gttttagacg
     9781 aaaaaacagg aaaagtaaaa actaatgtac aagaagtagt gaatgaagca acgaaggatt
     9841 tcactaaatg gaatgaattg aaacctgttg ttcatgatgc gaaactaaca acgaatgcaa
     9901 aagatgtcat ttccatagcg gcagtacaaa atggctattg ggatgatatg gaatggaaag
     9961 aaaaagaagc attattagaa gatgagtgtt ctaaaaatgt atacaaggct ttagaaaatg
    10021 ctggtaaatg gaatgagtta gatattcctt ctaaagaagc aatcctaact tcaaatactc
    10081 ctgaagttat gggtgaaact ctcatgaatt tgggattatg ggatacttac aaacttcaag
    10141 tgaaagattt agatttgaat gcgtataaag tatacgatgc tatcgatgga tcagaagaaa
    10201 aattaaatag ttgggctcaa ttagataacc ccacaaaaga attgttatta gataactcag
    10261 atttttctac aaaacttttt aattccgaag aaatgtttaa acgttggaat cagttatcat
    10321 ctaatgcaaa gtttctttcg gctaataata ctaaatttac agaggttctt ttatcatctg
    10381 acagaatgtg gaatcaatgg gtaaaccttc ctgataatga gaaaagatta cttgcagata
    10441 acgaagattt agctaccact gttttatctt cagaagaaat atacggaaga tggactcaat
    10501 taccagataa tcaaaaatat atgttagctg acaatactga cttagcaaac aaaatattcg
    10561 cttcagaaga atcatggaat gcatggaaag caattccaga cacagttaaa aacttgcttg
    10621 gtaataatat tgatttactg gctaaagtga aagacgggac tgtaagtgta gaagaatata
    10681 acaaagttat gccacttctt aaaactctat atggagataa cgttaattta aaagaggcaa
    10741 tcagtggggc taaaagctct atagagaatt acaacaaaaa tatttttcct agtgataaaa
    10801 ctgcagtagg acacgatgag gcttcacaag ctgctaaaga tgcatttgaa gcgttcaaca
    10861 tcttccaaac aaaaatacca gataaaatta ctaaaactgt atcagctgac tttataggtc
    10921 ctatgccgaa aaatgcgaaa ggaacaaact tccatcctgg cggagcagct atggtaaacg
    10981 atcaaaaagg gcctacttat gaagagctta taaccttgcc aagcggagaa acttttatac
    11041 ctaaaggacg taatgtcgtt ttagacttac caagaggttc gaaagtactg aacgccacta
    11101 aaactaaacg tctagtgcct aaatatgctg atggtatagg aaacataacg acggtttcat
    11161 ctacgctaaa cttagatgct ttaattttag ctattaacga gctaacgacg gtattgagaa
    11221 ctcaactgat ggcaggaacg actgatgcta aagatactaa actatcagat gaaacaaaag
    11281 ttgccgatcc gattattccg gacagtcttt ctgaaaaatc agatcagtat ctaggaattg
    11341 gagctcaatg gttaactaat ctaatgaatg gttggaactc agcggttcct caatatatga
    11401 atagtgaaat ggtttttatt acaaactatc tcaatgcgct aaaacttcaa aacaatccta
    11461 attatacgca aggagcaaca tggaataaga acttactgaa tggatggaat agtttaactg
    11521 ggacctttat tgctacaatc aattcatttt gtaatcaagc aatggtcacg cttagaaact
    11581 acaacactcc tatgtacaac aatggccgaa cgtggcagca aaataatctt aatggttgga
    11641 attcattgta tggatcattc atagcccgtg taaatcagct tggtaacgat tcgattaaca
    11701 accttcgttc taaaagtggt ggtttttaca atgcagggac attcttgttg caatctttaa
    11761 taaacggaat gaattcgtta ggtaattctc tttctactac aatgaacaat gtcgctaata
    11821 caatggttgg tggtataggc aaaggagtta atggcgtcat atcgggagtt aactatgtgc
    11881 ttaaggaagt cgaatcaagt aagaatatcg gaaactggcc aattccacaa tacgctaagg
    11941 gaactgatgg acatcctggt ggacttgcta tgattaatga tcaaaaaggc ctagttcacg
    12001 aagaatatgt tcagatgcca gatggacgta gttttattgc caaaggtaga gatcttcttg
    12061 ttaatttgcc taaaggtgct caggtcttaa atgcttcttt aacaaagaaa ttaaaggaac
    12121 gtttaaatat tccacactac gaaaaaggta ttggcaattt agatattgtt gatttgttag
    12181 atgatgaaaa aagaatgtta gaattcttaa ctagcaaagt tgatttttca ggcattaatg
    12241 aaccatggct tgatatgact aaatcaggaa cgtcattaat gtccaaagct gcaaatacaa
    12301 tgcttcaatc aaaattaagt gaattcttca ctcatggcaa ttttgatggt gcagtaaatg
    12361 ccaatggtgt ttatcaatat ttagttgatg tggcacagaa agtaatgggt aagttccccg
    12421 gacttacagt aacttctgga tatcgagcag gagatgccta ctatcacgga aaacggcaag
    12481 ctatcgactt ggcttatcca ggtatttctg gagatccgag atatacagca gcagcaaatt
    12541 acgcttttga aaaattccct tcaaaaattg cgtatgtcat tacgaatgga cgtgtacgtg
    12601 accgcatggg attatctgga acaaggtcaa gcggacaatg gacgaattgg ccagatggcg
    12661 atcacttcga tcatattcat ttaaacggtt cgatggggtc tggcgatata ttcaatggag
    12721 gagttggagg aagtggtgtt gcacgctggc gttcttatgt gtctaaagca ttgaaaatga
    12781 atggattacc agctacagca gcgtatataa atgcctggat gtcccaaatc caaacggaat
    12841 ctggtggtaa tgagaaagca atcggcggca atgatgggct cgccgaaggc aatgcaacag
    12901 gattacttca aacgaaaccc ggaacttttg cagctaacgc ttttcctggt cacggaaata
    12961 ttatgaacgg atttgataac atgttagcag caatcaatta tgctaagaaa cgttacggag
    13021 ttgcaggtat gttgcaagtt atcgggaaag gacatggata tgccaatggc ggcttgatta
    13081 ccaaagacgg actatatcga gcaggagaag gaaataaacc agaaatggtt attccattga
    13141 caagaaaaac aagagctatc gaacttatgg gtcaagcatt agccttcctt tctggaagca
    13201 ataaaaaaga aacgaaaagt gagattaatt ctgtagataa tagacatatc ctacaagcga
    13261 tagaaaaaca gtctcgtatg acaaatgatc tgctatcatt attactctcg ttttttaaaa
    13321 gtaacaatcg atcagataaa gaactagctt tagatattca gaaaatttta atcaggagga
    13381 catagtattt gaggacagta atattaagaa ataaattgaa tgaagaaatt gatttgtcca
    13441 cagaagatta ttttgctact gaaatgtcga atctaggttt tgaagttaca aaagagcata
    13501 ttggacaatg ggggagtttc agagaaatag gagagagtat tgaattgtct gaatttcaat
    13561 catcagttat gatttctgtt cacggattta gagaacaaca attatatggt tcattagttg
    13621 aatttctggc taatggacct tttgaactag aattttcttt tgattcagaa acaatgatca
    13681 ggaaatgtag tttgaaatca ttatctaaaa cagaaattga ttcccaaagc tcattgctaa
    13741 cagatacttt gaatctatat tttacttcaa gctggtattc tattaagcgt gaaaaattaa
    13801 tacagaattc aacttctctc aatacacgtg gaaaagttta ctcatttgtt cgaccatatg
    13861 tctacaccaa aaatgtatgg gaaaagaaag gtgtatttaa gttcagtaat aattctgtat
    13921 atttgacaaa tagtacagag cgaatgtctc cgttaaaaat aaggattatt ggagagtgct
    13981 caaatccata ttgggaagtt atccaaaact ctaaagtagt agcttcagat ggctatttta
    14041 ttaatatgaa agacacacaa atattagaag tgtctagttt gctagaagac caaacggcaa
    14101 ttttaaaaga tgtaggtgga gttgaatctt ctgtttatca acaacaagat tttactaaat
    14161 caaacttcgt ccaagcgcct ctaggagaat tcagcgttgt ttttcatgtt ggacatgcaa
    14221 atgtagagat tgaactttat gaggagcgtg atctattttg attttagcca ttaccctctt
    14281 tcaccgagat atgaatttat ataacgaacg tttaatttcg actgagttcg aatttggaac
    14341 ggatgaaatt ggagaagaag taagtaattt tattacagat aaatatgtcc atgtaaaatc
    14401 aggagatttt ctactagcaa aatttattcc tagcgggaag attgcttatt ttggagtgat
    14461 tagctcgcaa gaagacaaaa aaataaattg caaaggatta ctcagtttgg ccgatagcga
    14521 gataccgact gtaaaaatat caggtgctaa ttatgaagag catattcgac gactgattga
    14581 atactactta ttgaacgatc caacaaaaca actaaaaaat attttggagg taaaaacaga
    14641 aagcgttact tctcattctt atcaagctgt tgacacaaat aagagacgat tgaatgcata
    14701 tataataaat gggttcaaaa aatataatgt taaatggtat gtcaaagaga ttaaaggtaa
    14761 aaaaatatat actggtattc gagcagttga tgattcaatt tatattaaag ataattcatc
    14821 tgagtttaat gactgggatg tttttgtgca atctcctgga ccagggaacg agaacaaatt
    14881 attgatcgta gataaagcaa tgaaagatat agaaaatccc ttggtattgt ctacgtggta
    14941 tttagatgac gaaaataatc ttacgcaaga ttcaacgaat aagaacattg cattgcctac
    15001 cgttaattta gtaaatatat atgatcaaac tcaagaagat agaccttctt atgaagaggt
    15061 agctaaatcg gagcttaaag gcaacgccta ttcacatgaa attaaagtta atgttgtgct
    15121 aaatgctaga aatatcaatg tagaaaatat cgaaacagga atgttagcga ctattgttta
    15181 caaagagcag acttacaaat ctgttctaac tgcctggcga atatccagca gtaaaaagaa
    15241 tatagaattg acatttggta atgtccgaag tcgttttatg gattattttg aagataatgg
    15301 gggataaaaa atggttagca atgtggatgg atatcaattt gaaaacgtga aagtaagcgc
    15361 agaaaatgat gctagacttt atcacgtttt atataatcgg aaaaatcagg ttattgatgg
    15421 ttacgatcag tctatgaatt tatcttcaag cggattaaca gtaaaagttg ctgcaggagc
    15481 agcgattatt caaggtcgta tggtcgttgt tcgacaagaa gaaagtataa cagttccagc
    15541 aaactcaagt ggttatatag cattaacagt ggatttgaca caagaagtta tacctggatc
    15601 tattcttcca gaatcggaag aatatgaatg gactaataat caagtcaagc tagaatttat
    15661 aacaaaagtt ataaaaggta atttaaacaa tggtgataag gtctataatt taccactatg
    15721 ctcagttaat tctactggat caactgtttc aatctcaaag atatcggata gttacgagct
    15781 gactctctct aaaggagaaa ttttgtggag ggggactgcg ttaatgcatg atactcaaac
    15841 tgtccaacct tcaaaaaaaa tttggcagac agtttcaggt tttttgttag tatggctccc
    15901 atacgaaaac gggcaagcaa ttgaggatag atatgtaact acgccttttt ataaggagcg
    15961 tataactttt actaatgttt taggagaaat tgtttcagga tttgatgact atcacaaaaa
    16021 gtggtttagt aaacgaataa actataattc gaatactaat atatttacag gtgcagcaag
    16081 caatgcaagt ggagataacg caaatatggt gcttaggtat atagtttctt tttagttagg
    16141 aggtatggaa gagtggcaaa tttagaaatt aaattatctg caaataagag acagccttat
    16201 ctacgtcacc gtgttgttgg tagagttggt gatggggggc tcacaacaat caatgtacaa
    16261 cttcttgagg aagatgaaat tacacctttt gtaattaatc taaacggtac tttgaaattt
    16321 gtgggcgaag tttcaaacgg taactatacc gagggagaac cagaaataat cgattcgact
    16381 aatgggttaa ttagttacac gttcactaag tcaaatttca gcacgagtca tcaattcaaa
    16441 caagcatatt ttgaatatgt agatcctaac ggcaaaaaag taacttttca gaacttcatc
    16501 atagacgtgt tagaacgagt agatattaat tcggagcaag cgaaatacta tatttcttca
    16561 ttggaaaaat tacagagtga aatgcaaacc actttcaatc agttcattag tgataaacag
    16621 gtccaatacg atcaaatcta ctcgaaatat aacgaattag taagattgat aaatgaatca
    16681 gataagcaag taaatgatcg tattgataag gcaaataatc gtattgatca aaccaatcag
    16741 caaatcggcg atctcggcaa gctgaaaaag atgtacagta acagcattga
//
LOCUS       DAZKBH010000056        16638 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000056 DAZKBH010000000
VERSION     DAZKBH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.056"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            267..2738
                     /locus_tag="ACG8OO_001711"
     CDS             267..2738
                     /locus_tag="ACG8OO_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9045589.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2841..4577)
                     /locus_tag="ACG8OO_001712"
     CDS             complement(2841..4577)
                     /locus_tag="ACG8OO_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9045590.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4574..5278)
                     /locus_tag="ACG8OO_001713"
     CDS             complement(4574..5278)
                     /locus_tag="ACG8OO_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9045591.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5409..5912
                     /locus_tag="ACG8OO_001714"
     CDS             5409..5912
                     /locus_tag="ACG8OO_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFP9045592.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5872..6210
                     /locus_tag="ACG8OO_001715"
     CDS             5872..6210
                     /locus_tag="ACG8OO_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFP9045593.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6231..7649
                     /gene="ffh"
                     /locus_tag="ACG8OO_001716"
     CDS             6231..7649
                     /gene="ffh"
                     /locus_tag="ACG8OO_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9045594.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7761..8339
                     /locus_tag="ACG8OO_001717"
     CDS             7761..8339
                     /locus_tag="ACG8OO_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9045595.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8343..8864
                     /locus_tag="ACG8OO_001718"
     CDS             8343..8864
                     /locus_tag="ACG8OO_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFP9045596.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8943..9497)
                     /locus_tag="ACG8OO_001719"
     CDS             complement(8943..9497)
                     /locus_tag="ACG8OO_001719"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9045597.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9750..10025
                     /gene="rpsP"
                     /locus_tag="ACG8OO_001720"
     CDS             9750..10025
                     /gene="rpsP"
                     /locus_tag="ACG8OO_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9045598.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10037..10288
                     /locus_tag="ACG8OO_001721"
     CDS             10037..10288
                     /locus_tag="ACG8OO_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFP9045599.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10413..11204
                     /locus_tag="ACG8OO_001722"
     CDS             10413..11204
                     /locus_tag="ACG8OO_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045600.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11374..11895
                     /gene="rimM"
                     /locus_tag="ACG8OO_001723"
     CDS             11374..11895
                     /gene="rimM"
                     /locus_tag="ACG8OO_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9045601.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11885..12646
                     /gene="trmD"
                     /locus_tag="ACG8OO_001724"
     CDS             11885..12646
                     /gene="trmD"
                     /locus_tag="ACG8OO_001724"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9045602.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12782..13129
                     /gene="rplS"
                     /locus_tag="ACG8OO_001725"
     CDS             12782..13129
                     /gene="rplS"
                     /locus_tag="ACG8OO_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9045603.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13261..14397)
                     /locus_tag="ACG8OO_001726"
     CDS             complement(13261..14397)
                     /locus_tag="ACG8OO_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9045604.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14513..15427)
                     /locus_tag="ACG8OO_001727"
     CDS             complement(14513..15427)
                     /locus_tag="ACG8OO_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFP9045605.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15518..15946)
                     /locus_tag="ACG8OO_001728"
     CDS             complement(15518..15946)
                     /locus_tag="ACG8OO_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9045606.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15951..16325)
                     /locus_tag="ACG8OO_001729"
     CDS             complement(15951..16325)
                     /locus_tag="ACG8OO_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9045607.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         5370 a         2986 c         3259 g         5023 t
ORIGIN      
        1 actcctttgg taggatgacg ttttggtcga ctaaatttta ccaaaagtga gtctcttttt
       61 gagttgaaac gaagaaaaag tgttagtaga aaatcgcctt cgattttcca ccaacacaaa
      121 atattataga accctttttt tttgctatat gacaggaatg aaaggtgttt gcaagcttaa
      181 tgtgaggaac actttccata tctgttctat actggatagg agaagtttgg aaatatccag
      241 aaaacgaaat caatcgaggt agccacatga agaataaatg gataaatcga agtaaaaaaa
      301 agaaagtaac acaaagatca tcagataaag tcatccgtct ttttccatcg tataaagaag
      361 gattaacggc agatcaagtc aaggaacgaa tagaaaaagg tgcagcaaat aattcagtcg
      421 atcccacatt caaaacaaat cagcaaatcg tattggaaaa catatttacg tatttcaatt
      481 tgatctttct tatcttagcc attttgcttt gtttagtaga atcctataag aacttgactt
      541 ttttaccagt tatcatagcc aatacaggaa tcgccatttt tcaggaaatt cgatccaaaa
      601 agatcttgga cgaactaaat gttttaaata cagcaaaagt caatgtcata cgagacggga
      661 tagaacaaac aatagatata gaagaccttg taatagatga cattgttatt ttagagactg
      721 gatatcaaat tccagcagat gcagaggtcg tggaagggaa actgcaagtt aatgaggctt
      781 tattgacagg cgaagcagat gaaataacca aagaaatagg tgatccatta atgtccggca
      841 gtttcattgt ttccggaaaa gcttacgccc gattagacaa agttggagca gattcgtata
      901 tttcccagct gacagtcaaa gccaagacaa tgggagaagg agaacagtcg gagatgattg
      961 cttctcttaa tcaattgatc aaatggattg ggatcatcat tattcctatc ggtatcctgc
     1021 ttttttctca aagttacttt tttaatggaa acacgatcaa agaaagtgtc gttgcaatgg
     1081 aagcagcatt gatcggtatg atacctgaag gactgtactt gctgacgacg attgcattag
     1141 cattaagtgc gactaaacta gcaaagcaaa gagttctttt acacaatatg aaaagcattg
     1201 agacattggc aagggtaaat gtcctttgtg tagataaaac aggaacgatc acagaaaata
     1261 aaatgtctgt tcaaaaaatg atcgtctcta aaaaacaaga aataatcagt cagtcagatc
     1321 aattggaagc atgcattgct gactatgcaa aagcgatggc aggagacaat gcaacaatgg
     1381 aagctgttaa agcatatttt acaaaaacca ctgatcaatc ctatcaccat gttattcctt
     1441 tttcttctgt ccaaaaattc agcagtgtag cgttggcaga caaggtgtat gttttaggag
     1501 cgcccgaaat ggttttgcgc gatcaattgc cggaatatgc ggaagagttt gtttctttcg
     1561 ctgaaaaagg ctatcgcgtg ttggtctttg gtgtgtacga tgggattctt gaagaaccag
     1621 tattgacaga agcagtcaca ccattaggct atattctgat tgctaatcct atcagaaaag
     1681 aagcaagagc cacatttgat tacttcaaaa aacagcaagt agccatcaaa gtgatttctg
     1741 gagataatcc gctcaccgta tccaatgtag ctgtccaagc agggatttca catgctgatc
     1801 agtacgttga tgcatctaaa ttatcagaag aagaatatga agcagctgtc gaaaaatata
     1861 cagtttttgg acgcgtcaag ccagaacaaa agaaaatctt cgttcaattg ataaagcgta
     1921 acaatacagt agctatgaca ggtgatggcg tgaatgatat tctagccatg aaagaagcag
     1981 actgcagtat agcgatggct tccggaaatg aagccgccat gcaggcctca caagtggtct
     2041 tacttgattc tgacttttca cgcatgccag aagttgttgc agaaggacgg cgcgttgtga
     2101 acaatattga gcgatcagcc agcttgtttt tagtgaagaa tattttctcc tttttgctct
     2161 cactgttttc agtgatcttt gctctgacct atcctttaga accatcacaa atcacattga
     2221 ttagtttgtt tactatcgga cttccatctt tcttgcttgc tctagaagaa aacaaaaagc
     2281 gaatcagagg gaaattcatc atgaatgtga tggaaaaagc tgtgccaggt ggactaacag
     2341 atatgatcgt agtcggtgcg ttggtgatct gcggggtgac tttgaatcta aataaaacag
     2401 atgtttctac agcatctacc atgcttttga ttgcagtagg gttcctcgtt ctctataaaa
     2461 tctgttcacc gctgaataaa ttcagaagtc agatcattct tttctgtgct agcggtaact
     2521 ttttttcggt catctttttg cataagctgt tttcaatcac cggtatctca gctgtatcac
     2581 ttttgctgtt gagcattcta tttttctcgg cagattcgat ttttaggcat ttgaccacat
     2641 tggtagaata tttgtttgaa cgtaaaaatg aaaaagagca aaagccgttt agtcgcgtag
     2701 ctaaaaaatt tctatcgata tttaaacgag aaaattgacg aaaagtatct caaaaaagag
     2761 taaaaaagaa caaccactcg tgtatctttc gaagcaggat gagtcatcca actttaaaac
     2821 gcacgagcag ttgctctttt ttacattttc ggaatggtaa cggtgaaagt cgaacctgtt
     2881 ccaagatgac tgtctacagt aatcgtacca ttaaggagtt gcacgtagtc ttttacaatc
     2941 gctaacccta atccggtccc gcctgaatgt cggcttcgcg ccttgtctac gcggtaaaaa
     3001 cgctcaaaga tccgttcctg atcctcaatg tcgataccta taccaaagtc ttggacagaa
     3061 aacgatagat catctgttgc ctgatagcgg ataataatct cgctgtcgct ttttgaatat
     3121 tggacagcat tttcaattag atttttgata attggataaa ggatatctgt tttagtagta
     3181 aatggttcgt tctttccaat caatcgaatc gtcaaccgtt tcttttcgat gattggctga
     3241 taattttgaa cgatttgctg gaaataaggt tctgtataaa ttgtctggat ctcgtaagaa
     3301 ttctcgccat cttttgacag ctggatgatt tcccgaatca gtttatctaa ccggatcgca
     3361 tctttttgca tgatctcaag aaaagaagtc agagtttgcg ggtcatcttt tgcaccatcc
     3421 agtaaagtct cggtaaaacc aatcagtgaa gtcacaggag tttttaattc gtgagagacg
     3481 ttgccaacga aatccttttg tagcttttct aaacgtcgca ctttcgttaa gtcatacgcc
     3541 acccctagaa tctgtccggt accttctgaa ttagtaaagt agcgcaaact caagtctaat
     3601 gtctgccgac cttctgtaag ggtgatctcc tgatggatca gcggatgctc aggagtcact
     3661 tgatgaatca gctgaatgag ttttggctct tgaatgactt ctgtgtaccg ctgttcaggt
     3721 tgatagctta ccacgtttaa atgtagctgc atctttggat taagcagtag taaacgagaa
     3781 tcagcatcat caatcaggaa tacgccaatc atcagttcgt tcagtaacgt atataattgc
     3841 tcttctgtgg tagtacacgc ccgatacatc ttactcattt gttcgctcaa cgcattaatc
     3901 gtctgataca attcttccca ttgatcgggt gtttgcatga ttacttccgc ctgattagga
     3961 ttttgcacca ttttttttag tacgggcagc acggttttca atggctggtt tttttgatag
     4021 atcagataat agatcattcc tgcaagaaca aggaagaaag acagaaagaa gaaaaagacc
     4081 caacggcgaa aactctcagt tctcggtaaa aaaccgcttg tcggttctgc gatacgtaaa
     4141 atggcttcta actggccact ttttttgact ggtaatgcga catataaaag atcctcattc
     4201 aaagtagtac ttttgcgcaa agcgctacct aggttcccac cattcaatac ggctttgatt
     4261 tcgggacggg agtttcgctg ttcattcaat gtttcattgt ttgtgtcaaa taagatcttt
     4321 ccagtagcat ccattaaagt aatccgttcg ttcgactgat ggacgaattc ttccaaagca
     4381 gttttgtttt gcgctgcttg gaggttttcg acatctaatt gccggatcag cagttgtgct
     4441 ttttttgtca ggtagctttc ttgctgttcc acgatctgct gttgaaaata atgggaaatc
     4501 aattgccagc caccaaaaaa caaactaact aaaagaataa gaaagaagag acgttcataa
     4561 atttttttcc atttcatcgt ttgggttcct gaaacttata gccaaatcct ctaatagtca
     4621 ccaagtactt tgggtgtttt ggatctcgct cgattttttc tcgcaaatga ctaacgtgta
     4681 catctacgat tcggctttgt cctgcgaagt caaaattcca aatgcggtct agcaatgtgt
     4741 ctcggtcgat cacacggtct tttcgtttca tgaaataaac aagtaattca aattctttcg
     4801 gtgttagttc aatcagatgg tcgtctacgg tcacttgata attggtcaaa tcagcagtga
     4861 tttgtccaat cgatagatat tcaggctcag cttcatcttt tgattgctta cgaggttcaa
     4921 ttcgtctgaa tattgctttc atacgggcta atacttcgcg aggactaaat ggtttggtca
     4981 aatagtcgtc agcacctatt tctaggccaa tgatccgatc cacttggtca tctttagccg
     5041 tcagcatcaa aatcggtgtt tcattttttt cttgacgcag ccgctgagtg atctccatgc
     5101 catccatcgt tggaagcatc acatctaaaa tgatgaaatc aaatgattgt tctaatgcta
     5161 attctaatcc ttcgcctcca tctgtggcac tggttacctt gtagccttct ttttccaaat
     5221 tgaacgtcaa gagcgttaca atagaaggtt catcatcaac aactagtatt tttttcattc
     5281 aaatctcctt taaataggtt tcattctgtt tatattttag catagaatca gtacaaaggg
     5341 ctattttaca aaagaaataa atggtataat aggaaggcac tttgctgaat agaaaggaag
     5401 agcagtcaat gattggaatc agtgcatgtt taggcggtct ggcctgtcgc tatgatggac
     5461 agaaaaaaga agtaccagaa ttgatggaac tagttgaaac tggagaagct gtcatggtct
     5521 gtccagaagt gatcggcgga ttaccaattc ctagagatcc cgctgaaatc attggtggaa
     5581 atggattcga tgtgtggaaa gatcaagcaa aagtatggac gacaagcggc gaagatgtaa
     5641 cggaagcgta caaaaatggc gcgatcaaag cctatcaaaa attgcaggaa aaacacattg
     5701 atctagtcat catgaaggaa aaaagccctt catgcggaac ccattcgatt tatgacggaa
     5761 gtttttccgg aataaaaaaa aatggtgccg gagtggctac tgcttatttt attcagcaaa
     5821 aaatgactgt gctctctgat gaagaatggc tgaaaactag aggtgagcag aatggaaatc
     5881 gaaaagacca atcgaatgaa cgcgctcttt gaattttatt cgaccttatt gacagaaaaa
     5941 caaatgaact acatggaatt atattatgca gatgattttt cattaggtga gatcgcggaa
     6001 gaatacgaag taagccgaca agcagtatat gacaatatca agcggacaag caagatcttg
     6061 gaggattatg aaaaaaaact ccatcttttt tctaattata ttgttcgaga acaggtattg
     6121 gaaaatatga ctgcatatat tacagataag tacccggaag ataaaaaact acttgaatat
     6181 gtacagcaaa ttcaagcaat cgaagaataa ataaaaggaa tgaagaaaat atggcatttg
     6241 aaagcttaac tgaacgtctc cagcaggcga tgggaaaaat aagaaaaaaa ggaaaagtat
     6301 cagaagctga cgtaaaagaa atgatgcggg aaatccgcct tgccttacta gaagccgatg
     6361 ttaacttgca agtagtcaaa gattttacta agcgcgtacg cgaacgtgcg gtaggggcag
     6421 aagtgctgga aagtttatca cctgcacaac aaatcgtaaa aatcgttgat gaagaattga
     6481 cgatcacact tggttctgaa acagcagagt taaataaatc accaaaaata ccaactgtta
     6541 tcatgatggc tggtcttcaa ggggcaggta aaacgacctt tactggtaaa ttagccaatt
     6601 atttaaagaa aaatgaaaat gctcgtccgc tcctaatcgc aggggacgta tatcgaccag
     6661 ctgcgatcga ccaattgaaa gtattaggtc aacagctaga cgttcctgta tttgatatgg
     6721 gaactgaagt cagcccggta gagatcgtcc gtcaagggat ggagttagca aaagaaaaga
     6781 aaaacgacta tgtattgatc gatacggcag gacgtctgca tatcgatgaa acattgatgg
     6841 atgagttgaa acaaatcaaa gaattgacgc aaccaaatga gattttgctt gttgtcgatg
     6901 ccatgactgg tcaagatgcc gtcaacgtag ctgatagctt caaccagcaa ttaggtatta
     6961 caggggtcgt catcactaaa cttgatgggg atactcgagg cggggcagca ctatcgatcc
     7021 gctcagtgac aggcgcacca atcaaattca tcggttctgg tgaaaaattg actgacttag
     7081 aagtattcca cccagatcgt atggctagcc gtatccttgg tatgggggat atgctgacat
     7141 tgattgaaaa agcgcagcaa gactacgacg agaaaaaagc ggaagaactt gcacagaaaa
     7201 tgcgggaaaa ttcattcgat ttcaatgact ttattgaaca gcttgatcaa gtgatgggta
     7261 tgggcccact ggaagatcta atcaaaatga tcccaggaat gaatcaagtc cctggaatcg
     7321 aaaacgtcaa ggtagatcca aaagatgttg aacgcaaaaa agcgatggtt tattctatga
     7381 cgccagcaga aagagcaaat cctgatctgt tgaacccaag ccgtcgtcgc cgaattgctg
     7441 ccggttctgg aaacagtgtt gttgaagtga atcgaatgat caaacaattc aaggaatcaa
     7501 gaaaaatgat gcagcaaatg tccaaagggg atatgaacat cccaggtatg gaccaaatgt
     7561 ttggttcagg cgtcaaagga aaactaggca aaatggcgat gaatcgaatg atcaagaaaa
     7621 ataagaagaa aaagaaaaaa cggaaataag atgaaaaaaa cagcttcggt acagactgaa
     7681 aatgtctact gccggagctt ttctatcagc aatttttatt ctttactaat acaattactc
     7741 taaacgaaga ggtggcacag atgagatgtc cgtggagtga acaaacagaa agtatgcggc
     7801 aatatcatga tactgtctgg ggtgttcctg aatatgatga ccaaaagctg ttccgaaaat
     7861 tgatgcttga tattaatcaa gccggattga gctggcagac gattttaaat aaaagtgaag
     7921 cattcgatga agcatatgat cgattcgata tcaaaaaagt cgcaaattat ggagaaacaa
     7981 aaatcagtga actaatggaa aacaaaggaa ttattcgtaa ccgaagaaaa atcgaagcag
     8041 ccgtccaaaa cgccaaagca gtcttgaaaa ttcaagaaga aatcggcagt ttttcagagt
     8101 atctttggtc gttttctaat ggaaaaatcg ttgattcttc ttatcattca caagaagaag
     8161 tcccaacaac aagtcctctt tcagatgaat tagcaaaaga tctaaagaaa agaggattta
     8221 aatttatcgg aagcacaaca atctatgctt ttttagaagc tgtcgggatc atcaatgatc
     8281 accttgacag ttgtttccgc aaagaagagg tcgtacgtat cggcagaaag ggcgaataaa
     8341 gcatgcgaaa aatcatattt tatggtgcta taagtttaga tggctatttg gcaacgaaaa
     8401 cggatgatct tcaatggctt tttgacactc caacaggtaa gcaaactact tatgaagctt
     8461 tttatgaaac aatcgatacg accatcatgg gcagaaaaac ttatcaggaa gcaaaaaaat
     8521 atttggagac cgaagcaatt tatccagaga aaaagaattt cgttttttcc acccacgcac
     8581 aattacaatt gccagatgct gaggtcgtaa acgaagatcc tgttgctttt ctaaaaaatc
     8641 tacaaatgac agaaggaaaa gatatctggg ttgtgggtgg cggtcgattg atcaaaccgc
     8701 tattggaaca tcaaatgatt gatgaatggt atatccagat tacccctgta ttattagggg
     8761 acgggattcc tttattccaa aaaggaacat atgaagcgcg ttttgaaatg ctagacacga
     8821 cacgattcgg tgaatttatc gaattgcatt atgtacgaaa gtagaagtaa ataaaataaa
     8881 aaaggcaact atatgcagaa atgtcctgta catagttgcc ttttttatta ccgttatgct
     8941 ttttactctg ttaatttgtt agaaaaatct agatacgcat ctaaaatcgt ttggaagaaa
     9001 cttaatgttc cttcaacgat attaccattt tcatcaatta gattcgcaac gtttccgata
     9061 tatgcttccg gttgctgcaa ggttggcaca ttcaaaaaga caagtgattg gcgtagatgg
     9121 tggtttgcgc caaagccgcc gatcccacct ggtgatgcag taacgactaa acctggttta
     9181 tgatcccaaa cactgtgtcc ataaggtctt gagcctacat ctaaagcatt tttcaatact
     9241 gctggcacag aacggttgta ttcaggagta acgaaaacga caccgtctag tcccctcact
     9301 tcctcacgaa aacgagtcca ttcagcagga gcctgttctg gagtttcgag atcctcattg
     9361 taaaatggca gatcatcgat ttttacaaat acactttcgt acccttcagg aagcaatttc
     9421 gcaaaagctt ctgctacttt tttgttgtaa gaatcttttc ttaaactacc aataaaaaat
     9481 ccaatttttt ttgtcatacg atttttcctc cttcaaacta tctataataa tcttacaaaa
     9541 gataagtaac tggagcaaat aaaaacactt actgacaaaa gctacataga agatagatta
     9601 cttattttta acaaaaaaga agtctaaaat cttctatttt ctaaaaaaaa tcgaatttaa
     9661 tcaagctgta aagaaaaaaa gctttacagg gtaaaaaaaa actgttacac tacatcttgt
     9721 gaataaaaca atggaggtgg aaataaataa tggcagttaa aatccgttta aaacgtatgg
     9781 gttctaaaaa gagtccgttt taccgtatcg tcgtagctga ttcacgttct cctcgtgatg
     9841 gacgtttcat cgaaactgta ggtacttaca atcctttgaa agaccctgca gaagtagttt
     9901 taaaagaaga cttagttctt gactggttat caaaaggtgc acaaccttct gacactgtac
     9961 gtaacatcct ttcaaaagaa ggcgttatgc aaaaacacca cgaagctaaa ttctcaaaga
    10021 aataaggtga ccggatatga aagatttaag cgacttagtc ttaacaatcg ttcgtccgct
    10081 agtcacttat cctgatcaag tcaagttaga cgtcgtagag tcgaaagact tttacgagta
    10141 caatctaaca gtggctcctg aagatattgg ccgtatcatt ggaaaacaag ggcgtgttgc
    10201 taaagcaatc cgcacaatcg tttacggtgt tcggatcaat gcaccgaaaa aagtgcgttt
    10261 gaatatcatc gatgataagg aaaagtaaat aaacgtgctg tactcgaatt ttcgagtgca
    10321 gcttttttgt tttttttatt aattgaagaa atgattattt ggcaaaacaa gcaagaaagc
    10381 gtatccttat aggaggaggg agtgaacagc caatgagaaa gaaaatgtta tttattccat
    10441 ttgctctatt gttaagcgct tgtagttcgg ttactcaaga atcagacaat acacatctat
    10501 caagcagtat atctgaaact gttaccagtc cagaaaaaag tagtaccaaa acggcttcca
    10561 ccactgaaca aacaatggct acctcaaata atgagaaaaa gactgcttta gaccagttga
    10621 aagaacaaca gccaaatgtt cccatgcctc tagatgtacc agtctcttca ggctatctca
    10681 atatcgcagc gacacataca aagcaaggtt attctatttt atattacaga acagatcgtc
    10741 ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc tattgcaacc tatctatatc
    10801 aatatgggtt tgcttctagt caagaaacaa tccaagtttt gcagccattt gagatcgaca
    10861 caaatggtca gcaagtggac ttaggttctc gaatcacagg gtatcagcaa ggtgcagcag
    10921 gttcaagttt tcttgaatgg caagaaggaa actggcgtat tcgaatccgt gggaataata
    10981 tagaaggcca agatccctta ctattagcta aagaaatcgt agcttatttg gaagaaaaca
    11041 gtctaccagc acctgaacaa tttggtaaaa ttactgttga tatgggtgat acgacgaatc
    11101 gagccgtaga agtaagctgg caagaaccga aaaatgccta tacgatcact catcaagatc
    11161 ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt gtgaaatcag cgcgctgatc
    11221 tgagcccatc atttaactcc aagtcacagg aagtaatcaa aaatagcacg ccgcattttt
    11281 gcataatttt ggcttaatac cttaaaagaa aactagaatt gcctcttcac acatgctaaa
    11341 ataaaacaga caaaaaagaa atggggatat aagttgactg aatacttgaa tgtaggaaaa
    11401 atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta tctcaacaac ggatttccca
    11461 gaagaacgtt acaaaaaagg aacggtgcta acactattcc aagacggaaa agcacctgtg
    11521 gaattaacag tcaaaagtca tcgcaaacat aaaaattttg acttattgag ttttgaagga
    11581 catccttcta tcaatgatgt ggaaaaatat cgggatggca tattaaaagt gtcaaaagac
    11641 aactctgtag atctagctga aaatgaattc tattaccatg agatcatcgg tctgcatgta
    11701 ttagaagatg gaactgagtt aggtaaagta aaagaaatct tgtcaccagg tgccaatgat
    11761 gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc ttccatatat cgcttctgtc
    11821 gtcaaagaag tcgatttaga agctggagtg gtccgtgtag aaattccaga aggactgatt
    11881 gacgatgaag attgatgtat tgactttatt tccaagaatg tttgaaggtc ctatggggga
    11941 atccatcatc ggtaaggctg tagataaagg attattagat atcaatatct ctaacttccg
    12001 cgattattca gataacaaac atcagacagt agatgattat ccatacggcg gtggagcagg
    12061 tatgctgctc aaagtccagc ccatctatga taacatccaa gccattgaaa aagctgcacc
    12121 ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt aaacgattcg atcagaaaat
    12181 ggcagaagaa ttttcacaag aagaacactt agtattcatt tgtggtcatt atgagggata
    12241 tgatgaacgt atccgcacat tagtgacaga tgaagtttcc ttaggagatt atgtccttac
    12301 tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca gttcggcttt tgccagatgt
    12361 gttagggaat cagacttcag ctcaaacgga ttcttattca accggtttat tggaacatcc
    12421 ccaatatact cgcccggcag aatataaagg gatgaaagta ccagaagtat tgacaaatgg
    12481 aaatcataaa ttgattgaag aatggcaatt aaaagaatca ctaagaagga cctatcaacg
    12541 tcggcctgat cttttggaaa cacttgaatg gacacctcaa atgacgaaat ttttagaaga
    12601 aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc aagtgacttt ttctaaaatc
    12661 ttctttacag taggttgata atatgttagt attttatggt gagtgcaaaa gcacttaact
    12721 attacgatat tccgctgtga gaaaagctca taagaatatt tggaataagg agaagaaaac
    12781 catgaatcca ttaatcgaag aattaacaaa agaacaatta cgttctgaca tcccagcgtt
    12841 ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt gaaggtactc gtgaacgtat
    12901 ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct ggaatcagcg aaacttatac
    12961 agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca ttcccattgc atacaccacg
    13021 tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt cgtgcaaaat tgtactacct
    13081 acgtgcatta cacggaaaag ctgctcgtat caaagaaatc cgtcgttaat catcgttatc
    13141 agactgagaa agaacccttg gtattcaagg gttctttttt tgttctctgt ttttttatat
    13201 taacgatttc tatttgggac atttttgatt aataaaaagg ggcaaaaaag gggcaacaag
    13261 ttacagtttt tctatatctt ctttcaattt ttcttttact ccctttgtca catgcaaata
    13321 aatcttttca gtgatatcac tattttcgtg cccaacgcga tcctgaatag catacagagg
    13381 agttcctagt tccgctaatt ttgaaatatg ggtatgtcta aaaatatgtg aactaagttt
    13441 tttatcaatc ttcatgtcag ctttatgatt tctcaaatag gtgttaattg ctgttagttg
    13501 gaaaggagtt cctttcgttg tttgaaatag aaattgacca ttcggattta attctagaag
    13561 ctcattgtaa atagctatcg ctttttttgg taaatcaatt tctcgcattc cagcagcagt
    13621 ttttgtagaa tcagattttt tcatatcagc tattgaacgt tctctataca tcatcgtccc
    13681 atttataact actgatgcat tattattggt aatgtgtaca tcgtctttac ttaatgcgat
    13741 tgcttctcca ggcctcattc cagtcaaata tagccactgg aaaaggagag aatatctttt
    13801 attgtgtgaa gttgtgaaat ttactagtct tttatattca tcatcttcta aaaacttatc
    13861 tttaattttt atcgtttttg actctcgttt ataatcaata actacttcgt tgataggatt
    13921 tttttctaca tagccttttt tcatagcgta tgaaaaaaga agattcaatt tggatttaat
    13981 tacactgacg tacttatttg acaagtcatc tttatatatc atatcttcaa aaatattatt
    14041 taaatctact gtattaatac cagaaacgat gtaagtctca ggtatctttt tctttatagt
    14101 gtttaagata ttattcgtag gataatatgt agactcctta acttgtcttt tgtaaataac
    14161 taaccattct tcgacaagct catgaaatgt tagatcaggc ttctgaagtg ttttttcgtt
    14221 taattttata tcaatttttt tatttaactc tagcaatgcc actttctgag tttctcgaga
    14281 tttattttta taagtaatgc tcactttttt tctttttcta gtttttggat caatatatcg
    14341 ctcattatat ttatatactt tttttccgtt tttatcagtt ttggtttcaa tccacattgt
    14401 tttcatctcc tatttgttgc tataatagga atggataagt aagccaatta tagcaggttt
    14461 atttttcatc acgtccacaa actttggcga gggagggggc gtgttttttt gtttagtagc
    14521 tacctttcca tgaagtatct gcgaagaaca cttcaaatgt tgttccatca tttttcactt
    14581 caaaatatgc tgttccttgt gcggatttac ccggttggat ggtttccgaa aagtaatctc
    14641 tactagctaa atcagattta atatctgatg aatcataaaa ttctaaataa cttgaacttg
    14701 catcaaaagg tgtagtccct gtgttttcta caagaaaatc tacctttgcg taaaataggc
    14761 cttctggttt atagaaatca tcaccgtaat cttttgatac agactggaca gttacgttaa
    14821 tagagttacc attttcagaa gtaaaaggaa ctgcttctcc tatactatgt gcaactgatg
    14881 ccgtctgggg ttctgaagac gaagttgtag ttgtagttgt agtagagctt gaatctttgg
    14941 attttccatc tttatctaag aaagttcgtt gattataata tacaacctgt tcagtttttg
    15001 gactaaccca aattaacatt gtttcatcat cagaatcagt tgaataagta tattggagca
    15061 tctctaattt ttttatatct tttgcactat ttccatgttc aattaattca gagtgctctt
    15121 tataataatc tccttgtgca tcctttgaaa tagatacaat taattcatcc cgtgtgatag
    15181 cattgtctaa ttctttgtaa acaaaatcat ttgtccattt acgtaacggt gaaccaagtg
    15241 tctgataaac ttgagttaaa tcatcaccaa cttttatttt atgaacgtct gatgaagtta
    15301 gtttttttgt ttcatattca gatgttgatg aaacagtatt ttctttagtg ctagcagaat
    15361 taggactagt ggagcatccg cttaatacca agcctaaaaa aagcaaccca aaaacaattt
    15421 ttttcatttt taattcctca cttctatgat atgatttttt tgtaggatct tagaaataag
    15481 gtttttagtc cgtgttgcag cacggacttt ttttacatta tataagaaag cgataagcgc
    15541 tttctggaag tccgtaaaga ttctttaatt cctcaattcg tttaggatat tgatcattgt
    15601 cttctttgta tagagaaaca atgagattag cagcaaagca attagcttcg ctttcagatt
    15661 tgcttctaga tgttcttgtt gatacatagt aactggataa gccacgatga aaaatagcgt
    15721 gacctaattc gtgagcgcaa atgtagaatc tttcctcaga gtctctcagt tcatcattta
    15781 agaagattat tgcacgacct ctaatttctt gaaactgtcc tttgggattc tcgataaaag
    15841 gaacgtattg aattttaatg cccatctttt cacaaatata aaaaggatta gcggactggt
    15901 atttccgctt caactcctcg actaaattaa tcgtatccat ctccataagc tcacatcttt
    15961 ttgccttttt ctttgtcttc tttcacaata tcccagaagg ttccaataag gatatctttt
    16021 acgcgttgga cctgttcagg cgtcagtgtt tctccgccat aagacatatt gacatttgaa
    16081 tctagtaatt tatcaagttc aactacttca tcttttgttg cccagtcagg aacttggttt
    16141 ctaccgagta aataatcagt agttacatta aaataatctg ctacaagttg tagtctttta
    16201 gtactaggag ttctattttt ccattgataa atcgtgtttt taggtatatt taattcttct
    16261 tccaattgag ttatacttat gcctctttta tgagctaatt cctttattct atctagtaaa
    16321 ttcattttcg cacctcacaa gctacgaaaa cactaataaa aaaattagtt aaaggtgttg
    16381 acaactaata aaagtgttag tatactgttt tcgtaagcta aaatattagc taaaaagact
    16441 tgataactaa taaacacttc acggtcggca aactctgaaa tgtaaattac taggcatttc
    16501 tgtgtcttat ttagctatgt ctatatacta ataaaaatat tagttattgt caacgaatat
    16561 tagctaattt tttagcttac agattatttt tttagaaagg agctattttt atgtctgaga
    16621 atttagactt aaaaattc
//
LOCUS       DAZKBH010000057        16497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000057 DAZKBH010000000
VERSION     DAZKBH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.057"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACG8OO_001730"
     CDS             complement(29..727)
                     /locus_tag="ACG8OO_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFP9045608.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACG8OO_001731"
     CDS             complement(720..2072)
                     /locus_tag="ACG8OO_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFP9045609.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACG8OO_001732"
     CDS             complement(2295..3695)
                     /locus_tag="ACG8OO_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9045610.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACG8OO_001733"
     CDS             3856..4593
                     /locus_tag="ACG8OO_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFP9045611.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACG8OO_001734"
     CDS             4718..5197
                     /locus_tag="ACG8OO_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFP9045612.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACG8OO_001735"
     CDS             5194..5961
                     /locus_tag="ACG8OO_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFP9045613.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACG8OO_001736"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACG8OO_001736"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9045614.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACG8OO_001737"
     CDS             complement(6864..7466)
                     /locus_tag="ACG8OO_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9045615.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACG8OO_001738"
     CDS             7572..7802
                     /locus_tag="ACG8OO_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045616.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACG8OO_001739"
     CDS             7793..8041
                     /locus_tag="ACG8OO_001739"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9045617.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACG8OO_001740"
     CDS             8147..8731
                     /locus_tag="ACG8OO_001740"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9045618.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACG8OO_001741"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACG8OO_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9045619.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACG8OO_001742"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACG8OO_001742"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9045620.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACG8OO_001743"
     CDS             10632..10811
                     /locus_tag="ACG8OO_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045621.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACG8OO_001744"
     CDS             10833..11858
                     /locus_tag="ACG8OO_001744"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9045622.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACG8OO_001745"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACG8OO_001745"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9045623.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACG8OO_001746"
     CDS             13430..14377
                     /locus_tag="ACG8OO_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFP9045624.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACG8OO_001747"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACG8OO_001747"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9045625.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACG8OO_001748"
     CDS             complement(15279..15521)
                     /locus_tag="ACG8OO_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045626.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACG8OO_001749"
     tRNA            15642..15714
                     /locus_tag="ACG8OO_001749"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="ACG8OO_001750"
     CDS             complement(15833..16471)
                     /locus_tag="ACG8OO_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFP9045627.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5622 a         2947 c         3316 g         4612 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttt
//
LOCUS       DAZKBH010000058        16444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000058 DAZKBH010000000
VERSION     DAZKBH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.058"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1404
                     /locus_tag="ACG8OO_001751"
     CDS             <1..1404
                     /locus_tag="ACG8OO_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9045628.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            1753..2385
                     /locus_tag="ACG8OO_001752"
     CDS             1753..2385
                     /locus_tag="ACG8OO_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFP9045629.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2973..4412
                     /locus_tag="ACG8OO_001753"
     CDS             2973..4412
                     /locus_tag="ACG8OO_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9045630.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4707..4925
                     /locus_tag="ACG8OO_001754"
                     /pseudo
     CDS             4707..4925
                     /locus_tag="ACG8OO_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5174..5389
                     /locus_tag="ACG8OO_001755"
     CDS             5174..5389
                     /locus_tag="ACG8OO_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045631.1"
                     /translation="MNAGIADMNLIKKNLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5606..5862)
                     /locus_tag="ACG8OO_001756"
                     /pseudo
     CDS             complement(5606..5862)
                     /locus_tag="ACG8OO_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6049..6960)
                     /locus_tag="ACG8OO_001757"
     CDS             complement(6049..6960)
                     /locus_tag="ACG8OO_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9045632.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6979..7983)
                     /locus_tag="ACG8OO_001758"
     CDS             complement(6979..7983)
                     /locus_tag="ACG8OO_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFP9045633.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7980..10103)
                     /locus_tag="ACG8OO_001759"
     CDS             complement(7980..10103)
                     /locus_tag="ACG8OO_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9045634.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10108..12555)
                     /locus_tag="ACG8OO_001760"
     CDS             complement(10108..12555)
                     /locus_tag="ACG8OO_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9045635.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12542..12931)
                     /locus_tag="ACG8OO_001761"
     CDS             complement(12542..12931)
                     /locus_tag="ACG8OO_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9045636.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12991..13494)
                     /locus_tag="ACG8OO_001762"
     CDS             complement(12991..13494)
                     /locus_tag="ACG8OO_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9045637.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13507..13731)
                     /locus_tag="ACG8OO_001763"
     CDS             complement(13507..13731)
                     /locus_tag="ACG8OO_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045638.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13835..15745)
                     /gene="ltrA"
                     /locus_tag="ACG8OO_001764"
     CDS             complement(13835..15745)
                     /gene="ltrA"
                     /locus_tag="ACG8OO_001764"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9045639.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4897 a         3259 c         2931 g         5357 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcgt caagtgaaag tagtacgatt
      301 tcaagtaccg aatcttcaac aacttcaagt agtacaagtg aaataacttc ttcgaaagtg
      361 acaaaaccaa accatataga atcttcaaaa aaggaagcgc caaaaacgga aggacaaaca
      421 aaaacttttt cgacaccaca gcaaatagaa tcaacagaat ctttccaatc acaaccaata
      481 ctatctggta atgaagaaat tcattttgac aaaaatgaat ctgtagaaaa ttttattatg
      541 aaaattggtg agtctgcgag aaaaattggt cagaaatatg atttatatgc ttctgttatg
      601 attgcacaag cgatcttaga atctgccagt ggtcaaagcc agttagctca ggcaccgaat
      661 tacaatttat ttgggataaa aggaacttat aatggaaact ttgtcattat ggtaacaaac
      721 gaagatttgg gtaatggaac gctttatacg acccaatcaa aatttagagt gtatgaaaac
      781 tatgaggaaa gctttgaaga ttatgccaaa ttattaacta agggaattag tggaaataaa
      841 gatttttatg caggagcatt aaaagctaat agtaaaacat atcgagaagc aactaaattc
      901 cttacaggac ggtatgcaac agatacacaa tactatttga agctaaatga attaattaaa
      961 acctacgatt taaccaacta tgataaagaa gttaaaggtc cacaaatgag taagactgga
     1021 taccaagtgc ctattcataa ttattcaata accagtttgt ttggaaatcg tggaaatgaa
     1081 tttcaccgag gacttgatat ggcagctaca cagggtgagc cgattcatgc agttaaagct
     1141 ggtaaggttg ttaaagcaga gtttcactac tcttggggaa attacgtggt gatacaacac
     1201 gaagatggat caaccgcgtt atatgctcac cagcaacaat acctagtaaa aactggagat
     1261 aatgttttgc aaggacaaat aattggctat gtaggaagta ctgggaatag tactggccca
     1321 caccttcatt tggaaatttg caaagatagt agtctttctc agagcatgtt agttgatccg
     1381 aaaaccttat tattcggaga ctaaaaaagg taatcaatct gacaaagtaa caagaaaatg
     1441 gttggattct tagacctttg atcaggatat tgagactgga attgatatct cgattacgat
     1501 gggtttgaca aataaaacaa gtccattatc gaatatcgag tgtattattg ccgtctttat
     1561 gctcatattt tgagcaggtt tcactagatg aaaacaattt atctatcttg aggatttcgt
     1621 gtacgtatcg ttcagtcttg tattgtaaat tagtcataaa atcagactaa gaaaaattgg
     1681 aaatattttt ttctgattta ttaaaagtta attgaaatta tatggtactt ctttcttggg
     1741 agggtaggtg cagtgaagga aagatatggt caccttactg ttgttgagga gattactcat
     1801 aatggatata aaaaatatcg ttgcctatgt gactgtggga atgaaacgat tgtatatcga
     1861 tcaaacttac tatcaggtag aaccaaaagt tgtggatgtg gaaattataa aaataggcat
     1921 aaatataaaa atttgacaaa taaaactttt ggtaagttag ttgcaatcaa gccaactaaa
     1981 ctgcgaagtg aaggaactgt tgtttgggaa tgtcaatgtc tgtgtgggag aaaaaagtta
     2041 gtatcggcaa agcgacttct aaaaggtgat gtaaaaagct gtggatgttt gaaatcaaaa
     2101 aagcatcaca ttgaaaataa aaaattcgga ttacttactg caaagtatcc tttggacaat
     2161 aactgttcat ggaaaacaaa atgggtttgt cattgtgagt gtggcagtat gtgtgagata
     2221 agttatagta acttattttt tggacatact caaagttgtg gatgtttaaa aaggcaagaa
     2281 acaaggacat gtgttgaagg gacagtaata gagtgtctaa cgtcaaaatt accgaaaaat
     2341 aatacaagtg gtgttaaggg agtttatcgt tctaaagaga aatagattgc gtatataact
     2401 ttaaaaaaaa agaggtacta tttaggatcg tatgattcta ttaagagtgc agctcaagct
     2461 agaaaagatg ctgaaaataa attgtttcaa cctttgattg acaaatatcg aggtaatcat
     2521 taattattgt ggataataat taacagtgat atataaacat ctataatata ttattcatac
     2581 tattgatatt tagtataatc ctatggaatt gaatatcata gcaatgtatt tgaaaagcaa
     2641 attggaaaac aaataggtgc aacaccataa atatgaaaaa attgggaaaa agtagaatgg
     2701 aaatttgctt acgtctcacg agatagctca cactattaat ctgaagaaca atgaagtcga
     2761 cttttagaaa ttgctagaag aaagtaaagc gaattatcac tagttactat agagtaagtt
     2821 caaataaagc atctccaact tacatggtta catgaggata tcaaatcgaa attactcaag
     2881 cttcctatgg ctagagtcgc gagaagttgt tgggcagtag acataggtta tattgttctt
     2941 agctacacga tcattagaaa ggaaatccta ttatgtatag ttatcaagaa gatgcaaaag
     3001 tccaaaaaaa tcgctggtgg attttgattg ctatatctat gtttaccttc atgtccacat
     3061 tggatggcag tattgtcaat attgcactac ctaccatctc agatgatatg ggcataccaa
     3121 tgaatcaagc ggaatgggta gtgtccgttt atctgatgat tgtctgttct tgtttgttgc
     3181 tgtttggtaa aattggcgat agccgtggga aaatcaaagt atttcggatt ggtacgctga
     3241 tctttgtctt gggatcgctg ttgtgtgggt tcaatcattc gctaggtatt ttattgttcg
     3301 cacgggtggt acaagccatt ggtgctagta tgactatggc tacaaatgcc ggaatcatca
     3361 cggaagtctt tcccatacat gaacgtggtc gggcattggg agcaataggt gcgtttgttt
     3421 cattaggctc gattgctgga ccaggtcttg gcggcttgat tttgtcccaa ttttcttggg
     3481 cgtacatttt ttggatcaat gtaccggtgg gaatcgtaac gatgttgatt gctgaaaaat
     3541 tcttaccaaa agatatcacc atgagcggcc atacaatcga tggagggggc tttgctgcca
     3601 ttgcggctac gattatcagc ttttttggag caatcttttt ggggcaagaa agtggctttt
     3661 tccatccagt cccccttgta ctgttcgtaa tttcagtgat tgcctttgtc agctttttaa
     3721 aagttgaaaa gaaagcagcc tctccattga tttctttcgg tatttttaag aacaagattt
     3781 ttaccatgag cttgattaca gccttgttga ttttttcttc caacttcttt gtcaatgtag
     3841 tgattccttt ttatctgcaa aatgctcgcg ggttgagtcc tagctatgct gggctgttga
     3901 tgatggtctt tcctttgatg atggtcgtcg gggcgccttt aagcggctac ctgaccgacc
     3961 gggttggccc ggagattttg gtattagtag gcttgggctt attgtcggta acccaggtca
     4021 tgtatatgtt tttgtcagtg gactctcctt tgtggctata cattgtcgct actgccatca
     4081 tgggtgccgg caatgccttg ttccagtccc ccaataatac gatggttatg agctcggtca
     4141 ctatcgaaaa cttgggactg gcaggctcga tgaattcctt tgcccgaaat ttggggatgg
     4201 tcagcgggat ttcgctttct acgacggtct tatatcaagc gatgtctgct aagatgggcc
     4261 agcaagtaac taactatatc gcaaatcggc cggatgtctt tttatatgga atgaagatta
     4321 cctttttagg cagctttgtt ttgtgttttg ttgccttttt cctgactatc tggcgtatca
     4381 aaaaacaagg aggtattcag aaaaatgtct gaaaaaacca ctgttgaaga atttttggag
     4441 gcctgcgtat gggtgagtgg gatttccatg gatgatggaa gatgaagtga acttctaaaa
     4501 gccgaaaaac tcaagtatta tcttcaaaat actagtgctc aatgagatgc ttgatttgat
     4561 cacaagaaga ctgcgaaagc cgctggcaac cgcaagctag gtatcccacc gatggactac
     4621 gttttagagg ctactggtta tgaatacgga gccaacgctc tcatctgcat aagaatacat
     4681 cacccagatt atgtaaagat tttcgatact tctgtcaaaa aatcattaag atagataaat
     4741 gatttccgtc tagtcaaatt ggttcagaat gtagacacac agatggtaag aagtcactca
     4801 caattcgaga atggacttgt cctatttgcc gtatacatca taatcgaaac gtcaacgata
     4861 gtatcaatat tttgaccaag ggctatgaat acaagcattg ccttagaaaa catcaagaac
     4921 cttaggaaca acggggatag cttggttaat aagagatacc gctgtttagg cttgcaagta
     4981 tactctttta tcaagaattc cgtggcttta gtcatgagag gcttaatact atgaaaattt
     5041 tctttagcat ttttgattta ggttcctttt ctaattataa taagatgacc actacagtaa
     5101 cctactgaaa tatataaaca gccactgtag taccattttc aaagtagtct atgagaggag
     5161 aatcttcatt cttatgaacg ctggaattgc ggatatgaat ttaataaaaa aaaaccttaa
     5221 tgattttact tccaattcaa tatcaaaagg tactggtatc aatttaagca caataaaaaa
     5281 gctgaaaagt ggtgaaagat cagtggaaaa gctgaatctt ttagatgcta taaaaataac
     5341 ggaatttgct atgaaaaatg gaaaagctga gattgaaata tggagataat tatcgtctag
     5401 tgaattactt gccacttagc tgaatttaac gatccttggt gcttgaaggg ggagattctt
     5461 gggaatagag catacttgca agcctatgtc tttactaaca acgatatctc ttattaacca
     5521 agctatcccc gtagttctaa ggcttttact tttatctaag caataatcgt attcaaagga
     5581 aatttttgac ccttagaaag tctcatttta tcagacgtga gcttaggcat tgctgaattt
     5641 tgttaattcg gttttaggtt gtgctttgag cataatatga aagtattctt tatcacgatt
     5701 tcattcatct aaggtaatgt gatgtgattc tgaaattctt tcaaaggcag tttttgcata
     5761 agctgacatt acatcatcaa tgacatgttt acaatgtttc actactaaaa taagggggta
     5821 accaagaaga aaaactgaat gattatttgt atctaattcc atggtatatc aaccatttcc
     5881 tatatagact gattatgaca tggattaaaa taaaaaggca ctatgtctta attgtcggtt
     5941 ttcgagttac cgaatggcat tcatcaccca cctatagagg atgggcgact tctgcctgaa
     6001 ataagttaca ctacttataa cttctaataa aattagattg agtcattatt atttaacatc
     6061 aacgattttc cagtttccat ttttttgtaa tactaatttg tactgaaagt agctagtggt
     6121 ttttgataca ttatcaaggt attttatctt tgtactcact agaaaatttt ttccgttttg
     6181 tagataaact ggattctcta actccgaaaa ctttaagtca atattgattg gtcgcatcgc
     6241 accattttca acataatact ccaattcttt ttcagaagct gatggataaa gtttaaaaaa
     6301 tgtttctaaa aaatcggtga tttcttccgt tgttttggca tcaatttctg aatcgttttc
     6361 tacttgtttt gcttcatatt ttgccttgtc tggctttcca atcattgttg gcaaactagt
     6421 tactaaatga ttcctgtttt catcttcaaa gattgtcact cgatatgcag aagaaattga
     6481 ttgcgtcttt tttccctctg taatatgttg ctccactaaa aatgaaacga caaattcttc
     6541 cgagtgtttt tcaacatcta gaatttttac agattgaact tcggaactcg tgggaatatc
     6601 tgctcgaacc atatcttgga ttaaagcgag accgttttct gtaaggtact gttcgagatt
     6661 actcattctc ttttcaattg cttctttgtt atttttccat gaaaaatatt ctttcgcaaa
     6721 atcctctgta aaactttcaa cgccagaagt attcactacc ttctctttta taaccacttt
     6781 ttcatgagtt gtatgttgat caatggcggt gaaattttta taaacgccaa aagcaagact
     6841 acaagacaat aaaagccaaa gggcaagcac catttttcga tgcttaccca cggatacaac
     6901 tcgttctttt ttcttttttg ggctcttttc cttttgattc cgttcaattt ttatcttcat
     6961 tctttttcct cttttctctt atttgtatcg gccagctccg ataagatgct gccgccaata
     7021 gttttccgtt agattcgtcc accccactgg atctcctgca tgaaacatgc gattctcccc
     7081 tgcgtaaatg cccacatggg tcacataagt accggcatca taggttgaat gaaagaaaac
     7141 taaatcacct ggtttggcat cttttagatc aatatgagtc atggcatcat attgatcctg
     7201 agccacccga ggaagtttca ggtcaacttt ggcataacac cattgggtca agccactaca
     7261 atcaaacccc gtttctggac ttgagccacc aaacacatag gccgttcctt catacttaaa
     7321 tgcctcatca aaaatttctt ggactatttt gtcatcaaac tgagttgtag ttaggtattg
     7381 cttcactaat aaacagtaaa acatattgcc ataattatac cgccacccac cattttcctt
     7441 aatcgcgatt gggttggggt aatcgacttt ttgaccacct gatttttcct ttgaaaaaga
     7501 ttcagctaaa gcaaaactat gcttcttccc gtttttcccg acatgatcta gatacccgcc
     7561 accatagtta taggcctgaa taatactatc ttgatcacac cctagttctt tggaacgctt
     7621 aaccaaagac gcaaaataga gacagccttg tttgatggat gcctcggtac ttaatgaatt
     7681 aggtggtaat ccggctgact cagaactttg cataacgtct tccattgtgc caccagattc
     7741 cacttgaata atcgccaaaa gaattggaat atactctgtt atatccgatt ctttggcata
     7801 cttttccacc attgcacggt gtttcaatac ttcttctgag acactagttc cgccaggaga
     7861 agtgccagaa ctaccatttc cctcactgtc ttcaccaaac aacaaaccca cacacatcaa
     7921 aatacccata aaagagatgg tcagaaaagt taatgaaata acacccactt tcttcaagtt
     7981 cattttttct cacctgacct tgattttgga cgatggttca ttcttttttg tactttttga
     8041 ttggtctgct ttggcagagt ggacctctgt ttcacttgtt ggaaagaatt tttcttactt
     8101 gctgattggc ttttcccttt tgaaatgatt tcttgactag ttagttttcg tttgatttcg
     8161 ggattcagat ttgctctcac aatccgcttt ggtgcttctt ttttcagcat catatctttc
     8221 tgaggttgag ctgctttatc tattggaata ttcgcccttc tttttccctg acgatttgtt
     8281 gcttcactca gtatcttgcg tttttcgtcc attttcgcat ttcttcgttg tcgttgtttt
     8341 tctcgcaaat ccactcgatc cgctttttct tggaacagtc cgcgcttgaa ttcttctggt
     8401 gcttttttcg acttttcgat tcctttatgg agattgtatt tcaaattcgt tggcgtattc
     8461 cttacctgat cctttacttg tttagtctta tctttcacca aagcttgggt atccaaaact
     8521 ttgccgattt ttctcccagc caaattcatt cgggattgtt tcttattatt tgaaactgga
     8581 ttctccttag ttaagtcaga tagtatttcg tgatcgttcg gtaatcgtga attctttcgt
     8641 agagaagaac cggatggctt aaatttggat tttgattgtt cctttgccaa cttatttccg
     8701 actgctgcac caccaccaaa agccaaagct cgtcccacat ttcgattgag ttgccgcaat
     8761 tttcgattca ttaacatttg cggttttcgc atgacgcgtc ttcccattga ctggctatca
     8821 ttactctgga ggttgaacat actcataatg tcgcccaatt tgaaatagat accggcaaag
     8881 gtcacaattt gcagaaaagc aaccatgaaa aatggatagg ttgccgaaat attgaaaaac
     8941 atcgtggaga tgctaaatgc agtcgtaatc accaaagtaa cacccgctcg catcataatc
     9001 gtattgaata aacgaataat cgccttcttt cctaaacttt cataagtcgg caacatggac
     9061 aataagaaac taattggcag aaacatcgca aagataatga agagaatttg cgagaacaac
     9121 ataatccctg ttagaagaaa aacaaagaag gaaatgccaa tattgaacaa taccaaaaag
     9181 accacggtcc ctaaacggtt catggtcttg gtaatactga gattcgcatt gtcattgtct
     9241 tcaatctctg ccttgaccgc ttcttcccga tctttgcctt tgttttcatc tggactcaca
     9301 gatagaatct tgttgacccg gtcttcacca atttcttcta tgttggaatc atcaaattgc
     9361 agtagtaacc aaggttgttg gacttgaatc gaaaacaaac tatcccgaat caaatccaca
     9421 ctatcttttc cttggctttc tgaattgggt acaacgatct cggttccaag ggtaagggcc
     9481 gcttcactaa catctgagct aaagtcattg atttttgtga tataggtagg cgcataagca
     9541 atgaacgaac ccgatagtag aaaaatcatc acaaaattaa tcacggctcg aactgctttc
     9601 gtggtttctc gttttaataa tccggtatac gccacataca cccccagagc taaaatcatc
     9661 aataatagaa acccaaaata aaatccggag gtctggagtc cattttcagt aataccagcc
     9721 aatgtttgga tattctttcc aatactttca gccgtatccg aaatgaaatc taatttgtaa
     9781 gcctcttgaa ccacataacc cgtggcgttc gacaaataca aactcaccgt ccaaatgaaa
     9841 ttagtaatgg cataaagacc atacatcacg cttttcccta agccatcacc ccaattccaa
     9901 ggcaaccaat cccaagagct atccacaaag aaatctagtt gatagttgtt cagtgaatat
     9961 ttggaatata gatttcctgc ttcaactgta tcatcaacta gccctgtcgc ctcggctacc
    10021 gtaccaacta aactcaatag aactatcaga gtgagtatgc taataaaaac gattcccaca
    10081 atccgaaaaa gtttcttttt catcccctca ctccattctc tcttgaacag gtggacgggt
    10141 atcaaaggca tggaagagtt cttcaaagac tggatgtacc tgaaccacgc ctacccgacc
    10201 atacaaatcc tgcatcaagc attggccatt ttccaaatct cgtaggcgtt tttgattgct
    10261 ttcatcttct cgatccaagc caaagaattc caaggttttc ttgatttcat gaatatcggt
    10321 actgcgaaac gcaaacttca acccgatatt attcttcaag cgttcatcca gtaaatcatc
    10381 actattttgc gtgacaaagt aaaccccggc attcatagcc cgcccagcac gaacgagctt
    10441 attcgataat gttttccctt gcgctacttg aagaaaactc cacgcctcat ctaagtctac
    10501 aattttgaag atttctcgat tactatggat aaagtccaag gcaaaagtgg aaatgacgat
    10561 tagcatggaa acactaagca attccatggt ggtatattct tcaaaagtgg tttctgcatc
    10621 tggtaaaacc aaatccgcta cttgaatgat atttaactgc ctatctaaac taatcgactg
    10681 ggtaatcgaa ccatctgaga aaagcaaatg ggcaaaatca taatcggtaa aggactcgat
    10741 gtgatccgcg atattttccg caactggtga gccttctttt cgaagctcat taattacttg
    10801 taataatccc cgttgattgc tttgggtaac cgtccgaatg gccttacgca acactggaaa
    10861 cttctcccca tctcgactag aaattcctgt taaaaaggtc agaatatcaa ttgctagact
    10921 ttcagaatcc ttctttttct ccataatcac ataaggatca agtaatcctt gattttttgg
    10981 cttactagtt aagttgagaa tattgatttc atccgcaatt tctggcaagg tttctttcca
    11041 gccaccccgt tccccttttg gatcgacaat cacagcttgt ccaccaaaaa ggacggcgta
    11101 ataaactaag agattgttac taaaggactt tccgccaccg agtgaaccga ggaatgccgc
    11161 tgctagcgca ttggtaactg aatccttgac cccttgtgag gccaatgccg gtttcaggta
    11221 aacattgcgc cccgtatcta cgttataacc aaagtaaatg ccttccaact cccctaacat
    11281 ctgagtggcg ccaaaaccaa gtccggcgag aaaatcagac gtaacatact ggatataatc
    11341 attcatgtat cgcttactag aaggtaagaa ttcttcatgt agccccatca tatcgccaaa
    11401 aggccggact agttttacat tggtatcatc ataaaaatct agcacttcat cacaccggcg
    11461 ctttagttca tctactgatt cggcacttac tcgaaccaca tagctcagtt tgtacatgga
    11521 ttctttggac tgatccaagg tagtttccaa ttcatctacg gaatccaatg catctaagac
    11581 attggaattt gtttcattgt cagattgata ggcatggttg tccaagtctt ttaattcctt
    11641 tttcttattg cgaacggtag caagtgcctt tttgttcgtc acaatctcca cattcatact
    11701 agtgtcaatt gggaatgtaa attgttgttg ctggtaatag aaaagttctg aggagggaaa
    11761 ctccatttcc cccacaattg tattaattgt taagtacgct acatatgatt catgattctc
    11821 atgttccatg cataagtacc ggggcttttc ttcaatcaaa caacgactgg gacgtaataa
    11881 gtcgtaacgt ttcaccaaag tttccgactt cagctttttc tttggtagct gaaattcata
    11941 ttcttcaaat ggtgtaccct tttcgccata gatatgttca atcaaataca tcagatcact
    12001 cggtgtgact cgtctgactt taaatcttcg agccaattta ctttccatta gtttttcaag
    12061 cttggtatat cggcgaattt cttcattgga taaggaaaca aaatcgccca tcaaatgatg
    12121 attcactcca tagacaaatt cttgaaagcc ggagaagaaa gatttcttga gattctttag
    12181 attgacctct tcatctgtcg caatcagttt gaagccaata aaaaagcgat aatcaatctg
    12241 actatcgcca atcatggata ctaatgcctc tgtctgtaaa tcaattcgtt gtttcgctac
    12301 ttctgttaat cgcccggtaa tttccttttt tgatcgttct tgcgtggcac gaatactact
    12361 ttctgtggca atttgtaagg catgaatctt gccttcacga ttttgcgcaa tcaattgacg
    12421 aaagttatcg tggacttgat atttctgttc aggtgacaaa aaggagtaat tgtaaggtat
    12481 taactcatag taggcaaaac attctccctc ttgattaaag acaagattat tttcaatata
    12541 cttaatcggg tacatagttc acactcctta ccagagtaat gggttcgtcc catcgtttct
    12601 tctgttcttt gactttcttt cctgcgaaag tgaccttaaa tcgtaagtga tagctaataa
    12661 aagatcgtaa aaatcccaat ggtttcttac cgtcaaacgt tttttgcgac atgaaccaag
    12721 taactgccac cggtatacct aagtatttta aaaaggcccc ctctatcata gatagtggag
    12781 gcacatgggc aaatacgatg acaaacagca aggataaaac aaaccaagcc atctggttaa
    12841 aagtcacagg aaaaggcagt tgaaagtcat taattgcata aatgaccttt tcaactgccc
    12901 aaatactggt gtagcttttt attttcttca tcaaatcatt cctttaaaaa gaaggatagt
    12961 ttagaaatca actacccttc aataattttg ttaatatgga tactcaaaaa cacctcgact
    13021 cgttaccaaa taattgcctc caatctccaa gtctctacca cacgcatcaa aatcaatata
    13081 cgtcttcaag tgttctggta cttctccaaa atcaccagct tctacaatca agaaatatgc
    13141 tacatcactc atatcatcgc aatctggata atagataata tcatccacat gctctaccat
    13201 ctcctcaaaa gaattgaaga aagcatgttg aatctcagaa gcaacttcac caactggcgt
    13261 tccttcaagt tcctccgcta agccacacaa ccgattaatt tcctcaattg gcgtatactc
    13321 atcaatctca aagggaagtt cataatcatg aattgcatat tcctcgtatt cgtcatttaa
    13381 cccaattcgt tctttcactt catcaaaatc gacaggtggg gtaaaccacg cccccaccaa
    13441 ttcaccttcg ttatatttcc cgaggttcgc aatataaaca cgcatctgtt ccatcgtgct
    13501 tcccccttac gcaccgacaa ttcggttaaa caaactgagt aagacatcct tcaccccaga
    13561 agcattaaag accaatccga ccgcaatcaa agcaatcacc aaaaagccaa tcagtttgga
    13621 aaactcccgc ttaaatccta aaaacaaacc aatgaccaca attgccagta agactaagga
    13681 ttgggcattg gttaaaaacc attgatatat ggataggtaa gttcctgata ttataaatat
    13741 ctcaaacttt tccccccatt cacacggagc gtgcgacttt caccgcactc cgcgttccat
    13801 cgtgtttata ttgaaaagta tttatgtagt agacctatat caagtttaat tcttgattgc
    13861 cagctaatac tctatattta tttagtctta tgagctggac tttatttagc ttcaatagag
    13921 ctatatattt ttgtattgtt tcttctgttt tagcgtgtat tagacggtgt accgcctctt
    13981 ttaccaaaat aagattttta taatcgtcat ttccaccagc tccagttggt acaatatgat
    14041 gacaatgtat ttcattagct tgttgaaatt cttccagagt tatagcacat tttccatgtt
    14101 gagcagaaaa tagcgaaatt cgattgtctg cgtattctat tgtgcgattt tccaaagata
    14161 atttcattaa ctggtacatc agtaatttat caacacttag attggtgtgg ataaattttc
    14221 ttccctctag agtgtagcaa ttcgctaaat acacctttgc cattggtttt ctgtgttgga
    14281 tatatccaac agggaaaata ggttcgccct cgccagcaac ataccgaagc attttagatt
    14341 taccatatct tgttgcctcc tttgtcgtaa gttgtctgcc tgtttttctc aatctgcccc
    14401 ttttgtttgt acctaatctg ttagtgaaga tagccattac agctctattc agtttcatac
    14461 aatccagatt tacattcgtt gcaatctgat aataattttg catacctaat accattgaat
    14521 tataaagtct tgtttcttct aattccgttt tcccttttga gggagaagca attttctttg
    14581 cctgtgatac cagcttgtct ttttggtttt tgaattgttt atcacttata tgtgatttaa
    14641 caactaactt ctttcttctc ggtatcattt taatcttgaa accaagaaag tcggagtatc
    14701 tttttctaac atttacaatt cttgtttttt cttgtgatac ttccaatttt aatcgttgtt
    14761 caatccattg agtgattgct attttggctt tttcagcact ttccttgtag cggcagaaaa
    14821 ctctaaaatc atctgcataa cgaaccatat acatttcctt taactttgtt ttcttcattt
    14881 ctttgtaagc gttgctttta catgggctac cgcttttatt gatatgaacc acatagtttt
    14941 ttgtaacagg attttcctgc cattggcttt ctacccaatg gtctaactca ttaagaacaa
    15001 tgtttgctag tagtggggat attattcccc cttgcggagt tcctttctct ggatatgtta
    15061 ttgtaccgtt agtcattttg attggagctt ttaatatcct ttttaaaatg aatatcaagt
    15121 gcttgtcccg tattcccata gcccatatct gttttatcag cttggaatgg ttcacgttgt
    15181 caaagaaacc cttaatatca aattcaatca cataatgtag ctggctgatt tgtaggcggt
    15241 tataggttgc ttttattgca ttttcaactg acctattagg tctaaaacca tagctgtttt
    15301 cactaaattt agcttcgcag attggttcta atacttgttt tatacattgt tgtataagcc
    15361 tgtcccacat acaaggaata cctagaggtc tggttttacc atttggtttt ggtatttctt
    15421 ttcgcctcac tggcttaggt cgatacccat gtggactgcc tgcaacaatg tatcttactt
    15481 ttttgacaac tgtttctgct ggcaatttgc caatatcttt aattattgta ccgtctgttc
    15541 ctgctgtttt actgcctgca tttgctttga tatttcgata tgccagtaat atattttctc
    15601 tcgataggat aatatccata agatgagtga aaatatctcc attcttactt ttggcatata
    15661 aatcatcaaa ggtgttttgc atttcgtaat attcagcgtg tcttaaatct tctacacaca
    15721 atgtttcatt tttattttta ggcataaggc ttcactcctt tacttgaaat tgagtgagct
    15781 tttttatctt actcgtaatc cttattgaaa caacataata cttttctaat atatacacga
    15841 cttgagggca ttcctccatt ctcattacaa gaatttctta ggttcgccct ctgcttttca
    15901 gcattagttc aactgcttat tgttcttcgt cagtttattc tcttgatgag aacccaatac
    15961 ctttcctcgt tccgataatc ttatctatac atatatatcc ttaggtcact agctataagc
    16021 ctgctggctt gataccgcct gtaacgttat atggcatttc ataacctata ttcttacttt
    16081 gccacaccag ctacgatttc tcgtaccttt gcatttctac aaagttcaaa tttagacccg
    16141 tacattcgct gtttcgtcag ttccttatag aacattctaa ccatagatat tttatagacc
    16201 cccgacctgt tggatacgct acccacctct ggatagtttt cgatagaaga tatatgttca
    16261 tcaactatta cggtatcttt cagccgtctt taccgagctt caaacattct gagtttcctc
    16321 atacaatgct tgtcggaggc tcagggcagg cgtttcagag cgttacttca tcattccacc
    16381 tgtcagatta tcagttctcc taataaccaa actttttcgt tcttttatta gtgccttagc
    16441 tttt
//
LOCUS       DAZKBH010000059        16342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000059 DAZKBH010000000
VERSION     DAZKBH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.059"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..258)
                     /locus_tag="ACG8OO_001765"
     CDS             complement(<1..258)
                     /locus_tag="ACG8OO_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045640.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ACG8OO_001766"
     CDS             complement(274..924)
                     /locus_tag="ACG8OO_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045641.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ACG8OO_001767"
     CDS             complement(935..2107)
                     /locus_tag="ACG8OO_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFP9045642.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ACG8OO_001768"
     CDS             complement(2126..2998)
                     /locus_tag="ACG8OO_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9045643.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ACG8OO_001769"
     CDS             complement(3020..3940)
                     /locus_tag="ACG8OO_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045644.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ACG8OO_001770"
     CDS             complement(3940..4209)
                     /locus_tag="ACG8OO_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045645.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ACG8OO_001771"
     CDS             complement(4206..4586)
                     /locus_tag="ACG8OO_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9045646.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="ACG8OO_001772"
     CDS             complement(5053..6516)
                     /locus_tag="ACG8OO_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9045647.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="ACG8OO_001773"
     CDS             complement(6580..9597)
                     /locus_tag="ACG8OO_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9045648.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="ACG8OO_001774"
     CDS             complement(9748..10587)
                     /locus_tag="ACG8OO_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFP9045649.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="ACG8OO_001775"
     CDS             10732..10977
                     /locus_tag="ACG8OO_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045650.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="ACG8OO_001776"
     CDS             11040..11462
                     /locus_tag="ACG8OO_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045651.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="ACG8OO_001777"
     CDS             complement(11556..11873)
                     /locus_tag="ACG8OO_001777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045652.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="ACG8OO_001778"
     CDS             complement(11900..12106)
                     /locus_tag="ACG8OO_001778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045653.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="ACG8OO_001779"
     CDS             complement(12087..12269)
                     /locus_tag="ACG8OO_001779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045654.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="ACG8OO_001780"
     CDS             complement(12283..12633)
                     /locus_tag="ACG8OO_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045655.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="ACG8OO_001781"
     CDS             complement(12659..12961)
                     /locus_tag="ACG8OO_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045656.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="ACG8OO_001782"
     CDS             complement(12964..13197)
                     /locus_tag="ACG8OO_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045657.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14101..14631)
                     /locus_tag="ACG8OO_001783"
     CDS             complement(14101..14631)
                     /locus_tag="ACG8OO_001783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045658.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(14628..15743)
                     /locus_tag="ACG8OO_001784"
     CDS             complement(14628..15743)
                     /locus_tag="ACG8OO_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9045659.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="ACG8OO_001785"
     CDS             complement(15749..16147)
                     /locus_tag="ACG8OO_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9045660.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4329 a         3420 c         2562 g         6031 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aa
//
LOCUS       DAZKBH010000060        15874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000060 DAZKBH010000000
VERSION     DAZKBH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.060"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(310..732)
                     /locus_tag="ACG8OO_001786"
     CDS             complement(310..732)
                     /locus_tag="ACG8OO_001786"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9045661.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(746..2152)
                     /gene="atpD"
                     /locus_tag="ACG8OO_001787"
     CDS             complement(746..2152)
                     /gene="atpD"
                     /locus_tag="ACG8OO_001787"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9045662.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2175..3077)
                     /locus_tag="ACG8OO_001788"
     CDS             complement(2175..3077)
                     /locus_tag="ACG8OO_001788"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9045663.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3091..4647)
                     /gene="atpA"
                     /locus_tag="ACG8OO_001789"
     CDS             complement(3091..4647)
                     /gene="atpA"
                     /locus_tag="ACG8OO_001789"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9045664.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4670..5212)
                     /locus_tag="ACG8OO_001790"
     CDS             complement(4670..5212)
                     /locus_tag="ACG8OO_001790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9045665.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5199..5723)
                     /gene="atpF"
                     /locus_tag="ACG8OO_001791"
     CDS             complement(5199..5723)
                     /gene="atpF"
                     /locus_tag="ACG8OO_001791"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9045666.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5814..6029)
                     /gene="atpE"
                     /locus_tag="ACG8OO_001792"
     CDS             complement(5814..6029)
                     /gene="atpE"
                     /locus_tag="ACG8OO_001792"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFP9045667.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6082..6801)
                     /gene="atpB"
                     /locus_tag="ACG8OO_001793"
     CDS             complement(6082..6801)
                     /gene="atpB"
                     /locus_tag="ACG8OO_001793"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9045668.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7103..7567)
                     /gene="smpB"
                     /locus_tag="ACG8OO_001794"
     CDS             complement(7103..7567)
                     /gene="smpB"
                     /locus_tag="ACG8OO_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9045669.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(7589..9946)
                     /gene="rnr"
                     /locus_tag="ACG8OO_001795"
     CDS             complement(7589..9946)
                     /gene="rnr"
                     /locus_tag="ACG8OO_001795"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9045670.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(9949..10704)
                     /locus_tag="ACG8OO_001796"
     CDS             complement(9949..10704)
                     /locus_tag="ACG8OO_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9045671.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10807..11043)
                     /gene="secG"
                     /locus_tag="ACG8OO_001797"
     CDS             complement(10807..11043)
                     /gene="secG"
                     /locus_tag="ACG8OO_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9045672.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11161..11439)
                     /locus_tag="ACG8OO_001798"
     CDS             complement(11161..11439)
                     /locus_tag="ACG8OO_001798"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9045673.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11552..12520)
                     /locus_tag="ACG8OO_001799"
     CDS             complement(11552..12520)
                     /locus_tag="ACG8OO_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9045674.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12735..12779
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12803..13777
                     /locus_tag="ACG8OO_001800"
     CDS             12803..13777
                     /locus_tag="ACG8OO_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFP9045675.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13782..14357
                     /locus_tag="ACG8OO_001801"
     CDS             13782..14357
                     /locus_tag="ACG8OO_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFP9045676.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14424..15563)
                     /locus_tag="ACG8OO_001802"
     CDS             complement(14424..15563)
                     /locus_tag="ACG8OO_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFP9045677.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4335 a         3425 c         2842 g         5272 t
ORIGIN      
        1 aagtcttgtc gcagtccctt attccaaata aacggtgttc aatcatctga ccctcggtat
       61 tagctcaaaa aacggcaaaa tatgaggaac tattttccat ttgtttctct aatactcagg
      121 acaaaacgct gatttcacaa cctccgatat acggtgttcc agaagtagct tcttcgaaaa
      181 ataagctgag atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatgtctt
      241 gaagctgacc acttctgtca taacctcttt ttactgttct taaacacgga taaacatccg
      301 catctcattt tatgagtgct tagatacttt gatacgattg attgctcggt gcaacgcaac
      361 agttgcccgt ttcaattcgt tcgtatcttg ttttgctttt gcttcttcga tttggcgttc
      421 tgcgcgttgt tttgcacgtt ccgcacgaga tacatcgata tcacgttcac gttccgcact
      481 atcagcaacg attgaaacaa cattatcacg aacttccatg attccgccat taacagcgat
      541 ccaatcgacg tgggtatctg aatctgttcg ttttacacga acttcatcaa tcgtcaatgg
      601 aacgatgatt ggtgcatgtt taggcaaaat cccaagctca ccagctgtcg tacgtgcaac
      661 gacgatagct gcatggtgat catagaccaa accggcagga gttaccacat ttactgttaa
      721 atattgatcc atggggcacc cctttctagt agcctagtgt tttcgctttt tctacgacgt
      781 cttcgattcg gccaacactt cggaaagctt cttcaggaag atcatcgtat ttgccttcca
      841 agatttcgcg gaatcctttc actgtttctt ctacagggac atatgaacct ggaagaccag
      901 taaattgttc agccacgtta aagttttgtg acaagaagaa ttggactctg cgggcacgag
      961 aaactaacac tttttcttga tcggataatt catccatacc taagatagca atgatatctt
     1021 gtaattcacg atatcgttgc aatacgtgtt ggacttctgt tgctacttta taatgttctt
     1081 ctcccacgat ttcaggtgct aaagcacttg aagaagaagc caatggatct accgctggat
     1141 aaatcccttg ttctgttaat ttacgttcca agttggttgt cgcatccaaa tgggcgaatg
     1201 ctgttgccgg cgccgggtca gtgtagtcat cggctggcac atagattgct tggatcgatg
     1261 tgatcgaacc ttttttcgta gatgtgatac gttcttgcaa ttgacccatt tctgttgcca
     1321 atgttggttg ataaccaacc gcagaaggca tccgtcctaa caaggctgat acttcagatc
     1381 cagcttgagt gaaacggaaa atgttgtcga taaacaacag tacatcttga ccttctacat
     1441 cacggaaata ttccgcaatc gtcaaaccag tcaaagcaac acgcatacgt gcacctggtg
     1501 gttcgttcat ttgtccgaac accatggctg ttttttcgat aactcctgaa tctttcattt
     1561 cataatacaa gtcattacct tcacgagtac gttcacctac accggtaaat acagaaatac
     1621 caccatgttc ttgggcgata ttatggatca gttcttggat cagtacggtt ttaccaacac
     1681 cggcaccacc gaatagtccg actttcccac cttttaaata tggggcaagc aaatcgataa
     1741 ctttgatccc tgtttccaaa atttctgtac ttgtacttaa ttcgtcaaag gctggtgctt
     1801 ttttatgaat ttcgcttctt tccgcatctt ctgggaaagg tgtttctaaa tcgatcgtat
     1861 ctcctagtac gttgaatacg cgacctaatg tttctttacc tacaggaaca gagattgctt
     1921 tgcctgtatc aatgacttcc attcctcttt gtaaaccatc tgttgattcc atcgcaatgg
     1981 tccggatcac tccgtcccct aattctaacg ctgcttcaag aacaactttt gatttatttt
     2041 cgtcattttt ataaacaact aatgcgttgt tgatatctgg taaggattgg tctaaagaaa
     2101 attccacgtc gacaacggga ccgattactt gaacaatctt gcctgaactc atcttttttc
     2161 ctccaatctc aatactattc tagtgccgaa gctccggcaa caatttcggt aatttcttgg
     2221 gtaatagccc cttgtcttgc acggttgtaa gaaaccgtca gctcatcaat gatattagca
     2281 gcattatcag ttgctgtttt cattgctgtc ataccagcag catgttctgc tgttttggcg
     2341 tcaacgatcg caccgtagat caaactttca gcatactgag gcaataactg agacaagatt
     2401 tcttcttttg aaggttcaaa aatatattct tgttcgaaag tagttgcttc ttctggatct
     2461 aagtctgaga ttggcagcat tttttctaca cgaaactgac tagttagcga attgatatga
     2521 tggttataac atacgtataa ttcatcgaat acttcgtttt ggtacatagt tgttgccata
     2581 tttacgattt tgcggacttc atcaaagctt ggctgatcag atagattgcg cagctcatat
     2641 gctagattga taccgcgtgc tttaaagaag tctgcaccag ttccgccaat tgcgatcatc
     2701 acgtaatcat cgggtgattg gtggtcttct tctaaaatag acatcgtttg tttcaatatc
     2761 gagctgttat atccaccgac taaaccgcca tctgcagtga tgacgatata acccgttttc
     2821 ttaacaggac ggctgagcaa catgctgttg taattgatat cccctctagg attttctgag
     2881 gcaatatcat ttaactgagt agcagtcaaa tgcgtaacga tttctcttac ttttgttgca
     2941 taaacctgaa acttctgtga agaagcttca gattttgtca acttagaagc agacaccatc
     3001 tgcattgccc gcgtgatttg gctcgttttt ttcgttgaag cgatacgtgt cttgatttca
     3061 tttaatgaag cacccattca tctcacctca ctatgcattt tgaatggatt tcaatttatc
     3121 ttctgtagag gaattatttc cttttgtagc taagaacatg tctttatatt cctgaatcgc
     3181 actgttcaac gcttcttctt ctggaagatc ttttgttgta cggattgttt caaagatatc
     3241 tgaatgattg gtatccagat attcgaataa ttcatgttcg aagtctaaaa tactgtctac
     3301 agggatacta tctaagaatc catgtgtcaa agcatacaaa atcactactt gtttttcaac
     3361 agctagcggt gcatgcaatt tttgtttcaa gatttctact gtacggcgtc cacgatttag
     3421 ttttgcttgt gttgctgcat ccaagtcaga accgaattgt gtaaatgctt ctaattcacg
     3481 gtaacttgct aagtctagac gtaaagtccc agcaactttt ttcattgctt tgatttgcgc
     3541 agaaccccct acacgagata cagatagtcc ggcatcaacc gctggacgag tacctgcgta
     3601 gaacaagtcg ctttctaaga agatttgtcc atcagtgatc gaaataacgt ttgttgggat
     3661 ataagcagag atatctccag cttgtgtttc aacaaatggt aaggcagtca ttgaaccgcc
     3721 acctaaatca tcacttaatt ttgctgcacg ttccaataag cgtgagtgta agtagaaaac
     3781 atcccctgga taagcttcac gacctggtgg acggcgaagc agcaaggaaa gttcacgata
     3841 agcaacagct tgttttgaaa gatcatcaaa aatgatcaag acatgtttcc cgttgtacat
     3901 gaactcttca cccattgcag taccagcata tggtgcgata taaagcaacg gcgctggttg
     3961 agacgcaccg gcattcacaa cgattgtgta atccattgcg ccatattttt tcaatgtttc
     4021 aacttgtgta cgaactgtag aatctttttg tccgattgct acatagatac agatcatatc
     4081 ttgacctttt tggttgatga tcgtatcgat cgcaatggaa gttttccctg ttttacggtc
     4141 accgataact aattcacgtt ggccgcgtcc gattggtaca agagcgtcga ttgcttttag
     4201 acctgtttgc attggttcgt taacagattt acgttgcata acgcctggtg ccattgcttc
     4261 tactggacga gctttgtcag taacgatttc acctagtccg tcaattggct gacctagcgg
     4321 attaactacc cgtccaatca aggcctctcc aactggaact tccatgatct ttcctgttcg
     4381 ttttacttta tctccttcac gaatggactc gaaatcgcca aggataatga tacctacatc
     4441 atttgattct aagttttgcg ccattccgta tgagccattt gaaaattcaa gcaactcacc
     4501 actcatcgcg ttttctaaac catgtgcacg agcgatcccg tcacctacat aagttacggt
     4561 accgatttct tcgactgaaa gcacgttttg ataattttca atttgttctt taatcaaggc
     4621 actaatttct tctgctttga tggccattcg attcacctac ctctttgtac tatctattta
     4681 attgattacg catattttct aattgcgtgc gaatgcttcc gtcgattact cgatggttag
     4741 cttcaacaat cgcgccacca ataatagaag aatcaacgat ttgttttaat tcgactgatt
     4801 gatagttcat tgtttttgca acattctttt ctaattgtat tcgttgttcg tctgaaagtg
     4861 gaactgctgt tgtgacactt cccaacagca aaccttgatg ttcatcatat cgacgttcat
     4921 attcttcgat catgaacaaa agatcatcca ttcggttata cgcataaaca acttctaaaa
     4981 agtttttaac gattccatca tagccactaa caagcttgtc catgatttct cgtttttcat
     5041 gaggttctag acgaacatca cttaacatat tacctaatcc aggtacttgt tcatagattt
     5101 gacgtaagct tacgagatct tgataaattt cttctgcttt atttgaatcg atcgccagtt
     5161 caaataaagc tttcccgtaa cgtcttccta ctgtatattt atctagtttc atttgaagaa
     5221 cctagacctt cgatatattg attgattaat gattcgtgca tttctggaga taattctttg
     5281 ttcaagattt tttccgcgat ttgaagagat agttctgcaa catcgtcttt tacagagttc
     5341 agcgctgaat cacgttctac agtgatatca gcttgggctt tactttttag acgagctgct
     5401 tcttcttgag catccttcaa aatattttgg cggcttaatt ctccactttc tttcgcattt
     5461 ttgatgatat cagctgcatc agaacgagag gctaacaatt gttgttcgcg ttcttgttcc
     5521 attttcgctg agttgatgcg agattgttct gcagaatcca aatcattggc gatcttgtct
     5581 tcacgttttt tcaaaatatc gctgattggt ccccaagcaa aatgcttcaa gagagccagt
     5641 agtatcaaaa atgagccgct gacaacgatg acatttccta acatcgcatt gccaacttct
     5701 gcaattgcca attgattcag cataggcgag ccttcctttc tttgaagtac ttctagtttt
     5761 catgtaaaaa agcagtaaca aaccaaattg tttctgcctt aatactgatt caattaaaca
     5821 ccaaaaacta gaatcaaggc gacaactacc cctaagatag gaacagcttc gactagagct
     5881 accccgataa acattgttgt acggaattga ccagacattt caggttggcg agccattgat
     5941 tcgattgttt tagagataac ttgaccgtta ccataaccgg ctccgattgc tgctcccatg
     6001 attgcgattg ctgctgcgat ataattcata attgttttcc tcctaaatta gataaatagt
     6061 agtttgtttt taaaacgaat attattcttc cacttcaatc ttatggctca tataaaccat
     6121 agataaagta acgaagacaa atgcttggat gcttccgata aacaaggaga atgcgatcca
     6181 gaccatctct aatggaattg ctaacggcaa tgagaaccag cctaagctta ttaccatttt
     6241 tgcaatcaag gttagcaaaa cttcccccgc ataaatattc ccataaagac gtaaagcaag
     6301 tgttaataag tttgtaaatt cttcaatcag tttcatcgga aacataaaac tatatggttt
     6361 caagaacgaa tttacgaaat atcctttgaa gcctaacttt ttcaccccaa ataagtgagt
     6421 aagtaagatc attataaatg ccaaagttaa agtaacaaac gggtctgctg tcgggctttt
     6481 ccaaagtgtt gtttcgttag gtaaaacgat ttttgttact aaaccgatgt tgtttgcaac
     6541 aaaaacaaat aaaaacaatg taaatgccaa caaatggaaa ttgctcaatt cttttctagg
     6601 cagattatct gttacgattc ctcgactaaa atcaatgacc cattctatcg cattttgttt
     6661 gcctgttggt ttcaacttaa gatttcttgt aaaaaagtaa gctaagaaga aaacaatcaa
     6721 acaggtcagt aacaccatta tacaaactgt accatcaaac caaattggtc caatgtggaa
     6781 cgttagcgag cgttcatcca atccatttca cctcttttcc ccgaaattca cataaaatca
     6841 attcatgtgc gtatctggtt ttttccaatc ggtttctaac cacacggtat cataccacca
     6901 tgaaacagaa aattcaaaac attttcatga aaattagaca cttttttatt tcagtcaaaa
     6961 acgaagcgaa tttttcttta tacacggtgc tcctgatcta agaaaaaacc tatgataatc
     7021 atgttttatc agattaaatg ctaacaaatt ttatcattaa gtaaaagcaa aagcaccgaa
     7081 tatgcgaaaa aggataatga aattatctag agaaattttt taatgttcga tcaatttgtc
     7141 gatcgatatc ttttcgtttc aaatcttccc gtttatcata agattttttc cctttcgcca
     7201 gaccaatcaa taccttggca tagccgttac gaatatagac ttttaaagga acaacagtga
     7261 ttccagtatt tttcaattca ttttcaagcc ggatgatttg ttttttgtgt aataataact
     7321 ttctcgtgcg caacggatca tgattaaaaa tatttccttg ttcataagga ctgatatgaa
     7381 cattatgcaa gaaagcttct ccgttgcgga cgcgaataaa tccatctttt agattgatcc
     7441 ggctgtttcg tatcgacttg atctcggttc cttgcaagac catccctgct tccatcgtgt
     7501 cgatgatcga ataatcatgg cgagctttct tgttttgtgc aattaatttt ccctcgcctt
     7561 ttggcatagc tgtttcctcc aactcatttt attttttctt tttcttcttt ttaacacctt
     7621 tgtaaaatgg cttcttccct tttttcttac cgaacgtttg cttactatcg ctatatcgtt
     7681 tgttcttctt gccatttctt tctgggcgtt ttcctgcttg ttttccttcg cgttttcctg
     7741 attttccatt tcgtgttttt ggccgttcca acgctgtaac ctcttctgcg gaaacaagtt
     7801 caaaatctac tgctcgttca tctggatcag ctttgactac ttttactcgc actttctgtc
     7861 cgattttaaa agtcagacca gttctttcac ctacaagagc taaatgattt tcaacaaaat
     7921 ggaagtagtc ctgtttcaat tcattgatat ggatcaatcc ctctactgta ttcggcagtt
     7981 cgataaacag gccgaatttc acaacagagc tgatgatccc atcgaattct tcccccactt
     8041 tgtccatcat gaattctgct ttcttcatgg aatctacttc acgttctgtt tctactgcac
     8101 gacgttccat cttagaacta tgatctgcaa tttcaggaag ttccgtagcc cacgaacttt
     8161 gattgctttc acttttatct tcactgtaag aacggatcag acgatggacg attagatcgg
     8221 gatagcgtcg aataggagat gtaaagtgtg tgtagtaatc tgccgctaag ccataatgcc
     8281 caaaattgtc ttctgaatag cgtgcttgtt gcatgcttct tagcaacata gtattgataa
     8341 ctgcagcttc tggtttgtct tctactcctt cgatcacatg ctgtaaatct tttggtgtga
     8401 tcgttccttt cgttcctcgg accagaatgc ccaaagctga agcaaaatca aagaagcgct
     8461 gcattttttc ttcttttggc tgttcatgga ttcggtaaat aaacggcaga tttaatttat
     8521 tataatgttc agctaccgtt tcattggccg caagcatgaa agattcgatc aagcgttcac
     8581 caattcctct gcttcttagc tggatatcat ttgggtgtcc ttcagcatca acaaggattt
     8641 ttgcttcgcg atcctcaaaa gaaatcgcgc cacgtttgat tcgcatcgtt tcaaggattc
     8701 gatgcaattc acccatttct tggaacatag gtacgagctg agcatagcgt tgcatcgttt
     8761 cttcgtcttg atcttctaag atttgattga cagctgtgta ggtcatccgt tctgttgttt
     8821 ggatcacact tgggaaaata tcatgagaga ctacttgtcc atttggatca atctccattt
     8881 cgcaactcat ggtcagcctt ggaacatggg gattcagtga acagatacca ttcgataaac
     8941 gttgcgggat catcggaatc actcgatcag tcaaatatac acttgtccct ctttcatatg
     9001 cttctctgtc caattgactg ccttgtgtca catagtaaga aacatctgca atgtgtactc
     9061 caagaaaata attaccgtta tctaattttt taaccgtaac tgcatcatct agatcttttg
     9121 catcttctcc atcaatcgta acaatgagct gatttcttaa atctctacgt ccagttatat
     9181 ccttttcgct gatttgatct ggaacttcat ctgcttcaga caatacatca tcaggaaatt
     9241 tcgtagggat accattagcc acgacaattg agaggatatc catgccaggg tcattcttat
     9301 gaccaatcac ttgcttgatt atcccttcta aactagtcgc ataccctttt tcagggtaat
     9361 gggtcagttc gatcagcaca atacttccgt caactggacg aactccctct gctgctgcat
     9421 atactttaaa ttggttgagc tttctatctt tcgggataac aacgccgtaa agatccgttt
     9481 cttgtatttc ttcttctgaa taagcaacga attcccctgc taactgagta gtcgcccggt
     9541 ctcgaatctc gactactttc ccctctgctc cgcgatccat atctgtattt gctcgcttca
     9601 tgatatcgat tgctacggtg tctccatcca ttgcaaaacc agtcgcttct tttgggatat
     9661 agatgtcatc ttcttcggga tcaatcgtta caaatccaaa tcctcgttca tttgctcgaa
     9721 atgttccttc gatcaagatt ggttttaaag gaagtttcac ttttcctttt ttattgaaaa
     9781 ctatactctg ttctctttcc atttgagcaa tcgtttgtac tagtaattta aaatcatcgc
     9841 ttttttggaa ccctaaacct tcagcgattt cttccataga aaaactgcgt ttgcgactgc
     9901 tttccatgaa aaagagaatt ttctctttta tcgtttcttt tgtcatattt attcctcatt
     9961 ccaaggtaat ttttctaaaa attccaagac gtctttttct aattcgcgtc ttgctgtacc
    10021 aactgtaatc acatgaccac tttcaggata ccatcgaagt gtcactcgct gacggctcaa
    10081 cttgcgtgca gtttcaaata cgccattagg gtcgatcatt tcatctttcc cagcttgagc
    10141 taaaaatatc ggctgttgga tatctgacaa cctactttct acgattgctg cctgatcttg
    10201 tatatccatt aattgttgtt cagctaacga gcggtaagca actgcttttt cattgatttc
    10261 tttctctgat ttacctgctg tcttcagcac ttgacgtacg tatttttcaa agttttcagg
    10321 cacgtttgtt tttacaggag acaaaggcga acagaaaaaa ccgccgccaa tcactgattc
    10381 ttcttctagt gctcgtacag caaagatgcc gcccattgat aatcctaata cggcaatatc
    10441 agaaaactgc tcagactgta aaaaatggat ggctttttta gtgtctgccc accagctttc
    10501 tgctttttct tgcaaaatat catatggctc cattgttccg tgtcctttaa aaagcggcgc
    10561 ataaactgta taatctgctt tttccaaaaa tcgtgcgagc attcgaacat cgtttggact
    10621 tcctgagtaa gcgtgaagca gcaaaaccgc tcgctttcca tgttttgcat aaaggggttt
    10681 tggtaattgt ctcactcgtc tcacctcttt cgcctcattt taccatagtt tctttcataa
    10741 cttcttctta agcgttctct ttgcaataaa aaaagccgcc cgccgttttg cgagaggctt
    10801 attcaactac ttcgaagata aatatgctag gacaaataat aagatcatcc agatagctcc
    10861 cataactgca gttgatcgct gcatgacagc ttcaaagccg cgagcttttt gtttgccgaa
    10921 tagttgatcc gcaccgccgg taaatgcact cgctgcacta ttttgcttgc ttggctgcat
    10981 cataatcgca atgaccataa caatcgataa aacgatcacg attcccaaaa ttaaattata
    11041 catatcgcag cctccttccc aaaagatatc gtctctttaa ctttatcata gataaaagtc
    11101 cgtgacaact ctatataaat ggaataacag ctgaaagttc aaaactttca gctgttattc
    11161 ttatgactga cgtaagacgt cttgatattc tggtttcttt tcaaactctt tttttgcata
    11221 agggcataaa ggaatgatct ttttcccgtc tcgtcttgct aattctactc cagtatacac
    11281 tagcttttca gctaactttt gaccacgata tgctggatct acaaatgtat ggtcaatgat
    11341 catgatgtct gggcccgcat ctgaccatgt catttctccg atctcttgtt gttcatcatt
    11401 gaataaagca agacgatttt tttcttcttt gatttccata tgccgcactc ctcaaataac
    11461 tgatattttt ttattcctac cttattttag cagtctcctg atctataaac aattatttat
    11521 cctcgaatta cttacaaaaa agaagagaaa attaattcat cgttattctt tgttcaataa
    11581 atgaaaaaat cactgtccaa taggttttcg gatctttcat cgatgcttcc aagtgttctg
    11641 cttctggaac aagcagcagt gctttatccc ctgccgtagc attttgaatc gtgtacgctg
    11701 cttctgtagg gacaaaatga tctctttcac catgaatgac caataaaggc agatggttgc
    11761 tgccaagctg tctcgtagct gatgcttgct ttaaggagta accggcgtat ttctgggcat
    11821 aatgattcgc atatctcatg accatttttt taggaaagac agtgatttta cttaacatat
    11881 atcggaattc tgcataaaca gaaacatatc cgctgtctaa aataaatcct ttgaccgctg
    11941 atggaagttt ttcaccactt gccatcatga tcgtagaagc tcccattgat ccgccgtgta
    12001 aaataatgga cgcattcggc tgttcatcta agatgagttg gatccaggca attaaatcta
    12061 gccgatctag ccatcccatt ccgatgattt ctccttcact ttctccgtgc gcacgtaaat
    12121 caggtacaag aacgttgaat cctttctctg catatctttt acctatatga gacatgtctc
    12181 ttttccctgt gcttcggtaa tcatgtacgc aaatgaccca tttattctgc ttatttggat
    12241 ggatgaacat ctgaccagct agtttcaacc cgtcataact tttgattgtt ttgggtgttc
    12301 cctgctccca aaagctttca cactcttctt ctaatttttg tcgttttttt tctactgacg
    12361 tgactgcagc agggtccatc aaacgatgac cagtgtcttc ataccagtca tatcctctct
    12421 gtactcccgc tcttactaat ttctttgcat aaaaataaga aagaaaatgg taactgatcc
    12481 ctataattat cagaataaga atgaaataaa aaataaccat tgtacacccg ccgctttctg
    12541 ccaaactata atctaactat tatccaatgt accgatccat aggattcttt attttcggca
    12601 cacgtaaatt ccatttcttt ttcattgtat catttactta aagttctcct caatagattt
    12661 acttgtaaga gagcatttac attcgcaata aaagccagta tactaatttc gtgcattata
    12721 atcaagacgc tcgtcttgga ctggttcgga aacttcccag aataaaaaac taagtatctc
    12781 taaagaaagg agattggaaa taatgcgaaa agtaattatt gattgcgatc cagggatcga
    12841 cgacacttta gctttatcat tagctgtcaa atcccctgat atcgaagtag ttgctattac
    12901 cgtagtttgt gggaatgtcc ctgcagatat aggtacgcag aatgtgctga aatgcctgga
    12961 acgctgtgat cgtttagaca ttcctgttta tcaaggaatg gaaaaacctt tgaagcaacc
    13021 tttcatcagt gcacaagaca ctcatggtat ggatggttta ggggaaacaa atttcccgat
    13081 gatcttgaga aaacaggcag aacctctcca tgccgttgat tttttggctg attatttcaa
    13141 agaaaagaca aatacatcag tcattgcttt gggtcctttg accaatatcg cttttgcttt
    13201 gaaagtcaac ccaaatattg ggaaacatat ggatcgtttt gtctcgatgg gtggaactta
    13261 caaaagccat ggtaactgtt cgccagttgc tgaatacaat tactggtgtg atcctgaagc
    13321 agcttcttat gtattcgaga atctcaaaca aacgatagaa atggttgggt tagatgtgac
    13381 aagagaaatc gtcttgacac cgactatcct cgaatactgc tgtcaaatga atcctgaaga
    13441 gggagagtat ctaaaagcga tcactcgttt ttattttgac ttccactgga aacaagaaag
    13501 aattttagga tgtgtcatca atgatccgtt agctgttgct tattttatcg agggggccat
    13561 ctgtgaaggt ttcaaaagct tcactgctgt ggaaactcaa ggaataagtc gtggacaaac
    13621 attggttgat cgttatgaat tttggcagaa acctgctaac agtaaaatta tgacaaaagt
    13681 agatactcgt ctttttttcc gaaagttttt aaccgttctt ttaaatgcac aagaagaaac
    13741 gattatgaaa gatttggaaa ggttaaagat gggctaacaa aatgaataaa aacaaaatgg
    13801 caactcgcgt acttacagtg atggctttgt gtattgggat caattatatt ggaggaacga
    13861 ttgctttatg gcttcgtttg ccaatctatc tcgattctat cggaacgatt tttgctggtg
    13921 cattgcttgg gcctgtacct ggaatgctga caggattgtc cagcggtatt ttaagtggag
    13981 tgactacaga tattttttct ctgtattatt cgccaatcca aattctcaca ggcttgcttg
    14041 ctggattgat tttgccaaga tatctcggca aagataaaaa cagcttgaaa ctgttattac
    14101 tcgctttgat tttgtcggta cctggaacga tcgtttcttc gatcattacc gttcagttat
    14161 ttggcgggat cacctcctca ggatccagta tgatcgttca gctattacat gggttaggtt
    14221 taagtcagac aacaagtgtg atcttggtac aggcaggtac agactacctt gatcgtttgt
    14281 tgtctatcgt cgttgtttca ttagtcatca ctatgttgcc acaacgcttg atatattcaa
    14341 aaagaaaaat aggctgaata aacagagctg gattagcaaa ataatgctaa tctggctctg
    14401 ttttttcatt tattaaaaaa atcttacgct aagtaggata aaataattcc ttgatatttt
    14461 tcaatcatat ccaaataatt atcgatttca acatactcat ctacttgatg aggcaaagta
    14521 acgacaccag gcccaaagac gacaaagtcg aaggaatggt tagctttcgt aaattcggct
    14581 gcatccgttg ttgctgccgc tccaactaaa ggtaatggct ggctgaattg ttgctgaatg
    14641 caatgaatca aaggagaatc tggatcagct ttgacaggta ttttgttgta atcgatcatc
    14701 aattccaaat gatagtctgt ctcttgattc aattcattga cgattgattg cagcaatgca
    14761 atgattttat catttgggta ttctggaatc gatcgaatgt tcccttggag ctgcgcatgg
    14821 cttgggatgc tgttgacttg attcccgcca ctgatcaatg taacattatg gattgttttt
    14881 cctaggacag gattctcgat tgtttcagcg aggtgattca tttcagcatt tgctttcgta
    14941 atgaattcat ttaggtgatt gattgcatta taaccttggt ctggcataga gctatgcgct
    15001 tcttttccgt gcgaggtgac tgtatagttg attgatccca tgtgcgtata catcaaactg
    15061 taattagttg gctctccgat gatcaaggca tctaagtcat ctacatagcc tgctttcgtg
    15121 agctgttccc cacctagttc acccacttct tctccgacag tggctaataa tttgactgtt
    15181 ccgttaaatg gttttcctga ctctttcagt tcgatcatgg caatcaccat tgctgccaag
    15241 ccactcttca tatctgtagc accacgtccg tacaaacggt tcccatggat ttctgctgca
    15301 aagggtgcat aggtccaaga cgattcatca ccagccgcca cgacatccat atgcccagat
    15361 aggcctaaca cttttcctga ttggcccttt tggtaacgag caatcaaatt atctctacca
    15421 tctctgtaag aaacaatctc tcctgttatg tcatgctttg ccaaaagctt attcaaatag
    15481 gctgctactt caccttcgtt tccattcact gatttaattc ggatgatctc ttgtaaaatc
    15541 gcgatttttt cttctttttt catctaatcc tctcccttgc catttctttt tattgtattt
    15601 gagtgtgctc gaattttcaa ataggagcaa ccaaagtaaa aatgaagaaa gagagccact
    15661 ttatgaaaaa tatgctgcta cttccctcct attttattta tacgagagga cttgctcata
    15721 gattcctatt ctactgttct atccttaaaa gctcttatat gatgctagtt attgttagct
    15781 ttcctttttc aaggatgatc ttttcttgta ccattctttg taaacatcat atacagaagc
    15841 tagttgcaaa aaaaactctc tacctgctga tatt
//
LOCUS       DAZKBH010000061        15688 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000061 DAZKBH010000000
VERSION     DAZKBH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.061"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..562
                     /locus_tag="ACG8OO_001803"
     CDS             <1..562
                     /locus_tag="ACG8OO_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9045678.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="ACG8OO_001804"
     CDS             762..1076
                     /locus_tag="ACG8OO_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9045679.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="ACG8OO_001805"
     CDS             1097..1474
                     /locus_tag="ACG8OO_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9045680.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="ACG8OO_001806"
     CDS             1484..2257
                     /locus_tag="ACG8OO_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045681.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="ACG8OO_001807"
     CDS             2279..3682
                     /locus_tag="ACG8OO_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9045682.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /gene="mobT"
                     /locus_tag="ACG8OO_001808"
     CDS             3864..5048
                     /gene="mobT"
                     /locus_tag="ACG8OO_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9045683.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="ACG8OO_001809"
     CDS             5045..5335
                     /locus_tag="ACG8OO_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045684.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="ACG8OO_001810"
     CDS             5332..5553
                     /locus_tag="ACG8OO_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045685.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="ACG8OO_001811"
     CDS             5595..6374
                     /locus_tag="ACG8OO_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFP9045686.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6435..6935
                     /locus_tag="ACG8OO_001812"
     CDS             6435..6935
                     /locus_tag="ACG8OO_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9045687.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6991..7629
                     /locus_tag="ACG8OO_001813"
     CDS             6991..7629
                     /locus_tag="ACG8OO_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFP9045688.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="ACG8OO_001814"
     CDS             7701..8096
                     /locus_tag="ACG8OO_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9045689.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="ACG8OO_001815"
     CDS             8080..10533
                     /locus_tag="ACG8OO_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9045690.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="ACG8OO_001816"
     CDS             10530..12557
                     /locus_tag="ACG8OO_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9045691.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="ACG8OO_001817"
     CDS             12554..13576
                     /locus_tag="ACG8OO_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFP9045692.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="ACG8OO_001818"
     CDS             13593..14522
                     /locus_tag="ACG8OO_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9045693.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14836..14883
                     /locus_tag="ACG8OO_001819"
                     /pseudo
     CDS             14836..14883
                     /locus_tag="ACG8OO_001819"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14899..>15688
                     /locus_tag="ACG8OO_001820"
     CDS             14899..>15688
                     /locus_tag="ACG8OO_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9045694.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQ"
BASE COUNT         5350 a         2600 c         3103 g         4635 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaac
//
LOCUS       DAZKBH010000062        15614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000062 DAZKBH010000000
VERSION     DAZKBH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.062"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            222..572
                     /locus_tag="ACG8OO_001821"
     CDS             222..572
                     /locus_tag="ACG8OO_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFP9045695.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            671..1201
                     /locus_tag="ACG8OO_001822"
     CDS             671..1201
                     /locus_tag="ACG8OO_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9045696.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1355..2506
                     /locus_tag="ACG8OO_001823"
     CDS             1355..2506
                     /locus_tag="ACG8OO_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9045697.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2615..4654)
                     /locus_tag="ACG8OO_001824"
     CDS             complement(2615..4654)
                     /locus_tag="ACG8OO_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9045698.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNSAQISNEDSFNSDILLADTGYGQGELLVTPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4712..5269)
                     /locus_tag="ACG8OO_001825"
                     /pseudo
     CDS             complement(4712..5269)
                     /locus_tag="ACG8OO_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(5417..5583)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(5808..6014)
                     /locus_tag="ACG8OO_001826"
     CDS             complement(5808..6014)
                     /locus_tag="ACG8OO_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045699.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(6030..6644)
                     /locus_tag="ACG8OO_001827"
     CDS             complement(6030..6644)
                     /locus_tag="ACG8OO_001827"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9045700.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6791..7474)
                     /locus_tag="ACG8OO_001828"
     CDS             complement(6791..7474)
                     /locus_tag="ACG8OO_001828"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9045701.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7583..8041)
                     /locus_tag="ACG8OO_001829"
     CDS             complement(7583..8041)
                     /locus_tag="ACG8OO_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9045702.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(8125..9888)
                     /locus_tag="ACG8OO_001830"
     CDS             complement(8125..9888)
                     /locus_tag="ACG8OO_001830"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9045703.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(10109..11569)
                     /locus_tag="ACG8OO_001831"
     CDS             complement(10109..11569)
                     /locus_tag="ACG8OO_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045704.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11777..12703)
                     /gene="glsA"
                     /locus_tag="ACG8OO_001832"
     CDS             complement(11777..12703)
                     /gene="glsA"
                     /locus_tag="ACG8OO_001832"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9045705.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12726..14153)
                     /locus_tag="ACG8OO_001833"
     CDS             complement(12726..14153)
                     /locus_tag="ACG8OO_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9045706.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14184..15398)
                     /locus_tag="ACG8OO_001834"
     CDS             complement(14184..15398)
                     /locus_tag="ACG8OO_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFP9045707.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4469 a         3117 c         2684 g         5344 t
ORIGIN      
        1 actcctttgg taggatgacg ttttggtcga ctaaatttta ccaaaagtga gtctcttttt
       61 gagttgaaac gaagaaaaag tgttagtaga aaatcgcctt cgattttcca ccaacacaaa
      121 atattataga acctttgttt ttttgaataa tcgctttttc ttctcttttt cttatatact
      181 ataagtagta aataatttga aaaaaagaaa ggtgactgat tatggcgcaa aatcgtattt
      241 ttacgacaag ttttgcaagc gtttatccta tgtatgtagc aaaagcggag agaaaagatc
      301 gtataaaaga ggaagtagat gaaatcattt gttggctgac gggttattcc caaacagaac
      361 tagaatatca actacaaaaa caagtgactt tcgaacaatt ttttgcggaa gcccctgtcc
      421 ccaatccttc acgttccttg attacaggtg ttgtttgtgg tgtacgagta gaagacatcg
      481 aggataactt gatgaaagaa attcgttatt tagataaaat gatcgatgag ctagcaaaag
      541 gcaaaaaaat ggaaaagatc ctcagaagtt gatttttcca gagatattct tctccttcgc
      601 tcattttgta acatttaatt tggtaaaatg actagttcac gttaaaataa gtcaaaggag
      661 gttttcttat atgtttgaga cgattaaaca aaatattcaa aaatacgcct tattaagagc
      721 tgctgtttat attatttctg ggattgcgat tgtgctaaac ccttctgctg tttttcattt
      781 tatcggttat ctgatcaccg cctatcttgt tttgcttggg ttgttgaatc tttttgaggc
      841 ttaccgtttt aaaaggaaaa caggttcttg gagtatcggt ctgttcagtg gtcttttttt
      901 cctgatctta gctctgatcg tctatgcttt tgcacctgca atcgtttcta tcctgccgat
      961 tcttcttgga ttagctgtta tcatcaacag cttgttccag ttgttcttca gcatgaatac
     1021 aaaagctaaa agttggagtg tttatagtag tattctgttg ttgattggcg gattcgtctt
     1081 attgttcaat ccattcaaaa gtttgatgct tctattccaa atattcggct ttattttgat
     1141 tttcatgggc atccaggaga ttatcaattt catcagaata agaaaaatga ctatacgtta
     1201 atcattttta gttattaaaa tagaccgtat aaaaaccaac aatccaaaaa aatcttttgg
     1261 attgttggtt tttttgtgag tatattttcc tattttttca ataatgtatg ttatgctaag
     1321 ttcgggctat attttataca aaggaatgat gagaatggaa tttattggta ttttatgcct
     1381 gatattgatt tcaacaacga ttggctcaca tatctcgcgg agattaggta tcccagcagt
     1441 gattggacaa ttacttgttg gtgttttgct aggccaagct ggattgaatt gggtcaaacc
     1501 tgatattcta gtccatgact tttcggagat tggtgtgatc ttattaatgt ttttagctgg
     1561 tctggaaagt gatttatctc ttttgaaaaa atatttccga cctggtatgt tcgttgcttt
     1621 gcttgggatc atcttcccgg ttgtttttgg ctggttgaca ggcaaagcat tccaagtttc
     1681 gggaaatgaa gcgattttct ttgggattat tcttgcggca acttctgtca gtatttcagt
     1741 agaagtatta aaagaactaa acgtagtgaa tacaaaagaa ggatcaacaa ttttgggtgc
     1801 ttctgtggta gatgacattt tagtcgtttt agttctaagt ttcagcctat cttttctaac
     1861 gggggaagct tctagcaatg cttctttacc gcttgttttg attgaacagc taatttattt
     1921 tgtttttatt ttcttattag tcaaatggat tgcgcctttc ctactttcat ttgcagaaaa
     1981 agtgtacgca aattcttcta ttatcatcgt atcgctagtt atttgtttag gaatgtctta
     2041 cgtagcggat cttgttgggc ttagctctgt cattggtgct ttttttgctg ggatagctgt
     2101 cagtcagaca aaggttaaag aagaagtata ccacaatgta gaggctttag gatatgcagt
     2161 atttatacca gtattttttg tcagtgtagg attagaagtt gatttttctg actttagtga
     2221 acaactactc tttatttcta tattgacgct tgtagcgatt ttgaccaaat tgcttggagg
     2281 ttattttggt gctcagattg ccggtttttc tcaacatagt tcattgatgg tcggtgctgg
     2341 tatgatttcg cgtggagaaa tggctttgat cattttacaa attggacagc aaagtcaatt
     2401 gatcgaacca cagtactatt cacctttagt tatcgtgatt ttattaagta cacttatttc
     2461 tccacttgtt ctcaaatatt ttacaaaaaa agtttatgaa gtgtgagtaa atcaaaaaga
     2521 agttgtgaaa gagacatcgt gaaaaccgtt tatttgaaaa aaggactgtg acgataacgc
     2581 cacagtcctt ttactgtact taaaaagcaa ttttttattg ataattttgg ttgaggtatt
     2641 gcaatagttc cgatgctcgt ttggttgctg aatcatcatc ttctttattt tccaacatgc
     2701 tgaccatcat ataaccttgg ttatcaagat tgaaagcaaa caagaaactg ttctcttgcc
     2761 cctttacatc ctgtttttct ttgatttccg ctgtaccagt tttcgctgcc aacggaatcc
     2821 ctaaagcaga aagagaatgt gctgtaccat ttacatcttg cacaacttct ctcagatctg
     2881 gcacgatcgt ttgtactgct gtttcgctga tgacattctt cttatctttt gtctctttat
     2941 ctgcaatcaa tttaggatag acaagtgtgc cattgttggt aaaaacagaa tacattgctg
     3001 cttgctggat aggagtaact agaagttcgc cctgtccata tccagtatca gctagcaaga
     3061 tatctgagtt aaagctatct tcattagaaa tttgtgctga attcatacta attggcaaat
     3121 caaggtcttc accaaaaatg aatttatcta agcctgtacg aaattttttc tcacccattt
     3181 tcaacgtttc ttgtgccgca tatatattat cggaatagat caaagcagtt tttaagtcta
     3241 cttgtgatac atcactactg acacgcgttg ttttaaaaga tccccaagaa ctgtcttttt
     3301 gccatttaag cccgttgatc gtcaacactt catttggatc gatagtgccg ttgtcgagac
     3361 cgattgctgc tgtaatcatt ttaaacgtag atccaggagc ataacctgtc gcaaatcggc
     3421 tgatgaatgg atgttcagga ttttcttcat aagctttgta atcttcttgt gagatcccgt
     3481 ttgtcatttt gtttggatca tagcttggag agctagcaag cgcaagaaga tcaccggttt
     3541 ttggcgttgt cgcaacagtt gatgcagctt ttcctcctag actgtcaaaa gctatttttt
     3601 gtgccttcgc atcgattgtc aatttgatat ctttgccatt ttgaacttca tgttcgatca
     3661 gaactttttt ctctacaccg tctgcatctg taatgcttaa tttcccacct gttgtcccac
     3721 gaagatcctt atcaaaagcc atctccaaac ctgagcgacc gatttttcca ttactgctta
     3781 attctggatt cttatcaata tcttctgctg tgatatctcc cacgtaacca atcagctgag
     3841 cagctgcttc acccaaagga taatatctgc cgtctacttc ttgaatggta gctccagctg
     3901 gaagttctgg cgttgctcca tcaatgattt tcaatgggac aaagtaatcg ggttgtaccc
     3961 agctttgtga aatcgcctga ttgatagcat cttctgttaa gtcgaaggag gaagcaatcg
     4021 ctttgatatt ggctgttttt tcgcctccat ctccaagttt acttggaacg actcctaatt
     4081 gttttagttt gcctgttgtt gctaatggtt caccatttcg atctataata ttccctcttg
     4141 ctgcttcttg tgtggtcagg cttactttgt catttccttc catttctgga aaaaccaagt
     4201 tgggttgcca gttgatcgtg gtttgaccat catttctgtc taatgttcct ttataagaaa
     4261 gatctttcaa ttcacctaat gaggtattca tctttgcttt atatgaaaaa ctgtaagtag
     4321 aatcgtcttt tttatctact tttagattcg atatctgaag tcctttgaca tcagcagcac
     4381 cgtatatatt ctggtattta tctaagatct ctttttcaga taatgcactt ttatttgccg
     4441 ccttttttga tgtcatttct gcagctttgt tataatctcc cttgttcaaa gcttggacaa
     4501 attgctcaac tgtcttttct ccagcttcta ctgcttgggt ttcttgatag tgctggtagt
     4561 aaaaataacc gccacccgca gctgctatta agatcactgc gccggcaata taagcgcctg
     4621 ttcgattttt gccgtgcttg tcacttcttt tcattttttc ctccttctct tgcttgtcat
     4681 tcctttattt cactatttat acctgtttat tctaacatat gtctttgagg aaatccatac
     4741 cacaatccag aagcacactt tctggaaggt tcgtttggat actaccaagt aatttcccaa
     4801 ccgctttggg tagcttgaat attgaagtta cgtctttcag ctgctgagag atagctttga
     4861 tcacttgctg ctgccttctg atcctcccga agtctccttc tttgtcttcc cgaaatcgag
     4921 catactggag taaagtatta ccatccatca cttgctctcc tttgtttata tatacgccat
     4981 ctaaatctag agatttttct gcatcgatct tgactccacc aggaaacagt tcattaacgc
     5041 aatcgataaa atcttgaaat gtgatacttg cataataggg agcttcaaat ttgaaatttt
     5101 cctttaatgt ttgatttaat aaatcaactc ccccataaga gtaagctgca tttatcttat
     5161 tcattccgta acctggaata tcgacatatg aatctctcat gatagagatc agttttgctt
     5221 gttgtgtttt ctggttgtat tgtgcaacca gaagtgaatc cgagcgacct ccaccaatcg
     5281 atcttttttc accgcatctc ctcctcacac agcaatccac ttgtcaggtg aggagcgagt
     5341 ttgtctcatc aaataagtca gtcacaaaaa tataaagaaa aggtcagaaa ccttttcgaa
     5401 caagaattct gacctagcat catctcatcg tccgagtttt agcactatac aacgtaaaga
     5461 aaacattctt agctattgga aaaagcctta atccaacagt ttctgtttta cccattggcg
     5521 actttcgacg tttctgggca acagcctatg tttgtatagg agcctcacct aacaaggtta
     5581 ttactatatt ctatttacga gttcatgata gacttttcct aatgattagg caatggaatt
     5641 tgaaatgaaa tagtgaaatt taacaatctt taatattttt tagaaaaaaa taacaaataa
     5701 gttgaaagaa tcccgttgca aattctttca gttgcaccag gattctttga taatttttta
     5761 gttttatgtg tttttttata tgttttttca cttttcggaa taaatattta tttgttaaag
     5821 taattaaaca atccgctaat cgtttgatct ggcatttcta tttccttacc atctaaagta
     5881 acagtagtaa cgacaaaatg gtcttgcaat ggcggaactt ctaagtactg cccttgcaga
     5941 aaatcattca ctgtttcata ttcttttttc atttcttttg aatctgtcga tggtttatag
     6001 cttattttta acataactag tgctcctttt tattcgggac gtgcaatcgt gaaaatttct
     6061 cctattgctg cataatcatt tcctgccaaa cggcttacag cattcaattt tcttggatcg
     6121 atccttcctg tcttatcatc atagactgtt tcatttaaat gatatttctg tacttctagc
     6181 aaaaataaat cagctgttac tttattttta ttccgtatct cgacatgttc atataaggat
     6241 gtttcaaaac gaacttttgc ttctgtcaat gctggtactg aaatgattac tgaatcagtt
     6301 aaagtcaagt cagtcaggta cagttcgctt tcctcattag gcagattggc tgctgtttta
     6361 tttacttctt ctacgttgtc ctcatcaagg atatggataa cagcttctct tgtctcaaga
     6421 atattctttg ctgtgtcttt tactgcatct tgttttctct ggatcgccaa ggataagatg
     6481 ggtcgattcg aactgacgat attgaagtaa ctgaagggtg cgatattcac tatatctttt
     6541 gctgatttag tcgttactac tgctactggt ctagggatga ctgaaccaat cagcaacttg
     6601 taattttcac gttctgtcaa ttctgaagga ctccgttcaa acatggtttc gccttctttc
     6661 tataaaattc gttaaaaagc agatcttgaa gacaaaatga aaagtaatgc ttttttcatc
     6721 ttcttagaat ctgctttaaa ctatctatca atttttcttt tttgtcaaga atctgtgccg
     6781 tgcgtgtttt ttagttattt gctttacttg cggcttcgac atatctttta gtgatcaatt
     6841 gcggacgggt gataatagat ccaactacga ctgaaaaagc acctaattca tatgctttct
     6901 ctacttgttc aggagagtca gtatgtcctt ccataattac tgggatatcg acttgttcta
     6961 tgatttgatt caataaatca aaatcaggtg tctgcgtctt tcgtgaatat tctgtatagc
     7021 ctgctaaagt cgtagacaaa agatcaaaac catcttcttg cgcttgaagc gcttcctcaa
     7081 aagtcgaaat atctgccata gctaaccgac cgtgttcgtg aatcatttgg acaagttcag
     7141 cagttgtttt ttctgtcgga cgtttacgag aagttgcatc cagtgcaatg atttctgctt
     7201 gcgtctccaa aagttcttcc acttcttttt ctgtcgcagt aataaacact tctgacccat
     7261 cgtaatctct tttgataatc cctattaccg gtaaatcaac gattcgcttg atttcattga
     7321 tatcttctgt cgtatttgct cgaataccaa ctgctccgcc ttcttttgca gccaaagcca
     7381 ttctccccat aataaaagaa ctgtgtagcg gctcgttttc caaagcttga caagaaacga
     7441 cgactccgcc tttgatttgt tccagtgtat ccacaatatc tcctccagta atgttatata
     7501 aaaatacagc ttcatataaa aatgtaaccc ttttctattc aggagacaat aatgagctat
     7561 ttctttcagc agacctttct tactaaaaga aaattacttc aaattcttcg tatacgacca
     7621 gcttctcttc tgtaaattct atgttcaacg cttctttata agcatgttta aaaaactcag
     7681 catggacttt ccgccaataa gctaatgtcc gatccccttc tccttctttc caagcatgct
     7741 ctttcgtgac ttcactaaaa ggcaagatgg tcacatttat atttttgatg atacaaactg
     7801 gttcattttt tccattcagg accacgtcta attgaccaac ttttggtaaa ggctcattct
     7861 cttgtttata cagatcgtaa ccacttgatg taccagtctt tatctttttt acaactagtt
     7921 cgctcaactc gtctgccatt ttttctgtat ccccaaatga aaagaaacct aatttttctg
     7981 attgataact aggatatttt tcttggaact ttttgacata ttcttctact tgctgataca
     8041 taacatccac ccctttgctt tttataatga aaaaaccatc cagatactag tataaacaat
     8101 tatctggatg gtgagcataa tgaattattg aatattcaag acacgcagac tatcttcaac
     8161 gatttgtttt ggtgttaaat gataacgttc ataaagctgc cctacaggaa cgctatccgt
     8221 gaattctttc tctgcaccaa aatttaatac gttcattgaa gaatcaccat aataacgact
     8281 gatattttcg ccaaatcctc ctgacaaact accatcttct aatgtgatga ctaattcgtg
     8341 tgtttctttt aatttatcta atgtttctac gtcattttct gtgatcgttc ctggattgat
     8401 caacgttgct tcgattcctt tttttctaaa agcttttact gtttcttttc ctaattcaag
     8461 aaaaccgcct aaagctaaaa ttgctacttt tcctccatta tttactgttt gataggctgg
     8521 aatactataa tcatttgcga tagctgtacc atgttctaca ccatgtgtag gaattcggat
     8581 tgctacaggc tgcgctgttt gttcgaacgc ccaatctagc atagagatca tatcctcttt
     8641 tgttgctgga gcaagatatt tgatgttcgg gatacttgat aagtaaggaa tgtcaaatgt
     8701 tccttggtgt gttcggtcac tagctgagat cgttccgcca ccgactaaaa taatcactgg
     8761 attctcgttg atcgctaaat catgtgataa ttgatcatat gcccgttgca agaaagtact
     8821 tgattggaag acgactggac gtactccctg agaagccaaa gcagaagcga aagtgataga
     8881 gtgctgttca gcgatcccca cgtcaaaata atgatctgga tgtttttctt ggaatgtttt
     8941 cagtccaaag gcgcctggta tcgctgcatt gactgcggca ataggagctg tacttgtttg
     9001 cattttctta tccaaataat ccatgatgat catattaaat gtttctccag aagttacttg
     9061 ctttgcctcc ccggtggaca gatcaaatgg tacatgccaa tgatatttca ttttattatc
     9121 gattgccggc tgataaccgc gacctttttc cgtatggacg tgaagtacaa tcggatgatc
     9181 aatatctttt acttcttcaa ataaacggat caccgtttcc agatcgtttc cattatccac
     9241 gtaacgataa tcaaatccca ttgctttaaa taggttattg ctgcttttac cgttggtttg
     9301 acgaagctca gctaagtttt tatataaact gccatgattc tcatcaatag acatctcatt
     9361 atcgtttact aatacgatta gattggattg taaaacacca ccattgttca atccttcaaa
     9421 agctaagcca ccacttaggg agccgtcacc gataaatgct accacatttc ctgattcttt
     9481 cttcagatct ctcgctttag ctaaacctgt tgctaagctg atagatgtag atgtgtgccc
     9541 cactgtaaaa tagtcatgtt tactttcatg ttggttggaa tatccgctca cttcacggta
     9601 atgtcctttt tcaaaaccat tttttcgacc agttaaaagc ttatgaccat aagactgatg
     9661 ggaaacatcc caaacgattt tatcctttgg tgaatcaaat acataatgaa acgcaatcgt
     9721 taattctact acacctagat tcggaccaac gtgcccacct acatcgctgt ccttttgcaa
     9781 gattttttgg cgcatctctt ctgccaactg tttcatttcc tcaacagata attttttgac
     9841 atctgcgggg ctattgattg catccaatac atgtaatact gtactcataa tacattctct
     9901 cctccgtact atcttctttt aatgaaaata gatttgttac actttatcta ttctactact
     9961 tagagttgac tctaagtcaa tagcatttta aaaaaatttc agagaaagct tgttatataa
    10021 gtagatcttc gcacgaatca ggactcaaat aaaaaccttc tacagacaat taaaaagctt
    10081 tgtagaaggt ctcctaatta aggtctcatc aagaaatatg tttttttaat aggtgaagtt
    10141 cctgagcaat atgatacaaa tggatgtttg caccttttaa tgagattgga tagatctttg
    10201 tcttttcagt ttctaatcca ttttgatagc tagcagcatc gcacaaaacc actctttttt
    10261 tcgcacatag acattcatca ggcaattctt ctaatgaacg aaataaaata ggatttattt
    10321 cagcgatgcc ttcttccgca tacaaatgaa attgggttcg ataaaaaaat tgtttgactg
    10381 aactttctga aacaacaaaa atttctattt cctgtctccc cgcttgttca ctgttgatca
    10441 aagaaagact tttgacgaat tttcgaatga aattttcatt ccatcttagt tggatttgat
    10501 ttttcttttt ccactcttcc aatatttcta caatttttcc gtacagaagt tgttcttttt
    10561 ctgatagaag atagatcctt tccggtaaaa atacttgtcc atctgcccac aacgtacgga
    10621 caaattgtaa aaaggattgc ttgaaaataa aattttctgc atcgtcgatt tcagcgatac
    10681 taataatctg taaaatcaat tcattcagtg ataggtcatt gagaacaaaa tttttataaa
    10741 caacttcgaa atcttttctt aacagctcca tgttctcatt ggtataattt ctactgttga
    10801 acaaagaaaa aatataatag acatcttctt cttgtaagtc tagaattcct ttttgctctt
    10861 ccaatccttg acagactaat tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt
    10921 ctttcagtcc aaggggaaat gcctttcttc cgattgcgat ctctattcct cgtatgataa
    10981 aggacttttc atcttccgaa tatcttcttt taaaaaaatg agtctctaca taatcaatca
    11041 actcgttaca agcttcttct acatacggat ttttattttc atagccttcc caattgacat
    11101 aacgtactaa agctagcaag atgttccgcg agtcaaattc tgggataatg atttctttat
    11161 ttttcaaata atcattttct ttaaaaaaat ccaatacgat ttttttgatt gtatatactt
    11221 tgctcaaaga aataaattca agatcagcca gttctgtact cgtgaatata ccattaaaat
    11281 aataacttaa aaactttaaa aaataagatt gaccgtacag aaactgtgtg agctcagaaa
    11341 gagcaatacc atgtttgaat acgagtttat acttatgctt ctctcttact tgttctttta
    11401 tacaatattc tagacactcg gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca
    11461 cccttagaga aatagctgtt tgttcgatgt ctatttcttg gaattcaaat aattgttcac
    11521 acagatacac ttttctaaat atatttcgct cgatgtaatt ttccagcatt cctgatacac
    11581 gcctccagtt tttcttatga ttttatttaa tcatatctgt aaatcgtatt tttgtttcct
    11641 ttttatttag aaaagccctt tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa
    11701 caagctcaca atttcagcct cattgtaggt agctttgagc cgtttggact tttaccccag
    11761 cctctttcaa gaatggttat tcaaagatgt cagcgtctag ttctttgact acatctttca
    11821 acaattggat accagctgcg ctgttaccaa atggatctag tgccgggctg aagacaccta
    11881 tgccgtaacg atttggcaca gctgccatca acccgccgcc tacaccgctt tttgcaggta
    11941 caccaacatg gacggaaaac tctcctgatt catcatacaa accagcagtt gtcataattg
    12001 atttgacgat tgtagcactt tcttcagtaa tgatttgttt gccattccaa ggagcaattc
    12061 ctttatttgc cagcacagca gctaattttg ctatgcctgt agcggtgaca ttgagggagc
    12121 attgtctgaa ataagcatcg agtatctccg gtacgtctcc ttcgatcatt tgattacctt
    12181 tcatatagta agcaagagag cgattgatat cacctgttct tgattcagaa tgatagattt
    12241 cttcattcaa tgtgatatta ggatcatcac agatctcttt cataaatgac aagattcgct
    12301 caaatttttc atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat
    12361 taacaaaagg attcattgga tgtttgcgat gattgatttc catattcatg atagaattga
    12421 aggcaaaacc ggttggttcc gtgttgattg tttgaaagac tttttctatt ccgttgtctt
    12481 tgattgctaa taacaatacg ggaacttttg acatgctttc gatggcaaag cgcacatcgg
    12541 cgtctcctcc ctcaatcagt tgttttgtat ccaaatcata aatcgccata cctaattgtt
    12601 tgggatccac ttcagctaat gctggaatgt aatccgccac ttgtcctgtt tgataatagt
    12661 ggaaattttt tccaatcaat tgttctaatt cttttacttc catttgaata accagctttc
    12721 attttttatt tttctgcatg ctggaattta tgacggcatg cgtagatcgt aaatggaata
    12781 actaagacga taagataaga aacaatcaga atcgtttcat aaatcccatg ctgtccagct
    12841 gcgatactag caggtggaac aaatgagata caaaaagcaa aaagtgaagt caaaagacct
    12901 aaacaagcaa cgattacttt tacaactttt ccgcctggaa cctgataggc tctttcaaga
    12961 ctgtcatctt taaaaaccag cttcaaatag cccgcgaaaa acataaagta caacatcagg
    13021 tagattacaa cagtcaatga catcgctgca agaaatgaaa gattattccc cccgccgctt
    13081 aatgtaaaga cggctgccca gattgttgca acgatcccat ttgcgatcat caaatgagca
    13141 ggaacaccgt gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct
    13201 acgtgaagtg cttttgaagg agaggttacc caagaagcaa tttctccgat gacacctaaa
    13261 caaacaagaa tcgcaagaat tttcgcaatc catgcaagat ttggatcaat gtgtcttaat
    13321 aggaattcta acgattgaat cacccctgta ttaagagaca aaccatgttg aggtactgta
    13381 gtagcgacgg tcaatccgcc aaaagtatct aaaatgattg ccagaataac caatacaaac
    13441 attgctaatg gataatcctt tttagggttt tccatttcat ttgcatgact ggcagatgtt
    13501 tctacgccca tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa
    13561 aaatcaggaa tcaatgtttt catagacagt atcgtttgta gtggagcgcc tgaagcgaca
    13621 taatggatcc ctaagccaaa caagactaat gctggcaaca agataccagc tacaaaaccg
    13681 atccgagcaa tcaatgaagt gtatttggtt ccttttaact gtgagagtaa aatagcccag
    13741 aaaataatca ataggattgc caatttcaga tatttattgt tttgtatagc accaataccc
    13801 gttgcatagg ataatgcacc agttaagaaa tacagcatgg tcaagtagcc aactgttatc
    13861 tcaaaccatt ggaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc
    13921 caagtataga caccgccttt ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat
    13981 gccacaggga taaaccatag caaaccgcct aataacaaga aaaatactaa gctgaagcct
    14041 gaggtcgcga atgtgggata ttcatataat gaaacgacca ttgagatggt catagccaaa
    14101 aagccaaaga acgttaattt ttttgcagga attttcgttt tgctttgttg catgaatctt
    14161 cctcttttct atttgaattt gtttcaattg agtttgttgc caaagaagaa aaagtccaat
    14221 ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa
    14281 atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat cacttgaatc
    14341 cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc aaagatccct
    14401 atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc aagtgcatca
    14461 tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc cgcaatgatc
    14521 ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat ggctgccaat
    14581 ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt tacttttgca
    14641 atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc aaatgcattg
    14701 actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca gccaaaccaa
    14761 agcagtcctg taccgatagc agctgccata agactgctat gctgacttct ttctcctaaa
    14821 ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc agctgtcgtg
    14881 tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa tccgccaccc
    14941 catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac aagtataata
    15001 tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg
    15061 ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg
    15121 ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc aataacaccg
    15181 ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat cgttgttatc
    15241 ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg tgctaatccg
    15301 ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca taaaatcatg
    15361 aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc ctttctacga
    15421 ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa ttaatacttg
    15481 catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc
    15541 acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt tattttcgtc
    15601 tttttttatt ttta
//
LOCUS       DAZKBH010000063        15480 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000063 DAZKBH010000000
VERSION     DAZKBH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.063"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..188
                     /locus_tag="ACG8OO_001835"
     CDS             <1..188
                     /locus_tag="ACG8OO_001835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045708.1"
                     /translation="SIKVHLKKSVRTKVLFVNLKIDFPEYVLGISKCLNVFLNAKSMQ
                     KILYFSTFLSLTKIKKR"
     gene            complement(234..600)
                     /gene="ssrA"
                     /locus_tag="ACG8OO_001836"
     tmRNA           complement(234..600)
                     /gene="ssrA"
                     /locus_tag="ACG8OO_001836"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(771..1544)
                     /locus_tag="ACG8OO_001837"
     CDS             complement(771..1544)
                     /locus_tag="ACG8OO_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9045709.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1688..2038
                     /locus_tag="ACG8OO_001838"
     CDS             1688..2038
                     /locus_tag="ACG8OO_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP9045710.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2019..2735
                     /locus_tag="ACG8OO_001839"
     CDS             2019..2735
                     /locus_tag="ACG8OO_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFP9045711.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2735..4183
                     /locus_tag="ACG8OO_001840"
     CDS             2735..4183
                     /locus_tag="ACG8OO_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9045712.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4253..4762)
                     /locus_tag="ACG8OO_001841"
     CDS             complement(4253..4762)
                     /locus_tag="ACG8OO_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045713.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4752..5477)
                     /locus_tag="ACG8OO_001842"
     CDS             complement(4752..5477)
                     /locus_tag="ACG8OO_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045714.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5700..7820)
                     /locus_tag="ACG8OO_001843"
     CDS             complement(5700..7820)
                     /locus_tag="ACG8OO_001843"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9045715.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8050..9228
                     /locus_tag="ACG8OO_001844"
     CDS             8050..9228
                     /locus_tag="ACG8OO_001844"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFP9045716.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9244..10002
                     /gene="aroD"
                     /locus_tag="ACG8OO_001845"
     CDS             9244..10002
                     /gene="aroD"
                     /locus_tag="ACG8OO_001845"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9045717.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10025..10510
                     /gene="ybaK"
                     /locus_tag="ACG8OO_001846"
     CDS             10025..10510
                     /gene="ybaK"
                     /locus_tag="ACG8OO_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFP9045718.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10581..11834)
                     /locus_tag="ACG8OO_001847"
     CDS             complement(10581..11834)
                     /locus_tag="ACG8OO_001847"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9045719.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11952..13301
                     /gene="gdhA"
                     /locus_tag="ACG8OO_001848"
     CDS             11952..13301
                     /gene="gdhA"
                     /locus_tag="ACG8OO_001848"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9045720.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13413..14759
                     /locus_tag="ACG8OO_001849"
     CDS             13413..14759
                     /locus_tag="ACG8OO_001849"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9045721.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15067..15453)
                     /locus_tag="ACG8OO_001850"
     CDS             complement(15067..15453)
                     /locus_tag="ACG8OO_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9045722.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5055 a         2766 c         3128 g         4531 t
ORIGIN      
        1 tcagcataaa agttcacctc aaaaagagtg tacgaacaaa agttcttttt gtaaacctta
       61 aaatagattt tcctgaatac gtattgggga tttctaaatg tttgaatgtt tttttgaatg
      121 caaaatcaat gcaaaaaatt ttgtatttct ctacttttct ctccttgacc aaaataaaaa
      181 aacgttgatt taacaacgtt tttcatacat gtttatctgt ctcaaccata catatatgga
      241 gacggcggga gtcgaacccg cgtccaaaca catcgccact taaaaatcta cgttcatagt
      301 caactcattt aaggattcac tttacagctt gccgagtgac aggcattctg tatcgctagt
      361 ctgatgttct ctttttcaac ttacagacgg aaagttgaaa cgtatcccac taaagttagg
      421 acccttactc gagcacatgg gcgatgccga gaggatctac gctaactgtt tttaggcagc
      481 taaagcgtaa gagttgtttt cgtttttagc agttatattt aactgtaacg tttttacgta
      541 gacgtaacct acgaaacgca attcaagctc gactgtgcct gtcgaatccg taacgtcccc
      601 gttagctaac aagggttacg tgtatttttc atacaaaatt agtatagcat atattgggat
      661 aaattcaata aaaaagcgaa tcatatccca ttatttatat aataactgcc cctttcttaa
      721 gaaaaaataa gcgattttct taagaaaaga ggtagcttca aaaaaagatc ttaaatcaga
      781 tcaaaatact tcaatgcgtg gacaattccg ccttctgtat ttttctttgt gataaaggta
      841 gaaagttctt ttaatccgtc ataggcgttg cccatagcga ttttgttgtc gcaagcttcc
      901 aacaaggcaa aatcattcgg accatcgcca aatccaaaag taggaacttt ttccagctga
      961 agttcttttt gcagacgttt gacacctgcc ccttttgaga ttcctttctt cactgtatcg
     1021 atcgagtaag gaccattccg ataaaaagtt agctcaggaa aatgttcgtg atagtattcg
     1081 tcacctgatt gagataagat cagcaacata tttactgtgt tttctttaaa gcctaaagga
     1141 tcaatttccg gaacatctga atgaatgaag tcgtaagcat tgatcattgt ttggctatga
     1201 ccggtacacc agattttctg atcgttatag taagaaagtt catgaccgtt caactgtaca
     1261 tgatcataca ttttttgaca ttcttctgaa gaaaatgttt gagaatagat ttcttcgccg
     1321 tctactcgaa tgaaagaacc attcatggtg accgcagaag taatgcccgc atcttccatg
     1381 atagagtgga tttctgcttc tgtacgtcct gtagcgatga cggggagaac attgttttct
     1441 tttaatgcgt tcatagcttt tgccacttcg ggtgtgattt ttgatttcgc atctagtaat
     1501 gttccatcaa gatcaaagaa agtgattgca cgataatttg acacaaataa aactcctttt
     1561 ttattggaat aagtttatct taacaaagaa tgaacaaaag acaaaccttt cgtgaagaat
     1621 tcctgaacat attggttcag tcaacaattt agagtatact ataagaagag aatttaggag
     1681 ggaaagaatg atttttttca gtattggtat cttattctta ataattggca gtctttttcg
     1741 gattctgccc tcaaaaggaa atctgccttt ttatgggtat cattcgcctc ttgccgcaaa
     1801 aacagacgct cattggcggc tggctcaaaa gacaagcgga aactggtttt ttcttatggg
     1861 cttactgatg gcattgatcg gctattattt aaagacatct gggcatacga attattttct
     1921 gattgaaatg cttctgctag tatttccaat tatgcctatt tttattatga cagaaaagaa
     1981 attacagaaa tatgacctgg aaactggagg gaacgataat gaatatttta atgattgaag
     2041 ataatcaatc tgtttctgaa atgatgcaga tgtttttctt aaatgaaggt tgggaagcaa
     2101 ctttcaagta tgatggaaaa gaagggctcg atgccttttt agaaaaccca caacattggg
     2161 atatgattac actagacctg aatttgccca cactggacgg tatgctagta ggaagagaga
     2221 tccgcaaagt ttcaagtact gtgccgatta tcatgctgac agctagagat tctgaaagcg
     2281 atcaggtgat cggcttagag atgggggcag atgattatgt cacaaaacct ttcagtcctc
     2341 tgacgcttat tgctcgaatg aaagctttgc atcgccgtag tgagttggca gaagcaagag
     2401 aagaagcagc agtttcagat gatcattttg atatcgagac tagtcatttc aagatgaata
     2461 cgaaaacacg agaagcgtat ctggacaacc ggccaattga aggactgacg cctaaagagt
     2521 tcgatctatt gtttacttta gcaaaaaaac cacgtcaagt attctctcgg gagcagttgc
     2581 ttgaattagt ctgggattat cagtactttg gtgatgaacg gacagtggac gcccatatca
     2641 agaaattacg acaaaaaatc gaaaaggtcg gaccacaagt catccaaact gtttggggag
     2701 tcggctacaa attcgatgat tctggtgttg cctaatgcgt tatttgtatc agcaactctt
     2761 agctttttgc ggaatggtcg cgttgatctt attgatcgtt ggagtttctt ttacacaact
     2821 gacaaaacaa acgattgaag aaaacaatta ccagcaactg tttggatatg ctgaatcagt
     2881 tgaaaagaca acgcaaacct atgccgatac gctcccgcag tttagtcaag atcaggcgtt
     2941 tcaaaatgca ttgctgctga cagagcaagt actgacagaa caaaatgtga actttatatt
     3001 tatcgataaa aacgaacgtg tcatttatcc gactaataca gctttactga attttacctt
     3061 aacagctgat caatggaaaa atctgaggaa tggacgtcaa gaaaaattca catcgaataa
     3121 aaatatttta ggacaagcac aggcgacctc ttacgcactt gtgccattta atttgaatca
     3181 tgaattctat ggagcacttg tagttagtca acctgcgcga aatatcgata acagcgttcg
     3241 tccaattaca ctgaacctgt tcaaaggatt tattttttcc agtatcgttg cagttattgc
     3301 cagctatgct tttgcagcat tccaagttaa gcgtatcaat cggttaagga atgcgacgaa
     3361 agaagtgacg aatggtaatt ttgatgtaca gctccccgta catgacaaag atgaatttga
     3421 tgagttagca gatgatttca ataaaatgac gaattcattg aaagaatctc aagcagaaat
     3481 tgaagaacaa gaaaatcgtc gaagacaatt tatggcagat gcttctcacg agatgagaac
     3541 acctcttacg acaatcaacg gacttctgga agggttagaa tacaatgcaa tccctgaaaa
     3601 tcagcgagaa aacgcgatta aattgatgaa aaatgaaaca gagcgtctga tccgtttggt
     3661 gaatgaaaac ttggattatg aaaaaatccg tacgaatcaa atcagtatgg tcattaaaaa
     3721 attcaatgga acagaaacat tgaaaaatat tgttgcacag ttagaagcta aagcagaagc
     3781 agcaggagat acattgacat tgaaagccgt tgatgatatt gatgtttacg ctgattatga
     3841 ccgatttgtc caaatcatgg tcaatattat tcaaaacgcg atacagttta ctgaaaacgg
     3901 ccaaatcacg attactttag aaaaaggcta tcttgaaacg atcatcacga ttgaagatac
     3961 gggaattgga atgtctgaac aacaaatgaa aagtatctgg gatcgttatt ataaagtaga
     4021 tccttcaaga aaaaatacaa aatatggtga atcaggatta ggtttaccaa tcgtgcagca
     4081 acttgtacgc ttgcataaag gaaaattaga agtagaaagc gaactaggaa aaggaacaaa
     4141 attcgctgtt cgtttgcctg acgtagaaat caaaaaagag taaaaaacaa gcggtataaa
     4201 agatcatgaa tcgatcttct ataccgcttg tttttactat aaaatgattg atttagcccg
     4261 cgggctctaa acgatcaata taaggacttt gagcaatagt agcatcatga gtaaagagta
     4321 agataccttg attttcttca tgagctaaat aagaaagcaa gtggagtagc tcttggatct
     4381 ccaatacact aagttcatca aatgtatctt caataacaat attgtttcgc ttggaaagca
     4441 aggcatgaag taattgcagc tttacttttt caaaagacgt caactcagct gccgcacaat
     4501 taagcaagga aggagagata tcaagttttt cttgataggc caaacgatct tgtttgattt
     4561 ctttttccgg tacatccaac aggacatggt ctttaagatt cagataagga aacatcggcc
     4621 aatttttttg gatgatcgca aaattatcca gttttgcaac aagaacctct tttttcaagt
     4681 gctccaatat tttatgctgt ttttcgagag gacacaaaat aacggtcaag tgttcagcat
     4741 gaatcaaata ttcaggtttc attacgtcca ggcctcctac tttgcaaatg atggataaaa
     4801 aaagtgaaag ggaatagact aagaatgaga cagcatccac tgagcccaat aagagtttgg
     4861 gcaaatccct taagtatatc tgtgaaaaaa agatcatttt ggaaatcgat ggtaaaaaaa
     4921 gtctgttgat ccatcggaat tgtaagtccg cttaccgaag tcaagatatc acggatatct
     4981 ggcagttcag tagtctttaa gacaagaaaa ttgatccttt gaagaagcgc ttcataaact
     5041 gactggaagc ttacgatcca caataaaacc aagatacaac aaagaaagag aggaaagaac
     5101 agctgccaga aaataaaaag gaataatttg gttttagaaa gaccagtcag gcgccaattg
     5161 atcaattcct gcttgttttg cctgactgaa tagtaaaaga aaaagataaa aaggaaggca
     5221 aatagaaaaa ataaactgaa tatgcctttt atataaaatc ggctagctaa ttctaaagcc
     5281 ggaagaagct ctgtagatgc agaagcgcca ataaatgaaa gccgatcttg gacttgattg
     5341 gctaaagccg attgcaaatg gtaaagagtc aggcatgcta tcaataaaaa agcagaaaag
     5401 cttgaaaata aacaatagag tattgtctgc tttttgtaat aaattaatcg agcagtagca
     5461 tagcggatta ttttcatact tttcacctct ataaaaagta tagaaaatga atatgaataa
     5521 agtatgaaat tcctttagtg aaatcacgaa ttaaaaccag aaaatatttt gtaaaaagca
     5581 agaggttgtg aagaagctgc cctattctat taccgtagat gcaagctttt gaacactgtt
     5641 tattcgaaaa gaggagcagc gacaataatt ttgtcgctgc tccttttaaa tttattttgt
     5701 cattcggaag aacttgtggt cgttccgtga agttcttggt acgtttgcgt tttataaaga
     5761 tcaacagttg attgtccgtt gtgttcacta aagacactag ttgacttgtc accaagagat
     5821 ttttcagctt cttttaattt ctctaagcca ttcttgtagt cataatcact ggtatcaatt
     5881 gggtctaatc cactattcgt atagaaccga agaagatcac cattattgat ttggtcagaa
     5941 acagctaact gtttatcaac tttttctttc agtgaatcaa tttcattctg tacttcctca
     6001 gtaggttcag taatagcttc accagtttcg ttactataga tcgtaccacc gtagtaagta
     6061 tattttggtg taacaaaatc accatcacgg aaagaaacaa gctgttcatg atctttcgag
     6121 aatagatctt gtcctaactg aatataattt tttgtatcta cacctagtaa gtgaagtaat
     6181 gtaggcaagg catcaacttg tccgccgtaa gtgtgattga ttccgccctg tgtttgtcca
     6241 gggatatgga tcatataagg gacacgctgc atttgtgcat tgtcgtagtt tgtccaagta
     6301 gaacttgttt ttccaactaa ttcagctaga tttttatttc ttgaattcga gacaccgtaa
     6361 tggtcaccgt aaaggacgat gactgaatta tcatacaagc cgctttcttt caaataattg
     6421 aagaattctt gtacggcggt atctaaataa ttggctgtcg caaagtaacc gttgatcgtt
     6481 tcatcatttg tattggctaa cgggaatcct gcttcgtcat tggtgaattt tgaataagga
     6541 taatggttag atacagcaat gaattttgaa taaaatggtt gttgcagacg ttccaagtat
     6601 tgtacggatt gttggaaaaa tggtttatca tgcaacccgt attggaacga attgtcatca
     6661 ttaacatcat aataagaaga atcaaagaaa taatcatatc ctaaattctt gtatgtctcg
     6721 tttcgattcc agaatgtacc agcgttccca tggaataccg cactagtgta accttgtgtt
     6781 tgacttaaaa tatcaggtgc agcttggaaa gtattttttc cgcctaattg tgtgaacaag
     6841 gagccttgat tcaagccaaa caatgaattt tccatcagcg tttccgcatc gcttgttttt
     6901 cctgctttta cttgatggaa aaagttgtca aaactgaacg tgctgttgct gtggtaaaga
     6961 ctattgatga aaggtgtaac ttcatgctgg agaccattct catctgtcaa tttataatcg
     7021 atcaggaatt gctggaagct ttctaaatgg atataaatca cattttttcc tttagcgagc
     7081 ccgaacaaat cgtcatttgg tgctgcataa tgttctttta cataattttc tacttctttc
     7141 atatcattgg gacttgcttc tgcccgcact tggtttgtct ggtaagtttg aacaccgtca
     7201 taaaccatga aagcattcaa gccaagatat ttgaccaaat aatcacgaga gaatgttcga
     7261 gtcagcaatt ctggacgatc agcttccgcc ataaaaagat ttcctgagaa aatcataact
     7321 gctaatgtag agatcgcaaa agccattctt gctcggatcg ggcgagggtc caacttgatt
     7381 ttttttgcaa tcagcagaac agtcaagata ataaaatcga tccaatagat cacatcatat
     7441 gggcgaaaca gtcgaatggc actttctccc agaccactcg ctactttccc agcacctaaa
     7501 atcgtgttga tcgtaatgaa gtccgtgaat tctcgataat aagtgacatt cgaaaaaaca
     7561 agcaatgtca aaagaaaata aatgaccatc atcgtgatat aagcaactct agggcgtctg
     7621 atataaagag caattccaag taataacaat gtagttgcaa tagggttgat aaacaagata
     7681 aagtattgaa tgccactttc gattccgaga tggaaatcaa ctaaataggc aaaaatcgtt
     7741 ttcaaccaaa gtaaaacgac tagtaaagaa aaaaatccga gcctcttatt caaaaaggca
     7801 ggtgttttta tacatttcaa gataatcttc ctttctgcac ttcttaatca cagaagtgtc
     7861 atgaaaccat gttacacagc tcacttattc tcatttattt tactagtatg acaatatagt
     7921 gtcaattgaa accagaagtt tatgctgttt cattaaggtt ttctttaagc ttcatgaaaa
     7981 aatgagcagt aagacaagtc ctatgttttt tgttatactt gggaagagaa agctaaagca
     8041 ggtgaaggaa tgaaagtaaa agtaaaaaaa agagcaagca atcgattgaa aaaaggatat
     8101 ccactgatcc aaaaagaaga ccttgctcat ccccaaaaag aaaccacgca atgggtggaa
     8161 ttagttgatt cgcaaaacca atttatcgca aaaggatatc taggaaagca gaataaggga
     8221 atcggttggg tattgagcca gcagaacatc ccatttgatc aatctttttt tgaagaattt
     8281 tttgtgaagt caaaagaaaa aagaactttc tttttttcag atgattctac taccgctttt
     8341 cgtatattca atggagaagg agacggaatc ggcggtctga ttgttgatta ttatgcaggt
     8401 tatgctgttt tttcttggta taacgaaacg ttatatgcac atcgtgaatt attgattcaa
     8461 gcttttcgtg aaatctaccc ggaaatcgta ggaggctatg agaaaattcg atttgaatca
     8521 gtaaatctgc ctgagagcca gttcatttac ggaaaagagg caccggaacc cttgatcgta
     8581 aaagaaaacg gagtaaaatt tgccgtttat ttaaatgaag gattaatgac tgggatcttt
     8641 ttagaccaaa aagaagtacg gggatcatta gcagaaggtt tcgcttctgg gaaaacagtt
     8701 ttgaacatgt ttagttatac cggcgccttt tctgttgctg ccgcaatggg aggggcaatc
     8761 gaaacaacaa gtgtcgatct agcaaaacgc agtttaaaaa aaacacagga acagtttgaa
     8821 gtgaatggtt tggatgtcga tcagcaaaaa atcattgtta tggacgtatt cgagtatttc
     8881 aaatacgcca aaagaaaaca atttttatat gacatgattg tattggatcc accaagtttt
     8941 gccagaaata agaaaaaagt ctttcgcgta gcaaaagatt atggggaact ggtcaaggat
     9001 agtctggata ttttagcaga agacgggata cttattgcgt ctacaaatgc tgcgaatgtc
     9061 tcgatagaac aattccaaac aatgattgaa gaggaattcc atgccaaaaa tgtttctttt
     9121 gaacaacttg ctttatatcg tctgccaaaa gatttccaga cgacggatac ctttttagaa
     9181 ggcaattatc tgaaagtgtt tgtttatcaa attaaaaata accattagaa ggagcgatgc
     9241 agaatggttt gggtaaaggg attgaatata ggaagcggta gaccaaagat ttgtgcgcct
     9301 cttatcgggg ccgtccgaga ggaagtatta cgtgaagcag ttcttgcacg tgaagcaggg
     9361 gccgatttag tcgaatggcg agtcgatcat tatcgtgaag ttttttcttt tgatgaagta
     9421 gcagaaacat tggttcttat ccatgagatg cttgatggac tgccgttatt atttacgttt
     9481 cgtaccttag ctgaaggcgg agaacaagac atatcgatcc gtcagtatcg cgacctttat
     9541 gaacatgtga tacacacagg attagtagat atggtcgata tcgagttgtt caaagtagaa
     9601 agtctaggaa aaagactgat agaggaaatc aagtctttaa acattccttt aattatcagt
     9661 agtcatgatt ttaaagaaac accagcagac cctgtcttgc tttaccgatt gaatgttatg
     9721 gaacattttg gagccgatat cggaaaactg gcagtaactc ccaataatga acgagatgtt
     9781 ttgcggttga tggagttgac tagacgggca aatgcttttg tctccatgcc tattattacg
     9841 atgtccatgg gaaatcttgg taaaatttct cgtctggctg gcggaacgac gggttccgtt
     9901 atgacatttg gttccttaag cagcgattct acttctgctc caggacaatt accggtcaaa
     9961 gcattgaagc aaattattca attattaaat gaaccgttgt agaacaagtt tgaggtgata
    10021 ttttttggca aagaaaaaaa tagtaaaaac gaatgcaatc cgtatggtcg aacaaaaaaa
    10081 gattccctat acagagcacg aatatgagtg ggacgaaagc cacctaagtg cgtcatcggt
    10141 cgctgaacag cttccagaaa gccaatctcg tatatttaaa acactggttg ctgtgggcaa
    10201 tgtgacgggg cctcttgttg ctgtgatacc gggagaagcc gaattgaatc taaaaaaatt
    10261 agcaaaagtt tcagggaata aaaaagtaga aatgctccat ttaaaagatt tggaagcaac
    10321 gaccggatac atacgtggtg gctgctcgcc tataggtatg aaaaaactat ttcctactta
    10381 tttagatcaa attgcagaaa gctatgaaca aatcattgtc tcggctggca gaagagggct
    10441 acagatggag ttagcacctc aagatatctg tgcattaacg agcggacaat ttgcagatat
    10501 aaaacaataa atcatacgta taaaagaaag aaggaggccg ggatgttgtt tgtccagtct
    10561 ccttctttct tttttataca ttatgctctt tgcccggcat tcattcctgc tacatgccct
    10621 gtagcgaaag ccgctgtgat attaaagccg ccagtataac cattgacatc cagtaattct
    10681 cccgcaaaat acagaccatc aatgatttta ctctccaatg ttttaggata aatttccttt
    10741 agagaaaccc ctccgccagt tacaaatgat ttctcaatag gaaaagtttt gttgattgat
    10801 agcttaaact ctttgcatag ttgaacaaaa tcttgaattt gtttttgaga ggcttgttgt
    10861 cctgtcagat gatccatttc aagccgctca agaaagaaaa ctaacaatcg ctcaggcaat
    10921 ataccatgcc aagcattgac caagttcttt ttggttgctt tacttttttc tgtcaatata
    10981 tgaataagct cttgtttggt ttgtgtaggg aaacaatcca gagaaagtgt cactggttct
    11041 cccgttttct ctaattcttt attgacaaaa cttgaacaac gcagtgcagc cggaccagaa
    11101 ataccgaaat gggtgaaaag taaatccatc gtatgttcag tcaatacacg acctttttta
    11161 tttaaaacgc gtaaagtgat gtcttgtaaa gaaagccctt gaagtgtttt ctcctgaata
    11221 tacggttcat ccgatatcaa gggggactct gtcgcataaa gaggcgtcac cgtgtgaccc
    11281 actcgcttca cgatcttgta cccatctcca gtagaaccag tagatggata agtccgtcca
    11341 ccagttgtta agataatggc aggagcatgg aactcttcga attccgtgcg tataccgtaa
    11401 attttatgtt ctttgtggat caatttctct actcgagtag agaatagaag cgtcacatct
    11461 agttctttca gacgattgag caatgcttcg ataatcgttt ttgatttatt tgttacagga
    11521 aacatccgtc catgatcctc ttcttttaaa tgtacgcctt gtgattcaaa gaagttcatg
    11581 atatcgaaat tattccattg cgcaaacgta ctatataaaa actttccatt tccaggaata
    11641 tgtgcaatca aatcatcaac aggacgattg ttcgtaacat tgcaccgacc accacctgtc
    11701 atcagtagtt tttttcctgc ttttttgttt ttttcgatca agagtacgcg tgcgccttgt
    11761 tcagctgcac tgatcgctgc catcatcccg cttgttccag cgccgactac gatcacatca
    11821 tacagttttt ccattccctc acctcaagga aagtgtagca tatctgaaga aagaaaacag
    11881 aaaaaaaatg ttttgagcca aaagagaaaa tgagctaaaa tgagggaaga taagcaaagg
    11941 agcgtggaac gatgacaaac gcaacagaat atgttcaagc aattcaagaa aagctacatc
    12001 aaaaggatca agggcaagaa gaatttttac aagcaatcga tgaattcatg cccacggtta
    12061 tgtctttttt ggacactcat cccgaatata tcgaaaagaa tatcttagaa cttttaacgg
    12121 aaccagaaag agttattcaa tttcgagtgc cttggcaaga tgatgaagga aactggcgag
    12181 taaatagagg gtaccgtatc caatacaact cagctatcgg cccttataaa ggtggattgc
    12241 gcttccatcc tagtgtcaat ctaagtatct tgaaattttt ggcttttgaa cagattttca
    12301 agaatagttt aaccggtctg ccaatcggtg gaggaaaagg cggaagtgac ttcgatccta
    12361 aaggaaaatc tgataatgag atcatgcgtt tttgtcaaag tttcatgctg gaattgtcaa
    12421 aacatatcgg cccatcgatt gatgttcctg cgggagatat cggtgtagga gctagagaaa
    12481 tcggctattt gttcggcgaa tataaacgat tgaaaaaata tgatactggt gtgttgacag
    12541 gtaaaccttt ggatttttgg ggaagtaaga tacgtacgga agctactggt tatggattag
    12601 tgtattatgt caaacatcta ttgaacgaag aaaaagattc atttaatcaa aaaacagttt
    12661 ttgtttccgg aagcgggaat gtagccattt atgcaatcga aaaagtgcaa gagctaggcg
    12721 ggaaagttgt tacatgttca gattctagcg ggtacatcta tgatccagaa ggaatagatg
    12781 tgaaactatt gaaagaagtc aaagaaatca atagacaacg tttgacggaa tacgcaaacc
    12841 aacgtccttc tgctatttat catgctggag aatctgtttg gacattgaaa gaaaaagcgg
    12901 atatcgcttt gccatgtgct actcaaaatg aaatagacga aagcttggcc gaaatcttag
    12961 tagaaaatgg gatcaaaatc gtagcagaag gcgctaatat gccatgtaca gtaaaagctg
    13021 tttctgttct tcatgaagca ggcgtgtggt attgtccagg aaaagctgct aatgctggag
    13081 gagtagcagt atctgcttta gaaatgagtc agaacgtcca gcgattgact tgggaaagag
    13141 agcaagtgga tgagcagctg gatcaaatca tggaaacgat ttaccatacg tgtcgccaaa
    13201 cagcagccga gtacggcgac gaaaaaaatc tgcttcttgg agcaaatgtt gctggatttg
    13261 aaaaagttgc caaagcaatg gcagcagaag gtttagtttg aaaaatgaaa ctttcttgga
    13321 aatattgtca gcattttgtt tatttgcttg aaaaataata aaaaaaaacg taaagtatta
    13381 cctgtagagt aaacctataa ggaggagaac aaatggcaca cattcatttt gactattcaa
    13441 aagtagcacc gtttgtcaac gaacaagagt tagggtacat gcaaagccaa gtaacagcag
    13501 cccacaacga attacgggaa ggtactggcg caggaagcga cttccgcggc tggatcgatt
    13561 taccaacgaa ctatgacaaa gaagaatttg cacgtatcaa agaagcagct aaaaaaatcc
    13621 aatctgattc tgaagtatta gttgttatcg gtatcggtgg ttcttattta ggcgctcgtg
    13681 cagcgatcga cttcttgaat cacacattct tcaatttatt agatagcaac acaagaaaag
    13741 caccacaaat cttctttgcc ggaaattcaa tcagctcaac atatattgct gacttgatcg
    13801 aagtaatcgg tgatcgcgac ttttcagtaa acgttatttc aaaatctgga acaactactg
    13861 aaccagcgat tgcgttccgt gtattcaaag agcttcttgt taagaaatat ggacaagaag
    13921 aagcaaacaa acgtatctac gctacaacag ataaagcaaa aggtgcagtt aaagtagaag
    13981 cagatgcaga aggttgggaa actttcgtga ttcctgatga catcggcgga cgtttctctg
    14041 tattaactgc tgttggtttg ttaccaatcg ctgttagtgg tgcagatatc gatgcattga
    14101 tgcaaggtgc cgctgatgca agcaaagcat actcaagcga caaactagaa gaaaacgaag
    14161 cttaccaata cgcagcaatg cgcaacattc tttaccgtaa aggcaaagta actgagttgt
    14221 tgatcaacta tgaaccagga atgcaatact tctcagaatg gtggaaacaa ttgtatggtg
    14281 aatctgaagg aaaagaccaa aaaggcatct atccttcaag cgcaaacttc tcaactgatt
    14341 tgcactcttt aggacaattc atccaagaag gacgtcgcaa tatttttgaa acagttgtga
    14401 aggttgaaaa acctcgtaaa tcaatcacaa ttcctgaaca agcagaagat ttagacggtc
    14461 taggctactt gcaaggaaaa gaagtggact tcgtaaatac aaaagcattt gaaggaacat
    14521 tgcttgctca tacagacggt gacgtgccta acttgctagt caaaattcca gaaatggatg
    14581 cttatacatt gggttataca atgtacttct ttgaaattgc tgtaggtatt tcaggttact
    14641 tgaatggaat caacccattc gaccaaccag gtgtagaagc ttacaagaaa aacatgttcg
    14701 cacttctagg caaaccaggc tttgaagatc ttgctaaaga attgaacgaa cgcctgtaag
    14761 ataagggttt ttcgctagat ttaaataatc agtgatagaa acatgttcgc tttaaaatga
    14821 aaactggtac ccatttaggt actcaaacat actgcatcgt ttattcagaa taaggggctg
    14881 tgacaagacg tttgtcacag tcccttgttt gtatagaaat cgattgattg ccttgctctt
    14941 tagtttgttt taggacttta agaacagtga ggctgacttc gaaggttaat tctgtttttt
    15001 tgttttctag aatcagtaag caaaaaagca ctcacttcga gtgctttttt gcgatatatt
    15061 cttagcttaa gataggccta atttttcttt tacagatgag ggtaccttat cgctggtaat
    15121 tacttttttc tctatgatcc gcttttcgct tgctttcaac tgcaagatcg ttccttcggg
    15181 gtaagcagaa ccattggcag agtcaccaga agtaatgaca tcgaaatcta actgtctttt
    15241 ttcaccgttt tcagcataag catctacgtt gtatttaaaa cctcttccaa ttacttcccc
    15301 attatctgct ttgatatcag tttctgcagg cattggagct ttgactaccg cataataatc
    15361 tttacctaca taagtagact gatagtagtc ccaacctttc catcctataa aagcaatcaa
    15421 tatgataacg atcaacccta aaattttttt catgaaagat ctcctttttt atttttatta
//
LOCUS       DAZKBH010000064        14815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000064 DAZKBH010000000
VERSION     DAZKBH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.064"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG8OO_001851"
     CDS             complement(296..1024)
                     /locus_tag="ACG8OO_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045723.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG8OO_001852"
     CDS             complement(1002..2576)
                     /locus_tag="ACG8OO_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9045724.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG8OO_001853"
     CDS             2805..3743
                     /locus_tag="ACG8OO_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9045725.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG8OO_001854"
     CDS             3755..4666
                     /locus_tag="ACG8OO_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9045726.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG8OO_001855"
     CDS             4690..6162
                     /locus_tag="ACG8OO_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9045727.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG8OO_001856"
     CDS             6213..6824
                     /locus_tag="ACG8OO_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9045728.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG8OO_001857"
     CDS             6854..8767
                     /locus_tag="ACG8OO_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFP9045729.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG8OO_001858"
     CDS             8895..11876
                     /locus_tag="ACG8OO_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFP9045730.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG8OO_001859"
     CDS             11873..13534
                     /locus_tag="ACG8OO_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFP9045731.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG8OO_001860"
     CDS             13574..14506
                     /locus_tag="ACG8OO_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFP9045732.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAZKBH010000065        14526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000065 DAZKBH010000000
VERSION     DAZKBH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.065"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(330..692)
                     /locus_tag="ACG8OO_001861"
     CDS             complement(330..692)
                     /locus_tag="ACG8OO_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045733.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(766..1146)
                     /locus_tag="ACG8OO_001862"
     CDS             complement(766..1146)
                     /locus_tag="ACG8OO_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFP9045734.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1228..2403
                     /locus_tag="ACG8OO_001863"
     CDS             1228..2403
                     /locus_tag="ACG8OO_001863"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP9045735.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2480..3064)
                     /locus_tag="ACG8OO_001864"
     CDS             complement(2480..3064)
                     /locus_tag="ACG8OO_001864"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9045736.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3265..4263
                     /locus_tag="ACG8OO_001865"
     CDS             3265..4263
                     /locus_tag="ACG8OO_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9045737.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4375..4884
                     /locus_tag="ACG8OO_001866"
     CDS             4375..4884
                     /locus_tag="ACG8OO_001866"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9045738.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4914..5573)
                     /locus_tag="ACG8OO_001867"
     CDS             complement(4914..5573)
                     /locus_tag="ACG8OO_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFP9045739.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5575..6339)
                     /locus_tag="ACG8OO_001868"
     CDS             complement(5575..6339)
                     /locus_tag="ACG8OO_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFP9045740.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6347..7024)
                     /locus_tag="ACG8OO_001869"
     CDS             complement(6347..7024)
                     /locus_tag="ACG8OO_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFP9045741.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7061..8446)
                     /locus_tag="ACG8OO_001870"
     CDS             complement(7061..8446)
                     /locus_tag="ACG8OO_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFP9045742.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8656..8740)
                     /gene="ffs"
                     /locus_tag="ACG8OO_001871"
     ncRNA           complement(8656..8740)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8OO_001871"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="ACG8OO_001872"
     CDS             complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="ACG8OO_001872"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9045743.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9385..10242
                     /locus_tag="ACG8OO_001873"
     CDS             9385..10242
                     /locus_tag="ACG8OO_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045744.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10323..10658)
                     /locus_tag="ACG8OO_001874"
     CDS             complement(10323..10658)
                     /locus_tag="ACG8OO_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045745.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10725..12095)
                     /locus_tag="ACG8OO_001875"
     CDS             complement(10725..12095)
                     /locus_tag="ACG8OO_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFP9045746.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12173..12709)
                     /locus_tag="ACG8OO_001876"
     CDS             complement(12173..12709)
                     /locus_tag="ACG8OO_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9045747.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13018..14499
                     /locus_tag="ACG8OO_001877"
     CDS             13018..14499
                     /locus_tag="ACG8OO_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9045748.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4475 a         2892 c         2358 g         4801 t
ORIGIN      
        1 actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta aattttttag
       61 aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg tatttcttcc
      121 ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat
      181 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
      241 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      301 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      361 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      421 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      481 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt
      541 aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat
      601 tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac
      661 ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat
      721 ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc
      781 tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt
      841 ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc
      901 tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat
      961 gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt
     1021 ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac
     1081 gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc caattttata
     1141 agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa
     1201 gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa
     1261 gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt
     1321 cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt
     1381 gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct
     1441 atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt
     1501 acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat
     1561 tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa
     1621 cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct
     1681 gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac
     1741 aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta
     1801 aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat
     1861 acatttactt ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact
     1921 tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat
     1981 ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac
     2041 acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa
     2101 ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt
     2161 ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc
     2221 tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta
     2281 aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt
     2341 ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat
     2401 taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat
     2461 tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta
     2521 caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt
     2581 ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat
     2641 acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg
     2701 gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg
     2761 ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt
     2821 cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga
     2881 aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa
     2941 tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac
     3001 ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg
     3061 ccatattgtt tcctcctaat atttgcatac ttcataataa taataacata atacctaacc
     3121 tctcgtaaaa atactagatt tgttttcttt tatttttcat cactagatac attctataac
     3181 ttaccgtttc gatttcgaat tcaaacattt ttgagaagca ttctcaatta aattctgcta
     3241 tagtgtgaaa agggaggaat ttttatggaa atctatgaca ttacaatcat cggtgcaggt
     3301 cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt
     3361 gacagtctac ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa atatatttac
     3421 gatattcctg ggtatccagc aataaaagca agtgaattaa tcgatcaatt agaaaaacaa
     3481 ttaactactt ttaaccatac attccattta aaagaagaag ttctctcgct tactcgtgaa
     3541 gacgaagtta tcgagattac tacaaataaa ggcatccatt attccaaagc agtcatttta
     3601 gctttaggaa atggttcatt ccagccaaga aaattgaatt tagataatgc tgaatcattt
     3661 gaaaaccacg gattggatta tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc
     3721 gctattgctg gaggtggtga ttctgctatc gattggacgt tgatgttaga gcctattgca
     3781 agcgaagttt acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca
     3841 cgcttgaaat cttcatctgt caacttgctt actccctatc tcatcgatgg attatcaggc
     3901 aataatgggg aactgacaga tatccggtta aaaaaagtga aaagtgacga gacgattgat
     3961 ttgatgattg attctctgat cgtcaattat ggattctctt ctaatttaga acatctttct
     4021 tcttggggac ttgatagtac cagaaatgcc attactgtaa aatcagatat gtctacttct
     4081 attccaggcg tttatgcagc tggtgacatc tgtacctatg aaggaaaagt gaaactgatt
     4141 gctactgggc tcggtgaagc acctactgca gtgaacaacg ctttacacta tattaatcca
     4201 aaggaacgaa ctcagcctgg tcacagcaca agcttatacg ataaaaatct tcgtcctagt
     4261 tgatttttta tatctctttg catttcaagt taaacccgaa agcagtttct tgcatttttt
     4321 tcatgttccg ttaacatgaa atagagaaag aaaaaataaa gggggagcaa taatatggtc
     4381 gtcaaaactg agactttaga agaaaaggca agagaactat tgaaggaacg tggcgtaact
     4441 gtagaagata tcgcacaact tgtcatgttt ttacaaaaag actatatttc tgatttgact
     4501 ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc
     4561 attatcactg gaatccaatt agatatatta gcagaaaaga aaaaattgat gagtccgttg
     4621 caacatatta tcgaagatga cgaaggcctt tacggtatcg atgagatcct agccttgtca
     4681 atcgtgaatg tttacggtac gatcggcttt acgaattatg gttatatcga caaggtgaaa
     4741 cctggtattt taaaagaact aaacagccac gatggcgttc atgtccatac cttcttagat
     4801 gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat tagcacacaa agaaccagaa
     4861 aaaagaagtc gccttactca ataattcaac aaaaaaagaa agaaaacgtc tatttagacg
     4921 agttctttct tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa
     4981 aataattcta ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt
     5041 acattgatga ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt
     5101 tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact
     5161 ggcagtaaca tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc
     5221 aaatatttga taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact
     5281 aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct
     5341 ggcaagctga ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt
     5401 ttcagtaacg tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca
     5461 aatacgtata gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga
     5521 aataggctga acaatccagc atattccatc cactgccatc tcgaattttt catattagaa
     5581 gctccattca tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc
     5641 ttcttctttt gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc
     5701 ttggatatct gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa
     5761 tacttcaacg gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt
     5821 ttgggttgct gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc
     5881 agtaggtata ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag
     5941 tgttgcttta actacttttt cataggtcag ataattatct aagcctacca ctacatagtc
     6001 aggagcctct tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc
     6061 tccgataaca tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa
     6121 agtagctgtg tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt
     6181 agctactgtt tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct
     6241 tttttgcaat tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat
     6301 cgttccgtct aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt
     6361 ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta
     6421 ctttttcaga ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat
     6481 tagatgcgac tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt
     6541 ttcggcgctg agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac
     6601 aaccaaaatt gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat
     6661 gcgcccgtcg attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat
     6721 ctcctacgat ataatcatat cgggctagta catcactgta acgttctcca agcttttctg
     6781 cattaaatcc ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat
     6841 tactgtcatc taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa
     6901 cgatttcttc caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg
     6961 attttttctt agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag
     7021 acatgcgttc accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag
     7081 ctttcaagta atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc
     7141 cagcaatctc aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag
     7201 tataattttt attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat
     7261 catatgtgat tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct
     7321 tcataggata gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca
     7381 gatcatttcc acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa
     7441 gctgatcctg attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata
     7501 ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat
     7561 gctcattgaa gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta
     7621 ataattcatg cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg
     7681 tcattgtttc ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga
     7741 tggttaaatg gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat
     7801 cattgattac gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa
     7861 ttgctggtat ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa
     7921 ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat
     7981 tccgtatgtc ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta
     8041 gtccgatttt cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg
     8101 gctggagcgt atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt
     8161 ataaatgatt caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg
     8221 catcataacc aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca
     8281 atggatgcca acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt
     8341 tcttttcgtt ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta
     8401 aatgagaatg cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct
     8461 ctctttatct ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca
     8521 gagtttttcc tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct
     8581 aaaaaatcag gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag
     8641 tctgttgtcg tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc
     8701 cagacctgac acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata
     8761 ccttattttt cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt
     8821 cggaaaaagt cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat
     8881 gcttggtggt taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct
     8941 tttggatcat acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg
     9001 cacataggac aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc
     9061 actttttcac aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg
     9121 gtttctctaa ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca
     9181 atcggaactt catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat
     9241 ttttcttttt ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt
     9301 tttcttgttt tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta
     9361 aaataaatac aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa
     9421 gcacagaaaa aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat
     9481 gcttttatct ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg
     9541 ttattccctg taatcaacga tcaaaaaaaa cacccatggt atcgctatct ttttaaagat
     9601 gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga
     9661 gaattcttaa aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat
     9721 ttcttttttt atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt
     9781 gatgctactg aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg
     9841 tctgtattcg ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa
     9901 gaaagaaaaa tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct
     9961 atgcaagatg tggctttaca ttactataca aaagatactg taaaagatag taagttgatc
    10021 aaaagttaca tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg
    10081 aatgaattag gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta
    10141 aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat
    10201 ttactgtttt gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt
    10261 gtgacaataa ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc
    10321 tcttacatca ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct
    10381 ttgattgaat caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt
    10441 cccatcaaag aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca
    10501 tttccatcaa atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg
    10561 atcttttcca ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct
    10621 acgcttaatc cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct
    10681 atctccatca tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat
    10741 cctttaggga aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc
    10801 ttatctaata ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct
    10861 gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt
    10921 gtagcaagcg ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc
    10981 aaacctgtac gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa
    11041 agatggtcgt tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac
    11101 tccgtccaga gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc
    11161 ttttctttat gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca
    11221 ccctgatctt tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat
    11281 tcagaacgtc ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa
    11341 ttttctacaa gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa
    11401 taaatcagtt gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga
    11461 cgtttttgac agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt
    11521 cggaacattc cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa
    11581 aaatgaggga caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat
    11641 cgttctatat tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat
    11701 aaccctttcc ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac
    11761 aaatccaaaa ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt
    11821 ggttcttggc tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca
    11881 agaaatccct cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc
    11941 atgtccaatc ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt
    12001 tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata
    12061 aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac
    12121 aatctttttt acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg
    12181 actgttcctt taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag
    12241 cgttcatcat ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat
    12301 taagccatac caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg
    12361 agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac
    12421 gaatactttc atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat
    12481 cggaactgca ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct
    12541 cttttaaccc gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga
    12601 actctgctaa ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac
    12661 ttttgaacag caaatattct ttactataat ttgttactct ccccatcatt cttacctccg
    12721 tctcttttca tctacccata aataaataac ataaataatt ctaaaaatat tattgataat
    12781 tatcaaaaca tatttagtta tatccattaa aaatcaattt ttccgcctta aaaacaagaa
    12841 tatcttgaca aaatacccta ggaaataaac aatagaataa acaaggtaac aaaaaaatta
    12901 aataacgtta tataaaaaag aaggcgacac tctgtgagaa taacacagac tgaaatgact
    12961 ttccactcac agtttttgtc taactatata caaaaacaga ataaaaagga ggtatttatg
    13021 ctaattgaag caagaaaaaa agagttactt gttctacaaa tggtcattca aaaaaataat
    13081 tgttctttac aagaattatc caatgactta catattccaa aacggacaat caaagaactg
    13141 atccgaaaaa taaatgtatc actcaagcaa tttttattga ttgatgaatt tatttattct
    13201 aatcataaag gagagatcca aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt
    13261 tctaagttaa aattatgcta cctaaaagaa tccaatcgtt ttaactattt gatgttacta
    13321 atcaattatc cacaaactgc tgtaccaaaa aaatatttac ttgaacaact ttatatatcg
    13381 ccctcctatc tcgaaaaact gacaaaacag ctcaatcatt ccttaaaaaa attccattta
    13441 cggatcctat ctgtaaaaaa ttgctgtact cttgaaggaa atgagttatc tatccgtctc
    13501 tatctttact tttttctttc agatacattt ggaggtaacg attggccatt ttcctcactt
    13561 gaattaaatg cactaaaaat gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa
    13621 gaaaaaaata tctacttgct gttaacttta ttcatattac gaaacaaaca acaggcatat
    13681 tttccaaaaa cttcttctga aatcactgaa ttgatggact tattgcagca tacacatgat
    13741 tattcagaat actttacttc tttttcagtg agtcgccctt gtgaagagac agaaacagag
    13801 agagcctact ttaattattt tttctattgt ttttttccaa atcaaatctc ttttcgacaa
    13861 aaacaggaga ttggtttttt attttccaca agcaaaaacc ttatttgtcg acaagtcggt
    13921 caattgatga cttacttaac tcaacagttt cctctcaacc atttagaaaa taaaatacca
    13981 atttattttt attttcttgt gttggcagtt gtttttttcg aactgttggg tgaatccagc
    14041 agtagttttt ctacactttg ctttccagaa gccacgtcca atcattctag ttcttttata
    14101 caggaaaaga aattaagaga aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac
    14161 caaatcagtc aataccttag tcaattgatc caacacttac tacaaacaga aacgaaagag
    14221 cagttactca tccatttgca gataaccaaa cagtttacag gaaattatca tcttcaaagc
    14281 catttgcagc agttatttcg caaagatgct gttaaaatta ccgcatattc agaacatgct
    14341 gatttgatca ttacagatca gcatgagaaa atttcagatg aaacggagac tttctatctg
    14401 gatacaacgc acaatacagt gttatttggc aagttaataa aagcggttca tgaactgtat
    14461 atgaaaaaag gttatgagaa caaaacaaag aggatgtgac aaaattcttg tcacatcctc
    14521 ttattc
//
LOCUS       DAZKBH010000066        14457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000066 DAZKBH010000000
VERSION     DAZKBH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.066"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(103..999)
                     /locus_tag="ACG8OO_001878"
     CDS             complement(103..999)
                     /locus_tag="ACG8OO_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9045749.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(992..1672)
                     /locus_tag="ACG8OO_001879"
     CDS             complement(992..1672)
                     /locus_tag="ACG8OO_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9045750.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1673..2455)
                     /locus_tag="ACG8OO_001880"
     CDS             complement(1673..2455)
                     /locus_tag="ACG8OO_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9045751.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2448..3365)
                     /locus_tag="ACG8OO_001881"
     CDS             complement(2448..3365)
                     /locus_tag="ACG8OO_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9045752.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3529..3783)
                     /locus_tag="ACG8OO_001882"
     CDS             complement(3529..3783)
                     /locus_tag="ACG8OO_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045753.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            4640..7867
                     /gene="fss3"
                     /locus_tag="ACG8OO_001883"
     CDS             4640..7867
                     /gene="fss3"
                     /locus_tag="ACG8OO_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFP9045754.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7980..8294
                     /locus_tag="ACG8OO_001884"
     CDS             7980..8294
                     /locus_tag="ACG8OO_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9045755.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8307..8681
                     /locus_tag="ACG8OO_001885"
     CDS             8307..8681
                     /locus_tag="ACG8OO_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9045756.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8691..9026
                     /locus_tag="ACG8OO_001886"
     CDS             8691..9026
                     /locus_tag="ACG8OO_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFP9045757.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9109..10449
                     /locus_tag="ACG8OO_001887"
     CDS             9109..10449
                     /locus_tag="ACG8OO_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9045758.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10527..11201
                     /locus_tag="ACG8OO_001888"
     CDS             10527..11201
                     /locus_tag="ACG8OO_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP9045759.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11194..11358
                     /locus_tag="ACG8OO_001889"
     CDS             11194..11358
                     /locus_tag="ACG8OO_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045760.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11434..12576
                     /gene="dcm"
                     /locus_tag="ACG8OO_001890"
                     /pseudo
     CDS             11434..12576
                     /gene="dcm"
                     /locus_tag="ACG8OO_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12566..12856
                     /locus_tag="ACG8OO_001891"
     CDS             12566..12856
                     /locus_tag="ACG8OO_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045761.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13115..14299
                     /gene="mobT"
                     /locus_tag="ACG8OO_001892"
     CDS             13115..14299
                     /gene="mobT"
                     /locus_tag="ACG8OO_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9045762.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14296..14433
                     /locus_tag="ACG8OO_001893"
     CDS             14296..14433
                     /locus_tag="ACG8OO_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFP9045763.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         4626 a         2643 c         3024 g         4164 t
ORIGIN      
        1 atcaataaaa aagcctgcaa tatttatatc acaggctttt ttctctatta aatatatata
       61 agataataca tttttctact agttctcact aatcttgtat aattaaaccc tccgcaactt
      121 gacggagatg gagaagatgc ccccgtctaa tctagcggtc atctctcctc ccattccttc
      181 caccaatttt tttgacaggt aaagcccgat tccgctagat tccgtatttg tgcggctggt
      241 attttcggta taaaaacgct ggaagacttt ttcaacagag gtttgcgttc ctggtttgat
      301 aggattggcc actgttaatc gaatgaatgg atcctctttt gaaaatgtca cctttgcttg
      361 tccatctgca taacgcaata aattctgtat gatattttgc agcactcgca gcaacatgag
      421 cggatctgct aatacagttg tatttatcga gtgttcagcg aacgctacct cgatattttt
      481 ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc acctctttag aaaaagaaag
      541 cgtatggaga tccatttctt tttgcttcgt ctctattcga gcaagatcat aaaactgatc
      601 cgtcatactg agtaatcttg caacagattg tttgatgatc gttaaatact tctcccgttt
      661 ctccgtatcc tctacctgtt ccagtaattg tacataaccg ttaatagcag tcaaaggagt
      721 tctgagatca tgagaaaggc cgacgatcga tagtctgatg ttttcttcca tttcttgaaa
      781 tgcttgtcct ttttgttcat tttcatccaa ccatgtattc agtgcatcta ctaggtccat
      841 tagattcttt tctcgaaatt cgactgaaag acgaccacca tattcttgtc gttcagaaag
      901 agtggcgatt ttcttagtta atctcttgat ttccctttgc aggaagaaaa taaaacttcc
      961 cataaagata acaacgagaa gcaacagcca tttaatcaaa tctaaacccc actccccaca
     1021 ccgtatgaat atacacagca tgtgcctttg ctagcttccc tcgaagattg gaaatatgca
     1081 tattcactgt tttctcacta tcaaaatatg gttcttgcca aactgtttca taaagatttg
     1141 ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt cagcagatcg aattctcttc
     1201 ctgtcaaatg gacagccaca ccattcacat ttacatccct cgactctttg tccagtaaga
     1261 tttccttgta atagatgcgt tgcgacagat cttttacttg gacagaagtc tgatgacgaa
     1321 gttggatgcc gattcgtaac aaaagttctt cgatatcaaa tggtttcgtc acatagtcat
     1381 ccgctcctgc tgccaacaag gaaactttat ctctttgatc gctttttgct gaaagaacga
     1441 tgatcggaac attcgatgtc tgtcggattt ctgctaacaa tgcttcacct gaaagccctg
     1501 gaagcatgag atctagaagg atcaaatcga caggatatgt gcttaacaat aattttcctt
     1561 ccgtacctga ataagcatca agaagtttgt atctctgttc aagaacctcc tccagtaagg
     1621 aatgaacagc ttcatcgtct tcgataatca aaatagtttc cagcaggacc acctacttaa
     1681 tgtcagattt ttggaaaata aacattccta gagctgatga taccacgatt gttactagac
     1741 tgaccagaat cattttcggt gcttgcgaat aagttgcttg ttgcgcaaag tccgtataca
     1801 tgagccacgt ttccgtaaac cactccgtta gaaattccaa gcctttgatt tttgcgaaaa
     1861 caccgggaat gaaggccgtt cccatgatat aaagaaaaga aagaagcaaa ctgacactgg
     1921 tacttctagt gatgaatgtc aaagccactg gaacagataa tgtccctaat gtcagcagaa
     1981 gcaatacagg aaatactttt agaacataat ttccatagtt tttgattccc tcaacttcac
     2041 caaatccgaa agcaagtgtg taagcaagca tggctagtcc cgtgatcaaa accacgccaa
     2101 gtgttgtgat caaaagcgta acgagaagtt tcgacacata ataagtcatt cgattccgtc
     2161 ctaaagataa cgtattgcga atcgtaccgt tttgaaattc ttccccaata taaaagcacg
     2221 caaatgctgc gaaagccaga agaatccatg cagccatttt ttctaccatt aaaatccctg
     2281 ttgcaccgtt gatgactgta tccgttgctt ggtttaattc attgccgtat tttgtatgca
     2341 tataccattc gttccaagaa taaaaaagcg gaagtacgac catcagtcct agtgagaccc
     2401 acattttacg gctcttccat acttttattt tttcagcagt taatagatta aacatgtgga
     2461 ttgcctccag ttaattgtaa gaaataatct tccagttttt gcccaactag gtgaatacca
     2521 tccacaggaa cttttgcttc taccaatgct tgattgatgg ctgctacttg gtcaattcct
     2581 tcaaaaagct ggataacacg gctagattgt acaaaataat ccctataacc aagagagtcg
     2641 agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt ttataaattg acgagattct
     2701 tgtgccaatt cttctttcga taattctttg atcaagtgtc cttcatgcaa aataccatag
     2761 tgagtggcga tctgtgacaa ttcatctagc aagtggctag aaagcaagac ggtgatgccc
     2821 cgttctttta ccaaacgttg gatgatttct cgcatttctg ccatccctgc tggatccatc
     2881 ccattcgtcg gttcatccaa aatcaaaaat tctggattcg tgatcagtgc atttgcaata
     2941 gctaaacgct ggcgcatacc taaagagaaa ttctttgctt tctttttccc tgtattttcc
     3001 aatcgcatca ttttcagcag gtcttcgatt tctctatccg acacaccgcc gttcgctgct
     3061 tggatacgta ggttatctct tgcagttaat tctggataaa gagcaggggt ttcaatcgat
     3121 tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc caaataattc gatcgaacct
     3181 tcatcaatcg agatcaaacc cataatagtt ctcataaagg tagacttacc cgctccattt
     3241 tccccaatca atccatagat cattccacgt ttgatttcga ttgatacttt atctaatgct
     3301 ttggcagcac cgtatttttt ggttatacca gttgctttca agaccgtttc atttccaaaa
     3361 tccatactta tccttctttc ttattgatga gtgaagtata gttgacgggt ataaagaaag
     3421 cagccagaaa tgtataaaga aagtgtaaat tttccaattg aatgtcaaaa aaaacaatcg
     3481 gacaatacgt gtctttccta ttatcaggca gtttgttata cgcttgtatc agtttcctga
     3541 ttctttcttt tcgaccaaag tcgtttctct gtattcctct tcactgaaaa tttttgcttc
     3601 tttcagatca agcttgagta attttaccag accggttata aggataacta tagcaagact
     3661 ccctcccaga aaaattagac tgtctcgcag acgtggttgc gttgtcttcc aatcaaagag
     3721 cgcaccaaac agtgctaatc caagtggact ggccaccatg gtaatcgttg ttatcgttga
     3781 cataagtcgt ccttgtatat gcgttggaac ttctcgctga agccagacca tataaaaagc
     3841 aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa taatacatgc cgtttgggat
     3901 ttacaattag ctaccaaata agaggttatg acaaaagact tttcacaatc ccttcttccg
     3961 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatattta tcaaacatga
     4021 gaagcaattt ttgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
     4081 agaagcggat aaataatatt gtttgatgat actttatctt tttcccgttt gtttatagat
     4141 cgaatgatat tatagaccaa gaatcctacc tattaacata gctaaaaaca caaaaaaggc
     4201 actttccaaa ttgtggaaag tgccagaaaa gttaggatta aggtaactgc gctcagcatt
     4261 gcttcgccca gtacgattct cgtaactcgt gaactcgaac gataatcgca attaacgttt
     4321 tgagaactgt gaagctttac gggctttctt aagacctggt tttttcttcg tatcattgaa
     4381 taagtttgaa aaaccttgat ttataaaggt tttcccgtaa atatatttta ttatatttga
     4441 ttaaatttga aatgtttgtg ttctaaaatg tgttctaatt taaaggcagt atatttcgta
     4501 ttttagatta aatttttagg ctcattcatt ctcaaatcca ttctattcag tcaataaaaa
     4561 ttttaaaaag actttataaa caaagatgtt caagcgagca tcttattttt ttgtgcttaa
     4621 tttgaaagga gaacatttca tgtataaaaa acaaaaaaag aaattatgtg tattaactat
     4681 tttgagtttg gctgttcaag caattgttgg agcaattgct cctacgatta cttttgctga
     4741 tgaaattacc catccacaaa cggtaaccgt agaactagat ttagcacatc aatatgctgt
     4801 tgaaggaact tttagtgatg gtcgtccaat gtcagaagtc actgttccgc attatgcagt
     4861 gtacaacggc tttaaacaag atattttctg tattgaacca ggagtcccga tttacaatga
     4921 gttcactcct ggctatgaga aaaacccgtt accagatatg tccgataaag ccaaattagt
     4981 ttccgtttta tggaaaaaag taggtacaga tgcagatact catattgtgg cacagaagat
     5041 gatctggcaa gaagttaatg ggtataccct tcactcaata aaacgatcag atggtagtgc
     5101 cgtaaatatc gcagccattg aggcaaaaat aaatcaagcc attgacgatt atcagaagaa
     5161 accaaccttc cataatagta cggctaaaac agtattaggg caatcgacta ctgtgacgga
     5221 tacgaataat ctgaatttgt cagagttcga tgaggttgtg gaaaatgccg caaacattga
     5281 ttatcgtgtg aatggtaatc agttgataat caccccaaat gccaattcaa aagaaagtgg
     5341 tgtgctgacg cttaagaaat ctgctggtac cggaacacct gtcgcctata aaaaggctgg
     5401 ccaacagact ttaatggccg gggcgattga taaacctaat acctacacaa tcaaaattga
     5461 tgtggaaacg gagggttctt taaagatcaa aaaagtcgat aaagaatcgg gtgatgttgt
     5521 accaggaacg gttttccatt tagactttgg aaaaacttta cctgcaaaag acgtgactac
     5581 tgacaaagaa ggcattgcta cattggatga gattcctcat ggtacaaaag taaccattac
     5641 cgaaaaatca gtaccggcgc cttatacgat tgataccacc cccatgacta ccaccattaa
     5701 agcaggcgaa accatttacg tgacttcaaa aaacgcacgg gaaaaagggc aaatcattct
     5761 ggataaaagc ggagttgaaa cagggagtga tctttggaat gacaattatt ccttagctgg
     5821 taatacgttt gccattcgca aagacagtcc aaccggtgaa atcgttcaag aaatgaccac
     5881 ggatgaaaac ggtcatgcgg aaacaccaaa agagattgct aatgcgttgg aattgggaac
     5941 ctattatgtg accgaaacta aagctagtca tgggttcgtg aatacgttca aacctgtaaa
     6001 agtcgaattg aagtatgcca atcaaaccgt agcccctgtt accagtaacg taaaagggca
     6061 aaatcaagaa gtcactggcg aaaccacttt gacgaaagaa gacaaagata ctggggataa
     6121 agcacaaggt aaggcagtct ttgagggtgc tgaatacacc ttgtttactg ctaaggatgg
     6181 aaaagctgtt aaatggagtg aggcttttaa accagagtta gcgaagggaa cgaaaggttc
     6241 tgatgaaacg gtcactttga ctttagacga aaagaaccaa gttgccgtta aacatttagc
     6301 tatcaacgag tattattggc aagaaacaaa agcgcccgaa ggatatagcc ttgatgaaac
     6361 aaagtatcct gtatccatca aaaaggtgga tgatagcgaa aaaaatgccg tgattacccg
     6421 aaatgttacg gcaaaagaac aagtgattcg ctttggcttt gatttcttta aatttgctgg
     6481 atcagctgcg ggcactaccg aaactggctt caatgatctg acctttaaag tgtcgccatt
     6541 ggaagggacc aatgaaatca caggtgccga agatgaagcg accacagctt ataacgagca
     6601 attagggttt gatggttatg gcaagtttga aaatcttcct tatggggatt atttacttga
     6661 agaagtagaa gccccagaag gttttcaaaa gattacgcca ttagaaatcc gttctacttt
     6721 taaggaaaac aaagaagact ttgtgaagag tgagtatgtc tttaccatta ccgaacaaga
     6781 ccaaaaacaa ccaattaaaa cggtgaccgt tccttacgag aagctgacga acaaagcatt
     6841 ttctgttagt ttgaaccgtt taatgctcta tgatttgcct gaggaagaag atagtttaac
     6901 ttctcttgcg acttggaaag acggaaacaa agaattaact tctctggatt ctaccgagct
     6961 agttgataaa ttgagttaca atctacatga aatcaaagaa gactggtatg tggtagcgca
     7021 agccattgat gtggatgcga caaaagctgc ccaagaaaaa gatgaaaaag ccaaaccagt
     7081 ggtgattgca gaaacaagcg caacgttggc gaacaaagag aaaacaggaa cttggaaaat
     7141 tcagcataaa ttaaccgccg aacaagtttt aaataaaact attgtcttat tcaactatgt
     7201 gtatgaaaat aaggaagctt ttgaagcagg cgataagcca gtagcgaagg atgttagctt
     7261 gaataaccaa gcccaaactg tcagttgtac agtggaacac catgtttcta tccaaacaaa
     7321 ggcccaccta gaaaacggtt ctcagacctt tactcatggt gatgtggtgg atatgtttga
     7381 tgatgtgtcg attactcatg atgtactgga tggctcaaaa gaagctttcg aaacaattct
     7441 gtatgcttta ttaccggatg gtacaaacaa agaaatttgg aaatctggca agattgatta
     7501 tgaagtaaat gataaagaat tcaccaaaac tgtgcttgcg aaaaaagtag ataccggaaa
     7561 atatccagaa ggaaccaagt ttacttttgc ggaaatcaat tacgataaag atgggaatat
     7621 caatggaaaa cacaatgaag atttgaaaga aaaatctcaa accttaacac caaaagaagt
     7681 tccaaccata ctaagtacgc cgaaacaacc ggaaacacca actgttccaa gtggctctca
     7741 agaatctagt cccacagtga agaccttccc tcaaaccggg gagaaaaatt ccaatgtttt
     7801 actgttcatt ggttttactt tgatttttgc gacggcgggc tattatttct ggaatcgccg
     7861 gaactaaggt gatgagatga tacaagtaaa tcctaagaac caacgtgcgc gaccgcctcc
     7921 taaaaaagta gtcgcgctat ttttgtatcc aaaactaaca atgaaaagag gaaaagaaga
     7981 tggaattgaa atttgtcgtg ccagatatgg ctgaaacctt tggaaagtta agttatgccg
     8041 gtgaaggaga aattataaca gaaggatatg gacggaatac tacggtgatt ggtcgcagtt
     8101 atcatttgta ttccagtaag caacgggccg atgatattga ggtggtagta gctgcggaag
     8161 ccggtgaaaa ggattttgat caagaccaac cgctaaaagc cgtgaatccc catttggttg
     8221 ccaaaggcta tgagattgag aatcgtgggt ttactgatta cgtgttgtat gttgatgatt
     8281 tagtgaaagc ataggaggaa aagaagatga gattaacaga aggtattgtc gtagattcag
     8341 gtttaacatt tgggaagttg cgtttttccg cattacgccg agaagtacgg aagcaaaatg
     8401 aggatggaac aattggtaat gaggtaaaag aacgaaccta taatttaaaa tcctctgcac
     8461 aagggcgaat gattcaagtt agtatcccag ctaatgtacc cttacgcgag tttgcttatg
     8521 atgcagaggt agagttggtc aatccaatcg tggataccgt tgccaattat gtttttcgag
     8581 aaggaactac cgttaattgg ttcattaagg cggatgattt agtcttgaag cggcagccga
     8641 atcaaggaaa tcctacgaac caaaatgaaa gaaagaaata ggtgaagatt atggaaatta
     8701 atgttgaaca tattttagat tgtctcgatc aatatggaaa aggcgagctg actgaggaac
     8761 aactcgttaa agcattaacc tctgatgaaa aaatgtttct gattatacat caaggtttac
     8821 ttggagaaaa tagcgacagt gaagaagact ctgatgtact agactggtta ggagaagaag
     8881 agtctttctt tatagttgtc gaagtaaatg aaaacttatg tcggcaggca gaatccattt
     8941 tggaagaaat tggtgtggag atgccggatg ctattgaagg ctttttaaat caattggttg
     9001 aaacgaaaca actcccctcc tcgtagcagt aaatgactag cataactttt tgaaattgcc
     9061 taatgaaatc ctattgggct ttttcgattg gaggtggaaa ccatcaaaat gtacaaagga
     9121 catcgcatac gcgcaggaga tcagcacttg gtttatcact ttgttcttgg ctggctactc
     9181 gctctgttca tcggttggat gagcgtcttt tattttcaag agtttagaca atttgatatt
     9241 tctaagctat cactttctac tattgaaatc gtttggtcta taaaagattt agtatgtctg
     9301 cttggaagtc tcgctttttc aggagctatg atattgcttt atattcactt ttttcttgat
     9361 cattggcgaa gcttgtggca tcgacaaaag ctagctcgga tgattctaga gaaccattgg
     9421 tatgaagtga aacaaactca aagtgaaggc tttttcaaag atttgaatag tagccgaacc
     9481 agagagacta tcagttactt ccccaaaatc tgttatcgaa tgaaagatgg attactgtct
     9541 attcgcgttc aaatttcact tgggaaatac caagatcagc ttttaaaatt ggaaaagaag
     9601 ttagaaagcg gactgtattg tgagttagta gaaaaggaac tcaaagactc ctatgtggag
     9661 tatactttac tgtatgatat gatcgccaat cgaattggga tagacgaagt agtggcagaa
     9721 aatgggactc tgcgattgat gaaaaatcaa gtatgggctt atgattcttt accccatatg
     9781 ttaatcgctg gtggcacggg tggagggaag acctatttcc tcctcaccat tatcgaagca
     9841 ctattgaagt cagatgcaga attgtttatc ctcgatccca aaaatgcaga tttagctgat
     9901 ttaggtacgg tgatgcctca tgtttattca caaaaggaag aaatttctgc ttgtgtggaa
     9961 gatttctatg aacgtatgat ggctcgtagc aaggcaatga aagaaatgtc taactacaaa
    10021 acaggagaga attatgccta tcttggactt ccacccaact ttttaatctt tgatgaatac
    10081 gtggcttata tggagatgtt aacgacgaaa gaaagtgcgg tgattttgaa taagctcaaa
    10141 caaatcgtga tgttgggtcg tcaatctggt ttctttctga ttttggcttg ccaaaggccg
    10201 gatgcgaaat atttaggaga cggaatccgc gatcaattca actttcgcgt ggctttgggt
    10261 cgtatgagtg aactcggata ttccatgatg tttggggagg tcgaaaaaaa cttctttatg
    10321 aagcacatca aaggtcgagg ttatgtggat acaggtggca gtgtgattag tgagttttat
    10381 acgccacttg taccaaaagg gtatgatttc ttagattcaa ttagtaaatt agaaaagatg
    10441 aatgattgag tgatcttcaa tactgacttt atacttcaag taacgtataa tacttttata
    10501 tagtaggaag aaaggggata attaatatgg aaagtaatga ttcttattta gagtatgcaa
    10561 aagcaaataa ggataagttt atttcttata ttatcaaagg gaaatccccc gaagttggac
    10621 gagacgcagt ttttatggca gggagtcctg gggctggaaa atcagaggtt gccattggat
    10681 tagctgagaa ttatgataac catgtgatta ttgatgcgga ttactttcgc acccagtttc
    10741 ctgattataa tggaaaaaac tctagtctct ttcaaaaagc atcttcttgg ttggtagagc
    10801 aggcatttag atatgtctta gatcatggct attcttttat tctggatgca acttttgcaa
    10861 tattaagtgc tgaaaaaaat atctcgcggg cgttgaaaaa acattttcga gtgacgattt
    10921 tctatgttta tcaagaccct aaaatcgctt ggaaatttac acaagaacgt gaaattgcag
    10981 agggacgtca tgtgccgaaa aaggcattta ttaacgcttt tttcaaatca agagagaaca
    11041 ttgcaaaagt aaagagtaaa catccagatg tattgcttca tatcataata aaagattata
    11101 cgaataatat ttccgaagtt cattatgcta cggataatct agaattattt ttaccactag
    11161 ggtatagtag gaaagatttg gaggttcaat tacatgactg agaaagaaca agtaacgaaa
    11221 attgtaaaga aatacaataa gagcattgcg gatttatcgg aaaatgctac tgcaaaagag
    11281 ttcaaaacgg tgattaaata tgtagccgat caggccaatg aaaaacaacg gaaattagtt
    11341 ggattaaata aaaaatagta ggacaaatta ataatgtatg agccaagagc aaaatgttct
    11401 tggctttttt ctttgcaatg aatgaggtaa tgtatgagat ttttagattt attcgccggt
    11461 attggtggct ttcgtttagg tatggaacag gcgagccatc attgtatcgg tttttgtgaa
    11521 attgataaat ttgctagacg tagttataaa gcaattcatg atacaagtaa ggaggtggaa
    11581 atgcatgaca tcacaagtgt atcagatgag tttattcaat ccctcggacc agtggacatc
    11641 ctttgcggcg gatttccgtg ccaagctttt tcaattgcgg gaaagcggca aagattttcc
    11701 gatactcgag gtactttatt ctttgaaatc gctcggttca ccactcttct ccgacctaag
    11761 cttttattcc ttgagaacgt ccgaggattg ctcaatcacg aaggaggggc tacgttcgag
    11821 acgatcctcc gaacgctgga tggattgggg tatgacgtgg agtggtaggt gcttaactca
    11881 aaggcctaca ttccccaaag ccgggagcgg atattcctta tcggacattc tcgagacgca
    11941 tgtaccgaac aagtatttcc tatcattgga ccgtcttcaa catctgatca aaacatcaga
    12001 aatttggtaa atatcaatcc ttccaatcga ggaatgggtg gacaagtcta tggttcagat
    12061 ggcgtagcgc caaccttaac aggtgatgaa ggaatcaaaa tcgcactgcc agcaagggat
    12121 ggaatctctg tagcaggact attaccggga aagtttgaac aaggtaatcg agtttatgaa
    12181 gttactggaa ccgcccccac cttgtcaacg aaacaaggtg gtacaaaaat catgattcga
    12241 aaaaatgaaa cagaatatca agaggtgaag ccagaagaca gtgtgaatct ggcttttcct
    12301 aattccacaa gtcgccgagg tcgtctggga aaacagagtg ttcatacctt actaacagga
    12361 gaccaacaag cgatcgtcac ggatcagtat caaattcgga agctaacacc tagagagtgt
    12421 tggcgacttc agggctttcc cgattgggct tttgatcgag attctcaagt caattcagat
    12481 agtcagttgt ataagcaagc cggaaactct gtaacagttc cggtcatttt tgatattgcc
    12541 agaagattaa aagagttgga tgtacatggt ttatgatgag ttgattggag aaatttattg
    12601 ggtgattgga aagattcagt cagacccaga attagaagaa caattgcgac ggttaaaatt
    12661 tgatattcgc aagaatggtg tcaaagtcgc tggcgatcca tatttaatga atgaggagac
    12721 ggatgctcga attgaaataa atcaagtaat tgcagaattt gagcggattg cggaccaagc
    12781 aaaagaacca gatattcgtc aatacttgtt agaaatgaaa gcagagttgg aagtaaatgg
    12841 tattacagat gagtaaccta aaccaacaca actgactgtt aggcgctgac cgcggaggat
    12901 tcgcgaagca gaaatcctcc gtggtgctgt gcctgttcat ggacggcgtt agccgaccat
    12961 gatgtctcac ccccgtatct aacagggggg tacaaaaaca caaaaaataa gtaagcaacc
    13021 attaagaatg cttgttgtat caaagatagt aagtaatttg tataggtgtt aactcatttg
    13081 aaaaagttaa caccttgaat ttggaggtga gaacttggca caacggaatt tagattatcg
    13141 gctattaaaa gaccgacgca acgaatacgg cgtttcacaa aataaattgg ctacggcttg
    13201 tggacttagt cggccgtact taaatcagat agaaaatggt ggcgtaaccg catctataaa
    13261 aaccatgagg aaaatttttg accaactgga aagctttaat cccgatttgc ctttgacgtt
    13321 actgtttgac tatgtaagga ttcgctttcc cacaacggat gcacgaaaaa ttattcaaga
    13381 aattctccat ttgaaatttg agtatatgct ccatgaagat tatgcctttt actcctatca
    13441 agaacagtat gtcatgggtg atattgtggt gatgttgtct catgaagaag ataaaggtgt
    13501 gcttttagaa ttaaaggggc gaggttgccg acaatttgaa acttttttac tcgctcaaaa
    13561 gcgtagctgg tacgactttt tcgaggattg tctaaaggcc ggtggcgtga tgaaacgctt
    13621 ggacttggca atcaatgatc gagtggggct attggatata cctgagctaa cgaaaaaatg
    13681 tcagaaggaa gaatgtatct ccttgtttcg tacctttaaa agttatcgtt ccggggaact
    13741 tttaaaggct gatgaaaagg acggcatggg caatacctta tatatcggca gtctcaagag
    13801 tgaggtttat ttctgtttgt atgaaaaaga ctatgaacaa tatatcaagt tagggattcc
    13861 attagaccaa actgagacga aaaatcgttt tgagattcga ctgaaaaatg accgtgcttt
    13921 tcatgcgatt caagatttgt taaaaggtcg tagcattgag agcaccacgt tttccattat
    13981 caatcgttac ttgcgatttg cggataaagt agaaggtaaa aggcgaacca actggccctt
    14041 gaatgaacaa tgggggcgtt ttattggacg gaatcggaaa gagattcaat taacttccga
    14101 gccaaaaccg tataccattg aacgaacctt gaattggcta ggccgacaag tcgcccctac
    14161 gtggaaaatg gctaaggagt tggatcgatt gaatcggaca acctatatcc aggatatggt
    14221 acagaatgca cgcttatcag accgacataa aaagattttg gagcaacaaa gtatggcaat
    14281 tgaaaatttg attatatgaa aagaggaatc gtcatgggaa ttgtaaaaga tatttttttg
    14341 gtatttggtg gcagtatgct tggtgttact gtgatgtgct tgatgaatgc aagtgcagca
    14401 gcggatcgag caatggaaaa gaaaggaaag tgacaagatg aatttcggac aaaattt
//
LOCUS       DAZKBH010000067        14341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000067 DAZKBH010000000
VERSION     DAZKBH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.067"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..81
                     /locus_tag="ACG8OO_001894"
     CDS             <1..81
                     /locus_tag="ACG8OO_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFP9045764.1"
                     /translation="DTIVLHQRKLDLLKEQKKGFLQKMFV"
     gene            108..488
                     /locus_tag="ACG8OO_001895"
     CDS             108..488
                     /locus_tag="ACG8OO_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045765.1"
                     /translation="MKFLNFLNYKYLNWVLYGITLFFIIYYFFTDDDIFLILAILFLV
                     SSNFSDNLIEVLTEPEIRSMTDEEQQIIKAMISENKKDVVVIKKIREYTNLGVLQAKK
                     VYEKKKRVYNYKLDNTLEAGDKIV"
     gene            619..1569
                     /locus_tag="ACG8OO_001896"
     CDS             619..1569
                     /locus_tag="ACG8OO_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9045766.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2087..2497
                     /locus_tag="ACG8OO_001897"
                     /pseudo
     CDS             2087..2497
                     /locus_tag="ACG8OO_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2940..3752
                     /locus_tag="ACG8OO_001898"
                     /pseudo
     CDS             2940..3752
                     /locus_tag="ACG8OO_001898"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3747..3911
                     /locus_tag="ACG8OO_001899"
     CDS             3747..3911
                     /locus_tag="ACG8OO_001899"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045767.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            3851..3973
                     /locus_tag="ACG8OO_001900"
                     /pseudo
     CDS             3851..3973
                     /locus_tag="ACG8OO_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(4535..5041)
                     /locus_tag="ACG8OO_001901"
     CDS             complement(4535..5041)
                     /locus_tag="ACG8OO_001901"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9045768.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RYWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRNSKILVPINILKSKISSLVSPLTEEL
                     QKIAMKVM"
     gene            complement(5046..5813)
                     /locus_tag="ACG8OO_001902"
     CDS             complement(5046..5813)
                     /locus_tag="ACG8OO_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9045769.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6303..6728
                     /gene="lacA"
                     /locus_tag="ACG8OO_001903"
     CDS             6303..6728
                     /gene="lacA"
                     /locus_tag="ACG8OO_001903"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFP9045770.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6745..7260
                     /gene="lacB"
                     /locus_tag="ACG8OO_001904"
     CDS             6745..7260
                     /gene="lacB"
                     /locus_tag="ACG8OO_001904"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFP9045771.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7271..8203
                     /gene="lacC"
                     /locus_tag="ACG8OO_001905"
     CDS             7271..8203
                     /gene="lacC"
                     /locus_tag="ACG8OO_001905"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFP9045772.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8206..9186
                     /gene="lacD"
                     /locus_tag="ACG8OO_001906"
     CDS             8206..9186
                     /gene="lacD"
                     /locus_tag="ACG8OO_001906"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFP9045773.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9214..9531
                     /locus_tag="ACG8OO_001907"
     CDS             9214..9531
                     /locus_tag="ACG8OO_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9045774.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9531..11237
                     /locus_tag="ACG8OO_001908"
     CDS             9531..11237
                     /locus_tag="ACG8OO_001908"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFP9045775.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11379..12785
                     /gene="lacG"
                     /locus_tag="ACG8OO_001909"
     CDS             11379..12785
                     /gene="lacG"
                     /locus_tag="ACG8OO_001909"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFP9045776.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12808..13080
                     /locus_tag="ACG8OO_001910"
     CDS             12808..13080
                     /locus_tag="ACG8OO_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFP9045777.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13101..14000
                     /locus_tag="ACG8OO_001911"
     CDS             13101..14000
                     /locus_tag="ACG8OO_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP9045778.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4684 a         2363 c         2765 g         4529 t
ORIGIN      
        1 gacactatcg ttcttcatca acgtaagtta gatttgttga aagagcagaa aaaaggattt
       61 ctacaaaaaa tgtttgtttg atattttaaa tagaaaagag agataaagtg aagtttttaa
      121 attttttgaa ctataaatat ttaaactggg tattatatgg tattacatta ttttttatta
      181 tttattactt ttttaccgat gatgatattt ttttaatttt agcaatactt tttcttgttt
      241 catctaattt tagtgataac ttaattgaag ttcttacaga accagaaatt cggtctatga
      301 cggatgaaga acaacagatt attaaagcta tgatttcgga aaataagaaa gacgtagtag
      361 ttatcaaaaa gattagagag tatacaaatt taggtgtttt acaagccaaa aaagtatatg
      421 aaaaaaaaaa aagggtctat aattacaaat tagataatac attagaagct ggagacaaga
      481 ttgtctagat aatgtgtgag ataaaatttt tggcaggtta tcatacataa gacaaaatgt
      541 ttttaaaact gataacgtat ttattccaca acaattacgc atttatttct gagaaaaata
      601 taaagcagga ggattacaat gctgaaaact tattccttaa caaaagatac attattagtt
      661 tcgtcagaat caaatatcaa aaattcttct gttgtttatg tttatggtga cgaacgagaa
      721 tatatcgaat cttctgaaca taaatatgac tttaaaatag gcaatatact aacgtttgat
      781 gatcgagttg catatgacac gatgatagat aagaacgatg aaaaaagttt gctcgtaagt
      841 tttttgtttc ccgaggtaca tgaaggtggg catctagata ccctgacgac atctgttgtt
      901 ttcttggtat ttaaaaataa gttgataatt tttacaaaac aaaaattaaa attcattccc
      961 gaactcttat ccaatatggt tttacgtgat gtcaatcatt tcgtggagga agtactttta
     1021 aagatcatgc aaaaagattt ctatatgatg cttaacgacc ttcgtggagt caaggaaaaa
     1081 attgatgatt tagactcaga aatatcaacg tccggttcac tcaaaccaac gtttggtgat
     1141 ttattgactc ttcaaaaata catgatagct ttgtcaacaa cttataaagc taaccataaa
     1201 gcgcttgatt tcattaaaaa aaatttcaca actattgacg atattgattt tagtactaaa
     1261 aaaataattg atgatttata cgatacaagt gatacaatgg atagaatcat tttaagttac
     1321 agccaatatt tggacaaatt ggaaaatatg ataaacaata tgatttcgta tcagctaaac
     1381 atgattatga aaaccctaac cgagatatca attgttttga ctattccagc aattattttt
     1441 ggcttttggg gaatcaatgt ccatgtcccg tttgaaaagt cttcttatgg tgttttagcc
     1501 gtattgatta tctctgctat actaagtcta atttgctggc ttttgatgcg acgcaaaaca
     1561 tatttgtaaa gtatttgtga taccctaata tgatttaaag cagaatttct ttgttgactt
     1621 tttaaaaggc tgattccttt caataattta atagcttcag cggctgctca gaagtcaata
     1681 aattggacta tttttattca gttacttagg caacatttga gatacgattg cgattataaa
     1741 aattgatata ccaaccaatg gctgcttgga agactgacat attagcctcg atatcatcga
     1801 ataactaaat acagaaaaat acaataaata ttatcaaact agggactata ctagaaaaca
     1861 tcagtacatt ccttaactca gggtctataa ttagaaaatc gcccctttga aaactgatta
     1921 aaacagcccc cattatctag tgactagata atgggggctg tttttttgtt aagtgatata
     1981 ataaaccata aaagaagttt agaaatacgg tcctggtcat caataaataa acaatttaat
     2041 taattattta tttaattatt taatttcaga aaggtataca tgaatcatgg tgcaacaaat
     2101 tgtcctaccc atcaaagact ccagcgtctt aaagatggta caagatacac ttctcgatag
     2161 tttccgtgct ggtcgccgaa attacaccgt ttttcaagtt ggtaaggcca cgctactacg
     2221 tgtgagtgat gtcatgacat taaaaaaatc agatgtctat aatccagacg gctctgtcaa
     2281 aaatgcaacc tttattcatg ataaaaagac cggtaaagca aacacgttat atttaaagcc
     2341 tgttcagcaa gacttgttgc aatatcatga ttggctggcc caagaaaata tcaattctga
     2401 gtggttattt ccctcgacag cccattatga tcttcatatc actgagaagc agtttcataa
     2461 ggtgatggcg catgttggtg atctcttagg aatcaacttg gaagacaaag agaaaaaatt
     2521 gcgagtcaga aaaccgtttt gagatatgaa cagcatttgc cttcacgcct tagcgatttt
     2581 aaataatagc cttgaaagtg attatggaaa agccctctac gctcaatgca aaattgacgt
     2641 agaaactatt tttgatcgat tgaaaggtgt atttggtatg tgaagaaccc atgtcagagg
     2701 caagaacgtt gcacctactg acattggaat gatgcttatg agcatgaatc taaccaaatt
     2761 atcccttgag gctagaagaa aagcggaagc ttttcaacac aaagcagcca aaaataaaaa
     2821 acgcgacgaa gcaatcacat ttatgattat tcatcgcgtt ttttatattt agagctagtt
     2881 atttgctaga cccactattt acagatgaaa cgtttatcac ctttggtttt gggcatttag
     2941 tggaattagc agaaccaggt cattatgacg aaaagtggca aaattggaaa cttgaatctt
     3001 tgccgatttt tcctgatcga tacgattttg aagttgcaaa agataaggga aagcagttta
     3061 aaattgttgc agaacttctc aaaaaggcaa atacaattat tgttgcaaca gatagcgaca
     3121 gagaaggtga aaatatcgcc tggtcgatta tccataatgc aaatgccttt tcaaaagata
     3181 aaacatttaa aagactatgg atcaatagct tagaaaaaga tgtgatccga agcggttttc
     3241 aaaatttgca accaggaatg aattactatc ccttttatca agaagcgcaa acacgccaaa
     3301 ttgccgattg gttgatcggc atgaacgcaa gccctttgta tacgttaaat ttacaacaga
     3361 agggcgtaca aggtacattt tcactaggac gtgttcaaac gcccacctta taccttattt
     3421 ttcagcgcca ggaagccata gagaatttta aaaaagaacc ttttttcgag gtggaagcta
     3481 agtaaaacaa gggtcattta agggcgttct aagccccaca cagcgtttta aaacccaaga
     3541 ggagcttttt agcttttgtt tcttctaaac aagctaaaat aggcaatcaa gaggggataa
     3601 ttgctgatgt tcaaaccaaa gagaagaaaa cgaatagtcc gagttcgttt tctttaagta
     3661 gtttgcaatc aaaagtcaat cagctttata aagcgacagc gagccaaact ttaaaagcta
     3721 tgcaaggact gtatgaagca aaattattga gttatccaag aacagataca ccatttatta
     3781 cagagaacga atttgcttat ttaaaagcga attttggcaa atatagcggt tttttaggac
     3841 ttgatcttga aatgttgtta tcattgataa tgatacgttc aaacaacagc acccaaattt
     3901 tgacgaatta gtgaaactct atgaaaaaga cgtagtaaaa catgcaacat cctattctaa
     3961 tcagctggta aagcttaatt aggttatata agcttgaaaa taaagggttt gtccaatatg
     4021 gacaagcctt tttcttatgt tttgccacag atttgccaca cttattgtgt aagagtatca
     4081 aggataggta taatttgata ctttgttttt ttagtgatgt gcaaataaat ttctgttaca
     4141 cgactacctc tagaatgccc tacacggtct tgaattgctt ctaggggtat tccttactct
     4201 gctaagaaac taatatgcgt atgacggaat atatgggtcg taatatgttt attaaaacta
     4261 agtttattat caatatcaat tcctgctcgt tttggattac cttttcgatt taattttcca
     4321 ttttcaggtg agttgaggaa attgttaagg acactataat gaagaggtgt tccattatag
     4381 tcgggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
     4441 aatctcatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
     4501 gataattgga catttcggta actttgtaag ttttttacat taccttcata gctattttct
     4561 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
     4621 gcaccaatat tttggagttt cgactctgta agtcaacagg gccattagtt agaataccta
     4681 ttgggatgcg gtttttatgt agagccagca gtagttgatg tatctcagga atcatcgtaa
     4741 tctgatcgag ttcatattca tagtcagctt ggaagtctag agcttctgtc ctagaaatac
     4801 tatcgatgcc aaattcttcc atagtcttct gacagcgaaa aattctaagc tgctcaatac
     4861 tgatgagttt tttcgtgtat ttaggaaaag caacatcaga ccaatatctg aaacgtttgt
     4921 aaatgttatc tatttgttgg atatcaatcg ttggaaaaca tttctcaacg ctacgtttaa
     4981 aggggagttg atggtcataa agagtgtcat caaggtcaaa aacaatattt ttataagtca
     5041 taattctagt ccgcttttaa aatagtagtg tactggctaa attcctctaa gtgttgaggg
     5101 ctaatctggt tatctgtaat gatggtatcg agttgatcta gattatagaa gttaaagaaa
     5161 tcgtatcgat cgaatttagt actgtctact aataagaatt tttctacagc attgtttagg
     5221 gcaagttgtt gaatcacacc ttccttgtca ctatatgtag caatagaatt atgggtaaca
     5281 gcatttgcac taacaaaagc tttggcaaat ctaattgctt ttaaatttgt cgaagccatt
     5341 gaacctacaa aagctccagt tatttctcta tattcaccgc caagaagcaa taaatcaatg
     5401 gtttcgctat catttagaat caagaacaca gggagactat ttgtaatgac acgaatttta
     5461 taacctttac gacctttcaa ttctaatgcc agttgttcaa gtgtagttcc aggtccaata
     5521 aaaatagtat cgccatcttt gattaaagag caggcttttt tagcaatgtc aattttttct
     5581 tttgtattta gacttttctt ctcgatatgt gtcttttcaa gtggcttttt agaggaaagc
     5641 aattgtgcac caccatgagt acgtgttaaa agtccatcag cttctagagc atcaaggtct
     5701 ctacgggctg tcatatcgga aatatctagt tcatctatta tttcttttat ggttatggtc
     5761 ccatcaatct ttaacatatc taaaattttt tctaatcgtc gttttttgtt catatgaaga
     5821 cccttctttc ataaagtaat ttttttccaa agataattct cttttaattg tatcataaaa
     5881 gacaatattt tcaaggtaaa acaaacaatt tcaaacaaaa acaaatgtta gatgatgaaa
     5941 taagaacaga ggattgacgt atattagctt aggtcagatt ttgtataaga cgaaaataaa
     6001 gtaggacctc ttaatcagta agttatagaa agtaaaagac ttttgtaata cttgaataga
     6061 tatttcacgt ccattttgtg atggattaaa tgaacaaaaa tgaacaataa tttaacggtg
     6121 ttatctattt tttaaaaaaa caaataaaaa aaacaaaaaa taaacaaaaa aagtgttgct
     6181 ttttgtttga atgcttgata ttatataaac aaagaaatga tgaaaacgtt ttttcgaaca
     6241 ttttgcaaaa tgttttctac ttctacgtag catttctttc taaaatttag gaggtggtca
     6301 aaatggctat tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa
     6361 attttcttgt agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg
     6421 ttgatgttac cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg
     6481 taattgatgc ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg
     6541 cagcagaagt ttcagatgaa cgctcagctt atatgacacg tggacataac aattcacgaa
     6601 tgattacggt tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaaggtt
     6661 tcgtcaatgg taaatatgat ggcggacgtc atcaaatccg agtagatatg cttaacaaaa
     6721 tgtgttaaga aaaggagtta aggaatgaga atcgcaattg gatgtgacca cattgtcaca
     6781 gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag gacatgaggt ccttgatttc
     6841 gggacatatg accacgcacg tactcactac cctatctatg gtaaaaaagt cggtgaagca
     6901 gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg gtacaggtgt cggcattaac
     6961 aatgcagtca ataaagttcc aggtgttcgc tcagcgttgg ttcgtgatat gacatcagct
     7021 atatatgcaa aagaagagtt aaatgcgaat gttatcggtt tcggtggaaa gattactggt
     7081 ggtcttctta tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa
     7141 gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca
     7201 gatgaagatt tctttacaga attccttgaa agatgggacc gtggtgacta ccacgattaa
     7261 ggtataacaa atgattctga cagtcacact aaatccttca gtagacatct cttatcctct
     7321 agaaacattg aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg
     7381 aaaaggctta aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg
     7441 tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag
     7501 tccagctttt tataaaatct caggaaacac tagaaactgt attgctatct tacacgaggg
     7561 taaccaaaca gaaattttgg aacaaggacc aacaatttct catgaagaag cagaaggctt
     7621 tcttgaccac tatagcaatc ttattaaaca atcagaagtt gtgacaattt caggaagttt
     7681 accttcagga cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg
     7741 agtagcagtc gttttggatt gttcaggtgc acctcttgaa acagttctaa aatcgtcggc
     7801 taaacctact gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt
     7861 aacaaaagat attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga
     7921 atggatagtg gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta
     7981 taaggtagat attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt
     8041 agctggtatt gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc
     8101 gatgacattg ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa
     8161 ttacgaaaca ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa
     8221 cagaaacgca aatctttgga aaaaatttca gataaaaacg gtgttatctc agctttggca
     8281 tttgaccaac gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact
     8341 gtagctcaaa tggaagaact aaaggttttg gttgctgatg aacttacaaa atacgcttct
     8401 tcaatgttgc ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca
     8461 ggtcttcttc ttgcctatga aaaaacaggt tatgatacat caagtacaaa acgtttgcct
     8521 gactgcttgg atgtttggtc tgcaaaacgt atcaaagagc aaggcgctga tgccgttaag
     8581 ttcttgcttt actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat
     8641 attgaacgtg ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt
     8701 gcttatgatg aagaaatctc agatgcaggt tctgttgaat atgcaaaagt gaaacctcgc
     8761 aaagtcattg aagcgatgaa agttttctct gatccacgct ttaacattga tgttcttaaa
     8821 gtagaagtac cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtagtttac
     8881 agtaaagctg aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac
     8941 atctacctaa gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac
     9001 gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta
     9061 gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc
     9121 gaaaatattg atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa
     9181 gtatagtaaa aacataaaac ggaggatatt gttatgaaca gagaagagat gactctctta
     9241 gggtttgaaa ttgttgctta tgctggagat gctcgctcta aacttttaga agcgcttaaa
     9301 gcggctgaaa atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt
     9361 attgcagagg ctcacaattc tcagacagct atgttggctc gagaagcttc tggggaggaa
     9421 cttccataca gcgttaccat gatgcatggt caggaccact taatgactac tgtcttatta
     9481 aaagatgtga ttcatcacct catcgaactt tataaaagag gagcaaaata atgcataaac
     9541 tcattgaact tattgagaaa gggaaaccgt tctttgagaa aatctctcga aacatctatc
     9601 ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt catccttttc tcatctatct
     9661 ttatccttat tgcctatgtc ccaaatgctt ggggattcca ctggtcaaaa gacattgaga
     9721 ccttcttgat gactccttat agctattcga tgggtatcct agcattcttt gttggtggta
     9781 ctaccgctaa agctttgacg gactcaatga accgtgacct acctgcgacc aatcagatta
     9841 acttcttatc tacgatgcta gcgtctatgg tagggttcct tttgatggca gctgagcctg
     9901 caaaagaagg aggcttcttg acagccttca cgggaacaaa aggtcttttg acagcattta
     9961 ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt gaaaaataat gttactattc
    10021 gtatgcctga agaggttcca ccaaatatct ctcaagtatt taaagacttg attccattca
    10081 ctgtagcggt tgttctcctt tatggatttg aactcattgt taagggaact cttggtgtaa
    10141 ccgttgcaga atcaatcggt acccttcttg ctcctctctt ctcagctgca gatggttacc
    10201 ttggaattac acttatcttt ggtgcttatg ccttcttctg gtttgttggt atccacggtc
    10261 cttcaattgt cgaaccagca atcgctgcta tcacttatgc caatatcgat gccaacttgc
    10321 atcttatcca agctggacaa catgcagaca aagttatcac ttctggtact caaatgttta
    10381 ttgttaccat gggtggaaca ggagctacat tgattgttcc attcttgttc atgtggattt
    10441 gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt tgttccaaca ttctttgggg
    10501 ttaatgagcc aatcttattt ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt
    10561 tcattttcgc tccgattgtc aacgtttgga tctttaaatt ctttgttgac acattgaaca
    10621 tgaactcatt ctctgccaac cttccatggg ttactcctgg tccgttggga attgtactcg
    10681 gtactaactt ccaagttcta tcatttatct tagccggact cttggtagtt gttgatacta
    10741 tcatttatta cccatttgtt aaggtatacg atgaacaaat tcttgaagaa gaacgttctg
    10801 gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa cttcaatact gctaaagcag
    10861 atgccgttct tggaaaagca ggcgttgcta aagaagatgt agcagcaaac aataatatca
    10921 caaaagaaac taatgtcctt gttctttgtg caggtggagg tacaagtggc ttgcttgcca
    10981 atgccttgaa caaagcagca gcagaataca atgttccagt caaagcggca gcgggtagtt
    11041 acggtgctca ccgtgaaatg ttgccagagt ttgacttggt tatcttggct ccacaggtcg
    11101 cttcaaactt cgatgatatg aaggctgaaa cggataaatt gggcattaaa cttgcaaaaa
    11161 ctgaaggagc tcaatatatc aaattgacac gtgatggaca aggtgctctt gcatttgttc
    11221 aacaacagtt tgattaataa atttgtaaaa gttatttgag acgagattat tctttcaatt
    11281 ccttataata ttgttaacca tatcattgca tttcatttcc ttggtttccg gaactcagaa
    11341 tatcgtctca aatgcttttt ttgaaaggat ttatagttat gactaaaaca cttcctaaag
    11401 attttatttt tggtggtgct acagctgctt atcaagcaga aggtgcgact cacacggatg
    11461 gtaaaggccc agttgcatgg gataagtatc ttgaagacaa ttattggtac acagcagagc
    11521 ctgcaagtga tttttaccat aaatatcctg ttgatttaga attagctgaa aaatatggcg
    11581 ttaatggtat tcgtatttca attgcctggt ctcgtatttt cccaactggc tatggcgaag
    11641 taaatgaaaa aggtgttgaa ttctaccata atctttttgc cgagtgccac aaacgtcatg
    11701 ttgagccatt cgtgactttg caccactttg acacaccaga agttcttcac tcagatggag
    11761 atttcttaaa ccgcgaaaat atagaacact ttgtagatta tgccgctttc tgttttgaag
    11821 agttcccaga agtaaactac tggacaactt tcaatgaaat tggcccaatt ggagatggtc
    11881 aatacttggt tggtaaattc cctccaggca ttcaatatga tcttgccaaa gtcttccaat
    11941 cacaccacaa tatgatggtt tctcatgcac gtgctgtgaa attgtataaa gataaaggtt
    12001 ataaaggcga aattggtgta gtacatgcgt tgccaaccaa atacccttat gatccagaaa
    12061 atccagcaga tgttcgtgcc gctgagttag aagatatcat ccataacaaa tttatcttgg
    12121 atgcaaccta ccttggacac tattcagata aaacgatgga aggggtcaac cacatcctag
    12181 ctgaaaatgg tggagaactt gatcttcgtg atgaagactt ccaagcactt gaagcagcta
    12241 aagatttgaa cgatttcctt ggtatcaact actacatgag tgattggatg caagcctttg
    12301 atggtgagac tgaaatcatt cacaatggta agggtgaaaa aggaagttct aagtaccaga
    12361 ttaaaggtgt tggtcgccga atcgcaccag attatgtgcc tagaacggat tgggactgga
    12421 ttatttatcc tgaaggcttg tatgaccaaa tcatgcgagt gaaaaatgat tatccaaact
    12481 acaagaagat ttacatcact gaaaacggtc ttggatacaa agatgagttt gtagataata
    12541 ccgtttatga tgatggtcgt attgattacg tgaagaaaca cttggaagtt ttatcagacg
    12601 caattgcgga tggtgcaaat gttaaaggtt acttcatctg gtcacttatg gatgtcttct
    12661 catggtcaaa cggatacgaa aaacgttatg gactattcta tgtagacttt gacacgcaag
    12721 aacgctatcc taagaaatca gctcattggt ataagaaagt agcagaaact caagtgatag
    12781 agtaatatta cttgaaaatg taaaaaattg ttatccgata ctttagaaaa agtacaacac
    12841 ttatttagac caggctttga aaaggtatat attgttagtt tcgaagattg tcctatgatt
    12901 cctgagcttg aagctacccc gttgctaaaa tgtggtaaat ggtatgtttc aactggaaaa
    12961 gagtggattt gccattctga tttagagtta tcagcatttg aatgggaatt tttacaatca
    13021 ctcgatgtag aaatacgtga gactattcac ttcgaggtaa attatttgcc tttccaataa
    13081 taaaaagaaa ggaattagtt gtgactatcg aattaaaaaa cgagtatctt actgttcagt
    13141 ttaaaacact tggtggtcaa ctgacttcga ttaaagacaa ggatgggata gagtatctct
    13201 ggcaagctga tccaaattat tggaatggac aggcgcctat tctatttcct atctgtggta
    13261 gtttacgaaa tgattgggct atttatagac ctcaagaaag accctttttt acaggtctta
    13321 ttcgtagaca tggttttgtt cgaaaagaag aatttattct ggaagaagtc aatgataata
    13381 gcgtgacctt tagtattaaa ccaaatgctg agatgcttga taattaccta tatcagtttg
    13441 aactgagagt tgtttatacc ttgaatggta aatcaattag aaccgaattt caggtaacga
    13501 acttggaaat ggaaaaaaca atgccttact ttattggagc ccatccagca ttcaatagtc
    13561 ctttagtaga aggtgaaaac tatgaggatt attatattga atttagtgag gttgagtctt
    13621 gctcgatacc aaagagtttt cctgagacag gattattaga tttgcaagat cggacacctt
    13681 ttttggaaaa tcaaaaaatt ctcgatttgg actactcact tttttcccat gatgccatta
    13741 cacttgacca gctcaagtct cgtagcgtaa cgcttcggtc aaggaagtct ggtaaaggtc
    13801 ttcgagtaga ttttgatgat tttccgaact tgattctgtg gtctacaact aataaaagtc
    13861 cattcattgc tttggaacct tggagtggtc tctccacttc acttgaagaa ggtaatttct
    13921 tagaagataa aagacaggta acgaaaattt cacctcaaga aacttctaga aaaatctatg
    13981 atattactat ccttaattaa aatttgttat tatttgagtc ttcaataaga tttgaatact
    14041 cttaccttgt gatgatagat tggtcttctc cgtgccagtc ttacatcgca aggtttttaa
    14101 gttttttgtg ggtctatgtc aactgtagtg ggtgacgaaa agctaatatc taaagacagt
    14161 ctggtagtaa aaaataagat tatttactac ttcttgattt aaaataatag tggagccatt
    14221 aatttgtggc aaatttaatt taatattttg cctcaaatta gccacacttt ttatagaact
    14281 aagaggaaaa atgaaatttt cttcctatta atatagttgt ttgaaatctt aaatatagcg
    14341 t
//
LOCUS       DAZKBH010000068        13893 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000068 DAZKBH010000000
VERSION     DAZKBH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.068"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(424..1371)
                     /locus_tag="ACG8OO_001912"
     CDS             complement(424..1371)
                     /locus_tag="ACG8OO_001912"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFP9045779.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1561..1830
                     /gene="rpsN"
                     /locus_tag="ACG8OO_001913"
     CDS             1561..1830
                     /gene="rpsN"
                     /locus_tag="ACG8OO_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9045780.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(2006..2368)
                     /locus_tag="ACG8OO_001914"
     CDS             complement(2006..2368)
                     /locus_tag="ACG8OO_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9045781.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2379..3500)
                     /gene="alr"
                     /locus_tag="ACG8OO_001915"
     CDS             complement(2379..3500)
                     /gene="alr"
                     /locus_tag="ACG8OO_001915"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9045782.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3516..3866)
                     /gene="acpS"
                     /locus_tag="ACG8OO_001916"
     CDS             complement(3516..3866)
                     /gene="acpS"
                     /locus_tag="ACG8OO_001916"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9045783.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3996..5507)
                     /locus_tag="ACG8OO_001917"
     CDS             complement(3996..5507)
                     /locus_tag="ACG8OO_001917"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9045784.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5829..7181)
                     /gene="murF"
                     /locus_tag="ACG8OO_001918"
     CDS             complement(5829..7181)
                     /gene="murF"
                     /locus_tag="ACG8OO_001918"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9045785.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(7334..8401)
                     /locus_tag="ACG8OO_001919"
     CDS             complement(7334..8401)
                     /locus_tag="ACG8OO_001919"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9045786.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSADKIMTKYILQAAGVPQVPYVPVLKNQWKENPKK
                     VFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGIEAR
                     EIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQEYA
                     KLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGDLIE
                     ELIQLGMNRYHQRQSFFEKNE"
     gene            8597..10342
                     /locus_tag="ACG8OO_001920"
     CDS             8597..10342
                     /locus_tag="ACG8OO_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFP9045787.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10622..11059)
                     /locus_tag="ACG8OO_001921"
     CDS             complement(10622..11059)
                     /locus_tag="ACG8OO_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9045788.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(11043..12416)
                     /locus_tag="ACG8OO_001922"
     CDS             complement(11043..12416)
                     /locus_tag="ACG8OO_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045789.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12738..13226)
                     /locus_tag="ACG8OO_001923"
     CDS             complement(12738..13226)
                     /locus_tag="ACG8OO_001923"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9045790.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(13248..13850)
                     /locus_tag="ACG8OO_001924"
     CDS             complement(13248..13850)
                     /locus_tag="ACG8OO_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9045791.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3983 a         2997 c         2510 g         4403 t
ORIGIN      
        1 acgcatatag cgaagggatg atcatgatcg cgatgatcag aagtgttgca atcgggtttt
       61 tgaaaatccg tttccagtca agcaaatata atcgaagcgt atttttgatt gactgcatag
      121 ttctccccct tcttcttttt tcagctgaat tactttacca tgtatttcat acttttttca
      181 atcggctttt ttcaaaaaag tcgaattttt ggacatatca cggcaattgt ctcattgtaa
      241 tctcttacat attgagaaat aattaaggat tacagccttt actttatcat gaaataaaag
      301 ggaaagataa ttttatgcca aagtttttat ctattatacg tatacgcaaa aaagctaaaa
      361 cattttttgt ctttgcccct acaaaaatgt ttcagctttt ttcgtttttt attcctgtat
      421 ttcttatttt tcgaatgaca ggatgatttt cccagcagga ggatgtcctt caactagctg
      481 atgtgcttgg atgacatgct taagtgaagc agacagattc attgaaataa aaacatgaaa
      541 ggctcctttt tggtaggctt tgattatgcc agcaaaggct tctgcatcta gatattcctt
      601 tcttggaaca aacgattcat aaaagccttc ctttttttgc cgcaaatcta gatctgggag
      661 gtcatttaat gcaacatacc tgccgcctgg tttcatgatc tggatgccgg tttctccttt
      721 gatacttcct ttcgtcgcat cgattacaag gtctgcctga tcagcaaatt gcagacctgg
      781 atttgtttta tcataagcag caaatgctga agctcctaat ttttttacct tttcctcatt
      841 tttactggat gcactggtta agattcggat tcccttttca tggagaagct ggatcagact
      901 tgacccgacc gcacctgatg ctcctagtat cattacagta tctgtcggct ggatttccgt
      961 caaatggttg attagattat aagccgtaat tcctggcgtg accattccgg ctgcttcttc
     1021 ccaactcatt ttatctggaa tttttgctac ttttgctgat ggcaacacga cttcttctcc
     1081 gtaagtcccg ccgactgcat ggacagctac tcgatcacct acatgaacag ttgtgacatc
     1141 tgaaccaatc tctgtcacaa taccagcgcc atcattacct ggtacataag ggaatttcaa
     1201 tgttcgtatc tctttcattt ctcctaaacg caaggcaaca tcataaggat tcacactgaa
     1261 tgcttttatt tctacacgaa catgtccaga accgacttct ctgggatgag catcgatttc
     1321 ttcaaaaaca tcttgtgctt ctccgtaacc gttaattgca aatcttcgca tggtcaccat
     1381 ctcccttttt ctttaagttt acgtttatgc ctttattttg acaaaaaaaa tgtcttttcg
     1441 ttttggcgca tgattcaaaa caaaatagtt gtcttttttc gcgtaacgtg ttaaattatt
     1501 tatctaaaag cgtaaagatt acgatttacg ctaataaaaa ataattggag gaatttgttg
     1561 atggctaaaa aatcaaagat tgccaaagca aaaaaacagc aagaaacgat tgaacgctat
     1621 gctgctttac gccaggaatt aaaagcgcaa aaagattacg aaggcttgcg taaacttcct
     1681 ttagatgctc gtccagaacg attgaaaaat cgtgatttaa tcgatggacg ccctcgcgga
     1741 tacatgcgta aattcgggat gtcccgtata aaattccgtg aattagcaca taaaggtttg
     1801 attccaggag taaaaaaagc cagctggtaa tccaagtctg gctgtaatgt aaaggaggag
     1861 ggcgcatgga ccactccttg gatcatcaaa aaagatacgt tcgcttcttc tagcagaaat
     1921 gctggtacta gaagcagacg tatctttttt tacgcacagc tggtcctttt ttatatgtaa
     1981 aggctgcatg gttacttcgc tatatttatt tagtaacagg agtcagtgct tcgataccca
     2041 cactaatttt caatgctcgg tcaacttctg ccataatggg tcgatccaaa tggcagacct
     2101 tttctttcaa tcttgattta tcgatcgttc tgatctgctc caacaggata acggagtctc
     2161 tttctattcc tgttccagta gctttgatcc caatgtgagt cggaagctta ggtttagcca
     2221 tttttgccgt tatggccgca acaatgatcg tcgggctaaa atggttacct agattatttt
     2281 gaataaccaa aactgggcgt gttccgcctt gctccgatcc cacgactgga gaaagatcag
     2341 caaaataaat atctcctctt tttaccatag gatcctcctt attcttgata ttctcttggt
     2401 atccgctgtc ctaatccgca agccacttca tagtggatcg tacctaattg atctgctata
     2461 tcctgcatag tgatttcttt gtctgcattt ttgccgataa gcgtcacttt tgttccgaca
     2521 ggaaattctt gaggcaatcg gatcatcagt tgatccatac atacacgccc aatcgtttca
     2581 cagtagttcc cttctaccaa tagagaaaaa ccctgcatct ttcgtggcca gccatctgca
     2641 taaccgatcg gtatcgttcc gatccactct gcttcaggag tgatatatgt ttctccatag
     2701 ccgattcctt cacctgctgg gagttttttg acttgaatca attcagaaac cagctcaagt
     2761 gctggctgca atggataaac ttccggtagt gcgtgcccag agggattaag tccgtacatt
     2821 gctactccat aacgaatcat attgccgatt gtttcatcat gccataaagc cgttgcacta
     2881 ttgctgacat gtacatatcg aggaagttcg ggaagttttt tcagcacttc tataaagcgt
     2941 tctttttgca gattccaata tgtatcatct gcctgatctg ctgtagaaaa atgggtaaat
     3001 aaaccttccc attctaatac acgactttct ttgatgaatg cagctgcctc ttttacttct
     3061 tcaggtgtac aaaatccgat acgacccatc ccagtgtctg cttttagatg aatcgaaaga
     3121 ggcgtctcta gcttactttt ctctaaaaca gcagctgctg cttgcaacca ttcccgtgta
     3181 cctgcagtta ctgataaatc atgagcaaca gctacagtca gtgcgtcgta aggaaccata
     3241 ttcaagataa ggatgggctg agtaaatcca gcttctctca actcgactcc ttcatctaaa
     3301 tttgaaacac aaaagcccga agctccacct gcaactgctg cctcagctgt ttcaatagct
     3361 ccatgaccgt atccgttcgc ttttactact gcaaacaatt cttttccttg cggaagtctt
     3421 gcaacttcat ttgctacatt ttcagttata gcttttttat gtatcactgc tttcgttggg
     3481 cggtgccaag cgacaaccat cgtttttcct tctttctagt cttccaaaat aatttgtgca
     3541 aaagctgttt catttgtatg agtaatactg acccatacat tgccttcatg aggtgaacgc
     3601 gtcacaacag gctgaccatt ttcatttttt aaaatctcga catcttgaaa ggtaactttt
     3661 ccgatcccag ttccccaagc tttagagaaa gcctctttgc aagcgtaacg cccgcctaaa
     3721 aattctactt ggcgatgaaa aggcaaggac tggaatagtt tcatttcatc agaggtcaag
     3781 attcgtgcaa tgaattttgg tttttcttcg atcagccgag tgatcctagg aagttcgact
     3841 gcatcgatcc caattccttt tatcatatgc ttttctcttt tcaataaatt tagaacactc
     3901 tcattctacc aaattataac cattttgcca caaataagtc cacaaataac aaaaatgaaa
     3961 gtttctctta aaaaagagaa actttcattt tatgtttaat cgttatttga acggattaca
     4021 aatccgcgtt ttttatcatt gtgacgttta ccgccatctt tttggctgtt attatttttg
     4081 ctataaccgc cttttgattt gtttccacga taattaccgc cgtcacgacg attacggttg
     4141 tttccgccac cacgtttgcc atttttattg tatccttttt tctgcattgg caatgggcgt
     4201 tctggtgtga tcttcactgg cactgcatca gctggatctt ttgctgtcgt ttttagcaaa
     4261 agtgcaacta aatcttgtgc agaatattct tctaataatt tatctgctgt ttgcaggtat
     4321 ttatctagac cattttctgc aagttttgtt tcgatttgtt caactgcagc acctaattga
     4381 cctttgaatg cttctttttc ggttggtgga cgtaaagtcg tcatccgttt tttcgtcaag
     4441 ttttcaatca catgcaagta gtccatttca tttggtgtaa cgaaagtcac agacataccg
     4501 ccttttccgg cacggcctgt acgtccgata cggtgaacgt agctttctgg atcttgaggg
     4561 atatcatagt tgtacacatg agtgacacct gaaatatcta atccacgtgc agcaacgtct
     4621 gtcgctacaa ggatatctaa atgaccgctc ttaaatgaac gcaatacgct cattcgtttt
     4681 tgttgagaca aatcgccatg gattccttct gcacggtaac cgcgtgcttc taatccgcgt
     4741 gccaattcgt ctacacggcg ttttgtacgt ccgaagacga ttgttaattc aggtgtttga
     4801 acatcaaaca agcgagtcat gatatcgaat ttttcgtatt cttttgcgcg gacatagtat
     4861 tgatcgatca agtcagctgt catttctttc gctttgatct tcacatgatg tggatttttc
     4921 atgaatttaa cgccaatatt cttgatagct ggcggcatcg ttgctgagaa aagtaatgtt
     4981 tgtctttgat caggaacttg tgaaatgatt ttttcgatat cttccaagaa gcccatattc
     5041 aacatttcat ctgcttcatc caaaactaat gtttgaacgg ttccaagttt caatgtgtgg
     5101 cggttaatgt ggtcaagcat acgtcctggt gtacccacaa cgatatgtgg acgatctttc
     5161 aagccacgga tctgacggcc aatatctgct ccgccgtaaa cggcttgtac acgtactttt
     5221 ttgtcgcgcc ctaaacgata taattcttct tgtgtttgga tagctaattc acgtgttggt
     5281 gcgataacta aaccttgtaa ttcgtgacga tctggatcga ttttttctaa cattggtaat
     5341 ccaaatgcag ctgttttccc tgtacctgtc tgtgcttgtc caataacgtc ttttccagct
     5401 aatgctagtg gaatcgtttc tgcttggata ggcgttgctt cttcaaaacc agcgcgttcg
     5461 actgatttca acaattctgg tgataattct agttctttaa atttcaaatg aatcctccta
     5521 tatggttgtt aactgaggat gtagcttttt tgctaccccg tcgatttgtt cggtcaaagt
     5581 gtattgtttc tttttgacag tggccacgca cgtttttatc cattcttagc ccctgtggca
     5641 ccaccgaaaa atcactttgc gaagtttagg cattagaaaa gccaaacggg tcgcgttcag
     5701 ctttatcatt attatgacat ctggcttcaa caagttgttt ctatggcaaa accagtccaa
     5761 tttctcttca tctttcggca ataggttatt ctaacatagt ttttcgtttt ctgcaagttt
     5821 acagattttt atttcatttg ctgaagttgt tctaccactt caaataagcc catgctgttg
     5881 ctgcctttta gcataatact atctgttgct tcaagatttt gtttgatagc atcaatcaat
     5941 ttattctttt ctttttcaaa atagaacaca ggtagttggg ggtatttttg gttcaattct
     6001 tcttttagtg catgcatttg ttccccataa aggaaaatca tggcgaattt ctcaggatcg
     6061 atatgttcac tcatttgcgc atgcatattt aaagactctt tccctaattc cagcatatct
     6121 gctaacacag cgattctgcg tccttttgtt ggcagatttt taaaagtatc taataccagt
     6181 cccatggctg ttggatttgc attgtagaca tcgctcaaaa tagctgcacc atttcctgcc
     6241 gttagccatt cggttctgtt ttgagtgata gaaacatggg cgagcccttg gcgtatctca
     6301 ggtatggaca gtccaaagaa gcgcccgatc gtatacgcaa tcaaagcatt cgttacatta
     6361 taacttccag gcagcgggat cgtaaatgtt tctccttcta ctacaaaatc ggtttgtgct
     6421 tttccttttg ctgtcacttc cgcatgaaca tcgccatttc caataccgaa tgttgtgact
     6481 gtttgtacta atgttgccgt taaaggtgtc agcaacggtt catctgcggg cacaacgagt
     6541 aatccgtctg atacaagtcc gtcagtgatt tccattttgg ctttagcgat tccttctcgc
     6601 gacccaagat tttctatgtg tgcttcaccg atcattgtga ttgctgcagc ttttggttga
     6661 gccataagtg aaagttccgt gatttcattg gcgtgatcca tgcccatttc caaaacgatc
     6721 ttttctgtat cttggggcat gtgcaaaatc gtgtaaggaa gaccaatatt attgttgtaa
     6781 tttccttgcg tcttatatgt tttgtatttt tcagacaaga cagacgcggt tagatctttc
     6841 gtggtggttt taccattgct tcctgtaatt gcaacgactt ctgcgcctac tttttccaag
     6901 taataggttg ccaattgctg catggctttc aaaggatctg ctacaggaat aactggaaaa
     6961 ttttttggag cttgttcaac aggcaagctc caaaatgaag cagatgctcc gtttgtgatc
     7021 gcttgctgga taaattcatg tccatctcgc gtaccagcaa gaggaacgaa taaacttcct
     7081 tcggttatct tccggctgtc aaattcgaca ttggtaatct ttgtcagtgc agagcattcg
     7141 ctgcccacgg cttgtgcaat ttcttgtatt gttagattca ttgagatctc tcctaaaaaa
     7201 ggttgtgaaa aaagcgtttg gcctaaacgg cgggttcgca tgactctcac ttccaagtaa
     7261 atttctctat tcgtatttag aagctagaat gaacgcggtt acgcaatcac tccagcctct
     7321 tctttatttc tctttattca tttttttcaa aaaaagattg acgctgatgg tatcgattca
     7381 ttcctaactg gatcagttct tcaatcaaat caccgtattt caagcccata ttttcccata
     7441 agagtgggta catactgaac tccgtaaatc ctggcataga gtttaattca ttcaggaata
     7501 attcattttt atttgtcaaa aagaaatcgc accggctcaa tccgcttcca cctaacatcg
     7561 tgtaagctaa cttcgcgtac tcttgcgctt tttgataaac ttcttctggc acttcggctg
     7621 gaatctgcat ttcgatttta ttattgatat attttgcttc ataatcatag aatgctacgt
     7681 cttttacgac ttcaccaggc aaagtcgtcc gaacgtcttc atttcctaat acagcaactt
     7741 cgatttcgcg cgcttcaatt ccttgttcaa cgattgctcg agaatcatac tgataggctg
     7801 ttgctaaagc attttgcagc tcttctcggt tttctgcttt tgtaatgccg acactagaac
     7861 ccatattcgc cggtttgaca aacatcggat aaagcaaaga accttcacat tgatcaaata
     7921 cttttttagg attttctttc cattgattct taagtactgg tacataagga acttgcggca
     7981 caccagcagc ttgtaaaata tacttggtca tgattttatc tgcactggtc aatacgcctg
     8041 cgccgacata aggcatattc aatgtctcta agaagccttg gatcgttcca tcttccccgt
     8101 ttggcccatg taaaactgga aaaacgatgg ctccttcttc tttgatttcg cccggattga
     8161 tcacttttcc tgtaaagcct tcctctgtct gtccacttgg gtcccatgaa agatgcaaga
     8221 catctttgct ggcaggtttt tctgttagta atggaccttt gacccattgt ccttcttttg
     8281 taataaatac gagttgaact tggtaataat tataataaat ggcatttaaa actgaaaatg
     8341 cggaaagaat ggacacttca tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca
     8401 aaagattgtc ctcctgtatt ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa
     8461 acaaaaataa acgaataact tatttataaa aaaacgagcc agcttctaaa agaacgagcg
     8521 tcatttattt tttgtcagct agattatttg tcccgttttt tataaaaagt tatgataaag
     8581 aaaaggaggg atgatgatgg cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg
     8641 agtgttcatc aattacaatg tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt
     8701 aaaaaaagcc tttgaagaac taggccccac cttcatcaag ataggacaga tgctttctgt
     8761 tcgagaagat ttgctttctt ctgccttcac tcaaacattc aaaacattac aagatagtgt
     8821 acctagcgac acattttcaa cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt
     8881 atcagatata ttcgatgatt tttcaaaatc gccttttgcc tctgcttcta tggggcaggc
     8941 tcatcgtgcc aagctgaaga acggtgacgc ggtagtcgtt aagatccaac accctaacat
     9001 cgccgaagag atccgacttg atcttcaact atttgaacga gcgatccctt tgatcaaata
     9061 cattcctgaa actagcgtag ttgatctaaa aggggtactc caagaagtaa aacgttcctt
     9121 gatcaatgaa atggacttct taaaagaaag tcaaaatggc gaacaattct atcaaaagaa
     9181 caatggctgg aaagagatcc gcagccccaa gatttatgac gctttttgtt cgaaaaaagt
     9241 gatcgtgatg gaagaaatgt ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa
     9301 aactgaaact tttatcactg gaattcaaaa caagcagctg aaacaagaag ttgcaaagct
     9361 tttagttgag aactttatga aacaggtgtt tgatgatggt tttttccatg ctgatcctca
     9421 tccagggaat ctgctattcc atgtactaac aaaagaagaa cagacacaag ctagccgaaa
     9481 aactgaaaca gtccatgaaa aagagttcgg ttcctttgct tttcgagcct ccacttctgc
     9541 agaagaccct gttgcgccat ataccatcaa ctacatcgat tttggtatga tggggcatct
     9601 ttctgcagga cttaggcaaa aactcacaca agccgtttta gcgctgtata cgaaagatgc
     9661 ttatcgaatc gaaaaagcgg tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag
     9721 tcggttccac caagaactga caagttttct tgaacagtat tatgattctc ctattgatga
     9781 aatcaacttg caagaagtat tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca
     9841 gttcgatcgt gatatcacgc ttttgctgaa agcatttggg acattagaag gggtgatccg
     9901 cgtcctcgat cctgaagttt ccctcatgga agtcgcttcg ccttttgctc aacattattt
     9961 tctcactcat ctggatgtag aagatacatt gaaacaaagc ggattagact tattggaagg
    10021 gatgaaggct gcgcctaaga tccctcaaca gctccaccat ctattagaaa tgtggacttc
    10081 gggacaagga aaagtcaatt tggaattgaa aaagcaagac aaattattga gcaggatcga
    10141 aagtatgatc aatcggcttg ttttcggtat gattttagct gcattgatcg tagggtcctc
    10201 cctgcttgtc caagcagcac ctgtagaaaa cgcagaggtc gtttccttat tagggatatt
    10261 tacgtatgct attgccgcat tcgtcattat ttttttggca atcgatgcat tgatccaaat
    10321 gtataagaag aggaaaaagt agaaaaaggt tgtgacagaa agaatcagct tcaagaaata
    10381 agaaggggtt ttacataaat tgtttctcaa atttttgtga aaattcggct tatttttcga
    10441 agaggctact tctgaaacac cgagaataaa aaatgtgaaa aagctgttct gcacccagta
    10501 ataagaacaa tcaaaaaggg aacatggaac acatttatcc tgtttccctg ttcccttttt
    10561 gatgtattcg agcataaata aaataccttc gttacctact atcttctgta cttggtaaac
    10621 cttagtcaat tgcctttttt aacgcttgac gaaaagacat atgatctttc tccgtaaagg
    10681 gttttggacc ctttgtcctt atgccagctt ttcttaactt tccactatga taacgttgtt
    10741 ctagaagcgt gccgatattc gcttcgtcaa attcttttcc agagtgatgg aacactcgca
    10801 cacccttcgg caatacaagt gaagctaaac cataatcttg cgtgatcaaa aagtcattct
    10861 tcttaaccat accgacgatt ttataatccg cactatcagc tccgcggtct acgtagacga
    10921 tttggacatg attagcgtat tctttggtcg tataatggtc cacactggtc acgataacca
    10981 cgtcaagacc ataggattct cctaatttta tgacatcttc ttttactgga gaaccatctc
    11041 catcaatgaa tagcctcatt ctttttcgcc ttcttccata aatgataaac agttactaat
    11101 ccgctcagta aaagaccaac attgatccat tttgccaaga taagcagttg tacactttct
    11161 atcggcactg attcatggtt cagcgtgatc aataatagat ggattggctc tcccatttct
    11221 tttgctaaaa caaacaaaag cgtcatcaat aaaaactggt ggatcacact ccctttattt
    11281 tgagtagtat acttgacaac gatccggcga tccattggaa tagctgtatc agtttgataa
    11341 tattccttat ttagccagct gcctgttact acgtgccaga aactaagcag aaagacacct
    11401 atcgcaaaac catttggaat caagaggacc aataaagaag aactcagtcc aagcaattgc
    11461 atagccaatg gagaccatgt taaacgtttc aaaatccatg gtccgactat ttttgctgca
    11521 atcaagcctg ctgcataagg caaatacata aaaatcgaaa gtttttctaa tccgtacagt
    11581 atgccaacat aaaaactgct aaataaaaaa aggaaattcc ctagcattcc atttagaaaa
    11641 gtaaacagat ttagccagct tggcaacttc cattccttct tctggtgtcc taaaacaaac
    11701 acgaacaaga tgaacagcag caagaaacca taaatagcaa gatcaaaaag cctttcatcg
    11761 aataacaatc gggcatttct caaaagaaac aataagccaa agaatatcaa aaataaaaca
    11821 agctctcgcg tagctaccag atttcttttg atgtctttga aatccagttc gtaacgcgga
    11881 taatgactaa ccgtatggta ggccatgacg taaaaaagtg tataaatagt aaagcttgct
    11941 gcaatctgag tcggtgcagg caactcaatc gcttgataca ggagaaatag ccagatagct
    12001 gcaaataagt actttttccc atccatattg ataaaacctt gcgtcttttc gtgaaaattg
    12061 accgtggtat tagcaggtac aagccatgct gcactcagcc ctagcaaaac acttgaaaaa
    12121 taaaataaag gaaaataaaa tacgcctaat attccaagca atgaaccagc accaccaatc
    12181 agcagagcga tcatcagcaa cgtgtaacta tctagaccaa atcgtagact gttgactaaa
    12241 aatagtccag tcatccgaaa ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta
    12301 tgcgagtctg ttagtcccaa aaataaaaga taaggaataa aaataaccgt gttacttaag
    12361 ataaagggcg ttgtttgtgt aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca
    12421 atggctcctt aaacagtctt ataactaatc tgctcttcga ttttagcatt aaaggggaaa
    12481 taaaccaatt ttacagaata tattcttgta aaaaaaaata cagaaagaag cataagcttc
    12541 tttctgcatt tttacggtac ttgattactc aagcagacaa gcccttctcc acaaaagtct
    12601 tgcccgacac ccctgcatcc ggcaggtgat aaagatagaa atcaattatc ctgtgcctcc
    12661 aaaatcaatt aggagctctg taaaccagag tccttctcga cccgtcgcat aaatttactt
    12721 taacacagtt cctatgttta tgcgagagct tttttcattt tataatcgac catttgaaat
    12781 ccttgaccga tatctgaaat aagttcttct gtaatctcaa atccttcatg cttataaacc
    12841 tcgattgctt gatgattccc ctgattcact cgcaaaaaga tctctctttc ctttgtttgc
    12901 gcagcaagtt cttcatacca gcgaaaaact gaagaagtca atccttgacc acgtacttta
    12961 tttagcaaat ataatttact taaataaatt tttccattta tctcttcata agcagtgtaa
    13021 ccgacagtct tttcttcgct ttttaataaa aagtaggaca caccttctgt cagttctttt
    13081 tgaatctgtt tttttgactg ataagttttc agcatgtaat ccacttgttt ttgaccaatg
    13141 atcggcacaa aaacttccgg ccatacttct tgtatgactg ccatcagctc atccagttca
    13201 ttttctgtta cttttatttt ttccatacac ccacgtcctt ttttacatta taaatgacct
    13261 tgcgcttgat ggaataaaaa gagcatgatc atgctataca cacccaccga ccacggtttc
    13321 aagaaaatca caatctgcat aaattcccta aaagataatt tcgtcaaacc agctactaga
    13381 cagatcaagt cgtctggcgc aaagggaagc agcaaggtaa caactagtac ttttttaata
    13441 tctttcgttt ttcttgtcag caattcatca aatttctgat acttttttgg cgataaaata
    13501 agctggacaa aggctacgcc atagtaacgg acaagcaaaa atccgattgc ttccccaatc
    13561 actaggccaa tacaactata taaaagccca aatccattgc caaacatcag catgcccgct
    13621 actgaggaga tccctcccgg caaaatagga atgatcactt ggatcacttg caacagaaca
    13681 aatatcatca ctgcatactt gccaaattgc tggataaacg cttgcaaaga cgtgctcgat
    13741 cgaaaaatcc catgatgata agcataaaga atcaacacaa agaaaaaaaa cagaccaata
    13801 atgggcatga tctgaagaca tttttgtaaa actgatcctt ttttagacat ctatattccc
    13861 cacatctttt ttcatttatt aaaactgcct cct
//
LOCUS       DAZKBH010000069        13810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000069 DAZKBH010000000
VERSION     DAZKBH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.069"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACG8OO_001925"
     CDS             complement(221..1423)
                     /locus_tag="ACG8OO_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9045792.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACG8OO_001926"
     CDS             complement(1426..1620)
                     /locus_tag="ACG8OO_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9045793.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACG8OO_001927"
     CDS             complement(1667..2515)
                     /locus_tag="ACG8OO_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFP9045794.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACG8OO_001928"
     CDS             complement(2720..3070)
                     /locus_tag="ACG8OO_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045795.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACG8OO_001929"
     CDS             3248..4111
                     /locus_tag="ACG8OO_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9045796.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACG8OO_001930"
     CDS             4735..5727
                     /locus_tag="ACG8OO_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9045797.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACG8OO_001931"
     CDS             5743..6627
                     /locus_tag="ACG8OO_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9045798.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACG8OO_001932"
     CDS             6676..8283
                     /locus_tag="ACG8OO_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9045799.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACG8OO_001933"
     CDS             8284..9768
                     /locus_tag="ACG8OO_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFP9045800.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACG8OO_001934"
     CDS             9945..10937
                     /locus_tag="ACG8OO_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9045801.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACG8OO_001935"
     CDS             10989..11858
                     /locus_tag="ACG8OO_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9045802.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG8OO_001936"
     CDS             complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG8OO_001936"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9045803.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAZKBH010000070        13782 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000070 DAZKBH010000000
VERSION     DAZKBH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.070"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..246)
                     /locus_tag="ACG8OO_001937"
     CDS             complement(<1..246)
                     /locus_tag="ACG8OO_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9045804.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFL"
     gene            complement(725..1444)
                     /locus_tag="ACG8OO_001938"
     CDS             complement(725..1444)
                     /locus_tag="ACG8OO_001938"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9045805.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1493..3139)
                     /locus_tag="ACG8OO_001939"
     CDS             complement(1493..3139)
                     /locus_tag="ACG8OO_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFP9045806.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3318..3908
                     /locus_tag="ACG8OO_001940"
     CDS             3318..3908
                     /locus_tag="ACG8OO_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9045807.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            4000..5505
                     /locus_tag="ACG8OO_001941"
     CDS             4000..5505
                     /locus_tag="ACG8OO_001941"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFP9045808.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5589..6941)
                     /gene="celB"
                     /locus_tag="ACG8OO_001942"
     CDS             complement(5589..6941)
                     /gene="celB"
                     /locus_tag="ACG8OO_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9045809.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6941..7459)
                     /locus_tag="ACG8OO_001943"
     CDS             complement(6941..7459)
                     /locus_tag="ACG8OO_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045810.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7475..7801)
                     /locus_tag="ACG8OO_001944"
     CDS             complement(7475..7801)
                     /locus_tag="ACG8OO_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFP9045811.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7814..9793)
                     /locus_tag="ACG8OO_001945"
     CDS             complement(7814..9793)
                     /locus_tag="ACG8OO_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9045812.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9814..10128)
                     /locus_tag="ACG8OO_001946"
     CDS             complement(9814..10128)
                     /locus_tag="ACG8OO_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFP9045813.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10296..10589
                     /locus_tag="ACG8OO_001947"
     CDS             10296..10589
                     /locus_tag="ACG8OO_001947"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFP9045814.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10667..11779)
                     /locus_tag="ACG8OO_001948"
     CDS             complement(10667..11779)
                     /locus_tag="ACG8OO_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9045815.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11932..12780
                     /locus_tag="ACG8OO_001949"
                     /pseudo
     CDS             11932..12780
                     /locus_tag="ACG8OO_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13133..>13782)
                     /locus_tag="ACG8OO_001950"
     CDS             complement(13133..>13782)
                     /locus_tag="ACG8OO_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFP9045816.1"
                     /translation="LQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYEL
                     DATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVL
                     QSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4326 a         2799 c         2355 g         4302 t
ORIGIN      
        1 gagaaatgct tgatccgaag aaaataaaaa tttgaaattt tcccaaccca cccaaggact
       61 ttccctcaag ctagccaaga acccatctgc tgaaatatga aaatttttga atgctacgac
      121 attcgccatt acaggtatgt aaaagaaaaa aatcatccaa agcatacccg gtacagccat
      181 caggacaagt gctttataac gccatagatt cataaaaaaa tgattgaacc cctttcgttt
      241 tttcatctaa aaaccccttt cgattcattc gcaacgtaat tatagcgctt tcaataatga
      301 gaataaataa acaaagtata gggaaaatga aacaaagtat aggagaggtt gtgaaatcag
      361 cgttttggcc tgagtattag agaaaaaaac gggaaaatag ttcctcatat tttgccgttt
      421 tttgagctaa taccgagggt cagctgattg aacaccgttt atcagaggtt gtgaattcag
      481 cgttttggcc tgagtattag ggagatcgtg tccttactat ctaactctga gtcacaagga
      541 acaatgggga ctgtaacaaa agtcttgtca cagtccctta ttccgaacaa acggtgttcc
      601 agaagtagct tctacggaaa taagccgaaa tttcacaaaa aagccatccg aaagtcagac
      661 ggacaagtct gcttttcaga tggcgctaca actactctgc ttattctttt agctatccat
      721 tcaattattt ttctctcact tgttctagct cttctttggt catgggtcga tattgccctt
      781 tattcaaatc tttaggcagc caaaattttc ccatacggat tctttttaaa tataagactt
      841 cttttcctac tgctttgacc attcgcttaa cttggtgaaa ctttccttca tgcaaaatca
      901 gacggataat tgaagtttct gtctcttgat ctacttgatc aatttggagt tttgccggca
      961 atgtctgctc atcgccatca ataatcagtc cttctgcaaa taaacggata tcttcttctg
     1021 tcattatacc gctaacctca gctagatatt ctttctccac atgtttttta ggtgatagta
     1081 actgatgggc aaacaccccg tcattcgtta aaatcaagag gccctctgtg tctttatcta
     1141 atcggccgac tggaaaaaga tcttcccgat aatcctggtc ttccaataaa tcgattacgg
     1201 tttgatcatg attatcctga gtagcagaaa tgactcctgc aggtttatgc aaaatgtagt
     1261 aaaaatcttt ttgatacgta atgatttctc ctaaataagt aaccgcatct tcgaattcat
     1321 ttacttgcgc tttatcgctt ttcaccactt gtccattgac ttcgatcgtt ccttttttga
     1381 ttaatccttt gacttctttg cggctgccaa gtcccatatc tgctaaaaac ttatctaaac
     1441 gcatgcgttt cccccatttc aaaagagcat caatctagct cttctgattc acttatttta
     1501 tttttaattt atgtcgtaaa cgcgctattc ctggtcctag tagtctttct gccaagcgga
     1561 ttttcaaagc taaataacca tacactaaag cccctatacc tgctactaca agaatcaaca
     1621 atagagactg gaatttgcta tcttcgttta aaaaagcacc aaaaatcatc tttgtgatcc
     1681 acgaagccaa aagcataatc aaggtaagaa taaagatcag caatgtcctg cgccagacaa
     1741 aacttggttt aaagcgggct acttcatgga ttctctttaa gatcaaggta caagacacag
     1801 caaatccgat cattgtcgcc aataaagggc cgtatacttc aaataaacga atggctgggt
     1861 attgaagaag gagtttcact agaaacccaa taaccaaata attaattgcc gccttgtttt
     1921 caaacattcc ttgcagcatg ttggaaacta gcatatatag tcctaggaag agtcctacaa
     1981 aacttgcttg gatcagtaca ttacttccta gagaatccgg tgtataaaat aatgtattta
     2041 gcggataggc aagtaacata acaccaaatg tcgccggaaa cataaaaaat gaaaacaact
     2101 gtaaatttcc gcttgtcaat tttgccagac ctactcgatc tttcaacgta acagcttctg
     2161 taatcaacgg taacccagta gacgcgatcg atgttgccaa tgcaataact accatcgtca
     2221 acttatctgg attggcacta aaaatcgaaa aaagatccag taactgtgca ttggaatact
     2281 cggtggatgc agacatgatc ttcataaaag tgaactgatc gactaatttg aagatcgtta
     2341 cccctgaacc cacaataata aacggaatcg cttctttaac ggtatctaac aacaattcct
     2401 tcgttgcaat atttattttg ttttcgctgt agtgaataaa cgccgatgta tacgcttgat
     2461 gtttcttcaa aaaatacaaa aggacaagaa tacttgctag cataccgata aatgccgcga
     2521 aagttgattg cgtgaccgcc gtcacatagt caccatccat gactttcatg atgatgaatg
     2581 tagccaatag catataaaaa atacgtgcca cctgttctac aatctgcgac agcgcataag
     2641 gcatcatttc ttgattgcct tgcaaatacc ctcgtatcac actcatacaa ggaaaaacca
     2701 atacagcgat actcaaagaa cgcatcgttg gaatcagttc ttctccgcca ccagaagcat
     2761 gagctagcca aggagaggcg atgtacatga acaaagcaaa caacaggcct aacccgccca
     2821 tcatctgtaa tgcacggata aataatcgtc gtgaagtgcc atattcattc aatgaattat
     2881 agcgtgcagt ttgttttgca atcgcagcag gtattcctgc tgtagagatc agtagaaaca
     2941 atgcatatat attgtatccc atattgaata atccattggc agccttagca ttttcgccca
     3001 tccaagcata ccagggaatg atatagacag cgcctagcaa tcttgaaatg atattactgg
     3061 cggtcatcca agcagagcct cgtgccattt tttcttgcgg tgttaattga tctaatgctg
     3121 gttttctttg attatccatc ggtcagattc caactttcta caaaacatta ctatcatttt
     3181 aattggtaaa acagaaactg gcaatcgatt ttatataact tttataagaa caattgtttt
     3241 tttgaattcg gtttcgtttt tttggtaaag taaagaaata tacagccttt tatggctaac
     3301 atttatagga gatatcaatg acacaagaaa taaaaaaact acccgttata tttttaggtc
     3361 ttttctttat cacagttgga ttgaattggt tcttgctccc tcacgatata gcttccgccg
     3421 gagtgggcgc catcggacat ttagttgaaa cggccttttc tatccaacgc tcagttactg
     3481 tttggagcat caatcttatg atgctgcttc tagccgctgt cctattagga aaatcagtat
     3541 tttttaagac tgtagcggga agtctgcttt ttcctgttgt tttagagatt gttccaaaag
     3601 tggaaatgat ctcctcacat ttcttttcac tgatttcagg aagtcttttg ttcagtcttg
     3661 gggtttacac attgtacaca gtcggggcct caaatggcgg tataacgata ccgccgatta
     3721 ttttccacaa attttttcgg cttccaatgc cgcaaggact tttacttacc aatagtctta
     3781 tcgtcttttt gaattattat gtttttggat tgttggagac actattcgtt ctcctatcta
     3841 ttacagtcag ttcattgttt atgaaaatac tcatccgctt gtcccctgtg tcaagaaaat
     3901 ttgaataaag ctggattttg tcatagcgga aacaaacgat atattaaaaa gagtgtgcta
     3961 taataaacca agaaattgaa taaagcgagg gatcaatcaa tgagcttctc tttagaagac
     4021 atccgtcatt tactattaaa agaaaatcta ttaaaagaat ttgtttcaac aagaggctgg
     4081 catcttgatg taccagaaaa agctgaattt cttcagttaa gttatgattc aagaaaagct
     4141 gatgcttcta ctttgttttt ctgtaaagga atgaatttca aagaagaata tttggattca
     4201 gcaatcgaac agggtattca atattatgta tcagaacaac catacgaaaa ttcagcagta
     4261 ggaatcatcg taacggatat ccgtaaagca atggcgcttc ttgctatggc tttttacgac
     4321 tatccgcaaa ataaattgaa agtgatcgga ttcactggaa caaaaggtaa aacaactgct
     4381 gcgtacttta caaaagcaat cttggatcac acaacaaata aaaaaacagc attgctttct
     4441 actatgaata caacgttaga tggcaaaaca tatttcaaat cccatttgac aacgcctgag
     4501 tcattagatt tatatcgtat gatgtcagaa gcagttgaaa atggtatgac tcatctagtg
     4561 atggaagttt cttcacaagc ttacaaaaca caacgcgtat acggcttgac tttagatgtc
     4621 ggaatcttct tgaatatctc gcctgaccat atcagtccga tcgaacatcc gacatttgat
     4681 gactatttct attgcaagcg acaattgatc ttgaattcaa aaacagttgt tttaaatcat
     4741 gaaagtgact attttgattt acttagggaa acagccgatc tattccagat tccaacgatc
     4801 acttatggtc gttcggaaga aagcaattat caagtgatcc gttcagataa aggtactcac
     4861 ggttttactt tatcttctca tgaagatcgc ttagctatct gcgatactgc ttatgatatt
     4921 ttgctggctg gaggattcaa ccaagaaaat gcagccagtg cgatcattgc cgcagcacta
     4981 acaggtgcct ccagtcaaga tgcacaagac ggattaaaag aagctcgtgt accaggccga
     5041 atggatcaat tgatccaatc caatggagcg catgtctatg tggactatgc acataattat
     5101 ctaagcttga aaacattgct gaaatttgcc aaaaatgaac atccagatgg ccgtgtaatt
     5161 gttgtattag gcagcccagg aaacaaagca atctcccgtc gacatgattt tggaaaagtg
     5221 ctatcagaaa cagctgacat cgcctttttg acggcagatg acccagcatt tgaagatccg
     5281 aaaaaaatcg cggaagaaat caatgaagca attactaatc cagatttgat cgttcattat
     5341 gaaatggatc gtcctgaagc gattcgacgc gctttagcag aaagcgctcc gcaagattca
     5401 gtcgttattg cgggaaaagg cgtggatccc tatcaaaaaa tcaatggtgt agatgaacct
     5461 tatgaagggg attatgcaat cgtgaagcgg ttgattgaag aataatggta tttatatact
     5521 agtggtttcc catttctaag aaaaaaaggg actgtgattt tttgtcacag ttcctttttt
     5581 cttagaaatc attttaactt ttcactagct aattctgctg ccttttctct ttcttcattt
     5641 aataatttat tatcatagaa tttgataaat gggaaccaga taatgaatgc gatgattgca
     5701 caaatgactg ctaacacagc agctttccaa tcaccaccac taccaacaaa tgctccgatc
     5761 ccgactggtg aaggccacgg catctgtgca agcatcggtt tgacgatttc cagccgaatg
     5821 gcaaaataag cgagtgtagc agaagccatc ggagctaaga aaaacggaac cgctaaatag
     5881 ggattgtaga cgattggcat cccgaaaatg attggttcat tgatattgaa taatcctggt
     5941 acaagtgagg cttttcctaa cactttcagc tgatcagatt tcgcgaggaa tgcaataaag
     6001 atgatcaatc ctaaagtcgc acctgatcca ccaacggtta cataagaatt gttgaattct
     6061 cctgccaacg gataatttgc gccttgtgcg ttagctgcca tattagctaa tacgatcggg
     6121 gtcaagaaag aagtgatgat attcgctcca tggatcccta cgatccacaa tgcatggatc
     6181 aagaaataaa tcaccatgac accaagccat gtattcgtca actgcgtaac aaacccaaat
     6241 gggatagcaa tcattttgaa gatatccgtt cctagtgcaa ctaataatcc attgatgatc
     6301 atcaccacaa acgcaatgac aaatgttggg atcagagcag tgaaagaacg agaaactcct
     6361 ggcggaacaa catctggcat ttttatgacc caatttctct tgacacacat acaatacaat
     6421 tcgaccgcaa taatcgacat aataatagct gtaaagattc ctgaagttcc taaacgttcg
     6481 acaaaagagc ccatcctaat accatttact acagtttctg tctcattgat agaagcaatc
     6541 aagctgattt taccatcagt catgatcaat tcagggatac acataaagaa tgcaaaaaca
     6601 gatagtaatg cccccgtgag cggattaaca tttaattttt gttcatctcg ttcaattgac
     6661 gttaattcat aagcaaacac aatggcaaag taaatagata aaatccccat cgtcattgta
     6721 ttgacgatca tgtacaagtc actaactttg aaaaaagttg cattaaaaaa tccttctaaa
     6781 aatgggaacg gtaatggcaa aatatttaaa accaaaaaca tcgagcctac aatcgtgaaa
     6841 ggaatagaag ccatccccgc tgccatgaca gcccgaacga ttttaagttg cgcaattttc
     6901 cccataggtc ccatcaggta tttctgcaaa aaatcaaaca ttagttattc tcctttccta
     6961 aatataacgc tttttcatat tgtttaatac ggatatactg ttggtcttgg ttgagtgaaa
     7021 ttttctttaa acgatactgt ataaatgcac tggcaaaaat accgcttaca acaagaatga
     7081 aaacaaataa ctgtcctcca ttgttatttg ccataaaagg atacaagaat gtaaatcctg
     7141 aggtaaacaa cactggaatc actaataaat taaagacact ctggaacaaa aaataccact
     7201 ttgtccatgg aacgttcgtt tggtgttttc gataaagctt tacttgttct cctactgcag
     7261 gcaaaatagc taaaatcaga atcatcggta aactacttcc taaactttta gctgatagaa
     7321 gcagaatcat ccattgcatg ttcatgaaaa agaaaaaggc agtcaggtaa cgtacgatta
     7381 gataacgatt gaaatacatg tttttcaaac taagttgctt tttttctaat tcgattttcc
     7441 tttttactgt atcctgcaca atgatccttc ctttctattt atttttttga tacaaataat
     7501 tcatttcctg tgcaacttcc aataatgtca tcgtcgtcat cagatgatct tgtgcatgga
     7561 ccatgataat ttccatttct atttttgtcc cgctggcata ttctttcaga agttgagttt
     7621 gtgactgatg cgcatgaaga atctcttcat ttgcttgtcg caacttctct tctacttctt
     7681 caaacatctc tttacgcatc gagcgaaatg cttcatgtat cagtgtccgt gcatttccgc
     7741 tatggaaaat gatatcaaaa gaagtcactt gtagtgtttc actgtttatt ttttcagcca
     7801 tctgttttcc tccttattcg atcagtttga taaatttact gctgaaattc tcaaaaatag
     7861 gttcagctaa gatttcctgc tgtactttta gctggtcaac taacctaacg attgctttag
     7921 tcacgaccgt aattccttca ttttcgacat aagaaggcga aactaaaaaa acaaaatgaa
     7981 tttcttgata ttgatcccac tgcatcccag aaggaatcaa tgcaacggct acctttgttt
     8041 cgattccaac tggaacagcg ggatgtggga ctgccactgt atcactgaat atgatttgtc
     8101 ccatattttc acgttgctgg atctgctttc gcatttctct tatatatcct tcttcttcat
     8161 ttatgctcag cttttgcagc aaatcatgaa gaatttcttc ctttttcggt tcgtacgtat
     8221 aagtcttgaa aaatttttca gacaactgtt cggtgtaaat ctgctttttt tgctgtagag
     8281 aaaatgctgg tatcggtcta tctgaggaat gatagggtat agattctcca actacctttc
     8341 gtattttacg aacatcatct tcattcaaaa aaatactgac atgtaaaact ggaattttga
     8401 aaaacattgt cgaaagatca atagaagaaa taatcaggtc gattcctttt aaagttgttt
     8461 cattgatctc ataatagcct tttacactta ccactgtaat atttttaccg aattcactga
     8521 ctacgcgatt ctttaatagt tgggcactgc cgtaacccgt tgcacagata atcaatgcat
     8581 gtagtttatg cgcagctctt tctttttcca atgccgccat gagatgcaaa gccaaatagg
     8641 cccattcatc ttcattgatg gtatagtttt tcaaagccgg catgttgctg aaatgccttt
     8701 ttactgcgtc aaagactttg ggattttctt tctttatctc ctcagtcaat gggttctcaa
     8761 tgcgaatacc tcggctcaat cgaatcatca tcggtgacaa atgatcgagt agtccattga
     8821 aaagctggag atcctctact aatgaaaaat gaaattcctg agacaatctt ttcagaactt
     8881 tcgctaattc ctttgatagt tcctgatcat tttgattttc tttgtgattc gacttagcca
     8941 tgagatgtaa tgtcaaataa gcaatttctt cttcgggaaa cgatacacct gcgattacct
     9001 caatacgttg aacgatccgc tgcgccactt gatactctac tcgcttagaa acttcttgtt
     9061 cgatgcctaa ttccttgatt tcaaaccctt ctcggagccg tttgatcccc aaagccaagt
     9121 gtaaagtcag attctgtatg ataaaatccg aaacttttaa cttagcttct cttgtttcat
     9181 cgagaatgat aatgactagc tcttcaaaac tgatgtctgg gaaaaaacgg ctttgcccta
     9241 aatattcttt caatgaattt ccataatggt ttccaaaaaa agtatccatg ataaaatgcc
     9301 gtcgatttct ttcttcccct tcgatccatg tcccttgacc atgcttagaa acgattttta
     9361 gtgaatatgg aatcaatcgc tccttgattt cttgcatgtc ctttgtcaaa ctggaacgac
     9421 tgatataaag ctcttctgcc aaatcatcca taaaaagaat ggcatcttcc aaaagcaatt
     9481 ggttcaatat atacttttgt cgatcttcgg actcataaaa tccttgcggt tctctagcat
     9541 atccaggtac tatttcttgc cgactcaaaa acaagtcaaa tgtcagtctt tgtaaaatat
     9601 gcagttggta gccataacct ggtttagcaa tgatcttacc tccgtttttt tctactaact
     9661 ctttgatccg ctgcaagtaa ttacggattg tccgatcaga taataacaat tcagaagcta
     9721 aacgttgact ggtgacatac tccttcttgt gatcaatcaa atatctcaac agcaattttt
     9781 ccttctgttt cattatcatc cactcctatt ccatcatatg ttctcttcga tcacctctgc
     9841 taacttctgg atgcctgtag ggattgggac atacgcttgg aaagggatac taacaacagg
     9901 ctttccaaca gattctccta attttttgaa tttatccaaa aacatcgtcg tttgcgggct
     9961 gatcagaaat aaatcaaaat cactattctt aatcatatta tcgccttctg tagctgaaac
    10021 tgcatctacg agtacctctt ctcttttttg cttaaagaaa tccgttgtct ttgaggccat
    10081 catcgaggat gacattcctg ccgcacaaat aatcaatgct tttttcataa atcaatcact
    10141 ccttttattt ttataaacca agcatatctt gtaagcgttt tctattcaaa atatatattt
    10201 ccgtagcatt gcggaaaaaa tgtgtatcat atcttgaata ataatttctg gcaccgtgat
    10261 atactagtga tagaattttt tcaggagggg atttagtgag tatcacagtg aatattttgt
    10321 atacaagtac aaatggacaa gccagaaaat ttgcagaaga aatggtcgaa agaaacattg
    10381 tcaaagcgat tcgtgcagaa gaaggaaacg agaaatatga ctattacttt ccactagaag
    10441 atccagaatc tcttttactg atcgaccgtt ggaaagatga agaagcaatc gaaaagcatc
    10501 acaaatccga aatgatggct caaataaacg aactccgtaa aaagtataaa ttaaaaatga
    10561 aagtcgaacg tttcactgat ttaccgtaaa aagatacaca tggaatgata gcaatttatc
    10621 atccatgtgt atcttcactt tttccttatt ttttattcga tctcatctaa tgattcaaac
    10681 cgtgtttttc tcttattttt cggataagta tttcagtcac cccaatatac atgattccta
    10741 aacatacccc aaggatattc tcaaaaatgc ggatcgttac tgctcctgga accccgaata
    10801 aactggcagt gattgccaaa gcaccaaaag aattaaaaac tgttttatat ctataggtag
    10861 aacaaacacc taaagctaga ccacctaata taccgaccca gttaaaagga ataaattggc
    10921 tgataccaat aaataaaaca catccaatca atgagccaat gattcggtct actgctcgtt
    10981 ctttgaaacc aatcgacatc aatccatagc tagatacaat agaagaaaat gcaaaccctg
    11041 cccacatcaa tcgctgaaaa gggacatact ctcctgcaac gatcaaaaga ctaatgccaa
    11101 atgcatagat gagcatccaa attttttcct gcgaatacat gcctttcaaa aaaatctctt
    11161 taaacaagct tttgtctttg ttcttttctc gatgttttct gtaaaggaga actgaaaacc
    11221 aacaaaaaaa caaaaataat aagctacttg tttgtgtaaa gaaattcttg ttcaacgaat
    11281 ctttaggtaa cgaataaaca ataaagagat atgaaaagcc gtacaaagaa gcattcccca
    11341 ttttgggatc actagcggtt gccattaata tgctgcttag taaaataaaa tgaatcaaca
    11401 tgcccataac tgaccatttg atcagctgga catacggtgc tactaatagt gagaacatga
    11461 cgattgctaa acctatgatc gaatgggaaa tcttataacc aaagtcaaca aaacgaatac
    11521 tcatcaacat acagaataaa accacagcgt atggtttatg cttcgattca aataatgttg
    11581 taattataat gataaaacaa atggcaaaca atacgatgaa taatgatcgt aaaaacaatg
    11641 aacaaatata catgcacttt tcttttttag aagctgcttc tcgtattttt tgttttaaaa
    11701 taaacggatc tagttgtaga gactgataaa aatccacctg ttgtcgtcct ttctttttac
    11761 ttttattttt tacagtcatc tatgacaggt tgtaaatact atggaacatc atatggcgtt
    11821 gtcattttct tgtcaactat ttttcctatt catgttttta taaaaaaaca tatcgttttc
    11881 ttgtatgatt aaatcaccac aaaataaaca actgagagaa cgatatgctt tatgaatggt
    11941 tctatcaaaa aaattctgag atcaatagac aaaaatttga tgattacaga ggtctcttac
    12001 gaaaccttac agaagaaaaa aacattgatc gttgctgcag ttctcttccc tgctccatgt
    12061 gtctgtagaa attgtggttc tgctgtggta gatggaaata ggaaattaat ggtggtgaaa
    12121 aacaagaaga aggaaaccat tgtccacttt gaataatata atcatatgcc aatggttata
    12181 cgcctaaaaa agcagcgcta tacctgtaaa aactgtcgaa gccattggaa cgctcaaagt
    12241 tattttatcc gtccaaggca ctcgatttca aatcatgtca gacataaaat cacttcttta
    12301 ctgactgaaa aagtgtcgtt attttttatt tcgaaaagtt gttaggtatc tttgacaacc
    12361 gttattcgca cattgaaaga gttgagaagc tatttaccaa agtcttccaa aagaattctg
    12421 tcttgagtgt tgagggttgt gaatttcgtt ctcacgtttc cgttgaggat aaactgagtt
    12481 ttatttccgc ggatggcgag acagaaaaat taatcgagaa aatttaccta cacaaaaaat
    12541 tcctcgatta acaaactatt tccttagctg cactaatcct aaagaaatag aattcttagt
    12601 tacagacatg aacgccacct actttcagct caccaaacat gttctaccaa atgcgaaatt
    12661 ggtgattgat cgattacttg gtttttccac gcctttcaag gaggcgtaac ctttattcac
    12721 gagttggttg aagctttccg agacaaaaga cccgacttgt ttttctcctt attgacaaag
    12781 agccattaaa actagagaaa aaattacaac tgatcgctga ttatttgtag aaagaaaatc
    12841 aaagtattac ccaataaact tgagtcatta ggcaaaaaac actgcgaaac tcaagaatga
    12901 ttttcgcagt gtttttgtat attttttatt aactagtatt actacaaagg tttatttttc
    12961 acttctcaac gcagcacaaa attgtcttta cttatttact ggttgggttc aaccttctct
    13021 actccgatac taatatttgt ttgtataccc cttctaaaat cggtagtttc cattcaattg
    13081 atgggtacta gttgatttta aaaactatgc ttattcaatt ttttatttta gctcatatat
    13141 ttcgtttctt tctaaatttt atactgatga aaacaagtag cacacccaat attgacaata
    13201 taaactggct aatgatggtt tctccagttt ttggaagatg cttatttcca ctgcttgttg
    13261 gattgtttcc tcttatatct gagctatcag caacttttct attctctttt gttaaaaaga
    13321 tcgccacttc ttgttctttt tcgatcttga aagttactgg tgtcgcatcc aattcatacc
    13381 ccgttggcgc ttgagtttct accagctgat atgctcctgg tgctaagcca tctatcgcca
    13441 gttttccatc tgctcctgtg gttagacctg actgcagaac ggttccattg gcatcttgta
    13501 actcaaaaac agctccttgc aggacttctc cgctttggtc atcgatcttt gtcaacacga
    13561 caccgcctgg tgtaagtcga ttttctttcg tcaattctac caccgctgtc tgacttcttt
    13621 cgatctcaaa ctcgattggt gtcgcatcca attcataccc cgttggcgct tgggtttcta
    13681 ccagctgata cactcctggt gctaagccgt caatcgccag ttttccatct gctcctgtgg
    13741 ttagacctga ctgcagaacg gttccattgg catcttgtaa ct
//
LOCUS       DAZKBH010000071        13744 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000071 DAZKBH010000000
VERSION     DAZKBH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.071"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..1771
                     /locus_tag="ACG8OO_001951"
     CDS             197..1771
                     /locus_tag="ACG8OO_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9045817.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(1839..3638)
                     /locus_tag="ACG8OO_001952"
     CDS             complement(1839..3638)
                     /locus_tag="ACG8OO_001952"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFP9045818.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            3761..4267
                     /locus_tag="ACG8OO_001953"
     CDS             3761..4267
                     /locus_tag="ACG8OO_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9045819.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            4346..5068
                     /locus_tag="ACG8OO_001954"
     CDS             4346..5068
                     /locus_tag="ACG8OO_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9045820.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(5146..6546)
                     /locus_tag="ACG8OO_001955"
     CDS             complement(5146..6546)
                     /locus_tag="ACG8OO_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFP9045821.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(6716..7690)
                     /locus_tag="ACG8OO_001956"
     CDS             complement(6716..7690)
                     /locus_tag="ACG8OO_001956"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9045822.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            8043..8603
                     /gene="pth"
                     /locus_tag="ACG8OO_001957"
     CDS             8043..8603
                     /gene="pth"
                     /locus_tag="ACG8OO_001957"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9045823.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            8620..12141
                     /gene="mfd"
                     /locus_tag="ACG8OO_001958"
     CDS             8620..12141
                     /gene="mfd"
                     /locus_tag="ACG8OO_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9045824.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            12156..>13744
                     /locus_tag="ACG8OO_001959"
     CDS             12156..>13744
                     /locus_tag="ACG8OO_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9045825.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
BASE COUNT         4254 a         2600 c         2920 g         3970 t
ORIGIN      
        1 ctagacttaa cgcggctttt tcatgatcat tttctgaaac gaacagtttt gtcattttta
       61 aaagttgttt tgaaacattt ttgtaacata actattatat cgttgacaca ctgctatggt
      121 acaattttta tatcgttaag aaagacttaa atatatttag atatttatta aattaagcca
      181 gtcggaggaa tgaagagtga aaaagagttt aatatcagca gtaatggtat gttcaatgac
      241 cttgactgcc gtagcatcac cgatcgctgc cgcggcggac gattttgatt ctcagataca
      301 acagcaagat caaaagattg cagacttgaa aaatcaacaa gcagatgcac aatcacaaat
      361 cgatgcctta gaaagccaag tttctgaaat caacacacaa gcacaagacc tattagcaaa
      421 acaagataca ttgcgtcaag aatctgcaca attagtaaaa gacattgcag atttacaaga
      481 acgtattgaa aaacgtgaag atacaatcca aaaacaagca cgcgaagcac aagtaagcaa
      541 cacaagctca aactacatcg atgctgtatt gaatgctgat tcattagcag atgctattgg
      601 acgcgttcaa gcaatgacaa cgatggtaaa agcaaacaac gacttgatgg aacaacaaaa
      661 acaagataaa aaagctgtag aagacaaaaa agctgaaaac gatgctaaac taaaagaatt
      721 agctgaaaac caagctgctt tggaatctca aaaaggcgat cttttatcta aacaagctga
      781 tttgaacgta ctgaaaactt cattagctgc tgaacaagca actgctgaag acaagaaagc
      841 tgacttgaac cgtcaaaaag cagaagctga agcagagcaa gctcgtattc gtgaacaaca
      901 acgtctagca gaacaagctc gtcaacaagc tgcacaagaa aaagctgaaa aagaagcacg
      961 cgaacaagca gaggctgaag cacaagctac tcaagcttca tctactgcac aaagctcagc
     1021 aacagaagaa tcatcagcta cacaatcttc aatgactgaa gaatcatcat cagctacaca
     1081 atcttcagca acagaagaat caacaacacc tgaatcttca acagaagaat caacagcgcc
     1141 tgaatcttca gcaacagaag aatcaacaac agcgcctgaa tcttcagcaa cagaagaatc
     1201 aacaacagtg cctgaatctt cagcaacaga agaatcaaca acagtgcctg aatcttcaac
     1261 aacagaagaa tcaacaacac ctgcgcctac aacaccatca acagatcaaa gtgttgatac
     1321 aggaaacggc acaggaagta gtactccggc tccaacgcca acaccaacac ctgaacaacc
     1381 aaaacctgta acacctgctc cagctccttc tggaagtgtg aatggtgcag caatcgtagc
     1441 agaagcttac aaatatattg gtactccata tgtttggggc ggtaaagatc caagtggctt
     1501 tgactgctca gggttcacac gctatgttta catgcaagta actggtcgtg acatcggtgg
     1561 ttggacagtt cctcaagaat cagctggaac aaaaatttca gtttctcaag caaaagctgg
     1621 agacttgtta ttctgggggt cacaaggcgg aacttaccac gtagcgattg ctttaggcgg
     1681 aggacaatat atccatgctc ctcaaccagg cgaaagcgta aaagttggtt cagttcaatg
     1741 gtttgcccct gactttgctg tcagcatgta agaatgatta atcaaaaaga gttagcccat
     1801 tctggactaa ctcttttttt attgtgataa aatagaaatc agataaattc ttctgtcaat
     1861 tccatgaacg tttggatatc ttcagtcacc atgtcgatac ctgcttgcca aaaatctggt
     1921 tgggtaagat cgacattcaa atgtttcttc gcaagatctt ccgtcgtcat tgaggcagta
     1981 tcgcgtagta aatggatata ttcttcttca aaatttgctc ccttttggtt ggcgtaagca
     2041 tagatgccca aactaaagag gtagccaaat gtgtatggga agttataaaa cggtacgtca
     2101 tcaataaaga aatgcaattt tgcagcccag aaatatggat ggtaagtagc taaagctcct
     2161 tgatagcctt ctttttgcgc ctcaaccatc atctctgtga tcttatcttc tgagactaat
     2221 ccttcttgcc gtgcttcata gaatcgattt tcaaaaatga atcgggaatg gatattcatg
     2281 aacatagcta aagcattttg cagttttgta tctagcaaat tgattttttc ttcttttgtt
     2341 tttgcgactt ttaatgtagc atcggctacg atcagttctg cgaatgtact tgctgtttct
     2401 gcgacattca tggcatattc gcgatttaaa gaaggtaaat cccacatcgt gctgctatgg
     2461 aaagcatgtc ctagttcatg agcaagtgtc gcaacctcat taacagaatt actgtaggtc
     2521 ataaagatcc ttgattcttt tgtttctggt aactcagtac aataaccacc aggacgtttc
     2581 ccaggacgat cttctgcttc gatccagctc ttttcaaaag cagattgggc aaatttagcc
     2641 atttttggac tgaatttgtt aaagttttcg ataataaatg ctgctgcttc gtcaaaacta
     2701 aaggtttttt ctttcaaatc gcctaaaata ataggtgcat cttgatcttg ccattccatt
     2761 ttttctttac cgaaaagatt agcttttcta gtcaagtaat taacaagggg ttgtttgttt
     2821 ttctggattg tatcccacat gacagacaat gtttctttct tcaaccgatt gtactctaat
     2881 ggtttttgta gaaaatctgt gacaccatga agttcatagt cagaaagtct aaaaccatct
     2941 aaatgattca aagtatccgt gaagaggggt gccttttctt tccaggcttc ttcccatgct
     3001 gcgaaaagtg tttctctgac ttttggatct ggatcgccca tcatcttatt gaaggcttgt
     3061 ccagcagata attcgactac ttgtccatct tgttcgaagg gaatcgaaat gctggctaca
     3121 atcgtatcat aatgactgct ccaagcattt agaccatcta aagaaagcgt atttataatg
     3181 ttttcttcag cttctgataa taattgtgag ccatcacgac gaatctcgtt taaacgaaaa
     3241 gcaattgttt caaacgaaga ttgagaaagc atctgattcc aatcattgtc agatatttct
     3301 gcaaatttct ttgacaatac agtttcggct gattgcagac gaggcagcat tgcgtaaagc
     3361 ttgcctgata agacctttgc atctgaatca ttgacattgg cagaaagcaa tgcagtaata
     3421 tagctgctgc attgggtaaa accattcgta atcgtttctt gcaattgaag aatagcgttt
     3481 aactgctctg cattgtcaga actaggtgac cattttgtta cgcggtgata atactcgttc
     3541 atttggtctt ccaattgctt catccgttgg ttcaaagcat cagaatgact gcctccggaa
     3601 aaaatagaat ctagattcca atttaatgaa taactcatga aaaaactccc ttcatattgt
     3661 aatacaaaat taagtatagc acttttttat ggaagaagtc tctatgaaaa tgattgttgt
     3721 ttcttcaaaa aaagtctaag ataaaataca gaggtgactt atgaattctt caaaaaaaca
     3781 aatgaaaaac ggaaatattt atttagtaat cgcactgata gtcgttataa tcttatttta
     3841 cagttcttct caaacttacg gacagcaatc acaagtttcg cgattagaaa gctggctgcc
     3901 taatcagcca tttaaagaca cattagcggg tatacgtttt aattatggtg gaagtatggt
     3961 gagtatcaac catctgggct acgccaaatt tttagagttc tttttaagga agggcgcaca
     4021 tttcggtacc tattttattt tgggaggcag cttgttttta ggtgttttcc ccaagctaaa
     4081 aatttggtgg ctgacagcga tccttgcttg gctttcagca acagggtacg caggtttgga
     4141 cgaattccat cagatgatga ccggtggacg aacacctatg ttccaagatg tcatgttgga
     4201 ttcgatcggt gctctgactg ctgtgatatt atgtgtgctt tttgcggcat tcaagaagcg
     4261 tttatagatt ggctgtggct ttgttttttt gtgaaatagt actagaatag aagtgtattc
     4321 tcaaaagaac aagggaggaa aaatcatgtc aggacatagt aaatggcata atatccaagg
     4381 aagaaaaaat gcccaagatg caaaacgagg gaaagtcttt cagaaacttt ctcgtgaaat
     4441 ttatatggca gctaaagcag gcggtcctga tccatctatg aatccagctt tgcggcttgt
     4501 tgtggacaaa gcaaaagctg caaatatgcc taacgacaat gtggaacggg cgattaaaaa
     4561 ggctacgagt gccgtagatg aaacgaatta cgatgaaatc acgtatgaag gatatggtcc
     4621 gggtggagta ggaatcttag tccatgcatt gacagataac cgtaatcgga cagcgacaaa
     4681 tgtccgagta gcttttactc gaaacggtgg atcactggga gaaacgggtt cagtcagtta
     4741 cttgtttgac cgcaaaggat atatcgctat tgaacgcgat gggttgactg ttgatgaaga
     4801 gacgatgttt gaggatgtat tagaggcggg ggcagaagat ctgcaaactt ctcctgaagt
     4861 attcgagatt tatactgcac cagaagattt tgctgctgta cgagatgagt tggaaaaaga
     4921 gttcactttg gcgcaagcag aattgacgat gattccacaa acgacgattg atttaaatga
     4981 ggaacaaaaa gagcaattgg aacgtctagt agataagcta gaagacgatg atgatgtttc
     5041 agaagtcttt actgctgctg aaatgtaagt agcacattgt tagataataa aaagaaagca
     5101 cctgtaccaa tcgtacgggt gctttctttt tatatattat ttttgttaga agaccaatac
     5161 aggcgttcct ttttcgacca tcccgaataa ttccttcatt acacttggtg gggtgttgat
     5221 gcagccgtgt gaaccgcggg ttttccataa gtctccaccg tattcaggtt gccagtctga
     5281 atcgtggata ccaacacctg tccagtcaat cggcatccag tagtttaccg gactctcgta
     5341 aggtgttcca tcgtcatttg ttccttttaa agttgcatct tcttctttat tccaaacata
     5401 gaagactcca gcaggtgtag gtgtagttgg ctttccggaa acgatatctg tttcaagggc
     5461 cacttttccg tctttgtaat accacatatg ctggttctcc agatcaactt cgatatacgt
     5521 atcttcgatc aatggatgat cagcggttgt gccaccttgc acaatgggtg aacgagtaaa
     5581 atcttggcct gccagaatcg cttttttcaa tgcttctgtc tcactgtctg tttggatcgt
     5641 ccaactgtaa gtgccaactg ggaccgtaac ttctcctctt tttgtacttt taaattttgt
     5701 gtcattcgta cttgtgttat actttgtgcc taaatctgtt acatattgac gtacttgttc
     5761 tgtatctaaa tcgacttttc catcattgta ggtcagccaa ctcataatat cacttgatgg
     5821 gatctggaaa gtctcaccgt ttatactgta agtagctttt acatttgcga tagcattcat
     5881 ggatgcgagt tgttctttca gtgagctgtc ttcagatgtt acttttggtt tttctttgaa
     5941 ttcagtcaat tcaattgtat ctttaccaga gtttaccgca ctttttaatt gtttgaccgc
     6001 agcatcgacg tcgatcgtat ttccattgac ttcaggcttg ataacgaaag agtcacctgc
     6061 tttttcgatc gttgcatcag tagtaggtgt ccggtctttg ttcaaatcag tcagcttagt
     6121 tgtcaatgtc tgaacggtag tgtctagttt ttggtggttt tgactgacag gatctatttc
     6181 ttgtttttct gccgctgaga cataagtcat tccccaagtc catccgtttt gttcatttag
     6241 gatcggagaa agctccgaag tgaaatcatc tttgtagccg agctcggatt ttggtacttc
     6301 ctgccagact tttccatctt ctttaatagc cagtttttta tcagaataag cttcttttaa
     6361 tttttcattt gctttttcga ctgttagatt gctgacattt attccattta tttctgtatt
     6421 cggcaaaaaa tgctttgtgt agtatgtact gcgataggta taaaagctga tcagcactaa
     6481 gagaacacca agaactgaaa gcaaagcaac atgtttcgat ttttttcgat gcgaagatct
     6541 tgtcatatcg acaactcctt aagtaaaagt ttagggtgga acggtctcat gatagcataa
     6601 tttagaggaa aagacgaagg ttatctctac tttatgaaaa aatttagaaa ccataagggt
     6661 gataaaataa aaaaacggaa aacaccgaat agatgttttc cgtcatgttc aaagattatt
     6721 cagcgtctag tttagcaaaa gcttcatcta gaatttcttt taattgctta gcagaagcag
     6781 ccatacggtc ttgctcgctg tcacttaaag gaatttcgat tactttttgg ataccttgac
     6841 ggttgataac agcaggtgca ccaatgtaga tgtcgttcaa tccatattcg ccattcatga
     6901 atactgataa aggaagaaca gcattttcat catctaagat ggcacgagtg atacgtgcta
     6961 atgctactgc gataccatag aaagtagcgc cttttttctc gataattgtg taagcagcgt
     7021 cacgtacgcc aaagaatagg ttaaccatag cttcttcatc gatttctggg ttgtttttta
     7081 cccattcata gatttgcaag ccggcaacat ttgcatgtga ccaaactggg aattctgaat
     7141 ctccgtgttc acctaagatg taagcatgga cgttacgtgc atcaacgtca actaattccg
     7201 caagtgcttg acggaaacga gctgagtcaa gagaggtacc tgaaccgatt acgcgttctt
     7261 tagggaaacc tgagaatttc caagttgagt aagtcaaaat atctactggg ttagcggcaa
     7321 ctaaaaagat accgttaaat cctgattcaa cgatttgtgt gacaacatcg cggttgattt
     7381 ttaggttttt gtttactaag tctaaacgag tttcacctgg tttttgtggt gcgccagctg
     7441 taataacaac taagtcagca tcatgtgcgt ctgcgtaagt agcagaatag atttttttag
     7501 gtgaagtaaa tgccaaagcg tgagacaagt caattgcgtc tccttcagtt ttagcagtat
     7561 tgatatccac aatacctact tcttgagcga tattttgagt aactagagca aaggcatagc
     7621 tagaacctac ggcgccgtct ccaacaagaa taactttttg atgatctttt ttttctgcag
     7681 ctgcagtcat ttagtatcaa tccttccatt gatttgtttt ttcccatgct tatgctaaca
     7741 tttctcaagc cccatgtcac gctatttggc tataaaatat aacagataac aaggttgtat
     7801 tatatgtaaa agcttacatg tgcgttattt cactattttt atatatcaat tatttcatta
     7861 aactttgaag aaatttttaa tttctttatc atttattaac gattctcttt tagccgaaac
     7921 gccttgtttt gtgttatact atgcattgac aagtgtaaaa aagaaaaaag ctcgatggac
     7981 tggaccgcta attagcagtc cagccttttg tgctgctttt tagaattagg tggaaaacaa
     8041 aaatgaaaat gatcgtcggg ttaggaaacc caggtaccaa ataccaatat acaaaacaca
     8101 atatcggctt tatggtcgtg gataaaattg cacgcgagca tcaagctact ttcaaaaaaa
     8161 atccgttcga agcagaagtt gccgaatttt ttcataatgg agaaaagatc cttttagtca
     8221 aaccacaaac attcatgaat gaatctggtc gtgcagtcgg cccactaatg acttattttg
     8281 ggatttatcc agaggaatta gtcgtaatct acgatgattt ggatctggca gtgggaaaaa
     8341 tccggctccg tcaaaaggga agtgccggcg ggcataacgg tatcaaaagt attatttccc
     8401 atttgaatac gaatgtcttt gatcgaatca aggtgggaat cggcaggcca gaagggaaaa
     8461 aaacagtagt ccagcatgtg cttagtcctt tcagcaaaga aaatcagcct ttgatagaag
     8521 aaagcatgtg tcaatctgtt aaagcagtcg aatatctcat agaagggcat tcgttcgtgg
     8581 atgcgatgaa tcgattcaat tgaggaagga ggaaaaggaa tggatattat tcaattagtc
     8641 ggaaaagatc cgcaagtaaa agaatgggtc agcaatttag aagaaaaaat gcctagacaa
     8701 ctaatcacag gattagcggg ctcagcgaaa acacttctat ttgctcgagc gtttaagaaa
     8761 acaaataaaa acatgattat tctgatgcca aatttgtatt atgcgaatca gttagcagaa
     8821 gatctgcaac atgtgattcc gcaagatcag atccatttat ttccagtgga tgaagtgctt
     8881 tctgctgaaa tggccttttc ttctccagaa gcgcgaggcg agagagttgc aacgcttaac
     8941 gcacttcaag aaaaaaaagc aggtatctat ttgttgccag tcgcatcttt gcacaaacga
     9001 ctgccgaata agaaaacttg gctacaggcg caattgaatt ggcatattgg tgatgagata
     9061 gacgtggaaa atcttcctcg ccaattgact ttgatgggct atgtacgaga agagatggtc
     9121 gcaaaaccgg gagaattcag tattcgagga agcattatcg atatttaccc gttaacgaca
     9181 acctatcctg ttcgtgtaga actatttgat gtagagatag attctatgcg gtattttgaa
     9241 gcagaaacac aacgctcaat cgaaaaaacg gaagaaatct gggtttcccc aacgagtgaa
     9301 caagtctacg gaccagagga tctagaacat ggaatagagc aaatgcaaaa aatgctagaa
     9361 aaacggcttg ctgcgaccac agaagaagca gaccgcgagt ttttgcaaga ttacttcggt
     9421 cagctgactt caagctggca agcaggtgtc ccgacagaag aagcaaaatt ctactcagat
     9481 cttctttacc aagaatctgt gacgatcttg gattatttcc cttcaaatag tttactagtc
     9541 gttgatgatt atcagcgaat catggaaaca aatcgggaaa tcgaaaggga agcggcagaa
     9601 tggcagactc aaaaaatcag cgaattgcgg gtgttttctg agcagacatt ttcagcagat
     9661 atacaaaaaa tcgtgcagaa ggaaaagttc accacaacct ttttctcact cttccaaaaa
     9721 gggatgggaa atcttcgttt ccaagcaatc cataattttc aatatcggac gatgcaacag
     9781 ttcttcggac aaatgccatt gctgaaaaca gaaatggacc gttggcgcaa gcaaaaacag
     9841 actgttgtga tctttattcc gacaaaggac agaatacgca aagcagaaca aatgcttcga
     9901 gatgaagaca tattagtagt agaaacagaa aaaaatgcat tgatcagagg acaggtacaa
     9961 ttagttgaag gagctttgca ggctggtttt gaacttcctc aagaaaaaat agtagctatc
    10021 acagaaaaag aaattttcca aaaaacgacg aaaaaacaaa cgagacgtca aacagtaaca
    10081 aacgcagagc gattgaaaag ctataatgag ttaaaagcag gagattacgt tgtccatgcc
    10141 aatcatggta tcgggaaata cattgggatg gaaacgctag aagtagatgg cgttcaccaa
    10201 gactatatga ctattttgta tcaaaacgac gacaaactgt ttattcctgt tactcaactg
    10261 aatctgatcc aaaaatatgt cgcctctgaa gccaaagcgc ctcgcatcaa taagctaggc
    10321 ggaagtgaat ggacgaaaac caagcgaaaa gtctcttcaa aaatcgagga tatcgctgac
    10381 gatctaatca aattatatgc cgcaagagag tcagaaaaag gatatgcttt tggacctgat
    10441 gacgcctacc aaaaagaatt cgaaaatgcc ttcccctact ctgaaactga tgaccagttg
    10501 agaagtgcag cggagatcaa acgggatatg gaaaaagaaa aaccaatgga tcgactactc
    10561 gtaggtgatg ttggttatgg gaaaacagaa gtagcgcttc gagcggcctt taaagcgata
    10621 aaggaaagca aacaagtagc ctttttagtt ccaacaacga tcttggcaca gcagcactat
    10681 gaaacgatgc tggaaagatt tgaaggtttc cccgtaaata tcgggctatt aagtcgtttc
    10741 agaacaagaa agcaacaaaa agaaaccatc gaacaactgc gtacaggtca agttgatatt
    10801 gttgtaggaa cccaccgaat tttgtcaaaa gatattgaat ttagcgattt gggtctactg
    10861 atcattgacg aagaacaacg ttttggtgta aaacataagg aacgattgaa acagttacgt
    10921 gcacaagtgg atgttttgac tctgactgca acaccgattc cgagaacttt gcatatgtcg
    10981 atgttaggcg tgcgggatct ttcagtaatc gaaacaccgc ctgcgaatcg ttatccgatc
    11041 caaacgtatg ttatggaaaa aaatcctggg gctattcgcg aagcaatcca tcgagaaatg
    11101 ggacgaggtg gacaagtatt ctatttatat aatcgggtag aaacgatcga acaaaaagta
    11161 gaagaaatcc aagaactcgt tccggaagca cggatcggtt atgctcatgg gcaaatgaca
    11221 gaggcgcaat tagaaaatac gctttttgaa tttattgaag gtcaatatga tgttcttgtc
    11281 actacaacca tcattgaaac aggagtagat atcccgaacg caaatacgtt gttcgttgag
    11341 aacgctgatt atatgggatt atccacgtta taccaattgc gagggcgggt aggaagaagc
    11401 agccgtgtcg catatgctta ctttatgtat gagcagcaga agatccttaa cgaagtcagt
    11461 gaaaaacgtc ttcaggcaat caaagatttt acagaactgg gatctggatt caagattgcg
    11521 atgcgggatc tttccattcg cggtgcaggt aatctcttag gtgctcagca gcatggattt
    11581 attgatgcag tcggtttcga tatgtattct caaatgctaa gcgaagctgt ttctcgtaaa
    11641 caaggaaaga atagtcaagt ggagaagacc actgttgaga tcgacctcgg tgtcgatgcg
    11701 tatctacctg aaacatacgt ggcagatcaa cggcagaaaa tcgagatcta taaacgaatt
    11761 cgtgaacttg attcgcaaga aatgctagat gaactagaag atgatctgct cgaccgtttt
    11821 ggagaatatc cagaagaagt agcacatctc ttgacaattg ggcagatcaa aatggatggc
    11881 gatcgagcgc tcctagaaac gataaggaaa caggaggcgc atatagtctt tactctaagt
    11941 aaagttggaa caaaacgcta tagtgtggaa cagttattcg aagcactgac agcgacaaaa
    12001 ctaaaagcca gtctaggcgt tgaaaaagaa cagatggtga tacgcttgac gatccccaac
    12061 caaatggaag aagcagtatg gcttcaggaa atccaaaagt ttgtcaaagc gttgcgtgag
    12121 caaaaatatc tgtcagcgta ggaaggagca atgatttgga tcataaagaa atgcggcgcg
    12181 tcatgcaagg agcatttgta ttgacgattg cttctttcat agcaaaagta ttaagtgccc
    12241 tttaccgaat tcccctccaa aacttagtcg gcgacgaagg tttttatgtt tatcagcaag
    12301 tttatccaat ttatgggata gcaatgactt tagctttatc cggtcttccg cagttcatat
    12361 caaagtacgt tgcagaaaga aagaatcctt tcgagcgaaa gcaagctttg caaaatcttt
    12421 atcccttagt attttggaca ggtgtctttt tgtggttgtt tgtctttgca tttagcagct
    12481 ggattgcttg gatgatggga gacatacaat tgaaaccgct gatccaagta gtttccttta
    12541 cctttttgct gatgcctggt ctatcttttt atcgagggaa tttccaagga agattcttga
    12601 tggaaccaac tgctgtttct caagtagttg agcagttcgt tcgtgttttt gtgattgtct
    12661 gtagtgcgtt tgcttttaga aagcttggat ggacggtgta ccaaacaggg acggcagcaa
    12721 tgagtggagc tgtttttggc ggtttatgcg catatgggat ccttcactac tatgaacaaa
    12781 agattcatgg aggagcgtta agcttccgac ggtttccatt gttcaaacag ccgtcaaagc
    12841 ccttgatccg gcgttttctg gtagaaggcg gactggtttc gatttatagt ggattgctta
    12901 tctttttcca gctgatcgac tctttctttg taaaaaatgc tttggaagtt tacggactgt
    12961 cagaatacgg tgcgaagata gcaaaagggg tctatgatcg aggtcagcct ttagtacaat
    13021 taggtttagt gattgccact gcattaagtg cgacattcct gcctgcgttg actcgtcatt
    13081 tgaccaaccg gatctatcgt cagtttttac agacagccaa aatctatctt agattgacga
    13141 ccgctttagc attagctgcg tctttgggat tagccttgct gctcccttat atcaattatg
    13201 cgttgtttaa agattatgcg ggaaatgcag cgcttgtatt atttgttttt tccattgctt
    13261 tcactgcagt catccaagct tatcaaagta ttgctcaaag caagaactcg tttcgccctt
    13321 ctttaaaagg cgcaggatgg ggactattgg taaaaggatt gactacttct tttctgaccg
    13381 gtttattagg aacagcaggt gcaagcctgt ctaccctttt aggattaggt gtgacgcttt
    13441 ggtattttgt tcgaattgaa acaaaagagc taaatgcttt ttggaaagaa cggcattttg
    13501 gacggaagtt gatgttcagc cttctttgta tgacagtagt actgctgctg tattatggtg
    13561 tattgaatat agtatttggt cctgtaactc atcgttccac tgcttttttt gtcagcgtga
    13621 taggagttgg gatcggggct tctgtgttca tttatgcagt gattcgtctt cgtctgttta
    13681 cagtacgaga atggctaatg ttgccttttg gtaagaaaat cctgcgatta aataaaaaaa
    13741 ttaa
//
LOCUS       DAZKBH010000072        13398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000072 DAZKBH010000000
VERSION     DAZKBH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.072"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..712)
                     /locus_tag="ACG8OO_001960"
                     /pseudo
     CDS             complement(14..712)
                     /locus_tag="ACG8OO_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            791..967
                     /locus_tag="ACG8OO_001961"
                     /pseudo
     CDS             791..967
                     /locus_tag="ACG8OO_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="ACG8OO_001962"
     CDS             complement(1143..1712)
                     /locus_tag="ACG8OO_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9045826.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ACG8OO_001963"
     CDS             complement(1786..2109)
                     /locus_tag="ACG8OO_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045827.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ACG8OO_001964"
     CDS             complement(2263..3075)
                     /locus_tag="ACG8OO_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045828.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3402..4277
                     /locus_tag="ACG8OO_001965"
     CDS             3402..4277
                     /locus_tag="ACG8OO_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFP9045829.1"
                     /translation="MSKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4291..4614
                     /locus_tag="ACG8OO_001966"
     CDS             4291..4614
                     /locus_tag="ACG8OO_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045830.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4611..5363
                     /locus_tag="ACG8OO_001967"
     CDS             4611..5363
                     /locus_tag="ACG8OO_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFP9045831.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="ACG8OO_001968"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="ACG8OO_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ACG8OO_001969"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ACG8OO_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFP9045832.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="ACG8OO_001970"
     CDS             complement(9827..12013)
                     /locus_tag="ACG8OO_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9045833.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ACG8OO_001971"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ACG8OO_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFP9045834.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="ACG8OO_001972"
     CDS             complement(12235..12675)
                     /locus_tag="ACG8OO_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFP9045835.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="ACG8OO_001973"
     CDS             complement(12749..13288)
                     /locus_tag="ACG8OO_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9045836.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DAZKBH010000073        13212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000073 DAZKBH010000000
VERSION     DAZKBH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.073"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG8OO_001974"
     CDS             147..1016
                     /locus_tag="ACG8OO_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9045837.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG8OO_001975"
     CDS             1225..2250
                     /locus_tag="ACG8OO_001975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9045838.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG8OO_001976"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG8OO_001976"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFP9045839.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACG8OO_001977"
     CDS             3333..5036
                     /locus_tag="ACG8OO_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9045840.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG8OO_001978"
     CDS             5038..7071
                     /locus_tag="ACG8OO_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFP9045841.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG8OO_001979"
     CDS             7082..7522
                     /locus_tag="ACG8OO_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9045842.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG8OO_001980"
     CDS             complement(7722..8849)
                     /locus_tag="ACG8OO_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9045843.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG8OO_001981"
     CDS             complement(8852..9529)
                     /locus_tag="ACG8OO_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9045844.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACG8OO_001982"
     CDS             9793..11559
                     /locus_tag="ACG8OO_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFP9045845.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACG8OO_001983"
     CDS             11574..12011
                     /locus_tag="ACG8OO_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9045846.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACG8OO_001984"
     CDS             12024..13175
                     /locus_tag="ACG8OO_001984"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9045847.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAZKBH010000074        13183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000074 DAZKBH010000000
VERSION     DAZKBH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.074"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1811
                     /gene="yfcC"
                     /locus_tag="ACG8OO_001985"
     CDS             72..1811
                     /gene="yfcC"
                     /locus_tag="ACG8OO_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9045848.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1968..3152
                     /gene="iadA"
                     /locus_tag="ACG8OO_001986"
     CDS             1968..3152
                     /gene="iadA"
                     /locus_tag="ACG8OO_001986"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9045849.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3263..3466
                     /locus_tag="ACG8OO_001987"
     CDS             3263..3466
                     /locus_tag="ACG8OO_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9045850.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3567..4229)
                     /locus_tag="ACG8OO_001988"
     CDS             complement(3567..4229)
                     /locus_tag="ACG8OO_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9045851.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4242..5744)
                     /locus_tag="ACG8OO_001989"
     CDS             complement(4242..5744)
                     /locus_tag="ACG8OO_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9045852.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6292..7971
                     /locus_tag="ACG8OO_001990"
     CDS             6292..7971
                     /locus_tag="ACG8OO_001990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9045853.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8098..8577
                     /locus_tag="ACG8OO_001991"
     CDS             8098..8577
                     /locus_tag="ACG8OO_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFP9045854.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8796..9476
                     /locus_tag="ACG8OO_001992"
     CDS             8796..9476
                     /locus_tag="ACG8OO_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9045855.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9484..10815
                     /locus_tag="ACG8OO_001993"
     CDS             9484..10815
                     /locus_tag="ACG8OO_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFP9045856.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10830..11792
                     /locus_tag="ACG8OO_001994"
     CDS             10830..11792
                     /locus_tag="ACG8OO_001994"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFP9045857.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11903..12241
                     /locus_tag="ACG8OO_001995"
     CDS             11903..12241
                     /locus_tag="ACG8OO_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9045858.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12320..13027
                     /locus_tag="ACG8OO_001996"
     CDS             12320..13027
                     /locus_tag="ACG8OO_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFP9045859.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4185 a         2332 c         2897 g         3769 t
ORIGIN      
        1 actgtataat tcatttgatt atccattttt tattcatcaa aattactgaa aaaagaaagg
       61 aagaaataaa tttgaagaaa acaaattctt caggaaaacc gaaacgcgac ttcactaaat
      121 tatcgacacc ccatacgtat gtcattattt ttggtgtagt catctttgca tggatactga
      181 cttttgtcgt tccagcagga aagttcagca cgcaagatat tgaatacaaa gatgcaaatg
      241 gcgaaacaag tactcgtact gttttacgtc aagattcctt ccgttatgct tacgagctgg
      301 ataagtcgta tgtgtttgat caattagaag aactgcaaga tcatccagca gaacgtgaaa
      361 aactggatgt tccggaaaaa ggattggaaa aagtcattgc tgacggagaa aaaaatctta
      421 cgcaggaaaa actagatgaa atctccttga cagatgatgt gctttacgat cagtacggcg
      481 aaaatatcta tgatacttct aaaaaattgc ataaaacagc caaaatatgg ggaacagatg
      541 attttggcgg ctttggtttc ttaaattttg tttttgaagg actagtatct ggagataagt
      601 atggatctgc tgtagggatc gctgcattga tccttgttgt tggaggtgct tttggtatta
      661 ttatgcgtac cggagcgatc gacgcaggta tctatgcgtt tatcagtaaa actaagggac
      721 tggagcgttt agctttaccg ctattattct ttgctttttc attcggtggc gctacattcg
      781 ggatggcaga agaggtaatt ccattctcaa tggtgatggt tccatttgtc attgcgttag
      841 gatatgactc gatcgtagca gtaaccgtga cttatgtcgc atctcaagtc ggaaatgcga
      901 cctcttggat gagcccgttt agcgtagcgg tggctcaagg gattgctgga atccccgtgt
      961 tgtctggtgc gacattccga ttgattatgt gggtagttgt tactgcatta gccgctggtt
     1021 atatgatgat atatgcagaa aaaatccgta aaaaaccaga aagttcgttg acttacaaat
     1081 cagatgatta tttccgttct catatcaaaa agacatctga tgaaaataaa aaattcatgc
     1141 ttgggcataa attgatctta ttagagatgt tagttgtcct tgtctggatc gtttggggcg
     1201 tgactcaaaa aggctattac attccagaaa tcgcttctca attctttgtg atgggattag
     1261 ttgctggaat cattgctgtt cttttcaaat tagatggaat gaaagtcaat gacatcgctt
     1321 cttctttcca atctggtgca gctgatttag ctggtacagc aatcgtagta ggtatggcca
     1381 aagggattct tcttgtcttg ggaggatctg atgcatctgt tccttcagct ttgaatacga
     1441 ttttgcatgg gatcgggacg gcgttgaccg gtgttccagc tgtcattggt gcttgggcga
     1501 tgtacatctt ccaaagtttg ttcaacttgg ttgttacatc aaattcagga caagcagctt
     1561 tgactatgcc aatcatggct ccactggcag atttagtagg tgtatccaga caagtagcag
     1621 ttcttgctta tcagcttggt tcaggtttta tggacgcttt tacacccgta tcagccagct
     1681 tgattggtgt gctaggtgtt gcacggatcg aatgggcaaa atgggcaaga ttccaaatca
     1741 agatgcaagg attcctgttt gttttaggaa ctattttcat catgattgcc attgcaatag
     1801 gtctgcagta gtactggaga cctgtaaata aaaaagtaat ccaaacagta gataaggact
     1861 ccaagaaccg tcattttacg gtaaaatcaa aggagtaaag atcctatcgt ttggattatt
     1921 ttttaagatc ctatcatcag ttatacttaa agaaagagga aaaatgtatg aaaatcattc
     1981 gacaaattga agtgtttgca ccagattact taggaaaaat ggatgtactg attgccggtg
     2041 ggaagatttt ggctgtagaa gaacagctag aaggcggcta cgaaggagtg gaagtagaag
     2101 aactttcagg tgagggaaaa gtactaaccc cgggtttcat tgactgtcat ttccatattt
     2161 taggtggagg aggagaaggc ggttatcaaa atcgaacgcc agaagtcaca ttgagccaat
     2221 tgactacagc tggtgtaacg acagttgtcg gttgtttggg aacagacgga gaaggtagag
     2281 atatgactgc gttgatcagc aaagcgaaag gtctggaagc agaaggaatc tccacttatg
     2341 tatacgaagg ttcgtaccga ttgccggtaa aaacagtaac agattctatc atcaaagact
     2401 ttttgacaat tgataaaatt atcggaatcg gagaaatcgc agtatccgat caccgttctt
     2461 ctcagccaag ttttgaagag ttcgctcgtg cagtagcaga tgctcgtgta ggaggcatgt
     2521 tatccggaaa agctgggatc atcaatgtcc acttaggcgg aggaaaaaga aagctagagc
     2581 tattgacacg aatcgtagaa gaaacagaaa ttccgatcac acaatttttg cctactcatg
     2641 caaatcgcac accagaatta tttgaagctt gcgtggcatt tgccaaacgt ggtggcacga
     2701 ttgacttcac tgctagcgaa gatcctgatt tttgggagaa gacagatggt gaagttcgct
     2761 tcagcaaagc actgaaacga ttgatcgagg aagatgtttc tttagataat ttcacgatga
     2821 cctcagatgg acaaggaagc ttgccttatt ttgatgaaaa caatcatttc ctagggttag
     2881 gagtaggaag tgccaaggca ttgctagtag ggatcaaaga agctgtgcaa aaagaaagta
     2941 tccctttgga aatcgcctta cgagctataa ccagtaatcc agcacgtata ttgaaattgg
     3001 ataaaaaagg aaaaatcgaa attggtgctg atgcagatct gtgtatcttg gataaagaaa
     3061 cgcttgatat tgatacagtt atcgctaaag gagaaataat ggtccaagaa aaagaagtca
     3121 aagtatgggg gactttcgaa aaaagttttt aatcaacagc tctaactatc ttttttagaa
     3181 aacgagcgtt ctgtttgtct gtatctgttg ttgatgttac cctgaaggta gatcaataaa
     3241 caaactaaga ggaggaagca caatggcaga tttaaaagga cgttctaaag atatgaaaga
     3301 taaagtagtc ggtgaaggaa aagaagcata cggcaaagca acagacgaca agagcaaaga
     3361 aacagaaggg aaagcacaat cactaggtgc tgacatcaaa gaaaaagctc gaaatttagg
     3421 cgacgaagtc aaagacaagt tttctcgaaa agacgaaaaa aaataaaaac cacttattac
     3481 taattgaatc aagcaaataa aaaatccgaa ccatatctgc gagacagata tggttcggat
     3541 tttttatttg atcattatcc tgttttttaa acttcgattt tttgaaattt tttatttttt
     3601 agttcaaaga cttcatcaca gctttttgct acttcaatct catgagtcac gatgataata
     3661 cagcgatttt gttcatgggc gatttcttgg aaaagtttga cgatatcttg tgaagttgtt
     3721 tcatctagat ttccagtagg ctcatccgct acgattagtt gatgttcaca gcagatagca
     3781 cgaacgatag cgactcgctg ctgctgacct cccgaaagtt tggtcacctg tttttttgct
     3841 aatatttcat caattcctac acgagccagt cgatctaaag caacttgtcg ataattctct
     3901 tgcttggtct tagtgatttc cattgctgct atgacgttgt ctagagccga taaataagga
     3961 agcaagttgt aagcttggaa aataatcgag acatcatttt tacgataact tcttaacccg
     4021 attttgttca gtgaacgatc ttgatagtat acctttcctt cttttggtgc atctagtcca
     4081 gaaataagcg aaagaaaagt cgtttttcca gaaccacttg ctcctaaaat agcgtacatt
     4141 tttccagctt cgaaattcag ttctacatct ttataaagcg catcttgcgg atcttgatac
     4201 caaaatccca attgttctgt ccgtaacatc aaattcctcc tttagtttaa taggattttt
     4261 cttgggttta atcttaatat ccctatagaa gctaaaaaga cagaaaacag actgattcca
     4321 aatgctactc cggctaatag tcccaattgt gcaggtttga cattgatttc caattcgctg
     4381 acttgatcag aaacagcaaa tggattattc gtggttcctt gttgaccatc gcgttgaccg
     4441 ccttgttctt gtctgttacc tggcatttgt ccaccgccag gagcttgctg gttttgtgtc
     4501 gtggtttctg tctgttggtc aagtagctgc tgtcccacgg cattcgcgac gacgtttccg
     4561 ctaagcgaag caactcctaa tgcaagaatc atacaaatag ccacttcggt aaacaattgg
     4621 agaatgattt tacttctgga ttctcctaaa gatagcaaga cgcctaactc atgccgacgc
     4681 tcccgaatgg taatcataat gatcagagta agtatgataa taccggctac agccactagt
     4741 aaaacaacat ttttagaaaa acttgccacg ttgtttaaag gttccaacat tgactgatac
     4801 atggaatcat ttgattgtaa gctaaatgta tctgtgtcga tcagcttctc agcttctttg
     4861 agaaactcat ccattttatc tggatcagat agagtgtaaa tagcagaatc tatcgtgtta
     4921 tcgtcagatg tcccatttag ttcatttgca aaagtgtaag aagtgtaaag tgtattcgac
     4981 ggattcatga agttgaattt catacccata ctgccgcttg tttcgctgga ttcataaatt
     5041 cctttgatcg tcatttcata agtggtatct tcatcttcag tactcgtgat tgtgaaagta
     5101 tctccgactg ccaggtcatt tgtttctgct agagtagaat caatgactac attgtttgtg
     5161 cctttgtcgc tgtcatctat tccttcacca tccgtgattt ttgctgttcc atcagaaaaa
     5221 ttactatttt gtgcagttgc tgacacacca ctgatttgaa agtctgcttg agtcatttgc
     5281 ggattcccgc ccattcctcc agcaggttca ccgcctgcat cagtagtttc tgaactgtct
     5341 tcacttgaat cagaactagt gatggcagtt attccatctt ttgccatagc tgaggcggaa
     5401 ctttcaaagg aataactctt tacattatct aattcagcta ttttttccgc gtccgttaag
     5461 gaaactggtg taagagaaaa actgcctgga tctggacgtt cttcagtggt agaaccgctt
     5521 gaagaagttt gttcctgttt tttaaatgaa gcttctcgat tggcacttaa agtcactgtt
     5581 gctccaactt ctttttgtgc ttgattcgct gcttcttctg cagcgcttcg aatcgtgagg
     5641 ccagctagca caaaaataag aatggctgtg aacacggcaa acaataacaa tgaacgtccc
     5701 cattttactt ttgtactttt ccatgcacgt ttcataaaat tcatattttg catccctcct
     5761 agtagatgca tttgataata gacaacctat cttaaagcta gcttaaatct tatgctgttt
     5821 gcacaccatc aacatgaggt tctgtatata taaaaagtca ctatagttat tgcaaaaaat
     5881 gtggtaaaat gaaaaaagta tgtttcaaac cgacgaagaa gaaatgaaag aggaatagca
     5941 aaagaaatcc tagatgctac agagggattt ttacgtgcat agttgttcta acaattgctg
     6001 atactagcga tttagtttat ttgttgtata ataatgggga acagctgaat acttatataa
     6061 gtattctggc tatagcgttt cgcaaaagat ccattttgtt aatgaagtat atccaaaata
     6121 aaatgcccat ccaactgtca gaacgcggat gtgctggtaa ttttcctgct gattctccat
     6181 aagttgagaa atagtgattt tgcatatgct tcattggaat ggctcatata tttagataaa
     6241 gaagaaaatc gacatcttta aacttggatg tcataagaag aggtgaagtt tgtgagtacc
     6301 attaaaatta tcccattagg cggcgttcgt gaaaatggaa aaaacatgta tgtcgcagaa
     6361 gtgggcgagg atatttttat tttagattgt gggctaaaat atcctgaaaa tgagttgttg
     6421 gggatcgacg tcgtaatccc tgattttacg tatttagaag aaaacattga tcgtgtagct
     6481 ggtgtctttt taacacatgg acatgcagat gcgatcgggg cacttcctta cttgttgtca
     6541 aaaatttctg taccagtatt cgggacagag ttgacgatcg agttagcaaa attgaatgtc
     6601 agtcgtaatg aagcagccaa aggatttaag gatttccatg tggtagatga gcatacagaa
     6661 atagactttg gtgaaacagt agtgagcttt ttccgtacca ctcatacgat accagattca
     6721 gtggggatca atctgaaaac aaaagaaggt agtatcgttt atacgggaga ttttaaattt
     6781 gaccaaacgg ctatcccaat gtatcaaaca gattatgcac gattggcgga aatcggcaaa
     6841 gaaggagtct tagccttact aagtgaatcg gcaaatgcag aaaatcctca gcctgtagct
     6901 tctgaagccc aaatagcaga tgaagtattt gatacgatca aatattggga agggcgtatc
     6961 atcgtcgctt gtgtagcaag taatttgcaa cgagtgcaac aagtattgga tgcagcagaa
     7021 aaagcacaac gaaaagttgt cttgactggt caagatttcg aacgaattat tcgcacagcg
     7081 atgcgtttag aaaaacttca attaccaagt gaagatctgc ttgttacgtt gaaagacatg
     7141 aaaaactatg atccagaaga actgatcatt ttagaaactg gccgaatggg tgaacccatc
     7201 aagtctctgc aaaaaatggc aaacggtaca caccgtaatg tcaagattga agaaggagat
     7261 ttggtttata tcactactac gccaagcatc gcgatggaaa ccatcgtggc gaaaacggaa
     7321 gatatcattt atcgagctgg tggaacagtg aagttgatct ctgagaatat gcgcgtttct
     7381 ggccatgcca atcctaatga cttgcaatta atgatcaatt tgatgaaacc aacatatttt
     7441 gtgccagttc aaggagagta tcgtgaatta gctgctcatg cagatttggc tcatgcagta
     7501 gggatgccat acaaaaatat ctatattact ggacgcggag acgtgctgga atataagaac
     7561 aagcgtatga gtgttgcagg aactacaagt gcagaaaatg taatgattga tggattaggc
     7621 ataggtgata tcggaaacat tgttttacgt gatcgaaaaa tcttgtcaga agacgggatt
     7681 ttcgtagcgg ttgtcactat cagtcggcga gaaaagaaaa tcatttcaaa acctcaaatt
     7741 acttcacggg gattcgttta cgtaaaagct agccgtgatc tgatgaaaga aagcagcaat
     7801 atgattgaaa caatcgttga gaaacattta gaaagcaatg attttgagtg gagcaaatta
     7861 aagcaagaca tccgagatca attaggccgt tacttatttg aacaaacaaa acgccgccca
     7921 gtgatcttgc cagtcattat ggaagctact caacgtcgaa ataaaaaata aacaaaaaga
     7981 gtaagatgaa cctctctata gagtgctcgt cttgctcttt ttttgcgaaa aacaaataat
     8041 ttttgaatac agcatccctc gctttataat aaacgataga ataaagggag tgaatcaatg
     8101 atgagtcaaa caaaagtcga acgttattta gaagaacaaa agatcagcta tcaaccactt
     8161 gattttagtc atgtggtaga agaatctttc acagaagaac tacaaaaaag agggattgat
     8221 ccagctttga tatgtaaaac tctcgtagtg aaaggaaaca aaactggggt gatcatcgca
     8281 ttgattcctt tgaacgatca cttagattac aaaaaaacga gacaagtgac gaaagatcgt
     8341 aaagttggtt ttcctggaat ggattttgta ttaatgcata caggatatcc ccacggagca
     8401 aacaccccta ttggaataaa actcgcacat cctgattatt gttttttagc agacagttct
     8461 ttaaaagaaa aagaagaagt aattgtgtca agcggtgaaa tcgggagaag ccttcggata
     8521 aaagtagccg atttagaaaa gatcgtccat cctatttacg ctgatttaat tagttaaggg
     8581 acgagaagag aagctaagca gagcagagac ctcaaacttg agattatatc tgcttggtac
     8641 ttggtgcatc ctgtattatc tgttacaatt gctttgtaca tactgtacta tcaatccaat
     8701 gaaaatatat ttagcagcta tattttttca ttgatgtgca agtgatgaaa tcgtttttaa
     8761 taaaaaagat acagagaaaa cgcgaggtgt aaaatatgaa aattacgtat catggacatt
     8821 cctgtttaac aattgaaatg gaaaatggcc aacgcgtgat gattgacccg tttattacag
     8881 gaaatccatt gacagactta actgcagaag aagtaaagac agattggttg ctgattactc
     8941 atggtcatag tgaccacata ggagatatga ttcctattgc aaaagaaaat caagccacaa
     9001 ttatcagtat tgttgagatt gctgattatg cgaacagcag aggagtagat tcattcggta
     9061 tgaatatcgg tggaaaacat gcgtttccat tcggcgtggt aaaattcgta catgcacagc
     9121 atagttcaag ctatgaagta gacggtatcg ttcagtatat gggcgagcct agcggaatta
     9181 ttattcaagc agaaggaaaa acgatttatc atgcagggga tactgcttat ttcagtgatc
     9241 taggattgct tgcagaagaa ttcgatattg atgtcgcttt cttgccgatt ggcgataact
     9301 atacgatggg gccagaagat gcagcaaagg ctgcgcaaac aattcgtgcc aagaaagtag
     9361 ttccaatcca ttacaatact ttccctgtca ttgaacaaga tcccgaatct tttgtagagt
     9421 tactggaaga tagtgaagga aaaataatga gcgtctcgga aacgctggaa gtgtaggcag
     9481 aaaatggcga caaaacatga tttgatttta gaatatattg aaagtttacc tgttggcaat
     9541 cgaatttctg taagaagcat cgcaaaagaa ttgaatgtca gtgaaggaac agcgtaccgt
     9601 gcaatcaaag atgcagaaaa tgtcggacta gtttcaacaa ttcaacgagt agggaccatc
     9661 cgtatcgaac gcaaattaaa aaaacatatt gaacgtttga catttggcga ggtcgtcagg
     9721 atcattgaag gggacgtttt aggcggggcg gcaggattag acaaggtctt gaataaattc
     9781 gtcatcggcg cgatgactaa acatgccatg gaacgctata ttacgccagg atcactaatg
     9841 atcgtcggca accgggcgga agtccaaaaa ttagcattgg aagatggcgc cgcagtattg
     9901 atcacaggag gattcaatac gactgctgag attgcaaagc tagctgatga tcttgaaatg
     9961 ccgatcttac gcacctctta tgatacattt acagtcgcaa ccatgatcaa ccgagcgtta
    10021 agcgaccaat tgatcaaaaa agatatcatg cttgtcagtg atatctatat gccagctgaa
    10081 aagactcatt atttgcagca gacagatacg atttctgatt atcagcgttt gtcagaagaa
    10141 acgcagcatt cgcgttttcc agtcgtcaac cgccatcatc gtttgatggg gattgttacg
    10201 gcaaaagatg ttttagggaa atcgccaaac cagatcatcg atcgggtgat gacgaaagaa
    10261 ccaatcagtg taaagaaaac aatgagtatt gcctctgtta gccatcaaat gatctgggat
    10321 gggctagaag tcatgccggt cgtttccgat gacttgacat tggaaggctt gattacccgg
    10381 caagatgtaa tgaaagcgat gcagcttgtc cagcgacaac cgcagattgc agatacgatt
    10441 tcggatcaga tatctggaaa tatccatacg attgatactg accgtgaagg aaatcgtttg
    10501 gaaaacccta agttcaaatt cgtagtagca ccacaaatgg tcaatggcgt tggaaccatt
    10561 tcttttggcg tacttagcga aatcatttct gatgtcgcac aaaaaacaat ggtgatgaac
    10621 caaaaacgaa atatcttgat tgaacaagtg aacctacatt atttgcggct gatccaattg
    10681 gaaagcgaat tagacattcg tccacgtgta ttggaaatcg gcagacgttc cgctaaactg
    10741 gatatcgaag tgtttattga aaacaccatc gtttccaaag caatcgttgt ttgtcaagta
    10801 atggaacgtt cttagaaagc aggagtgaaa tgacagtaca gcaagaaata gtagatacaa
    10861 taaaaaaata cgataaaatc attatccaca ggcatttgcg ccctgatcct gatgcattag
    10921 gatcacaagt aggattagcc gaactattac gggaaagttt cccagaaaaa gaaatacgtc
    10981 aagtaggtga aacgattgaa gggcttcgct ttttagcaga aatgcaagaa gttgaagatg
    11041 tcttctacca aggagcgcta gtcatcgtga ctgatacagc taattcaccc agaatcagtg
    11101 atcaaagata tcagttggca gaaaaactga tcaaaatcga ccatcatccc aatgatgacc
    11161 cttatggaga tcttgtgtgg gtaaatacaa aagcaagtag ctgtagtgag atgattgcag
    11221 aatttgcttt gatgtttcct gaatctttaa agatgaacgt taaggctgca cggttacttt
    11281 atgcaggaat cgtcggagat acagggcgct ttttgtatcc ctccacgacg tcccgtacat
    11341 tagagattgc ttcgatattg agaaattatc catttgatgc aagtgctttg aacagagaga
    11401 ttgaacaaat gccgatgaaa gtcgctaaac tttccggtta tctttatcaa aatatccagg
    11461 tggatgaaaa tggtgcagga agaataatcc ttccgcaaga aattcttgat aaatatggaa
    11521 tcgatgattc agagacagct tcgatcgttt cacttcctgg agtgattgac gaagtgctgg
    11581 cttgggggat ttttgttcag cagccttcag ggtattaccg cgtacgttta cgttctaagg
    11641 ggccgatcat caatgaattg gctaaacgcc atcatggagg aggccatcca ttagcaagtg
    11701 gtgcgaatgc aaaagattta gcagaagtag aggaaatcta taaagagatc caagatatat
    11761 gccaatcgta taagagcaga aaaacagaat gatacttcgt tcgaaaacgt gccttttgta
    11821 gtaaaaatca caaatgtatc ttttcaagat cttctttttc cgttacaata gtgctcacta
    11881 aagattttaa aggagaaaga agatgacatt accaaattgc ccagaatgtg gctcagagta
    11941 cacatatgaa gatcgaggct tgttgatttg cccagaatgc gggaacgaat ggaacaaaga
    12001 agaacaagaa actgtcatag aagacggatt agtggtatta gatgcaaatg gaaaccgtct
    12061 gttagagggc gatagcgtga cagtgattaa agacttgaaa gtaaaaggtg cgtcaggtgc
    12121 cattaaacaa ggaacaaaag tcaagaacat ccgtttagta gaaggcgatc ataatatcga
    12181 ttgcaaggta gaaggatttg gaccgatgaa actgaaatcg gaatttgtga aaaagaatta
    12241 aagaatattc aaggtctttt cacattcttt tcctactact aatgataaaa taaagtaaac
    12301 gaaaaggagt gaaacgaaaa tgtatggtta tggaatgtat ggttttttag atccgacatt
    12361 tcttttggtc attcttggat tagttatttc cggaattgcg tcagcgtacg tcaatagtac
    12421 tttccgaaaa tacgatcaag tgagaagcaa aaacaatgtg actggtacac aagcagcaca
    12481 atatatatta caaagtcaac agataaataa tgtcggtgtt caacaaattg ccggagatct
    12541 cacagataac tacaattcag gtaataagat gttgagtttg tctcaagcaa ctgcacagtc
    12601 tacttcagtt gctgcgatag gtgtagcagc tcatgaatgc ggacatgcgg tacaagatgc
    12661 ttcgggttat gtcccattga agattcgtgc agcacttgtt ccagcagcta actttggttc
    12721 gatgatttcg ttccccttga ttatggtagg tgtattgctt agctggaact ctacgttaat
    12781 caatatcggg attttcgctt tttctttagc attacttttc caattggtga cattaccagt
    12841 cgaatttaac gcgtctcggc gagcaatcca aattttgagt gaaggcggac tactgacgga
    12901 agaagaagta ccgatggcaa gacacgtatt atttgcagca gcactgactt atgttgcagc
    12961 agcgctttcg acattcctgc agctgttgcg cttgatctta ttgttcggcg gcaaccgaag
    13021 agattaaaat aaagaagttg tgaaatcagc gctttgatct gagctaatac cgagagttag
    13081 ctgattgaat accatttatt tggaataagg gctctttgtc aataaggact gatgggttgt
    13141 acaaaatcaa atctgggtca gaatgaacct cattctggct cag
//
LOCUS       DAZKBH010000075        13033 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000075 DAZKBH010000000
VERSION     DAZKBH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.075"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG8OO_001997"
     CDS             414..947
                     /locus_tag="ACG8OO_001997"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9045860.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1437..1961)
                     /locus_tag="ACG8OO_001998"
     CDS             complement(1437..1961)
                     /locus_tag="ACG8OO_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFP9045861.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="ACG8OO_001999"
     CDS             complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="ACG8OO_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9045862.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2550..3530)
                     /gene="pta"
                     /locus_tag="ACG8OO_002000"
     CDS             complement(2550..3530)
                     /gene="pta"
                     /locus_tag="ACG8OO_002000"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9045863.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3683..4363)
                     /locus_tag="ACG8OO_002001"
     CDS             complement(3683..4363)
                     /locus_tag="ACG8OO_002001"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9045864.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4489..5358
                     /locus_tag="ACG8OO_002002"
     CDS             4489..5358
                     /locus_tag="ACG8OO_002002"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9045865.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5384..5899
                     /locus_tag="ACG8OO_002003"
     CDS             5384..5899
                     /locus_tag="ACG8OO_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFP9045866.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5946..6509)
                     /locus_tag="ACG8OO_002004"
     CDS             complement(5946..6509)
                     /locus_tag="ACG8OO_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9045867.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6564..6743)
                     /locus_tag="ACG8OO_002005"
     CDS             complement(6564..6743)
                     /locus_tag="ACG8OO_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045868.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6820..7329)
                     /locus_tag="ACG8OO_002006"
     CDS             complement(6820..7329)
                     /locus_tag="ACG8OO_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045869.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7425..7805)
                     /locus_tag="ACG8OO_002007"
     CDS             complement(7425..7805)
                     /locus_tag="ACG8OO_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9045870.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8135..8884
                     /gene="pnuC"
                     /locus_tag="ACG8OO_002008"
     CDS             8135..8884
                     /gene="pnuC"
                     /locus_tag="ACG8OO_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9045871.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9068..10345)
                     /locus_tag="ACG8OO_002009"
     CDS             complement(9068..10345)
                     /locus_tag="ACG8OO_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045872.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="ACG8OO_002010"
     CDS             complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="ACG8OO_002010"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFP9045873.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11267..12211)
                     /locus_tag="ACG8OO_002011"
     CDS             complement(11267..12211)
                     /locus_tag="ACG8OO_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFP9045874.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12213..12899)
                     /locus_tag="ACG8OO_002012"
     CDS             complement(12213..12899)
                     /locus_tag="ACG8OO_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFP9045875.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3768 a         2719 c         2301 g         4245 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
     1261 aacggcaaaa tagttcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
     1321 ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg
     1381 tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat
     1441 tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct
     1501 ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca
     1561 ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg
     1621 atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg
     1681 ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt
     1741 ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata
     1801 taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact
     1861 aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct
     1921 ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc
     1981 tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg
     2041 cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc
     2101 ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc
     2161 taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat
     2221 ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat
     2281 ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt
     2341 caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt
     2401 cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc
     2461 cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct
     2521 gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata
     2581 atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg
     2641 ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta
     2701 tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact
     2761 tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat
     2821 tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact
     2881 ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt
     2941 ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct
     3001 tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt
     3061 ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc
     3121 tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct
     3181 gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct
     3241 gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc
     3301 cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca
     3361 tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct
     3421 gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt
     3481 ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct
     3541 ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta
     3601 tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact
     3661 ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt
     3721 ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac
     3781 gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga
     3841 tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc
     3901 gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag
     3961 aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag
     4021 cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt
     4081 tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc
     4141 catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt
     4201 cataaggagt caattccaat gcttcataga tatgatacat atctggatgg atcttttggg
     4261 acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg
     4321 aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat
     4381 aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga
     4441 ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta
     4501 attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat
     4561 gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga
     4621 aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact
     4681 ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca
     4741 aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta
     4801 gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc
     4861 atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct
     4921 aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag
     4981 aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca
     5041 gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat
     5101 atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag
     5161 cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg
     5221 ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat
     5281 gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg
     5341 attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat
     5401 tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc
     5461 cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat
     5521 gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga
     5581 gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga
     5641 cttttattat attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata
     5701 tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg
     5761 agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc
     5821 tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta
     5881 ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca
     5941 tctttttaga tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg
     6001 acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca
     6061 cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat
     6121 cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat
     6181 tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc
     6241 tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa
     6301 gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga
     6361 taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc
     6421 ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga
     6481 gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg
     6541 cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa
     6601 taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt
     6661 tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa
     6721 ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac tctcttagct
     6781 tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg
     6841 acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag
     6901 aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc
     6961 tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt
     7021 ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc
     7081 ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac
     7141 tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc
     7201 ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt
     7261 gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat
     7321 ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat
     7381 gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct
     7441 aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct
     7501 ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg
     7561 tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc
     7621 gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag
     7681 tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt attaacataa
     7741 aaatcacgtg atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg
     7801 ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat
     7861 tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa
     7921 aaaaacagaa agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt
     7981 aagaaattat attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat
     8041 tgaaagacta gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac
     8101 acactacatt tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc
     8161 tcatcagcta aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg
     8221 ccaggtcatg actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg
     8281 taccacgctt ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat
     8341 tttaggtatt ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt
     8401 aagtatcggt gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa
     8461 agaatggaat cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat
     8521 cgcaatcatc agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac
     8581 agatgatcct cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat
     8641 catcagtttc ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca
     8701 aatggtcctt tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa
     8761 cagctctgtt tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca
     8821 aaaagaacgt gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc
     8881 ataaaaaaac tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     8941 tataaacggc ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
     9001 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     9061 ctatttttta tagtaattcc ttcagatact ttcttgcctc atcattgaaa cgcagtgatt
     9121 cttctaactt ctgataatat tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt
     9181 ttcctttcca tttgtcgatt gtctcttgca catttccaga ttggatatct ttcactacta
     9241 gttgccattt ttcttttgag actgtacggt ctttcttatc agcagattgt atagcttctc
     9301 gttctttacg caatactttt aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga
     9361 acccttctgt aaaaccatag cgacaatatt gcttccaaat aaatagatag atttttggtt
     9421 ggatgaatcg ttggtttttc gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt
     9481 gacgttttgt ctttctcaac acggaaataa atgtttcaaa ataagcatta ggatgtactg
     9541 gcacttcttc atatacgatg gattgccaat ctttacgtaa aaatgcgcga tagttgatca
     9601 agaattcttc tacatccttt actaattctt ctgcaaagtc gtcaaatagt tcttttttta
     9661 tctgtcgctt ttttgactgc ccattgatgt gttcacttat tttcttgatt tcattttcca
     9721 atgcttgata atccgctatg taaatcggca tttctacttg gcgaaattct gcgagccgct
     9781 gagctaattt tatatccggt aaatatggct gtttcatccg acgattttgg tacaagacaa
     9841 aacagtattt ttgccatagt ccttcaggaa aatcgatatt cttctgctta agccataata
     9901 attcgtctaa ggccagatca acttttgtca ttccctgatg gatcgaagct gccactgtcg
     9961 gagagacatg caggtgatcg gcaaagtggt tgatgcctag ttttaggatc tttccggttt
    10021 ttggcgattt aacgatgtat tgcactttta attctctacc acactcacaa tacaattttt
    10081 gctggcttcg ttttgttcga aaaaacggat tgatcttgaa gtcagaaaac acccaatctg
    10141 ttgcagctaa ataatgttct tccataaaac gagagctgat tagatatcgg cggtgcttat
    10201 cgactagttc ccgctgtttt ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg
    10261 tgtattgttt agaaattttg atgggatagc ttttgctgat ttcttttgga aaccattctt
    10321 caaagacgga ttgccattct tccacggctg tttctttcct ttctaattac aaaaagctgg
    10381 ctactaacat aacgttagca accagccagt gtttcttgct aaattagtga gttgttccgg
    10441 catttccgga aatatcttct ttattttcta ctgctgcttc aactgcataa gtcaaagaag
    10501 aagcaatgtc ttctaaagac atagaaggcg tacccactgg acgatcgatc acttgatcgg
    10561 gtgagaatgg cacgtggatg aacccacctc gaattgccgg gaattgtgtt tcgatcaaat
    10621 gaagcaaacg atacatgata tcattgcaga caaatgttcc tgctgtataa gagatgtacg
    10681 ctggtagacc atgatcttgt acattttttg tcattgcttt gattggcaat gaggtaaagt
    10741 aagcggctgg accgttttct tctaaagccg tatcgaatgg ctgatttcct tcattatcag
    10801 ggattcttgc ttctgctaga ttgattgcga cacgttcgaa cgagactgct gaccgcccac
    10861 ctgcttgtcc gacacagatc acgacatctg gttggtgctg ttggatggct tctttgacta
    10921 cttgactgct tttttcgaat acggtcggta cttccacttt gatgatttca gcaccgctga
    10981 tttcatctgg catttttttt actgcttcat aggctggatt cactttgtcc ccaccaaatg
    11041 gatcaaaacc tgttactagt actttcatct gttgttcctc cgtccgattg atttaacata
    11101 tggttatctt ttatcatacc attttccttg aacagcgtaa ataaaggaaa caatagttta
    11161 aataacataa cagatcaaac ttaatatatt cttataaaaa aatagtgttc cactacaatg
    11221 ttagaagagg ttgcgacaat tattctgtca caacccctct cttctattat ttaaatagaa
    11281 tcatataagc aatttggaaa actagcataa ataacgcaat aaacaactgg tttttgatca
    11341 ctccgtattt gtctttcatc tccagcatcg ctacaggcac gatattgaag ttagcagcca
    11401 tcggtgtcag cagggtccca caaaaaccgc aagtcaatgc aaccattcct attaacgctg
    11461 gattcgctcc atgactaaat acgaatggtg caccgatccc aaccgtcatc acggtgatgg
    11521 ccgcaaatgc attccccatg attaccgtaa atatcaccat gccaagagcg taaatgatga
    11581 tgccgatgtt cacatttcct tctggaacga tcgtacctac tgcattggaa atcactgtcc
    11641 caactccagc gcttgtaaat accgcaccta gagaagctaa aagcattggc aacatgctta
    11701 acggccctac tgtgcccagc atatctgctg catcatctaa aaatgtactt ggtcgattat
    11761 ctcgagaaaa gaacatcaag attagcatgg ctgcaaaaac accgacgccc acgccgacta
    11821 aagcacctaa attggtaaat agagcaaaca agatcgcaaa gacaccgata ctcaaagctg
    11881 gaataaaaat tttcattcct agtttgtctg acattttttc catatattct tttgatggaa
    11941 gccggctttc tcctttgctg actttcttag caattgctgg tatggtcatc gcaaagatca
    12001 agccaccgtt cacgattgcc ggaagccatc ttcctcctgc aataactacc cctaagaccg
    12061 tccagaaaaa ggctgaccca taacgatgag gattggtttt atctgttaaa tttcttactc
    12121 cagtgtaaat caatacgagt cccatcacga tataaaggat ctccagtaat ttatcgccta
    12181 ataaaatatc aggatttgta aaaaagtcca tcttattgcc cctttcctgg ataatatttc
    12241 ttcgctaatt tcttttcttt aagatagaag tagacacttg ccacaactaa agccgtgata
    12301 gccactggaa cttccactaa agcaagctgt cccaatgtta cgtcatatcc tagatctttc
    12361 agtgttgatt ggactagcaa cgcccctgct cctccgatga atagtacttg gccaaagaac
    12421 cagcagacat tttccatagc tgaagccata ccttttagtt cttcttcatg cttaggatgg
    12481 acttctaacc cttttgattc gatggtcccc aatgccatcg gtaaaatgat tggacgaaca
    12541 aatccagcaa ctccaccaaa actcacgtta aaagccgcaa agatcattcg aaagatccca
    12601 taaatatcta caatcatacc agcagatact ttcttgaatc gcttgatcaa ggaagccgct
    12661 gattctttca gaccatttct ttcaagcgtt cctgttgcca acataatgat gataaaaatc
    12721 gccatcccgc gattgtttac aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca
    12781 agaccgccag tcaaagctgt aacaactaaa gcaaccatta cgatcaatat cgagtctaat
    12841 ttaaatgcaa acccgagaat aacaatcaag acgcctaaaa ggctaagata attttccata
    12901 tattttcctc cataaaatga caatattttt ttaattataa agcaaaacac ataaaaaata
    12961 tagttgtttt ctcacgaaat agacactatt tttaaatatt tttcgttatt tttttatcac
    13021 aaaaattatc tta
//
LOCUS       DAZKBH010000076        12979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000076 DAZKBH010000000
VERSION     DAZKBH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.076"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACG8OO_002013"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACG8OO_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9045876.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACG8OO_002014"
     CDS             complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACG8OO_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9045877.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="ACG8OO_002015"
     CDS             complement(3218..5533)
                     /locus_tag="ACG8OO_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9045878.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="ACG8OO_002016"
     CDS             complement(5718..6578)
                     /locus_tag="ACG8OO_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFP9045879.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7269)
                     /locus_tag="ACG8OO_002017"
                     /pseudo
     CDS             complement(6637..7269)
                     /locus_tag="ACG8OO_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7436..8197
                     /locus_tag="ACG8OO_002018"
     CDS             7436..8197
                     /locus_tag="ACG8OO_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9045880.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="ACG8OO_002019"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="ACG8OO_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9045881.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACG8OO_002020"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACG8OO_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9045882.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="ACG8OO_002021"
     CDS             12557..12913
                     /locus_tag="ACG8OO_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9045883.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3727 a         2714 c         2310 g         4228 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaaagaggct gggacagaag
    12961 cgtttaactc caagaaata
//
LOCUS       DAZKBH010000077        12818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000077 DAZKBH010000000
VERSION     DAZKBH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.077"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..310
                     /locus_tag="ACG8OO_002022"
     CDS             161..310
                     /locus_tag="ACG8OO_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFP9045884.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            321..1835
                     /gene="dltA"
                     /locus_tag="ACG8OO_002023"
     CDS             321..1835
                     /gene="dltA"
                     /locus_tag="ACG8OO_002023"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFP9045885.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1832..3037
                     /gene="dltB"
                     /locus_tag="ACG8OO_002024"
     CDS             1832..3037
                     /gene="dltB"
                     /locus_tag="ACG8OO_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFP9045886.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3082..3315
                     /gene="dltC"
                     /locus_tag="ACG8OO_002025"
     CDS             3082..3315
                     /gene="dltC"
                     /locus_tag="ACG8OO_002025"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFP9045887.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3318..4586
                     /gene="dltD"
                     /locus_tag="ACG8OO_002026"
     CDS             3318..4586
                     /gene="dltD"
                     /locus_tag="ACG8OO_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFP9045888.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4727..5809
                     /locus_tag="ACG8OO_002027"
     CDS             4727..5809
                     /locus_tag="ACG8OO_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFP9045889.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5809..6612
                     /locus_tag="ACG8OO_002028"
     CDS             5809..6612
                     /locus_tag="ACG8OO_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9045890.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6682..7422
                     /locus_tag="ACG8OO_002029"
     CDS             6682..7422
                     /locus_tag="ACG8OO_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9045891.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7433..8437
                     /locus_tag="ACG8OO_002030"
     CDS             7433..8437
                     /locus_tag="ACG8OO_002030"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFP9045892.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8482..10326)
                     /locus_tag="ACG8OO_002031"
     CDS             complement(8482..10326)
                     /locus_tag="ACG8OO_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045893.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            10538..11056
                     /locus_tag="ACG8OO_002032"
     CDS             10538..11056
                     /locus_tag="ACG8OO_002032"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9045894.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            11184..11867
                     /locus_tag="ACG8OO_002033"
     CDS             11184..11867
                     /locus_tag="ACG8OO_002033"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFP9045895.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            12176..12249
                     /locus_tag="ACG8OO_002034"
     tRNA            12176..12249
                     /locus_tag="ACG8OO_002034"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12210..12212,aa:Arg,seq:tct)
     gene            12408..12767
                     /locus_tag="ACG8OO_002035"
     CDS             12408..12767
                     /locus_tag="ACG8OO_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFP9045896.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT         4186 a         2069 c         2576 g         3987 t
ORIGIN      
        1 gagaaattat aaagaaaaaa gcagtgattt gatgtgctat ttttataaaa gctatgtgac
       61 aaaactgtta gtaaaaaaac ggtttctgta agctagacac atgtttcttt ttgtcaaaat
      121 tagttacact ttgtgtgagg ttatatgagg agggaatcca atgaacaaat ggcgttctaa
      181 tccaaaattg aattattggg gaaaattcat cattcgaaca attttttata gtgcagtact
      241 gctgttcctg atttatctgt atcactataa aaatattcaa ggaggaactt ttatctataa
      301 cgaattttag gaggaagagt atggaaatca aaacgattat tgaagcaatc gatgattggg
      361 gaataaatga acctgatcga gttgcttatc aggcggatga gacgcatact tttggtgaat
      421 tgaaacaagc atcagacgca cttgcttatt atttagaaaa aaaggtagaa ggcgatggac
      481 caattgtcgt ttttggcaat ttggagtttg aaatgattgt ctcatttcta ggggttgtca
      541 aatctggcca tgcctatttg ccgattgaag agcatacacc caaggagcgt atcctatcga
      601 tctttcgggt ggctaaacca tctatggtca tcagtatcgg ggactggata gaagatattc
      661 cttcggttcc tgtcataaca aaaaaagaat ttcaagaaat caagaagata ccagtaggat
      721 tttatcccag aaatagcgta aaaggtacag aaaattttta tattattttt acttctggta
      781 ctactggaga accaaaaggc gtacaaatca gtcatgataa tctagtcagc tttgttcaat
      841 ggacgatgga agactttggg atcaagcctg gtatgcattt cttagcccaa gcgccatttt
      901 cttttgattt atcggtattt tctatttatc ctgcattagt ttctggcgga atgctgaagc
      961 cgttaaataa agcagttgtt caagactttc gtcaattatt cgcgacattg ccagccttga
     1021 agttgaatgt gtgggtttct actccatcat ttatggatat ttgcttgatg gaacctacat
     1081 tcaatggaga aaatgtgccg gaattggaca tgtttttatt ttgcggagaa gaattgacga
     1141 aaaaaactgc agaaagtcta cttgatcgat tcccaaatgc acggatatat aatacttacg
     1201 gtccaacaga agcaactgta gctatttcaa gtattcagat tgaccagcat gtactagata
     1261 gttatgaccg actaccgatt ggctacgtaa aaaaagacac gaaagtttct attgtccaag
     1321 aaggtaaacc tgtttcaaaa ggagaaacgg gtgaaatcat tattgcagga ccaagcgtat
     1381 caaaaggtta cctgaacaat cccgaaaaga cagctgctgc gttcttttgt caaaatgact
     1441 tagcaagtta ccatacaggg gatgcaggta gactagatga atatgggctt ttattttatg
     1501 aaggacgtat ggatcagcag gtcaaattac atggataccg gattgaactc ggggatatcg
     1561 aacattatct gctccaagac aaccgaatca aacaagctgt cgtcgtacca aaataccaag
     1621 gaacaaaagt acagcagctg gttgcttttg tcgtattaga aaacaaaacg aaacagccgg
     1681 attttcagtt gactaaatct atcaaagaac aattactaga agttgtaatg gattatatga
     1741 ttcctcagaa gttccattat gtcgatctgc tgcctcagac agttaatgga aaaatcgatc
     1801 gaaaaaaatt aacagctgag gtgaatcctg aatgatgtta cctcatatga tcccttatgc
     1861 agagccgttt tatttttttc ttttagccat tgcgcttgta ccgatcgttc tatctttatt
     1921 gatatgggaa aaaagattac cagtttatca atcagcagtg acacttttct ttttatttgt
     1981 cagctttgga ggtaattatt ggcaacaggg agcggcgctg attgggtatg ttttgtggca
     2041 gacgatactt gtatggggct attttgttta tcggcaaagg aaaaatcaga caggatggtt
     2101 ttttgccgcc gttattttag cgataattcc actgttttta acgaaagtga ctcctttttt
     2161 tgaacatgga aaagcatcgt tacttggttt tttaggaatc tcttatttaa cctttaaatc
     2221 tgtacaaatg atcatggaaa taagagatgg catgatcaaa acatataagc catttcgtta
     2281 tatccagttt ctgctgttct ttccaacaat ctcttctgga ccaatcgatc gctataggag
     2341 atttgaaaaa gatcagctcc atccgcctgt tcgtgataaa tatgttgatc ttttgggaaa
     2401 aggcgtccaa aatatttttg tcggtttttt atataaattc attatcggtt attattttgg
     2461 tcaagtactg cttcctgtag tgggacgcat tgccttacag catggtggta tttcatgggc
     2521 attagtaggt tatatgtacg tgtacagtat gtatttgttt tttgattttg ctggctatag
     2581 cttatttgca gtcggtacaa gctacatgat gggatatcag acaccaatca atttcaataa
     2641 acctttttta tcatggaata tcaaagaatt ctggaatcgt tggcatatga cgctgtcctt
     2701 ttggttccgt gattatgtat atatgcgatt gatgttcttc atgatgaaga aaaaattttt
     2761 taaaagcaag atcgtaatgt caaatatcgg ttattttgct ttgtttcttc taatgggtat
     2821 atggcatgga ttgacgtggt actacatcgt atatggactg tatcatgctt gtctgatctg
     2881 tctgacagat gcatggatcc gatttaagaa gaagcataaa gagcagttgc cttctaacaa
     2941 atttacgcat ggcttagccg tatttttgac atttcaagca gtatgtttta gcttcctgat
     3001 tttttcagga tttttagata aacttttttt tagctaacga atagtgacat attaatttat
     3061 ataacaaagg agaaaaggaa aatggaagaa caaattttat caatcttaga agaaataaca
     3121 ggaacagatg aagtaagaaa aaatcgtgat attgatctat ttgatgaagg gttgcttgat
     3181 tctttagcat ctgttcaatt attagtcgag ctagacggag agctggatat ccaagtccct
     3241 gtatctgaat tcgagcgaga ggattggagc acaccgaata aaatcattca acaagcaact
     3301 gcattgaagg gttgagtatg aaaaagaaac tttttgctat tttcggcccg gttgttttag
     3361 ctgttggctt actgtttgtg ttttttacct cctcctatca agtagatatc tctaatccag
     3421 aaacgattag acgtgcctct tcttccatgt cagtgaacgt gttgaaagga gatgcgataa
     3481 aaaattatgc attatctgaa aaacaatata ttcctttttt tggctcaagt gaattaagca
     3541 gaatcagtcc atttcatccg tctgttctgg cagaaaaata ccagcgaaac tatcgtccat
     3601 ttttgctggg agcacccggg acacaatcgt taagccaata tatgatgatg cgttctgcag
     3661 gagatgctat gaaaaacaag aaagtcgtct ttattatttc tcctcagtgg ttcgttaaaa
     3721 atggtgtaaa aactgattac tttaatacgt attattctga acttcaaaca tacgactggt
     3781 tgttttcaat gaagaaagta acgcctgctg atcgctattt agctagaaga ttattaactt
     3841 tctcaaaagt aaaagagaat gatacgttga cggcgattct gcaaacgatc aaaaaaggga
     3901 agctaccttt acctgaatct ttgaatcagc tgcgcagcca atggaacatg ctgaagagag
     3961 aggatgaagt attcagcaat attgggttga aagatcgaca gcaaaaaatc gatcatgagt
     4021 caaaacgatt accaaaacaa taccaagaaa cagaattatc cattttagca aatcaaattg
     4081 gagaacgaga aacgacgaat aatccattcg ggttgaaaaa cgatttttat acgcatcgta
     4141 tccgagccca cgagccagag ctcaaacagt cacaaaaaaa ttgggattat cgtttttcgc
     4201 cagaattttc tgattttcag ctggtcttgg atcaattagc aaaaaaccat aacgaagtat
     4261 tgttcatcat cccaccggtt aacgagaaat ggagcgatta taccggacta tctcaagaaa
     4321 tgttacaagg atttgccaaa aagatcaaat ttcaattaaa tagccaagga ttcaatcgaa
     4381 tagctgattt tgtcaatcaa gcgggtacca actattttat ggaagacacg atccatctag
     4441 gctggaaagg ctggttggct gcagatcaac agatcagacc gtttttagaa gaaaatcata
     4501 ttactgctag taagtatcat cttgacgatg ctttttttag caaatcttgg caacatcaaa
     4561 taccagataa actacaatta aaataaagaa aacaggcgag tgttgctcgc ttgttttctt
     4621 tatttttgaa tattctgaat aaaagataaa aataccaagt ttgcctgaac aaaaatcata
     4681 agcaatggta taatcacaat atgaatatag aaatggggga aaacatttgg atacaaaaga
     4741 agaacagcta cttattgatt taacttctgc aaaaggagta cctggaaatg aagaagaggt
     4801 aagagaagta ttcagagaat atgccaaacc attcgctgat gatatttttt atgacggttt
     4861 aggcagtgtg attgccaaac atggtgctgg cggaggcccc aaggtcttta tttctggaca
     4921 tatggacgaa gttggtttta tggtaacaaa aataaccgaa aaaggattct tggaattcca
     4981 aactcttgga ggttggtggg ggcaagtcat gcttgctcag caagttgaaa tcaaaacacg
     5041 tgaagggaaa atcgtccacg gtgtgattgg ttccaaacca ccacatgttt taacacctca
     5101 agtaagaaat aaaccttatg aaataaagga tatgttcatt gatattggcg catcaagcca
     5161 agaagaagcc aaagagtggg gcatacgtcc tggagatatg gtcactccat atattgaata
     5221 caaacgaatg aatggcagta aatatttact ggcaaaagca tgggataatc gtatcgggac
     5281 agctgtttct ctgcgagtat tagagaatct atctaaagaa gcacacccaa atgttttatt
     5341 cgcaggaagc gatgtgcaag aagaagtagg cttgcgaggt gctcgaacaa gtacacacct
     5401 tgtaaatccc gatattgctt ttgctttaga tactggtaca gcaggagaca ctccaggaat
     5461 gacaccaaaa gaagcagatt cgattttagg aaaaggccca cagattttga tttttgatgc
     5521 atctatgatc ccgcataaaa aattattgaa ttttgttatt gatgtagcag aggaaatgga
     5581 tattccattc caatatacag tgatttctgg cggaggaact gatgcaggac aaatgcattt
     5641 gacaagagat ggtgttccgt ctgtagccat taccgttcct gttcgttatc tgcattccca
     5701 tacatcagtg attcatgaag atgattattt gaatacagtg aaactagtca cagaattagt
     5761 gaaacggctt gatagtgaaa cagttgaaaa aattcggagt tatgaataat gaataataga
     5821 atacctattt ctgaatatcg gaaaaaagca cgagtttctt tggaaaatca atggggaatc
     5881 aatacttggc ttgttttctt atctataata tttacgagcg tcatccagaa cttttttgac
     5941 ggtttcagtc cttttccgga agaaagcatg cagcagcact tattgagttt tctagtcgat
     6001 aatttacttt tattcgcatt caactatgca ttgttttaca ttgcgctagt agttgtacgg
     6061 ggtggcagac caaaaagtaa ccttttattt tcagttttcc aaagaaagta ttttggttcg
     6121 atacttggca tcaatttgat caattcagtc atcaactggc tactcaacag tttaatcttt
     6181 ctgcccatgt ttttaatcag cggagtgagt acttatatgc agctgctttt cagttcaaat
     6241 gcggtaccta cgggtgtcat gccagaagat tttcttgata tcagctttgt tttatcgatg
     6301 atagggatga tttttgtttc agcactcatc atgagtattg ttggcggttt attccaattt
     6361 gctgcatgga caaagttcga ctatcctgat ttgtcggtta ttcaatgttt gaaatatggc
     6421 tggtttttat tgaaagatag aattggtacg tatattttgt tgcagctgtc ttttatcggc
     6481 tggtatttgc taggagcaat cgctttgttc atcggtcttt tgtgggtcat tgtttatgtg
     6541 aatgtgtcag ttgcagaatt ttatgaacaa gcacggattg aaaaaggagg tccagacgcc
     6601 tattttactt agcatgtaag cagtttattc atcaaaaaaa taacttttgt ggtaggataa
     6661 atctttgagg gggaatgtac gatgaaatta actgttttag gatgcttagg agcgtatcct
     6721 tataaaaatc aagggacaac aggttatttg cttcaatctg acgggttcaa tttgttgatc
     6781 gatgcaggta gtgctacgct gatcaaatta caagaacacc ttgatccact tgatctagat
     6841 gctgtgatcc ttagccatta tcatcatgat catattgctg accttggtgt attacagtat
     6901 tactggcaac ttcatcctaa gagaaatccg gagacattgc tttcaattta tggacacaca
     6961 aaagattcca ttcactttga agaattgacg ttggaaaatg tctcaaaagg acatccttat
     7021 tttgaagctg aagaattaaa aattggtcct tttctgatta cttttatgga aacaatccat
     7081 ccagtagtat gttacgccat gcgtatcgta gaagaaacaa cggggaaagt attcgtattt
     7141 acaggtgatt caggttattt ggaaagtttc gctgattttg cgaaagatgc tgatttgttt
     7201 ctagcagata cctatctttt tgaaggaaat gaacatcatc atgcccattt cacttcaaaa
     7261 gaagctggag aacttgcgaa atcagctaac gtaaagaaac ttgtacttac acacctacct
     7321 cagtttggtg atttagaaca attgaaaaat gaagcgcaaa aggcagcagg caaagggatt
     7381 gatatagaac ttgctgcagt tgacaaagtg tttaacattt aggaggaaga acatgatttt
     7441 tgtaccaaat gaaaaccacg acccaagaat caatttagca attgaacaat atttgctgca
     7501 agaaatgcct ttagatgaac ctattttgct cttttatatc aatgaacctt caatcatcat
     7561 cggcagaaac caaaatacgc ttgaagaaat caatcgagat tatgtggaag aaaatggcat
     7621 tcatgttgtc cgtcgcttaa gtggaggcgg agctgtctac catgattttg gaaacctgaa
     7681 ctttagtttt atcatgcctg atgatgggga ttcattccgt gactttgcga aagtaacaca
     7741 gccaatcatc caagcacttc atgaattggg tgttgctggg gcggaattga aaggccgaaa
     7801 tgatctagtc attgatggca tgaaattttc tggcaacgcg atgtatgcta ccaacggtcg
     7861 gatgtttgct catggaacaa ttatgttcga ctctgatatc aatgaagtag tcaatgcatt
     7921 aaaagtaaaa aaagacaaaa tcgaatcaaa aggaatcaaa tcgatccgtt ctcgtgtaac
     7981 gaatatcaaa ccatttcttc cagaagaaaa gcaaaaaatg acaacagaag aatttcgtca
     8041 agaaatcttg ttgaaaattt tcggcgtaga gtcagtagaa gaagtaaaaa cgtacgaatt
     8101 gactgatgaa gattggaaaa aaatcaatcg aatctctgag gaatattatc gaaattggga
     8161 ttggaattac gggaagtctc cagatttcaa tttctctcgc caaaaacgtt tttctatcgg
     8221 ttcgattgaa gtccatttga atgtctcaga aggaatgatc aaagaagcaa aaatctttgg
     8281 cgatttcttc ggactaggag atatctctga cgtggaaaaa aaattggttg gacagaaata
     8341 tgataaagca tctctatcaa aaatagtcag ccaaattgat gtgaagaaat attttggtgc
     8401 gatagaacca gaagaattgc ttaccttact ttattgaaaa taaaaaatag cggaatccac
     8461 atatatggat tccgctattt tttatacagg attcgggtaa tttcgagaga gttgaacagt
     8521 gatccgatca atgaaagtat ctttttcctg cgttagtgtt cctgtgtaac gatccgctac
     8581 gtctttcatc atcattaagt caaaacgttc ctgatcatct aattcttctg tgatgcctcg
     8641 actaaatagg tttgtatcgt aatgtgcact atgttcgcaa caaatgacga aatgttcctc
     8701 ttgttcgtgt atcgacaaat aaatttttcg atacagtgga ttttcgatgg catgagtttc
     8761 tctaaaggaa tgctctaaag cgtggatcaa aagttgagcc agatcgtcat cgttgatagg
     8821 aagttcttcg ggaagatctg cttggaaggt tatctgaata ttccgtttgg cagctaactt
     8881 ctggtaacgc gctaagataa gatttgtcac ctggtggttg caaaaagaac acagcatttc
     8941 gaaatttttt gcttcttcta atatcttttc cacatagttt ttagcttttg tttggtccgt
     9001 atgcattaaa tcttgcagat tctccatctc ttggacaaat tccaggcgaa gtgtatttac
     9061 ttcttggata tgctgtcgaa gctgctcata atgttgctcc aacaaatcat ttttgatttt
     9121 taagaaatct acttgtctcc gatctttgtc ggtatttaag atgtgatcga ttagattgta
     9181 gctgataata acgattagaa tgataatata taaaatcgta gaccaattga tcgttgtttg
     9241 atagatacct aatgccgttt ggatgtaaat caagcaatgg ataatggcta ccaagacgat
     9301 ccaaggtgta caagctacat aaaaacggtt attcttatat gcttcgccaa tgcatgcaag
     9361 tgaagtgact aaagtgctta taacactagc aatattagca atcggcatta attcaggaag
     9421 ttctgcagga gtaaaagttg cataaagcag aacgccgata aaaatcaaaa gttggaacgt
     9481 acaaaaaatt ccgtaccatt ttttatagta aaacattcgg ctccaatagt agcagatgat
     9541 gaaagctgac gttaagataa taaataaatg agataaaaaa gaatgaacga atggttcaaa
     9601 aagaatacca cttaaaatat cttctgatac agcttcaaat cctaatgcca aagcaaagca
     9661 acttaagaga ataagtgagt attcaagacg tttttgttta tacatcaagt agatcgtata
     9721 gaaaaaggtc cctatagcga taaaaacagc gattatcatc gtcaatactt gcggcactga
     9781 ttgtgaataa ataaaactaa tcaatggtcc agttgttcct agataaactt tcggtggcag
     9841 tccagcatag ttctgataag gagatgagac tgtaagggtc aatttttttc cgatataatt
     9901 tgctggtaaa tctatcacag ccaatgagag tccgggattg ctttcttcct gataatcttt
     9961 ccgttggtat aaaatctttt cgcctagata tacagtaatc cattgatggt ttcctttgat
    10021 tagtaaattt gcttcgtcca acggtttttc aagtgtcgtt tccataatga atgtttcgtc
    10081 tttatccacg gtaggaatcg tacgagtgta cttgctgtca aacttaaaat caggatcatc
    10141 ttttgtatag aaaacccacg aatcttttat ttctttcatt tgattgctgt ccgcaggaga
    10201 aaaaataaaa taaaagaaat aagcaattcc tgttaaaaat aacaatgata atataatgat
    10261 tatgctccac tttatacgta aataaaaaga ttctttttta ttatatatgt tattaaaaaa
    10321 agacataaag gtattccttt ctaaaacttg taatgttagg gtaaacgttt tttaaactaa
    10381 cgtcaattat ttataataaa aaatgagata aagatgagga aatagaatta gaaacatata
    10441 attattaaag ggaaacattt ttacagctat aatttgaaaa aaatttatcg ttattatatg
    10501 attaagaagg attgaataaa gaaagaagtt gaaaacgata cctgattaca aaaaaataac
    10561 ttttataagt tctataacgt tgattatatt tttaattctt attcttctcg tcgtcttggt
    10621 tccccgaatt tttggtttga ctccttatat tattgaggac aaagaaatga gtccatatta
    10681 tgcacaagga aatctgatat atgtacgacc gaaaaataca aatgagattt tagtcggaga
    10741 tattattaca tattatgaaa attcaggaaa aaaaatctcg acgcgtcgcg taatagccgt
    10801 tgaaaacaac cacgaagatt tttatacaaa agctgataca aagacttata ttgagcctgg
    10861 agtagtaaaa aagagaaata ttatcggtag tcccatcttt cagattccat atattggact
    10921 agtcctttca aaaacggcat ttgcatggat tcaggggtta ttctttacga ttgctttttg
    10981 cctaacagga atcacagcat ggaatacctt tgtagagttg aaaaagaagc ggagacgaac
    11041 acagaaattt ttgtaagaat gttaaaaaca taaatgtatt ccagagatcc aacgactctt
    11101 ctgaacatga aacacgagaa aacactcatg cagcttgtag atttagtcag ttgttcgtat
    11161 aatgtgtgaa gaggaggagt agtatgcgca atgaaatgat gcatcgtcct gtagtcaaag
    11221 aagaactatt ggattttatg cgtacccagc aaaaaaaatt accgggtgag ctaggcaaac
    11281 tggaagaaga agctcatgta gcagaagtac cagttattcc ccatgaaaca gttgtatttt
    11341 tgaaattttt acttggacag cttcagcctg aacgaatctt ggagatcgga gcagctatcg
    11401 gtttttcatc aagtgtcatg gccacgacaa tcggagaaaa cgctcatgtt acgacaattg
    11461 atcgtttcga tgtcatgatc gagaaagcta aaaaaaacta tgaacgtcta ggactgacag
    11521 ataaagtgac tttgcttgaa ggtcaagcag cagatatctt gccggagttg tctggtccgt
    11581 atgactttat ttttatggat agtgcaaaat caaaatatat tgaattttta cctgaatgct
    11641 tacgtttgtt aagaaaaggt ggcgttttga tggtagatga catcttccaa ggaggaacga
    11701 tcttgctccc agatgaagag attccccgtg gcaaacgagc tatccatcgt aaattaaatg
    11761 aatttcttcg tgttgtgatg gatcatcccg atttgacatc gacaatcttg ccattaggtg
    11821 atggtgtgat cttaatgaca aaggaatcag aaacaatcga gttgtgatac atgattatgg
    11881 cagcctgtag ataatcgatt gaatcgaggg ctcaattcga acttgatgta aaagtttcta
    11941 tatttgtaag tcaattaaag gatacaggaa atctaaataa acaggagtag catgaagtca
    12001 atgtagctcc tgttttcaat tgataacctt agaacagctg aaggagaatg tctcgtttta
    12061 aaagattgat ttagtagttg ggccaaaata attcattatt tgctaattaa ataactattt
    12121 cgtattgaca gaagcaattt aattatctat aattattgat gttccgctga ttcttgtcat
    12181 agtagctcag ctggatagag catccgcctt ctaagcggac ggtcgggggt tcgaatccct
    12241 cctgtgacgc taaaaaaccg ttagaaactg aggtttctaa cggtttttct ttgttttttg
    12301 tgccaatatt gtttgttttc aaaggcaaat aaaataaaaa aacatcctta tcgagtacaa
    12361 ttataaggag caaaaaaata aaactcatca gaaaagagga agaagatatg acgatactat
    12421 ctagaatttt agtgactcta gttgcaattg aattttttta tatcatgtat atcgaaacgg
    12481 tgagaactga ttctgacacg acaagtaggg tttttaaaat gagtaaagaa gagttaagca
    12541 gaaaatctgt tcaaacattg tttaagaacc aaggagtgta caatggacta ttaggagtgg
    12601 gactacttta tggagcttat ctttcttccg catctaaaga aatcacaagt atgctattaa
    12661 tatctatttt ttttgttgca ttatatggca gtttgactag cgatcgttgg attttactca
    12721 aacaaggtgg gctagctctg ttggcattgc tttctatttt cttataaata tagaaaaaat
    12781 aagaatttat ctaacaggaa actttgtgaa aaatatta
//
LOCUS       DAZKBH010000078        12701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000078 DAZKBH010000000
VERSION     DAZKBH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.078"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1118)
                     /locus_tag="ACG8OO_002036"
     CDS             complement(<1..1118)
                     /locus_tag="ACG8OO_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9045897.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLE"
     gene            complement(1240..1836)
                     /locus_tag="ACG8OO_002037"
     CDS             complement(1240..1836)
                     /locus_tag="ACG8OO_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9045898.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2036..4678)
                     /gene="alaS"
                     /locus_tag="ACG8OO_002038"
     CDS             complement(2036..4678)
                     /gene="alaS"
                     /locus_tag="ACG8OO_002038"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9045899.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5066..5497
                     /locus_tag="ACG8OO_002039"
     CDS             5066..5497
                     /locus_tag="ACG8OO_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9045900.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5542..6891)
                     /locus_tag="ACG8OO_002040"
     CDS             complement(5542..6891)
                     /locus_tag="ACG8OO_002040"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9045901.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(6961..8124)
                     /locus_tag="ACG8OO_002041"
     CDS             complement(6961..8124)
                     /locus_tag="ACG8OO_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9045902.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8253..8576
                     /locus_tag="ACG8OO_002042"
     CDS             8253..8576
                     /locus_tag="ACG8OO_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045903.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8759..10108)
                     /locus_tag="ACG8OO_002043"
     CDS             complement(8759..10108)
                     /locus_tag="ACG8OO_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9045904.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10177..11130)
                     /locus_tag="ACG8OO_002044"
     CDS             complement(10177..11130)
                     /locus_tag="ACG8OO_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9045905.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11232..12422)
                     /locus_tag="ACG8OO_002045"
     CDS             complement(11232..12422)
                     /locus_tag="ACG8OO_002045"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9045906.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         3638 a         2685 c         2206 g         4172 t
ORIGIN      
        1 atttccaatt ggtaatctcc gattttttct tctatttgac tacgtaagat tctttctagt
       61 tctgtatcaa caaactggaa tttctcaggg accactacat caaattgcag cgtttttcct
      121 tgatgaatta ctctaaaatc atgaatatta agccctgtac ctgtttcaag aagaatttgc
      181 tttaactgcc gcaagatatt atagtatttt tcgttatcaa caggcattgg atccaagtga
      241 cagacaagtt ccacatccaa agacttttta aagtcttttt ctatcagatc aataatctta
      301 tgagccacat tcaaattcat tgattcatct acttcgatgt ggactgatgc aaagcgttta
      361 ttgggtccat agttatgcac caataaatca tgatagccaa gtatatcatc gcaagcattt
      421 aaatgctctt ccatttcttc aatctctgct ggcgttgggc gacttcctaa tagttcgtat
      481 acaaaatttc gaagcatcgt caatccacta taaaggatat ataatgctaa agcaaaacct
      541 atatatccat caatacgcca tccagttacc cattcgattg ctgctgaaag caatacagcc
      601 agtgtagtaa agacatcatt ataactatct gttgctgtag ctctcaatgt ttcagaatca
      661 atggattgcg caataacctg atacattttg ccttgtgcaa atttcaaaaa gatggaaatg
      721 attaggacaa aaaagacgat gggtgttaaa gagacatttt ctggatggat gattttttca
      781 gcagaagact ctaaaaattg aaatcctaca taagtaatga tgatcgaaac aaataaccca
      841 ctgatatatt cgaagcgttc gtgtccataa ggatgttcct gatctgctgg ctttgcagca
      901 attttaaatc cgatcaaagt caatatggat gaagcagtat ctgatagact gttcactgca
      961 tcagccataa tggagacact tcctgaaaaa agcccgatca ttagcttaga gacaaacaag
     1021 atcagattcg taaccaatcc taagataccc gctagtactc ctttttttgt ccgctgttgc
     1081 ggtatttgtt tctttttttc ttcttgtttc ttagccaagt tttttacccc ttctagtttg
     1141 cttgttgctc aggtaaaaag atggagagga cgattgtttt cctctcctct cttccttttt
     1201 ctatctatcg tgcgtcctat tactattaaa agatggtcat tattctggtt tcaacggtgt
     1261 tgctgcaaat aacgcttttg ctagtaatgg tgttatgatt cccgcaccta ttgcttctgg
     1321 tatcccattg attgctgcaa cccctgctaa ggttttaaat aaaagagcag agtctacacc
     1381 ataggcatct gccgttgcac ctgtataaaa taatcccatg aatcctaaca ctaagactgt
     1441 attgaccaat gaacccagta cccctgcaaa ggctgagctg atcgttattg atttattttt
     1501 ttgataaatc cagtggaaaa ccaagcctgc tactaagcca acaagtactc tgggaactac
     1561 cgagataatc ggattcgtga acactaatgt atcaaatggc gttgttggca tggcataggc
     1621 acgaacaatt gtagcgattc cccaaaacaa gccaatcacc atgccatctt ttgggcctaa
     1681 tactactgca gcaataatta ctgtgatatg gataattgtt aaactaatga atcctaatgg
     1741 aataaacccc agccaaggaa ccatcgcttg tacgatgatg attgctaaca agatcgcacg
     1801 taagactagt cgaaaggtct tatttctact attcattttt tcaccccttt ttatttatac
     1861 taatcgtaaa aatttctgtc actttcgtca actaaagacc atccgaatcc gaccatttga
     1921 ttagatgcgc catccaattg aaaaaagtat gagggcaaga cttgtttgtc ttcgccccca
     1981 taatcatgtt tcgttcaatg tttgaacttt tgtttttctg tcaaacgtaa acgccctatt
     2041 gttgcgcaac ccattctttc gcagctttaa gtgcatctgg aataccagca ggatttttcc
     2101 cgccagcttg tgccatgtct ggacgtccac caccgccacc gcctactttc ggtgcgattg
     2161 ctttaatcaa gtcgccagct tttagtccag cttcatttga ttctttcgtc attgccgcta
     2221 gcagactgac ttttccttct tgttcgtttg ctaaaacaag aatatctgaa atattttttt
     2281 gtttccattg gtcagctaat tgacgcagct gattcatatc tttcactttt acttgtgctg
     2341 caatgattgt ttttccattg atcgtttctg gatctttgaa tacatcacca gcttgttgat
     2401 tcgctaactt gctagctaat tgttcatttt ccttttgtag ctctcttact tgctgttgca
     2461 attgttccgc ttttgaaaca acttctttca attgaggtga tttaacaatt gttgctactt
     2521 gttttagttg tttttcttct tcttcaagta gttcgtacgc ttctttactt gttacagcct
     2581 cgattcgtcg cacgcctgca ccaattcctg attcagaaac gattttgaag attccgatgt
     2641 tttctgtatt tgctacatgt gtgcctccgc acagttcgat tgaccagccg ccaatattta
     2701 ctactcgaac gtcatgtccg tatttttcac caaacaaagc cattgcaccc atgtttttgg
     2761 ctgtatcgat atctgtttca attgtttcaa ctgggatagc ttcccagatt ttttcgttga
     2821 cgattctttc catttcagct aattcatctg aagtgatttg accaaagtgg gtaaagtcga
     2881 atcgtaaatg tccaggagcg actaaagaac cagcttgatt tgcatgctcg ccaagtactt
     2941 ctttcaatgc cttgtgcagt agatgggttg ctgtatggtt tttgatgatc cgattgcgca
     3001 tcgattgatc gatcaataga tgataggttt gtccttcact cattggtgct tttacttcta
     3061 cggtgtgcat aaattgacca ttaggcgctt tttgaacatc tgtaacaact gctacgattt
     3121 cgcctacttc attcatgatc catccttgat ccgctacttg cccacccatt tctgcataga
     3181 aaggtgtttg atcaaagatc atttgagctt caccttctgc gacttcattc aaaagagact
     3241 cacctttaac gatgaccaat aattgactat ctgattcttg ttctgtgtat ccaatgaatt
     3301 tactttctac tttgatatct gtcaataaag cagattgtac ccccattgat ttttcagtac
     3361 tgcgggcaga gcgagcacgt tcgcgttgtg catccatttc tttttcaaaa ccagcatgat
     3421 caactttcag tccttcatct tctgccacct cttccgttaa ttctaccggg aagccatatg
     3481 tgtcgtatag tttgaagatg tcttttcctg ctagagtcgt cccgttttcg ttttttactt
     3541 cttggatcac ttgatttaag atttccaaac cttcattgat tgtttcattg aaacgttctt
     3601 cttctgtacg gataactttt tcaataaatt ctttttgttg aagtacttca ggataataac
     3661 ttaccatgat ttccccaaca acagggacta aacgatataa gaatgcttgt tcaataccta
     3721 attttttccc atgcatgaca gcacgacgaa gcaaacggcg taagacatac cctctccctt
     3781 cattcgatgg caatgcaccg tcgccaatag caaatgaaag ggcgcgaata tgatctgcga
     3841 ttactttgta agaaatatct gttactgggt tttcaccata ttttacttgt ttgcttagct
     3901 tttctacggc gtggataata ggcataaaca agtctgtctc aaagttcgtt gacgcatctt
     3961 gaacgattga gaccatgcgt tccaatccca tccccgtatc aatgttttta tgaggaagtg
     4021 gttcaaatgt gttatcaggc ttatggttga attctgaaaa tactaagttc cagatttcta
     4081 aataacgttc attttctcct cctggatagt tttcaggatc gtcttctgct aaatcgttat
     4141 aagcttctcc acggtcatag aaaatttccg tatccggacc acatgggcct gcaccgatat
     4201 cccagaagtt gtcttctaca tcaataatgt gatctagtgg caggccaact tcttcatgcc
     4261 agatgcgttt tgcttctgta tcttttggat aaactgttac ataaagtttt tctggatcga
     4321 atgccatcca ttttggatca gtcaaaaatt cccatgccca gtggatggct tcatttttga
     4381 aatagtcacc aatcgaaaaa ttacccagca tctcaaacat cgtatggtgc cgtgctgttt
     4441 taccgacatt ttcgatatca tttgttcgaa tagatttttg ggcatttgtg attcttggat
     4501 tttctggcac aactgaacca tcaaaatatt ttttcaatgt tgcaacacca gagttgatcc
     4561 acaataaggt tggatcgttc acggggatca aagacgcact aggttcaatc gtatgtcctt
     4621 tagattggaa gaaatcaaga aacatttgtc gaacttgtgc actacttaat tgtttcatgt
     4681 cattacctgc tttctataaa ttaaatacaa aaaaacacca tcacagccaa ggacgaaaac
     4741 tcgcggtacc accttgattg caatgatgtg taatcattac ctcttaaact cgataacgct
     4801 gagctgcgtc ggtatttcta ccaaaataga aaaagggagc aatttgatta gtgactattc
     4861 ttttttcagc caagaaagaa ttttctgtga gtcttaaaaa acaaatcata tccttttcgt
     4921 acttaattag tatacgcatt ctattgatca ctgtcaatat ctcaattttt attttcattt
     4981 ccttttggtc atactccacg atttttggta cgataagaat aaaggaatca atacatctct
     5041 gctattcatg acaaggagga atatgatgaa aaaaatagtt ggagcggatg aatggatacg
     5101 cgcagctagc tgctttttga gatatcaagt ttttgtatta gaacaaggga ttcctgcaga
     5161 actagaattc gacggaaaag atatcccgca acaaacctat tatctatttc ttgatcaaaa
     5221 aagaccagta gctactttgc gttatgaaaa gaaaacacct acttgcttca agccggaacg
     5281 attttgcgta gcaaaagaat accgaggaaa agggttaggc ggtcagttgc ttgaagctgc
     5341 tgaaaaacgg gcggtttcag aaggttgtca gactagttat gtagtagctg aaatgacggc
     5401 tgtagatttt tatgaaaaac aagggtatca tacttgcaca gaacctttta ttgaagatga
     5461 agtactttgt atcggaatga aaaaaagtct agcttaaaaa caagagattg tgacacgcct
     5521 tttgtcacaa cctcttgttt tttatttatg tgaatttttc ttatttttac gagcagtacg
     5581 gctttgctgt ctgcgttcga ttttgcgttc ttttttattt ttttctgcaa ttgcccaatc
     5641 gatttttttc ttataaccag gcttgatctt tttctttttc ttcttcacaa gaccaatcag
     5701 cgtgggatct aatacatctt ttgatttttc tcgtttggca cgtctattac gatcgtatgt
     5761 tttgacgatt tccccgtttt tgatttcttt tggttcaaat gagacgccca atttttctat
     5821 cgcatcaatg gcttgctcat catttggcga atataacgta atggccgttc cttttaagcc
     5881 attacgtcca gtacgtccga cacgatggat aaagaagtct agatcttctg gaatttctgc
     5941 attaataaca tgagagacac cttcgatatc gatccctcta gcagctaaat cagtcgctac
     6001 aacatattga tactccagat tctgtacttg tcgcatcact cgttttctct ctcttggggt
     6061 gatgtctcca tgaatctttg ctactttcaa gccttggttt ttcaagtaat cggcaatttc
     6121 atccacatgc tgttttgtat tggcaaatac gattgctaaa taaggatgac caattgtcag
     6181 cagttgataa atcatttgat tctcatcttt accttttgtt gaaatcagcc aattttcaat
     6241 gtcttcagaa ataactgctt ttggtttgat ttcttcaatg attggatttt ccatgtattt
     6301 tcgtaagaat ggacgaagtt tttctggaat agtggcagaa aaaactaaaa actgcagctt
     6361 ttctggtaat cgacttgcaa tttggtctac ttcatttaaa aaacccatat ccaatgtcat
     6421 atccgcttca tcgacaacaa atgcaaatgc ggtgtgaatc ttcagcgctt gttcattcat
     6481 catgtctaat attctaccag gcgtgcctac tacaacctgt ggctgttggt gatgcagacg
     6541 atttaattga cgctgtttgt ctgtaccgcc gacaaaattc gagacacgga tctccggtgt
     6601 agaaaattta gcgatttgtg tagctgcttc atagatctgt gcagctagtt ccctacttgg
     6661 agctgtaata acaacttgta cttcctcttt ttctgcattg attttttcaa acaaaggcaa
     6721 caaaaacgta tgtgtttttc ctgtaccagt ttgagattgc ccaatgatat tttttccttt
     6781 tgcaattaaa ggaatcaaac gttcctggac ctctgtgggc tgtttgaagc cctttgcggt
     6841 taacgcttca ttgataaacg gtttgaagcg gaatatatca aaattattca ttcgtgcacc
     6901 tcttctcttt aaattatcac gactttggca ttataacaca gacaaagcgt ttttgctcag
     6961 ctaatttcga gaatcatcat cctttgtttg gccaatcgtg ctaacagtat atgtgtgact
     7021 agctttttca taaaacagaa tccgtttttt tctcaaaaat gatagcaaga tatttaaaat
     7081 aaaaatacct tgtagtaaca agacaccgag agaaatgaat actgcactca tctgcaacac
     7141 cgtattcgtt gtcgaattca tgatttgctg aagcaactgc cccaaccagc ttaaaccata
     7201 aaaaagaaag tacagatacc cgtagcggat catcaatgct tttaaagaaa ttcgttcgcg
     7261 gtcttcttcc accaaacgga tacgcacaat ttttttgcca agtgtccatc cattggttat
     7321 aaacggtaca atcataaagt atacccctac ttctaataaa agccaccaga tatgagaagt
     7381 aactgcccct gaatcgaacg caattttaaa gaaaatcgcc gctaccaaat taataagaga
     7441 taaaaatgtc cagtcgatca aataagcgac caagcgtcga gtcaatgaga cagtctttcc
     7501 tttttcataa gaaagttcat ccattcgtct tcttgtagga agcataaatg taaataacgg
     7561 cgtaacgaca aaaccaatga ttccacccaa tgtatttgtc actaaatcat tcacatcaaa
     7621 taagcggtat ggtcttgggt agataaaata aagtccactt agctgcgtca attcaaaaaa
     7681 tagagaaagg cagaaactag caatcatcgt tttcataaaa gaaagccgga aataatatct
     7741 taaataaatg ccaaatggta tgagtaataa aacattaaaa gctggttcca aaaatacact
     7801 ctgcttcatt gccggcaggt aggtactagg ttcagacaag accaataccg tgttgtctct
     7861 gaaatgttgc catgaagcaa ataaatgcag ctccatcttt ggccctgtca tttgggcaac
     7921 cgtctccttt gccggaagtg gtaagatcac caaaaaatac gcacacaata aataaaaaac
     7981 gaaagaatac aaaataactg cccttgttac tagaaatgtt ccatactttc gatattcata
     8041 aataaaaaaa acagatgaga tcgctaaggc taaaaatgga aaaacaatca gcgcaaaacg
     8101 aatcggttcg atataacttg ccatatgact cctccttgat tctttgtatc tctcttatct
     8161 tagcagtcct ttgctgtttc aagcaagtaa cgaggctatt atttctcatt tctttcaaag
     8221 taaagtaaga taaacacaaa gggggtgtcg agatgctgaa tatgtacttt gtttttggcg
     8281 tacctatttt tttactgttc ctttatgcta cgatcgctta tgtaagaaaa agaactacaa
     8341 ttcattattt aggatttatt ctcctgatta tttcgggttt catgctagtc ttcaatcttc
     8401 agacttggca gcaagcactt cttgaaatgg ataaaatgac tccacatgct ttgtctaaag
     8461 tgcttggcta ccctgtctat ctgatttggc ttcccatttt catttctggt tgcttggttt
     8521 tattaaatat ttatcgcggc gtacgccgaa tagttcagtt acgcaaatca aagtaaaaga
     8581 cagaaatcgg ttgagcccga tttctgtctt ttactttgct atttcatcgt agacaaaacg
     8641 acatttacgt gatcaaaatt aacgagtact tttgacattt cttccacgta ctcctcattt
     8701 aaataaaata ttttgtttgg cagagaagtg cagttctaat aaaattcgaa tgttttagct
     8761 atctacgttt taaatagaga taaatcggga taccagcaag tgtcaatact atccccatcg
     8821 aagctaaaaa ggtctgagtg ataagcgtgc ttgatacgat aaaagtccct ccaatgattg
     8881 ctactagtgg aataaacgga tacattggaa ctttgtatgg tcgaaacaaa tcaggttctt
     8941 ttttacgaag aagaatgaca ccaacaaaga ccatcgtata aaaaatccaa atgacaaaga
     9001 tcagcatatc cgttagtgta tcaaaaccac cgatcatcat catgccaata gcgatgacta
     9061 gctctaagat acctgcggct acaggaactt tagttttatc atgaagttta gcaaagagct
     9121 tgctaaaagg caggttgttt tcttttgcca taacataagg gagacgcatt ccagtaaggg
     9181 tatagccatt gatggttcca taaacagaaa tcaagatacc aactgtgacc agtcttccac
     9241 cgaagccgcc aaaaatcatt tttgcaacgt cacttgccgc attgctgttt cctgcgactc
     9301 cgtcgataga tgcggttctt aagaacaccg catttactaa taaataaacg atcatgattc
     9361 cgatgattcc taaagaaatt gcttttggca aatctttcgc cggctttttc aattctcccg
     9421 aaatatttcc tacgtgtatc caaccgtcgt atgcgaacat cgttgctagc aagcccgctc
     9481 ccaaggctga gaaaaatgaa agattttctc ctgcttggat cggaaacagt tggaagtcta
     9541 ctccttcttg tcggaacaaa ccgaaaatga cgatcacaaa caaaggaata agcttgcaga
     9601 caagcgtaat cgactgaaaa gcccctccag ctttagaacc taaaaaattg atcaacagga
     9661 tcgatacagc agctgtaaca gctacgggaa caatcattga ctgggaaaga ccaaatagat
     9721 tgacgaactg tgtaccgaaa atgatcgaca aggctgctac atttgctgga aaataaatca
     9781 cgacttgcgc ccagcccaat aaaaaagctg ctgtatttcc gtaaatccgt tcgatatatt
     9841 taatcatccc tcctgtttct ggaatagcag ctgccagttc agctcctgtt agtcctgcac
     9901 agacagaaat cataccaccg agaaaccaag aaaacatatg caaactggcg gatcctgtaa
     9961 cttctgctac gcttgcggct ttaaaaaata cccctgcacc gatcactgtg cccataacag
    10021 tcgataaagc agtaaaaaat cccatcgtac gttttaattc attttctatt tttacttctt
    10081 ttactggcgt ggcttgatat tcttccatcc cttgtcccct ttcataccat agccaatccc
    10141 aaaagtataa aaaacttttt ggatgtggtt cttgttttat accgtctcta ccaatttttc
    10201 tttcagtttt tccaacatat cctttgtcat cacatctaag tcgtatttcg gcgaaaagcc
    10261 ccattcctct tgcgcacaag aaacatctag tgaatttggc caagattctg ctattgactg
    10321 acgtacagga tccacatcat aatccatttc aaaatctggt atgatttttt gaatagatgc
    10381 tttgatctct tccgggtcaa aagacatcgc cgatatattg aaggcatttc gatgaaccaa
    10441 tcgatcagga tctgcattca tgagttttat aatggcatcg atcgcatctg gcatatacat
    10501 catatccatt gatgtacctt gatcaatgaa actagtgtac tttccttctt tgattgctga
    10561 ataataaata tcgactgcgt agtctgtcgt accgcctcct ggcaaggttg cataagaaat
    10621 caatccaggg aaacggacac cacgagtatc tacgccatat ttcacataat aataatcaca
    10681 tagtagttct ccagctacct tcgttacccc atacatggtt gtaggacgct gaagagtatc
    10741 ttgtggggta tgatcttttg gagtatttgg accaaacgcg ccgattgaac ttggcgtaaa
    10801 aaatttcagc tggaattctc tagcgacttc caatgcattg accagcccag tcacgttcaa
    10861 attccaggct aattgtggtt ttgcttctgc aacagctgac aaaagagcag ccaaatgaat
    10921 caatgtgtcc acttgatatt gttctgctat gctgcgcatt ttttcgtaat ccattacatc
    10981 taggatctca aagatatcgt ttttctcaat gacttcaggc atgcgtatat ctgtcgcaat
    11041 gacattttca ctgcctagtt cttgacgtag ccgtgtgatc aactctgaac caatttgtcc
    11101 taaacatcct gtcaccaata cttttttcat gatgatcctc cctgatcttt tttactacta
    11161 tcttcagatg attaacaata tatgcgtgaa catgttctct aattttaaat agacgaaaaa
    11221 actaaattta gttaaataat ttccatttct tttcctattt tttgataaac tgctactgct
    11281 tcatcaagca tttccctcgt atgttccgca gtcggcatat ttctgatcct gcctgtccca
    11341 agaggcacag ttggataaac gattggttta gcataaatcc catgttcgat cagcttctta
    11401 gagaaagtct gagtcacttt ttcgtctccc aaaatgactg gagtaatagg tgtttcagac
    11461 ataccgatgt tatagcctac ttttttcaat tcttctttaa aataattagc attttcccaa
    11521 actttttcga caagttccgg atgttcttgc atcaaagtaa tcgaagccaa tgcagctgca
    11581 gctgcacctg gagttagcga agtagaaaat aggaatggcc gacttctagc tttcaaccag
    11641 tcgattagtg ttttcgatcc agctacataa cccccaacaa cacctatagc tttcgataac
    11701 gtgcccattt gcatatcgat tttgtcgctt aatccaaaat gcttgacggt tcctgcacct
    11761 tttccagtta cgcctgagcc gtgcgcatca tcgacgtagg tgatcagtcc atatttttca
    11821 acaatcggaa taatttccgg caatcgtgca atgtctccat ccatggagaa aacaccatct
    11881 gtgatataca tgattttttt atatttctta ctttctatcg cttcctttgc ttttttctca
    11941 aggtctttca tatcttgatg tttaattcgg atgatttttg ctccggataa gcggcaaccg
    12001 tcaataatag atgcatggtt taattcatcc gataagatag catcttcttt tgtcattact
    12061 gcagagatag cgcccatatt gcagttgaat ccagattgaa aggcgattgc tgcttctgtc
    12121 cctttgaatt ctgcgatttt ttcttctaat tgctgatgga ttttcagcga accattgatc
    12181 gttcggactg ctcctgctcc tacaccataa gtttcggttg cttcaataca tgcttttttc
    12241 aattcttcgc gattggcaaa tccaagataa ttgttggatg ctagattgat catctttttt
    12301 ccgtctacga tgatacaagc accattttca ctttctagta tgtcgatcgt attgtataac
    12361 cccttttctt tcagttgatc caattctttc tctaaataca ttgttagttc tttggaagac
    12421 attgtctcca cctccatatt catctgtctt gcagaacata ccaaacatat atcgtgtcat
    12481 tcatctacaa agcaagtatg ctatattttt gataacgctt acactgatta tgagaattat
    12541 ttaaccacat tttttattta cagtatcttt ttactttcaa atgacttata tagtatgaac
    12601 aattaccttt tagataatga tctattttac tctacctggg cagataaata caacacaacc
    12661 attgatagaa gccctttcgt taaaatcaat tgtattctga a
//
LOCUS       DAZKBH010000079        12528 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000079 DAZKBH010000000
VERSION     DAZKBH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.079"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(395..1564)
                     /locus_tag="ACG8OO_002046"
     CDS             complement(395..1564)
                     /locus_tag="ACG8OO_002046"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9045907.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1768..2943
                     /gene="mltG"
                     /locus_tag="ACG8OO_002047"
     CDS             1768..2943
                     /gene="mltG"
                     /locus_tag="ACG8OO_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFP9045908.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            3079..3558
                     /gene="greA"
                     /locus_tag="ACG8OO_002048"
     CDS             3079..3558
                     /gene="greA"
                     /locus_tag="ACG8OO_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9045909.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3727..4458
                     /gene="liaF"
                     /locus_tag="ACG8OO_002049"
     CDS             3727..4458
                     /gene="liaF"
                     /locus_tag="ACG8OO_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFP9045910.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4455..5522
                     /gene="liaS"
                     /locus_tag="ACG8OO_002050"
     CDS             4455..5522
                     /gene="liaS"
                     /locus_tag="ACG8OO_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFP9045911.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5529..6161
                     /locus_tag="ACG8OO_002051"
     CDS             5529..6161
                     /locus_tag="ACG8OO_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9045912.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6301..6966
                     /locus_tag="ACG8OO_002052"
     CDS             6301..6966
                     /locus_tag="ACG8OO_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFP9045913.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6994..7287
                     /locus_tag="ACG8OO_002053"
     CDS             6994..7287
                     /locus_tag="ACG8OO_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFP9045914.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7355..8302)
                     /locus_tag="ACG8OO_002054"
     CDS             complement(7355..8302)
                     /locus_tag="ACG8OO_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9045915.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8480..9283)
                     /locus_tag="ACG8OO_002055"
     CDS             complement(8480..9283)
                     /locus_tag="ACG8OO_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFP9045916.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9429..10343
                     /locus_tag="ACG8OO_002056"
     CDS             9429..10343
                     /locus_tag="ACG8OO_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9045917.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10391..10861
                     /locus_tag="ACG8OO_002057"
     CDS             10391..10861
                     /locus_tag="ACG8OO_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFP9045918.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10946..11554
                     /locus_tag="ACG8OO_002058"
     CDS             10946..11554
                     /locus_tag="ACG8OO_002058"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFP9045919.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11688..11888
                     /locus_tag="ACG8OO_002059"
     CDS             11688..11888
                     /locus_tag="ACG8OO_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9045920.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         4222 a         1917 c         2504 g         3885 t
ORIGIN      
        1 taaataaagt aacaatcagg tattcataca catttttttc taaaatctca ttatttattt
       61 ctataatcac gctaattgtt cttctttttt attgcataaa attaaaaata agaacaagcc
      121 atgaatgatt tgctagcaca tattttagca atctaaagat actattttag atttaatagg
      181 ccctttgtca aataatgttg gtaaagagaa atcttagaat aatttggaca gaacagaaag
      241 aagaacacct ctttctgttc tgtttatttg gaattaaagt ctgtagcaaa aattgatttt
      301 tagatttgtc ccacactctt tcctacgttt agtcgagtac tttttttaag caaatgctat
      361 tcaaagaagt agtatttgct atcttttcat catatcagct atttgccaac ttagtagttc
      421 cttccatcag gtcatagatt tggtttctta gatcagcagt aggcagtttg atagttttgt
      481 cagcttttat attgctttcc atgatgatag catcctgtga atctttgcta aatatagcag
      541 atgtatcaaa agagtcgata tttccttgcg acaggatata attatctccg ctatataccc
      601 caccactata tggatgtcca ttcgcatttt cacgccataa tgagaagagt aagtctttgg
      661 aaactagttt tccagataaa acttgtgtaa acagcgtatt taaatcatag acggacatac
      721 tgatattacc agtccctaat tcgttatgga tctctgtttc agattctgtc agtggtttaa
      781 agtaatcatc ctctttagac atttgatagg atagtgcatg attggatgaa tttttgactt
      841 ggtcataaaa ttttgtatgc tgtaatccta aaggttcgat gatatcatcg gtcaataatt
      901 tttcatacgg ttgatttgtt aatttgatca aaattccagt caatatggta aagttcaatg
      961 aatcataatg agaggtctga taatctgtaa attttaagtg ctgaatcgca aattgaacga
     1021 cttcttcatc agtcatcggt atggttggag aagcagttcg gtgcaagcct gagcgcatat
     1081 acagcaagtc acggatagta atttgctggc tgccagcaat ttgtggatag aattgagcca
     1141 gcttagtatc taaagaaagg tgcccacgct cgacttgttt taaaatcaaa agagtagtca
     1201 atgctttttg aaccgaacca atctgataat aaagagaagg atcatttggt tgttgcgtga
     1261 gtttattcgc atagccatag cctttttcca tgatgatgcg attatttttc tttacgtaaa
     1321 ttgagccaat ataatcattt tttcttaata gttgatcgat tttttcaacg agctgttgtt
     1381 cattggtagt cagtatcaac tttttttgag ctgattgagc atgggcaact atctgagagt
     1441 gttcattatt gtaaaattgc catccaagaa tagttcctgt agagaacgca ctaaggctaa
     1501 acaacacgac aacgatcatt aaaaatgaat attttctttt tttagcatgt tttccttttt
     1561 tcataatagt ttatttagtc tcctttcttt tttacagaaa gaataaagct aaaatagctt
     1621 gtatcttgat aacatttatt ttaatcttat agtaatggat ttactactta catttttgat
     1681 agaatattta aataaaatca ttcacgccaa ttcaaatatc atgtataatg aataaagtta
     1741 cgaacttgat agtaaggagg agttgttttg tcaggaaaca aacaacaccc aagtaaagat
     1801 aaaaaagaag ccagtaaggc agctacagca gatataatcg acaagcggag aaatctgcgt
     1861 aaaaaagaag ataagattgt gaggaagatc attttagtga ttgctcttac attattgatt
     1921 attggcggat ttcttggatt taccgtgtat cgttatgtag atagcgggtt gaagccttta
     1981 gataaatcag atgaccaatt agtccaagtc gagatcccga gtggctcttc taacaagcag
     2041 attggtgaga ttttggagaa ggacaatatt attaaaagcg ggattgtttt caattattat
     2101 acaaaattta aaaatttaac tggctttcaa gctggctatt atcagttagc gcctaatatg
     2161 actcttgacg agattggcaa acagttgcaa gaaggcggta cttctgagcc aacaaaagtt
     2221 gcagatggga aaattgccat tccagaaggg tacgatatcg atcagattgc tgaacgggta
     2281 gcgaaggtaa ctggtaaaga caagaaagaa tttttagatt tagtgaatga tgagactttc
     2341 tttaacgagt tgcaccaaaa attcccagaa ctgttggaaa gtgcatctaa agctgagaac
     2401 acaaaatacc gtttggaagg ttacttattc ccagccacat atgattatta cacgagtact
     2461 tcgcttaagg atctagtgat tgaaatggtg aacaagacga atacggtcat gcaaaactat
     2521 tattcagcta tcaagcagaa aaatttgact gtacaagaag ttttaactct agcttctcta
     2581 gttgaaaaag aaggcgtaaa agaaaatgat cgtaaaaaca ttgcacaagt cttctttaac
     2641 cgaatcaaag caaatatgcc acttcagtca gatatctctg ttttatacgc attaggtgaa
     2701 cacaaagaat tagtgacgta tgaagataca gctgtagatt caccatataa tttgtatacc
     2761 aataccggct atggacctgg accttttgac aatccaagcg aagaggcaat caaggcagta
     2821 ttagaaccag cagaaaatga ctattattat ttcgtagcag atacgagcac tggaaatgtt
     2881 tatttcgcta aaacttatga agaacatatg gaattagtgg aaaaatatgt aaataattct
     2941 tagaaaaaca ggctctagac attgatcatt tttagaaaaa catttacatt tgaagaaaag
     3001 cgtggtaatc ttttttggac tatattcctc aaaagattac cttttgcggt attttatcga
     3061 aaaaaggaga cgttacacat ggtagagaaa gtttatccaa tgactctaga aggtaaagaa
     3121 aaattagaaa aagaattaga agaactaaaa acagtcaaac gcggagaaat cattgaacga
     3181 atcaaaattg cccgcggttt cggagatctt tccgaaaact ctgaatatga atcagcgaaa
     3241 gatgaacaag catttgttga aggaagaatc acaacaattg aaaatatgat tcgttttgct
     3301 caaatcatcg ataacgacgg tgtagatgca gatgaggtat caattggtaa aacagttaca
     3361 ttcatcgaac taccagatgg agaagaagaa gagtacacga ttgtcggaag cgctgaagca
     3421 gatccgtttt ctggcaaaat ttccaatgat tctcctattg cgcaagcatt gattggcaaa
     3481 cgtaaaaatg atgaagtaac tattgctact ccaggtggcg atatgttagt taaaatcgtc
     3541 aaagtagaac aagcgtagtc tgactgaaag atctagagag ttcatcttac tgatgataca
     3601 ctctagatct ttttctcttt gtgtatttta gatttcaaca aacgatctta catctaaagt
     3661 cctatagcca acagttaaaa aaataggtat gatgaaatga gaatgtgtca ggagggagaa
     3721 agagcgatga atagttcttg gcgttttttt gtggtagttg aagcactact gctgttattt
     3781 gctgtttggc aaatcgtaaa caatactgga cttttgcttt tagtgatatt cggtattttc
     3841 aatatatatc tagctatgcg caaacatcct cgtactaaat ttcaaaattt tcaattgata
     3901 ttaggaagtt tggtcatttt ttttagctta gtcaacagtc ctgctttatg gatgatggct
     3961 gtatttgccg ttttatttat tggtctgaaa ggtgtagaga tttcagggat agatttaacc
     4021 aaaaatgctt tttggcgaaa gaaacagatc atgatgatcc aaacagagca attaaaaaca
     4081 cataataatg agcggaaaag acagcaatta ttcggtaacc aacgtattgg aaacgacgtc
     4141 tatgaatggg acgatatcaa catcgccgtc atttctggtg atactatcat agatttgggc
     4201 aatacgcttt tgccaaaaga tgataatatt gtgattgtaa gaaaagggat aggcagaaca
     4261 cggatcttag tacctttggg cgtagccatc cgattagagc atgcgacatt agtaggaaat
     4321 gtcttgtttg aagaagagca attttctttg aaaaatgaac aaatcaaaat ctacagcagt
     4381 gattatgatg aaaaccctcg tagattgaag attattacta ataccttgat tggagatgtc
     4441 gaggtgatac gaatatgatg ggaaaaatat ccagagcgat gctagctgtt tattcgggga
     4501 ttgcggcttt ccttattatt ctattttcac ttttcactta tttttatgcc agcaatcaaa
     4561 gtcattggtg gggagaatta ctgcgtgcac gtttattata tgttccgctt atcttccatt
     4621 tgttagccat ttccttaggt gtaggattaa ttgtctttct attattatca cttattcaaa
     4681 agacaaaata cgggaaaatc gaagaaaagc tgcgtgcact ttcttctggc aattatgaat
     4741 ccaaattgtt gcttcttccg atcccaagtg catcggacga tttatacatc aaagatattg
     4801 ataaggaaat cactaagata aaagaaaaaa tgattgaagt atctagtgaa ttacaaattg
     4861 taacgagccg tccccaatat gtagatggac aaaccaaaga agaaatttta gaattagaaa
     4921 gacatcgatt agcccgtgag ctgcatgatt cagtttcgca acaattgttt gcagcaatga
     4981 tgatgatgtc ggcattgacg gagcaagcag aaaaaagcga gacaccagaa atgtttcgca
     5041 agcagttgaa aatggtagca gagatcatca acgcttccca gtctgagatg cgtgcgctgc
     5101 ttcttcatct acgtccagtc aatttagaag agaaaagttt aaagcaaggt atcgagcagt
     5161 tattgaagga attgcaaaat aaaatccaga tttcgctgaa atgggatgta gaagatgtaa
     5221 aactatctag ttccattgag gatcacttgt tccgaatcgt tcaagaattg ttatcaaata
     5281 cattaagaca tgctaaagcc aatgaattag aggtatattt gcacaaaata gacaataatc
     5341 ttttgttacg tattattgac gacggaacag ggtttaatat gaatgaaacg aaaacgggaa
     5401 gttatggatt gaacaatatc aaagaacgag tagctggtat tggtggaaca gtaaaaatca
     5461 ttagttttaa aggtcaaggt acaagtgtag aaatcaaggt ccctttgatg aaggaggcat
     5521 agataaggat gataaaagtt ttattagtag atgaccatga aatggtgcgc ttaggcgtct
     5581 cttcttattt atctattcaa gaagacattg aagtaattgg agaagctgaa aatggacgac
     5641 aaggctatga gaaggcgatg acacttcgac cggacgtcat tttaatggac ttagtcatgg
     5701 aagaaatgga tggtatcgaa tcaacaaaag caatcttaaa agattggcca gaagctaaaa
     5761 tcattatcgt aaccagtttt attgatgacg aaaaagttta tcctgccatt gaagcaggtg
     5821 cagcagggta tttgttgaag acctctaccg cacatgaaat tgctgatgct ataagggcaa
     5881 cgcaacgtgg cgagcgagta ttagaaccag aagtgacaac aaaaatgatg gaaaaaatga
     5941 gtcggcgaaa tgagcctgta ttacatgaag aactaacgaa tcgggaaaat gaaattttaa
     6001 tgctgatttc tgaaggaaaa agtaatcagg aaatagcgga cgaattgttt attacactga
     6061 aaacagtaaa aacacatgtt tccaatatat tggctaagct agaagtggaa gaccgtacac
     6121 aagccgctat ctatgcattc aaacatggtt tggtgaaata aaaacgatga taaagaggat
     6181 aatcaaaagc tgagaaaata actattattt ttcttggctt ttttacaata atgatgcttg
     6241 cttttgtttt tgtaaaggct ttgttatact gagaaaagaa gatactaaag gaggaaaacg
     6301 gtgaaacaaa attttgcaat tgtaggtctt ggacgatttg gcggaagtat ctgccgaaca
     6361 ctgatcgaag ctggtcaaga agtactggct attgatagta atgaagatcg ggtcaatgaa
     6421 tatatgaata tagccactca tgctgttgtg gcaaatgcac aggatgaaat gacacttcga
     6481 tcattaggca taagaaattt cgatcatgta attgtcgcta ttggagaaga tattcaagcc
     6541 agtatcttag ttactctgat ggtaaaggaa atgggcgtac caaatatttt agcaaaagcc
     6601 caaaatgagt atcatgccag agtacttgaa aagatcggag ctgatcgcgt cgtccatccc
     6661 gagcgagaca tgggaataag gattgcgcat aatttagtgt caaagaatat tttagattac
     6721 ctagagttgt cagataaata ttctttagca gaaatccgag tctccaatcc taaatttttc
     6781 aacaaaacat tagctgaatt aaatttcagg caacgttttg acttaacagt agtcgccata
     6841 cgaagagtag atcaaacggt tatcgcttct cctgcagcag atgaatatgt tcgtgaaaat
     6901 gataatctac tggttattgg agagacaggc gatgtggata ttctagacga taagatgaat
     6961 caatagaaag aaaagagaaa gtaggttgtt gggatgaaat taacggtaac gccaaaagca
     7021 caaaagtggt ttgactcaga aattgatctg ccagaaactt acggaatacg tttttttgga
     7081 aaagtctacg gcaaaacaga ggttcatgat ggtttttcga ttggaatgtc agttgaacag
     7141 ccagaacatc ctgtaaagaa agtggaaatg gatgggacac ttttttttat cgaggaagca
     7201 gatgaatggt tctttaaagg atatgattta cttgttgatt atgatcccaa cctggaggag
     7261 ccaacctatc actttaaaaa acaataaaaa aagagagggt gacacaattt ttgtcacctt
     7321 ctcttttttc ttttgaaaat attgttttca gctattaata tcctgcagac aattttactt
     7381 gattggtttg aagctcatgg gtggcaaagt agcttttcag attggttaaa aaaatagcca
     7441 ttaatttctt ttgaaaatca ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt
     7501 ccattttcca aagaggatga tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg
     7561 caaaggaaag ttgctggtta tttaaagcct gcaccagatc ttgtgtctta acagaagcac
     7621 cacggccaac attgatgaat acagattttg gatcaaagtg ttcaaacata tgctgatcaa
     7681 agagcccagt tgtttcctcc gttagaggta agatattgac tacgaaatct acttcaggaa
     7741 gaacttcctt tagttgattt aaagaaaaag tcttttccat tccttctgct ggatgaccgg
     7801 atgtattgat tccatagcag ttcatgtcta atgctttgca tttttttgca atcatttttc
     7861 caatatggcc agttccaatg attagtattt ttttcccttg gatagattgg taatggaccg
     7921 atgaacctaa ccatttcatg tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat
     7981 acccaaggat gattcctaag acatgctccg taatcgattc actgtggatg ccactaccat
     8041 ttgaaagtaa aatatgtttc tttgaaaaag tctcaagggg tagctgatca acaccagctg
     8101 agatagattg gacccatttc aaggtagaag aggctaatag tttttcttgg tagtctgagt
     8161 tccagcccaa actgatctct acattttgta gatcctctgg ttgtaattct gttttaaaga
     8221 tataaccagg cgctatagta tggatttctt cgataaattc atcacgaaat ggacgatctg
     8281 cataaatgat tctttctgac atagttaatt tcccgccttt ctttactcta agtgtagcgc
     8341 atacaagaag aactttgaag cgccttgctt gttttttgag aaagaaatat ttttcaaata
     8401 aaaaaggggc tgtgacaaaa aaatgtcaca acctctaaaa gggtctattg aagtagaaat
     8461 aacgagttgt ctttatttct taattgatcg atatttttgc ccatcctttg acagcttgag
     8521 caaaaataaa gattcctgca aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga
     8581 ccatcaagat tgttgcaatt atagtaggca atgaaattga atagatcaat gtttttaaac
     8641 aatcaagaaa agtagctttt gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg
     8701 taaatagcaa agtgatcagc agattcagaa aactaggata aatagaaaat ataaatgtca
     8761 ggacttttat ccaaagtggt atagatgctt cacttaaaaa agaacggagc tgcttacctg
     8821 ttaagttttt taaagattca ttggtgtagg gaagttctaa ttggttattg ttcaacaaag
     8881 cagaagtcgt gcctgtatta ggaagagcta tgataagttt gttttttaac atcccgacac
     8941 taacaaaatt ccccatcaaa tcagtagaga tgtcctgttc tgttcgtttt ccctctggat
     9001 caaatgtgaa gataatggag tttgtttggt aaatgaatcc ttctttttct tcggtttgaa
     9061 gctgcccgtt ttggattgta aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt
     9121 gaatgttgtt taagacttga aaaacttgaa aagagattgg caaagccata attatactca
     9181 aaactaaaag atagacaatg gctttccaaa atgggacatt tctcgcctcc tttaggtctt
     9241 tgaatcgtat aaaggaactg ataattaatt gttttgttgt cataagtact cctcatctag
     9301 tagtgtcatc acttgcaatt ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa
     9361 cagctgaatg attggtgaaa tatcaaaaaa atgttattct tgtctaaaga ctatccaata
     9421 taaataaaat ggaggaagga atagtgccta cacagccaca gagatcatca aaaaaactat
     9481 ttggagtagt ggttttgaat gcggtgatca ctttatcaga atttattgga gggattatgt
     9541 cagggagttt gtctcttgtt tcagatgctt tccataacct tagtgataca ttggcaatta
     9601 ttttttctta cggggcacaa aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg
     9661 gctaccagcg tttagaaata ttgagtgctt tcatcaactc attcatattg atcatattat
     9721 ccttgttttt aaccgttgaa gcattcaagc ggttcacttc accagaaaag ataaatagtc
     9781 atttgatgct aacagtagca gttattggat tgctcgcgaa tttgttctca gtattattat
     9841 tacgacaaga agcagatgaa agtttgaata taaaatctag ttatttgcat ttgttaagtg
     9901 atacactgtc ttcggtaagt gtgattatcg gagcaatttt gatccgtttt ttcgatatct
     9961 actggatcga tccagttatt acactgatca tttcgatata tatattgata gaagcgataa
    10021 tggtgatcaa aaaagctgcc gctattttga tacaaagtgc acctgctatc gactatgaaa
    10081 aaatggaaca agaaattgaa gcaatcgatg gggtaaagga tgttcatcat gtccatattt
    10141 ggcagtatag cgaaaagata attattttcg acggacatat tgattttgaa gatcagctac
    10201 taagcgagat tgaaaagata tatcctcgta taatttcttt attaaaatta aaatatggta
    10261 ttacacatgt gacgatccaa gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt
    10321 taaataaaga tgaaagttat tgaaatatag agtatgataa ataagaattt ttaataaggt
    10381 aggttggctc atgaagcagt atcgacagtt aaaaggatat ttagaagcaa aaggatattg
    10441 ggagatacta gtctatctct tttttggtgg tttggctacg attgtaaatt ttgttagttt
    10501 tgctctcgca cgccaatatt ttgatttaag tatggcctta tccaattcta tttcgtggtt
    10561 ttgttctgta ttgtttgctt ttgtaacgaa caaactttgg gtattccatt caaaatcacc
    10621 gaattttaca catgcactta ttgaatgcgg taagttcttc ttctaccgta tcctttctta
    10681 cggattggac atgggggcaa tggtattgtt gatcaatgtt atgaacagca atgagtatgt
    10741 ggcgaaaatc atcactcaaa ttattgttat ccttgcgaac tatattttca gcaagttgtt
    10801 tatttttaaa gaaactgagg tttttgaaga agaagtaggt caaagtgaaa aagacgacta
    10861 ggcataacaa ttgaagaatt tccttctctt tgttactctt tgtaatgtag acattattaa
    10921 gaaaacattg aggaggaatt tttttatgac ttacacatta ccagatctac cttatgcata
    10981 tgacgcattg gaaccttata ttgacgaaga aacgatgcat ctgcatcatg ataagcatca
    11041 caatacttat gtgacgaatt taaattcagc aattgagaaa tacccagaat taggcgaaaa
    11101 aacaatagaa gaattattat ctgatatgga cgctattcca acagatatca agacagctgt
    11161 acgtaacaat ggtggcggac atgctaacca ttcatttttc tgggaaatca tggcaccaaa
    11221 tgctggtggc gaacctacag gagaaataaa agaagcgatt aatgaagctt ttggtgattt
    11281 ttcttctttt aaagaagaat tcaaaaaagc agccgctgga cgatttggtt ctggatgggc
    11341 ttggcttgta atggaaaatg gaaaattagc tattacctct actgcaaatc aagattctcc
    11401 attgatggaa ggaaagacac caattctagg tttggacgtt tgggaacatg cttattattt
    11461 gaaatataaa aatgtccgac cagattatat tgcagcattc tggaatgtta ttaattggga
    11521 tgaagtaaac aaacgtttcg aagcagcaaa ataaccattt cctccgtcat atcagtctta
    11581 aaaataattt ggaaaataaa agagcgaaag cttgctagcg ttttctaaat tgggtataat
    11641 gttcttgcaa ggttacttgc aagaactttt ttggaggata tacatttatg gaacacggaa
    11701 cggttaaatg gttcaacaat gaaaaaggat ttgggtttat tacagtagac ggcggagatg
    11761 acgtatttgt tcatttctca gcaattcaag gagacggctt caagagccta gaagaaggtc
    11821 aagaagtaga attcacgatt gttgaaggtg cacgcggacc tcaagctgct gaagtgacta
    11881 aattataata atttgttgaa taaagacacg acttcttaga gagtcgtgtc tttttgtcta
    11941 tttatgaagt ttttagattt tttggctaag taaatgcatt tgttcacttg acatgtccta
    12001 actaattatc aagcaaggaa cattttcttc gttagtaatg gattttaaag aaatttcagt
    12061 ttttggatgt tgaataaaag accatttgtg aaaccactag tatacatact cattaccatg
    12121 tcaattctat tactttaaaa gaaaatcgtt ttactttgaa gaggagcata tctatccatt
    12181 aaaagatgat ggtaatgcaa tcactagttc gagtcatttt aagagccatg cagtgagctg
    12241 gaaataaaac gaatagaaga tgggtttaac aaaaggacca gtcataggaa gaaattgtcg
    12301 tgaaatcgaa tgataatctg aacggtaggc aaagactgtt aaaaagatgg gaaaggtatg
    12361 tgcgatcatt ttactgcacg attatttgga aaataccttg ttgttcgctt tttctggaaa
    12421 aaatacataa aaaaagataa aacgattgcg aactgcgtgt attcggtctt tcgatctttt
    12481 tcacggtatg gtatgatagt taagaatttt gagaaatttt aaaaaata
//
LOCUS       DAZKBH010000080        12171 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000080 DAZKBH010000000
VERSION     DAZKBH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.080"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..297)
                     /locus_tag="ACG8OO_002060"
     CDS             complement(133..297)
                     /locus_tag="ACG8OO_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045921.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            592..1920
                     /locus_tag="ACG8OO_002061"
     CDS             592..1920
                     /locus_tag="ACG8OO_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFP9045922.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="ACG8OO_002062"
     CDS             2122..2955
                     /locus_tag="ACG8OO_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9045923.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="ACG8OO_002063"
     CDS             2971..3708
                     /locus_tag="ACG8OO_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFP9045924.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="ACG8OO_002064"
     CDS             complement(3863..4831)
                     /locus_tag="ACG8OO_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9045925.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="ACG8OO_002065"
     CDS             5063..5896
                     /locus_tag="ACG8OO_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9045926.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="ACG8OO_002066"
     CDS             5930..6769
                     /locus_tag="ACG8OO_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9045927.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="ACG8OO_002067"
     CDS             6795..7355
                     /locus_tag="ACG8OO_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9045928.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="ACG8OO_002068"
     CDS             7554..9275
                     /locus_tag="ACG8OO_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFP9045929.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="ACG8OO_002069"
     CDS             9440..10582
                     /locus_tag="ACG8OO_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9045930.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="ACG8OO_002070"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="ACG8OO_002070"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP9045931.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4148 a         2042 c         2527 g         3454 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DAZKBH010000081        11975 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000081 DAZKBH010000000
VERSION     DAZKBH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.081"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(358..843)
                     /locus_tag="ACG8OO_002071"
     CDS             complement(358..843)
                     /locus_tag="ACG8OO_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFP9045932.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    933..1278
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1323..1964
                     /locus_tag="ACG8OO_002072"
     CDS             1323..1964
                     /locus_tag="ACG8OO_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9045933.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1983..2618
                     /locus_tag="ACG8OO_002073"
     CDS             1983..2618
                     /locus_tag="ACG8OO_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9045934.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2636..3451
                     /locus_tag="ACG8OO_002074"
     CDS             2636..3451
                     /locus_tag="ACG8OO_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9045935.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3770..5206
                     /locus_tag="ACG8OO_002075"
     CDS             3770..5206
                     /locus_tag="ACG8OO_002075"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9045936.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5395..7842
                     /locus_tag="ACG8OO_002076"
     CDS             5395..7842
                     /locus_tag="ACG8OO_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9045937.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            8072..9139
                     /gene="rlmN"
                     /locus_tag="ACG8OO_002077"
     CDS             8072..9139
                     /gene="rlmN"
                     /locus_tag="ACG8OO_002077"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP9045938.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9129..9335
                     /locus_tag="ACG8OO_002078"
     CDS             9129..9335
                     /locus_tag="ACG8OO_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045939.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9332..10435)
                     /locus_tag="ACG8OO_002079"
     CDS             complement(9332..10435)
                     /locus_tag="ACG8OO_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9045940.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10988..11536
                     /gene="aac(6')"
                     /locus_tag="ACG8OO_002080"
     CDS             10988..11536
                     /gene="aac(6')"
                     /locus_tag="ACG8OO_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9045941.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(11784..11903)
                     /locus_tag="ACG8OO_002081"
     CDS             complement(11784..11903)
                     /locus_tag="ACG8OO_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9045942.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         3984 a         2001 c         2556 g         3434 t
ORIGIN      
        1 aaaaatctag ttttgctgta atcattggtt tattgatagt aactatctgt gctgggatat
       61 tcttacaaaa atataaaaaa gtttagttag aatataatat tttttgttaa atatgactga
      121 aatcaaaaga ataaaattcc gatttataat cgaaaaaaga agcattttta tatatgcttc
      181 ttttttcgta gaatcaataa atatggattt ggctaactat ggaagaatac tcgtattagt
      241 tttcagcgtt catactagtt gttacattgt ttgattggcc aaaagaattt ctgatatatt
      301 ttgtgctaaa taaagaaaaa gacgcttgaa ttgggaaata agaaaactac tctatcatta
      361 caaattttca taaactggca gatgttctaa gtaaacaagt atcttttcaa agtcggaaaa
      421 atccataatc aacgtagtgg tattgatatt tggatgaaag cctacggtat cttgacgatc
      481 aatagcagca tcgatcacga cttggatttt tttctctttg tcgtgcatca atgctaatgg
      541 agtcactgta cctgctggaa cgcctaggag gtcaggaagc tgttcttcag aaacaaaaga
      601 taagcgcttt tcttttaatt gttcagctag ttgttttaaa tcggcgtgct tttcatcagg
      661 caagatgaca agatagattt gttttccttt tcttgatttc aataatagat tttttacttg
      721 cggaccaggt agggaaaaag ggacattttt gactgacgta atcgcaggat gatcgacttt
      781 ttgaaaagag atacccagtt tttttagtag ttcataagct tcttgttcgg ttgcacgact
      841 catcgcgcat caccctcctt tttttattag tgtatcataa aaaagagttg acaaatgaaa
      901 aataatcatg taaattacac gtaaagttaa aacaaatgac atcgaaaaga agagtagccg
      961 ttaggtgttt ttagagagtc tctggttggt ggaaagaggc agcagttaac cgtgaaacac
     1021 atcttggagt cgattttctg aagcgtatag taggaaaatc cggggagccc gttacagctg
     1081 cggtcgttgc tagatgacca gataaggctg gcttttccgt gaggagtcgg taaaaaaagg
     1141 tggaaccacg tgattaacgt cctttgatct tcggatcaaa ggactttttg ttttcatctg
     1201 aacggcaacg actgtgtgag gttttttctg caaggaaaaa ataaacagag gtggaaccac
     1261 gtataatacg tcctcttggc atttgccaag aggatttttt tattttaaag gaggaaaaga
     1321 agatggagta tttgattgaa gttatgccgc aattattagt aggagcactc acaacattga
     1381 aattattttt atttaccttg attggctcat taccattagg gattgtttta gcattaggat
     1441 tgtctttgca ttttaagccg ctacgctact tgcttcaaac atatgtatgg gttatgcggg
     1501 gaacaccgct gttgttgcaa ctgattttta ttttttatgg gttgccgacg attgggatcg
     1561 tttttgatcg tttcgaagca gcactcattg cctttatctt aaactatgca gcttattttg
     1621 cagagatctt tcgtggaggg atacaatcga ttcctcaagg tcaatatgaa gcagcgcaag
     1681 ttttgcaatt aagtcccttt caaacggtga cgaaaatcgt catcccacaa gtgacaaaga
     1741 ttgttttacc ttctgtaggg aatgaagtta tcaacttggt caaagattct tccttgatct
     1801 atatattagg tttaggtgac gtaatgcgtg ctgggaaaat cgcgatggaa cgcgacgcga
     1861 ctttactgcc tttgctaggc gtggcaatca tctatcttac attgactggt attctaacaa
     1921 tcataatgaa acaagttgaa ggaaaattaa attattaccg ctaactgata ggaggaagaa
     1981 atatgattga attgaagaaa gtgaacaaag catttggtga aaaaaaaata atcaaagatt
     2041 tgaatctaat cattccagat gggaaaacat tagctatcgt aggtccttcc ggtggcggta
     2101 aaaccacgct tttacgtatc ttggctggct tagaaagttc tgacagcgga gaattttatt
     2161 tagacggtgt accgttcaac ccattcgagc ttaaaatgaa agaacaagtt gtaggagtcg
     2221 tattccaaga cttccagcta tttccacatc tgaatatttt tgaaaacatt accttagcac
     2281 caaaaatggt attgaaacag acaaaagaag aatacaccaa aaaagcagaa gaattagcgg
     2341 cttcattagg gattagtgat ttattgaata tctatcctta tcagctttct ggcggacaaa
     2401 aacagcgggt agcgattgcc agagcattgg ctatgaatcc acgggtattg gcttatgatg
     2461 aaccaacaag tgctttagat cctgaattac gccagcaagt cgagcgtttg atcctatcat
     2521 tgaaagaaga tggggtaacc caaattattg tcacccatga tttagtattt gcagaaaatg
     2581 tcggtgatca attactaaaa gtttccccaa ttcattaaga aaggagaaga tagagatgaa
     2641 aaaaaccagt tttctattcg ctttgatcgc aggtcttctt ctttttgcag gctgttcgaa
     2701 caaaaaaact tcagcagata attgggcgac tatcaacgaa gagaaaaaaa tcgttatagg
     2761 tttagacgat acatttgttc ctatgggatt tagagataaa gacgggaagc tcacaggatt
     2821 tgatattgat ttagccaaag cagtctttga acaatatggg attacggtag actttcagcc
     2881 cattgactgg tcaatgaagg aatttgaact gaacaatgga acgatcgatt tgatatggaa
     2941 cggctattcc aaaacgccgg caagagaaaa gaaagtgcaa tttacaaaac cttatatgga
     3001 aaacgatcaa gtgctgatca cacctaaaga atcaggaatc acaaactttg gacaaatgaa
     3061 taataagaga ttaggtgcgc aaaatgggtc atctggttat gatgtattta cgaaacaacc
     3121 agaagtatta aaagagatag tcaaaaacca agatgctgtt ttgtatgata gttttaatga
     3181 agcattgatc gatctgaaaa gcagacgggt cgatggtctg ctgatggata aagtatacgc
     3241 agactattat ttgaaacaaa gaaacgaact agaagacttc aacataacaa aaggttctta
     3301 tgagagtgag gattttgcag ttggtgcacg caagtcagat agtacattag tgaaaaaaat
     3361 caatgaagcc ctagatcaac tagagaaaac aggtgaattc caaaaaatat ccgataaatg
     3421 gtttggcgaa gatgttacgc cgagaaatta acaaataggg gactgtgaca aaagacgtgt
     3481 cacagtccct ttttccgaat aaacagtgtt ctcctgccaa ctccttgtga ctcggagctg
     3541 aatggcaggc tcacaacctc ttggtaaacg gtgttcaaaa gccagttttt cggtattaag
     3601 ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac tactcgaaac
     3661 taagcgactt tttcacaacc tcctttacat aattttattt tgttgtggaa ggggtttcgt
     3721 gttattctaa aagaggaacg attgaaggtt ataggaaagg aagagatgaa tggaacacaa
     3781 agaacttacc tcatttccaa aagattttct gtggggctct gcttctgcag cgtatcaagt
     3841 agaaggagct tggcaagaag atgggaaagg agaatcagtc tgggatcgat ttgtacggat
     3901 acctggaaag acatttaaag gaacgaacgg tgatttggca gtagaccact atcatcgtta
     3961 taaagaagat attgctttaa tgaaagaaca aggtctgaaa gcttaccgct tctctgtagc
     4021 ttggacacgg atttttccta atggacgagg agaagtcaat caagctgggc tcgcttttta
     4081 tgagagattg atcgacgaat tgattgaaaa cgatattgaa cctatgttga ctttatatca
     4141 ttgggatctt ccacaagcat tgcaagatga atataatggt tgggaatcac gtcagatcat
     4201 tgaagatttc actcattatg cagaaacatt atttgaggca ttccgcggga aagtccatta
     4261 ctggattagt ttgaatgaac aaaatatctt cacttcatta ggttatttgt tagcagcaca
     4321 tccgccaggt gtgacagacc cgaaacgaat gtatgaagtt aaccatattg ctaatttagc
     4381 aaatgcatct gtcatcaata aatttcacga gttagaaatg cctggaaaaa tcggaccaag
     4441 ctttgcgtat acgccaaatt atccaattga cagtaatcct gaaaatgttc tggctgctga
     4501 aaatgcagaa gacctgatgg ccaattattg gatggatgtc tatatgtggg gaaaatatcc
     4561 gattgctgcc atgaggttct tagaagaaaa aggatgggca ccaacgattg aagctggcga
     4621 tgcagaattg ctagaatctg ctaaaccaga ttttcttggg atcaattact atcaaacggc
     4681 gacgaatgca ttcaatccat taaacggagt tggtgctgga aaaatgaata cgacaggaaa
     4741 aaaaggttca tcagaagaaa ctggagttcc tggaatgtat aaaaaagttg agaatccatt
     4801 tgtcgaacga acaaattggg actgggagat tgacccagaa ggattaagga tcggactgcg
     4861 tcgtatcaca agccgttacc gcatgccggt actgattacg gaaaatggct taggagagta
     4921 tgacaagttg acagaagata aacaaatcca tgacgactat cgaatcaatt acttacaaag
     4981 ccacgtcaaa gccatcaaag aagcaatttc ggatggagca gaagtcctag gctattgcac
     5041 ctggtcttat acagatttgc ttagctggct gaacggttat caaaagcgat atggatttgt
     5101 atatgttgat caagacgaaa cacaagaagg atctttggaa cgatataaga aagacagtta
     5161 ttactggtac caaaaactga ttgaagaaaa tggagagaac ctttagttca catacttaaa
     5221 acaagaaggt acggctggat atggccgtgc cttttcttga taaaatcatt tagaaattaa
     5281 gaaaaactgt aaaataagct gaaaaatgaa agaaagcgtc agttctttca aacaaaactt
     5341 tagttccctt actttttttt gtataatgaa acttgatgaa atgagggata ataaatggca
     5401 caaaagaaac ggcctactaa aaaaaggaaa aagacgaaaa aaacgaaaaa gcaaaaacag
     5461 cagcaggaac aaatgctgac gattggtgtt gggtttgcct tgattttatt tgctgtcttc
     5521 ggctttttaa aattaggttt tttaggaata ttgattgcaa acggctttcg gatcattgca
     5581 ggaaatactt accaaatcct ctgtctttta ttagccgtat taggattttg gattgtcatt
     5641 aagaatacag agttttcaat tggtaaaaac cgacgctggt ttggcggcat actcttttat
     5701 ttcggtatct tattattgtt acatgcccat ttatttggca aactccatac tggggaacca
     5761 aatatcatgg ggacgacatg ggatttgtta gccagtgata taaaacaaag tcaagtagac
     5821 aataacgttg gtggtgggat gattggagca atcctttatc attttactta cttcttgatt
     5881 gcacagcctg gaagttattt agcagcagtg ctattactgg caggaggagc attcctgttc
     5941 agtaatctgg aaggctatca gctgttaaat ggtatccaat caattggaga acgagtacag
     6001 gaattattgg aaggtgatcc tgccaagcaa gctcgaaagc aagcggcaaa ggaagagaga
     6061 atgaaacagc gcgcagaagc gaaagaagca aggcgcttag ctgcaaaaga agctgcagaa
     6121 aaagaagctg cagaatatga gaagaaaaaa gcggtgagcc agcaaaggga aaatactgct
     6181 gatgaatggc aggaaccaga aaatacagag ccggagcagc taagttttgt tccaatcgac
     6241 agttttcaag aaaatatcca tcctgcgaat ctagagaaac cagtaccaga tacaccaaaa
     6301 cagaccaata cagcagaagg tttttcagat gagttgccag aggatgacgg tacctcatta
     6361 gaatttgaga ttgaagcaga acaggaaaat caagactatg aattaccgac agttgatttg
     6421 cttgattcta taccaactgt ggatcaaagt gatgagtata aaaaaattga aaaaaatatc
     6481 ggcgtattag aacaaacttt ccaaagtttc ggtgtcgatg ccaaggtcgt caaagccagc
     6541 ttaggaccat ccgtcacaaa attcgaagtc cagccggctg taggggtaaa agtaagtaaa
     6601 attgtgaact taacagatga tatcgcgctt gctttagcgg cgaaagatgt ccggatggaa
     6661 gcaccgattc caggtaaatc actgattggt attgaagtac caaatggaaa gatcagtatg
     6721 gtttccttta gagaaatcat cgaagcacaa ccaaaccacc cagataaatt actagaagta
     6781 cctttaggac gtgatgtttc tggacgggta caaacggctg atctttcgaa gatgcctcac
     6841 ttactcgtgg ctggttcgac tggtagtggg aagtcagtag caattaatgg gattatcaca
     6901 agtatattaa tgcgtgctaa gccccatgaa gtaaaactga tgatgatcga tccgaaaatg
     6961 gttgaattga atatgtacaa cggcatacct catctgttga cgccggtcgt gacgaaccct
     7021 agaaaagcag cgcaagcctt acaaaaagta gtacaagaaa tggaagaacg atatgaaaaa
     7081 ttcgcggcaa ccggagtaag aaatatttct ggttacaatg aatttgttca acagaaaaac
     7141 ttggaaaacg gaacgaaaca tccaacctta ccattcatcg tagtgatcgt agatgaatta
     7201 gctgatttga tgatggtagc cagcaatgaa gttgaagatg cgattattcg tttagcgcaa
     7261 atggctcggg cagcggggat tcatatgatc ctagctactc aacgtccaag tgtggatgtt
     7321 atcacaggaa tcatcaaagc aaatgtacct tccagaatgg catttgcggt atcaagcggt
     7381 acagattctc ggacgatcat tgattctaat ggggcagaaa agctcttagg gcgaggagat
     7441 atgctattct tgccaatggg tgagaataaa ccgatccgtg tgcaaggagc attcatttct
     7501 gaccacgaag tagaacgggt cgttcaattt gttacagatc agcaagaagc tcattatgaa
     7561 gagaaaatga tgccgacaga tgaagttgaa acgacaggtg ctccagaaca gccacaagat
     7621 gagctgtttg aagaagcaaa agcacttgtc gttgaaatgc aaacggcaag tatttccctt
     7681 ctccaaagaa gatttcggat cggttacaac cgagcagcac gtttagtaga tgaactggaa
     7741 gctcatggag ttgttggccc gtcagaagga agcaagcctc ggaaagtatt cattgagcag
     7801 gaagaaacat cagaaacgtt gccggacgat aatgacatgt aaaacttgtg tgatgaaggg
     7861 ttgaaaaacg aataaccaat aaaaagacaa ggcatgatga taccaaaaga aaaaatggtt
     7921 tttttccatc ttttctaata cggtatgatg tcttgtcttt ttagtttttg tgagattaaa
     7981 gttctattcg atttatttgt gaagtattgc attaaggaaa tgaatcatgt atagtatgta
     8041 gcgaaacttt gagaaaaaga gggaaaatac tttggaaaaa cagtccattt atggcttgac
     8101 gaatgaagaa ttaatcaatt ggtttataga aaacggcgag aaaaaattcc gagctgccca
     8161 agtttgggaa tggttgtatc aaaaacgtgt aagtaatttc acagaaatga cgaatctatc
     8221 gaaacaattg atcgaaaaat tatcggagca ttttatcatc aatcctctaa aacagatggt
     8281 cgtccaagaa gcttcggatg gtacagtaaa atatttgttt gaactccctg ataaaaacat
     8341 gattgagact gtactgatga gacaagaata tggcttgtca gtttgtgtga cgacacaagt
     8401 aggatgtaat atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct
     8461 gactgctgga gaaattgttg cacagatcat gatggtccaa cattattttg atgagcgcaa
     8521 tcttggcgaa cgcgtttcac atgtcgttgt aatggggatc ggtgagccat ttgataatta
     8581 cgacaatgta atggactttt tgcatatcat caatgatgcc aaaggattgg caattggtgc
     8641 tcggcacatt acagtttcca ctagtggatt agcacacaaa atcaaggaat tcgctaataa
     8701 cggcttgcaa gtcaatctgg ctatttcctt gcacgcgccg aataatgagg tgcgaacatc
     8761 gatcatgcgg atcaaccgaa gtttcccaat tgagaaattg atggaagctg tggatgagta
     8821 tttagagaaa acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc aagtaaatga
     8881 tagaccagag catgcgcaac aattagcaga tctattaaaa gataagaaaa aattagcata
     8941 tgtcaacttg attccatata atccagtcag tgaacatgat caatattctc gcagtccaaa
     9001 agctgatgta ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg
     9061 taaagaacat ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa
     9121 aaaaacagtt gccaaataat cgtatcctac tcgttttttc aattaccata aaaggagcag
     9181 ttttcttaat taaggaactg ttcaacttaa ataagagaaa agattctata gcgaaagcta
     9241 taaagctttt tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc aatttgatga
     9301 cagcctgctt gatgggggtt ttagcaatta tttaactgat tttcttatat ccttgctggc
     9361 tttcttgtgt agcatgataa gtttctacgt caagaatggc ctgtttaaat ttttctcggg
     9421 tgatttcata aggcacatgt ttaatatcat ttgttgccat ggctttatct aagattgctt
     9481 ctaaatcggt cgtgacattg agattcatgt cggttaacga tactggaagt tggttctttt
     9541 tcataaacac atagatttta tcaaacaaat cgaactgctg atccatcatc aataaggtca
     9601 gtacaccata actgacagat gctccgtgaa ggtatccctc cattttttct agaacagtcg
     9661 taccgtaata gaatgagtgt gccatagcag aattatagtt attgttaact aaaacagaaa
     9721 ccaatccggt agtaacaatg atatccgaaa cgacttgttc aattgcatat gagggacgat
     9781 cgtcggcact atctttcaat gcttgcgtac cataagtcaa gagacgatca atgcacattt
     9841 ttccgatttg gattcctaat ctatccgtgt agcctagatc atcgccacga gcagaaaaag
     9901 tagattcgac ttctttggat aaagcatcgc caatacctgc ccataagtat ttatcaggtg
     9961 cttcggcaat aattgctgta tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga
    10021 gtccgtccat tacgccttct tcgttataga tgacacatac cgcagtgacg ggcgaacagt
    10081 tcgaaccaat agtgggaaag gtgaagactg gtttagctag tttttcacca atgactttta
    10141 cagtatcgca agcacggcca ccaccgactg caaacaggac gtctgctttt tggacacttt
    10201 catgctggat caactgttca atatttccat aagttgaatt acccccgtac cagagactgg
    10261 cgacaacctc tatttcctgt gtcaaagcta cttttatttt gttttctgcc tttgaaagtg
    10321 cagtcttccc tccaatcagc actacttttg tcccatattc tttgacgatt ccatttgctt
    10381 tttcatacgc atctgttcca atggtataat ttggtaagaa aacactttga ctcatattca
    10441 tttccttctt tcttcgttga aacgggttaa aattttaaag agtgatcgat ctgactgcgg
    10501 ctgtagatgg agtagaggtg aacctaagac aggataagtg atgaataagg tggcatgaac
    10561 accttgaaat catcaataag tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac
    10621 aataatgaat ttttgttcct cttagtattt ctaccgattc cctttgcttt tttgtaatta
    10681 tctaaaaatt ctaaaaatta agattattat atgaaaacag gttttatttg tcaattcata
    10741 attggttttt tttaaagcaa aagataatct atatcatgat gaacaaacag agttttttcc
    10801 tccaattgat ggctttcctg atctttccta ttttgaataa agtgtttctg tataattttt
    10861 ttattttata agtaagataa ataatgaaag aatagagata ctgattggta gctaaagatt
    10921 tttgagcgag aacatttcgt tttttcaaaa ggactgttag aataagcaag aacaaggggg
    10981 aagacgtatg ataatcagtg aatttgaccg taataatcca gtattgaaag atcagctttc
    11041 tgatttactg agactgactt ggccggaaga atatggagac agctcggcag aagaagtaga
    11101 agaaatgatg aatccagaac gaatcgcggt agcagcggta gaccaagatg agttagtagg
    11161 atttattggt gcaatccctc aatacggtat cacaggttgg gaattgcatc cattagttgt
    11221 agaaagctcc cgacgaaaga accaaatagg tactcgatta gtcaattact tagaaaaaga
    11281 agtagcttcc agaggaggaa tcacgattta tttaggtacg gatgatttag accatggaac
    11341 aacgttaagt caaacggacc tgtatgagca tacatttgat aaagtggctt ccatccagaa
    11401 ccttcgtgaa catccgtatg aattctatga aaaattaggt tataaaatcg taggtgtctt
    11461 accaaatgca aatggctggg acaaaccgga tatttggatg gcaaaaacga ttattcctcg
    11521 accaaattct caataataaa aagaggttgt gacagaagta atcagcttca agaaataaga
    11581 aggaatttac gaaaattact tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag
    11641 ctacttttgg aacaccgtgt accagaggtt gtgaaaaagt cgcttagttt cgagtagtaa
    11701 gaaaaatttt gtgaaaatgg ctttctacat ttttgcataa atttggctta ataccgagaa
    11761 actggctttt gaacaccgtt tattcagata agggactgtg acaagacttt tgtcacagtt
    11821 tctttttgtt tattcttttt tatctttgta tggcaataat agacaatata acgaaaatta
    11881 tttaatggct tttggaagac catggaaaac cttgtacaaa gctatttttt ctgaaaaatt
    11941 ccttgaaacc tcacgaaaaa atcattattt tatga
//
LOCUS       DAZKBH010000082        11669 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000082 DAZKBH010000000
VERSION     DAZKBH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.082"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..217)
                     /locus_tag="ACG8OO_002082"
     CDS             complement(38..217)
                     /locus_tag="ACG8OO_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045943.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(233..433)
                     /locus_tag="ACG8OO_002083"
     CDS             complement(233..433)
                     /locus_tag="ACG8OO_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045944.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(598..1305)
                     /locus_tag="ACG8OO_002084"
     CDS             complement(598..1305)
                     /locus_tag="ACG8OO_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045945.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1298..1735)
                     /locus_tag="ACG8OO_002085"
     CDS             complement(1298..1735)
                     /locus_tag="ACG8OO_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045946.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1750..2001)
                     /locus_tag="ACG8OO_002086"
     CDS             complement(1750..2001)
                     /locus_tag="ACG8OO_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045947.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2001..2207)
                     /locus_tag="ACG8OO_002087"
     CDS             complement(2001..2207)
                     /locus_tag="ACG8OO_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFP9045948.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2644..2904)
                     /locus_tag="ACG8OO_002088"
     CDS             complement(2644..2904)
                     /locus_tag="ACG8OO_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045949.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3078..3182
                     /locus_tag="ACG8OO_002089"
     CDS             3078..3182
                     /locus_tag="ACG8OO_002089"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFP9045950.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3323..3808)
                     /gene="ssb"
                     /locus_tag="ACG8OO_002090"
     CDS             complement(3323..3808)
                     /gene="ssb"
                     /locus_tag="ACG8OO_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9045951.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3811..4710)
                     /locus_tag="ACG8OO_002091"
     CDS             complement(3811..4710)
                     /locus_tag="ACG8OO_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFP9045952.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4723..5316)
                     /locus_tag="ACG8OO_002092"
     CDS             complement(4723..5316)
                     /locus_tag="ACG8OO_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFP9045953.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5392..5541)
                     /locus_tag="ACG8OO_002093"
     CDS             complement(5392..5541)
                     /locus_tag="ACG8OO_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045954.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5557..7761)
                     /locus_tag="ACG8OO_002094"
     CDS             complement(5557..7761)
                     /locus_tag="ACG8OO_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFP9045955.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7772..9232)
                     /locus_tag="ACG8OO_002095"
     CDS             complement(7772..9232)
                     /locus_tag="ACG8OO_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP9045956.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9293..11665)
                     /locus_tag="ACG8OO_002096"
     CDS             complement(9293..11665)
                     /locus_tag="ACG8OO_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9045957.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2433 c         1958 g         4386 t
ORIGIN      
        1 gtgctatcac ttttagtttt taggataaaa agtgacgtca ctttttgagt gctggtacct
       61 caaatcctgc tttttcttgg gcaatcaata aatctaacgc aatacacata atcctggatt
      121 cacttttgcc gtacttctgc tttagttgct gcaattcttt ttgtctcttt tcatccaacg
      181 tcacgggtaa ccgtttattt cctattgcaa ttggcattag tactctctcc tttcatcgaa
      241 ataaccctat taaaaatatg gctgcaatca acttgaatat tagactcaca agggttaaca
      301 caaagcgcca aaagatcaaa caaaatacca gtattcctaa gaatactacg aagtttaaat
      361 gtagagctgc accatgacgc ataagtatct cgccaacaaa cagtacgaac aatagaggaa
      421 tgattttatt catatggatc tccttctttc tcttaggtta tcgggctttg cccgcttatt
      481 tcgcaccacc taagcggcgg gggcggttgg atcgattggt gcgaaataag aaccattccc
      541 ttaatttcaa ggttttttgt tcttatttca cacatcctca cttttcggta taaatggtta
      601 ttgacctttt ttcctttcaa atcgttgata tggcattcgt ttacggtcaa gttcattttg
      661 gatttcttct gtatcgtcgt aagtgtaggt gtctaaaaaa tcagttaaag tcgtgtacca
      721 gtcgtcgcct gggttatcta cttcttctac atattccctc aaaaatgctt cgggtacagt
      781 gagttcataa tccattccca ttaacacgat agattccgtc ccacacgtca aaaatctagt
      841 aaatacaagg taataatcgt ctaccgtcgc atctttgctg tttagctctt ccatttcttc
      901 ttccattaat tccatgctct caaaaattga ataatattct gattctgagt aatagtgttt
      961 cctgcaaatc gtgtagctga caaagttttc gaccagctca ctcctttcta ggtaaacatt
     1021 catgatatat ttttctgttt ctagtcgtag aagctccgct ccttttttta atgtgtcagg
     1081 acgacgttta tacacgtcta aacgattatg gctgactgta accttcgttt gactcgtttc
     1141 actttccata tacaactgtt cttccactct ttgaataaag tacttcattt cttccataca
     1201 actaaagtct ttttgaatcc gtctgattac ttcattcaag tgttctttgt ttgttaaaaa
     1261 ttgcgaaaca tcttctgttg ttagctgata aaccacctta ttcattatct ttctcctccg
     1321 attcaatcgt ataaaatgca ccattttttt cgatattttc aaaagtatct agccagtcgc
     1381 tagccattgc tttagctttc ttaatctgtg cgaattttgc ataagtcagt tctaaggctt
     1441 gatattcgga aggtttgata gtgcttggtt tgacacctac ttccaagcat agtaaggctt
     1501 ggaaaaacca ttttccattt tgttttttca gttcttccag tgttcgatag tacgtcgcaa
     1561 tgagattcat ttcgacacct gattcaatca tttcttccac tgcgactgac aatttttcct
     1621 tgtaatgttt tttgggtaca cgtgtttcat ggtataggaa cgtcatatcc tccatttttt
     1681 gtgccatttt atccaaaggt aatttgacaa attcgtcata gtaattttga gccatatttt
     1741 ttctcctttt tagttctcta atttggttaa ccaatattcg atttcataag tttccatgtt
     1801 ttcttgataa tgcagttcat atcctgcaat cttcactagg tcttcaaata gcatagtcgg
     1861 tgcatcctct actaaagggg tttctatcgt ccagtaccgc ggaataagta aagcttctgc
     1921 tactttttca gaaatgactt ctttgtccac caaaatttga acgacttctt ttacagtcaa
     1981 taatttgtgt tgtgttttca tcaccgtttc tttactttct catcaatcaa aacataatcg
     2041 ccataagaca aggtatattt tttcccgcat gttggacatt gatacaaacg atagcttttg
     2101 atacatttcg ctctttctcc acaatcgcat tcgacagtat gtggtgcatg atagtttggg
     2161 aataattcgc tgaagggaac accccaataa tcaaatttct ttcgcatgat tgattctgcc
     2221 tcctcttagg aaaggtttta tttcctttcg ttagttcttt ctttattgat ttgttggcca
     2281 gaatgaatca acgaaaagcg acaaggacaa gggcgcgcag cgtgccattt acccttgtcc
     2341 ttgtcgcttt ttggtgataa aaggcaactt gttgccggtc tggcaaatca ataaagagat
     2401 agcgaacagg aacgaacctt ttggtgagag gaagtttttt ctattttgtc ttgctttgca
     2461 agacaccata tcttagtgaa cgtgcaacgt tcacttcctt tcttctcgaa cggcggtggc
     2521 ggttggagtg agagaaaggt ttttcaattg agaaggcact tgtgtcttcc taaaaaggtc
     2581 ttttcctgtt tctatgtaaa acaaaaagag ctgcaatcct catttaggac tgcagctctt
     2641 cttttatatg cccatcgatc gcgtaaaatg atcggggttt ctaatttgca tcgattcaat
     2701 gactaattga ttccgtttat tgaaatgacc tgtgacggca atattgtatt ttccatcatc
     2761 cataattaac aatttatcga taatttcaat atctgcaact acacaattaa tcgattcatg
     2821 aattgtttgt aaagtaaagc gaaccaataa tggttgtgtt tttattactc ggattttatc
     2881 aagcaaacca acatatttgt tcatcacgat cacctcgcta aaattatagc aaaaacagct
     2941 aaaattgaga atttttattc gattttagat atgctataat gatcaagaca gtaaagtttg
     3001 tgacggtggc tatccgattg aaagtgaggt ggtgcgtatg tgcgtacttc caaaatctta
     3061 cagaaaggag aagccttttg cctgttgaaa cggcgctagg attaatgatt agtttcggtt
     3121 ctttgatcgc aacaatcatt tttggaatct tagcagttgt aaacaacaat caaaaaaaat
     3181 aaccgtcagc tttagccggt cgacgattat ttttcaaaaa taatattaag ctggtcaccg
     3241 tcttaaacgg tgctgttagg ggagttgtta gcgcaactcc tcttttcgta tccattataa
     3301 caactttatt gatcttttac aatcagaatg gtaaatcatc atcactaata tcgattgatt
     3361 gtccgccaaa tggattttgt gaagcgttca atccattgtt gttaggcgta gctacatccc
     3421 gttgttcaaa ggtggtaggg cttggactgc tggacgattg atcagcgtgc gcacggcttt
     3481 ctgtcgctgc tttactttct aatagttgga aattatcggc cacaacttct gtcacataca
     3541 cacgttgtcc ttgttggtta tcgtaggttc gggtttgaat ccgtccaaca acgccgatca
     3601 aactgccctt tttagctaat gtcgccatcg tttctgctgg tttgcgccaa atcacacagt
     3661 taataaaatc agcttctcga ttgccgtttt gattcgtgaa atttcggttg acagctaggg
     3721 taaaagtcgc tactgcagta ccactcgcgg tataacgtaa atctggatct ttggttaagc
     3781 gtcctactaa agttacatta ttgatcatct ttatctctcc atttcttgat tcatttgttg
     3841 tgatctttta aactgccgtt ttctttcttg attttcaatt ttgatcacac ttttcattag
     3901 gtgatcaatt tcgttgactg tatctgtaaa tgaaatggaa ttcgcaaacg tgttaactga
     3961 gcgatatttt tcccactgct tttccaaata gtcatttcct ctgatcttat ccatttctct
     4021 ttcataataa aaggacaacg gttttgtatc cccacgtttt gtaaatgtat ttcgaacact
     4081 tttgtataac tctttcgtat cgatattttc tagtttcgga atggtaaata agtcgtagta
     4141 atcttttgct ctcgaattat ctgtgccaaa gctgaacgta gcacttagct tttctgcaat
     4201 aatttgttct gttggataag ctggaatctc cactttttta tcttcaaaca ttaattggtg
     4261 atagtgtgta tctacttcag gataaataga atctcctgtt gtcacgtcca ttttaaacgg
     4321 tacacgtact ttctctaaat tagccaccaa tttaatttga aatccaggat aaaaatcggc
     4381 ttctcttgtt tccttcattc cttgaataga gaaaacaatg ccttcttttg tgggttttct
     4441 agttaaatca ttaaaaatgc tcttcaattt ttcttcagtt aatttgctat ttctaaatgt
     4501 cgtatcgata tcaattgttg tccttctatc gattccatat tttgatccta ataaaaagcc
     4561 gcctttcaaa atgaagtcat ttttgtactt tgattcagaa atttgttcaa ttagacgatc
     4621 catcataaaa tgccgttgca ctaactgtgc atttaatcct gtactctttg ctaaatttct
     4681 tgtttttgct gcaaattgtg tcggtgtcat ctttttcctc catcaatata agctttgcat
     4741 gtggtatttc atttcttttt ctacatgtag ttttgtcatg taatctctta atttacttgg
     4801 atctctcaat gggtcttcca tgtactcttt taatgcttct aatttcattt catattcaaa
     4861 ttcatatctc ggattccaaa tatcacaaag tgtacgctct ttatcataca tagtaatagg
     4921 atttccttga aaggaagttc cctctacttt tcctagttca taaacttctt tcttcacgaa
     4981 atgaaattgt aactgctgct cttttgtaat tgaacgatca acgttatagc catggggaaa
     5041 agttaagtga atgaatttcg gcatattcgt agaaagctgg taaatatcta aagtcgtttc
     5101 atgagagaag attcccctgg aaaatcgttg ctgaagccaa aataaatcat ctattccatg
     5161 accatcctct acataataac cacgatcaac tttgatcaat tttccttgat tcaccaatct
     5221 ttgtacagtg gttgctgcga ttccaaattt cctggcttcc ttcatagata aatttccatc
     5281 gcgaatagcc aacaattctt tcagtttatc ttgcatttac ttcccctccc ttcgttacaa
     5341 atacactaca tcaaatatat catagtagtt tatttgtaac aagattaacg tctatcgatc
     5401 taattcgatt tctttttgaa tgcgctgctg gttctttaat tgttgcagtc ttcttttatt
     5461 tgcacgtaat ttctcttgtt ctaaacgaat ttttgctttt tgattttcga tttcttgttg
     5521 tttgtgtgtt gtatttcgca taggataacc ccttcattat tttgtagtgc ggcgtgttct
     5581 tttcttatag ttcgtttttt tcttttcaga aaattcaaag cctgttgtaa agtcttcttt
     5641 taaaacgatc gttgcttcaa acgaaccttt ctgacctttt agtcctctga ctgattggct
     5701 tgttcggccg ttttgaatga tctctttcag ttgtgtggct gtgagtttct ttttggcaat
     5761 cgttgtccat aatttaaaat cacaggcttc agacgtacat gccgccacat tttgataaag
     5821 caagacttct tgttcccgac attttggaca aacacctaaa tgacgcgttg cttcctgttt
     5881 ttcactggct gctttcacct cttgtaacgt tgatccaaaa tctgttgact ggatttgtcc
     5941 cggtacgact tccaacaagt gagagacaaa tttttggata ttagctaaga aagtagcttg
     6001 tgttcctttc tgctcaccaa tctttaataa gtaactttcc catttggcgg tcatttcggc
     6061 acttgttagc agcggctctt tttccacggc ttggcaaagt aaaatccctt tatttgtcac
     6121 cacaagctta tttttttcta ccttgatata ttccttttgt ttcaaagttt caataatatt
     6181 tgctcgtgtt gcttctgtac cgattccttc tacttctttc aatatggatt gtgcttcttc
     6241 gctatccacg gtttttccag ctgttttcat cgctgtgatc aacgtacctt cggtataagg
     6301 ttgtggtggc ttcgtttctt tttcaaggac ttgtagatct accgtcaccg cttctccttt
     6361 tgtcacttta ggaagtagcg gttcttcttc cttttctttt ctgtcgctct ctttgccaaa
     6421 cagctttttc caacctagat ctactggtac ttttccgact gtctttagct gaagctgctg
     6481 aacttgcgtc aagatcgttg tttcttcata ggtatacttt tccgcaaaca tggccaccgt
     6541 tgtttttaac acttgttcat aaatagcctg ctgcagtgga cttaattgtt caaaagccgc
     6601 tgccgtcatg accgtctttg taggaatgat cgcgtaatgt tcctggactt tcttattatt
     6661 cacatatcga ctattcggtg tatgaatagg agtaggaatg gcttcggcct ttaaaaagtg
     6721 tttgtattcg tctaagtgat ccaacagata cgcatactcc ccttctgtaa tataaggggt
     6781 atctgttcgt gggtagctta gatatttccc ttcatacaac ccttgcatgg cttccaaagt
     6841 atctttggca ctagctttca tgagctgatt cattttagat tgaaggctgg atagagaaaa
     6901 aagacgtgga cttgccgcac tttttttctc ggtttcaact tggtggatcg tacctggctg
     6961 ttttcccaat tgaaccccct tttcttttaa ataatcttca aatgcttctt gcgtcgcttg
     7021 ggtttcattc ggatcaagct tcgcatcaaa tgcgccattt tgagcgatta cttgggcttt
     7081 tccttcaaaa tatggttctt tttggaagtt tctaatcttt tcctgtaatt gataaatcat
     7141 gtaaagcgtt ggggtttgga ctctgcctaa ggaaaaactt cctggtatgc ctttttgctg
     7201 caagagtaaa ctatacagcg ggctaccgtt catgccgatc aaccaatcgg cgatctggcg
     7261 tgcttgtgct tcttcaaatt ttgggtaggt ctcttctgca tctcgtaact gctggaatcc
     7321 ctgataaatg gcttcttttt ctaaggagtt gatccaaagt cttttaaata tctttccatg
     7381 atcggcgcct gcttgttgaa tgatcgtcca agctatcagt tccccttcac gatcgctatc
     7441 tgtcgcaata ataatggtgt cggccgattg aagttgctgt ttcaccacct tgaattgctt
     7501 tttcttatct ttaggaacat gatagtgata atgtgtaggg aaaataggca aatgctctaa
     7561 tgaccattgt ttccaatttt catcgtacat atcgggcgaa tctaactcga ctaaatgacc
     7621 aaaaccatac gtgatcacag tttctcctga aaacaatcga tcttggatct caaaataccc
     7681 atcttttcgg gtagctttag aaaagctatc ggcataagcc ttcgcttgtg acggtttttc
     7741 tgctaaaata acggttttca tcatgacgat ttcactccct ttcttgctgt ctaccttgtt
     7801 gaaacaacat ggcacgtttt tgctttctga tctcttgttg gtagatttca tgcttcaatt
     7861 gatcattcca atcctttttt acttcaggta caaattcatg ttcatgattt ttatagcctt
     7921 tgtagtattg ttctgcttta taagagaaaa gaatctgagc attaatatcc cctttttctt
     7981 gataaaaacg atcaaaatcg tacgtaccat ccacgcgttc acaagctttt aattcttttg
     8041 cgacttctct acaaatatct ttcaaattaa tagcttctat tgaatcgtta acagaaagct
     8101 gctgaccaaa agacgcattt actttgtgag cggaaaccaa ttggttcgtt aattgcagat
     8161 tgttttctgt cttgtgtatg gccataatag cttctggtgt gatcttttct tcttttgcga
     8221 cttcttcata aatgactttg tattcttttg gcacttgcca atcattagcg attcctcgtt
     8281 cacaacgtac tttctgattc gtaaatggat caactgcgcc cattaattgt tctttttcgt
     8341 agaaaatatg tcctgcacgg tcattatcca cacaaatctc gatggattga ggaaaggctt
     8401 gtttctttct caattcagat actgcctcac caaaataatg actgatcaca tggtgtttta
     8461 acccttccat cgaaactaac cacgtatcgt ttaactgatc acgatttagt gcagcatagc
     8521 taagtaaatc aatactactc tcaaaaaatt ttaactgctt agggtcaccg atctttaggt
     8581 taaagccatg attcgctgtc gaatttttat ctatatgctt atatgtgcct ctctcaccaa
     8641 aacgttcata atcaatgaac gttccttgat aactcgcccc tactgtctca tttgtctgtg
     8701 gatctttcca taaaaaggcg accgcatgat ctaaacgatc gtctcgaact aagaaatttt
     8761 gccgttcatg ttttggcttt agttcggcaa tcaacccttc tttttctgca taatcaatca
     8821 gccactctgg tagttttcgt tcttccttca gataattcct tcctttttct gtgagtggtt
     8881 gataatcaac ggggtgatct ttgtactttt ctgtctcaaa atgaagattt tccactcgtt
     8941 ctgttttgtc ttcattcttt agaataaaat cagtggctgc tttaaattgt tgcttagggt
     9001 cttggatctc tttgtctatg aaaaaaagtt tagctaattc aataatgtcg ccactaatat
     9061 tttgactgtt ccaataaaaa cgttgttttc ttcgatcaaa aacaaatgaa tcatggtctt
     9121 ctaagcgata gtctcgtcct ttattgacta cagccattcc attgtttcga gcaaaatcca
     9181 ctatatcgat ctgtttacat cgatcaataa gactcatttt tttctcggtc attttaaaac
     9241 ctcccaaaag aaaaaagact atccgttttt cagatagtct tttttacttg ttttatacac
     9301 gttctttttg tgacaattgt tgttcataat agatttcatt ttttcgtttt gccacttctg
     9361 acactgtctc aagtgaaaat cggaatgaat ttcctagcgc aaattcttct gaaggtaacg
     9421 ccgtttggat ctcttttcca taactttttc cttttaaaat tacttgatta tgctgtggat
     9481 cttcaataaa cgccgcatcc ttcaaatgta acgttcgacc cgtatcttct tccattactt
     9541 tcccgtcttt cttgtcgatc caaagtgaac caacaggaac aatattcttt tgttctgccg
     9601 tttctacagt atttttatgc tcctgcttca cttctgctgg ttggatctct ggttctcttt
     9661 ctctgtattg attgatttca ttagaaatgg tttgaatgaa ttcatttgcg acacggctga
     9721 catcattcat gattttccca cgttccgcag cttccttatc atccaacgtc tgaccattct
     9781 tcgtccatgc tgcaatataa ggaatagctt tctccgaagt gtccattcca aaatgtttgc
     9841 acacaatata agaagtcatt tctgcctgca attctttagt tgcggtatct aattctgaaa
     9901 atttgtgcat tcttgcatgg gctaattcat gaatacttgt agctaaatgc tgcgtaggag
     9961 tatttcgagt attcatgacg atttcttctt cgccagtaaa ttcgttacga acatatgccc
    10021 cccgtgcttg tcctaattct ctttgataga cttcactttc tgtcatatct cgaatttgta
    10081 ttccaatatt gttggctacc gcttgaatcc cagcttgaag ttctgactgt ccttttttat
    10141 ccaattgaaa atcaaagaca cggttcggaa agattttcgg atagtcttca ggttttaact
    10201 gtgtttgtgt gatatcaaac actttttcaa tcgtgtagta aggttttttg atcacttcga
    10261 tttgaccact ttcgattcgt tttttatctt ctggtgtagc ttctgtttct cgaacaaagc
    10321 cacgtccttt ctcataaaat cctttgaccg tttttctgac aaagatcttg ttcccttgtt
    10381 cccctttttt gatatagatt ccttcttttt tcaacgcagc acgacctaaa caagccgttg
    10441 cacctggaaa ttgttcctca atcaataatt gatttctaaa ggatcgttct ggaaaacgag
    10501 acataaaatt caaaagttct ttcacgtctt caggagaatg ggtatgcttt ttcaccgcat
    10561 ccagtgcctg ttgcgaaagg gatttgactt cttgcttaat ctcttctgcc gttcgttttt
    10621 gatagccctt ttgttttgtt tttgtttctt tcgactgcat ttgttctttc gaaacaggtt
    10681 catttttgac cgtttgtttt tgttcctttt caggtacttg ttcgcacaat tttgctaact
    10741 ctaaatagat gggttgatta ctgttgacgc catcccctag atccattcgg atctgatcga
    10801 ctacttctcc gttcttcact tcttcaaaat agaatttata ataacctgct tctgcggcgt
    10861 attggttatc caacgttttg agttgatcga tcaattctaa tgttacgatc tccccaccat
    10921 aacttttcaa tcctttttct ggatcatttt cgttgaattc tactcgatag aaatgatcgt
    10981 tttctttctc cttcaaagaa tcagttggcg cagcctcttc aagtaattca cgaacagctg
    11041 ctttttcctc ttccgacgtg gcttggtatt tttttgattc ctcaaatccc ccgttttcat
    11101 tcagttctaa agcaactttt cgatagtctt tcaaaaaata aatggttgga ttttttttcc
    11161 attgcgtcac tcgtccgctg tcaataattg cttgtacttc aggaagaaga ttttcttttc
    11221 ctttttctga tctttctttc agctgttcta atgtttccaa tttccgttgg tattttcgga
    11281 tcgtagcggc tgaaaaagtc gattcacctt tttcaaagcg ttcgatttct tctttgattc
    11341 ttggaatatt ttcgattgtc atatccaggc cgccgtactt gttatagccc atttccttta
    11401 gctcttcttg tctttcaagt ttttctacct gtttttcttg ttgcttgatc tcttctctca
    11461 gactatgcac tttgttttct aaacgttctt gttgcctaaa ccaagaatgc ccatttctct
    11521 tatcgttcat tggctgaccg ttggtctttc tctggtgttc aaacatcttc tcaatttgtt
    11581 cgtttaattc attttgcaat cgtactaatt ttcttttgga ttttcccaag cgactattgt
    11641 ctagtggttc tttttcttct gccatgtcc
//
LOCUS       DAZKBH010000083        11667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000083 DAZKBH010000000
VERSION     DAZKBH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.083"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG8OO_002097"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG8OO_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9045958.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACG8OO_002098"
     CDS             complement(322..891)
                     /locus_tag="ACG8OO_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFP9045959.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACG8OO_002099"
     CDS             1077..1979
                     /locus_tag="ACG8OO_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9045960.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG8OO_002100"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG8OO_002100"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9045961.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG8OO_002101"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG8OO_002101"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9045962.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG8OO_002102"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG8OO_002102"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9045963.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACG8OO_002103"
     CDS             8547..8732
                     /locus_tag="ACG8OO_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFP9045964.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACG8OO_002104"
     CDS             complement(8794..9990)
                     /locus_tag="ACG8OO_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9045965.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACG8OO_002105"
     CDS             complement(10160..11596)
                     /locus_tag="ACG8OO_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9045966.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3449 a         2486 c         2047 g         3685 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaa
//
LOCUS       DAZKBH010000084        11427 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000084 DAZKBH010000000
VERSION     DAZKBH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.084"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..574)
                     /locus_tag="ACG8OO_002106"
     CDS             complement(29..574)
                     /locus_tag="ACG8OO_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP9045967.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(586..1278)
                     /locus_tag="ACG8OO_002107"
     CDS             complement(586..1278)
                     /locus_tag="ACG8OO_002107"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9045968.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACG8OO_002108"
     CDS             complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACG8OO_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFP9045969.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1594..2157)
                     /locus_tag="ACG8OO_002109"
     CDS             complement(1594..2157)
                     /locus_tag="ACG8OO_002109"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9045970.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2385..3047
                     /locus_tag="ACG8OO_002110"
     CDS             2385..3047
                     /locus_tag="ACG8OO_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFP9045971.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3171..4367
                     /locus_tag="ACG8OO_002111"
     CDS             3171..4367
                     /locus_tag="ACG8OO_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFP9045972.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4468..5844)
                     /locus_tag="ACG8OO_002112"
     CDS             complement(4468..5844)
                     /locus_tag="ACG8OO_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9045973.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5881..6747)
                     /locus_tag="ACG8OO_002113"
     CDS             complement(5881..6747)
                     /locus_tag="ACG8OO_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9045974.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6836..7861)
                     /locus_tag="ACG8OO_002114"
     CDS             complement(6836..7861)
                     /locus_tag="ACG8OO_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9045975.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7879..8553)
                     /locus_tag="ACG8OO_002115"
     CDS             complement(7879..8553)
                     /locus_tag="ACG8OO_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9045976.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8737..9630)
                     /locus_tag="ACG8OO_002116"
     CDS             complement(8737..9630)
                     /locus_tag="ACG8OO_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9045977.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9704..11401)
                     /locus_tag="ACG8OO_002117"
     CDS             complement(9704..11401)
                     /locus_tag="ACG8OO_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9045978.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3385 a         2514 c         1963 g         3565 t
ORIGIN      
        1 ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa
       61 aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggca tctttgaaaa
      121 tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg
      181 tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat
      241 ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct
      301 catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca
      361 cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat
      421 caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct
      481 ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat
      541 catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt
      601 gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa
      661 gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat
      721 attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc
      781 agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt
      841 aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact
      901 gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc ctggtttgta
      961 gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc
     1021 tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc
     1081 gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg
     1141 tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc
     1201 ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc
     1261 gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa
     1321 aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag agttccattt
     1381 ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc
     1441 tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg
     1501 ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt
     1561 aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca atagttccca
     1621 gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg
     1681 cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg
     1741 attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc
     1801 tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc
     1861 ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc
     1921 agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag
     1981 acgatcttgt tcgtcaaaag caatgatccc gacaccacca ggatgaaata ccagttctct
     2041 cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc
     2101 gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc
     2161 ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa
     2221 actccttatt agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc
     2281 tttagtccga tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg
     2341 aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt
     2401 ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt
     2461 ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc
     2521 gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact
     2581 atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa
     2641 caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa
     2701 tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag
     2761 aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg
     2821 atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg
     2881 aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt
     2941 atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac
     3001 agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat
     3061 aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta
     3121 gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata
     3181 aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca
     3241 acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc
     3301 tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac
     3361 aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg
     3421 gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag
     3481 cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag
     3541 ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg
     3601 tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca
     3661 agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag
     3721 aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg
     3781 gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag
     3841 aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc
     3901 gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac
     3961 cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta
     4021 tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc
     4081 aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga
     4141 aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa
     4201 tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga
     4261 cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac
     4321 acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct
     4381 tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta
     4441 tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc
     4501 atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac
     4561 agtttgcaga cgaagcatca atacaataca agtcaagatg gagacgattg gcaatagcgg
     4621 aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag
     4681 taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa
     4741 tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc
     4801 ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc
     4861 cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag
     4921 tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct
     4981 cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa
     5041 gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct
     5101 tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc
     5161 caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat
     5221 gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat
     5281 gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag
     5341 cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc
     5401 attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa
     5461 aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc
     5521 cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata
     5581 ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga
     5641 caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg
     5701 attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag
     5761 catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat
     5821 ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga
     5881 ttaaactgtt ttcatatacc ctaaaaggat accacctaaa atgactaaaa ggcaaccaac
     5941 aataacataa atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa
     6001 agtagagata atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt
     6061 gactgttaga agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt
     6121 ccacacaaac tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat
     6181 tccgatactt tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat
     6241 ggtatataca ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc
     6301 aagcatttta ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc
     6361 accgcttaca agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca
     6421 ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa
     6481 tggtagccct acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac
     6541 actccaagct aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact
     6601 aacaacgaaa aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg
     6661 ttgattggca tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat
     6721 agggactaat gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa
     6781 cttatcagcg aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac
     6841 gtttttcatt ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt
     6901 cagttgattc agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa
     6961 gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat
     7021 caaagatccg tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc
     7081 tgactccttg cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt
     7141 tgatggcatt actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa
     7201 cagtatgatc ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct
     7261 gactgcgtat cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc
     7321 gcgagaaact gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg
     7381 ataattcatt ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa
     7441 ctagcaattt agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat
     7501 ctttttggtc ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca
     7561 gctgattgat atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat
     7621 tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca
     7681 agtaaccacc cagcagataa aaaatcagaa agacgccctc aatcaaagct aaataagcaa
     7741 ttgtgctcaa atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa
     7801 taaaaaacag ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca
     7861 tcttaaccgc tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata
     7921 tccttggata cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt
     7981 attgtcatct acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa
     8041 gatctttcca ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc
     8101 aatgatctct ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg
     8161 gctcatcatt tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat
     8221 ttttgcaatc agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt
     8281 catagccatg atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt
     8341 cgatacttca cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc
     8401 tagtaataca agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt
     8461 cgttccaaag gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat
     8521 ctcttcgtct tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc
     8581 tactacaata tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt
     8641 agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag
     8701 cttaaattta ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg
     8761 tttttgtctt ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat
     8821 acctagaagg agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga
     8881 agagacttct tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac
     8941 ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt
     9001 atttgttttt gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc
     9061 atacacttca agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt
     9121 tttctcagca ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg
     9181 aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc
     9241 aaataaaggc aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc
     9301 gtaatattct tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac
     9361 ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac
     9421 tgctaattgt tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc
     9481 gatccttctt tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac
     9541 tgcagctccg gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt
     9601 ttgaggtcta ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc
     9661 agcaaccttt ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca
     9721 ttttagattt acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga
     9781 atgccaagag cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc
     9841 tttctggccc tggattttct actcctacga tctgaatttc ttcattatag cttccataag
     9901 agatgataaa ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca
     9961 tggtctcttt catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc
    10021 catctgcgac aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg
    10081 attcataaac agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt
    10141 cattccacaa ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt
    10201 caaactttct tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa
    10261 attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta
    10321 gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta
    10381 atctacctaa agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa
    10441 acccgttgct tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg
    10501 ctcgcacttc tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt
    10561 tgtaattttt gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag
    10621 gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt
    10681 cttgttctcc agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca
    10741 tctgtttatc tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg
    10801 tgtctttcca tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca
    10861 tcataacaac cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga
    10921 aaagatagac tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata
    10981 ccgcttctcc agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa
    11041 accaactttc tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt
    11101 cttctcgttc cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt
    11161 caaacaaatc aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc
    11221 cgctattcgg tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca
    11281 cttcacttgg tttttgtgcg acttcttcgg ctttggctag ttttccgttc ccgaaaatcc
    11341 ctattcccgc agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca
    11401 tttttttctc cccgttcttt ttttatt
//
LOCUS       DAZKBH010000085        11210 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000085 DAZKBH010000000
VERSION     DAZKBH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.085"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG8OO_002118"
     CDS             <1..395
                     /locus_tag="ACG8OO_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFP9045979.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACG8OO_002119"
     CDS             398..1228
                     /locus_tag="ACG8OO_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFP9045980.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACG8OO_002120"
     CDS             1244..2095
                     /locus_tag="ACG8OO_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9045981.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACG8OO_002121"
     CDS             2092..2451
                     /locus_tag="ACG8OO_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFP9045982.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACG8OO_002122"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACG8OO_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACG8OO_002123"
     CDS             2624..2929
                     /locus_tag="ACG8OO_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045983.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACG8OO_002124"
     CDS             2929..3240
                     /locus_tag="ACG8OO_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFP9045984.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACG8OO_002125"
     CDS             3237..3410
                     /locus_tag="ACG8OO_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045985.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACG8OO_002126"
     CDS             3407..3823
                     /locus_tag="ACG8OO_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFP9045986.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACG8OO_002127"
     CDS             3823..4491
                     /locus_tag="ACG8OO_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFP9045987.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACG8OO_002128"
     CDS             4488..4865
                     /locus_tag="ACG8OO_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045988.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACG8OO_002129"
     CDS             4862..5161
                     /locus_tag="ACG8OO_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045989.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACG8OO_002130"
     CDS             5151..5309
                     /locus_tag="ACG8OO_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045990.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACG8OO_002131"
     CDS             5403..5819
                     /locus_tag="ACG8OO_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9045991.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            6379..6567
                     /locus_tag="ACG8OO_002132"
     CDS             6379..6567
                     /locus_tag="ACG8OO_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045992.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            6557..6817
                     /locus_tag="ACG8OO_002133"
     CDS             6557..6817
                     /locus_tag="ACG8OO_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFP9045993.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            6858..7085
                     /locus_tag="ACG8OO_002134"
     CDS             6858..7085
                     /locus_tag="ACG8OO_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045994.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            7150..7722
                     /locus_tag="ACG8OO_002135"
     CDS             7150..7722
                     /locus_tag="ACG8OO_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045995.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            7723..8034
                     /locus_tag="ACG8OO_002136"
     CDS             7723..8034
                     /locus_tag="ACG8OO_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9045996.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            8025..8285
                     /locus_tag="ACG8OO_002137"
     CDS             8025..8285
                     /locus_tag="ACG8OO_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045997.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            8446..8805
                     /locus_tag="ACG8OO_002138"
     CDS             8446..8805
                     /locus_tag="ACG8OO_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9045998.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            8802..10445
                     /locus_tag="ACG8OO_002139"
     CDS             8802..10445
                     /locus_tag="ACG8OO_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9045999.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            10480..>11210
                     /locus_tag="ACG8OO_002140"
     CDS             10480..>11210
                     /locus_tag="ACG8OO_002140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046000.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYN"
BASE COUNT         4091 a         1619 c         2481 g         3019 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 gaattttaac tccttctaaa ttgttcttat tatctatcat cagttgctgt ctattgtcat
     6061 tatgtcactg tggtggaaag gtgtatcgct catctaaaat ttaggtgcaa actgcaaggt
     6121 tcgattcctt gccagtgact ttggtaaccg aggcatcggc ggtttaaaaa tataggggtg
     6181 cgcaatttcg tacgcgtttt gtgcatcttg taggttgcta ttacataatt ggttgggtta
     6241 gattgagttt tgggattcgg tacaaatgaa tcgtcaaatg actcaagcac aggatcggaa
     6301 acgtccctgc ctgtgcatta catattagat cactctttga gtggtctttt tattttaaaa
     6361 aagagaggat tttgaaaaat ggaattgatt gtatttacta ataacggaca aacatatcat
     6421 ttttatgaag tggagaattt taagccaaca acaacaggat ttagttttat ttatactggg
     6481 aaggctacgg gagttactcg ttcagctacg tttaacaata catcaacagc tggttatgcg
     6541 cttgcggagg tacaaaatga atcatgagaa gtttatcgaa aaatgcaagg ctattgtacg
     6601 tgaaagaatt gaaaatgaga ttgctgacct aagtggagca gtgcctgagt ttagtatttt
     6661 catagtctgg tcatgcaaaa cactgcaaaa tagcaaagca ttagtcagcg ctagcttaaa
     6721 aggagcaccg tattttgaaa ttacgttgaa cggggacaaa ggtgaaatct atgtagatac
     6781 ttatctcaaa aaatcaaatg aatgtatcaa agtctagcag gtgctaggct ttttctttac
     6841 ataaaggagg ctgcataatg agaaactact ggtatgtatc actaactaat gaatatcctc
     6901 gaaccattgg tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact
     6961 ctattgtcga aatgctaaga gaagctacac caaacgaagt ggataaatgc aagctgatat
     7021 attgcggtca tggctattgg aaagacgagt atatccaata caacattgag aggtacatca
     7081 aatgaatgat aactatgatt acatcaagtt gttagcaagt aattattaga tgtttttagg
     7141 aggaaaacta tgaaattaaa agattatatc agagaagggt ataacgttgt aactgcaccc
     7201 aatctagctt acaaaattca agaagattat cccaatgctt tagtgtttac tgatagagct
     7261 ttagatgcca ttcctatagg gaagttatta gtagatcgtt tttatagcaa taatccagct
     7321 gttcttagtg ctaagccact tcaaaacgct tatgctattg aacgctttac ttgcgagttt
     7381 actggatatg agacggtagt accaatgaca ggtgagacag gaagaaaagt aattcaacag
     7441 atcaaaaaga ggattgctga attgacgtta gatgatggaa gtaatacgaa ccttctagaa
     7501 atcaaactaa gagatactga ctcagtacca gaagtttatt ataaaggtca acgaatggat
     7561 gagatgccga taggtcttgt cgatattact tatcattggg atacggaaga atatgaatgc
     7621 caagtaccaa aaggagctaa cgacatcact attgaatatt atgattcaca taataacaag
     7681 tacttagata gaaaaatcat tgggcacaag agagatatgt aaatgattga tgtttcaagt
     7741 aaacaagcaa gagctaggtt ctatggatca actgagtgga ggaaacttag aagtcaatgc
     7801 ttagagcgag atcattatga gtgccaatgg tgtaaagcag agggtagact aacaactcaa
     7861 catgattcaa ttcttgaagt ggatcatata aaggagctgg agtatcatcc ggatttagct
     7921 ctagatataa acaacctaag aaccttatgc aaagattgcc ataacaaaag gcacaaccgt
     7981 atgaaatatc gtggccaaca aaagaaaaga aagtgggatg atgaatggtg gtaagaaaaa
     8041 cagcaaacag agatgatgtc aaacgagcag aagcaatcgc tatggttgat aaggctattg
     8101 ataagttggt tctatttaag agtcagctca ttgaaggaac aacaactgta gaggatatat
     8161 ctatcaacaa tagactaata ggtaacgcta atgaagtaac agggacattt aatattaatg
     8221 tagacattga ttataccttg agtccatttg cacatattgt agaaagcaac gagcaagacg
     8281 gctgataaat cagtataaag cgaagatata tagagtgaaa tacccccggt caaaagattt
     8341 tgagaagaaa taaaattttt gggaaccggt ggatggggtc aactctgcaa ataaatgatg
     8401 ctattttcac acgaccccct ccccacgtag aaaggaagtg agttgatggc tgatctgcaa
     8461 gagagaaata ggcttgtttc tgctgaagaa aggcgtctaa ataaattgtt taaggatatt
     8521 gataaggata aaaagaaggt cgtttctggt ttgattacgc aagcggcacg cttaaaaatt
     8581 cttcttgatg aaatgtggat tgatatttcc gaaaaaggtg attatgagct tttctctcag
     8641 tcagaaaatc aaaaacctta cgagcgggaa cgaccagttg ctaaacagta taattcgaga
     8701 gaccaagcat atcaacgtgt aattaagcaa cttactgact atttgccaga agaaaagcgc
     8761 gaacctacaa aaaatgcagc gctagatggt agtgatttgc tgtgacaaca ttaaagccat
     8821 tctattttga tgaatacgta gatttatacg aaaaaggggt aatccctttt aataaagaaa
     8881 gaattcagtt aattacttat ttaaaaaacg aagtgctagt tcgggatgat atctattttg
     8941 atgaagaaat gattcaaaag tttatcaagt acactgaaaa aaattttttt cctttagcta
     9001 aatatcaaaa atttatcaca cctttcattt tctgctatca aaaagaggac gacgaggtgt
     9061 tttttgatga aattctaaat agtattgcgc gaggcggcgg gaaaaatggg ttcatgtcag
     9121 caagagactc attttttatt agccctctct acggtgtcag aaattatgat gtaacgatta
     9181 cagcgaattc tgagaagcag ggaaaggtat cttttaaaga agtttatgaa atggtacaaa
     9241 tgcgcaaact ggaaaaacaa ttttatttga ctaagatggc tatagtcaac aatgtaacta
     9301 attcgatttt tagttatagg acaaacaatc ctaagactat ggatagtgca agagatggct
     9361 gcttagaatt tgatgaaatc catcagtttg aatcttcaga cctagttgat attcagcgta
     9421 gtgggctagg gaaaataaaa catcctcgaa ctttttataa tggtacaaat ggtcatgtta
     9481 gagaaggttt ttatgacaaa atgttggaac gagcacaaaa gatttttagt ggagagaaca
     9541 agaatgatcg cttgtttccc tttatttgta aactggataa cattgatgaa atggataatc
     9601 ctaaaatgtg gtcaaaggct aatccaatgt tcgaagaaga ttcagcatat tcaaaacgtc
     9661 tgttttctac agtgatgaaa gaatataaaa aactagaaga tgagccatca ggtaggcgtg
     9721 aatttgttgt taaacgaatg aattttacag aaggtgatgc agaatcagat attacgactc
     9781 atgaaaaatt aatggctact aaacaagaat tgccaaattt gaatggtcgt tcttgtgtgg
     9841 ctggatttga ttatgcaagt attcgtgatt ttgcaacagt gggtttgctt tttaaaattg
     9901 atgataaatt tatctggcag caacatagtt ttgctaggaa aaagtttcta gatacattca
     9961 aactgaaagc gcctattaag gattgggaga aaaaaggttt agtgacaatc gttgatgagc
    10021 catcaattga tcctcgtcat ttaattgatt ggttaaatga aaaaagaaaa atccatcaaa
    10081 tagaaatcgt ttgtgccgac ggatttcgta tggatttgct gaaacctctt ctagagaaag
    10141 aatcatataa ttttgagttt atcagaaata taagaggtgt gcagtcaaaa gttgctccta
    10201 ttattgaaga tggttttgca aatgaacggt ttatttttgg tgatgatcct atgatgcgtt
    10261 ggtatacaaa taattcgttt atcaaagaag acaaattagg gaataaaaca tttttgaaaa
    10321 aagagccagt aaggcgtaaa acagatgggt ttcatgcttt tttggcagct ttatataaac
    10381 gagaagaaat aactgatatg gattatgagg aagcatttga tatgttggat gagattgaat
    10441 tttaaggtat actccttatt gaaaggggta gttatagaag tgaaaaagag atattttcta
    10501 tttgttacaa tatttataat aatagcaatt tgtgttttga cagtattttt accacaaatt
    10561 actgagtatt taaattatcg gatatttacc acaaaagtag atgatagcgt tactagttta
    10621 agtaggctag aatttataaa aatatgtgca tctgtgtttg gtcctatttt gactgtaatg
    10681 gtatttgtga atactttaaa tatgcagaaa aaaacagagc aaaagcaaaa tgagcttgaa
    10741 gtaaagcaag ctaaggctga gaacgagaga gcaaaaaaag agctgttaac acagatagat
    10801 cgtgagttct attttttatt aaatatgttt attgaaatac aaaaagatca aaatgccaaa
    10861 caagctgtta ttgctttaca gaagttggca atttttaacg aaaagggttt taatcaagac
    10921 tatgcattta cttctaaaga tttatcagat agtgaaattg ggagtacaat tgaacaatca
    10981 ggaccgctga agggcacagt tgaatatata aaatttgaaa catttgatgt aagcaagtcg
    11041 atggggaatt atcaaacaaa aacaaaagac gttgctacaa gtttacacga tcagaaagta
    11101 gctatattga atgaacaata tgaaaagatg caaatatatc ttggacggta cttcaaaatg
    11161 tttcaccgaa tagttaaaac attaaatgag tattatgacg attataatga
//
LOCUS       DAZKBH010000086        11184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000086 DAZKBH010000000
VERSION     DAZKBH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.086"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8OO_002141"
     CDS             6..305
                     /locus_tag="ACG8OO_002141"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9046001.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8OO_002142"
     CDS             378..1739
                     /locus_tag="ACG8OO_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9046002.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8OO_002143"
     CDS             1761..2021
                     /locus_tag="ACG8OO_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046003.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8OO_002144"
     CDS             2035..2787
                     /locus_tag="ACG8OO_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFP9046004.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8OO_002145"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8OO_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9046005.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8OO_002146"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8OO_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFP9046006.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8OO_002147"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8OO_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9046007.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8OO_002148"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8OO_002148"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9046008.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8OO_002149"
     CDS             7161..8558
                     /locus_tag="ACG8OO_002149"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9046009.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8OO_002150"
     CDS             8639..9079
                     /locus_tag="ACG8OO_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9046010.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8OO_002151"
     CDS             9227..9553
                     /locus_tag="ACG8OO_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9046011.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8OO_002152"
     CDS             9554..10303
                     /locus_tag="ACG8OO_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFP9046012.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8OO_002153"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8OO_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9046013.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3551 a         1957 c         2295 g         3381 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgtt
//
LOCUS       DAZKBH010000087        11110 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000087 DAZKBH010000000
VERSION     DAZKBH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.087"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1331
                     /gene="lysA"
                     /locus_tag="ACG8OO_002154"
     CDS             72..1331
                     /gene="lysA"
                     /locus_tag="ACG8OO_002154"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9046014.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1318..2118
                     /gene="dapF"
                     /locus_tag="ACG8OO_002155"
     CDS             1318..2118
                     /gene="dapF"
                     /locus_tag="ACG8OO_002155"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9046015.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2121..3002
                     /gene="dapA"
                     /locus_tag="ACG8OO_002156"
     CDS             2121..3002
                     /gene="dapA"
                     /locus_tag="ACG8OO_002156"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9046016.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2999..3715
                     /gene="dapB"
                     /locus_tag="ACG8OO_002157"
     CDS             2999..3715
                     /gene="dapB"
                     /locus_tag="ACG8OO_002157"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9046017.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3721..4416
                     /gene="dapD"
                     /locus_tag="ACG8OO_002158"
     CDS             3721..4416
                     /gene="dapD"
                     /locus_tag="ACG8OO_002158"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFP9046018.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4432..5433
                     /locus_tag="ACG8OO_002159"
     CDS             4432..5433
                     /locus_tag="ACG8OO_002159"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9046019.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5430..6791
                     /locus_tag="ACG8OO_002160"
     CDS             5430..6791
                     /locus_tag="ACG8OO_002160"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFP9046020.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6751..7902
                     /locus_tag="ACG8OO_002161"
     CDS             6751..7902
                     /locus_tag="ACG8OO_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9046021.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7877..9046
                     /locus_tag="ACG8OO_002162"
     CDS             7877..9046
                     /locus_tag="ACG8OO_002162"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9046022.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9221..10600
                     /locus_tag="ACG8OO_002163"
     CDS             9221..10600
                     /locus_tag="ACG8OO_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9046023.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10881..11110
                     /locus_tag="ACG8OO_002164"
                     /pseudo
     CDS             10881..11110
                     /locus_tag="ACG8OO_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3697 a         1944 c         2494 g         2975 t
ORIGIN      
        1 aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga
       61 ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa
      121 agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat
      181 tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat
      241 tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca
      301 gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc
      361 atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata
      421 ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa
      481 aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata
      541 tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac
      601 aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata
      661 tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt
      721 acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt
      781 atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata
      841 cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta
      901 gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac
      961 cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt
     1021 atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag
     1081 tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc
     1141 cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga
     1201 tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat
     1261 caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg
     1321 atccttcata aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac
     1381 acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tacagacgga
     1441 ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc
     1501 gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg
     1561 atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg
     1621 gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa
     1681 atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa
     1741 gtaacgatca ctagtttatt tttaggaacg attcatacag ttgtttttgt tgaaaatgcg
     1801 cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa
     1861 aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat
     1921 gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt
     1981 gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac
     2041 ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca
     2101 agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga
     2161 atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg
     2221 aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat
     2281 ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga
     2341 ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt
     2401 ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag
     2461 gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata
     2521 atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac
     2581 atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg
     2641 ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag
     2701 taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag
     2761 tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat
     2821 taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag
     2881 cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt
     2941 caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat
     3001 gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga
     3061 aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt
     3121 tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct
     3181 ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc
     3241 acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt
     3301 ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg
     3361 ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac
     3421 tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg
     3481 gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt
     3541 acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga
     3601 atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga
     3661 tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac
     3721 gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa
     3781 gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga
     3841 agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca
     3901 gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta
     3961 gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca
     4021 atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt
     4081 gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac
     4141 tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg
     4201 gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat
     4261 atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa
     4321 actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa
     4381 aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa
     4441 atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta
     4501 gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt
     4561 aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt
     4621 gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca
     4681 atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg
     4741 gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt
     4801 gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc
     4861 tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa
     4921 ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat
     4981 attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa
     5041 gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca
     5101 gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg
     5161 caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa
     5221 ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat
     5281 atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat
     5341 acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct
     5401 ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg
     5461 gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag
     5521 atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca
     5581 aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac
     5641 ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt
     5701 tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg
     5761 aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg
     5821 gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc
     5881 aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc
     5941 cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg
     6001 acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg
     6061 atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg
     6121 aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag
     6181 cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc
     6241 ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag
     6301 gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg
     6361 aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac
     6421 atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata
     6481 agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga
     6541 tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat
     6601 ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag
     6661 gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga
     6721 agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat
     6781 caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta
     6841 aaaaacagcg gggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat
     6901 aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa
     6961 gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac
     7021 ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt
     7081 atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc
     7141 gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta
     7201 tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc
     7261 agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga
     7321 atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa
     7381 ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccgc
     7441 aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg
     7501 caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact
     7561 atttcttttc gctataatga cggctatcca gctgtaatca accatagagg cttatatgca
     7621 gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat
     7681 tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc
     7741 cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa
     7801 tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa
     7861 gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat
     7921 agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa
     7981 actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc
     8041 agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga
     8101 agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt
     8161 tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga
     8221 aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact
     8281 agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga
     8341 acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta
     8401 tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt
     8461 tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag
     8521 tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt
     8581 atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct
     8641 tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca
     8701 aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca
     8761 atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac
     8821 ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc
     8881 ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc
     8941 atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt
     9001 acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat
     9061 caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt
     9121 tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt
     9181 ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt
     9241 cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg
     9301 tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg
     9361 tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc
     9421 agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga
     9481 tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc
     9541 ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc
     9601 agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa
     9661 attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga
     9721 agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag
     9781 acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga
     9841 aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt
     9901 tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg
     9961 tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat
    10021 ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac
    10081 acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg
    10141 tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg
    10201 cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt
    10261 gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac
    10321 gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt
    10381 ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac
    10441 aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc
    10501 agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc
    10561 cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa
    10621 gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc
    10681 ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat
    10741 tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa
    10801 tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag
    10861 atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga
    10921 ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga
    10981 ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca
    11041 agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc
    11101 gaggaaaagt
//
LOCUS       DAZKBH010000088        10726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000088 DAZKBH010000000
VERSION     DAZKBH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.089"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..1091)
                     /locus_tag="ACG8OO_002165"
     CDS             complement(243..1091)
                     /locus_tag="ACG8OO_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9046024.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1255..2157
                     /gene="gnd"
                     /locus_tag="ACG8OO_002166"
     CDS             1255..2157
                     /gene="gnd"
                     /locus_tag="ACG8OO_002166"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFP9046025.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2159..3160
                     /locus_tag="ACG8OO_002167"
     CDS             2159..3160
                     /locus_tag="ACG8OO_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFP9046026.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3157..3441
                     /locus_tag="ACG8OO_002168"
     CDS             3157..3441
                     /locus_tag="ACG8OO_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP9046027.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3474..4271
                     /locus_tag="ACG8OO_002169"
     CDS             3474..4271
                     /locus_tag="ACG8OO_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9046028.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4285..5118
                     /locus_tag="ACG8OO_002170"
     CDS             4285..5118
                     /locus_tag="ACG8OO_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9046029.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5136..5993
                     /locus_tag="ACG8OO_002171"
     CDS             5136..5993
                     /locus_tag="ACG8OO_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9046030.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6015..6524
                     /locus_tag="ACG8OO_002172"
     CDS             6015..6524
                     /locus_tag="ACG8OO_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9046031.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6561..6962
                     /locus_tag="ACG8OO_002173"
     CDS             6561..6962
                     /locus_tag="ACG8OO_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046032.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7074..7427
                     /locus_tag="ACG8OO_002174"
     CDS             7074..7427
                     /locus_tag="ACG8OO_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046033.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7461..7904
                     /locus_tag="ACG8OO_002175"
     CDS             7461..7904
                     /locus_tag="ACG8OO_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9046034.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7921..9006
                     /gene="uxuA"
                     /locus_tag="ACG8OO_002176"
     CDS             7921..9006
                     /gene="uxuA"
                     /locus_tag="ACG8OO_002176"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9046035.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8999..9712
                     /locus_tag="ACG8OO_002177"
     CDS             8999..9712
                     /locus_tag="ACG8OO_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9046036.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9919..10068)
                     /locus_tag="ACG8OO_002178"
     CDS             complement(9919..10068)
                     /locus_tag="ACG8OO_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046037.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10464..10664
                     /locus_tag="ACG8OO_002179"
     CDS             10464..10664
                     /locus_tag="ACG8OO_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9046038.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3589 a         1696 c         2320 g         3121 t
ORIGIN      
        1 acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat
       61 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
      121 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      181 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      241 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      301 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      361 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      421 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      481 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      541 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      601 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      661 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      721 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      781 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      841 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      901 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      961 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
     1021 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1081 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1141 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1201 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1261 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1321 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1381 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1441 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1501 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1561 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1621 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1681 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1741 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1801 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1861 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1921 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1981 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     2041 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2101 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2161 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2221 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2281 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2341 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2401 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2461 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2521 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2581 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2641 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2701 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2761 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2821 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2881 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2941 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     3001 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3061 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3121 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3181 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3241 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3301 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3361 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3421 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3481 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3541 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3601 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3661 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3721 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3781 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3841 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3901 tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct atgcatgacg
     3961 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     4021 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4081 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4141 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4201 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4261 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4321 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4381 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4441 gcgatgattg ttacagccgc tttgcaaatg atttatatgg ggggtttttc tccaggagga
     4501 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4561 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4621 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4681 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4741 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4801 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4861 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4921 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4981 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     5041 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5101 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5161 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5221 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5281 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5341 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5401 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5461 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5521 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5581 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5641 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5701 tgcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5761 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5821 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5881 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5941 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     6001 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6061 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6121 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6181 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6241 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6301 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6361 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6421 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6481 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6541 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6601 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6661 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6721 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6781 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6841 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6901 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6961 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     7021 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7081 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7141 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7201 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7261 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7321 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7381 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7441 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7501 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7561 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7621 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7681 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7741 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7801 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7861 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7921 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7981 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     8041 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8101 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8161 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8221 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8281 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8341 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8401 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8461 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8521 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8581 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8641 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8701 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8761 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8821 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8881 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8941 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     9001 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9061 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9121 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9181 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9241 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9301 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9361 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9421 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9481 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9541 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9601 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9661 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9721 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9781 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9841 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9901 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9961 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
    10021 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10081 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10141 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10201 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    10261 ttcgaagaca atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag
    10321 aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct
    10381 acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga
    10441 tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca
    10501 aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta
    10561 tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag
    10621 atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta
    10681 accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa aaattc
//
LOCUS       DAZKBH010000089        10540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000089 DAZKBH010000000
VERSION     DAZKBH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.090"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8OO_002180"
     CDS             33..656
                     /locus_tag="ACG8OO_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046039.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8OO_002181"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8OO_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9046040.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8OO_002182"
     CDS             2152..3033
                     /locus_tag="ACG8OO_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9046041.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8OO_002183"
     CDS             3036..3806
                     /locus_tag="ACG8OO_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9046042.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8OO_002184"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8OO_002184"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9046043.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8OO_002185"
     CDS             5117..6280
                     /locus_tag="ACG8OO_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9046044.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8OO_002186"
     CDS             6397..6807
                     /locus_tag="ACG8OO_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9046045.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8OO_002187"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8OO_002187"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9046046.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG8OO_002188"
     CDS             9380..9664
                     /locus_tag="ACG8OO_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046047.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG8OO_002189"
     CDS             9824..10060
                     /locus_tag="ACG8OO_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9046048.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG8OO_002190"
     CDS             10057..10455
                     /locus_tag="ACG8OO_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP9046049.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3676 a         1769 c         2230 g         2865 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag
//
LOCUS       DAZKBH010000090        10504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000090 DAZKBH010000000
VERSION     DAZKBH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.091"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..304
                     /locus_tag="ACG8OO_002191"
     CDS             26..304
                     /locus_tag="ACG8OO_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046050.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            323..1531
                     /locus_tag="ACG8OO_002192"
     CDS             323..1531
                     /locus_tag="ACG8OO_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9046051.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1679..2044)
                     /locus_tag="ACG8OO_002193"
     CDS             complement(1679..2044)
                     /locus_tag="ACG8OO_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFP9046052.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(2191..2964)
                     /locus_tag="ACG8OO_002194"
     CDS             complement(2191..2964)
                     /locus_tag="ACG8OO_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9046053.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3138..3998)
                     /locus_tag="ACG8OO_002195"
     CDS             complement(3138..3998)
                     /locus_tag="ACG8OO_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9046054.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(3998..5416)
                     /locus_tag="ACG8OO_002196"
     CDS             complement(3998..5416)
                     /locus_tag="ACG8OO_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9046055.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(5473..5922)
                     /locus_tag="ACG8OO_002197"
     CDS             complement(5473..5922)
                     /locus_tag="ACG8OO_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9046056.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(6367..6630)
                     /locus_tag="ACG8OO_002198"
     CDS             complement(6367..6630)
                     /locus_tag="ACG8OO_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9046057.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6729..8024)
                     /gene="rho"
                     /locus_tag="ACG8OO_002199"
     CDS             complement(6729..8024)
                     /gene="rho"
                     /locus_tag="ACG8OO_002199"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9046058.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8021..9292)
                     /locus_tag="ACG8OO_002200"
     CDS             complement(8021..9292)
                     /locus_tag="ACG8OO_002200"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9046059.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(9457..10326)
                     /locus_tag="ACG8OO_002201"
     CDS             complement(9457..10326)
                     /locus_tag="ACG8OO_002201"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9046060.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT         2937 a         2224 c         1845 g         3498 t
ORIGIN      
        1 ttttttaaga atagagggaa aaaacatgaa ttactggatg aacacaatca ttaatcgatt
       61 agaaacagca tatcaaacaa gatttgatat gaaagcttct ttagtctttt taaatgatgc
      121 gtatcaaaac tcgatcgaat tgatcaaagc agtggatgag aatccaacaa acgaatgcga
      181 agaattttta aatctgttta tgtctacaag agatttattt attcgccaac ttgttgatcg
      241 ctatccatcc aattatcacg atgtagaagt tcaaatacaa aaattaaaag catattcagc
      301 ttaaggggaa tgtataacat acatggaaaa gaaattattt aataaatatt ttattggaat
      361 cacattgatc aattttattg tctacctgat ttattatctg ttgatggtca tcatggcagt
      421 cattgcgcaa gacaccttac acgccagctt gagccaagca ggttttgctt ctgggatcta
      481 tatcatcgga acattgctgg cacgcttatt catggggaaa cagttagaac tatttggtcg
      541 gaagtttacc ttaagaggcg gcgcgatctt ctatctcctt tcaacattag catatcttta
      601 tacgccaact atcggggcat tgtatttagt tcgattcttg aatggattcg gctacggtac
      661 agtttctaca gctacaaatg ctatcgttac tgcatatatc cctgcatcta aaaaagggga
      721 aggcatcaat tattatggat taagtactag tttagctgcc gctgttgggc catttttagg
      781 aatgattttg ctgaatatga ctgattttcg tttcattatc tggttttcaa ttgtactggt
      841 cttctttgtt acgattgcct gccttgtctt tcctgtaaaa aatattccat tatctgaaga
      901 acaaaaaata tctcttcgct catggaatat caatagtttt attgaaaaga aagcactttt
      961 cattacagga attgcctttt tgatgggtct ttcttattca agtgttttat catttttatc
     1021 ttcttataca aaagtaatcc acttagttgc tgctgggtca tttttcttcg tcgtatatgc
     1081 tttggtcatc acatttacac gtccattgac tggaaggatt ttcgatgtca aaggagaaca
     1141 atacgtgatg tatcctagct atttattctt aacagcagga ttatttttac tgagtgtaac
     1201 gacgaatagt gtgacattgc ttatctctgg agcattagtc ggcctaggtt atggtacgtt
     1261 tatgtcaaat ggacaggctg tttgtttaaa aatcgtccgc gaacatcgaa tcagtattgc
     1321 tttatctacc tactttattg gtttagacct aggcttaggc gttggtcctt atttgatggg
     1381 gatgctaaaa tctcttactt catttagagg attgtatgtg attgccggag tcattccatt
     1441 gatatgtaca gcactctatc ttgttaatgg ccgaaaacat agtcatgaag cagagggcga
     1501 attgattgaa tcattgaaaa atgaaatttg atcttgatga atagataaaa aaaaccgttc
     1561 agatctgaaa ataatctgag cggttttttg agtaaaaata gtttatgatt attcttaatt
     1621 aaacatctat ttatttttaa agctttcatt tatcgataag aatagtttaa ctgttgattt
     1681 attccatttt ccgttcatct ggtacgatgc cttgacttag caaccacttg tggaagaacc
     1741 attctgccac accgatcaat agagcaggaa tcaatacttt catccaaata tcgactgtat
     1801 cattcataaa atatagatag agccacaaga taactgcagc tgaaccagca tctgcgatcg
     1861 tcgctgcgat gttccctact ttacgtaaga tcaacagatc ccctagcaca tagatcatga
     1921 ctacaataat taccccaaca atcgttgaat ccataaagct aacaccgtat aaacctgtca
     1981 gcacgaccca cagaatcgcc cagatcatga ttgccttcac taccaacgcc ttcacatgtt
     2041 tcattttctg cactcctttc tcctggataa aaaagtaaga ttatctaaca agataacctt
     2101 ttcattatta taccatcggc tgagagaagc gagcaaagat aaggtcctat ctaaaaatga
     2161 ggaagccact tagaacattc acgtaataat ttatccgtac agacattcga tatggtgttc
     2221 ctttactttc gcatactgtt cttttgtcag ttctttactt gtcaccagat aatcgatttc
     2281 atgaaaagca atggcttgat gcatgaattt atgtgcaatt ttttgctgtg taactaaaac
     2341 ggcaagccgg tctgattgtt ttctaaaagc ttttttgacc attgcctctc cttcatgagt
     2401 ttctgttact ccttggctga aatctagccc gcgacaactc attatcgcta aatccgcata
     2461 atatgacaat gcatcgttat aggcttttga accgttgatt gttgctcgtt tcggaacaat
     2521 tgcccctccc aatacagata cttttgccgt tgtatattca ctcaacatag cagctgtgac
     2581 gatgccattc gtaatgatct gcaaaccatc aaatcttgct aggtttctga ctaagtgtaa
     2641 agaggttgta ctggaatcca agaataagac catatcatct cgaatcaaag aagaagctgt
     2701 atctgcaatc ttacgttttt ctgtttcttc ttctaatttg gtgatcttca tcaggtaagg
     2761 cacgtcaatt tttcgattgt caataagcat ggcgccaccg cgaactctac ggatcaatcc
     2821 acttttttcc agaaaaacaa ggtcacgttt aatcgtggct tcgctataat ttactaactc
     2881 gatcaaatcg gtgactcttg caaattggtt gttttttaga taattcacaa tctcctgctg
     2941 tctattctcc acttcattca acataaaatc accccattta ctttatatag taacaggaaa
     3001 aaagaggttg tgacagaagt gattggcttc gagaaataag aagaaatttt tcgaaaaaac
     3061 tacttctgaa catcgtgtgt ggttaagagg gtatgacaag actattgtca taccctcttg
     3121 accacacatt tttattttta gtataaacta gccttgccta ctgtgttcaa aatttccatt
     3181 ttatgtttga ccacttcttg taccttttta ttagctgctg gataaattgc attcggctcg
     3241 tacatcgatg gattttcact caatatttct ctcaaagcat tgaaaaagac gcttttaaga
     3301 tctgagctca aattcacttt ggcaactccg tattttactg cttcgctgac ttctgcgtcc
     3361 ggattacctg aaccgccatg taaaacaaat ggaagatcga tttgttggtt caattctttc
     3421 agtaagtcga tatttaattt tggagttctg tttttcggat acaagccatg cgctgttcca
     3481 attgctacag caagtgtatc gattcctgtt tctttcacaa aaatcttcgc ttgatcagga
     3541 tctgtataaa tgattgtttc agcacctcct tcagcagaac cattattccc aatcgttcca
     3601 agctcagcct ccactgagac atttaccgca tgagctaacg caaccacttt tttcgtaatc
     3661 tgtacattgt cgtcgaatga tttcaaagac gcatcgatca tcacggaaga atagccgttt
     3721 cgaatcgccc gtaagacatc ttctatcgta ccgccatgat ccaagtggat aacgatcggc
     3781 agtttacttg tataagcata ggctcttact gccgcaacaa actcatcact taaataggcg
     3841 atttcatcag gatgaatttc taagattact ggtgcttcta atctctctgc ttcttcgatg
     3901 attgctttca tcatatcata gctgcaaata ttgaaggctg gtactgcaaa ttgattctct
     3961 ttagccactt tcaataactc tttcattgta tataacatta cgcttccacc tcatttgtta
     4021 gattttcatt tttcgtccac ttgtcttgtt ttttctcttt cactgttttt ttcaatagtc
     4081 caagcataaa ggctgtagtg actgtacctg atgcaattgc caagacatac ataggtaaat
     4141 tgttcataac atttggaatt agcatcaccc aaatccctcc atgaggtgcc attgaagtaa
     4201 ctcctactgc catggaaatc cctgcagcga ctgctgaacc tgtcatcaaa ctaggtatca
     4261 cgcggatcgg gtcagatacc gcaaacggta tagcaccttc tgtaatatac gaagcgccta
     4321 aaatccagca tgattgacct gaagtccgtt cttctttggt aaatttatgt ttgaataata
     4381 aagtggctaa agcaatacct aggggaggtg tcattcctgc agccatacaa gcagcaatcg
     4441 gtgcatttac tccagctgac atcaaaccga tcgagaaagt cgaagtcgat ttattgatcg
     4501 gcccacccat atcagctgcc atcatgatcc cgataagtaa tccgaacaat agcccggatg
     4561 tatttcccaa gctattcagc caatttgtca aagcatccaa cacccatgtg atcggttgtt
     4621 caatcacaaa taccatcaaa agcccaataa ttaagatcga taaaaagggg acgacaatca
     4681 gctgataaat agcagaaaat tcttttttta cgctaatttt atttgccaga aaatcacaga
     4741 tataccctgc tagcacgcca ccaatcattc cgccaagaaa acctgaacca cctgtagagg
     4801 ccagtaatcc ggcaacaatt cctggggcaa aacctgcttt tccagcaatc gatgtagcaa
     4861 tccccgcgcc taacgcagga accatcatgg caaacgctgt atctcctccg atccttgaaa
     4921 atgcagctgc taacacatta tagtctgctg agttgggatc agccgctgta atcccaaagg
     4981 caaaactcag tgcgatgatg attccgcctg cgatcacaaa tggaagcatg taattcacac
     5041 cattcatgaa atgattatag attgtcggct ttttctcatt tgcagctggt tctttttcat
     5101 tagacacaac gaatacgcca atatctctca atgcttcgtc tattactact tgcggttctt
     5161 tgattgctcg gcttgtagag acacgcttta cttttttccc ggcaaatcgg tccaattcga
     5221 tttctttatc tactgcaaga atgattgcat ctgcctcttt gatttcttgt tcagataact
     5281 gattttctgt ttttacacct tgtgtttcga attttgcttg atggtttaga tttcttgctg
     5341 ttacttctaa tttttctgcc gacatataag tatgagcaat tccagtcggg caagcagtta
     5401 caccaacgat tttcatgatt attttcctcc tttttcttca ctatcgatac tcggacaaag
     5461 actctactat tttcattctt gactaatctg tctgatgata tctgctggcg tagtggcttc
     5521 ttttattccc tttctaaaat ctgcattcat caatcgtctg gagagtgtgc tgagaatctc
     5581 tagatgcata tctgctgatt tttcaggaac tacgatggca aaaatatatt caattggcat
     5641 ttcatctatc ccgctatagt ccgttaattt ggctgtatga aaaaaacaca gtgctggttt
     5701 tgtcacatat ttgctttttg catggggaat cccaaatcca tcttctattc ctgtggagat
     5761 ctcctcctct cgtttgaata gtgccttaac gactttttga acatttctta ccttatttgt
     5821 ttttttcaac acatccccta ttcgctcaaa taatgatcct tttgaatcta tcgatggatc
     5881 gaaaacaatc agttcttctg cgatcaattg atcaaacctc ataaaaaacc tctcccccac
     5941 aaaaaatgtt aacgttttct tggaactgag gtcagtatag catcgctagc tgtaccgttc
     6001 aatattatga atgaacagag gaaaaagacc catcatccgc gtgtataagt cattaaaatg
     6061 aacgatctag ctcacaaaca atcgcaagtc tataacaaaa caaaaagcct tccttgaaag
     6121 gaaagcttag cttctaattt taggtaaatg gttttttact tcgaaataac ccctcttcat
     6181 ttcgttaata taaggtgttt ttatcaagtg gataattaaa actatatact agtttttcat
     6241 tgttttgtgc attatgatcc tacgactttt tcagcatcaa acgaagtttt aaattggcaa
     6301 aaaacataaa aaaacagact atccctatca gatagtctgc taaacgttga gttatcagcg
     6361 tttttattat tccgcagcgt ttgcgtcttt gagaccgtat tttttgttga aacggtccac
     6421 acgtccgtct gcttgtgtga atttttgacg tccagtgtag aatggatgtg agtcagaagt
     6481 tacttcgaca cggatgactg ggtatgtatt accgtcttcc cattcaactg tttcttgtga
     6541 agtttttgtt gaacctgaca agaatttgaa accagttgtt gaatccatga atactactgg
     6601 gtggtaatct ggatggatgt tttctttcat gattttttcg ctcctttgcc ctgctccatt
     6661 tgctgaacca gagttgattt gtcgatttac aacattaaca tcttaacatg tttcagtcaa
     6721 ggttgcaatt atcttttcag atttctggtc ggattttttt taccaaagga gacatcttga
     6781 aattctttga aaaattgctg attgtttttt gttttacgca aaaagcggat cagttgttcc
     6841 gtatattcta atgaatctcc agtcatattt tttcgaagtt tccaaatttc ttctaattct
     6901 tcacttggca tcaataattc ttcttttcgg gtacctgatt ttttgatatc gatggctggg
     6961 aagatccgac gttcagataa ttcacgtgaa agttgtaatt ctagatttcc agttcctttg
     7021 aattcttcat agatcacgtc atccatacgg cttcctgtat cgactaaagc ggtcgctaaa
     7081 atcgtcaaac tgccaccttc ttcgatgttt cttgctgccc cgaaaaattt cttaggttta
     7141 tacaatgcgg ctgggtcaat cccgccactc aatgtacgtc cgcttggtgg aacgactaag
     7201 ttataggccc gagcaagtct agtaatacta tccatcaaga taacgacatc tcgtttatct
     7261 tcgactaaac gcattgcgcg ttccaatact aattcagaaa ctcgagtatg gttttgcggc
     7321 tgcagatcaa atgtagaaga taccacgtcg cctttcacgc ttcgttctag atccgttact
     7381 tcttccggtc gttcatcaat cagtagaacg attaattcta cttccggata gttttcggtg
     7441 ataccatttg cgatttcttt taatacagaa gttttccctg cttttggcgg tgcgacgatc
     7501 aatccacgct gaccaaaacc gattggagaa aagatatcga tcattcgggt agatagtctt
     7561 tgcggtgttg tttccagttg aagctgtctt tgcggataaa gaggtgttaa ggctggaaaa
     7621 tgcggacgtt cttttgcttc ttccggatct tttccattaa cgctttccac gtgcatcaaa
     7681 ccataataac gttccgattc tttaggtgga cgggcttttc ctgccacttt gtccccattt
     7741 ctcaaactga atcgtctgat ttgtgaggca gaaatataga tatcttctgc acttggtcca
     7801 taattgattg gccgtaaaaa accgtaacca tcttgaccaa cgatatccaa tattccttcc
     7861 atataataaa atccttgttt ctctgcttgt gcacgaatca cagctagaga taattccttt
     7921 ttattcatct tactgtaata aggaatttta aattctttgg cataactata aacttctttt
     7981 aacgttttgt tctctagttc tgccattgtc aaataatcgc tcattcagct cccacttcct
     8041 ctatcatttc aattgatgcc cctaaggctg ttagtttctc gataatatga tcataaccgc
     8101 gtaagatata atctacattg taaatcgtcg ttgtcccttc tgccaatagt ccagcaatca
     8161 ctaaacaagc acctgcccga agatcagaag ccacgacttc tgtgccgtga agtttatttg
     8221 gaccattcac gatgatcata tttccttcca cggtaatatc agcgcccatt cgtgctagtt
     8281 ccggcacatg gttcacacgt ttcgcataga tcgtatcgat gatttctgaa gtgcctgtag
     8341 tcatcaatag taacgcagta agtggctgct gcagatctgt ggcaaatcct ggataaggat
     8401 aggtcatgat gtttgcgggt tttaattctt tcgaggggta aacttcgatt tcatcttcgc
     8461 tgattttcat atggacacca atttcctgca attttgctat aaagctttcc aaatgttcaa
     8521 agatcacatt tttcactttg ataccatttc ctactgcagc tgctaatgct agataggttc
     8581 ctgcttcgat gcggtcagga atcatgaaat gccgacagcc atgtaatgtt tcgactcctt
     8641 cgatacggat cacatctgta cccgctcctc gaacttttgc ccccatatta ttcagtaaag
     8701 ttgccacatc tatgatttcc ggttctcgtg cagcgttttc aattactgtc tgtccttttg
     8761 cttttacagc agctaacatc acattgatcg ttgctccgac tgaaaccacg tccatgaaaa
     8821 tccgattgcc acgcaatcct ttattttctg tccgtaaata catggcacca tgttcatttg
     8881 tcacttcagc acctagagct tcaaatcctt tgacgtgtag gtcaatcggt ctagggccta
     8941 aataacagcc acctggcagg cctactaccg cttcgccaaa tttaccaagc agtgttccca
     9001 taaaataata agaagcacgc aaactattga tttttccgtg cggcatcgga atcgatacga
     9061 cttctcgagg atcgatttct agtatgttgt tggaaaaatg gacagttgcg cccatgatct
     9121 ctaaaatttc aattaatgaa tgaacatctt gtatgtccgg cacaccttcc aagatcactg
     9181 gggaatcagc taagattgca gctggaatca atgcgactgc actatttttt gcaccgctga
     9241 tcgttacttc accagctaaa ggtcggtttc ctttgattac gatttttttc atctgtttcc
     9301 accttgttta ttaatctttt ttcatggata tgcttgtatt ttatcataga taaaaaaaag
     9361 aaacgagcat ggtctgccgt tttaagtaaa aaagaatgga gagcgacttc aaaagtcgtt
     9421 ctccattcct atatatcgta tctgttatgt ggatgcttac gctttgttag ctgaaccgaa
     9481 ccagtcgata tgagatttag catcttcaac gattgctgct ttacctggag ctaataattt
     9541 acgagggtcg aatcctttac cttctttatc tttaccagct tcgatatatt tacgagtagc
     9601 tgcagcaaat gataattgga attcagtgtt aacatttact tttgagatac ccattgagat
     9661 agctttttcg atttgatctt gaggaatacc tgatccgcca tgtaatacta aaggcatgtc
     9721 tggacctaca gcatcagcaa ttgcttgtaa gtggtcaaaa gctaatcctt tccagttttc
     9781 tggatattga ccatggatgt taccgatacc gcaagctaag tagtcgatac ctgtagcaac
     9841 catttgtttg cattcttcaa tatcagctaa ttcgccgtta ccgatgattc cgtcttcttc
     9901 tccgccgatt gaaccaactt cacattcaac tgagatacct tttgcatgtg ctttttcaac
     9961 aacgtcttta gctaatttca agttttcttc aaatggtaga tgtgaaccat cgaacattac
    10021 tgaagtatag cctaattcga tacattccaa agcagcttcg taatcaccat ggtctaagtg
    10081 gatagcaact ggaacagtga tgttcattgc ttcaactaaa tcagtgatta aatctttaca
    10141 gattttgtaa ccacccatgt atttagctgc acccattgat gtttggataa gtactggtgc
    10201 ttgttttgct tcagccgctt caaggattgc ttgagtccat tccaagttgt ttgtgttgaa
    10261 tccgccgaca gcgtaaccgc ctttacgagc agcttttaaa aattcagctc ctgatactac
    10321 tggcataata aaattcctcc taagttttgt aaaactaaat ttgttaagac aattcttaac
    10381 caacgaccct attttaccaa acatttcggt agttttctac taaaatcaat gaaaatgaaa
    10441 attttcaaga agttttttgc aaaaaataaa atatccaata gcgctttaat cgaaaaaagt
    10501 cttt
//
LOCUS       DAZKBH010000091        10194 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000091 DAZKBH010000000
VERSION     DAZKBH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.092"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..627
                     /locus_tag="ACG8OO_002202"
     CDS             121..627
                     /locus_tag="ACG8OO_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP9046061.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            644..1543
                     /locus_tag="ACG8OO_002203"
     CDS             644..1543
                     /locus_tag="ACG8OO_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFP9046062.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1630..2514
                     /locus_tag="ACG8OO_002204"
     CDS             1630..2514
                     /locus_tag="ACG8OO_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9046063.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2706..2777)
                     /locus_tag="ACG8OO_002205"
     tRNA            complement(2706..2777)
                     /locus_tag="ACG8OO_002205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2742..2744),aa:Arg,seq:ccg)
     gene            2837..4171
                     /gene="rpoN"
                     /locus_tag="ACG8OO_002206"
     CDS             2837..4171
                     /gene="rpoN"
                     /locus_tag="ACG8OO_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9046064.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4320..5411
                     /locus_tag="ACG8OO_002207"
     CDS             4320..5411
                     /locus_tag="ACG8OO_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFP9046065.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5452..6453
                     /gene="gap"
                     /locus_tag="ACG8OO_002208"
     CDS             5452..6453
                     /gene="gap"
                     /locus_tag="ACG8OO_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9046066.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6563..7753
                     /gene="pgk"
                     /locus_tag="ACG8OO_002209"
     CDS             6563..7753
                     /gene="pgk"
                     /locus_tag="ACG8OO_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9046067.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7857..8612
                     /gene="tpiA"
                     /locus_tag="ACG8OO_002210"
     CDS             7857..8612
                     /gene="tpiA"
                     /locus_tag="ACG8OO_002210"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9046068.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8728..10026
                     /gene="eno"
                     /locus_tag="ACG8OO_002211"
     CDS             8728..10026
                     /gene="eno"
                     /locus_tag="ACG8OO_002211"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9046069.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2631 t
ORIGIN      
        1 tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa
       61 aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga
      121 atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat
      181 ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat
      241 aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa
      301 aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca
      361 ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc
      421 ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata
      481 gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa
      541 attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg
      601 aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga
      661 aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga
      721 ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa
      781 gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct
      841 tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat
      901 tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc
      961 gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata
     1021 ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac
     1081 ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt
     1141 gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac
     1201 aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta
     1261 tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc
     1321 atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact
     1381 tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc
     1441 tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt
     1501 gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga
     1561 ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga
     1621 agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg
     1681 tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct
     1741 tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca
     1801 actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag
     1861 tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc
     1921 gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta
     1981 aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct
     2041 gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat
     2101 cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact
     2161 gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc
     2221 acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa
     2281 gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat
     2341 ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg
     2401 atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat
     2461 ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga
     2521 caagtatctt taataaataa cacattttaa taagtagttg aacacataac taccttactt
     2581 gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg
     2641 cggacagttt tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag
     2701 tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc
     2761 cactacacta aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag
     2821 gggattggac aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca
     2881 gaaactagcc atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga
     2941 ggagctgttg gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga
     3001 gccggaatgg cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac
     3061 aaattaccta aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca
     3121 agtccatttg aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata
     3181 catcgatttg aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc
     3241 tacgcctatc caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt
     3301 aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc
     3361 ggaattagct tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa
     3421 agaaatcgca caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt
     3481 acaaacgttg agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat
     3541 tcctgatgtc cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa
     3601 aaatcagcca aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga
     3661 taaagaaact gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac
     3721 gatcctacaa cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa
     3781 agctttcttt atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac
     3841 tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac
     3901 agacttcggt gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac
     3961 agaacagaca gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga
     4021 tcaagaagac aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga
     4081 tgagatagcg atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag
     4141 ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca
     4201 ctttttctag tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag
     4261 gttgagattt ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata
     4321 tgggtcgttt taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg
     4381 atgaaataaa aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct
     4441 ataaaatttt acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa
     4501 gtatggggat cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt
     4561 tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa
     4621 gccttgaaaa cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag
     4681 aatattttgg taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag
     4741 tggctgatgc attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg
     4801 agaatatcat cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta
     4861 atctggaaaa taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag
     4921 cgataactgt acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc
     4981 accgtgcact ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg
     5041 agccatctat ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta
     5101 ttgggcgtgc attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc
     5161 tgaaacaagc aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag
     5221 tcgtttataa ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca
     5281 tcatggcaat cgcgggcgga aaatcaaaag ccaaagcaat tcgagcttat atgaaaaatg
     5341 caccaaaaca aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg
     5401 taacccttta aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt
     5461 aaagtaggta ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat
     5521 gtagatggaa tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac
     5581 ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt
     5641 tcattcaacg taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta
     5701 ccatggggcg aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa
     5761 tcagcagctg aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc
     5821 ggaaacgatg ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa
     5881 acagttatct caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg
     5941 aacgacaaat ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac
     6001 caaatgactc tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct
     6061 gcaaacatcg ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa
     6121 ttgaacggta aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca
     6181 gaattagtaa cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa
     6241 gaagcttcaa acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt
     6301 ggtatgactt acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac
     6361 aaacaattag ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta
     6421 gtacgtactt tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta
     6481 tagacatgta catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata
     6541 agtttattca ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga
     6601 caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga
     6661 cgacactcgt atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa
     6721 agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc
     6781 tttagctcca gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc
     6841 tgaaactcgc ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt
     6901 cgtattcgaa aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac
     6961 agaactaggt aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac
     7021 agctcaccgt gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc
     7081 tggattcttg atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg
     7141 accaatgatt gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa
     7201 cttgattcct aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga
     7261 agcaaaagga atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa
     7321 agaattaatc gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc
     7381 agaattttca aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat
     7441 ggctttagac atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa
     7501 aacagttgtt tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac
     7561 aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg
     7621 agactcagcc gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac
     7681 aggtggcggc gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat
     7741 caacgataaa taaacgacca agtttgctca attctgacga gtaatcgatc aaaaggtgaa
     7801 gggagttttc atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc
     7861 gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat
     7921 ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat
     7981 ctcctgcatt attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct
     8041 cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag
     8101 ctcttgctga tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt
     8161 tccatgaaac agacgaagac atcaataaaa aagcgaaagc tatctttgca aatggtatga
     8221 caccaatcct atgctgtggc gaatctttag aaacctatga agcaggcaaa actgctgaat
     8281 ggatcgaagg acaaattaca gctggtttag ctgatctttc agctgaacaa gtgtcaaaca
     8341 tggttattgc ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa
     8401 tcgctgacga aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag
     8461 tatcagaagc agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat
     8521 acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt
     8581 cattcttgtc attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga
     8641 aatgtttgca ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa
     8701 aaaacaaact caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag
     8761 atcttagact cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct
     8821 tttggccgcg gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta
     8881 cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta
     8941 aacaacatca ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac
     9001 aaagctatga tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt
     9061 ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac
     9121 cactacctag gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac
     9181 ggcggatcac atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc
     9241 gctccaacat tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca
     9301 atcttgaaag ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac
     9361 cttggttcaa acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat
     9421 gtacctggta aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa
     9481 gaaaaaggtg tttacgtact agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg
     9541 atcaaattct acgaagaatt agtttctaaa tatccaatca tctcaatcga agacggatta
     9601 gacgaaaacg actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa
     9661 ttagttggtg acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa
     9721 ggaattgcta actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc
     9781 gaagctatcg aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt
     9841 gaaacagaag attcaacaat ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa
     9901 actggttctc tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa
     9961 gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac
    10021 aaataattct tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc
    10081 acttagttaa gtaatcgaac tctctacttg ctgaaatatc ggctctttgt caataaggac
    10141 tgatgagttg tgcaaaatca aatctgagtc agaatgaacc acattctggc tcag
//
LOCUS       DAZKBH010000092        10159 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000092 DAZKBH010000000
VERSION     DAZKBH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.093"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..464
                     /locus_tag="ACG8OO_002212"
     CDS             144..464
                     /locus_tag="ACG8OO_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046070.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            917..1117
                     /locus_tag="ACG8OO_002213"
     CDS             917..1117
                     /locus_tag="ACG8OO_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9046071.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1264..4053
                     /locus_tag="ACG8OO_002214"
     CDS             1264..4053
                     /locus_tag="ACG8OO_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFP9046072.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4100..4627
                     /locus_tag="ACG8OO_002215"
     CDS             4100..4627
                     /locus_tag="ACG8OO_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFP9046073.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4648..5838
                     /locus_tag="ACG8OO_002216"
     CDS             4648..5838
                     /locus_tag="ACG8OO_002216"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9046074.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5878..7176
                     /gene="asnS"
                     /locus_tag="ACG8OO_002217"
     CDS             5878..7176
                     /gene="asnS"
                     /locus_tag="ACG8OO_002217"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9046075.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8352..9026
                     /locus_tag="ACG8OO_002218"
     CDS             8352..9026
                     /locus_tag="ACG8OO_002218"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9046076.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            9023..9364
                     /locus_tag="ACG8OO_002219"
     CDS             9023..9364
                     /locus_tag="ACG8OO_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046077.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9394..9753
                     /locus_tag="ACG8OO_002220"
     CDS             9394..9753
                     /locus_tag="ACG8OO_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9046078.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
BASE COUNT         3394 a         1813 c         2174 g         2778 t
ORIGIN      
        1 tttaaaaaag aggttgtgac gtggtcacga cctctttttg acgattatga tacaaaaaac
       61 atcatttatg aacaaaatgt gaataacggg cgttcttgag atatgatttt aattgggaaa
      121 aacttatcga taaggaggtc tttatgaaaa aaagactgtt atggtgtttg ctgtctctat
      181 ccctaagttt aacaagtcag ccagtttttg ctgaagaaac acagcggttt cctattacag
      241 gcacacgtct gtacaaaaat gtttatgtac agacaccaga tcttgattct gcgattcctg
      301 aaacagaaga acggtctttg cagccaagat ttggttactt ttggtactgt ccgacctgtg
      361 attatgaaag ttgctggcat ttcttacatt ctacagctgc agcttttgct tttactcata
      421 ctcagaaata tgatggacat catcctgtta tttatgaggt atagatatga gcaaaaaaga
      481 aaaactaatc tcgttggtcg tattggtttg ccttgttctt ctggtaactg tcataggcta
      541 tcagaaatac catgagaaca tcagacctac taaagaatct tccccagcct cgaccgttga
      601 aacagaaccg ataagtagtc agaaagaata atttttttag tttaaaaaag aacatcagag
      661 caaaaaaaca ccaactgaac aatatgtcgt ttattttaaa aaaaagttcg gtttttcact
      721 ttatgtaatc gctgagaaaa ctttttttaa taaaaaaagt cgaaaaactc tagctttttt
      781 gtaaacggta tgatatacta taggagtagt ttttgttcgg taatctgtgg tagcttgttt
      841 caaataaaca tcttccagat atacaccatt caagtaattg caaaatatgt gttgatacac
      901 aaggaggatt tttattatgg aaacaggtac agtaaaatgg tttaactcag aaaaaggatt
      961 tggattcatc actgcagaaa acggaaacga tgtattcgtt catttctcag ctatccaagg
     1021 cgacggattc aaaactttag aagaaggcca agcagtaact ttcgatgttg aagaaggcca
     1081 acgcggacct caagcaacaa acgttactaa agcttaatta acctatacta aaaaataaac
     1141 gcccagacat ttgcgtgtct gggcgttttt gtctattttc tttaaaaagc gatgagagta
     1201 tttttctgca ctttgtagta taatttcaaa agaacattag aggcaggaga agaggaagat
     1261 attttgaaac caagaactta tgcagtcgtt gatatcgaaa cgactgggac gaatccaaaa
     1321 gaagatcgga tcatccaatt tggctgcgtc atgattgaat caggtcggat cacttcacgt
     1381 ttttcgatcg atattcatcc agggcggaag atttcaaaac aaatccagca tttaacaggg
     1441 atcacgaatc aacgcgtcca aaaagcgcct tatttcgaag atgtcgccca aacgatttac
     1501 aatcttttgg cagacaccat atttgtcgcg cataatattt attttgatta taattttttg
     1561 aaccatgaac tcatgcgatg cggacttcct agtttgaaga taccaggcat cgatacagta
     1621 gaacttgcac aaattttctt gccgacggaa ccaagctttc gcttagctga tttgtcagaa
     1681 agccttggat ttattcatga gaatcctcat caggcagata gtgatgccga agtgacaggt
     1741 caacttcttt tgctgatcga agaacggatg agaaaattgc caatcatcac gttagagcag
     1801 attgcgcgat taagtagaca cacaggtatg gatacgagtc gtttcattta tcatgtgatt
     1861 gaagaaatga aagaaaaacc agagccactt gatccctcat tagaaatcgt cgatgggcta
     1921 gctttgcaga aaaaagaagt ggaactattc acatccgtcc attatggcga gcggacatat
     1981 ccaagaaaaa aacaagcaaa agaaaaaatg tttggtaaaa cactgatgta tcgaaaagaa
     2041 cagaacagac tgatgaatgc ggtctacgac catttcacaa aagatgaatc aaaagatttg
     2101 atgatcgaag cagctacagg aatgggtaaa acaatcggtt atctgctgcc attgagttat
     2161 ttggctacac ctgaaaaacc cgctgtcatc agtaccgttt cgctagtttt gcagcagcag
     2221 atcatagaaa aagacattcc attgctgaat caattgctag atcagcccat acaacctgta
     2281 attatcaaaa gctatcgcca ctatattgat ttgcagcgtt ttaaaggcac acttgtggaa
     2341 ccgccagaac aaaaacaata tgctttgtat caaatggcgg tcttagtttg gctgacgcaa
     2401 acgaagactg gtgatttgga tgaattacat ttaacaaatt tgaaccattc attctttgca
     2461 gatatcgcgc acagaggtat tggattttta gcaagaaatc agtcatttta cgaacaagac
     2521 tttgttcgtt tcttgcaaaa gaaaatcagg caaagtaatt ttttgattgt caatcatgcg
     2581 tttttagtac aagaaacaca gcgaaaagaa ccattgcttc cagctagtcc atttttgttg
     2641 atcgatgaag tacatcattt gccggaaatc gctgaaaaag taaatggcaa acgattaagt
     2701 attaactatt tccataaaca aatccagcaa ttagaagaac caaatcagct gtttgaccga
     2761 atacaaagct atttaccgaa agaacatgaa gccttacgct ggttatcgat ttatcgagaa
     2821 gaactttctg cgctatgcga agcacagatt tgtatggcag aaggattaat gagtttagtg
     2881 agcggcaagc aacctaacgg acagtatcca gaagaagtca tgatgacaaa agagatgttg
     2941 gatcagctgc cactggaatc tgggacagcc attgatcacc tgcagctttt ttatcaggag
     3001 atccagcagc tacagtcaca aatgcgtctg acttttgaga ttatccaaga aagctggaca
     3061 aaccagatgc ggatcgattt agcaagttta tatagcctat ttgatcaatt agacaagcaa
     3121 tacttgctga tggatcaatg gctggaagac tggcgagatc atttgatcca ttggttcgtt
     3181 cctgggaaca cagcgcagca gatgattttt catctccatg attttcaagc ggctatgttg
     3241 ccgtcgactg tttggtatcc tcgatatgat cgtattcttt atattggagg aacgctgaag
     3301 atcggcccta atcgacacta tctctctaag aggcttggca ttgaagcagc acctcttaaa
     3361 gtgattgcct ctccttatga ttatgagaaa caggcaagga tctatgtgcc aaaagaggca
     3421 cctgccgtcc atcaagtcag caatcaagca tatatcgatt atttagagac agcacttaca
     3481 ggtctgatcg aacaagaaga tcggccaata cttgtgttat tcacttctca tgatgttttg
     3541 cagaaggtct atcagaaaat gcatctgtct atgctagaaa aaggacgaga aatactagct
     3601 caagggtttg gtggaagccg ggagaagctt ttgaaacgct tcattttgtc agaaaaaagc
     3661 gtattgtttg gcgctgacag cttttgggaa ggtgtagatt tgcctggaga tgctttgcag
     3721 atcgttgtag tcacacgttt acccttcgat aatccgcttc gcccgatggt caaagctagg
     3781 aacgcttatt tagaatcaga ggggatcaat cctttttacc aggaatccgt acctaaagca
     3841 gctttgaagt tacgccaagc attaggtcgc ttgataagga cggagacaga tcgtggcgta
     3901 ttgcttattt tggatcgaag actagtcact gcaaaatatg gcgcacggat catccatgca
     3961 ttaccgaaaa aagtaccgat caaagagcta gctatggagg aaatctatca ggatatccat
     4021 gaattcttaa agaaaaatga agaaggaagt tagaggagct gtcttttttc ataattaaaa
     4081 tctgctataa tgaaaaggaa tgaagataaa ggagcgacga cccttgaaga aaaaacagac
     4141 aaaggaaaaa acgattaatc gcgtgttact tggcttgatc gtatttttat tagcggtcat
     4201 cgtgatcagc tcgatttttt acttgcgttc cagtcgtccg gcggtgcagg cgaagaaaga
     4261 agcagtagaa atcgcaaaga aatatgcaaa tcttgaggaa accgatcatt tttattggtt
     4321 tactcgaaag aagacatact tttcactgac aggtacaaat gataaaggaa aagaaatcgc
     4381 cgttatcatt cctaaatctg gtgaaaaagt aaaagtattg gatcaaacaa agggactgac
     4441 tgaaaacgaa gcaaaacaga caattgctgc ggcccatcct gaaatccaaa tcgaaaaagc
     4501 tgctttaggg atgtatgatg atcagcctgt gtgggaagta gtaggaaaag aaacaaatgg
     4561 gaaactgaat tattacctga ttggctttga atctggagat gaagtcaaga caatcaaggg
     4621 aatttaaaag gtaaaggaat gaatgggatg gagttatcaa aaagaacaaa acgattagaa
     4681 ccgtctgtca ctttggcagc tgcggcaaag gccaaggcat taaaagcaaa aggcagagat
     4741 gtgctgagtt tgactgtggg agaaccggat tttgcgacac cagaaaacat ccaagaagca
     4801 gcaatcgaag caatccgcaa tgggaaagct agctattata caccaacagc aggaatccca
     4861 gagcttcgac aggcaatcgt tgattatctg aagaaaaact atggacttgt ctatgaacct
     4921 tcacaaacag tcgtaacaga cggagcgaaa tttgctttgt ataccctttt ccaaacgatc
     4981 cttgacccgc aagatgaagt catcattcct gtgccttact gggtcagtta cggtgagcaa
     5041 gtaaaactgg cagaaggact tcctgtcttt gtgaaagggg aagaatccaa tagctggaaa
     5101 gttactgttg aacagctaga acaagcacgt tcagaaaaaa caaaagccgt aatcatcaat
     5161 tctccatcga atccgactgg aatgatttat agcaagaatg aactgcaggc aatcggtgaa
     5221 tgggcagtaa aaaatgatat tttgatagtt gctgatgata tttatggccg tttagtttat
     5281 aatggcaatg agtttactcc aattgcaacg atttcagaag caatcaaaaa tcagaccatc
     5341 atcatcaacg gcgtgtctaa aacatatgcg atgacaggct ggcgcattgg ttatgcggtg
     5401 ggaaatcctg tgatcatcaa tggaatgatc gctgttgctt cccaatcaac tagcaatccg
     5461 acagcagtca gtcaatatgc agctgttgaa gcattgaaag gtgagcaaga tacagtagaa
     5521 gaaatgcgga acgcctttga agaacgcctg aatacgttat atccgcttgt ggcagaactg
     5581 ccgggtgttt ctcttgaaaa gccgcaagga gctttctatc tttttcctaa tgtaaaagaa
     5641 acgctacgca tgtgtaagta tgaaaatgtc acacaatggg tagaagatct tttagaagaa
     5701 acaggggtag cgcttgtgac tggcgaaggc tttggggcac cagaaaatgt ccgcatgagt
     5761 tatgccactg atcgcgctac gttagaagaa gcggtacgca gaatcaagca atttattgag
     5821 agtaaaagtc aaaattaatc ctgaagaaat tcaaggaata taaaaggaga gtcatacgtg
     5881 gaacaaattc aaatcatcga ttcgaaaaac catgtgggag aaacagtaac aatcggcgct
     5941 tgggtcgcaa ataagcgatc aagcggaaaa atcgcatttt tacaattaag agacggaaca
     6001 gcctttttcc aaggtgtagt tgtaaaaagc gaagtgtcgg aagaagtttt ccaaacagct
     6061 aaaaatctac accaagaaac atccgtgtta gtaactggag aaatccgtga agacagtcgt
     6121 tcaaaatttg gttatgaaat cggaattaca aatgtggaag tggtaagcga gagccacgat
     6181 tatccgatca caccaaaaga acacggaaca gacttcttga tggatcatcg tcatttatgg
     6241 ctgcgttctt caagacaaca tgcgattatg cagatcagaa atgaaatcat tcgtgcaacg
     6301 tatgaattct ttaatcaaca tcattttgtc aaaattgatc cgccgatctt gactggttct
     6361 gcaccagaag gcactacgga tctattcgaa acaaattact ttgatcaaaa agcttattta
     6421 tctcaaagtg gccaattata catggaagct gctgctatgg ctttcggaaa agtcttttca
     6481 ttcggtccga ctttccgtgc tgaaaaatca aaaacacgac gtcatttgat cgaattctgg
     6541 atgatcgaac cagaaatggc tttcactcat caagaagaaa gtttagaagt acaggaacaa
     6601 tatgtagcat tcttagtaca aaatgtttta gatcattgcg aatatgcatt agatgtacta
     6661 ggacgagaca aagaagtctt aaaaacttat acaaaattgc cattccctcg tattacttac
     6721 gatgatgcaa tcgaattact gaagaaaaat ggatttgatg acatcgaatg gggcgaagac
     6781 tttggttcac ctcatgaaac gttcattgcg aattcattcg aacagcctgt ctttatccta
     6841 aactatccaa aagccatcaa gccattctac atgaaaccac atccaacaag agaagacgtg
     6901 gtaatttgtg cagatatgat cgcaccagaa ggttacggtg aaattattgg cggaagcgaa
     6961 cgggcaacag actatgattt cttgctagaa caaatccgta atcacggttt ggatgaaaaa
     7021 gaatattcat ggtatcttga tctacgtaaa tacggttctg ttccgcactc tggttttggt
     7081 ctaggccttg aacgaacagt cacttggatt gctggtatcg accatgtacg tgaagctatt
     7141 ccattcccac gtttgttgaa ccgaatttat ccataagagg tgatgaaaaa gctgttctgc
     7201 atcaagtaat tagaacagcc aagcaaaaac agctgttcat gtttttgaat tggcgggact
     7261 tattaccgca gatgtaagct tttgaatacc gtttagtgga gaggttgtga aaaagctgtt
     7321 tagtttcgag tattaagaaa aattttgtgg aaatggcttt ccacattttt gcataaattt
     7381 ggcttaatac cgagaaactg gcttttgaac accgtttaag gaagaggttg tgaaatcagt
     7441 gccttgacct gagtattaga gaaacaaaac ggaaaaatag cttctcatat tttaccgttt
     7501 ttttagctaa tatcgagggt cagctgattg aacaccgttt atatcaagag gttgtgcgcc
     7561 tgccgttcag ctccgagtca caaggagtaa tttcaaaaaa cagtttttca tgtttgatga
     7621 ctattgtgac ttgtgaccga ggagttggcc ggagagcaac gtttatttaa aaaaaagaac
     7681 tgcgaactct cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag
     7741 agaagaaagc aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca
     7801 atcaaacata tacaaagaag gttattcaaa tggaaatctg atttgatgat gcttctgctg
     7861 ttgaaataaa gcagcatcta gtacctgaaa acaaactaat agtaacattc gaagatggag
     7921 taggtccgta ttttcagcat gcaatgatcc atatgtaagt ttaattcatc cttgaaatca
     7981 tcggaccaga taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa
     8041 agaacattta ctggaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag
     8101 tggtctgatt gacggaagct taggtgtgat cgatttctct ggaccaaaca acgaaggaat
     8161 caaggggaat ccggcacgtt aagaaaaaaa gtataaaaaa ctagacactt gatgagcgtt
     8221 cattcaattg attgaaggga ctaggctggt gtcatttttt atacatatat tttctctttc
     8281 gtcttgatag ggcttatggg ataatataag gaagaaaatc atcgttttat tcaaaaagga
     8341 gtttttgaca aatgaaaaaa cgtatattat ttatcggtga cagcattaca gacgcaggaa
     8401 gagaccgagc agaagcaact gatctaggta aaggttatcc acttttgatt gccggaaaac
     8461 taatctccca atatccaacg atgaaatttg aatgcatgaa ccgtgggata ggaggagaca
     8521 aaattgcaga tctgcaagta cgctggcaag aagattgttt agctttacaa cctgactatg
     8581 tatcgatttt gatcggaatc aatgatacct ggcataatac tagcgatcag gctatattcg
     8641 gaactgagaa atctgcgcag caatttgaat ccgtttatcg aaaattacta aatgaacttc
     8701 aaacagcgga aatctgcaac atcatcttga tggagccgtt tgtacttcct ataaccgacg
     8761 accgtcatgg ctggcgtaaa gacttagacc ctaaaatcca aattgttcgt aaacttgcta
     8821 aggaatatgg cgcattgctg gttcctttag atggcatatt caatgcttta gggattgctt
     8881 atggtttttc gaaatataca agagaagatg gggtccaccc aacgattggc ggacatgaga
     8941 tcattgcaca aagctggata gatgcaattg ccgcagtcga tttttttagt ggagataaaa
     9001 atgagcgaaa gaaaagtgag aaatgatgga aatacggtgg gcagtaaaag aagatttacc
     9061 tcaaatcaat cagctgatta ctgatcatcc agaatcattc gtaaaagcaa aaacggcagc
     9121 tgacctaaat gataagaata aactattgct cgttgctgta acaaaagaaa aacggattat
     9181 cggatttgcg caagcggacg tgcaacagtc agaagaacgg gtggatcagc gaccatcagg
     9241 aaaactaatc caacacacat tagtaacaaa agaaaatacg gaagaaatcc aaaaaaaatt
     9301 tgcagaatac ttgatgctgg aagctaaaaa aagaagaata aaatcaattt ttgcccagtt
     9361 ttaatcaaaa acagtaatag aaggagaaat acaatgagtt gtttaggaaa tgtcatctgg
     9421 tttatattcg gcggactaat cggtggtctt agctggttag cagcggggat actttggtgt
     9481 atcacgatca taggtatccc aatcggtatg caatgtttta agatcgcctc cctttctttt
     9541 gcgccttttg gcaaagaagt aatttatcac cagtctggca tgtcattgat cgttaatatc
     9601 atctggctga tcgtttcagg cctaccaatt gctctcgcac atgtcgtcag cggcatcctt
     9661 ctatgtatca cgatcatagg gatacctttt gccaagcaat ctttcaaact tgctcgattg
     9721 gcgttgatgc cattcggtgc agaaattgta taaaaagaga ataataaagg ttagaaaacg
     9781 catagagtca ggtgggatac atgtatttca cctgactcta tgtgttttcc tgtataagtt
     9841 ctcccatgtg acttttacgt cttttattta tatgggatta ctgtttatgg aaagttgaaa
     9901 taattttcat taaagaactc tttctatatt tttgaaagcg tagtggagct tcgaatatct
     9961 gggaaggaca ttttgcaggg attgaaaaaa gatattgtcc tatcgaaaaa ttaatgaatt
    10021 tagacaaatt gccagcaaca ggcttcaaac ttagttgcta tcctgtcaag atcaagaagg
    10081 caagtgcggg ttggataaga gcaggggcaa tgatcgaaga gaaaaagaaa gaataagcca
    10141 aaacatctgt atggaagaa
//
LOCUS       DAZKBH010000093        10074 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000093 DAZKBH010000000
VERSION     DAZKBH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.094"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..382
                     /locus_tag="ACG8OO_002221"
     CDS             152..382
                     /locus_tag="ACG8OO_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFP9046079.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            479..1780
                     /gene="murA"
                     /locus_tag="ACG8OO_002222"
     CDS             479..1780
                     /gene="murA"
                     /locus_tag="ACG8OO_002222"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9046080.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1793..1969
                     /locus_tag="ACG8OO_002223"
     CDS             1793..1969
                     /locus_tag="ACG8OO_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9046081.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2104..2400
                     /gene="yidD"
                     /locus_tag="ACG8OO_002224"
     CDS             2104..2400
                     /gene="yidD"
                     /locus_tag="ACG8OO_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9046082.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2464..2931
                     /locus_tag="ACG8OO_002225"
     CDS             2464..2931
                     /locus_tag="ACG8OO_002225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9046083.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3016..3900
                     /locus_tag="ACG8OO_002226"
     CDS             3016..3900
                     /locus_tag="ACG8OO_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9046084.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4014..4250
                     /locus_tag="ACG8OO_002227"
     CDS             4014..4250
                     /locus_tag="ACG8OO_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFP9046085.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4338..4418
                     /locus_tag="ACG8OO_002228"
     tRNA            4338..4418
                     /locus_tag="ACG8OO_002228"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4372..4374,aa:Tyr,seq:gta)
     gene            4425..4496
                     /locus_tag="ACG8OO_002229"
     tRNA            4425..4496
                     /locus_tag="ACG8OO_002229"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4457..4459,aa:Gln,seq:ttg)
     gene            4621..4791
                     /locus_tag="ACG8OO_002230"
     CDS             4621..4791
                     /locus_tag="ACG8OO_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046086.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5011..5718
                     /locus_tag="ACG8OO_002231"
     CDS             5011..5718
                     /locus_tag="ACG8OO_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9046087.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5778..6422)
                     /locus_tag="ACG8OO_002232"
     CDS             complement(5778..6422)
                     /locus_tag="ACG8OO_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFP9046088.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6549..6836
                     /locus_tag="ACG8OO_002233"
     CDS             6549..6836
                     /locus_tag="ACG8OO_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046089.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6944..7264
                     /locus_tag="ACG8OO_002234"
     CDS             6944..7264
                     /locus_tag="ACG8OO_002234"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9046090.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7345..8658
                     /locus_tag="ACG8OO_002235"
     CDS             7345..8658
                     /locus_tag="ACG8OO_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9046091.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8655..9374
                     /locus_tag="ACG8OO_002236"
     CDS             8655..9374
                     /locus_tag="ACG8OO_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFP9046092.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9502..10053
                     /gene="raiA"
                     /locus_tag="ACG8OO_002237"
     CDS             9502..10053
                     /gene="raiA"
                     /locus_tag="ACG8OO_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9046093.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3358 a         1666 c         2150 g         2900 t
ORIGIN      
        1 gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg gattcctata
       61 tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt ttattagtta
      121 ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg atgcggtgct
      181 tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac gttcgttacg
      241 cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa tcgtactttt
      301 ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg ctttatgccg
      361 aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag caaggatgtt
      421 ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag ggaacaacat
      481 ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa ttgaaggagc
      541 taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag gtattacgac
      601 attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga ttcgtcactt
      661 aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg cttcaagaca
      721 attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat
      781 gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat
      841 tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat
      901 tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt
      961 agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg
     1021 aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt
     1081 gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt
     1141 ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt
     1201 tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca
     1261 taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg
     1321 cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca
     1381 tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg
     1441 tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg
     1501 ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga
     1561 attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc
     1621 tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata
     1681 ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg tagacatgga
     1741 tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa
     1801 ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct
     1861 tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa
     1921 agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat
     1981 cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa aagaaaacag
     2041 aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa
     2101 ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca
     2161 tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa
     2221 acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct
     2281 tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg aaataagaaa
     2341 gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac tgagcgataa
     2401 agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata tgggggacaa
     2461 aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat
     2521 cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa
     2581 aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc
     2641 aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca
     2701 gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat acgaaccggc
     2761 agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga gattggtcga
     2821 gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta
     2881 agggatcaag taattttagc aattcactta cctaaaaata gctttaacta ataaactttc
     2941 aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa
     3001 aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat
     3061 ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg
     3121 gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc
     3181 gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga
     3241 atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt
     3301 caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa
     3361 ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg
     3421 gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg agaatttggg
     3481 ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt acagatggct
     3541 tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa agatgtcttt
     3601 gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat
     3661 cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc
     3721 ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg
     3781 acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga atacagaaac
     3841 gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg ggagaattaa
     3901 gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata ggagattttt
     3961 acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt
     4021 caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta
     4081 agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct
     4141 atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc
     4201 ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca
     4261 aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag
     4321 gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg
     4381 ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc
     4441 ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata
     4501 ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct accttgttcc
     4561 ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg agggatccac
     4621 atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca
     4681 gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa
     4741 tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa
     4801 aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa
     4861 catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg tctaaatcct
     4921 ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata
     4981 atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag
     5041 attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt
     5101 ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca
     5161 attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg tacatatgtt
     5221 gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa
     5281 tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca
     5341 agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt
     5401 cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca aaagctgatc
     5461 gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc
     5521 gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt
     5581 gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca gatttcttat
     5641 tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag
     5701 ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa ataaaagaga
     5761 atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata agaaacagga
     5821 ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt aaagtggtca
     5881 gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg gatttctttt
     5941 ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg atacgtgatc
     6001 atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga caatgcttga
     6061 gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa
     6121 tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac ttccaacatc
     6181 ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat ttcatttttt
     6241 tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt ttttatttct
     6301 tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca gataaagcgt
     6361 gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata gcgctcgatc
     6421 atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc taggttgttt
     6481 caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa aggtaggtgg
     6541 atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca
     6601 atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta
     6661 acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc
     6721 ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg
     6781 acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa
     6841 accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt aacagacgcg
     6901 aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta aaaaaacaat
     6961 catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc
     7021 tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg acgcagataa
     7081 caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc gctttatgaa
     7141 agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca tcaatatggc
     7201 tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc tcataggtga
     7261 ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc
     7321 gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga
     7381 gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca
     7441 gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac gtccgtcatt
     7501 gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt agggcgatgc
     7561 gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc
     7621 tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag aatactttgc
     7681 cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg
     7741 ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg
     7801 cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa
     7861 atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt
     7921 acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc
     7981 gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac agcgattaaa
     8041 ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact aaaggaaatc
     8101 aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt acttcctgaa
     8161 cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa gaagataaaa
     8221 tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt attttgtcca
     8281 agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc gagatatgag
     8341 atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa aatgcgagaa
     8401 gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac atttgaaaga
     8461 atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc tttggtacaa
     8521 atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata ctttttctat
     8581 gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat gaatgaagaa
     8641 gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa aatttaacat
     8701 tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca gtttgtcgcg
     8761 cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca atccatttca
     8821 atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt gactgtcaag
     8881 aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt gtgtacgatg
     8941 aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga ctagcaggca
     9001 cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc tattgtccaa
     9061 tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc gagatgttga
     9121 aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg ctggctcaag
     9181 caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt gcagtcacct
     9241 tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca acaggacaga
     9301 ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc cgaaccattt
     9361 ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca aataacctag
     9421 agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc ttgattgtgg
     9481 tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa tatcgaagta
     9541 actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg ctatttcagt
     9601 gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa
     9661 gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac ttctccagat
     9721 ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg caaattcaaa
     9781 acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc tactgcttta
     9841 ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg tacgaaacgc
     9901 ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat gctgggacat
     9961 aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa
    10021 gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata acaa
//
LOCUS       DAZKBH010000094        10073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000094 DAZKBH010000000
VERSION     DAZKBH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.095"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..347)
                     /locus_tag="ACG8OO_002238"
     CDS             complement(33..347)
                     /locus_tag="ACG8OO_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046094.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            633..872
                     /locus_tag="ACG8OO_002239"
     CDS             633..872
                     /locus_tag="ACG8OO_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046095.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1234..1494)
                     /locus_tag="ACG8OO_002240"
     CDS             complement(1234..1494)
                     /locus_tag="ACG8OO_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046096.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1679..2176)
                     /locus_tag="ACG8OO_002241"
     CDS             complement(1679..2176)
                     /locus_tag="ACG8OO_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFP9046097.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2306..3016)
                     /gene="budA"
                     /locus_tag="ACG8OO_002242"
     CDS             complement(2306..3016)
                     /gene="budA"
                     /locus_tag="ACG8OO_002242"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFP9046098.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3029..4693)
                     /gene="alsS"
                     /locus_tag="ACG8OO_002243"
     CDS             complement(3029..4693)
                     /gene="alsS"
                     /locus_tag="ACG8OO_002243"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFP9046099.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4901..5563)
                     /locus_tag="ACG8OO_002244"
     CDS             complement(4901..5563)
                     /locus_tag="ACG8OO_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9046100.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5573..6388)
                     /locus_tag="ACG8OO_002245"
     CDS             complement(5573..6388)
                     /locus_tag="ACG8OO_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9046101.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6649..7092)
                     /locus_tag="ACG8OO_002246"
     CDS             complement(6649..7092)
                     /locus_tag="ACG8OO_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFP9046102.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(7226..7564)
                     /locus_tag="ACG8OO_002247"
                     /pseudo
     CDS             complement(7226..7564)
                     /locus_tag="ACG8OO_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(7552..7929)
                     /locus_tag="ACG8OO_002248"
     CDS             complement(7552..7929)
                     /locus_tag="ACG8OO_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9046103.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            8153..9346
                     /locus_tag="ACG8OO_002249"
     CDS             8153..9346
                     /locus_tag="ACG8OO_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9046104.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9512..9934)
                     /locus_tag="ACG8OO_002250"
     CDS             complement(9512..9934)
                     /locus_tag="ACG8OO_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9046105.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         3044 a         1983 c         1641 g         3405 t
ORIGIN      
        1 taataattcc tgtactaaaa ggcataattg ctttaattat tttttgactt ctcttcaaac
       61 acaagaagta gttgtgtaat aattgcagaa ataaggattc caataataga tataaaattt
      121 gacatggaaa cgtctataac attaaaagaa taaagtgttg aagaaagtaa actgattgaa
      181 ataacccctc cgtaaaaaat agtctgtgaa tatacccact ttttttcaga agacgtactt
      241 cgtttggttc taaaacctat ccctgcattt ctactcttat caaaaaacca aatatttaga
      301 agtaataata ttgctattat taagataaaa ttactgtaaa aatccaaaag ctatcctcct
      361 agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct acgttttcac
      421 ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca gatggtaacc
      481 aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact aagactaata
      541 gatactataa aagacttgat agttttgcat tgattttgcc taatactgag cataggtgta
      601 tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt atcagtttat
      661 caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata ccttcagata
      721 cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat tctccggtag
      781 aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa ctgtcagcac
      841 gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag caaataatga
      901 tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt acgataacaa
      961 gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg gatcggatga
     1021 tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca gtataggttt
     1081 ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg aaccttttga
     1141 ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca aaagcttggt
     1201 cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat gttcatattt
     1261 gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag tatattttgc
     1321 cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat attcgtctac
     1381 gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt tttgtttcaa
     1441 gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat tcatatttca
     1501 tccatcctta atgttttttc attattcaaa atgataatac cattaaaata tgattgtgaa
     1561 aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag aaatgaccac
     1621 tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat agacgatttt
     1681 agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga tctacatatt
     1741 tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa tcgatatcta
     1801 aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat tgtgcggctg
     1861 aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat gtttcttgtt
     1921 tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa gaaactcgcc
     1981 cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct gcaaagacag
     2041 tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct aaccagttat
     2101 cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca tcttttttct
     2161 gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag attatagctc
     2221 agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca ggttaatcac
     2281 cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct gctaaatcag
     2341 cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc tgtacaaggg
     2401 acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt acatgtccgc
     2461 caaatgtatg tgtatcatcg ataaaatgca ggtgaaatcc cgctgcagca actccttgga
     2521 ataattcagg cgtaaagaac ccgactaatg ttccttggat attgctttgt tggaattctg
     2581 gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc ggcatgatac
     2641 ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg ttttccccgg
     2701 cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt gagacagaaa
     2761 aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca cttccatcta
     2821 attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa aagatgactt
     2881 caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat atatgagcaa
     2941 tcgtttctgt tccatccatc aagccggcca tcaaagctcc taatgtcccg tgctggtata
     3001 agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag attgtttctc
     3061 ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt ccttctgtcg
     3121 caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg attcctttag
     3181 cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca cctgacgccc
     3241 gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca ttccaaatca
     3301 aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca gagaataaaa
     3361 atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat gcagcagaaa
     3421 tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac aaatgacgag
     3481 gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca tctacggtaa
     3541 cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa ggatgcaaga
     3601 tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattttgct tgaagagatg
     3661 ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct gtcaataaat
     3721 caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa ggatcgattt
     3781 cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc cagtttcttg
     3841 cttcatattc gattggatca tagccaattg caataactag atcacttcgt ttcaaaagca
     3901 tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct tctaattcac
     3961 gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc ttttcaacta
     4021 attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact aatagtaccg
     4081 gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca gaagcagaac
     4141 ctaatttagg atcgctcaat ggcttgatga cattaccttg gacaggtgca tccactacat
     4201 cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata cgataagcat
     4261 tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat tttgtgattg
     4321 gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga tcgcttcgtt
     4381 ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt accaaacctg
     4441 tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc tctcctgtaa
     4501 tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc acgattaatt
     4561 caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct ggtataccga
     4621 atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa ccttgtttca
     4681 ttttttcagc cattgaacat cgtccttttt atgctttatt tttaattatt agttagaaat
     4741 aatcacaaat aagtgataca gtttctaaac ctgtttcata caaaagtata gcacgtttta
     4801 tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt ttacataaaa
     4861 aaaactgtca ttcttaattg aaagtttcaa tttataaagt ctacagatcc atagttctca
     4921 tcaatttacg attgttgaag taaatcaaac caaatacgat agaaggacct agtagaatac
     4981 accattgggg ctttacgaaa aagaagagaa acaaacttgt caatagtaaa aaatctagta
     5041 aactcacata ccattttttc aacgtatagt agaacgctat ccgtagaact tgatttggtt
     5101 tcgacgctgg atttctcacc tgaaaatacc agcagttggt gctaaaggaa actgataaac
     5161 aagcaagtaa aacaaacaac ggaatcaacc attttccata agaaaaacgt ataaaaaaga
     5221 aacaatcaaa aatcattatc acactaacta tccaagcaaa tagccaataa aaaaatccct
     5281 ttagccaaaa ttggcgaaac atctttatga acaaacgtac tggtgaaaaa ctattttctt
     5341 ttgctaattc ttctgtcata cctatcatca cgatgcaacc tggaccaata aaaagtaaag
     5401 aaaaaataaa ggaaaagaca ttccttccat ctatcgacaa aaggaacgca gctgctatat
     5461 acaagatatt gacacaccaa aaacaaaggc tacttgccaa tgattgatac aaccaataaa
     5521 agaattccat ataaacattt ttgtcaaaac gctgctcttt cataatcacc tcctattcct
     5581 tcattcctga tgaagcgatc ccttctacaa aatatttttg catcgctaaa aatataaaag
     5641 caacaggaag aacagaaaca actgaggcag ccatgattaa tgcgtactct gcatcaaata
     5701 gcccaataaa cattttcaac cctaactgga ttgttttatt ttccgtagaa gacaaataaa
     5761 tgaatggccc catataatcg ttccatgtat taacgaatgt gatgatcgtc aaactggcaa
     5821 tcgccggttt tgtaagaggt aaaatgatct tgcgatagat tccccactct gataagccat
     5881 cgatccgcgc actctcacat aaagaatctg ggatactact gtaatattgc ttcaacaaga
     5941 acacaccaaa agcattgaac gcttgtaata gcaccaacga ccacaacgta tctgttaaac
     6001 ccacttttct catcataatg aactgcggga tcatataaga ctgccaaggg acagcaatcg
     6061 tgccgatata tgctaaaaat aaaatatcgc gccctttaaa atttagttta gaaaacccgt
     6121 aagcagcaaa actagaagta aacaactgaa tcaacgtgat cacaacgctt aaaaaagctg
     6181 tattcttaaa aaatgtcact aaaggaatac gagaccaaat aaccagataa ttctctaaat
     6241 gccaagtttt agggatccat tgaatcggaa tcgtgaacac ttcattattt gctttgaatg
     6301 aagcagaaac catccataaa aaaggaatga tcgtcaataa agctaataca atcagcagaa
     6361 tcatacccaa ccattgaata aagggcatgt tttttgaacg aatttttgct ttttttcttt
     6421 catttcgaac acctccaaag ttataatctt catttctttg taatgattac aatccagaac
     6481 aacggttatt atagctgatt catttccgtt cttcttattt ttgtaagcgc ttatttaaca
     6541 agttatgtaa atctctttat ttttttgatt taaaaatgaa aaaatacaaa agcaagactg
     6601 tcatacgtct cgcttttgta ttgaaatttc ttcgattaga gatacttatt actgttcaaa
     6661 agcttctttg aaacggtgaa tcaattcatt gactacaggt gataaaatcc gattcttttt
     6721 ccagactagc atgcagttcg tttcaatcat tgggtcaagc ggaacgaatg tcactcgctt
     6781 tgaatgatga cttacagcac cttctatgac taaagcagca cctacctgat tctcaattag
     6841 cgcaaataca ttgaaaatca aattgtacgt tcctgtgatc agcaactgct ccttttttat
     6901 gcctaaccag tcagcaaaca ttcgctgtat ttctggacgc tttgagatca aaagaggtac
     6961 attcgcaaag tcttcttttg ttatcacgtc tttttttgct aaaacagaat tttctaaaat
     7021 gagcaatccc catttttcct ttcttggtaa caaaatcgtt tcgtatttct ctgtatctac
     7081 tggatctagc aaaatcgcta aatctaacag gcttttttct agtcactccg tgatatcatc
     7141 acttgttccg ctgtggatat gaaattgaac gctgggataa tcacggatca acttttctag
     7201 taatagtgcc aatgtattcg aactatcagc ttccacacag ccgatcgtaa atcggccgct
     7261 gaataattgt ttttttctgt tctcgaattc ttgagtggtt tgattcgtta aatctaaaat
     7321 ttcttctgct cggctttttt agaaaataac ctgcttctgt caagatcatt ttgcggtttt
     7381 cgcgtacaaa cagttctgtt cctagttact cttctaactc cttcagttgt cggcttaagg
     7441 ttggttgtgt aagatgtaga acttctgcgg cttgagagat tgttccttct tctgcaattg
     7501 tccaaaaata ttttagtaag cgtaactcca tagcgatgct ccttgatttt tttacttgat
     7561 acattccatt ttatttctag ataacacacg atcatatttc ttgatttttc cttgtaaacg
     7621 ttccaaacat tcttgcattt cagcaatttt tttcgtcaaa agttcacttt cttcaatcaa
     7681 aagcttacgc cgctcaaata cagttgcttc tccttcgcta gataggcgaa tatattcaat
     7741 caaagactcg atcgataatc cggcatttcg catacagatc gtaaagtcga cccattttag
     7801 gtcttcttct gtataatcac ggattccgcc ttttgtccga ttaactggtg gaatcaaccc
     7861 gattcgttca taatagcgca atgtatcggc cgaaatgccc ttttcttcac tgacttgttt
     7921 gatattcatc tgatcactct ttctcttaat ttcttgccac tttacttatg tattttattt
     7981 attctacaac tttaagtgta ctccaagtca atactcattt ctattaaaac taagctggat
     8041 tatctatatc ggttgtttgt gtattatttc aaataaaaaa gtcttgtttt tttattcttt
     8101 tttggatata cttttaacaa tttagggaaa ttttaggaaa aggggtataa aaatgacaac
     8161 gtatcagcga aaggtattag tatcgacatc ggcgggaatt gctttagaga atatggatat
     8221 tatgttttta gctttttcat tatcttcaat gattacttct tttcatatta gtggtacgca
     8281 agcgggtttc atttcaacga tcactaattt ggggatgctc attggaggta ttttttttgg
     8341 catcatagct gataaatatg gacgtgtgaa agtattttct caaacggtcc ttctgttttc
     8401 gattgcttct ttattgatgt attttgcttc aaacatctac ttggtttatc tctttcgctt
     8461 tatcgcaggt gtaggagcgg gaggagaata tggggcgtgt atgtccttga tttccgaaag
     8521 tttttcaaaa aaacaaattg gacgagcttc ttcggttgct ggtattggtg cgcaggtcgg
     8581 tgcggctttg gctgcgattt tagctgctgt gatcattcca acgtttggtt ggaagatgtt
     8641 gtatgtcgta ggtgtactgc cggtgttgat ggtcttgttc attcgtcgcg gcttgactga
     8701 accaaaggaa ttcaaatctg tacaatctgc cgaaaagaaa accaaactta gcagactgtt
     8761 tgaatcaaaa catcttgctt ggcaaacatg tggccttagt atcatggtaa ctgtccaaat
     8821 tgccggctat tttgggttaa tgaattggct accttcaatt atgcaaaatc gattagggct
     8881 ttcggtttct gggtcttcat tgtggatggt cagtactatt ttaggcatgt ctttggggat
     8941 gatgactttt ggcataatca tggataaatt ggggcctaaa ctagcctttt ctttgttttt
     9001 aatttgttca tccttatctg tctatctact tgtgttagcc catagccagt ggagtttggt
     9061 agcagccgct gtgatagtcg gctactttat caatggcatg tatggtggat atggcgcagt
     9121 gattagtagt ttgtatccaa cagaaatccg cgcgacggcg aataatttcg ttatgaacct
     9181 tggacgagct gttggtggtt tttcttctat cgtcatcggt ttcttgatgg atcattatag
     9241 tttaactgcc gtaatcttgt ttttgagcgg tatttaccta gtcagtctgc tggtcttgac
     9301 aactcttact ggtgttacag cattaaggga aagcttgcag cattgatagt tgagtctgta
     9361 aatgacaaga aagaggtgtg gaaaaagctt gcatctgcgg taataagacc agccaaatca
     9421 aaaacatgag acactgtttc cgtttggctg gtcgtattac ttgatgcagg gcagcttttt
     9481 caactgctaa tttccttcta tctggcggcg actattcttt gacgtccgtc atttcaaagt
     9541 actttactaa taacttagca taacgaaaaa ctgtggatac actcgtatat tgagcgtctg
     9601 cataatcttc caaatttttc tcaggatgtt gatacatgaa ttcaatcaaa tgataattat
     9661 atgattttct gtagaaatga agataaataa tgtagattgg gaagttctcc tgcttttcca
     9721 tagaaaagcg ccgattattc tcattcttca aaatgacctt atcaggatat tgttggaaca
     9781 attcttctaa atctgttaga taattcaaga tagacttact tgataccttc aattcgtttt
     9841 gtagttcttc tattgaacac caacgttctg tttcaagcag taaatgaatc attttaattt
     9901 tcttttgaga aacttttgtt aaaaaagtct gcatattttc tcctcctgta tctactttac
     9961 tatactacat acaagaaaaa aacggataca aatatcatat tgtcacaccg tcaatagtgc
    10021 ataaacctgt tattatttct ctttttttcc taagtattct attgatatga atc
//
LOCUS       DAZKBH010000095        10069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000095 DAZKBH010000000
VERSION     DAZKBH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.096"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..1416
                     /locus_tag="ACG8OO_002251"
     CDS             181..1416
                     /locus_tag="ACG8OO_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9046106.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2415..2675)
                     /locus_tag="ACG8OO_002252"
     CDS             complement(2415..2675)
                     /locus_tag="ACG8OO_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9046107.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2702..4003)
                     /locus_tag="ACG8OO_002253"
     CDS             complement(2702..4003)
                     /locus_tag="ACG8OO_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9046108.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4295..4753)
                     /locus_tag="ACG8OO_002254"
     CDS             complement(4295..4753)
                     /locus_tag="ACG8OO_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9046109.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4766..6097)
                     /locus_tag="ACG8OO_002255"
     CDS             complement(4766..6097)
                     /locus_tag="ACG8OO_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFP9046110.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6108..7550)
                     /locus_tag="ACG8OO_002256"
     CDS             complement(6108..7550)
                     /locus_tag="ACG8OO_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9046111.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(7537..8301)
                     /locus_tag="ACG8OO_002257"
     CDS             complement(7537..8301)
                     /locus_tag="ACG8OO_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFP9046112.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8405..9280)
                     /locus_tag="ACG8OO_002258"
     CDS             complement(8405..9280)
                     /locus_tag="ACG8OO_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9046113.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(9535..9619)
                     /locus_tag="ACG8OO_002259"
     tRNA            complement(9535..9619)
                     /locus_tag="ACG8OO_002259"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9583..9585),aa:Leu,seq:caa)
     gene            complement(9637..9708)
                     /locus_tag="ACG8OO_002260"
     tRNA            complement(9637..9708)
                     /locus_tag="ACG8OO_002260"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9674..9676),aa:Gln,seq:ttg)
     gene            complement(9718..9790)
                     /locus_tag="ACG8OO_002261"
     tRNA            complement(9718..9790)
                     /locus_tag="ACG8OO_002261"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9755..9757),aa:His,seq:gtg)
     gene            complement(9852..9936)
                     /locus_tag="ACG8OO_002262"
     tRNA            complement(9852..9936)
                     /locus_tag="ACG8OO_002262"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9900..9902),aa:Leu,seq:caa)
     gene            complement(9957..10027)
                     /locus_tag="ACG8OO_002263"
     tRNA            complement(9957..10027)
                     /locus_tag="ACG8OO_002263"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9993..9995),aa:Cys,seq:gca)
BASE COUNT         3247 a         2117 c         1566 g         3139 t
ORIGIN      
        1 tgttgcgtgt ttttttgaaa attggagttt caacttgatt acaaaattta aatttagaag
       61 ttgtgaaaac agcgagagac agtttaatat tcaaaatata cataggctac tcgaacgctg
      121 tgttttttag tatactagta agaaacgaag ggatagactc attccaataa ggaggatttc
      181 atgaagacaa ttctttttgc tcctgcagta ttcaatttag ctgagacaac acgaatgatt
      241 gaggttgcca aagctatgaa agaccgttat tactgtgtat tctttggctt ttctaaaact
      301 tttgctcctt tgatcacaga aagtggcttt gagtatcatt tactagcgcc gatccttacg
      361 aaaaaacaag aagaacaaat cataaaattt gaccagttgc gtacacttaa aaaccctttt
      421 accaaagaaa tggttgcctc tcgtatcact agtgaacaga gtctgattgc taagtttgag
      481 cctcgactga ttgtgacagg ttcgaatgtg acaatttttt tatcggcccg caataaaaag
      541 attcctttag tatatgttaa accctatgct ttgagtgaac ctttttttca atcagagcat
      601 gcgctgatgc cccacgagtt acgtttcttc ggccctttaa gcaatcccct ttggcactgg
      661 acaaaaaagc gtatagtaaa tacccgatgg atccccaatg ccttcaaaca cgctgcacaa
      721 aaagaaatgc ttcctttacc aaaaacaagt ttacaaatga tggatgctga tctaaatctc
      781 atcacaacgc ctaaactatt tttggaagaa gaacagctgc cggaaaatta tcgaatcgtt
      841 ggtccaattt ttgctaagct cagtacttcc ttgcctgaag aagtgctaac ttttatacag
      901 cgaaagcaac aagaaagaaa acgtattatc tatttcgcaa tgggtagttc aggcaatccg
      961 cgtatgttac gcaaaatttt gttcttctta caaaaacaac cggatctagc ggttattgca
     1021 cctattgact atctgcttga tgacattcaa tctgactcag aagatctttt aataacagaa
     1081 tatctgtcta gtcatctttt gcatgaattc attgattttt cttttatcca cggcggggaa
     1141 gggaccgtcc aaactgcctg tgaatccggc aaaccgttta tcggtgtggg gatgcaattt
     1201 gagcaaagct gtaatattga ctattgtgta aaatacggaa acgcaatcgc cctcacaaaa
     1261 ccagtgactg aaagaaaact gcagattgct ctgcgcgaag tttcttcacc caagattcgt
     1321 cagcaggcac gcacgctgca agccgttttt ccaataaatg gtccacaaca agcaattcaa
     1381 gaaatcgaaa ccttcctgag caaccaccca atatagtttc aagtagaaaa agctttttta
     1441 atagagactc atcagtaatt tcgctattcg agacccagac agtgaaaata caaaaaaagt
     1501 aaatagtgga ttgaaaagtt agagttactt aaattacggt gtctaaaaaa cacaaaatca
     1561 tccttaaaat aaaagatctg acgctatcca aatgagcata ggtagcgtct gccatttatt
     1621 cttagtcaaa ttctttgaca cctttctctg gttggatcat ctcaacgttt cttcaaaaga
     1681 aagaccataa taattaggca aatacatttt cttaaaatgt gtcatataga tctagaagca
     1741 tcgctttgta gagatttgtg ccgctagtcc cctcaacaat tactaaatca aatgtaggat
     1801 ttaccacttt atctttgata atcaacctcc ctcttctgtt gagacaatga tctcgcaatc
     1861 cgatgcatac tcctatataa actgccagca atccccacat attgagtaga tcatgtcaga
     1921 atgttctatg tcgtcagcaa cgatacacta aaagtcacgt tcaccatgac tgattgctat
     1981 tcccagcaca attatttcta tacacgtttc atgatttgaa taaacactca catcatacat
     2041 attaccattc atacaccata ctgcagcccc tactatatgg ttgtaagctc ccaaatctat
     2101 atttgacttg atcactttgg tagtagatct actaattcta aatccattgc tgataaaatt
     2161 tccatttttt cctccttctt tctgccaaaa cagttaaaac aataataatc tttatgtaaa
     2221 aaagggctgt gacaaaagcc ttgtcacagt cccatattcc gaataaacgg tggtcaaaag
     2281 cttgcatctg cggtaataag ttcaaccaaa cgaaaaataa gccaacattt cacaaaaatt
     2341 taagaagcaa ttttcgtaaa tttcttctta tttcttgaag ctaaccactt ctgttacaac
     2401 cacttttaga acatttaaag cttcgatgtg acaaaccatc tatgctgctc gatcataaaa
     2461 ttgatagcaa aaattccaca aagtacatat gtcccccatg aaaacaaaac attcatatgc
     2521 ggtacaatga tctttggaag aatcacgatc aaaaatgatg ccagtgccca aatccatcga
     2581 ttgatttgaa tatgatatgt agctagcata ttcaatataa ggtgactagc cgttaataaa
     2641 aagataaaaa caaaaaataa aatcagccaa tacattgttt cccaccttct aaaaaccaaa
     2701 cttagacagt ctggacttcg ttttctccac gactttgttc atctgacagc atctttttat
     2761 cataagcttt tacaaatgga taatagataa taaatgctat caatgtgttg atcatgatca
     2821 agataacaac acgccagtca tttcctgctg aaagataacc gccgattgga gctggtaatg
     2881 tccatgttgg caaaacagta aatctattga ccatttctaa tttgactgca aaataactga
     2941 taatagtcaa aactaaagga actaagttga acgggatgaa catgtaagga ttcaacatca
     3001 taggagctcc aaaaattatg ggctcattga tattgaagat accaggaata atagaaaaac
     3061 gaccaacctc tttcaagaat tttgatttac agaagaatat aaacaaaaca gataccacga
     3121 tcgttgctcc tgcaccacca attgttaccg tccattgata aaactgttca ggagcgatat
     3181 agggtaattg ggtggcagct gtaccatctg ccaatgcctt tgcattctca tccaacatga
     3241 tcagccacat tggacgaaca acagccccta caatactcat gccatggata ccaaatgacc
     3301 acaacaaatg gatcaaaaac attggtagta aaacaccaag caaattattc cctaaaatat
     3361 tggttaaagg ctcgaataac gataataagg cagcgtttac atcaaagccc gcgacgcttc
     3421 gaatcagcca agtaattgtt aaaatgactg cacctggaat caatgcctca aaactacggg
     3481 caacagcagg tggcacttgt tctggcattt tgatagtaat atttcgattt ttaaagaaac
     3541 gatatacttc cactgcaaaa atcatagata agatccccgc aaacatccca gatgctccca
     3601 aagaactcat aggtaagacc cagccaagag gtgcctcttc aggagttaaa acgtttacag
     3661 gaacttgtaa cataaagaac gttcctaaag ataatatacc acctgtcaca ctatctaatc
     3721 catatgattt agctagatcc gaacccataa caaaagttgc ataaatagcc atcaatccca
     3781 tcgtcatcct aaagggtaaa acaatcgttg ccgaataagg ggcaataaat tcattccatc
     3841 caggaaacgg taaattgata aagaccataa aaaatgtccc aatcaaaatc aatgacaatg
     3901 ttgaaacaac cccattacga attgctaata aatgacgctg tgtaccaatt tttgccattg
     3961 gtgggacaag tttctcttcg acccatttca tcagcttttc cactaaaacc actccttttc
     4021 aataaaatat gaaccattca ttgcatgaca acgcaaagaa tgttcataac taattatata
     4081 attaagaaat tttatttcaa tatattctct taaataatca aatttatttt taagaatcct
     4141 gaaaataagt tttctaaatc ataggagcaa caaaaaaatt cgataattaa caaaaatcaa
     4201 aatgattaaa tctagtgatt gaaacatatc atttcaaaaa aattttgtaa attcacccat
     4261 aaatatcacc ctatttaact tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa
     4321 tctttacttc ttggttttgt tttaataaat ttccttcaaa aatgcctgga gaaactacaa
     4381 tgatcaccgt atctttttgt gcttgctcca acttcttcaa atctataata gctaacagtg
     4441 tcccttcttt tacctcctgg ttactttcaa cccgtatctg aaacggactt ccttcgagtt
     4501 caactgtatc taaacccata tggatcagta tttttctgcc agattcactt tcgatcgtta
     4561 tggcatgtcg ggtaggaaat atattatcga ttattccttt aactggcgca tggatatatc
     4621 catcatgatt cgttatagcg tatccaactc ctatcatttt tgaagaaaag acctcgtctt
     4681 ttactgctgt taatggaata atttgaccac tagctacagc ttttagaatg tttcttgttt
     4741 taaagaaatg cactttttat cacctctact ttaatgctgg ccaatatttt ttgtttactt
     4801 tgatcatttc atctagtatt ttttttgcga ctctggcatt tggtacggtt tgattcaatg
     4861 taaatgcttg taaagctttt tgataagaac cttcaaagta agcatctact agtaacttct
     4921 ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc actgattggt tttcttagtg
     4981 taattggctc cacaccagtt gcaccaacat atgccggtat ttcaacaact gcatctgacc
     5041 gtagattcgg aatcgcccct tgattaggaa cgattagcat aaagcgacga cgatcatcat
     5101 tcaacaagga aatagctata tcgacgatat attgtccatg ctccccaaaa ttaaagttca
     5161 atacttcttc cacaataccg cggcgtattt tatcagccat ttctttggta tttttctctc
     5221 gtccatccat gaccatatta gcacgtgtat actctttgtc agtatgctca acgaccatat
     5281 caggatataa gtaatactct aagtaattat tcgggatatt acttggaaaa tttttcgtga
     5341 tgactgacat catttgaaag gttttttgcc aagttttatc tcctatatta aaatcggcga
     5401 cttgcatttc ttttgttatt agtttttcaa taatctctgg cataatttct cgttgcaatt
     5461 ctttatcata aatcgatgta taccagccaa agtggtttaa accataatac gtaacgatcc
     5521 aattcttacg atcataaccg taattcactg caatcgtttc ttcaattgaa atagtcatat
     5581 cacaggcatt aatcattttt acattcggat atttacgacg aacagcttca gaaacgatcg
     5641 actctggatt tgtataattc aaaatccatg ctttgggggc atagtcttgt atatgatcga
     5701 caatttccaa taaacctttg attgagcgca tcccataagc aaatcctccg agtccacacg
     5761 tttcttgtcc tactaatcca tgtttaagtg gaatcttttc gtccttttct cgcatctcca
     5821 atcctccaac acgaatttgt gagaaaacaa aatcgcatcc tgtaaaagca agctttggat
     5881 catccgttgc acgaatgcga atatttgaaa aaccttttct tttcagcata aatttaacaa
     5941 tcaagtacat atccatattt ctttcttcat caatatcata taaacgtatt tcagataatg
     6001 gcaaccgttt ttcattgttc aaaatggctt gaacgattcc tggtgtatac gtactcccgc
     6061 ctccagctat agtgataatc tgttttttca tcatcatctt ttcctttcta ttcttctaat
     6121 aatttattga attctgccat aacataagtc acttctaagc cgatgataat ttgataattt
     6181 ttttcagttg gtttaatgat cccgcttatc cctaattgct tgattcgctg ttcctcgacc
     6241 atggtgggat ccactacctc tacccgtagg cgtgtattac aactatagac atccaggata
     6301 ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca tatattcata atttttactt
     6361 gctaacccta actctgtttc ttctaaggtt acttcattgc caaactctgc cttagcttca
     6421 cgtcctaatg tctgaatgtt tctcttattg atcaaataat aaaaagtaac gtaatacaag
     6481 gcaaaaaaga aaattccgat tggaagaatc aaaatagcat tggtagcgat tcgaaaattg
     6541 atgagataat caacaataca tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa
     6601 cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca cgtacaacat aggagaaaga
     6661 aacatgaaac taaattcaac aggctcagtg atatttgcaa taaatgaagt tcctgcaccg
     6721 cttcccatga ttcctttgac catctcttta ttttctttat aagctgcttt atagatagct
     6781 agacatatag ctggtatacc aaacatcatt actacaaaaa aaactgataa aaacagtcca
     6841 gcctgaggat ctccgttgat aaagcgtgtc atctctcctc tgaaaacttc cttgctagga
     6901 gatgtatagc ttcctaaatc gtaataaata tacgtattca acacatggtg cagtccaaag
     6961 gggatcagca aacgattcaa aaacataaat atccccacac ctaatgctcc catatcaacg
     7021 agcagttttg caaaattatc aatcccaatt tggatgagcg gccagataaa gcccaaaaaa
     7081 acagctgcga ttgtttgtag aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa
     7141 taagaaaaaa ctactggtaa tttttgttct ttaaagcgat tatacgtcca tgccgctact
     7201 aaaccggaaa taattccacc aaaaatcccc atattcactt ctgggtccat aatttctaag
     7261 ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta
     7321 tttttgacaa aaccgattgc aactcctata gcaaataaga tatccaagtt tccaaaaacg
     7381 gcatttccag ctgcaccaat tactgggatg ttcagcatat cgtcagctga taaacgcatg
     7441 atcaatcccg ctaatggcat cgctgcaact ggaagtagca tcgctcttcc aagcttctgc
     7501 acatttgatt taacattcat ttttattcct cctctattaa atatcctcat atgttactaa
     7561 ctcaatcaaa ggatcatttt tcactttttg agcaaatgct gaatgaatta aaaattcgac
     7621 ttggtgggta cgaggataca caaaacttga accatttaac aattcctgat ccaaatacgc
     7681 tgcgtgacac atgacttcga tcgttttatc ttctctcaat atcatgagta aacttgccaa
     7741 atattcttcc acactcgtat tgatttgtcg ttttttttca tcagtttctc ctacgacatc
     7801 gaaacaaggc tcaaaataat cgacatgacg aacttcatct tttcgttcaa agttgctccg
     7861 aaccggcaaa tcatattttt ttgctaatgt aacaactacc tcctgatttt catccaaagt
     7921 atgagtatga tgatgactgt ctaaatgagt tggtatgatc cccgcatgat aaattttttg
     7981 aatctgagca ttccattcct gatacagttg atctttgtca atgttaaatg gatgctgata
     8041 ataagataag gatctgaact ctccgctttc gataagagta tcgacagttt tgaggagtgg
     8101 tttgccacac gtcaatgtta aatggactcc tacacctagc ctaggcattt cctttcttag
     8161 tttcacagca tgctcgaaac ccggcatatt tgccatcaat gtagttgatg tcaaaattcc
     8221 tcgctgataa gcatccataa tcccataatt cacaccatgg ctatagccaa aatcatccga
     8281 attaaaaatc acttttccca tttcattctc cttctttttt ctactaaaat aggcgcaacc
     8341 aaataatcat agtccattta ctatgtgtca tttggttgcg cctatagata gttacgccct
     8401 tattttaata gattcttgct agatgaattg ccaaatagat gatttcattt tcttgaatgg
     8461 caagttgaaa ttgctgttcg atgatatctt taatctttag tgctgtacaa tacgctttgc
     8521 tatgtttaaa tgtttcagta aaaaaaccaa tattttctac tggcgtttct tcctgataaa
     8581 tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg atgataagtc agtgattctt
     8641 tatcataaac gtttgaatag gatttttcta gatgggacaa aatcgtggtc gtcatttcta
     8701 ctatttttaa gctctttaaa ctattaggct ctcctagctc agcattaatg atatgcatag
     8761 tgatgaatcc tacttcattt gaccctaact cggtttgata tcgatgattg attcgctcga
     8821 ccacttctac tgctatatca tattcatttg gataaaggaa tctcatttct ggcaacataa
     8881 ttgctgtgac atctctatct tctctttttc gttgaatagc aaaataaata tgatctgtca
     8941 atgtaatata gatatttgaa tctaatggag aggagatcct agctgttatc agttcaatca
     9001 cttcttccgc aaaatccata aattcgattg gaatctgcga gactatctca ctgaatcgtt
     9061 tgatcttttc tttagaatct aatacaaata atttgtcagc atcctcaaca tcaaatggct
     9121 tactcacttt agcagaaaag ccaatccctt tccccatcgc aattttttct attccatcgt
     9181 catcgatcaa tacagcatta tgattcagct ttttcatcac atacaccgtt aattcacctc
     9241 ctctatatga taaaacaaaa caacttgcag actccttcat acatattttt gtctccaaac
     9301 aatttttaaa gacaaaaaac gacctaagaa agaacccttt cttaggtcgt gcctaattga
     9361 atagtagcac cctacataat ttacaaaaat agtttaacac ataataaaag cgttatcaat
     9421 atctttttta ctcgttttat gataatactt gtatcgaatt gatttggagg taattacata
     9481 aaaacaaaaa aaatatagca actctctata agaaagttgc tatattgata attataccgg
     9541 cggccggggt cgaaccggca cgtcctcaac ggacactgga ttttgagtcc agcgcgtctg
     9601 ccaattccgc cacgccggca aatttaaaaa taataactgg ggtagctgga ttcgaaccaa
     9661 cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaag aataaagggc
     9721 gaatgatggg aatcgaaccc acgaatgccg gagccacaat ccggtgcgtt aaccacttcg
     9781 ccacattcgc catcataaaa gatgttattt aatttctcaa taaaaaacta agatgaagac
     9841 atcttagtca ataccggcgg ccggggtcga accggcacgc cctcaacggg cactggattt
     9901 tgagtccagc gcgtctgcca attccgccac gccggcaagt tgatttatta gtactaaggc
     9961 ggtaaccgga tttgaaccgg tgatgaaggt tttgcagacc tctgccttac cacttggcta
    10021 taccgccatt atactattat caatctataa aataaagaat aaattatag
//
LOCUS       DAZKBH010000096        10036 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000096 DAZKBH010000000
VERSION     DAZKBH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.097"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..162)
                     /locus_tag="ACG8OO_002264"
     CDS             complement(25..162)
                     /locus_tag="ACG8OO_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFP9046114.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG8OO_002265"
     CDS             complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG8OO_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9046115.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ACG8OO_002266"
     CDS             complement(1599..1853)
                     /locus_tag="ACG8OO_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046116.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ACG8OO_002267"
     CDS             complement(1878..3020)
                     /locus_tag="ACG8OO_002267"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP9046117.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ACG8OO_002268"
     CDS             complement(3080..4432)
                     /locus_tag="ACG8OO_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9046118.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ACG8OO_002269"
     CDS             complement(4503..4847)
                     /locus_tag="ACG8OO_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFP9046119.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ACG8OO_002270"
     CDS             complement(4857..5231)
                     /locus_tag="ACG8OO_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9046120.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ACG8OO_002271"
     CDS             complement(5244..5558)
                     /locus_tag="ACG8OO_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9046121.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACG8OO_002272"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACG8OO_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="ACG8OO_002273"
     CDS             complement(8976..9638)
                     /locus_tag="ACG8OO_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046122.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
BASE COUNT         2705 a         2264 c         1714 g         3353 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgag
//
LOCUS       DAZKBH010000097         9965 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000097 DAZKBH010000000
VERSION     DAZKBH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.098"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(429..653)
                     /locus_tag="ACG8OO_002274"
     CDS             complement(429..653)
                     /locus_tag="ACG8OO_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046123.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            844..3621
                     /locus_tag="ACG8OO_002275"
     CDS             844..3621
                     /locus_tag="ACG8OO_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9046124.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3702..4121
                     /locus_tag="ACG8OO_002276"
     CDS             3702..4121
                     /locus_tag="ACG8OO_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9046125.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4133..4603
                     /locus_tag="ACG8OO_002277"
     CDS             4133..4603
                     /locus_tag="ACG8OO_002277"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9046126.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4620..5399
                     /locus_tag="ACG8OO_002278"
     CDS             4620..5399
                     /locus_tag="ACG8OO_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9046127.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5386..6207
                     /locus_tag="ACG8OO_002279"
     CDS             5386..6207
                     /locus_tag="ACG8OO_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9046128.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6224..7273
                     /locus_tag="ACG8OO_002280"
     CDS             6224..7273
                     /locus_tag="ACG8OO_002280"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9046129.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7286..8290
                     /locus_tag="ACG8OO_002281"
     CDS             7286..8290
                     /locus_tag="ACG8OO_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9046130.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8923..9942
                     /locus_tag="ACG8OO_002282"
     CDS             8923..9942
                     /locus_tag="ACG8OO_002282"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9046131.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3347 a         1646 c         2083 g         2889 t
ORIGIN      
        1 gaaatctaat tgattttcaa agaaggagta tgaaaatgag aaaaatattt attcgtttat
       61 tgtgggtact atcttagagg aataaataat agtggagaaa actatggttt ggcgcagatt
      121 ctatcaggtt tgatggaacg aagacaacct cccgacatca agtcaaaagc tacttgagat
      181 tttgatctga aaataaaaaa agaaaacaag gtaggaacat caatgtttct accttgtttt
      241 cgtaatcaat gggaaaaaac taagagtggt gtacgtaatc aataaaaagg atggtgacaa
      301 cagtacctct ttactctctg aaagcatcat aactcgtttt tcctcattat gccaataaat
      361 tgttgttata ccacatataa ggatttgtaa tagctaaaaa cagacaggaa agttgaaatc
      421 tgtgaatctt attttgtatg gaaggattta tagaacgcaa aaatcaagat aaagatgaat
      481 aaaaatcctg tttcggctat atattgcgta tctacgccaa taaaacgagt ttgttctgtc
      541 atagccatat aaagcaaagg caaaaataca actacagcga gataagatcc gatgatttct
      601 cgaaaagttt tccaggaagt atgtttcctg tttttatatt tatccgattt cattgaatag
      661 tcatagcctc cttattcttt catatgttga aattccagta taaaaactgg aaggaactgt
      721 acgtttttta gaataaaaat tataagggat aagtaggctg ctgtctataa taagatatat
      781 ttttcaattc tttattaaag attttattga caaaatgaat ccgttttcta tgatggagga
      841 ggggtgaata agttgttgaa gaaggaaatt ttatgttact tagagaacca gacagccttt
      901 ttcgatttag aacatatgaa cgaggtcttt acagcacaaa ggttagctga tcattttggg
      961 gtaaaaagaa acacagtcag tcattatttg aaccagttaa atgatgagca actactagtc
     1021 aaaatcaatt ctcgaccagt gatttatttc cacaaagaag cttttgaaaa acaatttttt
     1081 aagttacgca cgaattttta tttttctatc gatgatctca aagaggaaca gccttttttc
     1141 gcgcaaccaa aagacttatt ctcttttatg atcggatacg acgccagttt gaaagaaagt
     1201 atcgaacaaa tcaaaacagc tctttactat ccagatggcg gattaccact tttgattaca
     1261 ggcgaaagtg gcacaggtaa aagttattta gttaatttgg tctatcagta ttgtctgctt
     1321 catgatctgc tagaagattc tgcaccattc atcactttta attgtgcaca atatgcagat
     1381 aatcctgaac ttttgactag caatttgttt ggacatgtta agggagcgta cactggtgca
     1441 gaagaaaata aaaaaggggc ttttgaagca gcagatggtg ggatcttgtt tttagacgag
     1501 gtacatcgct taacaccaga aggtcaggaa aaactgttca cctatcttga tcaaggtgtg
     1561 atttatcgta tgggagaacc taatttgtca cgccggatca aaacacgtct cttttttgct
     1621 acgactgaag agattactag tcatttctta acaactttca ttcgaagaat acctatccga
     1681 atcgaactac ctgcacttaa tcagcgtagt cgtaatgaac ggctagaact gatttactct
     1741 ttttttctcg aagaacaaag aaaaatgggg agccctttgt cagtcagtgg accagctttg
     1801 tcacttttaa caagtcagaa attatccgga aacataggag agctgaaaaa tatcgtcaaa
     1861 gtttcagcag caaaagcata tgctgaacag cgtgaacagc ctgaaatcca tgtcacgatc
     1921 catcatttgc aaaaagaact acttgcgcag cctattacta aaaatacagc acagcaagga
     1981 atctatatta cgcaagacca gaccttagag caattaatgg agaagcaaca gcccgaacag
     2041 cagcgaatcg ttaaaagttt tgagcaaatc ctaattgatt tcaagaaaaa taactgtctg
     2101 ctatattctt gcgaaggaaa attaaagcaa gaagtgattc aattatttga ttttttgctg
     2161 tttgagacca gtagacaaga aaaacacgag ctgctggttt attggacaca atctatccga
     2221 gagacctttc gacaaatgga aactgcctat caaatcaaat tcgatggaaa cagtgtctat
     2281 gccttgagtt actatctcta tcagcgaagc actatcaaat ggttaccgga agaacatgat
     2341 attatccaac taatcaaaga attagaacaa caagtttctc agtcttatcc atctagttat
     2401 cactatgtcc aacgaattct ggaattaagt aagatcaaat tagatattga agacacaagt
     2461 atggatcgga ttttactgac gatctatcta aaaaaagcga aatggtcaaa agaaaaacga
     2521 ctgccaaaag cgatgattgc tgctcacggt tatgcaactg ctagcagtat ggcaaatgta
     2581 gtaaatcgtt tattacaaga ggatctattt gaatcatttg atatgccact agatgtcaca
     2641 ccacagcaga ttgcttcaga aatccttgat tattgcgaaa acagcgatgt ttcaaatgga
     2701 ttgatcatat tagtagacat gggatctctg aaagaaatcc atcagttttt taaaaaacag
     2761 ttatccgtac cgcttttgat tttgaataat gtgacgacac ctttagcaat cacagtaggg
     2821 gaatgcctgc aaaaaaatgt atcattagaa gagatagcag aagagactgt tcgacagatc
     2881 cagccagaat ggcgactgct ctatccagag gaaaacaaac ccaaagcact cattactact
     2941 tgcttcactg gaatcggtac ggcggtccac ttaagtgaac tgctagaaaa aagcttacct
     3001 acggcttgtc agttgaaaat catcccatat gaatatcagc aattaaaaga taagaaaaac
     3061 agtgacccat tattttccat atacgaagtc gtaggaatga ttggcaccac agatcctgat
     3121 atcaccaata tcgactatct ttcattagaa gacctcatct cgggggaaaa gatgtctgta
     3181 ctagctgaat ggttggacac tactatgaat gcttcagaga aggaaatatt caatcagcgc
     3241 atcattcgta atttttcatt ggaaaaagta ctggattccg tgactattct agatacagag
     3301 aaggtgatgg gagaaatcga tcattttatg agagaattag aggtgcagtt gaatcgaagc
     3361 atcagcaatc ccaaaaaatt agcgttgtat gtccatgtga gctgtctcat tgaacgattg
     3421 atccgacaaa tgccaatcga aacctatcaa ggattagaga aatggaagca gtgtcaaaaa
     3481 gaagggttat ctgctattaa atctgcgttt agtgtcattg aagagcatta tagtgtcatg
     3541 atccccaatt ctgagattgc ctatatctat gatattttga gtgaagatac agagtttctc
     3601 tcgatagaag aagaattttg aacataagca agttgttgcc actaattttg gcatgcaact
     3661 tgctttttta gtatgtgtaa gtgaaaagag gagatgaaaa agtggataga gaaattatct
     3721 tagcttccca tggaaaattt gcttcaggga tattagattc attagagctg atctatggga
     3781 aaaatcattc tattactgta ttcgattgct atactgatga gaattttgat ttagcggaaa
     3841 cagtaaggca gctttttatg aggtacaaag ataaagaaat cattgttctg acggatcttt
     3901 ttggcggaag tgtcaacaac gaattcttac aatatatcaa tcgggatcat gtctatctcg
     3961 ttgccggatt gaatttgcca ctattgattg agctagtatc tcggttggac gctgaagtag
     4021 agacaactgt tttgatccag caggtgctaa ctgattcaaa aaaaatgatc cagttttgta
     4081 acgagcggct caatcagaaa atagaagaag acgaatttta ggaggaagaa aaatgattgt
     4141 attgacaaga gtcgaccatc ggttattaca cggacaagta gctttttcat ggacacaaac
     4201 aataggcgca gactgtattt tgattgctaa tgatgatgta cctacgaatg agatacgaaa
     4261 gacaacgatc aaattagcca agccacaagg agtcaaacta gtgatcaaat caattgatga
     4321 ctcgattgcc gcgttgaaaa gcggggtgac agataaatat aagttattca tcgtagtgga
     4381 atcgatcgag gatgcatata agctggcaga agcgtatcca gaaatcaaaa ccatcaactt
     4441 aggagggatc aaggcaaaac ctggtactcg cagcatcgga aaagcggtaa atattttacc
     4501 agaagaggag gaacgcttga aacgattaat tgacaaacaa atagaagtag aaatccgtca
     4561 agtaccagca gataaaaaag tggatgtaag agaagttttg taagaaagga agaacctcaa
     4621 tgattgtaca agcagtctta ttaggaattg ttgcatttgt cgcacaatca gaatatgcac
     4681 tagggacatc gttgctttct cgtccgattg tgacggggct gtttacagga atcgtgttag
     4741 gtgatgtaaa aactgggatc attatgggcg caactttaga gttagcattt atcggatcgt
     4801 tctcagtagg tgcttcgatt ccacctgatg ttgtgaccgg tgggatttta gggacggctt
     4861 ttgctattac agctggagcc ggtacagaaa ccgcgctttt actaggtttg ccgattgcta
     4921 cgttgacatt gatattgaaa aatatttact tagggctttt gattccaatt atgaaccata
     4981 aagcagacaa ttatgcagaa gaaggaaatt ataaaggagt cgagcatatg cacttgcttg
     5041 ccggtatcgg tctttccttg atgcttggtg ttgtggtaat ggtttcttat atggtgggaa
     5101 gcaatacgat cggaaatcta ttaaatatga ttcctgactt cgttcaaaag gggttgtcag
     5161 tagctacagg gttgattcca gctcttggtt ttgcgatgct tgctcgtttg ctgatcaaca
     5221 aacaagttgc accatatttt ttcttaggtt ttgctatagc ttcttactta gagattcctg
     5281 tgaccggagt agctattttt ggtgcgattt tagcagttgt ggtagtcaat attatgaatg
     5341 tccgtcaact accacttcaa acaactgcag gggaggtaga agaagatgaa gaattctgag
     5401 atcaaaaaca gcaaaataac gaaaaaagag ctaaatcacg tattttggcg ttcttttcaa
     5461 atggagtttt cttggaatta tgaacgtcag atgaatatgg cttatgttta tgcaatgatt
     5521 ccgattttga aaaagttata tcagtcgaaa gaagaaatgg cagcagcttt gaaacggcat
     5581 ctagagtttt ttaacacgac cccacatatc gttacactga ttttaggaat cacggctgca
     5641 atggaggaag aaaataagga tgatcctgaa tttgatgtga ctgcgatcga tagtatcaaa
     5701 acatctttga tgggacctct tgcagggtta ggagattctt tcttttgggg aactttgcga
     5761 ttgattgcca caggtgtggg tacctcttta gccatgcaag gaaatatttt aggccctatc
     5821 ttgttcattt tgattttcaa tattccgcac attctgtttc gctatctagc aacaggttgg
     5881 ggatatcgct taggaacggg ttttttgaaa aagatccagg caaacggtat gatggaaagt
     5941 ttgacattag gcgcgtctat tattggactg atggtcgttg gagcaatgac tgccacgatg
     6001 atcaccatca atattccttt aaaaatcggt tcaggagaaa atgctgtgac tgttcaaagt
     6061 atttttgatg atatcgttcc aaatatctta tgtcttggag catttggcat agtgttttat
     6121 ctattgaaga aagaagtgaa accattaacg atcttgatcg gactagcttt ctttggaatt
     6181 tttggttcgt ggatagggat tttttaaaaa aggagcagag aaaatgttta cgatgcaaga
     6241 ttatatctat gaagaaaagg aagtactaag tacgatcttg aaaaagaatg atttttcgac
     6301 aagagaacat atgaaaaaaa cagtgaattt actgatctta gcaacgggtt catcttataa
     6361 cgcttgttta gcagcgaagc cagcattgga atcttatggt gatcttactg tcgatattca
     6421 agaacctttt tattttttca attatggaaa actttctcct tcaatcgata ctgtgattgc
     6481 tgtgtcacaa agcgggaaaa gtgcttctac tgttgaagca gtaaaaatga tacaaaaaca
     6541 agggttacct gttgttgcta tcacaaatga cgtgcaaagt ccacttgctt tagaagctgc
     6601 acaaattatt gatttaggtg ccggggtaga atcagtaggc ttcgttacaa aaggctattc
     6661 tgcgactgtt ttacagttac tactcttagg gataggaatt ggaataagta aaaataaaat
     6721 cagcaaaaaa atagaacaag actacatgca acagttgcgc aaaataatca accatttacc
     6781 agcgattatt caaaaaacgg aagaattttt tgatggatat caaagtttat ttcgtttagc
     6841 acagcgtttc gtcaccattg gatatggtcc aaactgggga acagctaaag aagcagagac
     6901 gaaattgact gaaacaatcc gcgttccgtc acaaggattt gagttagaag cttatatgca
     6961 cggcccatac ctggaggcag atgcttcgca tttgctattc tttattgaag gggaaagtgt
     7021 aaataaggaa cgttcgcaaa agttgcaaag atatatgagt cggtacgttg gagagactct
     7081 caccattacc acaaaaaaag caagaaacga gaaaacactt ggattagcaa tagaatgtga
     7141 tgaatatctg tctgtattag cgcttgtcgt tccgtttcaa ctattcgctt ataaaaccgc
     7201 agtagctaaa ggtattgact tgaacaaaaa gatttttgaa gactttgata cagtcttgaa
     7261 aagtaaatta taagaggtgg aaatcatgct gaaattcaat gaagaagaac aaatcaaagc
     7321 aataaaaggc gcactagctt tacgtccgca agtagaagaa atcatcgata aactttatgc
     7381 ggaaaaattt gacgctgtct attatctagg aatcggtgga acgtatgctt cctcgatgca
     7441 agctgtcact tatatgaatg ggaaaagcaa cttgcctgtc tttgtacagc atgctgcaga
     7501 atattacact actggaaaca aaagattaac aaaagattct atcgttattt tgtcctctgt
     7561 taccggtaca actcaagaag tagtgaaagc agtagaacaa atcaagaaag taggagcgac
     7621 actgattgga tttatcgata aagcgaacag taagttgtct cagctatgcg attttgtggt
     7681 cacttatcca gctcctggaa cagaacaaat caaatttttt atggcagcag atcgattgat
     7741 gtatttgaat ggagaattcg aagcttattc agaatactat gaacagctcg aacgttattt
     7801 accaacaggg ttagtggaag cagagaaaaa agcagatgca tttggcttat catttgcaga
     7861 aaaacatcga cgtgatagta tgcattattt tattggagca gggaatcaat ggggagctgt
     7921 ttattcatat gccatgtgtt actgggaaga acaaagctgg cttccatcga aatctatcca
     7981 tgctgccgag tttcttcacg ggacgttaga gatcgtagaa gaaacaaccc cagtcacatt
     8041 atttttaggc gaagatgaac agagagtgtt atctgaacgt gtggctaaac ttttgccaaa
     8101 aatttgcagc aattatacat tgatagacac aaaagactat ccagtagaag gtatcagtga
     8161 aaaatatcgc ggacgtgttt tgtctttctt attgatgcat gtagtgacac aacgcatcga
     8221 tgctcatgtg gagcagttga actgccatcc attagagata cgccgctact atcgtcagtt
     8281 tgattattag gatagactgg ataaaaagaa tataaaaaat aaagagagca gcgataactg
     8341 ctccctttat ttttttaaaa tattggcatc aattacgtta taaaacacat tttttgcatt
     8401 atctacagtt caacttataa tctgtttaat gggacatgtg ggtgccaacc attttttgtt
     8461 atgcaatagt actatttagt tgttgcatgg atccgtagta aaagtccaag tacaacgagt
     8521 tgcgccagtt tacatagcgt tttgtatcca gaatgttgac taaaacaaaa gttcctatgt
     8581 caaccgtcaa cagagacaat ctgtccgttg caggacctga cagatttaga aatgttcaat
     8641 ctgtctctta tgtattttaa atcaatactc actaggagaa gtttcctaac ctcctaatag
     8701 taatgctggt agtaacattg tcaaaactgc ttcctcttag gaacaagcaa agcataacga
     8761 gtgtaaaaat aaaatgtaga ctattgtaac atatcattga ttatttcaag taaaaacgat
     8821 aaaagaagta aacaaacact taaaaacaat ggaaattagt cccaacttta agaaatgtat
     8881 actataatgg aaattatcaa ttatttattt ggaggaatat ccatgaaaca aaaaatcgca
     8941 gttcttggtc ctggatcttg gggcactgct ttagcacaaa cattggcaga aaacgggcat
     9001 gatgtccgta tttggggaaa tgtacctgaa cagattgatg aaatcaataa gtatcataca
     9061 aatcagcatt ttttacctga tttgacgatc ccagaatcga ttatcggata taaagaattg
     9121 gcagaagcag tggataacgc agatgctatt ttatttgttg ttccaacaaa agcgattcgt
     9181 tcagtcgcac aggaacttat tggtaaaatg aatactaagc caatcattat acatgcaagt
     9241 aaaggactag aacaagatac gcataaacga atctcagaag ttttggctga agaaattcca
     9301 gaagaaaaaa gacaggcaat tgttgtatta tctggtccta gccatgctga ggaagtggca
     9361 gtacatgata tcacaacaat tacagctgca agtattgatc aaaaagcagc agcctatgta
     9421 caaaaactgt tcatgaatga ttatttcagg atttatacaa ataatgatgt gattggcgta
     9481 gaaactggtg cagcactgaa aaatatcatt gcaattggtg ccggcgctat ccatggatta
     9541 ggattcggag atgatgcaaa agcagcaatc atgactcgag gattggcaga aatcagccgt
     9601 cttggtgtcg cgatgggtgc caatcctctg acatttatcg gtttgagtgg tgtgggggac
     9661 ttgatcgtta cttgtacaag tgttcattct cgcaattggc gtgccggtaa cttgttagga
     9721 cagggacata agctggaaga agttctagaa aacatgggaa tggtcgttga gggtgtggca
     9781 acgacaaaag cagcagtaga gctagctcaa cagctaggtg ttgagatgcc aattactcaa
     9841 acaatctata atgttttgta taatggagaa gatatcaaaa aagcagcaaa agaaatcatg
     9901 ttacgtgacg gaaaaatgga gaatgaattt tctcttcgtt aaatagatga tcaatagtta
     9961 atcgt
//
LOCUS       DAZKBH010000098         9963 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000098 DAZKBH010000000
VERSION     DAZKBH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.099"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..1366
                     /locus_tag="ACG8OO_002283"
     CDS             26..1366
                     /locus_tag="ACG8OO_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9046132.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(1436..3817)
                     /locus_tag="ACG8OO_002284"
     CDS             complement(1436..3817)
                     /locus_tag="ACG8OO_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9046133.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3807..4454)
                     /gene="recU"
                     /locus_tag="ACG8OO_002285"
     CDS             complement(3807..4454)
                     /gene="recU"
                     /locus_tag="ACG8OO_002285"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFP9046134.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            4518..5057
                     /locus_tag="ACG8OO_002286"
     CDS             4518..5057
                     /locus_tag="ACG8OO_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFP9046135.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            5147..5566
                     /gene="gpsB"
                     /locus_tag="ACG8OO_002287"
     CDS             5147..5566
                     /gene="gpsB"
                     /locus_tag="ACG8OO_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFP9046136.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            5588..5955
                     /gene="rnpB"
                     /locus_tag="ACG8OO_002288"
     ncRNA           5588..5955
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8OO_002288"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            6164..7330
                     /locus_tag="ACG8OO_002289"
     CDS             6164..7330
                     /locus_tag="ACG8OO_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFP9046137.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            7376..8872
                     /locus_tag="ACG8OO_002290"
     CDS             7376..8872
                     /locus_tag="ACG8OO_002290"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFP9046138.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8953..9636
                     /locus_tag="ACG8OO_002291"
     CDS             8953..9636
                     /locus_tag="ACG8OO_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9046139.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         3125 a         1876 c         2101 g         2861 t
ORIGIN      
        1 aaaccattca aaggagtgtt tcaagatgtc ctattcagag tttaccaaag agttactaga
       61 tattctagac ctaaatctta cctttcatga agatgccttt cgcaaagagc gcattaatga
      121 cgaaacatgt tttgtttttg atggcacatt gacctatcaa ccagaggaat gcttccattg
      181 ccactatcaa aataagcaaa cgatcattaa atggggctgg aaaaaagtat ccatcttatt
      241 gaatgatgtc agtaactaca agaccattgt gcggattaac aaacagcgct tcaaatgtaa
      301 acactgtgga aagacttttt tagcagaaga ttcagttagc gatcgtcgct gctccattgc
      361 ccgcagagtc aaacaagcca ttcttgagct attaagtgag cctatttcta tgtctttgat
      421 tgcacgtatg aagcacattt ctccgaccac tgtcattcga atacttcgca gccttcggcc
      481 taaaaccgtt tctttgaacc agccgttacc agaagtggtg tgtttcgatg aattcaaatc
      541 ggtgaaaaac gtctcgggag ccatgagctt tgtcatgatg gacggcaaga cccatcaact
      601 gattgatatt gtggaaaatc ggcaactcaa tcctttacgc gactatttct tgcgctaccc
      661 tagaaaagtc cgtgaacaag tacgtctagt ggtttcagat ttctatacac cctatcgcac
      721 attggtgaaa gagtgttttc cgaatgcccg aatcgtcgcc gatcgctttc atattagcca
      781 acacatcggt cgggccttta ccaaccaccg aattcaagta atgaagacct ttaaaaaagg
      841 ggatcgtcgg tcgaagcact taaagaaata ctggagacta cttcaaaaga atgcgtggga
      901 attgaatggt cagcatcgct actggcgtcc gtcatttcga gatcatctga cagaagcgga
      961 gatagtcgat cggctattat attatgatga ctccttaaag cgaggctacg aagtctacca
     1021 ggtttttctt tccgctatca gaagacaaga tgttcctgaa ttcgtcgcgc tcctaaaaga
     1081 agactacaag gagttgccag agcactatca accagtgttc acgaccttca agaaatatcg
     1141 aacagaaatc aaacgagcgt tgcgagttcc ctattccaac ggccctatag aatgtttgaa
     1201 caaccatatc aaagtactaa aacgaattgc ctatggcttt agaaactttc aaaactaccg
     1261 agaacgaatc tttttatatc gaggaaaata tttcaagaaa actaagaaca ctacccaact
     1321 gactaaagcc agaacaacaa cacggctaga caagatagct gtctagccgt gtgagtatct
     1381 cgattattct attgaatttc ttcaccaaca ctcattgacg aagagccctt ttttactatc
     1441 tgtttccaga agctgaggtg ctgctggatg gtgtatggtc atttgcagca tccgctccgc
     1501 tagttgctgg ctcactggaa gaagcaggag gagcagatga agacggtgtc gagctttctt
     1561 cggatgaaga agaaggaacc gttgttgaag acgctggctc gcttgtttct gctgtcgtgc
     1621 tttcttttga ggactcagca gttgattcgc tttgactagt agagctagag ctttgagtcg
     1681 ttgtctctgt tgttgagctt ccaggagtct ggacatagct ggaagatgga atagttgttg
     1741 atggtgtaat tgcattcgat ctagctgtat attgatcttt gtagtacagt tcaccgccca
     1801 cacgaattaa tccgcttggc atctcccagt ctgtatttgt cacgttagct gaaacatact
     1861 gcataagctc gcgataaaca tcagaggcta cgtgtgacga ttctgaagtg acaggtgtca
     1921 tttttttatt gtaacctgtc cacacggaaa tcgaataatt tggcgtatag cccgcaaata
     1981 ggatatctgg atacacccca gaagaaatac caagttttgc atactcgtca tctgtgtaat
     2041 tggatgttcc ggtcttaccg gcttgatata acccagcgat ttgcgcattc gtaccagttc
     2101 cttcagtgat cgtatctttt aaaatgtctg tgatcatata agctgtttct ggagacatgg
     2161 ctgtttttcc atctggctca taagtttctt ctgttccgtc ttggaagacg attttattaa
     2221 catattgagg tttgtaataa gtccctccat tggcgaaagc cgcataggct gctgccattt
     2281 ttaaggaaga agctccgtat tttgtcccat cttgttcttc cgtattacta gagatcgcat
     2341 tcgattgatg gatcgtactg tattcgatcc caagattttt taagaaagaa gccactttat
     2401 ctgaaccgac ttcattgaat aatttgactg ctggaacatt acgggataag taaagtgctt
     2461 ggcgtaaagt aatggtgccc atatattgat tatcccagtt tcctacagga gtcgaagtcc
     2521 cttcatagtt atacggtgcg tcagtaatag tttttccagt agaatatttc aaatattcaa
     2581 aagctggtcc gtaatctgtg acaggtttca tcgtagaacc aaaatcacgg gaagtattca
     2641 ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg acgtccgcca atctgggctt
     2701 ttactttccc ggtattggtg tcgataaggg tggaggcaac ttgcatctcg tcgtctgggt
     2761 aagagacata ctgatccgtg ttgacgatat catatagttt cttttgggcg tccagatcca
     2821 aattcgtata aatctctaaa ccgtctgtgt agacattctt atctgttttt tcttgtactt
     2881 cattgatgac ttctttgaca taattatcga cgatcttagt attgtcatcg gattgagtta
     2941 gttcttgcag accatccgtt accggtacat taacagcttg atcatattct gtttgagaga
     3001 ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg tcttttcttc gcctgatctg
     3061 gataaacata aggatcatag gcggaagggg cttgaggcat cccagcaagc aatgctgttt
     3121 gcggtaaaga aagttctgat aatttcttac caaagtacat ctcagaagct gtctccatcc
     3181 cgtacaagcc gtttgacata tacactttat ttacgtagta ggtcaagatc tcttgtttcg
     3241 attttttttg ttccaatctt acagccatcc acgcttcttg ggccttgcgt ttcagagtct
     3301 gatcctcggc actggtggag aagaaggaaa gtttgatcaa ttgctgtgtc aaagtacttc
     3361 cgccttgcag tccgccagag gtaaagttag agagggcaga accgatgatc cgaatagggt
     3421 caaccccgat gtgtttataa aaacggcgat cttctaccga cacgatcgca tcttctaatg
     3481 ttttcggtaa ttcattcgca gaaatttttt cacgcttttc tgcgcctaaa tcttcgaata
     3541 gttcgccatc ttgtgtatat agtttggaag atacggtagc gtctaatttt ttatccgtca
     3601 attcgggcgc atcttttgcg taataccaga aaagtcctac acctgctaaa aagaggatac
     3661 agaaaaatga gagtattcct aaaaggatct tgataaataa tcctttttta tgtgttccag
     3721 aggactttcc agaacctttg ccagaattct tcttcacact ctttttagga gtaggctgtt
     3781 tctgtcgtct agagcttcta gtttgttcat ttgccattct tgctttctcc ttcaatagat
     3841 aatgttgata aatagaagtc aaccgcgtcc aaataaggaa tccgcggaga aataccagtt
     3901 ttgatctcta ttcctagttc ttcgatcaaa gataaaggta gtgatttgcg tcctttttcc
     3961 ttttgggcgt cccagtaagc aatcaaatga acactattca aataaaaaca gcgcttcaaa
     4021 gacgaaaacc ataacaagac aaagcagacc ccattttgtt cgaggcatgc tttcatatgt
     4081 tcgatttgat gggcatggaa gtttttaaag ggaaaagatg ttttgttctt ggtttctttt
     4141 gcttcaaagt ccaagtaaaa gcctcgatag acgccattat agtctgtagt tgaggcttgg
     4201 gtaaagtagg cttccttgat cacagcagca cttctcttag gatagtctac tttcacgatt
     4261 tggatagggg taggcttttt atggataacc gccaacccgc gggaaagata atatttatta
     4321 ctttcattga ttgcttcttc aaagcgcatc ccccgattgc cgaattcagt gatctgattt
     4381 cttttttctt gaattttttc gacgggagag gcattcttat aataaggttg gccatttgga
     4441 tagcgcaata ccatctttaa tcacactcct aggccacatt atagcaaaaa agcggataga
     4501 aagaaaagga atctgagatg gataagtcaa aagtaatcta tgtgtctggg tatcgaagtt
     4561 tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca agttgttaaa aatgttttaa
     4621 aaaaagaact ccatcagtta gcagaagaag ggctggaatg ggtacttgtc agtgggaatt
     4681 tgggcgtcga actatgggct gtgcaaatag tagcggaatt gaaagaagaa tatccggaac
     4741 tatctcttgg tattttgtat cctttttctg attttggtaa taactggaat gaagggaacc
     4801 aagaaaaaaa acaattagca gaacaattag cagattatgt cgaagctgtt agccatcaac
     4861 cctaccaatc acctagtcag ctaaagaatc acactcgttt tatacttgag catacagtgg
     4921 gctgtctgct gatctatgat gaagaattcc ctggaaaaac gaagtatttt tttgaagatg
     4981 cacagaatta ccaagcagat catccctacg aaattcggct gatcagtatg gacgatcttc
     5041 aaaattttag tgaataatcg atattgtgtt tgatttatca acttttttcc tatacaatga
     5101 aaaaggtaga aatgttatta cgtaaatgaa aactagaggt gtaaatatgg cgaatttggt
     5161 ttatagtcct aaagacattt tacaacaaga attcaaaaca aaaatgcgcg gctatgatcc
     5221 cgttgaagtt gatgaattct tagacaatat catcaaagat tatgaagctt acagcaaaga
     5281 actccttgct ttacaagaag aaaacgatcg attgagtgca aaagtagctc aattatctaa
     5341 aactcaagga gcagctcaaa cacgcgtgca acaaacagag gcaccaaaga gcgcagcagt
     5401 gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa cgtgaagtat ttggtaaaaa
     5461 gcttgatcaa caagcaagtg cagtcaaacc agcacaacct aatccaaata actacacaaa
     5521 tgcagacaca agcttagatg ataatgaaaa aacacgtcaa ttttaatttt tgattaatga
     5581 atttgcggtt ttcgggtaat cgcggtctga cttgatcagg ctgaggaaag tccatgctcg
     5641 cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac cataagctaa ggtactcttt
     5701 ggagtaacgg caggaaaaca agctaaggct tcggctatgc ttaagtatcc ttgaaagtgc
     5761 cacagtgacg aagcggctgg ggaaacccat ccggtggaac gcggtaaacc cctcgagcga
     5821 gcaacccaaa caatggtagg ggcgcttttt gtctggaaga agaacaggac aaaaaggcag
     5881 tataactgca gatagatggt taccgctttg ttttttctcc ctggaaaagc aaagtacaga
     5941 acatggctta tagaaaaccg caaacatcag ggaattaggt cgtgacaaaa gtaatgcgtt
     6001 ataagaaaca agaaaaacat ttgaaagcta tttttcaata cttcagatga atcgttctta
     6061 ttacaaaaca aacttttgta tcaccggtta ttcggaattt gagtaaaagt gtggggtagg
     6121 actgatgtcc cacctccttt ttttataaat aaggagattg tgtatggaaa aaaacaggaa
     6181 gttcaacctc gtcgcaactg cggcaagcgg gctagaagca ctggttggta aagaattaag
     6241 agatcttgga atcgaatgcc aagttgagaa cggacgtgca cgttttgaag gaacaatgga
     6301 aacgatcgct acagctaatt tatggttacg tacagcagat cgagtaaaaa tcgtggttgg
     6361 ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac gtaaaagcat tgccttggga
     6421 agattttctt ccactagatg cagcttttcc tgtagctgga aaatcgatca agtcgaaact
     6481 atatagcaca cctgattgcc aagcgatcac aaaaaaagca atcgttgaac gtctccgtac
     6541 ctattatcac cgtccagctt ctgtaccact gacggaaact ggcgcacatt ttcagttgga
     6601 agtagctttg ctgaaagacc atgtgatggt aactctggat acgacaggac caagtttatt
     6661 caaacgtggt tatcgcttag aaaaaggtgg agcaccattg aaagaaaaca tggcagcagc
     6721 acttattatg ttgacaaatt ggcgaaaaga ccgtccattt tatgatcctg tatgtggttc
     6781 aggtacgctt tgtattgaag cagccttaat aggccataat atcgcaccag gattcaatcg
     6841 tgagtttgct tgcgaatctt gggattggtt cagtcaagaa atcatggaaa atgttcgatc
     6901 agcagccgaa gaaaaagcag attatgatat tgaattagat attactggtt cagatatcaa
     6961 tggacggatg attgaaattg ccaaagccaa tgcagaagaa atcggattag gctcttcgat
     7021 tacctttaag caacaagcag tcaaagactt caaaacggat aaagaatacg gggtcatcgt
     7081 ggctaatccg ccttacggag aacgtctagg agaagaagaa actgttcgga aattatataa
     7141 agaaatgggc gatgtattcc gtccattaaa aacatggagt aaatatatct tgactagcga
     7201 cttagcgttt gaggaatttt acggacaaaa agcaaccaaa aaaagaaaat tgtacaatgg
     7261 tgcattgcgt acggatctat tccagtattg gggaactcgt ccgcctagaa aaaaagagaa
     7321 agacgaataa tcttactaat tgattcattc aattggatgt ttggaggaat agggaatgaa
     7381 agaaaaagag tttttacaag aagtaaaaga aatcaatctt ctacaacaag ctttaggaat
     7441 cctcgattgg gatacacaaa caggtatgcc tgaaaaagct agtgcatacc gaagtgaagt
     7501 agacagctat ctttacagca tttatttttc aaaaaaaatc ggtccaaaaa tcaaagaagc
     7561 cattcgctat ttttctgaac atccggaaga attgtcggaa gttggaactg ccgtttttgc
     7621 caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg gaagatctta tgagaaaata
     7681 tacagctgcc gtttcttcag cacacggaca atggcaaaaa gctagagaaa cacaacaatt
     7741 ttctgatttt caacacgcat tgaatcaaaa tattgaatta acaaaacagt tgatacctta
     7801 ctggagaaaa gatgaaaaga cgaattatga tgttttgctg aatcaatacg aacccgggat
     7861 gactgtcgaa atccttgata aggtatttga tcagcttaaa gaaggaattc tttcgatccg
     7921 ccgtacgctc caagaaaaag ggactgaacc tgaaacagac tttttaaatc ggagaatgac
     7981 gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa caacttggtt atgattttag
     8041 tcgtggtcgt ttagatgaca ctgtccaccc atttatgact ggaattaatc cgaatgatgc
     8101 acgaatcacg acacgatgga acgaaacaga tttcaaaatg gctgtttttg gagtgatcca
     8161 cgaggctggt catggtatgt atgaacaaga catcgatgat aaatatgcgt atacaccggt
     8221 ttatgaaggt acatcaatgg gtatccatga atcacaatcg ttgtttaacg agatcattat
     8281 tggaagtagt cgcagcttct ggaaaaagca atatcctttc tttcaagaat gtgcagaagg
     8341 aacattcgat gatattcctt ttgacaaatt ttatcgttca ttgaaccata caaaatcaag
     8401 tttggttcgt atcgaggcag acagtttgac ttaccctctc catatcatca ttcgttatga
     8461 aatcgaaaaa atgattttca atgaaggggt agatgttgct gaactacctg aaatatggaa
     8521 tcaaaaatat gaagagtatc tgggaatacg accaacaaat gatgtggaag gcatcctaca
     8581 agacgttcat tggtcaggtg caagctttgg ttattttccc tcttatgcat tagggtacat
     8641 gtatgcggca caattacttc attctttgca gcaagaaata gatgtggaaa atgttctagc
     8701 gtcagacgat tattctccaa tcaaaaactg gatgtcagag catatccatc aatatggtgc
     8761 atctagaaaa ccaaatcaat taatcatgga tgcaacacat gaaccgctta atccgcaata
     8821 cctgatcgac tatatgaaag acctgtattt taatgtatat caggtaaaat aaaatacaat
     8881 ttacaaagat agctgaaatt catctaatct atagatgttt tcagctattt acttaagaaa
     8941 gaagtggaac gaatggataa attacaagtg atcgctgatt atagtatgga ccaattagcg
     9001 catgatcaaa caggacatgg gagcgatcat accaaaagag ttgtcaagtt agctgaaaaa
     9061 attttggaga cggaaccgca ggcagaccgc tttgtgacgc ttgcagcagc ttacctacat
     9121 gatacgattg atgataaagt cgttaaaaat gagaacgagg caaagcaaca gcttcgtgta
     9181 ttccttcgct ctttgccaat agtagaggaa cagatctcga tgattttttc cattatcgaa
     9241 aatatgtcct tttctaaaaa tctctctgaa gcggccgaac tatctctgga aggtaagatc
     9301 gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg gcattttgcg tactgcttat
     9361 ttcggcggtg gacatgggca cccgattttt gatcctgaac tgtatccgca aacattcaag
     9421 gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc atttttatga aaaactgttc
     9481 ttgctgtctg ataaaatgaa tactgattac ggatatgaag aagccaaacg aagagaaacc
     9541 ttcatgcgtg aattcttaga agaatttttt ctggaatggt ctattgacga tgaaaatttt
     9601 actccagaca attggatcaa aaataacaaa aaatgatgga ggttgtgaca gaagtggtca
     9661 gctccaagat atttcggatc attttccgta aaagttactt ctggaacact gtttaccaag
     9721 aggtcgtgag cctgccgttc agctccgagt cacaaggaac aatttcaaaa aatagcttct
     9781 cgtgtttgat gaatattgta acttgtgatt cggaattggc aggagaacac cgtttattcg
     9841 gaagaatagg actgtgacaa gacttttgtc acagtccttt tttcgaattc aacaagtaaa
     9901 acttttaaga aatattcaga gagtaaaatt aaaaattgct gataaacgaa gatggaaatt
     9961 tta
//
LOCUS       DAZKBH010000099         9828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000099 DAZKBH010000000
VERSION     DAZKBH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.100"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..684
                     /locus_tag="ACG8OO_002292"
     CDS             205..684
                     /locus_tag="ACG8OO_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFP9046140.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="ACG8OO_002293"
     CDS             717..2945
                     /locus_tag="ACG8OO_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9046141.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="ACG8OO_002294"
     CDS             3074..4717
                     /locus_tag="ACG8OO_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9046142.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="ACG8OO_002295"
     CDS             complement(4790..5335)
                     /locus_tag="ACG8OO_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFP9046143.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="ACG8OO_002296"
     CDS             complement(5358..5843)
                     /locus_tag="ACG8OO_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9046144.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="ACG8OO_002297"
     CDS             complement(5833..6645)
                     /locus_tag="ACG8OO_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFP9046145.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="ACG8OO_002298"
     CDS             complement(6784..8316)
                     /locus_tag="ACG8OO_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFP9046146.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="ACG8OO_002299"
     CDS             complement(8425..9456)
                     /locus_tag="ACG8OO_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9046147.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3270 a         1828 c         1892 g         2838 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtat
//
LOCUS       DAZKBH010000100         9641 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000100 DAZKBH010000000
VERSION     DAZKBH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.101"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..292
                     /locus_tag="ACG8OO_002300"
     CDS             98..292
                     /locus_tag="ACG8OO_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046148.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            561..860
                     /locus_tag="ACG8OO_002301"
     CDS             561..860
                     /locus_tag="ACG8OO_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046149.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(957..1391)
                     /locus_tag="ACG8OO_002302"
     CDS             complement(957..1391)
                     /locus_tag="ACG8OO_002302"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9046150.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1623..2333)
                     /locus_tag="ACG8OO_002303"
     CDS             complement(1623..2333)
                     /locus_tag="ACG8OO_002303"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9046151.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2323..3183)
                     /locus_tag="ACG8OO_002304"
     CDS             complement(2323..3183)
                     /locus_tag="ACG8OO_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP9046152.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3188..3826)
                     /locus_tag="ACG8OO_002305"
     CDS             complement(3188..3826)
                     /locus_tag="ACG8OO_002305"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9046153.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3841..4140)
                     /locus_tag="ACG8OO_002306"
     CDS             complement(3841..4140)
                     /locus_tag="ACG8OO_002306"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9046154.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4183..5667)
                     /locus_tag="ACG8OO_002307"
     CDS             complement(4183..5667)
                     /locus_tag="ACG8OO_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9046155.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5687..6148)
                     /locus_tag="ACG8OO_002308"
     CDS             complement(5687..6148)
                     /locus_tag="ACG8OO_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9046156.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6166..7233)
                     /gene="ulaG"
                     /locus_tag="ACG8OO_002309"
     CDS             complement(6166..7233)
                     /gene="ulaG"
                     /locus_tag="ACG8OO_002309"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP9046157.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7451..8221
                     /locus_tag="ACG8OO_002310"
     CDS             7451..8221
                     /locus_tag="ACG8OO_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9046158.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8494..9321)
                     /locus_tag="ACG8OO_002311"
     CDS             complement(8494..9321)
                     /locus_tag="ACG8OO_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9046159.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         3004 a         1961 c         1505 g         3171 t
ORIGIN      
        1 gattcttttt atttatcata aagatataga tacataatca ttttcactta catatatttg
       61 aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag aagtaaaaac
      121 aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc ctttacttgg
      181 ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa agaacgagct
      241 agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt aaatccaatg
      301 tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt atagaattag
      361 aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac catatgatta
      421 tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg aatcaaatat
      481 acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta taatgtatca
      541 gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa ctacttaagc
      601 aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa gtgacagagt
      661 atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca aaaggaatat
      721 acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct ttaaaaaaaa
      781 atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt gataaagagg
      841 tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac taagtctatc
      901 ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat tatatgttat
      961 ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga aataccaccc
     1021 atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt ctcagtatca
     1081 tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa agcttcattc
     1141 aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat atcttgaata
     1201 aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt cccttcccca
     1261 ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc agtgtctttt
     1321 gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat cataagggaa
     1381 tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat acagttcttt
     1441 gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc cttaaaattc
     1501 ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata aattttcttc
     1561 acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt accctagcat
     1621 ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt atctagtaaa
     1681 tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc catcagacaa
     1741 atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc ccatgtgaac
     1801 ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt tctttcctta
     1861 aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac ttcttctttc
     1921 gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt cgtgccataa
     1981 gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg agtatgagta
     2041 atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag atctgaagat
     2101 ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac catttgatct
     2161 gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc tagctcacgg
     2221 tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg caatgccaga
     2281 tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga catcatagcc
     2341 agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc ttcgaaagtc
     2401 ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta gacgtttcaa
     2461 atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat cacgaaactg
     2521 gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag tgatataatc
     2581 gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca aattgcctag
     2641 gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta aaaattttga
     2701 aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc catatttaga
     2761 agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat tcattgattt
     2821 ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta gattatgcgc
     2881 caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg gatcagcaga
     2941 accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta caccggagct
     3001 gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc gtgctaaccg
     3061 ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt ttacaaggga
     3121 aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc caatttgagc
     3181 catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg gccttcatcg
     3241 gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg atcccttcaa
     3301 ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag cctagttcaa
     3361 tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat aacagcgcat
     3421 cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc atggcttgtt
     3481 caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt tgtgctgcct
     3541 ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct gcttttgcaa
     3601 cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact agttttttat
     3661 ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag caaagaattg
     3721 tgcccacttc aataatatct actacatcac caactttcat aacatctcct aatgcatgtt
     3781 ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta gttccttcct
     3841 ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt ctttgactga
     3901 catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag taaacatatt
     3961 caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt ttccctctga
     4021 aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg ctttttccac
     4081 acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta aaattctcat
     4141 cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc tctgcttttc
     4201 tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt tccgatattg
     4261 taactgcgga atagccaata aaccaatgac aactacggca attccagcat aacctaggac
     4321 tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc cgatataacc
     4381 accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta cttgtatgat
     4441 tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat tggcataaac
     4501 tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga tcgttggtga
     4561 tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa acaagaaccc
     4621 aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa ttcctggaac
     4681 agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt catcgacgaa
     4741 tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag aagtcgtcat
     4801 aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca aataatcttt
     4861 gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag tagcaaccat
     4921 attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt ttttgtcttg
     4981 cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta attttgcaaa
     5041 tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt ctgtcaattc
     5101 ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta aaatcagtcc
     5161 cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta aaatccaaaa
     5221 agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac gcagtttcgt
     5281 atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta ataaaatcat
     5341 cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag cattttgtcc
     5401 aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc caaccaagat
     5461 tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt aaccgaccgt
     5521 tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa tatagcctaa
     5581 taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg caaaatacgt
     5641 ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa ttatattctg
     5701 ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca accaatccat
     5761 ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct ggatcttttg
     5821 ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca ctttcaaaac
     5881 ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca tcactttttt
     5941 ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg tatttttcta
     6001 cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg atcaatacaa
     6061 gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg taatgaatca
     6121 attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata aaaacgacgg
     6181 aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa aattaaactc
     6241 aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga tataaggttt
     6301 aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct cttttggatc
     6361 agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg cattcaatga
     6421 ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc gtgggttttc
     6481 accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg caaataaatt
     6541 ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga ataaatagtt
     6601 tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt ctggatcttc
     6661 acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga tgtctttgat
     6721 tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc cccatttttg
     6781 ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat tttgatgaac
     6841 tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga ttaaagcgtc
     6901 tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat taggctgcat
     6961 attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc cattcccatg
     7021 actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt catgactttt
     7081 cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat ccactacttc
     7141 tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta ctaaccactt
     7201 ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat atcgttgttc
     7261 caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt atatgaccgt
     7321 ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa taaagacaga
     7381 tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg tccctcagga
     7441 ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag aaattttgga
     7501 actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc ttgatgtatc
     7561 tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga tcatacgcaa
     7621 ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat ctggtcctga
     7681 aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt atgatacgct
     7741 gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat ctaaccattt
     7801 gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata attgtagtta
     7861 tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg acatcgcatt
     7921 aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg gagtcgatgt
     7981 agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa tgatggtcaa
     8041 caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac tgacatcaaa
     8101 gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat tttgttctga
     8161 aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag attcatttta
     8221 agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca aatttatcga
     8281 taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta tccatccaat
     8341 cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta aataggaatc
     8401 aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc gaataaacgg
     8461 tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt caatcacctt
     8521 taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat atctttctaa
     8581 ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca agtattccaa
     8641 tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata attggattgc
     8701 tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata caatgcagac
     8761 catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca cataaacttt
     8821 cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag ctgcttgtaa
     8881 gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa ataatgctgc
     8941 atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa tttgatttaa
     9001 aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat aagattcttg
     9061 gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga ttttgtcttc
     9121 ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa gcgcacttaa
     9181 gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg cttgttcaca
     9241 aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt cttttagtaa
     9301 tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt tctcctttat
     9361 atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat aaaaatttta
     9421 attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt tctttttgtg
     9481 ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca tggcgagaaa
     9541 gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat gacgacagca
     9601 cacctatcaa ttaatataat tattttattt ttttatgttc t
//
LOCUS       DAZKBH010000101         9357 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000101 DAZKBH010000000
VERSION     DAZKBH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.102"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8OO_002312"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8OO_002312"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9046160.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8OO_002313"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8OO_002313"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP9046161.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8OO_002314"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8OO_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9046162.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8OO_002315"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8OO_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9046163.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8OO_002316"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8OO_002316"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9046164.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8OO_002317"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8OO_002317"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9046165.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8OO_002318"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8OO_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFP9046166.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8OO_002319"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8OO_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFP9046167.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8OO_002320"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8OO_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFP9046168.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
BASE COUNT         2355 a         2095 c         1621 g         3286 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttt
//
LOCUS       DAZKBH010000102         9262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000102 DAZKBH010000000
VERSION     DAZKBH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.103"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..298)
                     /locus_tag="ACG8OO_002321"
     CDS             complement(<1..298)
                     /locus_tag="ACG8OO_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFP9046169.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTF"
     gene            complement(288..2462)
                     /locus_tag="ACG8OO_002322"
     CDS             complement(288..2462)
                     /locus_tag="ACG8OO_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFP9046170.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2478..4820)
                     /locus_tag="ACG8OO_002323"
     CDS             complement(2478..4820)
                     /locus_tag="ACG8OO_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9046171.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4842..7385)
                     /locus_tag="ACG8OO_002324"
     CDS             complement(4842..7385)
                     /locus_tag="ACG8OO_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9046172.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7395..7793)
                     /locus_tag="ACG8OO_002325"
     CDS             complement(7395..7793)
                     /locus_tag="ACG8OO_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFP9046173.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7874..8158)
                     /locus_tag="ACG8OO_002326"
     CDS             complement(7874..8158)
                     /locus_tag="ACG8OO_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046174.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8182..9180)
                     /locus_tag="ACG8OO_002327"
     CDS             complement(8182..9180)
                     /locus_tag="ACG8OO_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9046175.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2632 a         1989 c         1449 g         3192 t
ORIGIN      
        1 aaaaagtttg aagttcttct tgtcttggtt cttttcttgt ttcaattcga tatacattcc
       61 tagagaattt tttgtcgtat ctttccaaga aagattcaac ttcttggcat tttttacaat
      121 catttcttgc tagatacaca aaaaatgatt gattcgtttt ttgaagttct agtacttctt
      181 ctaaattact attgattagt tttccatttg attcttcgtt agtttttatc agtttaaaac
      241 aaccaaataa gaggaaacta acaataagta tcctaacaat aactttctta tttcgcatat
      301 tgtttctcca aatttggttt ccgaatatag acgactcgcg gtggttcctg gacgtcttca
      361 gctgttcgat acaccacaaa ttcgcctgtt ctcaatgctc gaatgcgatt cggattgaat
      421 ttaaagcggt ctacgtctcg tttcgtgcct ctttctcctg tccaatcaac ctttcccata
      481 tcagaataac ctgcttcttg tgtcgtcatt tccgttaatt caatgtcttc ttctgtcccc
      541 attaaatctg cccaataatc aatttctgtc gggtcatttg tcttcccaat aaagaacgta
      601 ttcacattcc caatcaactg ttttcgaagg acaggatcaa ttctatcaat gtccgcaagc
      661 gtttgagggg catacgccgc atgcacccca gcgcctcgtc ccttattgac taagtctata
      721 aggttatcat ttagataaga agctggttcg tcaaatatta ctaggacttt cgagcgattg
      781 gctgtttctc ctgcttgttt atacacttct gacgcaaaga aattaatatc tcccacaagc
      841 atttgagcca atgtccgaat aaactcacta taaatcaatc cgtttaacga gatataaaca
      901 atctttcctt ctttaacaac ttcttgaata tctaattctc tttttcctcc agaagtatca
      961 aagagttcac caagctcact atccaataac atattgatat tggtctgtaa attctcagaa
     1021 gtcgcataca aataaaacgg gttcttctta tgttcgtaac gaacaaacaa ccgtttaaac
     1081 aacgtttgct cgatttctgt catgagagaa atgttttctt ttaataatag ataatacgtt
     1141 tctaagtcaa cggttttacg atcatgttca acgattcgct cttcacttaa ctgttccaca
     1201 tcggcaaaat cacgttctgt tttgtccatc gcttcattct ttttcgtttt tttcttcgtg
     1261 gttttcttct tttctacgac cactttttct ttaaataaat ctggattttt caactgaata
     1321 cgatccgcaa acaaatgtaa gacgtttcgt ggcaataaat aatgttgtaa ccaaactaga
     1381 tttctttcaa ttttatggct cttttggaac tcgtctaaaa gctgaatcgt tacctgtaat
     1441 aacgctttgg ctgctgtcga ataatacacg ctttctgttt ctgctagatt ggttaattta
     1501 tctcgaatgg caatatcatt gccatttttt acaggattat aacgcaaatc gccaccgtcc
     1561 acaaagactt caatcgggcg attgtatgcc tctgccactt cttttaacgc ttgtaaagtc
     1621 tctcctgctc ctttcccatc gactaagata acggggatac gaaacttaca cgcatgttct
     1681 gctagtaatt ggatcaccgt cgtttttcct gaacctgtag tcgctggaat aatcacatgt
     1741 tggttcaatt cactaaagtt catcacataa gggagcccat actcatctgt tcctaataga
     1801 atcactttgg ctaatgaagc cagttgttta ttccgcaaag caacgtcttt tatgacaatt
     1861 gctacctggt acgcttgctg atactctgct gtatattttt gggactcctg aaatttattc
     1921 ttccgacgtt ccttgtattc tttactattt agcaaagctg tttgccgttc tttctttgaa
     1981 acaacttttc ttttttcaat aaaagacgca tacgcaatca aacaacttcc tgcacctgtc
     2041 ccacctaaaa ggagggcaaa aatcgtttgt ccattaaact gcaacgcatg agaaaacaag
     2101 tttaatggaa ataataagta acacgacaaa taccctctag gaagaacaaa aataaaacaa
     2161 aatagattta atagcgtaaa acctaacgca aaaataaaaa aaagagagct tcttcgtttt
     2221 aagaagctct catactcaaa gtcataaata tgtgcacgtg tctctttttt atctatcata
     2281 aagacaatcg ctaaaggaat actgcctaca ataaaaaatg gatatagaca aacacctaat
     2341 aaagtgacga taaaataaag ttgttgaaac cgactttttg gcgtttcttt ttcttgtttt
     2401 cttttgcgat cgacagaaaa catttgctca aaatgtgtcg gtcgttttgg tgcattcatc
     2461 atactatcga tttcctccta tcgttttcgt tgtttcattt ctcttcgata aatcggttgt
     2521 ttttctcgtt ttcgtttcat ttttgcttga gttgctcgaa actcactccg tgtcttcggt
     2581 gcttgttttg gtgattttgt ttgtttcatg gttgagtatg ctttagatac ggttgaaggc
     2641 gctttggttt gtgctgttac tcgattgact gctgacatat gttcccgaac cccctctgga
     2701 cgtcgaacag atttcggtgt ggtagaacct gttgctcgtg gtacattccc ttttcttagt
     2761 ggtttttgta ccgcatgttt ttgtcctgca actgtccggg ttttcatcga acttgttttt
     2821 gttacgggag taagtgcctc cttccgacga cgacttgttt gtccctgacg acgttttcga
     2881 tcgttcaatt ctgttctagg cgtatgcttc tggccataag acttgtgttt ttgctttgga
     2941 ttcgctaatg acaccaccat tgcagaaccc tgattgcgat acggactacg agacgcacct
     3001 tttctacttc tttctcttag actaggattg ccttgcaatt ctcgttgtaa taaatcggct
     3061 gtttttcttg cctgtttctc cattgctgtt cgttgcatac gttcttgact ttgctcttct
     3121 gcttgtttcg cttgatcatg caagtctctt gctttttgtg ccatacgttc catacgattt
     3181 ggtgctttat gacgaggttg ttgtgcggat tcacgccgaa aagcacttcg tgtggcttgt
     3241 ttcgccgttt gtggcatacg aacgtctgag ccacccccac gttttcgttg acgttcttgt
     3301 ctcttccgtg cgttttcttt ttctttttgt ttctgtgctt ttctcgcttc tttgcgttct
     3361 ttcaaccgtt gtttggcttc ccttctcaat tttgcttctg ccccaaatgg atttcccgca
     3421 aaagctaacg ctcctcgaac acccattctt ccagtaaata gactgcctaa aaagcctcgg
     3481 aacaaataaa ttcccaacgg cgcaaacaaa taaacaatga tcaagagaat tttttcccat
     3541 ggatcgtctt gtgtactact taattggaac ccgtaactaa gaaaagaaga aaaaaggata
     3601 catgcaaaac ctgtcattcc ttttaaaaac gtcaaaatac caaaaacttt ggcatagtta
     3661 ccaaacacat tcattgtgac aagaaacaat cctaaaaaaa gtaagaacgg caacatcggt
     3721 actaacaata gttgcatagt tccaataatc aatcgaaaca tacttaagac aaaataaaca
     3781 acggtttgaa tcagattcac gacaaaataa aacaaggcaa atgctgtatt tttccaacta
     3841 ttgtaatatt gtatgttctt gtttttcagt gtatctgctt catactctgt gactttgtca
     3901 tgaattttct cccctatttt tgtacctaaa tcattgtcca ataaaagatt gattcgatca
     3961 tattcttttc cagcatattt gactttcttc tcacgaattg tgtctacatt ggttgtgcca
     4021 taatttaaca acagatatgg ttcataaaca ttactataaa agacccgagc cgccatcaat
     4081 ttgcctgccg tttccacttt tttatccgct gttacctctt ctgtttcttt tgtttcataa
     4141 tttgtttctt cttcaggtgt taacactgga ttgaccttca cgaacgccgt ttccactgta
     4201 gaatctaaag aaaaaagaac atcaaaaaga ttcttatttg tcccagaatc ttttaatacg
     4261 cttaacccag taaaaataag gatcgtcata agaaagacac ggagtgcttg tttcattcga
     4321 gcctctccag catatttgat tgccatcata attgccgaaa aactgattcc aatagttcct
     4381 gcaatactta acatattgtc cgcaattttt tgtgttaagt tttgaatggg ttctcgaatg
     4441 gcggaaataa tatctaattc aaagagaaat tcaaccattg aagcattgaa ttctcctaac
     4501 atttttacgc cactccaaac aaaatttttc atcactcctg caacattgta cgatagcttt
     4561 tttttaaggt tcattgactc atagtcattg gtcatcaatt caaacgaatt ctttttgtaa
     4621 tttttataca attgatccac atcagtcgtt acttctttta cttctacttt atcgttggca
     4681 ttctctcctt cagaacgatc ggtttcagtg acacccgcac catcattcat ttgatcggca
     4741 gaaacagtag ccccttgtag acagaaaaaa agacccccta ataaaaggag cgtccaccat
     4801 ttcagagatt tttttcgcaa aaaaaccacc tcttttttgt tttatcgttg tttttcattt
     4861 tctaacattg cttgaatttt ttcatcttga ttttcagtag aagaaacagc caataaccaa
     4921 ctatcaaaca acacatcaat ggcaatcggt tgatttcttc ccaaatgatc ctggaataaa
     4981 caggttcctg tttgtaattg ttccatcatt tctaaattgg ctgaattcac ttccatatca
     5041 aagaaggaca atagtttctc tcgtgcttct ttttgtttcg gacgaaacgc aaatttgtaa
     5101 gaaattaact cttttttatc ttcttgatcg taatctttaa acgcttgcac aacaaggtaa
     5161 atatccgtat tattcgcacg tccttttcgc aagctatcat caatcaaata gcttccttgt
     5221 gcggtatctt catacccttt tgcctcgtca taaataatcg cagcgtcttc ttctttgttt
     5281 gtagaaaaga tatacgtaaa cttcataatg acttccataa ttgccattcc tgcaagttgt
     5341 tcgctattta atcgacccga atcaatctct ttttgtgtcg gaatgcgtat tccttgtgtc
     5401 cctaacacgt tgatttgtga atcaaaacgt atcggcgtac tgtgctcatt cccaatgaga
     5461 atctttccta aaccaacgct atgccctaaa atcaatttag ctagttcttt gtcacttttt
     5521 cgaatttctt cgataacttg acttaacgaa tgcttacctg gcatggtgta aactacatcg
     5581 atggcgtctt ggatcaccgt tttcttgtgg ctcgctgttt ctcgattggt atctacttct
     5641 cctaacattc ctaacacatt tttagcggtt tgaattgcct cttcttttgg taaaaacaat
     5701 agaggatcaa gcatccctcg acagctttca tctgaagata acgaaataaa attaattcgt
     5761 tcatacatag ccacaaactc tggtatgtgt ccgaattttt caagtgcctt taaaaagaac
     5821 ttttgggttt catttttcgg atcaatataa agaatttttt gacctaaaaa catcagccat
     5881 aaaaaaatat attttacaag gaccgatttt cctttacctg gtggaccaat aatcaacgta
     5941 tttccattgg tatgttccgc gccttcaata cttttctttg ttaaattggg ataataccat
     6001 actagtttac tagatagcgc taacgcttct ttcgcagatt taaacttttt tgcaataaca
     6061 atccgtccta aaggaaatcc tttcttattt cctacttctt tctttaagtc caagccataa
     6121 tctgccacaa aacctgtcgt agcgacctgc tcgtaactac gaaaacgttg cgtacttcca
     6181 attaagcatt gattaaacaa cgttagctga tccgctaccg atcgataaaa tttgaaatct
     6241 gtagactcaa tcgcaaattc aatatctttg actcgttctt ccaattcttc ttttgaagaa
     6301 gcagatacaa ccagcgtgac ggtagaataa cacaatcgtt tttctttgcg cttcagctta
     6361 tcttttacat ccattaaccg ttctgtacca aaatttaata catcgtctga atctaaaatg
     6421 ccgtcaacgg tctcatattc tttttcttgt tcataaatcc gtccagccat tttattaatt
     6481 tttcgttcat cagattttga atgctcaaat cgaagtcgaa tatgcgtatc aattggaaaa
     6541 gataagctat cctgaagatt ttgaataaag ctactcccat acatggctaa aggagcgtcc
     6601 gtttgactta aaaacgtcga ataatgcgtt tgattcaact gttcaatcgc aatgtatcct
     6661 gcttcattcg aaatgacgcc ctcgcaaatt tccattggcg taagctgtct ttcaggcaat
     6721 ttttcttctg atcgatggaa aaaataataa taaatttgtc caatctcttg ggcagataag
     6781 cgctctatcg gcttataatt ggacaattcc gtatagagct ccttttctaa tgtttcatac
     6841 gttggtaaca cactacttat aggaactcgt tgtcccgtaa tacgatagat aaacttttta
     6901 aaaacatgct taagcaattg aaaatattcg atcggatcag acactttcat ttctgtagtg
     6961 aaacgcacaa acagatacgt tcgatactcg tttaattgca cttcgtcttt taaaatcttt
     7021 cctgcctgtt caaagtacac tttcccaaga tctgcgcatt ctccctttac caaatctcga
     7081 atggtttctt caatgtgcgt atctaggtta aattgacgtg gaatattgat aaaatgatag
     7141 tcatattcat catgtccaag tatgccttcg ccatcttgaa gatagttctt gaaaaaatca
     7201 atgtcattta aaggaaatac ttgggaagaa agttgatagc ctacccacac atcatcattt
     7261 ttggtcaaaa caaaatttcg gataatggcg tctatcggaa attctaatcg aggaagcttc
     7321 gtttttttct ttttgaactg ccaaatcccg ataataaaga cgaccaaaag aaacaaaaac
     7381 gccatttact cgactcactc cttccatttg tgtagggaat acgtcacttt ttcatggaaa
     7441 aacggcacat acatttcatg ttcaaagcgt tgtttccgtt tctgataata ggacgttcgc
     7501 ccaattaaat actcgaaaaa gtgtttattg tcccatttca ttgaaaacaa tgtccaaacg
     7561 agaacaccaa tcccaaaaat cataatcaac caggactttc gaaacagtgt ctgcaagaaa
     7621 ctaatttgat tcacatacgc aatcaaaaat aggatcaaaa gaatgaagag aaatacccca
     7681 cctatgacta attgttccat gcgaatgcca ttagaaatcc agggaatttt ttgtccttta
     7741 atcccgtaaa ttttgtgagg gtgcttaaac tcctttgtat agttgtattc catgttcact
     7801 ctacctcctt ttacaaaaaa agaagcgacc agaagttcta atcttctgat cgcttttgca
     7861 gtttgtcttt ttcttatgat aattgtatcc agggcacaat catttgcgtt acccccacgt
     7921 tctcttcact atccttaata aagataatgt tctttagcca ttcaccaata tcaacggcat
     7981 tcgataacaa taagacgagc attatcaaaa taaagacgcc aataatggca aacgctgccc
     8041 gcgcaaagcc ttgggtcgca taagcaccaa ccattagaac aatcgcaacg attgccaata
     8101 gccaaatgac ttgtccagat acatcatcaa ataacgattt aagatcaaaa agtttcattt
     8161 ccattctcct ctaagttcat tttattcatt taacgtagta acaaaataac tatcgccact
     8221 tgtttttact tccattgaaa agtttgattg gaactcgttt cctttctcaa aatagaaacg
     8281 atacttccct tgcaaaaagt agtttccttc tttatttttt actgcatgat ccagttgtac
     8341 attgatcaat tcgccgcctt ctaacccttt ttcgttagaa atcaatgcca atttttcatc
     8401 atttttgaca tataactcaa aaaaccgttt cacaaatgct tccagcttga tttcttcgct
     8461 ttcagtgacc gcttgcccag aagaataaaa tcgattttcc tgatagttca cagaattggt
     8521 attcttactc tcagaaagat tcaaaaattg aacgccatca agtactttta aaacattgtt
     8581 ttgataactg actggaaaag aaagttgact gcttttccaa tgttctcctt ctgtaaaagt
     8641 aacctcataa aataggcgat acattgcttg tgtcaaatcg atttcttcta gtttaataaa
     8701 gcgaacatct ttcgctactc gattggtagc cgtcgttaca aagcctagat cagacgcttc
     8761 caatccttgt gctaaataat gattgacgga agcctcccat tcgtttcgtt tttctggcga
     8821 ataagtaaat aacctttcaa taaaatgttg tccctcgtac ttcaattgtt ccgtagaaga
     8881 caaggtttgg ttgacttcag ttaacaatgt ttctggattg acttgttttt tcactgctaa
     8941 acgcgttaat gtatccactc gtccaaagtt taggaggagc acgacaccca ttaacagaaa
     9001 caaacaaaaa aaagcgcctg aaaatacact catggcactt ttttgcgaaa attggatcat
     9061 tcgatctgac ggttctgttt tctttttttg gaacaaaaaa cgtttctttt gtttcttttc
     9121 cttctgttgt ttcggtggct tagtttggaa cgcttgtgga ttaaattttg cattactcat
     9181 gagaaatgcc ataccgttct aacaataatt tcggtatttt gtccacaatc actacttcgt
     9241 ggacaaccac acttgccccc tc
//
LOCUS       DAZKBH010000103         9169 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000103 DAZKBH010000000
VERSION     DAZKBH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.104"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACG8OO_002328"
     CDS             136..309
                     /locus_tag="ACG8OO_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046176.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACG8OO_002329"
     CDS             949..1422
                     /locus_tag="ACG8OO_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFP9046177.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACG8OO_002330"
     CDS             1431..1658
                     /locus_tag="ACG8OO_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9046178.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="ACG8OO_002331"
     CDS             2294..2665
                     /locus_tag="ACG8OO_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046179.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="ACG8OO_002332"
     CDS             complement(2921..3163)
                     /locus_tag="ACG8OO_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9046180.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="ACG8OO_002333"
     CDS             complement(3195..4097)
                     /locus_tag="ACG8OO_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9046181.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="ACG8OO_002334"
     CDS             complement(4110..4298)
                     /locus_tag="ACG8OO_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9046182.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACG8OO_002335"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACG8OO_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9046183.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="ACG8OO_002336"
     CDS             complement(4913..5806)
                     /locus_tag="ACG8OO_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9046184.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="ACG8OO_002337"
     CDS             complement(5884..6807)
                     /locus_tag="ACG8OO_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9046185.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="ACG8OO_002338"
     CDS             complement(6856..7206)
                     /locus_tag="ACG8OO_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9046186.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ACG8OO_002339"
     CDS             complement(7239..8141)
                     /locus_tag="ACG8OO_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9046187.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ACG8OO_002340"
     CDS             complement(8134..8991)
                     /locus_tag="ACG8OO_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9046188.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2570 a         1736 c         1508 g         3355 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtat
//
LOCUS       DAZKBH010000104         8675 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000104 DAZKBH010000000
VERSION     DAZKBH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.105"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACG8OO_002341"
     CDS             <1..1101
                     /locus_tag="ACG8OO_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9046189.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACG8OO_002342"
     CDS             complement(1280..1615)
                     /locus_tag="ACG8OO_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9046190.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACG8OO_002343"
     CDS             complement(1599..1862)
                     /locus_tag="ACG8OO_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFP9046191.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACG8OO_002344"
     CDS             complement(2322..3125)
                     /locus_tag="ACG8OO_002344"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9046192.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACG8OO_002345"
     CDS             complement(3103..4227)
                     /locus_tag="ACG8OO_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9046193.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACG8OO_002346"
     CDS             complement(4243..5058)
                     /locus_tag="ACG8OO_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9046194.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACG8OO_002347"
     CDS             complement(5048..5920)
                     /locus_tag="ACG8OO_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9046195.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACG8OO_002348"
     CDS             complement(6005..7294)
                     /locus_tag="ACG8OO_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9046196.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7971)
                     /locus_tag="ACG8OO_002349"
     CDS             complement(7438..7971)
                     /locus_tag="ACG8OO_002349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046197.1"
                     /translation="MANEKATMTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQL
                     SWAAIWGKHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAR
                     EVGQQLGLTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGV
                     KRFGDGIHDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACG8OO_002350"
     CDS             complement(8017..8160)
                     /locus_tag="ACG8OO_002350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9046198.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DAZKBH010000105         8572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000105 DAZKBH010000000
VERSION     DAZKBH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.106"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="ACG8OO_002351"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="ACG8OO_002351"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9046199.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1590..2936)
                     /gene="gor"
                     /locus_tag="ACG8OO_002352"
     CDS             complement(1590..2936)
                     /gene="gor"
                     /locus_tag="ACG8OO_002352"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9046200.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3070..4323)
                     /locus_tag="ACG8OO_002353"
     CDS             complement(3070..4323)
                     /locus_tag="ACG8OO_002353"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9046201.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4329..5252)
                     /locus_tag="ACG8OO_002354"
     CDS             complement(4329..5252)
                     /locus_tag="ACG8OO_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9046202.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5473..7965)
                     /locus_tag="ACG8OO_002355"
     CDS             complement(5473..7965)
                     /locus_tag="ACG8OO_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFP9046203.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7972..8439)
                     /locus_tag="ACG8OO_002356"
     CDS             complement(7972..8439)
                     /locus_tag="ACG8OO_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFP9046204.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2354 a         1814 c         1422 g         2982 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
     1081 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
     1141 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
     1201 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
     1261 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
     1321 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
     1381 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
     1441 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
     1501 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
     1561 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
     1621 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
     1681 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
     1741 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
     1801 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
     1861 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
     1921 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
     1981 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
     2041 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
     2101 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
     2161 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
     2221 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
     2281 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
     2341 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
     2401 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
     2461 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
     2521 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
     2581 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
     2641 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
     2701 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
     2761 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
     2821 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
     2881 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
     2941 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
     3001 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
     3061 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
     3121 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
     3181 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
     3241 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
     3301 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
     3361 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
     3421 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
     3481 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
     3541 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
     3601 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
     3661 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
     3721 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
     3781 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
     3841 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
     3901 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
     3961 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
     4021 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
     4081 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
     4141 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
     4201 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
     4261 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
     4321 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
     4381 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
     4441 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
     4501 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
     4561 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
     4621 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
     4681 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
     4741 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
     4801 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
     4861 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
     4921 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
     4981 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
     5041 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
     5101 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
     5161 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
     5221 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
     5281 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
     5341 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
     5401 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
     5461 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
     5521 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
     5581 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
     5641 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
     5701 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
     5761 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
     5821 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
     5881 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
     5941 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
     6001 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
     6061 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
     6121 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
     6181 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
     6241 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
     6301 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
     6361 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
     6421 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
     6481 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
     6541 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
     6601 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
     6661 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
     6721 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
     6781 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
     6841 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
     6901 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
     6961 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
     7021 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
     7081 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
     7141 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
     7201 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
     7261 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
     7321 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
     7381 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
     7441 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
     7501 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
     7561 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
     7621 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
     7681 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
     7741 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
     7801 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
     7861 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
     7921 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
     7981 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
     8041 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
     8101 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
     8161 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
     8221 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
     8281 ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag
     8341 gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat
     8401 atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta
     8461 atattgacct taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa
     8521 ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAZKBH010000106         8513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000106 DAZKBH010000000
VERSION     DAZKBH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.107"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1248
                     /locus_tag="ACG8OO_002357"
     CDS             94..1248
                     /locus_tag="ACG8OO_002357"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFP9046205.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="ACG8OO_002358"
     CDS             complement(1270..2022)
                     /locus_tag="ACG8OO_002358"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9046206.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="ACG8OO_002359"
     CDS             complement(2386..5052)
                     /locus_tag="ACG8OO_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9046207.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="ACG8OO_002360"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="ACG8OO_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9046208.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ACG8OO_002361"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ACG8OO_002361"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9046209.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            8282..>8513
                     /locus_tag="ACG8OO_002362"
     CDS             8282..>8513
                     /locus_tag="ACG8OO_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046210.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEK"
BASE COUNT         2525 a         1746 c         1505 g         2737 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8161 tcgctattct aataataaat aaccactatt tgatttatat atatttcagt atccttcttg
     8221 ttttcatata accaaaggcg ctatgatttc catatgaaaa aaataaagtc aggtgaaaaa
     8281 aatgaaactt gaggataaaa aagtcatttt gaagaaaatc aaacgactac cagaccaaat
     8341 agatggctat acgatctgtt ctttcaaaaa agacaggagt ataaaagctt atacgcaaaa
     8401 cacagaaaaa ggaataatct atttcctaca tgaagaaggt tacgataccc aaacctttat
     8461 gtttactgaa aaaaaagaat ttcataaaca gatgaaaaaa ctgattgaaa aag
//
LOCUS       DAZKBH010000107         8494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000107 DAZKBH010000000
VERSION     DAZKBH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.108"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8OO_002363"
     CDS             complement(<1..149)
                     /locus_tag="ACG8OO_002363"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9046211.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8OO_002364"
     CDS             complement(593..1714)
                     /locus_tag="ACG8OO_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFP9046212.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8OO_002365"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8OO_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFP9046213.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8OO_002366"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8OO_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFP9046214.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG8OO_002367"
     CDS             complement(4304..5215)
                     /locus_tag="ACG8OO_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9046215.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG8OO_002368"
     CDS             complement(5232..6239)
                     /locus_tag="ACG8OO_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFP9046216.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG8OO_002369"
     CDS             complement(6236..8365)
                     /locus_tag="ACG8OO_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9046217.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG8OO_002370"
     CDS             complement(8370..8480)
                     /locus_tag="ACG8OO_002370"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046218.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZKBH010000108         8273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000108 DAZKBH010000000
VERSION     DAZKBH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.109"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            296..829
                     /locus_tag="ACG8OO_002371"
     CDS             296..829
                     /locus_tag="ACG8OO_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046219.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(955..1632)
                     /gene="nth"
                     /locus_tag="ACG8OO_002372"
     CDS             complement(955..1632)
                     /gene="nth"
                     /locus_tag="ACG8OO_002372"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9046220.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1639..2337)
                     /locus_tag="ACG8OO_002373"
     CDS             complement(1639..2337)
                     /locus_tag="ACG8OO_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9046221.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2605..3789
                     /locus_tag="ACG8OO_002374"
     CDS             2605..3789
                     /locus_tag="ACG8OO_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFP9046222.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3795..4238)
                     /locus_tag="ACG8OO_002375"
     CDS             complement(3795..4238)
                     /locus_tag="ACG8OO_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9046223.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4464..4751)
                     /locus_tag="ACG8OO_002376"
     CDS             complement(4464..4751)
                     /locus_tag="ACG8OO_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9046224.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4922..6274)
                     /locus_tag="ACG8OO_002377"
     CDS             complement(4922..6274)
                     /locus_tag="ACG8OO_002377"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFP9046225.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6547..6993)
                     /locus_tag="ACG8OO_002378"
     CDS             complement(6547..6993)
                     /locus_tag="ACG8OO_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9046226.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(7229..8155)
                     /gene="cysK"
                     /locus_tag="ACG8OO_002379"
     CDS             complement(7229..8155)
                     /gene="cysK"
                     /locus_tag="ACG8OO_002379"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9046227.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2277 a         1656 c         1320 g         3020 t
ORIGIN      
        1 aaaaaagaaa ccacgtctac aagcgtggtt tcttttttgt gtctatgtaa aaaacagcta
       61 caaataaaaa tcatgtacca agctgtatta catgattttt tataagacta tccagcatat
      121 acatgtgtga tctaacattt agcttaaaaa ttagaaaatc catttttctt ttatcattac
      181 atatacttta tccatttact tgtttataat taaatcggct aaatacacac tagtctttta
      241 tttagtgcca cttcttaata atcccattgt tattatcaaa taaaaaatac aatatatggt
      301 tttgaataat tttttgaaag aaggcttcat tttgagttat ctgatagtat gcttaattat
      361 cataatttgt ctgcaccatc tttttttatc gaaaacaata cctcgttatt ccaagaataa
      421 aaaagctgaa aaattttgtt tattattaaa taaatttact ttaattgctc ctatccttgc
      481 atttattatt ttttctgtac ttttaagcac tactcttaaa ggaaaattta tggaaagaag
      541 ctctcatgca atgattctca cttttttatg gttgttgttc accagaatct atatttttct
      601 gatgagcctg aaaccgccca aatccattag tttatgttta gttataaatg gtattttttt
      661 attatcactt atcattttta ttacaccttt agatagatac gtcacttatt tatataatcc
      721 tttggaatac tggacttatt ttataggtat attggaaggc attattttct acatcgggta
      781 ttttcccaat aaaaataaca acttttattt ttatcgcaat aaattgtaat aagttaccat
      841 cctagctact gagtatgtac atttactttt tcatgcaaaa aagataaacg aaacagcttt
      901 tctccataat aacaagataa ttcatacatt tatttgcaat taaagaaacc acgtctacag
      961 gcgtggtttc ttttttagcg ccttttcttt tagacgcatc cgatttttcc catcttgaca
     1021 tattgatagc aaaggacata cctcgcactt gggatttctt gccgtgcaat gatatctacc
     1081 aaaaaaaatc agtgtatgat gtgtctttac ccataattct tgtggtactt tccgcatcag
     1141 tgtttcttct acttccatga ccgtagcatc tagtttgcag atcctcaggc gttttgagac
     1201 acgttccacg tgggtatcta ctgcgattgc tggtatcccg aatgcatccc ccaatacgac
     1261 atttgctgtc tttctgccta caccaggaag agacatcaat tcttctcttg acgttggcac
     1321 ttgcccatcg aaacgttcaa tcagctgctg tgcacacgct ttgatgtttt ttgctttatt
     1381 tcgatacaaa ccaatcgttt ttattttaag aatgatttca tcgattgaag cttctgctaa
     1441 cgcatcgggt gtcggaaaag aggcaaacaa atctggcgtt gctttattca ctgatacatc
     1501 tgtcgcctgg gcacttaaga tcactgcaat caataactca aatggattat tgtgcttgag
     1561 ttctccatgt gcttcgggaa acattccata catcgtttct agagcttcca ttgttttttg
     1621 tttgcttaac atattccgct actcctccaa ccaatttttc agtgagactt tcggcaattc
     1681 ttcttgttga gctgactgtt ctacttctct ttgaagaagc tgctgttttc ttcgtttttg
     1741 gtcttcttcc acttgttgtt ttgtacgtag attttttctt tcccacgata gcaaaatgcg
     1801 gtcaacgtaa ttaaaactat aagcctgatt taatacggct tcccgcagtg ctagcttcaa
     1861 aatttccggc tgataatggt cttcttctag ccattgtccg atccgctgga actcgatcga
     1921 agaaagcggg cgtccaaatt cttgttcaaa taattgatac actgactgga tttctttttc
     1981 ttgtgttttt tcttcgtatt ttttttcttg tgtttttaaa tactcaccta atagatcata
     2041 gatggggtaa agattatatc gatctgcttt tttaccgttg acaatcgtcg tttctatctt
     2101 gataaatcca tttgttacga gacgatccag tatttgaaaa atctgatctt gtttgattcc
     2161 catatccata gcaatcatct gcaaatcagg gaaagtatct ccttcaagct gtgccatatg
     2221 caactgtaga acaaataaaa attcttcaga ttgtagaccg atcctatgat aatttttaag
     2281 taaaagatta gatatgactg tttgtccagc gcttaagtaa ttttctaaat tcaacatgat
     2341 ttctcctccg ctcaatcaca gtatacgtaa aaaaaacatg ggaagcaaga acaggtttct
     2401 tttctattta cttcactgat cggtcagatt tacaatgtcg tctacttgat ttttgtttgt
     2461 tcatgttttt gatgcgtatc ttcacttttc ggaataaata aattaagtag atttcgctgt
     2521 tccttagaat gttgacaagg aaacaaaatt actatacact atttttgttt ccaagttaac
     2581 attattgcat aaaggagttt ttacatgtac agtttcacaa ataaacattc taaagttttt
     2641 ggatacgtct ctgtcttatt cactggatta ggatttacta tcgtcagtcc tgtacttccg
     2701 tttttaacgc tgccttattc tcactccata catcagcaag ctttctatat cactttgctc
     2761 atgtctgttt atgctctagc tgccttctta tctgccccca ttctcggtag cttaagtgat
     2821 cattttgggc gtcatccgat ccttattatc agtttattag gatcaagtat cggatatttg
     2881 ctttttggtt taggaaattc gatatggatg ttgttccttg gtcgtatcat tgaaggattg
     2941 acggcaggag aaatctccac cttatatgct tactttgccg acatcacgga acctaatgaa
     3001 agaaccaaag tttttggatg gatgggtgct ttagtcggta taggaacaac gctgggtcct
     3061 attatagggg gtctattagc tgagttgggg aattctgttc ctatatttat tggagcactc
     3121 tttactttct taaatgccgt ttatggctac acatttatgc tagaaagcct tccaataaaa
     3181 aaacgttcag tagatttaag tttctcccat gtaagacctt ttcaccaatt acaacaatta
     3241 ttcaagattt catccgttat tcctttattg acagcaggtt ttgctgtttg gttggcagct
     3301 ggctctcttc aatctatctt ttctcaattt tcgattgacg cctttcagtg gaaggctggg
     3361 ttggttggtt taagcttctc tttgattgga atcttggata ctgtctcgca attgttcatt
     3421 atgcctcgat tgctcaaaaa attttcagaa cagcagatca caagaatagg gatgttcagt
     3481 gaaatcctcg cctatctatt catcacatta tctggaattc ttctactgcc gattttgttt
     3541 ttatttggga ttatctgtta tggtttcggt gattctattt ttactcctgt attcaatggc
     3601 caactatcca attctgtctc agataaccag caaggtcttg taatgggcgg tacccaaagt
     3661 atacaatctc tatcgcgcgt catcggcccg ctaatcgcag gacagcttta tgctgcagca
     3721 ccatttcttc cgacggtatt cggatttttg cttatgattt ttgcctatta tcactacaat
     3781 aaaaaatagt agcttcattt gctacttcgt ataaattctc tgtatcgacc gataaaacga
     3841 aatactgtgt caaattcttc ttttgacata ttttcaaata cagtagcatc tcgctgtacc
     3901 catttttgat ggagttgctc gtgtctattc cttatttttt gtccgattgg cgtcaattga
     3961 aaatagattt cttttttatt ctcttcgctt tgataagtgt ttatgatccc tctagctatc
     4021 agtttttttg ttagcttact gatggctcct ctagtcatgt aactagcatt cgctaatttt
     4081 gtcacgttag gatcagggat tttcccgata tgttcaatca cttctagttc atttagagta
     4141 aatcctgcaa attctttttc catttctggt ttattacgat gaataatctc attagctaaa
     4201 tccagatatt cttctattag cagttgtctt ttatccattt ctacaccttc tttttctttc
     4261 attatattgt ttttttgttt cctattcaac aaagataaaa atattttgta ggaaaaccca
     4321 atctgaaacc aagaaaaaaa ggggttgaca aagcttgtca taaccccttt ttctgattaa
     4381 aagatagtaa agagacagtc atcggtaatt gtgatgattt tttgtcatca cttaaggatg
     4441 agcggccttt tcaaaaactg tctttaaaga tcatcatcag tttctagcac atatggctga
     4501 ccttcatatg tgtactctgt attcaatgtg ccgtcatttt catctgagta gatgcgtcta
     4561 ctgaaaggaa aacgatgaga aacattatct ttggcgtcat ttgctggcat cgtgtaatat
     4621 tcctcttgcg tttcattcaa ctgagagagt acttgatctg ccaactcatc aatattgatc
     4681 gttaatttct tcgaagcact tttataatct tctaaaaatg catcttgttg gacaaaatat
     4741 tctttattca tcttctttct cacctttctt ttcaaaatca aataaagctg cttgttcttt
     4801 tatgtaacag ctttatcttt gctgcttatt gtgacaaaaa attcgaagaa agacaactaa
     4861 ttgtactttt cacaaaaaaa gacagagggt cgtgacaaaa ttgtgtcaca accctttttc
     4921 tttattgtgc agtttcgatt tcgcctagcg ctgcttgtgc aagtaggttc aagtaattcc
     4981 atgggcggtc aaaatgtggt tggaagaaga aatctgaaag tgcgagatct tctacagtca
     5041 ttttattttg aacagctaaa gacatggtat ttgctgattg tgtaatgtcg tatttggaca
     5101 tgaactgagc acctacgata cgattcgttc ctttttcata gactagctcc atcattactt
     5161 tttcagtagt tggcatgaac tctggacgat aattatcttc aaatacagtc gcttggactt
     5221 ctagatcgtt caaagttgcg ctttctttgg tcacacctgt cgaaccgatc gtccagccaa
     5281 ataggtaaag tcctgaagtt ccttgagtac cacgatacgc cattttatga tctgtcaagt
     5341 tatttcccac aagcaaacct tgacgaacgg catttgttgc caaaggaata tagttttgcg
     5401 tgccacttgg attgtagttg actactgcag agtctccagc tgcaaaaatg tctggattac
     5461 ttgtctgcat gtattcattc actttgattg ccccgtttgg cagtgtatct acttttcctt
     5521 cgatcagttt tgtatttgga cggaagccca cacataaaat gaccatatct gtatcaaatg
     5581 tatctgttgg tgtagctact tgtttcactt ttccttcttc atctgtgata aattcagtaa
     5641 cattttcgcc tagcgcaaga gtaacaccgc ggtctgtcaa ttctttttct aagatatctg
     5701 tgaatggttt atctagatat ttattcaaga tgcgatctaa tccatcaatc aatgtcactt
     5761 gttttccaga ttcgacaaaa gcttctacta attcaatacc gatatatcca ccaccaacaa
     5821 ttactacacg ttttgcttct ttcgcttgtg caataatttc atttgcttga ttgtagtttt
     5881 tgcataggag tacatttttc gcatcgattc cactaatcgg tgggataata ggccatgaac
     5941 cagttgtcat cactaattta tcataagaaa ccgtttctgt ttctcctgtt ttcagatctt
     6001 ttgccgttac tgttttattt tctgtatcga cgtctgttac atcgtgttcc attttaactg
     6061 ttgctcctaa tgaagataat tcttcaggat ttgagtagaa caagcctgct gggtccttta
     6121 ctactccgcc gacataaagt gcgataccgc aagataagaa agaaatattg tcatttcttt
     6181 catatactgt cacttctgct tctggatgat tttttaaaat actttttaca gctgcagtac
     6241 ctgcatgagt acagccaatt actactactt tcatttatgg tccctccgat taacattttg
     6301 tccttcgaca aaactactat aacagaaata aaacggatac aacagtttaa tgcttgtgaa
     6361 ttcacaaagg tttgtttgta gtttcattcc ttttcaagca taagaaccca atatctacaa
     6421 gattcttatt ttaataatat tcacttttat atctaagatt gtgaataaaa gacaaaaaaa
     6481 taaaaaagct agcgtttttt ttacacagct agcttttttt aggtataaca cttgtagaaa
     6541 ctgtttttac atttgcgcct tttttgctga ctctttacaa tccggacaga gtccatacaa
     6601 ttccaaccga tgttcattaa tctcaaaatc agtcagtctg cttgcagcca tttctatatc
     6661 ttcaagccct gggtaatgaa aatcaacaat cttaccgcag ttttcacaaa ttacatgata
     6721 atgacgcttt gaactgaaat caaatcggct agaagcatcg ccgtacttca tttcttgaac
     6781 aaatccaatc gaagtaaata agcgaaggtt attgtaaaca gtcgcaacac tcatatttgg
     6841 aaaccgatgt tcaagtgctt tataaatctc atcagccgtt ggatgggaat gatgttcaat
     6901 caaatattct aaaaccgcgt accgttgagg tgtgattcga atgttctctt tttttaactt
     6961 ttcgataacc tcttcgacta acttattttc catgtaaaca ccccacgttg ttattctcgt
     7021 cttatcatac tatttttaca ttttaaaatc cacttaacat gaaaagaaaa ttcaatttta
     7081 ttgtatttta atgaaaaaag acgtaaaaca gtcgcgttca ctattctacg tctctattca
     7141 aataattttt ctcatcataa atttttgtgt gttcttttca tctttaaaag ttttttacat
     7201 aaacctatct ggagaaaaac caagtgcctt atatctcttg gtaaagggct gtagataaat
     7261 aacgttcacc attgtcggga accacagcta ataccttctt gccttttcct aattcttttg
     7321 cgacttttag cgcggcagca attgcagcgc cagatgagat gcctactaaa atcccttctt
     7381 ttcgcccaac ttctctagct gtttccatcg cttcgtctcc actaatagaa aggactttat
     7441 catacacaga agtatccagt gttttaggaa caaaacctgt accgattcct tggattttgt
     7501 gaggtcccgc ttctttccct tctaaaatag cagattctgc tggttctacc ccgattaatt
     7561 cgattttcgg atacacacgt tttaattctc ggccagcgcc agtgatcgtt cctcctgtac
     7621 cgatacctgc aacaaacgcg tccaagccat ttactccaaa tgcttgatgt atctcaggac
     7681 cagtcgtctt ctcatgcaca agaggatttg cttcattttc aaattgtaat ggtaaaaagt
     7741 agccgttttc ttttgccaaa cgttctgctt ctctgattga tccagaaata ccatccgcac
     7801 caggagtcaa aatcaactct gcgccgtacc ctttcatcaa taaacgtctt tcgatactca
     7861 tcgtctcagg catcacgatg attactttat atccttttgc aactcctacc atcgacaatc
     7921 cgattcctgt atttccagaa gtaggttcaa tgatagtatc acctggtttc agtagaccat
     7981 cgtgttctgc tttttctatc atacttaaag caatacgatc ttttacactt cctcctggat
     8041 taaaaaactc aagctttaca aaaacatctg ctgaatcttc tggtacgatt ttatttaatt
     8101 tgacgattgg cgttttgcca atcaattcgg tgactgagtt atagatagct gtcatcttca
     8161 attcctcctt gtctttgctc ttcttactta tcataataca atctgttcat ttatggcgag
     8221 tgattcgtct ttgactagca aaaaaaagac aaagatatac catatatggt aat
//
LOCUS       DAZKBH010000109         8187 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000109 DAZKBH010000000
VERSION     DAZKBH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.110"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..366
                     /locus_tag="ACG8OO_002380"
     CDS             142..366
                     /locus_tag="ACG8OO_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFP9046228.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            508..2259
                     /locus_tag="ACG8OO_002381"
     CDS             508..2259
                     /locus_tag="ACG8OO_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFP9046229.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            2259..4043
                     /locus_tag="ACG8OO_002382"
     CDS             2259..4043
                     /locus_tag="ACG8OO_002382"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9046230.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            4369..5220
                     /gene="licT"
                     /locus_tag="ACG8OO_002383"
     CDS             4369..5220
                     /gene="licT"
                     /locus_tag="ACG8OO_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFP9046231.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            5313..7199
                     /locus_tag="ACG8OO_002384"
                     /pseudo
     CDS             5313..7199
                     /locus_tag="ACG8OO_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(7239..8141)
                     /locus_tag="ACG8OO_002385"
     CDS             complement(7239..8141)
                     /locus_tag="ACG8OO_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9046232.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT         2566 a         1398 c         1770 g         2453 t
ORIGIN      
        1 agatgacgaa agaaattttg ttctcctcta aaaaaaccac tattttttgc atgaaactaa
       61 agaatacgcc tttctttttt caataaactt tagtaaaata agtcattgag agcaattcgg
      121 aaatagaaag gagagattta tatgttatca acaggtattg tcgtacttat tgctattatt
      181 gctttgctaa tcggcgcagt aggtggcttt ttcctagcgc gtaaatatat gaaagactat
      241 ttagaaaaaa atcctcccgt taatgaggac atgttgcgat caatgatgat gtcaatgggg
      301 caaaaaccat ctgagaagaa aattcgccag atgatgcagc aaatgaagaa ccaagggaaa
      361 aaataggttc tgacccaacg actttttacg ggctgatttt ttcagtccgt tttttatttc
      421 gtaatttgtt agactattca gattattcag caaaaaagac aagcacagac aatcctaggc
      481 tcttgataaa gacaaaggag gagtaaaatg tcgattttta gaaaattagg ctggtttttt
      541 cgtcaggaaa agaaacatta tttaattggg gttttctcgc tattcgcagt ggccttggta
      601 cagttagttc ctccaaaagt gattgggatc atcattgatg agatcgccga taaaaattta
      661 tcaataaaga tcattttcgg gtggatcttc ctattgatta tcgctgcttt tttacaatat
      721 ctttttcgtt atatctggcg gacgaatatt tggggaagtg cagctcgatt ggagaaagaa
      781 cttcggcagc agttattcaa acattttaca gaaatggaca atctctttta ccaaaaatat
      841 cggactgggg atttgatggc tcacgcaacg aatgatttga atgcgatcca gaatgtagca
      901 ggagctggta tcttgacctt tgcagattca gtgattacgg gcggaacaac gatcattgcg
      961 atgattttgt ttattgattg gcgcttaaca ctgattgcgc tgattcctct gcctttgctg
     1021 gccgtcactt cacgagtgct ggggtcaaaa ctccatgatg cttttcgtga ttcgcaagaa
     1081 gctttttcta gtatcaatga taagacacaa gaaagtatca ctgggatcaa agtcattaaa
     1141 acatttggac aagaaaaaga agatctagcg gattttgaag aaaagattga agatgcaatt
     1201 attaaaaaca aacgagtcaa ttttttagac tccttatttg atccattcat taccttgatt
     1261 atcggtctgt cttatgttgc aacaattatt ttaggtggaa agtatgtctt ggaaggcacg
     1321 atctcgatcg ggcagatggt ctcatttatt agttatatcg gtatgcttgt ttggccgatg
     1381 tttgcgattg gtcgattgtt caacgtattg gaaagaggaa atgcttctta tgatcgcgtc
     1441 aatgaacttt tgcatgaaaa gacgcatatc gtggaacaac tgggtgcagt gcagaccccg
     1501 gcaaaaggcg agctgaatgt agctatttcc tcttttaatt atccgaaaga agaatcacca
     1561 gcattagaac aaatcaagtt tgcgatccaa gaaggagaaa cattaggtat tgttgggaaa
     1621 actggtgcgg gaaaaacgac gatcatcaaa ttactgatgc gtgaatacga tgattatcaa
     1681 gggaaaatcc aatttggcgg acgttcgatc aaagaatatt cattgaatgc tttattagga
     1741 tcaatcggct atgtgcctca ggatcacttc ttgttttcga tgaccattcg agataatatc
     1801 cgtttttcta atccaaaact ttcagaagaa gaagtagagc atgcggcttc tttagcattt
     1861 atcaacaatg aaatcaagga attaccaaaa ggttatgata ccttagttgg agaacgtgga
     1921 gtctctttat ctggcggaca gaaacaaagg atttcgattg ctcgagcatt gatcaaaaat
     1981 cctgaattat taatattaga tgatgcactc tctgcagtag atgccaaaac agaagaagct
     2041 atcttgcata atttaaaaga aataagaaaa gagaaaacaa ctatcattgc agctcaccgt
     2101 ttaagtagcg tgatgcatgc aaaagaaatc attgttgtcg atcaagggaa aatcattgaa
     2161 cgaggaaccc atcatgagct gcttgcacta ggcggctggt atgaacaaat gtgggagaaa
     2221 cagcaattag aagcgaagat agaaggaggc gaggcctgat ggatgaaaat tatcaatcgg
     2281 aatggacaaa atcgattcca cttaaagaac agaaaaagat catcaaacga ctgatgaagt
     2341 tcgccaaacc ttttagaaga acgttcatcg ttgctatctt atttgcgttt gctttgtctg
     2401 tgatcaatgt cctattgcca aggatcatcc aaacttttat ggatgatcat ctggctaaac
     2461 agtcggctac gacacaagtt attttgtttt ttgctggtat gtacttattt ggagtcatag
     2521 taaaaagtat catttggttt ttccaatggt atttgtattc catggcctca ttgaagacct
     2581 atcagtatat tagggtaaaa ttatttgaaa agctgcatac actaggtatg cgttattttg
     2641 atcagacacc ggcaggatca accgtttctc gagtgacgaa tgacacagaa acgctatttg
     2701 aattttggta cgtctttttg atggtattga ctgggatctt tgcagtcgtt tcttcattcc
     2761 ttgccatgtt tcaaatcaat ggagaaattg ccttgtggac tttggtgttt ttgccgattt
     2821 taggaatcgt catctggtat taccagaaat tcagttctcg tatttaccga aatatgcggg
     2881 aaaaattaag tcagctgaac acgaaattga atgaatcgat ttccggcatg cgtattatcc
     2941 agcagtttcg ccaagaagct cgtttagcta aagaatttga acaagtgaac aatgagtatt
     3001 tagacacgcg ttatgcgatg atcaaaacga attcgattct gttaagtccg atcatcaatt
     3061 ttctatatgc attggcgatt gcgttggtgt tgactttgtt tgggatcgat gcgttgcatt
     3121 ctcccgtaga agttggactg atctatgcat ttacgaccta tgtccaggga ttttttaatc
     3181 caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc
     3241 gtatcttgaa gattatggat acggaagaat atgcaccaca acaaaaccct ggagcagatc
     3301 aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc
     3361 acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg
     3421 gacacaccgg aagcggaaaa agctcgatca tcaacgtatt gatgcggttt tacgaatttt
     3481 atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac
     3541 gtaagaaaat ggggctggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa
     3601 atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg
     3661 ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac
     3721 atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga
     3781 ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag
     3841 gattgatcca agaaggattg gcaaaaatgc gtcaaggacg gacaacgatt gcgattgccc
     3901 atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag
     3961 tagaacgcgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca
     4021 aactgcaaag cagtggtata taaaagatgt tgtgacagta gaattttgac ctaagcacta
     4081 gagtgaaaat aaaacatagc ttctcatatt tttcatctgt gagagttagt gtcgtagtca
     4141 ccagatttaa aaaaagattc gacagctagt cagaagtgcc taatagccac taagaaagcg
     4201 ttgacaaaag catttgcttt cgataatata aagaaaacga tacaacttgg atagtgattg
     4261 cctgatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt
     4321 tctttttgtt ttggatacag ccctatacat catggaaagg agcaaaacat gctgattgag
     4381 aaagtactga ataacaacgt cgtaataacc agggatgaaa gaaatcagga aatgatcgtg
     4441 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
     4501 gacaaaaaat ttcagttgac aaatgacggg ctttcacaac agttccaaga attggcacaa
     4561 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggct
     4621 ctacaagtcc agttgaatga ttctgtgtat attgcattga cggatcattt gtatacggct
     4681 atcgaacgtc acaaaatggg aatatttgta ccaaatgtat tgctgtatga tatcaaacaa
     4741 ctatttccta aagagtatgc catcgggaaa aaaacagtga agaaaatctc ggataaatac
     4801 cacatcaagt tatcagaaga tgaagccgga tttatcgcat tgcatttagt caatgcacaa
     4861 ggcattcaaa gccagcaaga caatacctac ctgtttacta taacaataca ggatattcta
     4921 aatatcgtcc gctattacta taagattaca tttgatgaag attcagtcca tttttttcga
     4981 ttcgtcacac acttacgttt ttttctgaca cgcgtcctag atggagaaaa atcagaagtg
     5041 ggagaagttt ctgaggaatt gtttcaggtt attcaaaaaa aatataagga tacgtttggt
     5101 tgcgttgaga aaatagcaga ctttttagcc gagacgttca actaccagat gtccacggac
     5161 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
     5221 aaaaaggagg ataaattaac aagaacgtat gaaataaggg tagtgacatt cagttggcta
     5281 aaccttccac taataaattg gagggaatag aaatgggaaa ataccaagaa ttagcgagaa
     5341 aaatcgtaga aaatgtcggc gggaaggaaa atatcaatgg tttgacacat tgtatcacaa
     5401 ggcttcgttt caaattaaaa aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg
     5461 acggtattgt aacggtaatg cgagcgggag ggcagtatca agttgtcatt gggaatcatg
     5521 tgccagtcgt gtttgaagag gttataaaag caggaaacct tacttttgat gaggctgttt
     5581 caacagaaaa aatgagaccg tttgatatgc ttattgatgg aatatcgggc tgtttccagc
     5641 cattccttgc tatcctagct gctggcggga tgattcgtgg actgacagca ttccttgtct
     5701 tcttaggcgc gtttgaccgc ggttctggca catttgttat gtttgataat atcggtgatt
     5761 ctgtattcca atttatgcct gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa
     5821 atgaattcgt agggatgttg cttggcgcag cattaatgaa tcctagcttg tcgctggaag
     5881 ctttgagtgg tgcagcagaa gcaccactaa cgaccatatt ttctggcact atttttgaag
     5941 cacctattta tcaaacagta ttcggcattc cctggattgc gcgtaactac gcttcttctg
     6001 tcattcccat tatctttatt gtgttgttag ctagccaaat tcagaaacca attaaaaagt
     6061 tagtacctga gatgatcgcg aatttcttcg ttccattctt caccgttctt attacaatgc
     6121 ctttaggttt tttactcgtt ggaccagtat tcacattcgc aactgatatt ttaatggctg
     6181 gatttgaaac acttttagca ctttcgccag ttatttatgg agcaatcgta ggcttcttct
     6241 ggcaaattct ggtgatgttt ggtctccatt gggcaatcgt accaatggga ttgatgcaat
     6301 tttctgtgaa tggttggcaa aatattatga cacctgttgc agtagtgtct tttggacaaa
     6361 ctgcagcact taccgcactc tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa
     6421 taccagccat tgtttctggt attgtgggga ttacagagcc agctatttat gggtttacat
     6481 taccacgtaa aaaaatattt atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata
     6541 gtgggttaat gaatttaact tcttggaatc aaggtggact aggcatcttt acaattccca
     6601 actatattag accagatggt gatttaacag atgtgattaa tgtattgatt gggatcgcca
     6661 ttgcgatggt ggtatccttt acgctgactt ttttcttttg gaaggatgaa gcaggtgaaa
     6721 cagatgatat acagaaaaaa tcaggtaaag aaattgtaaa aacaccgata caaggacaaa
     6781 tcgctccact aaatgcagca aaggatgcag cttttgcaca aggaacacta ggtcgaggaa
     6841 tcttgatcta ccctgaaaaa ggagaagtac gtgccccttt tgacggaaca gtcatgaccc
     6901 tgttcccgac gaaacaggca attggaatgg tttctgaaac agggctggaa ttgctgattc
     6961 atgtcggatt ggatactgtg cagttggaag ggaaatattt tgaaagctta gtgcagcaag
     7021 gataaagagt gaaacaaggc gatctgttgc tacgcttcga caaaaagaaa atcgaaaagg
     7081 aaggatacag tttagaaaca cctgtgatcg tcaccaatta cacggattat ttggatattt
     7141 tggaaaacca aggggagacg gtcgggccag acgatgcact tatcaccgca ctgacctgaa
     7201 cggttaggtg aagaatggac taggcttaac tattcttttt attccacttc tttattttca
     7261 aaattgaaag gctctttcat gatggctgag ctgatagggc gtttatcgaa tttttgaccg
     7321 acagaaatta gatggactaa attctgttga taattttctg caaaaacaca actatataac
     7381 acgtctagaa gataacaaat tgatgtattg atcgtaaaat taccaatttt tgaatacagt
     7441 cgctcgcgtg tcgtaagtcg caaagcggct gtactcattg cagatagcgt tgaatcaccg
     7501 atactggtca aagctaaaat tggaacatga ttttgtttca atatttctgc ggtacgaagg
     7561 atcacattgt tttcacccgt ataagaaatc agaatcccgc acatatcact cgtgcaattg
     7621 tacgcttcat agaatgattc accaaaagtg gtgacaatcg tgacatccat tttgatgcgc
     7681 ttcattttca gcatgaaatc ttgagaaatc aaagtattcg agttactggc gaaaatcacg
     7741 attttttttg cttgaagcat tagttgtttg gctttttgca gttggtcatg atgaatgaga
     7801 gaaagtgtat catgaatcgt catctgttct aaagaagcca ttttatttgc gatcgtcata
     7861 ataccgtctg tggtggaaaa aggaagattc gcatctacgt cttgaaaatt cccttcaaga
     7921 taggctgttt cagctaaaaa agcattacga aaactgaccc agccatcaaa acccattttt
     7981 tttgctacac ggatgagtgt agaggggtgg acataacatc gctcagcaat cgcttggatg
     8041 gtcatttcgc caagtgctgg agaatcgctt aagagaaact ggatcacttc attttctgaa
     8101 ggagaaaagg agatttcttt gattttttca cgtaataaca tgtagatacc ttcttcttat
     8161 ttttttacgt aaattataac ttttttg
//
LOCUS       DAZKBH010000110         7868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000110 DAZKBH010000000
VERSION     DAZKBH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.112"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..1642
                     /locus_tag="ACG8OO_002386"
     CDS             104..1642
                     /locus_tag="ACG8OO_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFP9046233.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1639..2604
                     /locus_tag="ACG8OO_002387"
     CDS             1639..2604
                     /locus_tag="ACG8OO_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9046234.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2591..3913
                     /locus_tag="ACG8OO_002388"
     CDS             2591..3913
                     /locus_tag="ACG8OO_002388"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9046235.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      3976..4080
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4154..4897
                     /locus_tag="ACG8OO_002389"
     CDS             4154..4897
                     /locus_tag="ACG8OO_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9046236.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            4912..5904
                     /locus_tag="ACG8OO_002390"
     CDS             4912..5904
                     /locus_tag="ACG8OO_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9046237.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            5916..6653
                     /locus_tag="ACG8OO_002391"
     CDS             5916..6653
                     /locus_tag="ACG8OO_002391"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9046238.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6643..7365
                     /locus_tag="ACG8OO_002392"
     CDS             6643..7365
                     /locus_tag="ACG8OO_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9046239.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7449..>7868)
                     /locus_tag="ACG8OO_002393"
     CDS             complement(7449..>7868)
                     /locus_tag="ACG8OO_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFP9046240.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2517 a         1373 c         1721 g         2257 t
ORIGIN      
        1 tttttctttt ttttgttcgt gctgtaaggt taaggcaaga caagttagtt gggaaaaagg
       61 aaagaaatgt ttatacttaa agaataacag aaaggaaggt cgcatgaata attttataca
      121 aacattaagt gaaagaaaag gcgagttgct ggaagctgca tttcaacatc tgtccatttc
      181 tctcatttct ttgctgattg cagcactgat tgctattcct ttagcgattt gggcagccaa
      241 tcataaaaaa atggcagaag tcttattgca gatcacgggt gtgctccaaa cgattccttc
      301 acttgcctta ttaggtttac ttatcccatt tgtagggatc ggaactgttc cagcattgat
      361 tgcacttgtt atttacgctt tgcttccgat tttccaaaac acgtatattg gtctagctga
      421 aattgacccg tctattgaag aagcagcggt agcatttggt atgtccagaa tgagacgctt
      481 gctgaaagta gagcttccca tcgcattacc tgtcattatc tcaggtatcc gaactgcctt
      541 agttttaatc atcggtacgg ctactttggc agcattgatc ggagcaggtg gattagggac
      601 atttattttg ttaggtattg accgaaatac acctgttttg acattgattg gtgcgatcag
      661 ttctgccttg cttgcaatta tattcagcgg actgattcgc tggttgcagc ataagaaacc
      721 tcgatattcc atcattactt tagcagcagt ggtgtgttta tttggcggcg tttctttata
      781 tcagtctgcc ttgtttaaaa atgaaaccat cacgattgct ggaaaattag gagcggaacc
      841 tgaaatcctg atccaaatgt ataaagaatt gattgaagac aacacaaaaa cagaagttga
      901 attaaaaccg aattttggca agacgagttt tttgttcaat gcactggatc aaggtcaaat
      961 cgatatctat cctgagttca caggtactgt cctagaaagc ttggtgaagg tacctgaagc
     1021 aatgaaaagt gctgatttga caaaagaaaa aacatatgac gaagcagaag aattattgaa
     1081 taagcaattc gatatgacat tattaaaacc aatggcttac caaaatacgt atgccttggc
     1141 agtcaagaga tcttttgcga acgaacatca acttaaaaag atttccgatt tggcagctat
     1201 ctctgatcag atcaaagcgg gattcacact ggaatttatt gatcgttcag atggatataa
     1261 aggaatccaa gaagcatatg gcttgaattt ttcaagtgta caaagcatgg agccaagttt
     1321 gcgttaccaa gcaatcaata atggagatgt caatattgtg gatgcctatt ccacagatag
     1381 ccagctcaag gagtacgatc tagtgactct agaagatgat cgagggttat tcccagctta
     1441 tcaaggcgca ccgctgatga aaaaagattt tgctaaagaa catcctgaaa tcgttaaagc
     1501 actgaataaa ttaagcggaa aaataacgga ggatcaaatg attgatatga attaccaagt
     1561 caatgtcgaa aagaaaaagc cgacagaagt agcacgaatc ttcctgaaaa atgaaggatt
     1621 agtaaaggag gaagatcagt gactaaatta atcgagttca aacatgtgca aaaaaagtat
     1681 gatggaaagt atgtcatcga tgacctaaat ctggcaatca aaaaaggaga aatctttgtt
     1741 ttggttggtc cttccggtag tggaaaaaca actactttga aaatgatcaa tggattatct
     1801 aaaccatcag caggagacat ctacttcaaa ggaaaatcgt taaatgaata taatctgcag
     1861 aaaatgcggt ggaatatggg atatgtgctg cagcaaatcg ctttgttccc gaccatgaca
     1921 gtcaagcaaa atattgaagt catccctgaa atgttaggtt gggaaaagca gaaaagagca
     1981 gaccgtgtag atgaacttct tcaaaaagtt ggcttatctc ctgatattta tcgagatcgt
     2041 atgcctagag agttgtctgg aggagaacag caacggatcg ggatcatccg agctattgct
     2101 gcaagcccag atgtgatttt gatggacgag ccatttagcg cattagatcc gatttcaaga
     2161 aactcattac aagaattggt tttgtcctta cacgaagaat tgggcacgac catcgttttc
     2221 gttacgcaca atatggaaga agcaatcaag ctaggtgatc gtattgcatt tatgaaggat
     2281 ggagaaatta tccaatgtga cacaccagag cagttattga tgaatcctaa aaacgactac
     2341 gttcggcatt tctttgatga accgaaacag acgaaagagt ggcgagtaga agatttagtc
     2401 gtcaatggat atttcttaaa tgaaatacca gaaaatgctc gtcaacaagt ttgctttgat
     2461 acgcctatga aagaagtata ttcgcttctt tcggtgtatc caagcattgt gatagtagaa
     2521 aagcaaagaa gtattgggtc gctgacaagt aaagaaatct ttgctttttt aagtcgagag
     2581 gaggaaggaa atgaaaacat atgatgcaat catcatcggt tcaggtgtga gcggcttaag
     2641 tgctgcttat ggattgaaag aagcaggtaa aacagtacta gtggtcgaag aagatctatg
     2701 gggcgggact tgtcctaacc gtggatgtga tcctaaaaaa gtcttgttaa gtgcagtaga
     2761 ggcacgtaat cgggtaaaac aattgagtgg aaaaggtttt aacgagatcc caactgccaa
     2821 ctgggaagaa cttcaaaaat tcaaacgaac atttacggat ccagtcccag aaagccgaaa
     2881 aaaacaatta gcagaagctg aaatcgatca tctttcagga accgctcgtt ttttggatga
     2941 ttcatctatt gaagtcaacg aagaagtctt ccatgcggat tacttagttt tggcaaccgg
     3001 gcaaagacct acgatcttac cagtggaggg aaaggaatac ttgaaaacta gtgcagactt
     3061 tttatcattg cctgtactgc ctaaagaaat catttttatc ggtggtggtt atattgcttt
     3121 tgagctagct acgattgcca atgctgcagg gagcaaagta accattgtcc atcataatca
     3181 acggccatta aaagaatttg aggcgtcttt ggtagaggaa gccgtccatc aaatggaagc
     3241 atctggcatt cagtttgctt ttggtgttga aacccaaaag atcatttcag aaggtactcg
     3301 ctatcgttta gtcggaaaag agacggaatt agtagcagat atgatttttt gtgcgactgg
     3361 gagacagccg aatacagaat ctttagcact tgagcaagcg aatatcgtct ttgataaaca
     3421 cggaattgca gtcaatgatt atctgcaaac gagcaatcca aaaatttttg cgtgtggaga
     3481 tattgtttca aggaaaaccc caaaattgac accagtcgcc acttttgaag gaaattatgt
     3541 agctaagcgg ataacggatg caacaagcga acctatcaaa tatccgatta tacctacgat
     3601 cgtctacgcc agtcctaaac ttgctgaagt aggagttaca aaaagtcacg cctcttcgtc
     3661 agatcaagtg gtagagatgg atcttaccag ttggttcacc tatcatcgtg tcaatgaacc
     3721 agttgctaag gctgaattga cctttgacca gcaaaattat ttgattggtg ctgcagtgat
     3781 tagcgagcaa gcagacgaat tgatcgatga tttgacactc gtcatcaatc aaaaattgac
     3841 taaaaaagag ttggatagct atatcatggg gtatccgaca ttggcaagtg atctctccta
     3901 tttattgaaa taatcagcat gataactaaa gcgcttgttg ctttttcctt cttgtgctac
     3961 actttaaaaa atgaatacct gtttctgggg atgtcgtaga cgacatgaga gtaagttgtg
     4021 aacttagacc cattgaacct gtcagttaat actggtgaag ggagaaacat agcagacgct
     4081 tgaagcgggt tactatgtcg tgcccatttc aagcgttttt tgttattcat attagaagaa
     4141 aggaggtaac aaaatgaaga aaaatcttta tccagcagtc agtatggcat tcattttaat
     4201 tatttggcag attgttgtca tagtttggaa aatacctgcc tttattttac ccggacctgc
     4261 tgctgtattt tctgcattag cggcagatgc acaagtatta ctttcccatt ctgtcacgac
     4321 attaaatgaa gcagtgcttg gtttggtaat agctagcgtt ctttctttta ttactgcctt
     4381 ggcaatggat tattggaagt ttttagaaat gtctttttat ccgttactag tgatttcaca
     4441 aatgttgcca atcatggtgt tagggccatt acttacccta tggtttggtt tcggtatgct
     4501 tccaaaagtg atccttgttg tcttgataag ctattttccg atcgtagttt ctttttcgga
     4561 tacattgagg aaagtatcaa aagagcaaat catctttttg aaaacgatgg gggcagatac
     4621 agggaagatt tatcgaatat ataaaatacc agcgggttta tccggcttct tttccggttt
     4681 aaaagtggct gctacttatt gtgtcagcgg agcagtcgtt ggagaatggc taagcgctca
     4741 agcaggctta gggtattaca tgatacgcgt aaaaaacagc taccagatcg ataaagtttt
     4801 tgctgcgatc atttgtgtca ttctattgag tttattgctg aatggcgctt gttcattatt
     4861 gaaaaaaggg tattatcgta tgctttatat tttttaagag aaggggatca aatgaaaaaa
     4921 ttatggcttt tattaccgct actgttgctt ttatccgctt gtggcactgc aaaagaaaca
     4981 agtagcaaac aagaaataaa ggatttgaaa aaagtgacac ttgttttgga ttatgtaccg
     5041 aatactaatc atactggcat ctacctagca aaagaaaaag gctattataa agaggctggt
     5101 ttaaatgtcc aaatcatcga accaggagat aattcgacaa gtattggtct agtaggtgcg
     5161 gacaaagcac aatttggtgt aagttatcaa gaagatgtca cttatgctca cgcagatgga
     5221 cagaatatac cagtcaaagc aattgctaca gtgatcaaac ataatacttc tggattcgcc
     5281 accctatcag acagtaacat ccattcacca aaagattttg aaggaaaaac atatgcaggt
     5341 tggcaaagtc caagtgaaga agctgtctta aaagctgtga tggaaaaaga cggcggggac
     5401 ttttcaaaac tgacaatggt aggaagcaat ggagaagggc cagaaagctt aggaaaatcc
     5461 tcagatatcc aatggtactt tgaaggttgg gatatgatca aagcaaaaga agcgggtatt
     5521 gaactaaact atatcccatt gaaagaatta gatgaacggt tagattacta tactccggta
     5581 attattacga atgatcaact gatcaaaagt gatccagaat tggtacagtc ttttatggat
     5641 gccacgaaaa aaggctatca ggaagctatt aaggatccaa atgacagtgc taaactgttg
     5701 caaaaatatg caaaagaaaa tgatcggacg tttttagaag aatcacaagc attcttgtca
     5761 aaaaattata cggatgatcc tgagaattgg ggattgatgg aagaaaaagt ttggcgcaat
     5821 tatacagctt ttatgcagga aaacggattg atcaagcaag atgtaccttc gcaagaacta
     5881 ttcactaatc agtttataaa ataggagtga tagatatgtc aatccaaatc agccacttga
     5941 atgtagtcat tgaaaacctg aaaatattag aagacatcca tttcactatc cctaaagata
     6001 cctttacatc gattgtcgcg ccaagcggag ccggtaagtc aacattgctg aaaactttaa
     6061 caggagtcca gcctatgact tctggaacga tcaaagtaga cgatcaaaaa gtaactggtc
     6121 tccatacagc atttagttat atgccgcaag aagatatgtt acttccttgg ctgagcgtct
     6181 atcaaaacgt tacgttatat caaaaaatca atcatctaac aatcaatgaa gaacaagtaa
     6241 gggattatct ggatatcttt gggttaaacg gctatgaaca ttttttgccg gaacaattat
     6301 ctggcgggat gaagcagcga acggccttgt tacgaacaat catgaatcct tcttcttact
     6361 tgcttcttga tgaaccattt ggagcgttag atgctatgac cagagggcag atgcaagact
     6421 ggatgttgaa gctgcctaaa aaagcaaagc ggactacttt acttgtgacg catgatatcg
     6481 aagaagcaat ttatttatca gacaggattc ttgtgctttc cgcccgtccg gcacatgtga
     6541 ttgctgagat tcaagtgcct gaaaaacgac ggtcacgtga atggctactg cagcaatcag
     6601 agttgaagca aacgatttat cagttattgg caggtgaatt agatgttaag tgatgctcat
     6661 tgtcatttag atgggagtca gagtttagca ctccttcagc aggaacacac gattttgacg
     6721 atcatcaatt gtgattcgcc agaagaatgg aaggaaaaca gacagcttgc agcaagtaag
     6781 actcaagcct taagttacgg tatccatcct tggaaagcag atagctatac gtttgaacaa
     6841 gttgagcctt ttttgaaaaa agcaaggatc atcggtgaaa tagggttaga taatatttgg
     6901 acgaatgtac cgatgactac acaaaaaaaa gtatttgagc gacagcttgc ttttgcagcc
     6961 ataaatgaga aacctgttgt actgcacaca aaaggatgcg aaaaagagat cttaaattat
     7021 atccaagctt accctaatca ctatttgatc cattggtatt cttctttgga ctatcaacaa
     7081 gactatattg atttaggctg ttatttcagc attggtcttg atttgaaaaa gaatccagca
     7141 gtctggcaac tagctaaagc agtcccaatc gaccacttac ttattgaaac ggatggacta
     7201 catgcagcag agtgggcttt aggggggatt ttttcagaaa aagattatgt ctcactgctt
     7261 gaagaacagt taaagacaat cgctgagatc aaagagctat cagttgaaga agttaaaaaa
     7321 aatacttatc aaaacttgga acgtttcatt agattaggta aataaaaaag agatgtgaca
     7381 caactagtgt cgcacctctt tttggctttc tttgtataaa agtaaactgt ttgattaata
     7441 aatgaatctt agtaccaacc gtttgcttcc cagaatgctt tcgccgcttc ccaagaacca
     7501 taacgtgaag taacatattg ttctgctact ctttcttggt ttgcagcaga gtagtcacca
     7561 tttaagtaag aagaatctaa ttggtaacgt ccgatgtaac gtccgttcgt tgctgtgtaa
     7621 gaaccgcttg attctttttg tgcgatccat tcttttgcag agcttgtgct tgctggagta
     7681 gcagcagttt ctgttacagc aggagcaggt tgtgtttcag caacaggcgc tgcttgtttt
     7741 tccgtttgaa ccgtttcttg tacaacaggt tgttcagctt ggactggtgc ttcttgaacg
     7801 gctgcttgag gttcagcagc tgcttgtgca gagccttctg ttgggattgt taattgttgt
     7861 cctgagta
//
LOCUS       DAZKBH010000111         7863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000111 DAZKBH010000000
VERSION     DAZKBH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.113"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..995)
                     /locus_tag="ACG8OO_002394"
     CDS             complement(21..995)
                     /locus_tag="ACG8OO_002394"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9046241.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="ACG8OO_002395"
     CDS             complement(1075..1950)
                     /locus_tag="ACG8OO_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFP9046242.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="ACG8OO_002396"
     CDS             2080..3987
                     /locus_tag="ACG8OO_002396"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFP9046243.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="ACG8OO_002397"
     CDS             complement(4528..7806)
                     /locus_tag="ACG8OO_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFP9046244.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2217 a         1662 c         1462 g         2522 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tat
//
LOCUS       DAZKBH010000112         7757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000112 DAZKBH010000000
VERSION     DAZKBH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.114"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..2434
                     /locus_tag="ACG8OO_002398"
     CDS             107..2434
                     /locus_tag="ACG8OO_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFP9046245.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2431..3345
                     /locus_tag="ACG8OO_002399"
                     /pseudo
     CDS             2431..3345
                     /locus_tag="ACG8OO_002399"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3418..3933
                     /locus_tag="ACG8OO_002400"
     CDS             3418..3933
                     /locus_tag="ACG8OO_002400"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFP9046246.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3930..4376
                     /locus_tag="ACG8OO_002401"
     CDS             3930..4376
                     /locus_tag="ACG8OO_002401"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9046247.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4526..5341
                     /locus_tag="ACG8OO_002402"
     CDS             4526..5341
                     /locus_tag="ACG8OO_002402"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9046248.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5547..6719
                     /locus_tag="ACG8OO_002403"
     CDS             5547..6719
                     /locus_tag="ACG8OO_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9046249.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6881..7588)
                     /locus_tag="ACG8OO_002404"
     CDS             complement(6881..7588)
                     /locus_tag="ACG8OO_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046250.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
BASE COUNT         2625 a         1461 c         1538 g         2133 t
ORIGIN      
        1 aaagagaaaa aaagccccaa actttctata catcaataaa ataataaaaa ttaaatttag
       61 aataacgtgt aaaggattat ttgatgattt tttattgaga ggagggatga aaatgattaa
      121 gaaaactcaa aataaaatcc aacgtatatg tcaaaaacat tttgtttcta gactcaaagc
      181 tgtatctaaa agaaaatact tacatcccat tatgatttcg atcatcttga tttttacttt
      241 cttagggagc ttatcaagta tttatgtaaa cgtttataca gaaacgtcaa attttgctaa
      301 ccaaacgaca gaggagacaa atagtccacc ctattctgaa atacagtcca tagaaagcac
      361 caatcagtct agcaacaacg agacaacctc aaccacatcc tcgactcaac tgattgatcc
      421 gacagaaaaa gaaatcacag cagcaaaagc agcaggggag aaagcttatt cagaaacagg
      481 tagaagccag acatttagta ctgttgcggc cactgcgaat ttcaacggac ataccatacc
      541 agatggatat acagtgatat atgccaaaga tggagtcatt gttgcggtta acggatcgat
      601 gagtgcggat aaaactgcgt tacaaggcgt aaccaaagca atcgctttta ttgataaccg
      661 tccagatatc cataatctgt ttattggctc cacagtaaat ggtactacta tcacattccc
      721 aaaaagtatc tatatggggg tggctaccgc tggaaatacg atcgattcag ttacacccaa
      781 tgcggataaa agttattctt ttacaccctc aacatgggga gggcttcctg ccggaggagg
      841 tatgagtact ttttcaatcg gtatacagtt agcaaatgat attccattca cggggtactc
      901 gatcaaaaat gggcagatcg ttgatggtaa gggaaatgtt tcaaattttc caacctatca
      961 agcagcaacg gttcaggtca atgcgaatcg gacaggaact ttttcttata cagtcaatgg
     1021 tggagcagca aaaatcggaa cgtacgggac tacattatct ggaatccatg tgggtgacaa
     1081 agtggttatc actccaacag cagtaacggg ctattctttt gcacaaacaa acggttcttt
     1141 cacagcccta gaaagtaacg tcgatacggt gacctataca ggcaaacaaa caccaatcac
     1201 tattaagtat caagactatt ttggacagat gattgctcct tctaaaataa tgaatcttac
     1261 ttacggaagt gctgcacaag acctgacgac taatacgcct actatcaacg gatatacctt
     1321 tacagctgtt tcagcgatag aaacaaagaa ccaatcagca atgagtgttt tagctagttt
     1381 agatacaaat ggaaatgtaa tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat
     1441 cctatattat aagacaaatg caacagtatc aatcaatgca aatggcagta agtactatga
     1501 tggtctttct gttgtaccga tcgttaaata tgcctttaat gataaaacag aatcaacatc
     1561 ccttttaaac aatgatctca gtattgatca aatcacatgg aatccaactg atttcaaagc
     1621 gatgaatgaa aagggagttg aagtaactgc gcctactgaa attggaacct atacgtcttg
     1681 gcaattgaca aaaagcggac tagctaaact agctgccaga acaaactatt tatttactat
     1741 cgtgcagaca tctgatgtct atacgattaa gcaaatcagc ggtgcggtag agctcagtgg
     1801 cagtaagaca tatgatggaa aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg
     1861 tgtcactagt tcgcttactc cagtacaagt ggcacttagc agtatggatt acacagcaga
     1921 tgcacaatca gcaaaaaata tggttaatgc tggcagctat acaattaaac taacgaatag
     1981 cggaatagac aaagtcaaag cagcaaatcc caagttgtca tttactgata tagccaacac
     2041 atcaggaact tataccatct ataaagctaa cgcagtgatt actgtcgatg acgctgcctt
     2101 taattatgat gcccaaagtc actcgatttc ggttggaaat ctccacgtta ccggtgttgt
     2161 cttaggcgaa tcattggatt ataccctaac agttaacagc cggacagata ttggatcaca
     2221 aatagtaacg attagtttag gcgaaggaag tgtaaacaat aattatacga ttatcgcaac
     2281 agataccgct aagttgacga tcaatccgac tctcttgctt ccttcgacag gtgggatagg
     2341 tctactgccc tttgtctttt taggactgat ctttattttt tctggcttct tttattttat
     2401 ccatcgtaag aaaaaagcag gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat
     2461 atatttgctg ttctcttttt tatcctactg gtctttttta taggttctcc gactgtgtca
     2521 gctagagaat cacctattac atttactttg cataatcgaa tgttcgaaag cgataagatg
     2581 ccaagtatca gtcaaaacga cggaataaat atcccggata actttcaagg agtaaaaggg
     2641 ttgaacggcg ttactttcgc tatatatgat gtctcggatg aattttacaa gctgcggtca
     2701 gaaggaagta gtgtggaaga cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa
     2761 atactggcag aaaatgtgac aaaaacagta gacgaggaag agggcattgc ttcttttagt
     2821 gcatcggaca aagatgagca aggtcgtgac gctgtttacc gatttacgga aataaagact
     2881 tcagattaag taaaagaaaa atcagcacca tttgtcgttg tgcttcctgt gacagattca
     2941 tctaatcata aattgacgac catccacttg tatccaaaga gcgaacaaaa aatacctgct
     3001 gcactcacat taacaaaaac agtagagaat aagcagacag attttgcaga tggggataaa
     3061 gtcccttatc tcatcaccgt aacaattcct gagaacatca acgagatagg aacctatacg
     3121 atcaaagata cagctgatcc tcagttatgg ctagaaccag aaacgatcga ttttttgatt
     3181 ggtggagaca agattcatac atttcacaca caaaaaataa aacatggttt tgtcttatca
     3241 gattctggaa aagatcttag ttcgtttgct ggaaaaaaat taacgatccg ttaccaaatg
     3301 acattgaaag aaaacagtga aaaaacgaca tttgataacg atgtacgttt gacgactgac
     3361 aatcaaacag tagaaacaca tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg
     3421 gatcggcaaa cgaaacaaaa actagcagat gctcaatttc tagtcaaaaa aaatgaccaa
     3481 tacttagcgc aagaaaacgg gataaatcat tgggtagcca aagatgattc aagagcaacg
     3541 atttttactt ccagtaaaga tggaacattt tctgtccatg gattgtcttt tggaagttac
     3601 gaactagtgg agattaaacc gcctaaagga tatatattga gtcaatcaac cattctcttt
     3661 gaagttgaaa aaggcagtta tgatccgctt catcctatac cattggaaat catcaacgaa
     3721 ccaaaagcaa atccgacgaa taatggaaaa acgccagttc agatgcagac aaatggtcca
     3781 tcaaatgaaa aaaatggagg aagctttcca aaaacaagcg aagagatgct tggaggtttc
     3841 tcaattttag ggttattgct agtcctttca acagggacag cttggtttta taaaaaacaa
     3901 caaaaaggaa acaataggag ggaaataaaa tgaaaaacat caaaacaaaa atatggacgt
     3961 ttttaggaac tgctatcatg ctgcttcctt tcgttctcgg cctaggaact gcagaggttt
     4021 ctgcagccgt atcacctaca ccagaaaatg ttacggttaa tttgcataaa ttgaaattca
     4081 cttcagcacc agaaaatcaa ataaacaacg gaacggaatt gacatttccg aatagcgaac
     4141 cgttgaacgg cgttgaattc aacgtatatg acatcactgc tacgtactat ccaagtaaag
     4201 acacagcagt tccagcagac gctacaccct tcgcaagtgt aacgacttct ggtgaaggat
     4261 tagctaacct gactcttcca ggtaaatctg atgggaaaga tgctgtatac gtctttgtag
     4321 aaacaccaaa gcccggtgtc gaaacgtcgc caaatatcgt gttaagctta ccctagaccc
     4381 tgacaaacct ttagacacga ttcacctata tgctaaaaat gtcatcaaag aaagcaatat
     4441 caaaatccag aaagctggga acttttcagg ggtagataag ttagcagggg cgcaattcca
     4501 gatcaaaaat tcagagaatc aattcgtgac cggttttgat gaaaacactg gtgtcgctct
     4561 ctatgatcct gctgagtcag cgttgacatt cacaactgat actgaaggat ccatcagtgt
     4621 caatggatta atagacggca attatacttt agttgaaaca aaagcaccag atggttatca
     4681 attaaccaat ccacaaaaag aaactacttt tatagtaaaa aacggaaaat tgcaacaaac
     4741 aaccggtatc atccatggcg cagatgaagc caatcttggg aatccaaacg gtgtagtcaa
     4801 tcgggaaaat acaactacgg ataacacgat tactgtagaa aatgtccaaa actacggtga
     4861 tttccacttc atcaaacaag atgtgaacac gcaagaaaaa ttagccgatg cagaattttc
     4921 tattctaaga gcaaaagatc caactgttaa attaatgaaa aaaatagcaa accttgcctc
     4981 aggagaatac ccatatgctt ggtcagatga agtcacaggt tctgactggg aagaagtgaa
     5041 attgatttct gattcagaag ggaaattttc aatcgaaggg ctgaaacaag gggcatactg
     5101 ccttcaagaa acaaaagcac cagaaggcta cgcattacct aagaatgaag ctgcgtacac
     5161 tgaatttaca gtagatgatg atccaacaac ttctgatgaa acaacgatcg ataaccaacc
     5221 taaaggaatc ttgccttcaa ctggtggtat ggggatcatc gtattcatct tggtaggtac
     5281 tgctttagtt ggaggagcag tgatctattt caaaaaacgc catcaagaaa cagaagctta
     5341 aactattgga aacaaaaatc atacgataaa taaacgatag tgattcaaat ggggagaaag
     5401 gttgtgacaa gtggtcggtc tcttggtatt cgctcaacaa acggcaaaat aggaggaact
     5461 attttgccgt ttgcttcgct aatactcagg tcaaggcgct gatttcacaa cctcttcata
     5521 tacatattag aaaataggtg gacatgatga ataaaagcag aataagaata ctggacatct
     5581 tcatggcaat cattttagtc gttgggatcg ggatcttttc ttatcctttt gtagaagatt
     5641 cactgaatga ttttcttgcc cagcaaatga tcatccatta tcaaaagcaa gcatcgaata
     5701 agaattcaga ggaaataaaa aaacagcagg aaaaaatgac aaagaaaaac caacagttgg
     5761 cagagcaaaa cgtatcacca gggataggca gtttcaacca agcagttgat gcaaaagcat
     5821 taaaagattt acctagtaat gcctttttta tggaacatat gcttggagtc atcgaaatcc
     5881 caaaaatcaa tgtcagctta cctatattcg atcagacaac ggaaatattt ttgcaaaaag
     5941 gaacgtcatt attggaagga agttcttatc cgactggcgg gaaaaacacc catgccgttc
     6001 tttctgggca tcgtggactc ccagaagcaa aattatttac agatttgcct aagttgaaaa
     6061 aaggagacca gtttttcatc cagatcaatg gaaagacatt ggcgtatcaa gtagaaaaaa
     6121 tccaagtggt cttacctgac gaagtagatt ctttgggtat ccaaaaaggc agagatctcg
     6181 tgactttgct tacctgcact ccttatatgg tcaatacaca tcgactgctt gtgacggggc
     6241 atcgtattcc ttatcaagca aaagaggcaa aaaaagcgat ccaaggaatt gatcaatgga
     6301 aaaaatggaa attttttatt tggtttatcg gtatactact tggatcaatc ggcttagttt
     6361 ggctactcat cgcttattta gattctttgg ccatcgctaa gcgaaactat cctttatctt
     6421 tttatgtaaa gaacacaaac ggacgaccaa tcgaaggaat ggtcttttct gtaaaaacgc
     6481 tcaacggcaa acactatatt actagagaaa aagttccatt tgtgaaagct tctgatgaat
     6541 acggactagt gagattttct gatttaaaag gcaggaacta tcgcttgcaa catgaggagc
     6601 tcctgcttaa aatccatgta aaacataaac attcgaaaca gttttcaatg aaattgaaaa
     6661 aaggaagata taaactgcga aaagaaaaag aggtgtacta tttgattgag aaagaatgaa
     6721 tgaagatgtc caaaaaagca gagggaacag actaaatgaa aaaagagaga agctgacgaa
     6781 atggacgtgt aaactgctat ttttggttta ctatctggaa cgccagcttc tattttttta
     6841 tgtttcagaa gaattttatt attatagtat aacttttgaa ttagtttcca taacatttgg
     6901 agcaaggggt taaaccacga gcttgagctt cgcttaaagt ggcaggggta tacgtaccat
     6961 ttccacattt atgtgtatgg tattttgaac cggtcggtgt gacatagaca gtttgttcat
     7021 tattttgctc aggagctgcg gcttgtgcct gctgagaagc ctgttcagct gctgcttgct
     7081 gtcgcgcctg ttctgcttgt tgctgtgcga ttgcttcttg cctcgcctgt tctgcttgtt
     7141 gctgtgcgat tgcttcttgt tgcgcttgtt ctgcttgttg tcgtgcaatt tcttcttgct
     7201 ttttccgttc ttcttctgct tcttttgctt tgattgtgga atcaattgca gcaagacggc
     7261 ttgtcaactc ttgtttgttt tcaggtagcg cttggatagc tgctgaagca gttgcatagt
     7321 tttcttttgt aggatttgct tcagcttgtg acaaagcaga ttccgcttct gtcacttttt
     7381 tttctagtgc agctttttcc gctttttgtt tctcttcttc ttttttcttt tcaatttgtt
     7441 ctttcgatga atctgccgta ctttcggaga tcgtggtact gctgctggaa gcctgagctg
     7501 aacttgtgct ttgagtagta gacgtagtag caccacaccc actcaacaaa cttcctgcga
     7561 ccaaaaacaa cgcaactaat tttttcacta aatttcctcc ttaagtaaaa aacaaccgtt
     7621 ttcaataaat gtccttttca attgtttact tacttgttat aataattgtg ttatgaatag
     7681 tgattgagga aacttaatct aatcccgtgt tgatacacgg gattttttgc tatagttctg
     7741 ttattaagta tgcaata
//
LOCUS       DAZKBH010000113         7592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000113 DAZKBH010000000
VERSION     DAZKBH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.115"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..790)
                     /locus_tag="ACG8OO_002405"
     CDS             complement(62..790)
                     /locus_tag="ACG8OO_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9046251.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(803..2083)
                     /locus_tag="ACG8OO_002406"
     CDS             complement(803..2083)
                     /locus_tag="ACG8OO_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9046252.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2224..2826)
                     /locus_tag="ACG8OO_002407"
     CDS             complement(2224..2826)
                     /locus_tag="ACG8OO_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9046253.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3071..3277
                     /locus_tag="ACG8OO_002408"
     CDS             3071..3277
                     /locus_tag="ACG8OO_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046254.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3589..4308
                     /locus_tag="ACG8OO_002409"
     CDS             3589..4308
                     /locus_tag="ACG8OO_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9046255.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4389..5072)
                     /locus_tag="ACG8OO_002410"
     CDS             complement(4389..5072)
                     /locus_tag="ACG8OO_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFP9046256.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5290..7476
                     /locus_tag="ACG8OO_002411"
     CDS             5290..7476
                     /locus_tag="ACG8OO_002411"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP9046257.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2721 a         1280 c         1427 g         2164 t
ORIGIN      
        1 aaaagaaccg aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt
       61 tttatgaaat caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca
      121 acgtgacttc tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg
      181 atttatgaca acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga
      241 attgtaattg ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag
      301 aaaaatattg gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga
      361 agatatcttt ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct
      421 cgacattttc ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt
      481 tgctttttaa gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta
      541 aaggatctaa ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta
      601 gttcgatatc taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa
      661 gttcttccgg atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc
      721 tatttttgat caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa
      781 tgggaatcat atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct
      841 ggttttcaat tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga
      901 taaacaagcc aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca
      961 actggtttaa cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat
     1021 tcttcttgag tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg
     1081 cattgtccaa gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt
     1141 cgattggctc aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt
     1201 ttaatgacag taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg
     1261 ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta
     1321 atccttctaa gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat
     1381 catgtttgaa ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt
     1441 gcatcctgac ttgttgctct aataactgat cagctaattt cttattagag attttccaat
     1501 aataaaccaa gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag
     1561 ctaagacatc aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa
     1621 ataatatcaa tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct
     1681 ctaacgattt ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt
     1741 gaaaatactg caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgt
     1801 cgatgcttgt taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa
     1861 ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa
     1921 ttagagcagt aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca
     1981 gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa
     2041 ttgtgtaaat caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt
     2101 gatttttacc aaattatagc acaattagcg tattattttt ttaatttttc agggattttg
     2161 aattcagaac tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat
     2221 tgtttaatga ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat
     2281 aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga
     2341 agctgagaaa aataaagtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa
     2401 taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg
     2461 aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa
     2521 ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca
     2581 aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag
     2641 aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc
     2701 tagttttcca agattattca ataaaacttc taaggcaaat tttacttgga catacaccat
     2761 atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc
     2821 tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt
     2881 tgtttcataa aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt
     2941 tttccaagaa ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca
     3001 agtttgtgct ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg
     3061 agggattctt atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc
     3121 tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat
     3181 gatacgtgtc atgaaatgcg cagtagaaga caatgatacc gctcataatc tgcactggca
     3241 gttagttgta cgcaaatcaa gtacaataga agaataaaaa cagataaaat gttgagagca
     3301 taaagaggtt gtgagtctcc cgtttagctc tgagttacaa ggaataatgt taaaaaacag
     3361 cttttcatgt tttatgaata ttgccttgtg accgaggtgt tggcaggaga atactgttta
     3421 tttgaaaaaa gggctgcgac aagacttttg tcacagcctt tattttcatg aataggagtt
     3481 acttttggct acttttagag aagtagcttt taaggagcag ataaagtcaa ggcttttatt
     3541 tctataaatg aagtagtaaa atgaagaaca gattttatta caggaaggat gccgataatt
     3601 gtgatcattg ttgcaggaac gatcggtgca gggaaaagca cactaactga aatgcttgcc
     3661 caagatttag aaacaaaacc tttttatgag aacgtggaag ataatgaagt attgccgcta
     3721 ttttattcta atccagaaaa gtatactttt ttattacaga ttttcttttt gaacaaacgc
     3781 tttttggcaa tcaaagatgc tttcagccat gatgacaacg tactagaccg ctccatctac
     3841 gaagatagta tgctgtttca tttgaatgca gatttagggc gtgtttctga agtagaagta
     3901 aagcagtatg aaggtctttt agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa
     3961 aagaaaccag atctactggt ttatattcgt gtatcctttg aaactatgtt agcgagaatc
     4021 aaaaaaagag gccgggaata tgaacagcta gaacaagatc ctgaactcta ttcctactat
     4081 aaagaactca atcgacgata cgaagagtgg tatgaacagt ttgatatatg tcccaaaata
     4141 gtgatcgatg gggacaaata tgatttcgta gccgatccgg catgtggaga acaaatcgta
     4201 caagaaatca agatgcgggc aaagaaaatg acagaagagg cagatgccca gtcgatccgc
     4261 tacactacaa ataacccgaa agcaaaggga atctttacaa ataaataata taaaaactaa
     4321 gcaaattgct cactcgcaga tatttctctg tgagcagcat cttgcttagt ttttttgatt
     4381 cattgagatc aaatagtgaa ccgattcgta tgataaaatt gccataaatg aaatgtctga
     4441 gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga
     4501 tgatgacaga aaacaaggac aatagctcct gcgataaagg caagaacgac acttagatag
     4561 tggaaaaatc gatcaaaatc tgctttttct ttagaaatac aataatcata taagttcgtt
     4621 gctacccctt ttaaatttcc agttgtaaat aaattagtgt aggcacgccc gttgatttta
     4681 tcaaaagcta cccactggat cgatgcagta aaagcaatca aaatcgttac taatgcggga
     4741 tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata atatagaaca
     4801 aacaagcgcc agaaatgttt ttcttttttt ctaaaataat caattagata ctttgttaga
     4861 agaatcccta agaaaaagaa aagggtagac aatacttttt taccaacact tagccattct
     4921 ccgtcaaatg cttggatgat cgatagaatg atattgccag tttgagcaga ggcaaaagca
     4981 ccatattgga tataagtata actgtccatg gctcctccga taaatgtcag caaaagtcct
     5041 attgtacgcc cctcgtgaaa ggaagtctgc atttttttca tcacctttta tgaatatttt
     5101 tgtcttcctt atcttagcat aaacgattga aaaaaaggaa aaattttcag aaaactaaaa
     5161 tcatatcgct tcccattttc atttttagaa aggtctgtta gtataaagaa aaaatagaca
     5221 acgaaacttt aaataaccaa cttgaaataa acaaattcaa agaagaaaga tgggaaggaa
     5281 atcaatcagt tgaatgaaaa agaaattctc cgattacaaa aagaattgaa acgctgggag
     5341 aacaagtata aaaagcttca acaagtcttt cggctacaag aagaaatcat tgctacctat
     5401 gaagcggctt atggcgaagc acccggactt attgaggaag aagtacaagt aaaagaaagt
     5461 gaaggaaaca gctctttctt taaaatgatg gagaaacgtt cctataatcg cttcaatcaa
     5521 gaacaaaaag cagctatcac atatgatatg aagaaacatc ttcgaatcat agcaggagct
     5581 gggagcggca aaacgcaaac aatctgtgca aaagctgttt atctgatgac gcaaaaacaa
     5641 gtagacgaag aacgcatact aatgatcact tttacaagga acgctgcaaa tgaactaaaa
     5701 aaacgagtag ataatttcag ccagagaaaa accgctgtcc atatcggaac attccatagt
     5761 atctttttta ggatatacaa tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga
     5821 atacaaggag acttttcaaa ggatactgca caaaaagtga atgctgtttt gcaacagctg
     5881 atacgtaaat ataatcttta tatatttgat caatatggag aaaaaacgat tgcttctcgg
     5941 ttagactatt ggcagaatat gaacctttca gtagaagaaa tggttcagtt agttaaagaa
     6001 aaatatgatt ccattgataa gaatgcacga cagccgatat cagaacgact gtatggattg
     6061 ctcacggaac tccaagaaca aaaaagaagc cagcaattgc tagagttcaa tgatgtactc
     6121 caaaatctga aaagcgcttt ggagaatgag gagatccagc attatatcgg ccaaaaatat
     6181 gattatctat tcattgatga atttcaagat actaatccac ttcaatggga aatcgtgaaa
     6241 cggatgacaa aggaaaacag gatcaagttg attgttgttg gagatgatga tcagagtatc
     6301 tatggattca gaggatcaga gccagcatac atcaagaact ttgaaaaaga atatcctacc
     6361 aaaactcttt tcttattgac taattatcgt tctcgggcag ctattgtcca aggagcaaat
     6421 agattgatca gctataataa aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa
     6481 gaagcaggtg ttatggaagc ctatttgttt tctgatacta aagcagaaag tcagtggcta
     6541 atcaaacaaa ttcagacatt tattcaaaaa aatgggaaat ataaggaatc aatcatatta
     6601 taccgatcag cttcacaaac gcagcagctt gtccaatctc tgctgaaaac gaatattcct
     6661 tttgtactgg aagcagatag tccatatgaa ggtatctttg gtatcaagtc tttccaacat
     6721 ttctatcaaa aaattgttca ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc
     6781 taccagcaat tgctccggca gctgatggca gactgctacc tgaaaaaaag tgaatgcgat
     6841 caatactttt ctcctaaata tcaaaatata gccgttgcag attatatttt gtcgataagc
     6901 cccaatttaa aatcgaaatc tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca
     6961 gaccagcatc aagaaatcta ttcgcttgtt caaagctatc ttcatctgcc acgtatagct
     7021 aaggaaatcg accagagtga tcaagaatgg atacgagaag aagtaaagca gcatgctact
     7081 ttcagaagac tccaagcctt atcgcaagaa acccaagcga cagctaaaga attaaaaacc
     7141 cgcttgattg cttacagaca aggaaaactt gatgcactat gtatccagag cattcacaaa
     7201 agtaaaggtc ttagttaccg aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct
     7261 tataacggag cgacagaaaa gaaagtaatc gactttaatg aaataaaagc agaaccagtg
     7321 acgacgatag aagaagaacg aagactattt tatgttgcga tgactagagc aagaaatcgt
     7381 ctgtatctat gtgtacctca aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc
     7441 aaagaaaccg ggagtcgtgt gaaaaaagga aagtgataga agagagggtg tgacagaagt
     7501 ggtcagtttc aagaaagaag aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat
     7561 ttcagcttat tttcgaagaa gctatttctg ga
//
LOCUS       DAZKBH010000114         7564 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000114 DAZKBH010000000
VERSION     DAZKBH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.116"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..391)
                     /locus_tag="ACG8OO_002412"
     CDS             complement(101..391)
                     /locus_tag="ACG8OO_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046258.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(509..769)
                     /locus_tag="ACG8OO_002413"
     CDS             complement(509..769)
                     /locus_tag="ACG8OO_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046259.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(906..1319)
                     /locus_tag="ACG8OO_002414"
     CDS             complement(906..1319)
                     /locus_tag="ACG8OO_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFP9046260.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1775..2260)
                     /locus_tag="ACG8OO_002415"
     CDS             complement(1775..2260)
                     /locus_tag="ACG8OO_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046261.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2399..2614)
                     /locus_tag="ACG8OO_002416"
     CDS             complement(2399..2614)
                     /locus_tag="ACG8OO_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFP9046262.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2838..3638
                     /locus_tag="ACG8OO_002417"
     CDS             2838..3638
                     /locus_tag="ACG8OO_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9046263.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3657..5525
                     /locus_tag="ACG8OO_002418"
     CDS             3657..5525
                     /locus_tag="ACG8OO_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP9046264.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5518..6009
                     /locus_tag="ACG8OO_002419"
     CDS             5518..6009
                     /locus_tag="ACG8OO_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP9046265.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5997..6551
                     /locus_tag="ACG8OO_002420"
     CDS             5997..6551
                     /locus_tag="ACG8OO_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP9046266.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6569..7564
                     /locus_tag="ACG8OO_002421"
     CDS             6569..7564
                     /locus_tag="ACG8OO_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP9046267.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2692 a         1211 c         1436 g         2225 t
ORIGIN      
        1 gattgatttg agatcttttt atacttcaaa aaagataaca cccaactttc aaaaatatgt
       61 tttggaagtt gggacaaacg agttaataca ttttaataca ttatttttta ttcaaaaaaa
      121 atagcacaat ggcaaagatc gtaagagaga tcataagccc caatgaatga ctaaaaagaa
      181 aagcagaatc acatgccata tcatcccata tttcttcaag aaaataaata agcgggaaaa
      241 agggaaggaa tagtagaaca tcgatccagc accatttttt tcttcttttg aaataacata
      301 aaacaataaa catgaacgtg cagggaagta aaaacaaagc tttactcata actaacaaaa
      361 taccagctcc ttttattttt ttctccccca cattcaaaga gtaacaatgg tatgtactta
      421 ataatagaaa aatggatcaa cttgtatgaa taacagctga aaagatctgc accaaataaa
      481 aaacttccag ccaaaactga ctggaagttt atttttttct gaaatattct aacacaaata
      541 tgaaaaagat ggccgaagct aataagccga tttcgtagca aaacaatgag ataccagagt
      601 cgtaataata aaatcctcta atcttatgga taaaatagat catcggacaa gcagataaga
      661 gtaagtattt gttaggcctt ttgaataagt aaaagaaagc actggtcatt atgataaatg
      721 gtatccaaaa caaaagttta cttaagacaa tcaaatgatc aactcccata ttttttacca
      781 agagagatca taacatcttt atcgtaaaaa caaaatattt ttttgctcat tttgaaaatt
      841 taaacatact cgttcttatc caaatatata aaaatcgccc ctcattcatc gagaggcgat
      901 aagttctaat ttaactcaat tttttttgtt tcttttggct gataagctgt tttaggtaat
      961 accaatttca gcacaccgtt tttaaaggtt ccagtgattt tttcttcatc aacattagga
     1021 agatagaatg aacgtttgta agagaccgaa tgtctttctt tccgtacata gttgccatct
     1081 tctttatctt ctgtatggga ctcatgattg gcggagatcg tcaaagtatt atctgaatat
     1141 tcaactgtcg tgtcttcttt gtcacagcca ggcatatcag cagttagttt gtattctttt
     1201 tcattttcga ctaaatctac ttttggataa cttgaaacac ctaaaaaatc attgaatgct
     1261 ggactaaaca cgtcattgaa atcagggaac atgtcgcgaa tgctaggtac atttgccatt
     1321 tttgatcatc ctttcaataa gtatttttta tttatcccta tttacgtaat aactctattt
     1381 tgaaaaaaat gcaagtataa tggttagctt tgtgaatatt tcatctaata cgatacaaag
     1441 gctatctttc taatttgaaa aagggatcat taaaataata agaagaaaat atgtcaaaag
     1501 gtaaaaaagt aaattcttct atccaaatat agaaaaattg aatctagaaa accgcccgac
     1561 aataaatttc attaactcag ttgaccgaaa atgaagtaga taccaaaaat ttatcaagag
     1621 agcaagtaga gaagtgagta tattcataag agaaatagct gtaacgaatt attaggttaa
     1681 tgcaaatgct tatttatact ccgtataact ttctcagaaa aaatgatacc aataatgact
     1741 tgctagtact gtaaatccgt ataatatcat ctaatcaaaa tggtgttcca tttgcatctg
     1801 tcatcgtaat cgatccttcg ggtgacactc gataaaaacc aacgcttcca gatcctcctt
     1861 gggaacggat ttgtttgctg gaagctgtga acaaataatc tcgatcaatt ttttgataaa
     1921 agctaataac aatatcgtca ctttgtaatt cggaacggtg ggcaataaga aatagaagtg
     1981 ctttgtcaga gttttctaat ggaagagaag gtacagtgct ttcttgcatt tcagatgttg
     2041 aagaaatgct aaacgactga atacttgatt ggctatgttg cgaatctaca ttaccagtag
     2101 aagtattagt aatggaagta gtagaagtac ttttagttga accaacctca gttgttgaaa
     2161 tggctgctga agattcttta acagaattct ttgttttata tccacaggca gaaaaaagta
     2221 ttacagttcc caacaagcct gcgtaaatcc ctttatgcat gatagctcct cctaaaatag
     2281 attatcttta ttcagtatat cacttaaaca aagaaaagca gatggtttaa aatgaaaaaa
     2341 ccttcccaat aaagggaagg ttcatgaaga taataacttt tttaataatt atagctttct
     2401 aatatctttc atcaactttt ctagtaatca ttgggaaaaa tttcaagcag tagatcgcac
     2461 aaatacctac gacgatataa atgatcttcg caaaaatagt tgttgaacca ccagcaatca
     2521 tagctactaa atcaagttcg aacaagccta ccaacagcca gttcaaccct cccacaatca
     2581 ataaagttaa tgcaatagca tctaaagttt tcattagaat tctcctttct taaatgataa
     2641 aacaattata tatcaaaaag agaaacattg cttatataac gctcaggaat tttagcaatc
     2701 tatgcaaaaa gatgaaaatt ttttttgctc ttccattagt acacattttt tagctgaagc
     2761 tgtaaatttc atgtcttaat tttttttagt gagcttttat actggatttg taagaaaaaa
     2821 gaaggagagg atcacatatg tcggattggt tggatattgc aggaaaagta gtaattgtaa
     2881 ctggaggttc ctcaggaatt ggaagaagta tcgtcgaaaa tttgttgaag cagaatgcac
     2941 aagtagcgaa ttttgatgta actgaatgcc gtatacagca tgaaaaccta ctatctttaa
     3001 aagtcgatgt gtcttctaaa acggacataa aagaagggat atataaagta atgaaacatt
     3061 ttaagacgat cgatggtcta gtcaataatg caggaataaa tattccttct cttctaatag
     3121 atagaaatca tccaaaaagc aagtatgaat tatcagaaca agtttttgat aagatgatcg
     3181 ctgtcaatca aaaaagtgta tatttgatga gccaagcagt tggaagaatc ttggtgcaaa
     3241 agggatcagg agtgatcgtg aatctttctt ctgaatcagg attggaagga tcagaaggac
     3301 agagctgtta tgctgctaca aaagcagcga tgaacagttt tactcgttct tgggcaaaag
     3361 agttaggaaa gaggaatgtt cgtgtggtag gtgttgcacc agggatactg gaagaaaccg
     3421 gtttgagaac acaagaatat gaagaagctc tttcttatac gagaggtata agcgtcgaac
     3481 agctgagaaa cggttacagt cgaacaagta cgatccctct aggaaggagt ggtaagctgc
     3541 aagaagtggc ggatctagtc tgctattatc tttcagaaag atcaagttat atcactggag
     3601 tcacgactaa tatttcagga ggtaaaacaa gaggataaag gatagggggg acgcacgtga
     3661 cgctagtaaa cagatggtac caattattga aattactagt cactcacaag aaagtttcaa
     3721 tccaggaagt gcaaaaagaa atcaaaacca gtaatcaaac cataaaaaaa gatattgggg
     3781 agttgaatga agaaattgcc ggtattgcaa aaatcatcca aaaaaacaaa cattttgaac
     3841 ttgaaattta tgattttgat ttttttgatg agatcatgca aggaaaattg aaaacagcca
     3901 gtgatttcaa ttcggcatcc aaaagaatcg cttatttact aaataggctg atcgaaacag
     3961 aggatttctt acgtatggat gatttgtctg aagaagtcgg tgtcagcaga ggaacagtgg
     4021 tcaaggatct aaagacatta aaagagatga tcaatgattt tgatgtaaag attacaggca
     4081 caccaaataa aggactaaga ataataggag atgagctaga attacgacta cttttatttt
     4141 attttgtttc tccctacttt tcagagacga aaacagaaca gtttctagaa gaaaaacaag
     4201 caatcaaaca ttttcagaag aagacgaatg ctagtaaaca agaaatcacc cttttaacga
     4261 aagttatcgg tatttctttg aatagaatct ctagtaacta tctgttgaag aagcccatca
     4321 atcgttacag tgggtgtttt gaatatatcc aagaattcaa tgaactgatc aatcagttgg
     4381 aagaaatcta tgaactaact ttgagccaat atgaaaaaga ttttatcagt tttcctttga
     4441 acatcatcaa cacaagtata acgatgataa ggaaagctaa ctctgatttg cgcttatatt
     4501 tcgaccccat gatgatgcgt attcgacgat tagatctcat tgatctggat gagtccttct
     4561 tatatcaaga gatgaaacag cacctctctt tattgataaa tcgggtaatt tttcgttatc
     4621 gattgactga tttattttat ggtgagatag aaaaaaagta tcctttttct taccaaatcg
     4681 ctagcgaatg catacaagca ttagaagcta tcttgcaaca taagatctct aaagtagaga
     4741 tcagctacct aacgttgtat ttggagttag catttcgata tcaaaaagag catttttcct
     4801 ctaaaaaagt agccattgtt catacgacag gaaaaggaac agcattaatg atccaaagac
     4861 aaatcaaaag gatattgggg agggatatac aaataacaca tgtttcagaa gaacaatatg
     4921 aaaaagtaga tctaaacaaa tatttcgctg tttttacaac gattccgtta aaaaatgtaa
     4981 atgaagagac accggtgatc catttgacta atctttttga tgactcttgg ttaagggcac
     5041 aatggcaaaa agcaaaaaaa aagcgattag ccgggtttca ttctactgtt tttttctatc
     5101 aaatatggaa agaaaaaaag acgtatcaag aatatttaaa caaattagtc gctcctttga
     5161 tcagaggcta ttatgtggat gaagaatttg aaaaaaggat cttgaaaaga gaatatcagc
     5221 aaagcacatt tgttaacaaa gaatttgctt ttcctcacac aaccaaccag ctatctcaac
     5281 acattgtttt atcagtaggg ctaataactg atcaaggttt agcagaaaca ggaggaaatg
     5341 gagttatttt tttatttgcc atacctgaag aaatgacggc tgaagttgag aatgaattat
     5401 ttgagctata cgatatccta tttgaactca taaaaatggg aatagatgag tttattgaat
     5461 tagcaccgac acaagaatca tttttaaaac taatcaaaag taaaggagag agacttcatg
     5521 aatgatatgt atctattcat agttttcgca gcttcagctt atgtgctgca agcagttatc
     5581 agttggaagc aactaaagca tttcaatcaa acatatagcg aactgcgaag aaaaggaaaa
     5641 gtagcgattg gtaaacgacc tgggaaaatc cgttcaggca cgattgttat gctagctata
     5701 gacgatcatg atcaagtttt agaagtcaga aaaatgcaag gggttacatc tttggcaaaa
     5761 tttcattcat tacctgtcta tgcgggtgaa gatatccatt acatcgatcg ttaccatccc
     5821 tcagttcgag cggaagatcg tttgacacaa gcagctttag aaaatgccag agaagtatac
     5881 ttacgaatag aggcaggaaa tgaaaaaagt gaacaacctt cattgccttt tattgctatg
     5941 ctcaataatt ttcggatatg gaaaagtacg atcaaagaaa aaaggagtga caaacgatgg
     6001 atatcataac aaaatttgct gagggattta tgaggctgtt ccaaacaggt gcagaaacat
     6061 tcataagctg gatgacagga atcgtaccag ttgtcttgat gctgatggtt gcaatgaata
     6121 cgctgattgc ttttttaggt gaagaccgag taacgaaagt agccaaatta tcagcgaaaa
     6181 atccgcttac tcgttacctt gtgttaccgt tcttatctgc ttttatgttg ggaaatccta
     6241 tgtcatttac aatggctcgt ttccttccag aatactacaa accaagctat tatgctgctc
     6301 aagctcaatt ttgtcatacg agcaatggtg tattccccca tattaatcct ggagaattgt
     6361 ttgtatggat ggggattgct caagggatcg aaaccttagg gctaaattcg atggagctgg
     6421 ctatccgata cttgcttgtg ggactgctga tgaactttat tggtggttgg ataactgatt
     6481 ttactactgg atttgtatgc agacagcaag gaatcgttct tagcaaaaaa gtagagcttt
     6541 cagttgatta gagaaaggag agttttaaat gtatggtgat attaagatcg taagaggaaa
     6601 cggcgggtat ggaggtcctt tgattattag gccgacaaaa gataaacaca agttcatcta
     6661 tgtcacaggt ggaggtgaga ggccaatgat cgtagataaa atagaagaac tgacaggtat
     6721 ggagtctatc aatgggttca aaacatcaat acctgatagt gaaatcgcgc ttgcaatcgt
     6781 tgactgtgga gggactctta gatgtggaat ttatcctaaa aaagggatac caaccatcaa
     6841 tatcgttccc acaggaaaaa gtggcccatt agcacaatat atcactgaag atatttatgt
     6901 ttcagcagta gggttagaac aaatacagcc actaatggaa actgatgata acaaagaaac
     6961 agtaaatact gcatctccag ataaaaagta caaatatgat acttctaaaa agataaccga
     7021 gcaaaaagca gagcagcaaa actggatagc aaaaattgga atgggcgcgg gaaaagtagt
     7081 tgctacattc aatcaatcag caagagaagc agtagatacg atgatccgga cagtgatccc
     7141 ttttatggct ttcgtttccc ttttgattgg tgtgatacaa ggttctggga tcggtaatgt
     7201 atttgctaac attatggcac ctttagcagg aaatgcagtt ggattaacaa tcattggctt
     7261 tatttgttca ctgccatttt tatctccttt gcttggtcca ggggcagtta ttagccaagt
     7321 gattggaaca ttgatcggtg tagagattgg gaaaggaaat attcctcccc aattagcatt
     7381 accggcacta tttgctatca atactcaaaa tgcatgtgat tttattccag tcggtctagg
     7441 tttggaagaa gcaaaagctg agacagtaga agtaggtgta ccatcagttc tttattcaag
     7501 atttcttaac ggcatcccac gagttatcat tgcttgggta gcaagttttg gattatatag
     7561 ctga
//
LOCUS       DAZKBH010000115         7412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000115 DAZKBH010000000
VERSION     DAZKBH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.117"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(185..1633)
                     /gene="cls"
                     /locus_tag="ACG8OO_002422"
     CDS             complement(185..1633)
                     /gene="cls"
                     /locus_tag="ACG8OO_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9046268.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1785..2669)
                     /gene="ftsX"
                     /locus_tag="ACG8OO_002423"
     CDS             complement(1785..2669)
                     /gene="ftsX"
                     /locus_tag="ACG8OO_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9046269.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2662..3348)
                     /gene="ftsE"
                     /locus_tag="ACG8OO_002424"
     CDS             complement(2662..3348)
                     /gene="ftsE"
                     /locus_tag="ACG8OO_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9046270.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3453..4554)
                     /gene="prfB"
                     /locus_tag="ACG8OO_002425"
     CDS             complement(join(3453..4481,4483..4554))
                     /gene="prfB"
                     /locus_tag="ACG8OO_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9046271.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4680..7214)
                     /gene="secA"
                     /locus_tag="ACG8OO_002426"
     CDS             complement(4680..7214)
                     /gene="secA"
                     /locus_tag="ACG8OO_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9046272.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1987 a         1645 c         1291 g         2489 t
ORIGIN      
        1 gtgtctcttt taatagcata tattagctta tttcttgctt tccccaaaaa acaagagata
       61 ccccaaagtt aatttcgctc atgcgagacg ctttctccat ttctgacgta ctctcagaaa
      121 tggccccgtt agaaaaggac acgagatctc catctgttga tggttctcat gtccttttta
      181 tgttttaaag gagcggcgaa atcaatcttg ctaatccttc tttcatcttg atgattattc
      241 ctcggttttc atacatttct tttgtgagat gcgtagaaat tttcgagtct tcaaaaaaag
      301 cttttcttac acgtgaagcc attcgttcat cataaaccag tgtattgacc tcaaagtcca
      361 agcgaaatga acgaacatcg atatttgctg agccaactga aacaatgcca ccatcgataa
      421 tcatcgtctt tgcatgaata aatccattct catatgtttc gatccttgct ccatactcga
      481 ttagctctgc agcgaaagaa tacgttgccc agtaaacgag aagatgatct ggtttgttag
      541 gtatttggat atgaactttt acgcctgaaa gaagagccaa cttcagtgct tcatgaatcg
      601 atccatccgg tatgtaataa ggcgtttgga tcaatatctc ccgtttggct aacgaaatca
      661 ttttcaaata agtcattttg atttgttcat gttcggtatc tggtccgctt gtcacgattt
      721 gaacagctat atcaccagta gagtgaatgg acggaaaaaa tttgggagaa tagcctactt
      781 cttttgtatg ctgagaattc cagtccatca aaaaacggtt ttgcaaggta tacaccgctt
      841 ctccgtaaat acgcaaatga ttgtctcgcc aataaccaaa tttctttacc aatcctagat
      901 attcatttcc aacattgaac ccacctgtat atccgatttt cccatcaata actacgattt
      961 tacgatgatt gcgataattg atccgaggat taatatacgg aacaaataga ggaaagaaaa
     1021 atgcgacttc tccgcctgct tttttcaaat tctgaaaaaa gctggaagaa acttgtgtgg
     1081 aaccccatgc atcaagaagg actctgactt ttacccctcg tcttgccgcc tctgtcaatg
     1141 catctcgaac ttcttcacct aatgcatcac tgcgataaat ataatactgg aagtggatat
     1201 ggtcttctgc ctgcttgata tcttcaatca gcgcatcgaa tttttcacga ccatctgtaa
     1261 ataaagtgat ttcattttta gtcgtataga gcgtactttc aaataccgtg agcatataga
     1321 tcaattgttt cacatctact tgtccagtag gcggatgagg ataaagccct cgcatcaatg
     1381 cctgtttgtc ttcttcaatt tccaggttct taccgatttt cccttggatc ttcaaatcaa
     1441 agattttctc tttggaaatc cctcttccta aaaaaatata taaaataaat cctacaactg
     1501 gaatgaacat caaaacgagc acccatgccc atgtttgagc agtttccttt ctttctcgaa
     1561 aaacgatgat aaaggaaagc aaaatattca taatcaaaag gatcgtaaaa aaattgtcaa
     1621 cgataatttt cacggcttca cctcttcttc taccaactta agaataccca agttgttttt
     1681 taaagtaaag ggaaaaaagc cgcttccttc tattctccta gccaataaaa aaagaaagct
     1741 ggaacgaaac tgtccaactt tctcatttta tttatattgt ggtattagaa tttcaagaaa
     1801 cgtctcatag aaatgatcga gccaagtgaa ccaatgatca caccaatgcc gaccattaaa
     1861 agattgattt tccaaacgat attttgtgga tgaatcaatg aataatggga acgtaataat
     1921 gatggattga tcaattggaa aaaccatgca taaccaaatg tgatgatcaa aatcggaaga
     1981 atcgcaccga tcagaccgat ccagccacct tcaaggaaaa acggccagcg aatatatcca
     2041 ttttttgcac cgactaaacg catgatttga atttcacgct gtctagacaa aatcgtgatc
     2101 cgaatcgtgt tcgaaatgag aaacatcgct acgaacagca gcaatgccgc tgcaactaat
     2161 ccccatgtcc gaactgcacc agcgatttgg aaaatccgat ctgaggaagc tccaccgtaa
     2221 tcagaacgag aaacattctt caaatctgct gcttcttctg ctacttgttt tgtataagaa
     2281 ggatcagtag cacttactac atacacatca tataacgggt tattgtcacc ttcaaacaat
     2341 ttccaggaat cacccatttc gttttggatt tttttcaatt cctgttcttt gcttgaaaat
     2401 tcaacttttt taacatgatc taagtcattc aattcttttt ctagcttttt catctcttcg
     2461 tcttttgtac cgatatctac gaatacagag acatcaacgt ttccttctat atcttgagct
     2521 aatttagttg cgttcatgat aactgccatg aatacaccga ctaaagtcaa tgtgatcgtt
     2581 accgcactga ctgaagcaac tgtcatccag ccgttacgtt tcaaactctt gatactttct
     2641 aataaatggc ggaaaaaggt tctaatcatc gtatccatat tccccttccg cttgatctcg
     2701 aataatgcgg ccgttttcga tagcaattac tcggtgccga atcgtattta cgatcgtact
     2761 gttatgtgta gccatcacga cagtagttcc ttgggcattg atccgatcta ataatttcat
     2821 gatttcccat gagttttctg gatccagatt tcctgtcggt tcatccgcga taagtacttt
     2881 aggcgtattc actattgccc gagcaataga gacacgttgc tgttccccac cggataattc
     2941 gctaggaaag actcgaactt tgtgtttcaa accaacaagg tccagcactt ccattactcg
     3001 ttttttgata tcccgaggtt ttctgccgat aacttgcata gcgtacgcaa cattttcata
     3061 tactgttttt cttggtaata atttatagtc ttggaacacc acgccgattt ctctgcgtag
     3121 ataaggaact tcgcggtttt tgattgctat tagatcatga cctgcgacgt tcaaagttcc
     3181 ttttgaagct ttttcttctc gatacatcaa tttgatgaat gtagatttac cggctccaga
     3241 cggaccaacc acatagacaa actctccttg ttcgatgaca attgagagat tgcggatagc
     3301 ggtcgtacca tttgagtact tcttcattac atccttcatt tcaatcatgg cgttctctcc
     3361 atatcttttt cagtctttag ctattatagc atggcaatgt ttcaacatgc ttgcaaaatc
     3421 ttacaatttt atttcaattt aacaatgggt tcttattgta gatgcatttt taaatagccg
     3481 tcaataaata aatcaaggtc accgtccata actgcttgga catttcctgt ctctacgttt
     3541 gtccgatggt cttttaccat agaatatgga tggaaaacat aagaacggat ctgtgaaccc
     3601 cagccgattt ctttttgttc tcctcgaagt gcagctgctt cttgttcttt cttttccact
     3661 tctaattgat aaagcttagc ttttaacatc cccatcgctt gctcacggtt tttcaattgt
     3721 gaacgttgtg cctgactagc tactactgtt cctgtaggca agtgagtgat ccgtacagca
     3781 gattctgttt tattgatgtg ctgaccacct gcaccacttg ctcggtacgt atcgattttc
     3841 aaatcatctg gattgatttc tatatcgatc gtttcatcaa gctctggcat cacatctacc
     3901 gaacaaaaag aagtatgacg tcgtttcgct gagtcaaaag gcgagatacg tacaaggcga
     3961 tgaacacctt tttctgattt cagataacca aatgcgttgt gtcctttgat cagtagggtc
     4021 acactcttga ttcctgcttc atcacctgct tgataatcta gtgtctctac ttgataccca
     4081 tgctgctctg cccagcgtgt atacatacgt aaaagcatac tgccccaatc ttgggactcc
     4141 gttccgccag cccctggatg caattcaacg atggcgttgt ttcgatcata aggaccatct
     4201 aacaacatag ataattcata tgtcttcatt ttttcatcta gcgcattgat acgtgtttct
     4261 gcttcttctt gcatggctgc atcaggttct tcctgcaaca tctccaccat caattcgatt
     4321 tcttccagtt cttcagcaag agaatggaat tgatcataag tttctttatt tgcattgttt
     4381 tcactgatca ctttttgagc agcttcgcta tcatcccaaa aaccaggttc agccattttt
     4441 gcttctgctt ccgcaatgtc ttcttccagc tggtctaagt caaagagacc tcctgaagct
     4501 tgtgaccttt tcctgcattt ctgtcaggag atttcgaatt tcggatattt ccatgttttt
     4561 tccttctttc actatacttt tttcattata tctagtctaa cagaatcagc aaataacaga
     4621 aagaaggtga gacgaattct taaaaaaaga atttgtctca ccaatcctgc tgtcagaaat
     4681 tagtttgcat tttttccatg acagttctta tattttttcc cgcttccaca aggacaagga
     4741 tcatttcttc cgaccttgtt cacatggatt ggctgttttt tcgcgctagt attactattt
     4801 tctttggcat ctccgtcttc tgagtgagaa gctgttcctt gagaaacctg ttcacgctgt
     4861 acgttttgac ggatctcagc tttcataaag attcgagtga cttcatactc gatggcgcct
     4921 accatatctt caaacatctt atagccttct gtttgatatt cgactaatgg attgttctgt
     4981 ccataagcac gtaagccgat tgactgacgt aattgatcca ttgcatcaat atggtctgtc
     5041 catttagagt ccacaacacg caagatcacg actttttcaa attccaacag ttgctctgga
     5101 ccatttagtt gagccgcttt ggtattaaat acttcttgtg ctcgttcgtt tagatactct
     5161 ttgatctcct gagctgtttt tccttcgata tctgcaagag taatactatc ttcatggaca
     5221 agagtactgc cggcaaaatc gatgatactt tccaaattcc aattttcttt ctctaattgt
     5281 gtatgcgcat ctactacacg actgattgtc cgtttgacca tgttcattag tggcttggat
     5341 aagctttttt cttccatgat tacttcttgt cgctgtttat agattacttc acgttgttca
     5401 cgcatcacat catcgtattg taagacgttt ttacgtgtgt catagttatt tccttctaca
     5461 cgtttttgtg cagattctac ttgtttcgtc agcatcttgc tttggataac agcatcttcc
     5521 tcaccgatct tcatccgatc taagaaagat ttgattcgtt ctgaaccgaa tcgtttcatc
     5581 aaatcatctt ccaatgacaa gtagaactgt gacataccag ggtctccttg acgtcctgca
     5641 cgtcctcgaa gctggttatc gatacgacga gattcatgtc gttctgtccc gataactgct
     5701 agaccaccaa gttctcttac cccaagtcct aacttgatat ctgttccccg accagccatg
     5761 ttggtagcaa tcgtcacagc acctttttga ccagcattca tgatgatctc tgcttcttta
     5821 aagtgatttt tcgcattcag cacttcatga ggaatacgtt cttgattcag catatctgat
     5881 aataactcag atgtttcaac cgcaacagtt ccgactaaca caggttgacc tttgcggtag
     5941 cgatctttga tatcttgcac gacagcgtgg aacttgcttt gtaatgttgg gtaaagcaaa
     6001 tctggacgat catcacggat aaccggacga tttgtcggaa tctggtatac ttggatgtta
     6061 taaatttctc tgaattcttc ttcttctgtt ttagctgtac cagtcattcc cgcaagtttc
     6121 ttgtacatac ggaaatagtt ctggaaagtg atcgtcgcca tggttttcgt ttcgtcttcg
     6181 atctctacgc cttctttagc ttcgattgct tggtgaagtc catctgaata acggcgtcca
     6241 tccatgatcc gaccagtgaa ctggtcaaca atcagtactt ttccgtcttg gacaacataa
     6301 tcgatatcta aaagcatgat atagttggct ctcaatgctt gatccaagtg atgggtcaat
     6361 gcagtatttt caatgtcgta taagttatcc aaaccgaatg tttcttctgc tttttcgatc
     6421 cctgcttcgg ttaaactgat tgtttttgat tgtacatcga ttttataatc ttcttcttct
     6481 ttcaaacgtt tcacgaagtt gtctgctctt gtatataaag ctgtggattt ttctgcttgc
     6541 cctgaaatga tcagcggcgt tctcgcttca tcaatcaaga ttgagtcaac ttcatcgaca
     6601 atggcataat tcagcggacg ttgtaccatt tgatggcggt aaacgaccat attgtctctc
     6661 aagtagtcaa atcctaattc attattcgta ctataagtga tatcacaatt ataagcctcg
     6721 cgtttttcat ccgaagattt tgagttgata ttcaacccca ctgttaatcc taagaaatta
     6781 taaagttcgc ccatttccgt cgagtcacgg gttgctaaat attcattgac ggtaactacg
     6841 tgtacccctt cgccagtcag agcatttaga tatactggca tcgttgcggt caaggtttta
     6901 ccttcaccag tacgcatttc agggatattt ccatcgtgaa gcacgattcc acccatcaat
     6961 tgtacgtggt gtggatataa ccccaataca cgttttgctg cttctcggac gactgcaaaa
     7021 gcttccggaa gtaattgatc taatgtttct cctttttggt atctgccctt gaattcagca
     7081 gtttttgcct gtaattgttc atcagttaat gctgccattt ggtcagcgta tgcttcaact
     7141 ttttttgcaa tgctatctaa acgttttagt tctttcttat cgttttcgat aagtgaacga
     7201 atcaaattgg ccatgcgttg tgtaatctcc ttttccttct gtgtattatc cagactatgt
     7261 agactttact tttcatttta tcattagtta tagataattg aaatatctta agcgtattaa
     7321 agtaagaact tcataattta ttcatatttc atctaaaaga ggaggaaact caaaaaaact
     7381 tgcggcagct gtcagtagct gctgcaagtt tt
//
LOCUS       DAZKBH010000116         7394 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000116 DAZKBH010000000
VERSION     DAZKBH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.118"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..418)
                     /locus_tag="ACG8OO_002427"
     CDS             complement(<1..418)
                     /locus_tag="ACG8OO_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFP9046273.1"
                     /translation="MNYYRNIDRTKIQFDFLTHRPYKSDYDEEIISLGGKVYYAPRLY
                     PQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKAAKKAGIPIRIAHSHNTSIDR
                     DFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDK"
     gene            complement(478..1614)
                     /locus_tag="ACG8OO_002428"
     CDS             complement(478..1614)
                     /locus_tag="ACG8OO_002428"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9046274.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1646..3097)
                     /locus_tag="ACG8OO_002429"
     CDS             complement(1646..3097)
                     /locus_tag="ACG8OO_002429"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9046275.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3141..3905)
                     /locus_tag="ACG8OO_002430"
     CDS             complement(3141..3905)
                     /locus_tag="ACG8OO_002430"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFP9046276.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(3933..4631)
                     /locus_tag="ACG8OO_002431"
     CDS             complement(3933..4631)
                     /locus_tag="ACG8OO_002431"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFP9046277.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4643..5422)
                     /locus_tag="ACG8OO_002432"
     CDS             complement(4643..5422)
                     /locus_tag="ACG8OO_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFP9046278.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5438..6382)
                     /locus_tag="ACG8OO_002433"
     CDS             complement(5438..6382)
                     /locus_tag="ACG8OO_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9046279.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6428..7207)
                     /locus_tag="ACG8OO_002434"
     CDS             complement(6428..7207)
                     /locus_tag="ACG8OO_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046280.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2051 a         1579 c         1150 g         2614 t
ORIGIN      
        1 atttatcatt tcgaaacaaa aaatcacctg ctacttttcc acatgaaaat tcatgtgtca
       61 aaacagacgt taacttacat cgataatatt gctttaaaag atatttaaaa tctctatcta
      121 tcgatgtatt atggctatgt gctatccgta tcggaatccc agcttttttt gcagctttta
      181 aaggtaaata actcatcgca tcaatatgag aatgaacaat ctcatattct ggatgatcct
      241 taaaaaattg ttccatccat ttaaaatatt taggataatt ttgtggataa agtctcggtg
      301 cataatagac ctttcctcca agacttatta tttcctcatc ataatcactt ttatatggac
      361 gatgtgtcaa aaaatcaaac tgaattttag ttcgatctat attcctataa tagttcatta
      421 gcatcgtctc caaccctgca cgatgcatat cattcacaca ctgtaatatt cgaatcatta
      481 tatatttccc ctatacaaat ttaatgtttc ttgtacgatt ttatcccaat tatatttact
      541 acaaataaac tcagtagctt gattcttcaa aactttaacc atttccgatt gattgcatgc
      601 ctcttctaat cttatcttta aatcacttac atccgatttc ttaaaaatca tagccttgtc
      661 ttctactact tccgtacact ctgagatatt tgaaactata caacagttcc cataactcat
      721 tgcttctaac aaacttaatg gcattccttc taaatcactt ggaagtgtat agatatacgc
      781 attactatat aattcttcta attcctgtcc ttgtacaaat ccagtaaata taattcgctc
      841 atcacctttt gctaattcct ttagttcatt tgcaaattca tctgtatcag agcttcctcc
      901 ggctattata agttttttat ctgtctgaac atctttaaat gcctcaatta gataccgaat
      961 tcctttttcg ggaaccaatc gccccaaaaa caaaatataa gaatcttttg ttaatccata
     1021 cttttctgta atcaatcctg ctgattttat ttcaattttt ttaacgccat ttggtataaa
     1081 ccttgttttt cttccatata tatcttcaaa ataattttgg acgctttcac taagtacaat
     1141 tatttcatct gcaaatttta cagcacactt ttcccctaac atgatgtatg tactagcaag
     1201 cttgttccat ttttctcttt gatgatctag accatgaatt gttgcaacac atcttttccc
     1261 aaaaagtttt ggcaaccaca acatagcaca tggtccttct gcatgaaaat gaacaacatc
     1321 atattttccg aatgctgctc taattccacc aaatactgaa gaactcattg ctgcaattcc
     1381 ttttatattt aatgtaaaaa tagtctttaa ttttacacct ttatattctt tatttttatg
     1441 ctggtcaaat tcctttccgc taacatgatg accacttcga ttatagcaag tcacttcatg
     1501 cccaagtgca accattcgcg ttgccaattc ttctaccact atctcaattc caccttctct
     1561 tgatggaatt cttttatgac ctagcataca gatttttaat ttttttcttg ccatcacgtt
     1621 ttactccttc tatagtatct ttatcttaca tggaaccgtt tctatgaagc acagttttga
     1681 ctgtcctaaa caaaattcta aaatctaatc ccatactcca attcctgata tattctgcat
     1741 caagttttac aacctcttcg aagtctgtga tatcacttct accactcacc tgccacattc
     1801 cagtaatccc aggtttcatc gcaattctcg cacgatgatg aaaatcatac ttctcatatt
     1861 ctgacttcaa gcaaggtcta aaccccacca ggctcatttt tcccattagg acattataaa
     1921 attgtggaaa ctcatccaat gatgtagatc gaataaattg tccaattcct gtcttctttg
     1981 ttccatcagg aagtatttta ttgccaatga ttcttgggtc aaaatccatc ttaaacatga
     2041 gattattact catcttattt tctttcatca actctgcttt gcgttcttct gcatccatat
     2101 acatactacg gaacttatac atcttaaatg tcttaccatt ttgaccaata cgttcttgtg
     2161 caaagaaaat cggccctggt gatttgatgt aaataactgg tgctacgaag ataaaaataa
     2221 cacctgttgc aatacatcca ataattccag caagaatgtc aatcgttctc tttataaatg
     2281 cttgcctcat cgtcataaca ttaatactag atgtaataac agtataatca ccaactcggc
     2341 caataaattg ttttccacgt gtactatcag acacttttgt aagattcata tgtacagcaa
     2401 gtcccatttc cgaacatctg gcgattaatt ctttgggata tggaacactt tcgtcaacat
     2461 tgatgaatac ttcatctacc caattctgta aaagatattc cgctgcatta tcagcatttg
     2521 caactactgg gacacctccg atggtagttc catacatatc tctgtctact acaatcacac
     2581 cactaagttt atacatgcca taattcttct ctttaagatt acgaactaca ttttttgcaa
     2641 tcttttgaga tgttacaata actaacgata catcttcacc gtttttcatg cggtgatgca
     2701 acaacccttt ccaaagtata cgaattatat atgttaataa tccatacacc acccccatta
     2761 taaataaaga aactctggaa taatcatttc catcctgcat tgtaataagg tataaactgc
     2821 acacaagtaa cagcataaaa gattgcttta tagaagctac agcctcttta taatatcctc
     2881 tttttaaaac atttttatat gcctcaaata aaaatataat cgtaagattc tcaagttcta
     2941 taaaaattgc catgtggcga taaataggaa tcacatacgg attccagtca ccctgtcgca
     3001 ttacatatga tatataaaaa gcaagctgga cacaaaataa gtctaataaa ataaaatcaa
     3061 tatgtttaga ccaaccactt gagttttttc tatacatctc tcactctctc ctcatttcct
     3121 ttttatctac gaataaagaa ctaaaataaa ccaaacttct tcttttttac ctcttcatag
     3181 attggataat tgatctcatc cccgttgatc agatcctttg cgacttgttt catctgtact
     3241 actttttcct taccaaactc cttggcaatc tgctcatagc actcttttaa gtaaaaggtg
     3301 cgcttagaga ccccgtgtgc atcagaagca gccatctgca cgagattatg tttgaccatt
     3361 gttttggctg ttttctggat atctttgcca aatacaccaa cataacttgg cgcagtaagt
     3421 tgggccaaac atcccatatc taggaacgga agcaaatgat ttgggtcttc tcgaaaatgc
     3481 gcgttgcgtt ctggatggac gatcactggt atctttccta gttctaacaa ctcaaaaaaa
     3541 agccgttctg catataacgg cacttccagt gtcggaaatt cgatcagaag atatgtatca
     3601 tctaaatctg taaataaaat ttcatctcgg tggatgtctt cgatgagcgt tccggtaata
     3661 cgtacttctt gtccttccaa taacgtcaaa ggcaactgtc ttttctctaa ttcggcttgt
     3721 aacgaagcga ctagagaaat gacttgtgat ttttcattgc tgtattttcc attattgtgg
     3781 tgtggtgtgc ataaaatatg cgtaatgcct tgctgaatcg ctttttcagc cattgcaata
     3841 cttgcttcta agttttctgc cccgtcatcg attcctggta ggatatggca atgtagatcg
     3901 atcatttctc ttgtctcctc tcttcttatt ccttaatttc cgtagtaata accagcatct
     3961 ttgctgtgtt ctgctccgtt gtatacgaca cctaagactc gtgcttgtac catatctaac
     4021 agttcttttg ctttagtcaa tgattctttt cgtgacgtat tttcccgtac gaccaataag
     4081 gtgccatccg tttttgaaga catgatctgt gcatctgtta cggcaacgac tggcggcatg
     4141 tcgaagatca ctacatcaaa tagttgtctt gcttctgcta aaatctgatc cattcttggc
     4201 gaacttagta gttctgatgg atttggtggt ttaggtccac ttggcagaat cgacaaattg
     4261 tcgactggtg ttcgttggat ctcatctgct acacttcctg atgaactaag aacagtactt
     4321 aatccgcttg cgttgttcaa cttgaacgtt ttatagatga cgggcttacg taagtctgca
     4381 tcgactagca gcacccgttg accagatttg gcaaaaacaa cggcaatgtt tgctgcagtg
     4441 gtcgatttcc cttctccagg tcctgatgaa gtcacaacaa tcgttttgat ttgctgacca
     4501 gctgcggagg caaactggat attcgtccga atcgtacgat attgttcagc gattggtgaa
     4561 gagggattgg tgagtgtgac caaactgact gcatgagttt gggctgcttt ttgtttctgt
     4621 gttcgtgcca ttttctttcc tcctagactc ttgagcggct tctgcgtgat ggcgctgttt
     4681 tatcgtttgt agcttttggt tgtgtctgag ctggtccttt tttacggata ccagcattca
     4741 attctttcgc tgacatttct ggaactgttc ctaagattgg gaatcctaat gtatctgtca
     4801 catatttttc atctttcact gtccgatcga gtaattccaa taagaatgcc agacctacac
     4861 ctaccatcat tcctaacatt aagccagtca ttaaattcaa tttattattt ggcgaaacag
     4921 gtgtcgtatt ggcaactgca cctgaaatga tcgagatttt atcaacattc atcacatctt
     4981 ttgcgttctc ttggaaaact tgtgaagttg tgttagcgat ctgttgagca gccactgcat
     5041 tcgtacttat tgcctgaatc gagaacattt gtgaattttc tgcctgatta actgagatgg
     5101 catctttgat ttgttcagca gtcattttca cattgtcttc tgtttctaaa cgatctttga
     5161 cttcattgag cactaaatca ctgacgatca tatctttata cgtattgatc atttgcaggt
     5221 tcgtattgac atcattgaca tttgcagtgt ctgattgtgg cagtgtcacg atcaattgtg
     5281 cttgtgaact gtattttggt gtcaagatga aaaatgttac gattcctgag atggccaagc
     5341 cagcaaacat tgagatgatg attattacca agtgtttgcg taatgtttca aaaatttctt
     5401 tcaagctgat tgtctcttcc attttttctc cactttccta tttattctct agattcattt
     5461 gatcttttaa ttcttttttc acacgatcaa gttcttgatc actgactttt tgataggaga
     5521 tattatcttg catgaagcct tctccttgca gctgatcttg cttaatcgta ccaaatgcct
     5581 cccgataatc taaagcaatt tttttcattt catcaaagtt catgtctgtt ttcatattcg
     5641 actctagagc attcaaaacg gattgatagt tgctgacacc gtttagactc aatacttttt
     5701 tcgctacgcc ttctacgatc tttctttggc gcccttgtct accgtaatca ccatttgggt
     5761 cttcataacg catacgagaa taagataatg cctgatcccc gttcaatgta attttaccga
     5821 ttggaaagtc atagccatct tgtgagaaag tgaggtcatt attgacttcg ataccaccaa
     5881 ctgcatcaac gagttccttt agccctttca tattgataga gacatagtga tcgattggga
     5941 tgtctacata tttttgtacg gtatccatcg ccatggcagc tccgccgaat gcgtaagcgt
     6001 ggttgatctt gtcttttgtg tcacgaccta cgatgtcgac atacgtatca cgaggaatgc
     6061 tgacgatcgt tgtctgtttg tcttcaggac tgactgtggc aaccatcatc gtatctgatc
     6121 ttccttgatc gacccgacct aaatctccag tatcaatccc catcaataga acagagaaag
     6181 attcttgttt gtccagatca actggtttcg ttcgttctcc ttgggttcgt tccactgatt
     6241 cgtaagtttt ctcaatagaa tgtgagagat ccagatataa ttttgctcct actcctacaa
     6301 ttgctaatac taatacgata agaactgcta aaataatcag aaatattttt ttccctcttg
     6361 attttcttct tcgtcttgcc atagtttcct ccgtttgcta accttgcttg aatcaacgtt
     6421 ttaactgtca atagcttata ttgctatagc ccatttgctc agcaacagtt tgtgcttttg
     6481 ttgcgtatgc tgcgcttatt ttttcattcc ggatctgctc tatttgactg agcaccgtca
     6541 aatattgatc gtaagtagcg gtggcagtca cttgaccatc ctttaacatc gtatcgaata
     6601 actgatctag ttgcgtgact tgcgcgatct gaatatcagc gaatcctaga tattgcataa
     6661 caaccgtttt ccaaggacta tctttaaaaa agctcatatt ttcacgtaca cgagcgacat
     6721 ctgcacttgt caccttttct ttgatgatga catcgtctac agcttgctgc cagtttgata
     6781 catttttagt aaataaatcg ttgacagctg tctggatctt cagttttgat tctgcactat
     6841 ctaactgtgt cacgattgct ttcttctcat cttggattgc aacagcttct ttaggcaatt
     6901 ctgattcttt gatgtcgtaa tcatctgctg aaagtttgac agaggatagc ttatttgaca
     6961 aacttgtgat tgtttcttct gttatttcag ggtttagaaa aattttttct tttgtactat
     7021 acagcttgtc tacttctttt gataaagaag tcaaggtgtc tttttgattg gtgatggcag
     7081 attctgcata attgatccgt tgctgatgtt gccgctgatt attgatatgg tatccaaaca
     7141 aacagatcgc aatagcacct gcacctaccg ccacagcagt caatgacttg acgaatggct
     7201 tttccataga ttcactccta agtattgatg atagataaat taagatataa ttcacttaca
     7261 taaaaatgac agctttttat ttaaaaaaac ataattaaac taatatattc acagaaatat
     7321 ttgtaaacaa gttttcccta tataatcata ccacaaactg ttttcttgac tagggcaatt
     7381 tacgatttaa aagt
//
LOCUS       DAZKBH010000117         7307 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000117 DAZKBH010000000
VERSION     DAZKBH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.119"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..856)
                     /locus_tag="ACG8OO_002435"
     CDS             complement(23..856)
                     /locus_tag="ACG8OO_002435"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9046281.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(1048..1752)
                     /locus_tag="ACG8OO_002436"
     CDS             complement(1048..1752)
                     /locus_tag="ACG8OO_002436"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9046282.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(1775..2401)
                     /locus_tag="ACG8OO_002437"
     CDS             complement(1775..2401)
                     /locus_tag="ACG8OO_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9046283.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            2727..5102
                     /locus_tag="ACG8OO_002438"
     CDS             2727..5102
                     /locus_tag="ACG8OO_002438"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9046284.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(5126..5617)
                     /locus_tag="ACG8OO_002439"
     CDS             complement(5126..5617)
                     /locus_tag="ACG8OO_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFP9046285.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            5804..6724
                     /locus_tag="ACG8OO_002440"
     CDS             5804..6724
                     /locus_tag="ACG8OO_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9046286.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            6878..7222
                     /locus_tag="ACG8OO_002441"
     CDS             6878..7222
                     /locus_tag="ACG8OO_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFP9046287.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT         2385 a         1319 c         1423 g         2180 t
ORIGIN      
        1 aagagaagtg ttctggtttt ttctacattg caataaacca ataaccaatc aaaatgatac
       61 caaggataat acgataccag ccaaaagcgg taaagtcgtt tttcttcaaa tagcttagta
      121 agaatttgat agctaagacg gaaaccacaa aagatacgaa tgttcctaca agaagaacaa
      181 cagtttcttc cattttgaat gaattccctt cagctaaata tttgacgatc ttcaagccgc
      241 ttgcgccaaa catgacagga atccctaaga aaaatgaaaa ctctgtagcg acaaaacgcg
      301 aagcaccaat caagatagat cctaggatcg ttgcgccaga acgggatgtt ccgggaacaa
      361 gtgccaagac ttggaataac ccaacaagaa gagctgcttg gtacgtaaag tcattcagag
      421 aagtccaacg aggtgttttg tttttgttgc gtttttcgac aataataaag gcaataccgt
      481 aaacgatcaa aacaatggca actggtaaga acttgtggaa atttgcttct aaccaatcat
      541 ccaaaggcaa accaatgatg atggctggta tgcaagcaac gatcacttta aaccaaagtg
      601 tccacgtatc ttttttttct accgttgatt tacttggaga aaatgggttt aacttgtgaa
      661 aataaagaac gacgacagcc aagatcgccc ctaattggat aacgacattg aacatcgaca
      721 taaattgtga actggcatcc agtttgatga attcatctgc taggatcaaa tgaccagtac
      781 tactgatggg cagccattcc gttattcctt caataatacc gagaatcact gctttgataa
      841 tatttgcaaa aaacataaat tccttccttc ccaattaaga tacatcttat agtataccgt
      901 tattttttga gaaacccaat ctgtttttta atattaagaa aaacttgaga aataaagcag
      961 ataagtacac gaagagaaaa aagaagacaa agccctcgtt cagactttgc cttttatgat
     1021 tctctggtaa aaattaaaat atccccatta tagaaacgag tcgcgactat gtgttttccc
     1081 gttatcgata taagacgtat cattgatgcc gtggatagaa aatcgaccgt ttgcataagt
     1141 gacctttgtt acgctgccat ttaaaagccc agcacgaaca ggctgtttag gatcgatcag
     1201 attaagcaaa aaggcgatcg tcaaaccatg agatacgact aaaacatttc caccaccatt
     1261 tttttcgcag tgatgggcga tatcttcaaa tcctgtccat acacggtctc gcaatgtttc
     1321 ataagtttcc gcccacccag cggtatctaa gttgtataca gcattagcaa tttgttcaaa
     1381 agaaacttct gtggtaaaca tttcatcata tgttttaaaa ttcaatacac gagggatcac
     1441 accccacatt tccatgtcat atccaccttc aagagacccg aaacaccatt ctctaatcct
     1501 tttttcaata aaataaggaa tttcttttcc cttagaatgt tctcctagaa taatacgggc
     1561 agtttcgatt gcacgaccac tgtcactaga ataagcaaat gaaaagtcga cattttttaa
     1621 cccgcgacct aaataatgaa tgccttcttg tccttgcttt gtgaggggtg tatcacacca
     1681 gccttgtact cgttcaattg tattgaacat cgtttttccg tgtcgtacga tataaagtgt
     1741 agtttctgtc attttattct cctcctaagt cgttttaacg attgaaaaaa ggatttgtcc
     1801 ttttttcctg ttcaatcgtc gttgcatcgc catgtcctgg atagactgca aattcatcag
     1861 gtaatacaaa cagctgggtc cgaatactat gaagtaattg ttctaaattc cctgtataca
     1921 gatcggttct accgatactt cctctaaaca gggcgtcgcc agaaacgaca aaatcatcaa
     1981 aaataaagct gacgcttcct atagaatgcc ctggggtagg gacgacgcga aaagacatgt
     2041 ttcctaaagt ataagttttc atttcaaatt catattctgc aggcgagaca ataacatcag
     2101 caatatcatc atgtctaccg agtccagaaa gattcaaaat cggatcacct agccattttt
     2161 gctctagtgg gctgacataa actgggattt gataatacgc gcgtaaaggt tccactgcac
     2221 cgatatgatc gtagtgagta tgggtaagca aaatcgctac cggctgttgg tttgtttttt
     2281 tgatttcttg tttgattcgt tccgcatctt ctccagggtc aatgatcaaa agtgcttctt
     2341 cgttgtagac caaataacag ttttcttcaa tcattcctgt ttttaatctt tcgatatgca
     2401 tgtgcattcc tccttgatag ccaaaacttc tttcatccat tagtataacg cattcattgc
     2461 taggaaacaa aaacaggtac ttatttctct ttacttatct gaattatcag actatatgaa
     2521 aagtatttgg atattctaaa gagaaggaaa gtaagttttc atagcatttt aatatgtcct
     2581 tgttacatta gattcatcca aaaatccaaa ttctatatcc tatatccctg cctaatcagc
     2641 agggtgtttt tttatgtttg gaaagagagc tctttttgct aaactaggtt ttttcaggta
     2701 atcttagaga gaaagaggtg gtggaaatga tcaaacagtc aaaaatatct gtgcggcact
     2761 tagtagaatt tattttgcga cgtggaagta tcgacaatcg taaaaaaagt aaccatactg
     2821 cattagaagg agcgagaatc caccgcaagc tccaaaaaga tgcgggagaa aactatgaaa
     2881 aagaagtctt tttaaaaaca acagttcaat tagatgaata ttacctgatc gttgaaggcc
     2941 gtgcagacgg tattttcaaa agagacggta tttattatat agatgaaatc aaaacctccg
     3001 aaccacgatt tgaagattta gaaccagaac aagtggaact tttctttcat caagcaaggg
     3061 tgtatgccta tatctatagt caagaacatg atttacaaga aataaatctg caattgactt
     3121 acttccagac ggcagaagaa gtgatcacga gaaaggttga acatcagaca agagagcagt
     3181 tagaaacttt ttttaaaaag ctgactgaag attataaaga atggcttgtt tttcaagaaa
     3241 actggcgcac tgtacgcaat gcctctttga tggctttgaa attcccccat gatgagtata
     3301 ggaaaggtca gcgtgaacta gcggtagctg cttataaaac gatccgtacg aaacagaaat
     3361 tgtttgtaga agcacctacc ggaacaggaa aaacaatctc tacgttattc cccgctttga
     3421 aagctgtagg ggaagaagag ggagaaaaga tcttttatct aacggctaaa acaataacta
     3481 gacaagtagc tgaagatgct atgacagcat taaaagatac aggagctgaa gtaaaaagtg
     3541 tcactttgac ggcgaaagac aagatctgct ttttagatga gacgacctgt aatccagatc
     3601 agtgcccata tgccaatggt tattacaatc ggattaatga aggtttatgg gatcttttga
     3661 atcatgagaa tcagatcact cgtgaagtca tcgaaactta tgcaagaaag catacacttt
     3721 gtcctttcga actatcatta gatgtcagta tctggtgtga cgtaatcatt ggggattaca
     3781 attatttatt tgatccaact gtttatttaa gacgtttttt tgaagatgaa aaaaacgagg
     3841 actatctgtt tttgattgac gaagcacata atttggtgaa tcgttcccga gagatgtatt
     3901 cggcagaact atcttatgaa aaaacgaaac gaagtaagga agctatacca aaggaattca
     3961 aaaaacttca tcgccgtttc aacaaactat taaaagaatt tgattcgatc cgagaaatag
     4021 caaaagaaga tcattgggat tatcatcatc aaaaagcacc ggctgaatct ttagtaaagg
     4081 caggttatca attaagcgaa aaaattaaag aatggctggc agaatttcct gagcacccat
     4141 cacaagaaca attgcttcct tattattttg atttgcttca ttttttgaag gtcagtgaat
     4201 tttatgatga tcactacgaa acaacagtcg aaaaaacgta tcgcgatctg atagtaaaag
     4261 agttttgtat tgatccaagc ttgtttcttg agcagtcgtt ggataaagga aaaagcagta
     4321 ttcttttttc tgctagtttt tcaccactct cttattatca ggaaacactg gggggacaaa
     4381 aaagtctggc ttacaaactg ccgagtcctt tcccagaagg aaaccagcaa gttttcatcg
     4441 caaattacct agagacgact tatcgaaaaa gggaacacag tctggcaaaa ttagtagaga
     4501 cgattcatgc ttttgctgat ggaaaggtcg ggaattattt cgttttcttt ccttcttatc
     4561 aatatttaga tcttgcggtg gaagcattca gacagcaata tcccgacagc aatgtgctaa
     4621 tccaagaaac ggacatgaat gaggaagaac gcgaacgatt tttaacaaaa tttcaaacaa
     4681 atcccgctga aacattgatt ggattttgcg tacttggcgg gattttttca gaaggaatcg
     4741 atttgagagg ggaccgattg atcggcagta tgatcgtagg tgtaggtttg ccgcagatga
     4801 atcatgagca ggaactgatc aaatcatatt atgatgagaa ggaacacctt ggatttgctt
     4861 acgcttatca gcttcctgga atgaacaaag tcttgcaagc tggtggtcga gtgattcgtg
     4921 gcatggcgga tcaaggaatc gttgtattgg cagatcaacg attttctagt ttttcttatc
     4981 gacgtttatt cccacaacat tggcaaacag caagagaagt acgaagtgtt ggggaattgg
     5041 aagaaggaat acagcgtttt tggttatcaa aagaagcagt aaatcaaaaa aacgaggaat
     5101 gacagacgtc agcctcgttt tttgattaac gatagttagt gaattgcaac tcgattggta
     5161 agtcaagttt acgtaataaa gcaatcacag cttgtagatc atcacggttc ttacctgtca
     5221 ctctaatctg atcttcttgg atttgtgctt ttactttcag tttttcattt ttgatagctg
     5281 tagtgatttt cttcgcgttt tcacgatcga ttccgttgac caaatcggca gtttgtcgag
     5341 ctttgccacc tagagcgtgt tctgattcgg aaaaatggat atttttgatt ggcacatttc
     5401 ttttgatcag cttaccaaat aatacatctt tgatctgttc gattttgaaa tcatcatcgc
     5461 tcaatacaac tagattgctg ttctctaatt tgatttctac tgtagaatcc ttgaagtcaa
     5521 agcgattgga caattctttt gttgtttgct gaatcgcatt ttttacttct tctgtattga
     5581 cttctgatac aatgtcgaag ctagcatctt ttgccatagg aaccctcctg atatacatat
     5641 aaatatttac catacatcta tcttatcata ggattagggc agattcttac ctgtttgcta
     5701 aaattcttct catttgagtt cgaagtattt tattttaata gataaactgt tattcttaca
     5761 tttggaatac caaagtaggg gagaggtgct tgtgagtaaa attatgactg cgtatcataa
     5821 aaatgaaaga ttaaagaaaa tagtggttat tttgataacc ggattgaccg ctgcggtcgc
     5881 tttaaattat tttttgattc cagccaatgt cttttctgca gggatgaatg ggatcgctca
     5941 aatcatttcc tcgcttttat atgaatggtt tcatatcaag atagacacag gaacatttat
     6001 ttttcttttg aatatccctg tgtttgtatt agggtttctc aaagtaggaa aacgagcaac
     6061 gatccttagt tttgtgaatg ttgtatgtgt atcggtgatg acgatgctgc ttccagtcgg
     6121 acagatcact gataatattt tgatgaatgc cctagtaggc ggtgttttat taggtcttgg
     6181 cgtaggatta tctttaaaaa tgggctttac aactggtggg atggatatca tctctcttgt
     6241 actttcacaa acaactggaa aaacagtcgg aaactacatg ctgattctaa atggtatcat
     6301 tgtagtcatc gcaggcttct tctttaactg ggaaagtgcc ttgtatacga ttatttccat
     6361 ttatgcaatg actcaagtaa ttgatgccat ccacacgagt catcaaaaag taacagcaat
     6421 gatcgttacc gtcaagccag aagaggtaac agaagaacta gcaaaacgga tggtacgtgg
     6481 aatgacatta ctgccgtctg tcggtggcta ttcaaaaaaa caagggaaca tgatcatgat
     6541 ggtcatcacc cgctacgaaa tgtatgatct tgatcagatc gttcatgaaa tcgacgacga
     6601 tgcatttatc aatattttac caactcattc agtatttggc cggtttgcaa atgaaaacga
     6661 acaaaaaatg tatcgaacaa ctggcgtatt gccagaaata aaaaatcgtt cacaaaaaaa
     6721 atgaggaatc aggtttattt tagaataaat ttaatctatt acatctaata agaacatttt
     6781 tataaattta aacgagaatc ttacttctaa attctcattt acaagcagct aaaaaaagag
     6841 tatgataaat aaaaatcgtg ttaaggagtg tcgtacgatg agcgaagcaa accaagaacg
     6901 gtctggacaa gaaatagaac taatcaaaga acgtattctt gctgccttgg aaatggtgat
     6961 tgatcctgaa ttaggaatcg acatcgtcaa tttaggcttg atttatgata tagaattcaa
     7021 tcctgaaaat ggagaaacag tcattaagat gactctaaca actatgggct gtccattagc
     7081 agatatttta acagaatcta tccatgacgc attaaaagaa gtgccagaag taacgaaagc
     7141 agaagtcaaa ctagtctggt atccagcatg gacgacagat aagatgagcc gttatgcacg
     7201 gattgcgttg gggatacgat agaaatcttg gctgactagc tatgagaagc aaataggaaa
     7261 aacaaaaaag ccttttatcc tttctagaag aaaagagata aaaggtt
//
LOCUS       DAZKBH010000118         7281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000118 DAZKBH010000000
VERSION     DAZKBH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.120"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..153
                     /locus_tag="ACG8OO_002442"
     CDS             <1..153
                     /locus_tag="ACG8OO_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9046288.1"
                     /translation="ENFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHI
                     YMKKTL"
     gene            complement(231..764)
                     /locus_tag="ACG8OO_002443"
     CDS             complement(231..764)
                     /locus_tag="ACG8OO_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFP9046289.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(858..2036)
                     /gene="mutY"
                     /locus_tag="ACG8OO_002444"
     CDS             complement(858..2036)
                     /gene="mutY"
                     /locus_tag="ACG8OO_002444"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9046290.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2029..2826)
                     /gene="recX"
                     /locus_tag="ACG8OO_002445"
     CDS             complement(2029..2826)
                     /gene="recX"
                     /locus_tag="ACG8OO_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9046291.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2933..4315
                     /gene="rlmD"
                     /locus_tag="ACG8OO_002446"
     CDS             2933..4315
                     /gene="rlmD"
                     /locus_tag="ACG8OO_002446"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9046292.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4489..5067)
                     /locus_tag="ACG8OO_002447"
     CDS             complement(4489..5067)
                     /locus_tag="ACG8OO_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFP9046293.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5389..5859
                     /locus_tag="ACG8OO_002448"
     CDS             5389..5859
                     /locus_tag="ACG8OO_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFP9046294.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5967..7193)
                     /locus_tag="ACG8OO_002449"
     CDS             complement(5967..7193)
                     /locus_tag="ACG8OO_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9046295.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2103 a         1461 c         1250 g         2467 t
ORIGIN      
        1 gaaaactttg ctaagaatca aggctataat acgattttat taggggcaca gtccacagct
       61 gaaacttttt acgaaaaact aggatataca gcttacggcg atccttttga agatgctggc
      121 atgcctcata tttatatgaa aaagacttta taaaaattta tttcgaaaaa agcaaaaaac
      181 agcaagcttt tgagaaaaat ctctcactgg cttactgttc ttttttgtgt ttacttacgc
      241 gacaattgct tgtaacggtt ataccaaata tcaatatagc cttccgagaa cgggccatct
      301 ccattattga tccagtcaac cagaatcttc acattttctt ttaggatgaa gtcaatatcg
      361 ttcgggtaat tcatcatttt gctgtgcatc tcatattcat cgacatccaa tagtcgtttc
      421 tcgccgtcag gaaatacctt gatatccaag tcatagtcga tatatttcaa ggcctcatcg
      481 tccagcaaaa aaggtgaagc tagattacaa taataggaaa cccccttttc tctgatcatt
      541 gctattacat tgaaccagta ttttttatga aaataaacaa tcgctggttc gcgcgttacc
      601 catcggcgtc catcagattc agtgactaat gtgtgatcat tcacgccgat catagagtgt
      661 tcgcttgttt ttaataccat ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg
      721 taactttgga tcgtcacaaa ctctccttct tttggaactc ccatagcaac ccttccttaa
      781 gcagcatttt gtgctttgtg tttgggtgtt cattacaccg acatcataat tattatatca
      841 ataaaaatga gctttgccta gaaaggattc ctattgtttc gatttttctc taactgatct
      901 accagtttca tttggacctt aggaaaaacg accgaatcaa atgcatcagg cttcaaccac
      961 ttcgaagttt tgttctcggt aaattcttgt tctgctccct cggttgcccg tccgtaaaaa
     1021 aggagaacat gccatttcag atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc
     1081 tgccaaacaa agagttcctg cttttcaaat gggagatttt gctcttcttc tgctactagg
     1141 tcatcaaaca gatcgatttc tggctttgtt tcccattctt ttttcaatcg ctcatactcc
     1201 tcttgtgtca cttccatcat tggaaatgtc cacatattcg ccagcaattt ttggctatct
     1261 ctttcttcaa aataatacgc tccagaatga ttttgaagag ctgcactgat ataataaacg
     1321 tcttttggct ttgctttttt tgtttttaca ggaaaactcg tttggattcc tcgtttgtta
     1381 gctaaacaaa aggcttggat cggacaagtc tcacactttg gagaagttgg tgtacagata
     1441 gctgaaccta gatccatcat tgcttggttg aattcacctg gatgcttttc atcaatgatt
     1501 ttccgcatcg cttcatcaaa gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg
     1561 caaaacaatc tgctgactac tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca
     1621 aatgcgatac ttgcaatcgc gcctgtcgta tatggtccta ttcccttcaa tgagctaatt
     1681 tcttcaggcg tttgcggcat ttccccatca aattctgaca tgatctgttt agcagcagct
     1741 tggatgttcc gggctcttga gtaataacca aggccctccc atgcttttaa aagcttttct
     1801 tctggagcat ttgctagctc ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa
     1861 tcgatcactg tgtctactct ggtttgctga agcatgatct cagagatcca gatacgataa
     1921 ggatcacgat tatagcgcca aggcaggttt cttttttctt gttcatacca ttgaatgaac
     1981 tgatcttgaa attcttttgt ttctttatct gtccattttt cccattcttt attcactttc
     2041 ctcttccttt tccgtaatga atcgctggat catatccaaa tcaaatcctt tttgatataa
     2101 gctttgcttt acttttagat ttcttttttt aggttcaaac cttaaatttc ttcgccatag
     2161 tttatctcct tgtttgacta aataatcata ttctatttct tgatctgctt cttgcggaag
     2221 atcagctatc gcttgttgga tcacttcttg agaaaagcct ttcgtcatca atgtttgttg
     2281 gatttttctc aataattctt tttggctttt tccgtgattt tttccaaacg ctttttcggc
     2341 tgtctgatgt gcattttgaa cttggtcttc aaatgtatat tggctgagcg cttgttcaat
     2401 gatttcaggt ttcaaacctt tttgctgcat ctgctgcgct aatttctttg gtcctttgtc
     2461 acttaagcgc atattcgtgc gcacataact ttccccatag accaaatcat cgacaaggtc
     2521 caattctttt aagcgggcta cgattttatt tcgatcttct tgaaggattt ccttttcttt
     2581 taagtatgtt cggatttctt tttccgatcg caactgataa cttaagtaat tcattgctag
     2641 gtgaaaaccg aaatcagctc cgctactctt cttgatctct tggatagttt cttcgtctaa
     2701 ttcccgtcct tttaataatc gatagcgtac taatacatct tcagatactt gtaaagcttc
     2761 tccttcagat aaatctacgc gatagaaagg gcctttgcct ttactgattc tcgtaacggt
     2821 taacataaac atcctcctcg aacatatatt ctctatcata ccacagaaaa tccccgtaat
     2881 caggtattat atgttagaat aaaccacaga aaaaagaaaa agggagatac ttatggcaga
     2941 aacaattgtc aaaactggac aaacggttcc tttgaaaatc aagcgtttag gcataaatgg
     3001 tgaaggaatc ggttatttca aacggttgat catatttgtt ccatatgcac taccaaaaga
     3061 agaagtacta gtgaaaatca cgaaagcaac tcctcgctat gcagaaggag aattaataaa
     3121 agtcaaaaaa acaagcaaag atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg
     3181 tggctgtcaa ttacaacact tagcttatca agctcaattg gattttaaaa aggatctgct
     3241 tttgcaagca ttggaaaaat tcaaaccagc aggctttcga tcttatcaac ttttgccgac
     3301 gatcgggatg gatgagcctt ggcattatag aaacaaagca caatttcagt tacgaaaaaa
     3361 taagcagact caaaaaattg aagctggttt atacgaagca aactctcatc agttagtccc
     3421 tataacagac tgcttggttc aagaacctcg aacacaaaaa gtaatgaata ctgttgttca
     3481 actattgaat aaatttgatg cacccatcta tgatgaacgg acaaacagtg gaatcttccg
     3541 cacaatcatg gttagaatcg gtgttaaaac tggggagtta caagttgttt ttattactcg
     3601 cagtaaaaaa ttcccgcaaa aaaacgcaat gatccgtgag atcaatgaac gactgcccga
     3661 agttgtttca atcatgcaaa atgtccaggc acaaaaaacg tctctcgtga tgggggatga
     3721 aacgatccac ttatggggaa aagaagccat tgaagaacaa atcaatgaag tgactttcga
     3781 tctttctcct cgtgccttct ttcaactcaa tcctcagcag accgaattgc tttatggcga
     3841 aggaatcaaa gcattagatg tccagcctaa tgaaacgatc gtggatgctt attgcggagt
     3901 tgggacgatc ggcttaagtg tagcaaaaca agccaaagaa gtacggggaa tggataccgt
     3961 tcctcaagca atcgctgatg caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg
     4021 atatgaagtc gggacagcag agcaagttct gcctaaatgg ttgaatgaag gttttcagcc
     4081 tgatggtatc atcgtagatc caccacgtac gggtttggat aacgcattga tccaatcatt
     4141 attgaaatac cctccaaaaa aacttgttta tatctcctgt aatgtttcta cacttgccag
     4201 agatctggtt aatttaagta aaaagttcaa gattgaatat ttacagtccg tcgacatgtt
     4261 tcctcagact gcacgttgtg aagttgtcgt gaagatgacg agactcgctg actaaatgaa
     4321 agaggttgtg aaaaagtctg ccctgtatca agtaataaga acagccaaac aaaaatagct
     4381 cttcatgttt tatgaatact gtacttgtga ccgaagagtt aacaggagga cactatttat
     4441 ttaaaaaaag ggttgtgaca tagtttttgt cacagccctt tttgattatt attggatatt
     4501 cacgtctatc acttgataaa atgcatttgt cgtatcgctg atattccata ctgctaaaat
     4561 cacattatat ccttttcgat cctgcggaag tgtgatcgtt tggttgactg tttttggtgg
     4621 aacagttgct ttgtcgtcta tttttgtgat caattcaaaa tcactaaatt tcaaaggttg
     4681 attaggattc caagttggtt tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc
     4741 tgttaaattc caagtaatat ttaatgttcc tggattaacc atgtttttat gccatctgtt
     4801 agatgtctgt tcatctaaag gctcaaagcc gctgactcct gcactagcaa tttttccatc
     4861 aatgaatgta tttttggtag cttcgatact ttgcggttcc cattgtgctc ttccgacatt
     4921 ttgatttaag tttcctgctg ctgatgttcc taggtatgca cggcttcctg gagaagtaat
     4981 ataaccatgc gcaaaacctg gaatgctaaa accgaacaac cctaaactaa ctaacccaat
     5041 aaacaataac gtactaatct ttttcatgta gaaacctcct tcatttgata accacattgt
     5101 aaacgcttta ttgtattaac acaaacatta tgcttttata attttatgtt gaagtaaaaa
     5161 aggtgaaaaa agtaagatgc agattataac agaagtagac gagaaaagta caaatagaaa
     5221 acatgagaat caaatcatat gatctttttc aaaaacatca ttcatataaa caaatgttca
     5281 actttttaat aacaacaatt atcaattttt ttcttacttc acaaactaaa tacaggtctt
     5341 gctgtgagcc ttcatggtaa actaatatta tagaaaggag ttagtaaaat gaagatgata
     5401 aaagatattc gtttttgggt ttgtgttata ggaattgtca tattaggctt tttatcaggg
     5461 ttgcttagcg gaaatcccgg aaaatattac tattctttgc aacttccacc atttgcacca
     5521 cctagctgga tatttgggcc gatgtggacc ttactgtata tattgatggg tatttctctt
     5581 tatttgttac ttaatcacaa caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg
     5641 attcagttca tttttaattt tatatggtct gctctatttt ttaatttaag aaatattttc
     5701 attgcagcaa tcgatattac acttttagtt attttcttgt cagtgcttat gtatcagttg
     5761 tggctgcatc atcgcctgac aatgtggctc atgattcctt attatttatg ggtactattc
     5821 gcaactctat tgaattattc aatttatttt ttaaactaag cgctaaaaag agacgtcctg
     5881 taatttaaca catagaaaaa gtgccaccta agttagattt tttgtctaat ttaggtggca
     5941 ctctttatta accaaactat ttttttctag tacccacttg ttgttccata ctcattattc
     6001 tggtcataac cataaccatt ttgttcctgt ccagcgtaag gatcataact ttgagtatct
     6061 tgacttggtg tatagtactg acttgtatct tctgtttgct gataataggt attcggatct
     6121 tggtactgat catatgtgtt ttctgaatag gaagaataat cggtgtaccc ttcactattc
     6181 gatccatcac cgtttgtcga atcgccatct tccgaatcat caaagaacat cattgaagca
     6241 ttctcatttt tcaaattagg taatgtatcg cttgttttta tatttaattg tttcttcagt
     6301 tcattttgga tcgaaagaag atcatttttt cctaaaattt gatagtagat atcattgatc
     6361 atttgtcctt cgccttgcaa ctggtcttgt ttgattgttt caaacgccgg ggtgtagttc
     6421 gtcgctacat caagcatgtc atcccaatct aagtccgtcg tcacattgtt ctcaactgct
     6481 tttaaaattt ttctataatt gcttacgcca tctaaactta aaactttatt gacgattttc
     6541 gtcactactt cccgctgacg acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc
     6601 attcgagaat aggctaaacc tttttctcca ttcaatgttt gttcacctac tggtacatgg
     6661 acaccatcca aagtaaagtc aattttatta ttcactttga tgccaccgac cgcattgatc
     6721 aaatcttcca tgccatccaa attgatcgtt acataatgat caataggaat atcgagcagt
     6781 tgctcaattg aatccatggc catttcgaca ccaccaaatg cgtaggcatg atttaattta
     6841 tctaccgttc cataacctac gatattcgta taaatatctc ggtccaaact gatgatcgtt
     6901 gatttttttt gtttcggatt aactgttact accatcatcg tatcagaacg tccttgttcc
     6961 gttcttccta atccaccggt atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca
     7021 tcaatgctta ctctctcaga acgtttggag gatattcgat tagagttttt agaaattttg
     7081 tttactgttt ggctcgcttc tcccataaat ctgattccgt atgctgataa tccggcaatc
     7141 agcaaaacga atacccctaa caaggttaat agaacttttt gccatcgctt cataatacac
     7201 ctctgtttca cattttctaa aatattgtct catataactg ttcaatcagc aaattcactt
     7261 tttaatcata agaaaacttt a
//
LOCUS       DAZKBH010000119         7053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000119 DAZKBH010000000
VERSION     DAZKBH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.121"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1778..2443)
                     /locus_tag="ACG8OO_002450"
     CDS             complement(1778..2443)
                     /locus_tag="ACG8OO_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9046296.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2569..4605
                     /locus_tag="ACG8OO_002451"
     CDS             2569..4605
                     /locus_tag="ACG8OO_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFP9046297.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4609..5247
                     /locus_tag="ACG8OO_002452"
     CDS             4609..5247
                     /locus_tag="ACG8OO_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9046298.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5275..5703
                     /locus_tag="ACG8OO_002453"
     CDS             5275..5703
                     /locus_tag="ACG8OO_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046299.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6160..>7053
                     /locus_tag="ACG8OO_002454"
     CDS             6160..>7053
                     /locus_tag="ACG8OO_002454"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9046300.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSS"
BASE COUNT         2415 a         1062 c         1303 g         2273 t
ORIGIN      
        1 caccccgttg ttatatgacc gtgttttgag aggaaccaat aaaaagcata ggctaactag
       61 gggttccttt ttcttttgtt tttttggaat ccgcttaagt gaaatgtatc tgaaatgaaa
      121 cggagataaa gtgatttttt gctataatga caaggtttta gctgtttcag ataccatgta
      181 acccaaaata gtttattgag aaaataggag agtatttgat actaactagt aggaaaaaat
      241 gagataaaaa ttaaatggaa tcatttcagg taatctcaaa taaaaaaata tacagtcctg
      301 tggcacttta cctatgggac tgtatatttt ttactctcag cgggcttgtt ttgactccgt
      361 gtctgactcc gtcgtattga ttttaaatga tattcgatga ccatttaaca ggtcattttt
      421 gaaatttttg tctctatatg actcagtttg ataattattg atagatcaat ttgggtctat
      481 atcgaacaag ggaataatcc aataaaaaat gatttagaat atcaaaataa caaaaaagag
      541 gactacctgt attcgtcctc ttttttgacc agattttgac cagtctaatt tacattctaa
      601 tatattcata tgagctaatt ttttgattaa atggtaagtt tttccttgtt tgttttagtt
      661 taaactagtg tgtaatttaa aaatctatat ggaagggtct gtggggtaat acttaaataa
      721 aggctaggaa cctgataaca tcaaggttcc tagccttttt ataataaaat gaagcgaaaa
      781 taatttcttt taaagagccg agattatcag ctatataaga gtgaatttga tgcaaaaaac
      841 agagccgaaa cagtcttggt gttcgtagct ctgcttatat gtttcgaatt taagacaaag
      901 agttggcaaa agatgttgat aaggaagata agataactgt gttatataaa ctaggtaact
      961 aactgtaaca agctaatttt aagagattct tattagaaga cgtttaggaa tataactaag
     1021 aaaatgatta tacaggtaat tgtcatttca ataatgtttt ctaattttcg cgtctttgtt
     1081 ttaagaaaaa ggccagaatc agttggaaaa aagttcgtac gatagtgaag aggggcaaaa
     1141 tttcctacta atattcccac taacaagaga taaaacggcc aggttggtgt gattcctcta
     1201 taaaaaaatg cccatgctac cgaaatccca tagattaaca actgaacaat ttttacaatg
     1261 aaaaaattgt tctgccatta ctcaaagtct cttcctcctt gtatcgttta tttgtttcat
     1321 tttatcatta tgataggcgc ttgaacaatc cattttgtat tttattgagt actcttggat
     1381 aaactaccag cgattatcat atttatttat gttactgtaa gccatcatat aaaagtaaat
     1441 gagcagatca tagaaatata aaaaagaact aaaattgatc taaattatgt gcagcttgac
     1501 tacgtaccaa tctatattac ttactagatt ggtcgttaat ctattcggat cgaatgagca
     1561 aaatattttt gtgaatgtcc atccttgatt atgattccaa aaagaatcat tgggaggctg
     1621 tggcgtaaat attatactat aagccagaaa tacctatcat tctgaacttt tttatttagg
     1681 aactttcatc tcaaggcttt ttcaattaat ttgtacccct gttttttgga taagatcatg
     1741 tgcattgatt ttttctatct ctacgttcat ctcctactta atgcctatct ggctcctgag
     1801 ggcttctttt taaggcgtcg tcatagtctt ggctgaggat ttcttggtaa tctgagggaa
     1861 tggcttccca agtgagcagg cgaacgttca tgacgtgttt tcctttgtgt ttgattacag
     1921 catggttttt ctctttttct acctctccag cgtgaaaaga cagccatgta ccatggccaa
     1981 agatatcatt acatacccaa cgttcgatgg aattgtccac gtatcctaca gggagtgtgg
     2041 tatagccgat ttgtggtttc aaaaaaggat tgaatcgatc gatcgtttca tttttgtatt
     2101 gataattgat aagaaaaaga tagaccaaac aaaagacgag cagaaagaaa gatagtgtct
     2161 ttttgagtga gaagggttgt tttgatgaat aagatttctt gttaacaaga ggttcatctg
     2221 gcaatgttgg ttctattgat tcggtagctg attcaataga aggaaaatca atcggcagac
     2281 ctaaagtatg atgtaaaagt gacagcattt caaggtctgg cattgtacga gaattttccc
     2341 aactagaaat cgttttatcg gaaacaccaa ttttttcgcc taattctttt tgcgtaagct
     2401 tacaatttct gcggtaagtt cgtatttttt ctccggtgtt catcttcttc tcctttcgat
     2461 tctacagtat atagatttat tctacatgac ctatacactt gatagatacg tttttttgat
     2521 gaaaaacggt atgattttat caaagaatca tttaagtaag ggagagtggt gataatcaaa
     2581 aaaaagttca cagttttact cacaatcata gtgaccggta tcatgagtgg ttttgccttg
     2641 gttagtataa ataaacaggt actctatcgg acaaatgaat ttttccatca aacagatact
     2701 tatactccga ttcgattgcc acaacatttt attgggcaag aagaatatga gcagctaatc
     2761 atggtatttg aaaatgtctc atcagaaact gagctttctt atatgaaaaa aacagtaaat
     2821 gaatccaatg aagtagatac atcttggaac ttcaagatac agccaaagct tgaaatgaca
     2881 ttgtattatc aaggggatgt caaatcttct cacgagaagg taccctattc taatacctcc
     2941 tttcgcgtaa cgtccattga agaatcgatc aaaataaaag attttgaagg tgaatttttc
     3001 ttgatgacga gagaaaaaga agcctatgaa cggttcattt cactattcac tgatcgattc
     3061 aatcaagcat ttcacactga ttatgccact aatatttttg aagactttga aaatagctat
     3121 tcgtattcgg ttatttcaaa tacagacgta tataattatc gagtgtattt aactattgga
     3181 ttgattcttt tattcctttt gttatcatcc tttttcttat tgatgaataa agaaatcagt
     3241 ttattgttga ataatgactt ttcattacat caagtattgt ggtgtttatt ggggaaaaag
     3301 aaaatcatta ttacattcat aattgcagga atcattggtg gttttatttt tatgtattat
     3361 aaggagcaat ttctttattt gttccagatg tacgttttct tgttcagcgg aatatatggt
     3421 atctctttga tttctctttt attagttgaa aaaatcgctt cgttaagaga agcgttcaac
     3481 tatatccaat acattctttt tacatttgga ttgttgtttt ctcttctttt gaccgttgcc
     3541 aatgtcgaat tagcaactat cgttttttct tcttcacagt tagctgtttc ccatgaacca
     3601 ccaaaagaaa tcgctggtga ggactatcat gtattttatc cagtaaccat cgggaaaaac
     3661 cacgtggatt ttatttattc caatcgtttt gcatcagcag cagatcagga attatatgaa
     3721 actttgaata aaaacggaag tatattagtc aatgtatcag attacttgaa tgaagaaaat
     3781 gaacagtttg gcagagggat aaaaataaat ctgaattatc ttaagaaatt tcctttgatt
     3841 gatgtctctg gaaggttgat ccaaataacc gaaaaagaaa cgcaccctgt tatattgatc
     3901 cctgaacgat atagacatac agatttaaaa aatgacctgg cgcaaataac agaatattat
     3961 caagaaatca gtgaaaaaga accgaaattt ctatttataa aaaacaatca acccatttat
     4021 acatttattc cttctatccc ctggatcgaa cattatccgg ttgttgaaat actgacattg
     4081 aaaaacagta tgtatttgga aagaaatatt ctcagcggag aaatctatcc accgttaaag
     4141 atcaagatag gctctttgtc gaaagaaagg ttatttgagc tgattgaaga aagtcagtta
     4201 cgtgataact tgcagttatc atttccgtat actcagactg aagagatagc tgtcaaagtg
     4261 ttaagcagat catttcatta cttgatacag atatttttac tcactttctt ttctttcttc
     4321 ttgttgagct atgtcatgtt atgcatatat tttgtctaca attgtcggaa aatcgctatc
     4381 tttcgttcaa gtggttattc gctctttgaa acgtataaag atttcttcat gatgaacctc
     4441 atcaaatggg gaacgacaag tgtaatcttc ctttttctta tagaaagaga gcctaaatat
     4501 ctttttaata tttttttctt tagcttcatt gattttattc tttcagttgt ttttattttg
     4561 tctacagaaa agaaaagtca gttattgctg atgaatggag gttaacaatt ggagccgttg
     4621 atagtgataa aaggtgcgaa aaaaatcatt cgaaacaaaa cgattcttca tttggatcgt
     4681 ttcactgcgg atgctgggga tcttgtgact gttgttggag aaagtggagc aggtaaaagt
     4741 acgttattga atatattagg attgaatgat cagttcagtt caggacaatt ctatttgttc
     4801 gatcaagaga cgaaacaatt aactgggaga gagaagcgct tgataaaaag aaataccata
     4861 tcttttcttt ttcaagattt tgggttgatt gaatatgaaa caatcgattt taatttagat
     4921 attggattga agttttcgaa tttaaatgtt tcagataagc gaacagcaaa gtatgaggca
     4981 ttgaaaaaag taaatataaa caagaagcta agtacgccta tctacacatt atctggtggt
     5041 gagaagcaaa gaatcgcctg tgcgagagtc ttattgaaga atagcaaaat cattttagcc
     5101 gatgaaccga ctgggtcatt ggataaaaga aataaggata aagtagttga aatcttatcg
     5161 caacaagctt cccaaggtac gttagttatc gtagtcacgc atgatccata tctgaccaaa
     5221 ataggaaaca agaagatcat gctttaaaaa tcaaaaagga aaggatgtcg ttttatgaaa
     5281 aaagcagtta ttatttgtgc actagggata ggttttgttg gaggaaacgc tatgtcatct
     5341 gaagtggaag caaattcagt tcacggtgaa atctattaca aatctttaaa gccaggagag
     5401 attcattttg atgctaaagg taatattatt aagtcaaatt taccagtaga ccgggaaatt
     5461 tctagaacag caattaatca tagctctgga agatgggttt attatagtga tacaacttgt
     5521 acgagaaaaa caggtcattc taaccactat agtcgtgtct ataatcgtca cggtgcaaaa
     5581 gcgaaagttg gaaactctat tcgatcagca aatgcaacaa aaggagtatg ggcctatgca
     5641 agtgcgacag gaaatagaac tgacacattc tcatgttggt acaatccaac cggttggtat
     5701 taagtatcca aaagtttata taaaatagaa aaaacatata atgaaacagc gttaaaagtt
     5761 atttttggta gttttaatat attcattttt agttgtataa aaatatatca taataaaaac
     5821 aacaaaaaaa tgtctggaaa atcgaacata aaagtgtctg atttgaatat cccagcagcg
     5881 gaagaagaaa aacaaaatcg ataacatttg ttctaagaat tagttacgtt aaatacggaa
     5941 cgcaagatag acttgaaaaa cggtaacaaa agaagtagta tattaaggtg tgatgtgcta
     6001 cttgacattt attctgctga gcgaactttc tggattgtgt agccttatat ctattcagga
     6061 cgtatcgagc agattagtaa aggtgttctt gatgattttc atcttgaaca cctttgtttt
     6121 tagcaattcg caaaaaacga aaagtaggag aattgataaa tgaaaaaaag atttttagca
     6181 cttgccatcg tactaggcac tggcttattg tcaggctgta cgaatgccgg tgaaaaaaca
     6241 gccgttagtt ataaaggcgg tacaatcagc gaacaagaag taatggatag cttgaaaaaa
     6301 atgcaaggag cagattccgc tgtgcaacag ttgatcgttt atcaagtatt tgaagataaa
     6361 tatggagatg acgtatcaac caaagaaatc gattctcaat atgatcaaac aaagaaacaa
     6421 ttgggtgatt catttgattc acaattgaaa tctgcaggtt acacagaaca aacattcaaa
     6481 gacagtatca aacaaagtct agcctttcaa gaaggtctaa agaaacatat caaattgacc
     6541 gatgaagatt taaaaacggc ttgggaatct ttccatccag aagtggaagc tcaaattatc
     6601 caagtagcaa gtgaagatga tgcaaaagat gtgaagaaag ctgctgataa gggagacgat
     6661 ttctctaaat tagccaaaga caagtcaact gatactacga caaaagaaga tggcggaaaa
     6721 gtgaaatttg attccactac tacaacggtt ccggcagaag tcaaagaagc agcctttaag
     6781 ttgaaagatg gtcaagtttc tgatgtgatc acttctacca atgcttcaac ttatactacg
     6841 gaatattatg tggttaaaat ggtgaaaaac caaaacaaag gcaacgacat ggataaatac
     6901 aaaaaagaat tgaaagaaat tgcaacagac acaaaattaa gtgacagtac cttccagaat
     6961 aaagtaattg gcgaagtatt gaaagacgct aatgtaaaaa tcaaagacaa agactttgaa
     7021 aatgttttga gtacattcac ttcagattct agt
//
LOCUS       DAZKBH010000120         6941 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000120 DAZKBH010000000
VERSION     DAZKBH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.122"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..535
                     /locus_tag="ACG8OO_002455"
     CDS             <1..535
                     /locus_tag="ACG8OO_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046301.1"
                     /translation="TIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEG
                     GEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGD
                     FNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTM
                     KNILKNVKIAESLDDIL"
     gene            532..1953
                     /locus_tag="ACG8OO_002456"
     CDS             532..1953
                     /locus_tag="ACG8OO_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9046302.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            1989..2705
                     /locus_tag="ACG8OO_002457"
     CDS             1989..2705
                     /locus_tag="ACG8OO_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFP9046303.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            2724..4307
                     /locus_tag="ACG8OO_002458"
     CDS             2724..4307
                     /locus_tag="ACG8OO_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFP9046304.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            4436..5020
                     /locus_tag="ACG8OO_002459"
                     /pseudo
     CDS             4436..5020
                     /locus_tag="ACG8OO_002459"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            4966..5484
                     /locus_tag="ACG8OO_002460"
     CDS             4966..5484
                     /locus_tag="ACG8OO_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046305.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            5507..6532
                     /locus_tag="ACG8OO_002461"
     CDS             5507..6532
                     /locus_tag="ACG8OO_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFP9046306.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            6781..>6941
                     /locus_tag="ACG8OO_002462"
     CDS             6781..>6941
                     /locus_tag="ACG8OO_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9046307.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         2533 a          798 c         1322 g         2288 t
ORIGIN      
        1 tacaatacaa attccattag ttacagctgg gtcaagaacc gcattgcaac tttcggatat
       61 ttttaaccct atgatagata tttataaaga aaaaaaatat atatttttta cgtctgttta
      121 tgattttgag ggcggagaac ctgtcggaga atatgaatta gtatgcaaaa tagcagactt
      181 agtaggaaaa gataatctaa ttgttaagac gcatcctaga gatgtaagaa caatttataa
      241 agataatgga tttatcgagg ataaaaattc atcaatacct tgggaggcga ttcaattgtc
      301 aggcgatttt aacgataaaa tatttatgac aattaattca ggaagtgtta tttcaggtag
      361 tacaatgtct gcaaaacctg tacgcacgta ttatatgtat aaattgtgta acatagaagg
      421 aaatccgtct tgcaaaaaga atgcgtatga tattgagcga ttgcttaagc aaaaaacgat
      481 gaaaaacatt ttaaagaatg tgaaaatagc agaaagctta gatgatatac tatgagcaaa
      541 actaaatatt taattaaaaa tatgggaata ttaacaataa gtaatttttc ttcaaaaatt
      601 ttagtttttt tgttagtccc tttatataca agtgtattat ccacaacaga atatggcaca
      661 tatgatttga caatttcaac agccacatta ttgtttccaa ttttaacctt aaacattgta
      721 gatgctgtga tgcgcttttt aatggataaa gataaaaata aaaaaaatat agcatctata
      781 gcgattaaag ttatattttt aagcgtgata gtctttggat ccgtaatgta tttgttaaat
      841 attacaaaat tatggcaaga tgtttatgga ttagaaaaat atattttttt gtattatata
      901 tcctatgtat ggaatcaatt tctaatacaa tttgctaaag ggttagaaaa agttaaagct
      961 atgggcatag caggtattat cagcacaatt gtaacaataa ttgccaatat tttcttcttg
     1021 cttattctga aatggggatt aattggtttt ttcattgcaa atattttatc acagattatt
     1081 tcagctactt atctgtcagt attagtgaga atatgggaat atatcgattt tgaggctaaa
     1141 gatcctcagc taaagagaga aatgctaaat tactcgattc cattaattgc aactgttgtt
     1201 ggatggtgga ttaatagtac agctgatagg tatgtggttt cctttatagt aggtgtctcg
     1261 gcgaatggct tattgtctgt ggcatataaa atacctcaaa taattaatac tttgcaggga
     1321 atatttatac aggcctggca aatttctgca attaaagaat atggagataa tgagactgca
     1381 aaattttatg gaaatacatt tgttatgatt aatttgttaa tgtgcataac ctgttcaagt
     1441 cttatcatat taactagacc gttttcacat attctttatg cgaaagactt ttatgaagcg
     1501 tggaaatatg ttccattttt attaatagct aatgttttaa attgtgcatc aggtctgtta
     1561 ggacctatct tatcagcaaa aaaagattct aaatccatga tgtggtctgc aatcataggg
     1621 gcattagtta atgtgattat gagtgtagtc cttgtttata agataggggt tcaaggtgcc
     1681 acaatagcca ctgttatatg ttcatatgta atatatgttg ttcgtaaacg agctgtggga
     1741 aaagatattt ttataaagca ttatcatatt gttgtggaat cttggggatt attgtttgtt
     1801 caggcaatca tagaaatata ttttaaaaat tatgtaatag aaattataat tatgattatt
     1861 ttaattatta taaatatcag ttatattaag cagttgtgtt atagaggaag aaacattatt
     1921 tcaatgatta agagtcgaga gcacaaacgg tagacgatgt tatttttata aaaagatata
     1981 atttattaat ggtcgataat tatgaaagga aaaatattct taaaaaaaag agtataggat
     2041 gtcgtatgaa aatagtcgct atagtaccaa tgaagttaaa taacaaaaga ttgccacaaa
     2101 aaaacacgaa agcttttacc aatggaaaac cattgtgtta ttatatactt tcaacacttt
     2161 taaaagtaaa acagattgat aaagtgtatg tctattgtag taacccagat attaaagaat
     2221 ttataccaga aggtgtaaca tttttaaaga gatctagttc cttagatcag gatacaacga
     2281 gtattacaca ggtattagcc ggtttcacaa aagaagtaga ggctgatgta tatgtaatga
     2341 cacacacaac agcaccattt attagcgaaa aatctattga aaaaggacta gatgccgtgc
     2401 tgacaggaga atatgattct gcatttgctg caaaaaaatt gcaagatttc ttatggaaag
     2461 acggtgaacc atttaattat gaattggaca atattcctag aacacaggat ttgccgcctt
     2521 tatacgaaga aactagtgga ttttatattt ataatcgcaa tgtgatgacc gagttgcata
     2581 gaagaattgg gaaaaaacct tgcatagttg aagtaggtga gattgagtca gttgatattg
     2641 atgaagccga ggattttatg attgctgatg cgatttataa tcatcttatt ttacaaggta
     2701 gttaagacag ggggaaggtt aggatgaaca atgtacagat tttagattgc acacttagag
     2761 atgggggcta ttgtaacaat tgggaatttg gatttgataa tacaaaaaaa attatttctg
     2821 gacttataga atcaggaatt gagattatag aatgtgggtt tctcacaaat aaaacagtat
     2881 acaataaaaa tgttacaaaa tatacgacct tagaagaatt agcacagata attcctgaaa
     2941 aaagagaagg aaaattattt gtagcaatga tgaactatgg cgaatataat atagaggatc
     3001 ttccagatta tgatggttca tctattgatg gaattaggat tgcgtttcat aaaaaaaatt
     3061 gcaatgaagc gttgaaatta tgtcgtgaga taaagaaaaa aggctactta gtttttgttc
     3121 aagctatggt atctctcaat tatacagatg aagagttttt agcagtaata aaaaatgtga
     3181 atcagtttaa accatacgct ttttatattg ttgatagttt tggaatgatg aaaggaaaag
     3241 atttaactcg tcttttttac atgattgagc ataatctaga aaaagatata cagataggtt
     3301 ttcactcaca taataacatg cagcttgcat attctaatgc acaaaaactt ataaccgtac
     3361 aaacaaatcg aagcctgatt attgattctt cggtgtacgg aatgggaaga ggagcaggta
     3421 atcttaacac agaactattt gtagaatatt tgaatgataa tgcaggaaaa aaatatcaat
     3481 taaaaccatt attatatata attgatgaaa tactaaatag tttctatcag aagagttatt
     3541 ggggatattc attaccaaac tatatttcag cagttcataa tgctcatcct aattatgcaa
     3601 gctatctgtc atataagaat actttaacaa ttgaagcaat gaatgaaata ttcgaaatga
     3661 tggatgaaga aaaaaaggta tcttatgata aacaatatat agaagattta tactacaaat
     3721 atatggaaac tgggaaaata caggaggaat atatggaaga actgagaaaa tttctctcag
     3781 acaaaaaaat tctactgatt gcaccaggaa aaagttctgt agatgaggta gaaagaataa
     3841 aagagtttgt taccaaagaa gaagtcgtaa taattagtgt aaattttgaa tattctcata
     3901 tagatataga ttacgtattt ttaagtaatt taagacgttt tcatgaatta ccgaaagaaa
     3961 gcagaactaa atgtattgtt acatcaaata tacaagcatc ggatgtatat ttacggacga
     4021 gttataaaaa gttgttatct gaaagggaag ttgtaagaga caatgcagga ttaatggcaa
     4081 ttagattttt agccgatatg aatgtggatg aaatattctt agctggattt gatggatatt
     4141 cacataatga agatgagaat tatggtgagc aatccatgga aattataaca aaatttgcat
     4201 tattagatgc aatgaacgtg caaatgagag atgaattgtc agaattagct aaaaaagtaa
     4261 aggtgacatt tttaacggat ccacgttggg ttcgaattga taagtagtag taataatgtt
     4321 tttaaaattt tgataaagag atagtgagag aataaaaatg ggtaaagtct taaataaaaa
     4381 agaagaaaaa tatttaaggg acgatggggc gagatgaaac tctaattttg aacttttaag
     4441 aatcatttct atattgtgta ttgttgctta ccatgctaat ttgcgtttaa attataatgc
     4501 agacggaatt atagtacatg gttcattaac tgctaaccag attttcttga attatattgg
     4561 tatttggggt cagctaggag ttaattgttt tatatttatt tcggcatatt ttttaagtga
     4621 gaaaagaaac attcactttg aaaatgtttt gggaattgta ttagaatgta ttttttatag
     4681 tgtattagtt tatggaattc tttgtgcatg tggaatagaa acattttcat tgaacagctt
     4741 tgtaaaagaa atattatcat ttgctctagg tagatattgg tttattacat cctatttagc
     4801 attgtatatt ctaagtccgt atttaaatat catgcttaac aatctggaaa aacttcagtt
     4861 gcaaaaaata agtatgttat tgtttatagg tgtatgctgt tatcgtatgt tatttaaaac
     4921 atctttgttt ggggattgtg ctgttttaat tgcactatat gtcatatgtt atacactgaa
     4981 aaaaacgata ataatatttt tgagagacac agaatcatag gaacaattgt atgtataaca
     5041 gttataccac ttctcatatc cataggaata cttttcaaat ttataccaaa tgcaatggtt
     5101 aaccagatgg ttcatattgg ttctccattg atggttatag caagtatatt tttattttat
     5161 gtatttaaaa atttttcatt ttacagtaag tatataaata aacttgcgag aaccagttta
     5221 ggggtatata ttttacatga aaatcattat ttcattgatg gtatgaagtc tagaggctta
     5281 atatgggatg ggatatttgg attaggcaaa aggtttttag atagggattt ttggatttgg
     5341 tttataattg cagttgtagt tgtgtgcata atttgcatgg ctatcgatgt tgtaagagaa
     5401 aaatttgaaa gacagatttt taaaattaag agtgtaaaga acctgtgtaa acttatgaat
     5461 catgttataa atatataccc ataattaata aatgaggaga aaagaagtga aagttatatc
     5521 attcggagaa ttattattga gacttgcagc acccggttat tcaaagttat ttcaaagaga
     5581 tagtttggag actacattct gcggagggga agctaatgta gcggtatcct tagcaatatt
     5641 tggatatcaa tcagaattta taacaaagct tccggattca gatgttggat atgcggcagc
     5701 taatgctttg cgttattttg gagtaaatgt tgataatatt atatttggag agggacgcat
     5761 gggactgtat tatttagaaa aaggagcagc tcagagacca tctaaagtaa tttatgacag
     5821 aaaattttct gcaatttcgt tagctgatag tgatgaattt aagtggaagg agatcctaaa
     5881 cgatgcaaat tggtttcatt ggacagggat aaatccagca ttgtctgaga atctctttga
     5941 gacgtcctta gatgcatgtc aggctgccaa aaaaagaggt ataacaattt cttgtgattt
     6001 aaattatcga tccaagcttt ggacatcaga ggaagcaaaa gaaaagatgg aacagatact
     6061 accattggtt gatatttgta tttgtaatga agaagatgca gaaaaagtgc taggaattaa
     6121 agctgagcat acaaatatta atagtggaga attaaataaa gatggctatg tgcaggtggc
     6181 tagggagatt cacaagaagt ttggatgtag gtatgtagct acaactctgc gtagaagtta
     6241 ttcggcaagt agaaatggtt ggagtgcaat gttatacgaa gcagaaaaag ataaagcgta
     6301 tttttccaca gaatacaaca tacaaattgt tgatcgcgtt ggtggtggag acagcttttc
     6361 tgctggtata atatatgcgt tatgtgaaaa tatgtccgga caaaaggcta ttgaatttgc
     6421 gactgcatct agttgtttaa aacatactat agaaggtgat tttaatcgaa ctacaattga
     6481 ggatgtatat aatttaatga atagcggtgg aaatggtcgc gtgttgcgtt agtcaacata
     6541 taaagataaa tcacagtaat ttttatggtg attatctgtc ttagtagtga ttagaataac
     6601 gtgaaaataa attgttgagc gtttatactg ttttgaaaaa taaatttaac atattgaaaa
     6661 atacaaatat atacgtatat tataaagcgc cgattgtaaa attaagctag acaactaaaa
     6721 cagtcatttg tgctacactc aaaatagttc cgaccaaaga attataagga gtgagtgcaa
     6781 ataacctata cctatcttac aacggatgaa cttgtaataa tagagtctta tttgaaaatt
     6841 aaccaatctg ttgctaaaac tgcgcattgc ttgaatcgtt caagtcaaac gatccataaa
     6901 gtatatctgt tcttcaagca aagaaaatca gccttagaat a
//
LOCUS       DAZKBH010000121         6885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000121 DAZKBH010000000
VERSION     DAZKBH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.123"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8OO_002463"
     CDS             complement(<1..182)
                     /locus_tag="ACG8OO_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9046308.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8OO_002464"
     CDS             517..951
                     /locus_tag="ACG8OO_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9046309.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8OO_002465"
     CDS             974..1294
                     /locus_tag="ACG8OO_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046310.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8OO_002466"
     CDS             1435..1626
                     /locus_tag="ACG8OO_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046311.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8OO_002467"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8OO_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFP9046312.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8OO_002468"
     CDS             2112..2375
                     /locus_tag="ACG8OO_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046313.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8OO_002469"
     CDS             2399..2587
                     /locus_tag="ACG8OO_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046314.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8OO_002470"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8OO_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046315.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8OO_002471"
     CDS             3070..3441
                     /locus_tag="ACG8OO_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9046316.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8OO_002472"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8OO_002472"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8OO_002473"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8OO_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046317.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8OO_002474"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8OO_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFP9046318.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACG8OO_002475"
     CDS             5250..5447
                     /locus_tag="ACG8OO_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046319.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACG8OO_002476"
     CDS             5553..6035
                     /locus_tag="ACG8OO_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046320.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACG8OO_002477"
     CDS             complement(6093..6689)
                     /locus_tag="ACG8OO_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9046321.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1059 c         1353 g         2142 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttt
//
LOCUS       DAZKBH010000122         6650 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000122 DAZKBH010000000
VERSION     DAZKBH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.124"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8OO_002478"
     CDS             99..746
                     /locus_tag="ACG8OO_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9046322.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8OO_002479"
     CDS             1139..3784
                     /locus_tag="ACG8OO_002479"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9046323.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8OO_002480"
     CDS             4094..5407
                     /locus_tag="ACG8OO_002480"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFP9046324.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8OO_002481"
     CDS             5397..6050
                     /locus_tag="ACG8OO_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9046325.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..>6650
                     /gene="radC"
                     /locus_tag="ACG8OO_002482"
     CDS             6105..>6650
                     /gene="radC"
                     /locus_tag="ACG8OO_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9046326.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGN"
BASE COUNT         2185 a         1136 c         1457 g         1872 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat
//
LOCUS       DAZKBH010000123         6643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000123 DAZKBH010000000
VERSION     DAZKBH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.125"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8OO_002483"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8OO_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8OO_002484"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8OO_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFP9046327.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8OO_002485"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8OO_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8OO_002486"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8OO_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFP9046328.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8OO_002487"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8OO_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9046329.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8OO_002488"
     CDS             3582..4271
                     /locus_tag="ACG8OO_002488"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9046330.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8OO_002489"
     CDS             4285..5787
                     /locus_tag="ACG8OO_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9046331.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8OO_002490"
     CDS             5800..6276
                     /locus_tag="ACG8OO_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9046332.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8OO_002491"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8OO_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAZKBH010000124         6570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000124 DAZKBH010000000
VERSION     DAZKBH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.126"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG8OO_002492"
     CDS             127..579
                     /locus_tag="ACG8OO_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046333.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG8OO_002493"
     CDS             593..1282
                     /locus_tag="ACG8OO_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046334.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG8OO_002494"
     CDS             1407..3758
                     /locus_tag="ACG8OO_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFP9046335.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG8OO_002495"
     CDS             3838..4233
                     /locus_tag="ACG8OO_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046336.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACG8OO_002496"
     CDS             4558..5175
                     /locus_tag="ACG8OO_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9046337.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACG8OO_002497"
     CDS             5216..5515
                     /locus_tag="ACG8OO_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFP9046338.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACG8OO_002498"
     CDS             5549..5716
                     /locus_tag="ACG8OO_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9046339.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="ACG8OO_002499"
     CDS             complement(5761..6366)
                     /locus_tag="ACG8OO_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9046340.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DAZKBH010000125         6478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000125 DAZKBH010000000
VERSION     DAZKBH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.127"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..1460
                     /locus_tag="ACG8OO_002500"
     CDS             42..1460
                     /locus_tag="ACG8OO_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFP9046341.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            1531..3000
                     /locus_tag="ACG8OO_002501"
     CDS             1531..3000
                     /locus_tag="ACG8OO_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9046342.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            3010..3477
                     /locus_tag="ACG8OO_002502"
     CDS             3010..3477
                     /locus_tag="ACG8OO_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9046343.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            3513..4385
                     /gene="scrK"
                     /locus_tag="ACG8OO_002503"
     CDS             3513..4385
                     /gene="scrK"
                     /locus_tag="ACG8OO_002503"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFP9046344.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            4466..>6478
                     /locus_tag="ACG8OO_002504"
     CDS             4466..>6478
                     /locus_tag="ACG8OO_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9046345.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKE"
BASE COUNT         2207 a          892 c         1304 g         2075 t
ORIGIN      
        1 attagttgtg aagataaaaa ccttttcact ggaggaagaa aatggagaaa ttattaatta
       61 aaagcaataa tcatcaactt tactaccaac ctaaggatgt ttgggtcgga gatgttatgc
      121 cttacgggaa agaaggaaaa ttctttattt atcatcaacg tgatactaga aatccagttc
      181 cttttggaga accgtttggc tgggctttag caactacgaa agattttgtt cattttgaag
      241 actttgggga atctctttta agaggggatg acaatgagag agaccagttt atctacgctg
      301 gttctgtatt tgaggcaaat ggaaaggttc acgcatttta tacagggtat aatcgtgaat
      361 tcttagcgca gggaaagaca tctcaagtgt tgcttcatgc tgagagtgat gattttgttc
      421 attgggaaaa atcagaaaca gcattggagt tagaaccaca agaagggtat gatattcgaa
      481 actggagaga tccatttgtc gtttgggatg atgaaaaaga agaatatttg ctaattttag
      541 gggcgcgaaa gggtcaggat aaacatctac aaaccggacg tttagtggaa tttacatcaa
      601 aagatttaac taattgggaa ttcaaaggtg atttttgggc tccgggattg tataccatgt
      661 ttgaaatgcc agaacttttc aagattggtg actggtggta tcttgtctat tcagaatact
      721 cagatggaaa taaaatactc tatcgaatga gtcaaagttt ggaaggtcct tggattaagc
      781 ctaaagatga agcttttgat ggacgggctt attatgcagg tagaacagct tatgatggaa
      841 aacgtcgagt cctatttgga tgggtaccaa caaaagatca agaaaatgat aaaaacaatt
      901 atttatgggg aggtactttg gttcctcatg aagtctatca aaaagaggat ttttcacttg
      961 gggtaaaacc catagacgaa gtgtggaatg cattcataaa tttacatgat attgccgatg
     1021 tagagataga aacagcagac acaagagaag aagtaacgct tgtcaaaaat agtggaactt
     1081 tgtttaattt agaaacaaca atttcatttg atgagaatct tagtgacttt tcactgagat
     1141 tatataaaaa ccttgagaca gatgaatctt acgaatatcg cttcaattta gatgaggggc
     1201 gtcttacatt tgataaaaac ccttgttacc cttggtttca atatatggat aaaggactta
     1261 ttagacctat atctttaaag cctaaccaat cttataaact aaaagtgatt gttgatgaca
     1321 atataatgac actttatatt gatggaatcg ctcttaacgc tcgtgtacat ggttgttttg
     1381 gtcaagcaat tactattaca gcaacaaatg gatcaatgac tgcaataaat accaagtttt
     1441 caaatgaaat aaagaaataa taaacgtcaa tcatgtggca ggtctgaaaa tcaaacatga
     1501 cctgtcacat ttaaaaggga ggataaatga atggactaca aaaaaattgc cgaagatgta
     1561 ttggaaaacg ttggaggaaa agagaatatc tcaagcgcta cacactgcat tactcgttta
     1621 cgtttaattc ttaaagacaa aagcaaagcg aatatggaaa tgcttgagga aatagaaggt
     1681 gcaaaaggtg ttcttttcaa tagtggacag ttacaagtaa ttttcggaac aggtataatt
     1741 gaaaaggttt acaaagagtt cgttgaattg acaggagcta aggaagtatc cgtttctgaa
     1801 atgaaagacg aaggagtcaa taattcagga aatagacttc aacgagcttt taaagttttc
     1861 tcggatatct ttattccgat cattccagct tttattgggg cagcaatggt tttaggaatt
     1921 cgttcgttga ttaccactcc tggaatgttt ggaatggatg gctcattcgc tgatcacaat
     1981 gtattcctaa atgatttatc caaatttctg ggtattattg caaccacatt tgattattta
     2041 ccagttttag ttatgtattc tgcaacaaaa cgttttggcg gaaaccctat tttaggatta
     2101 cttgtaggat ttgttatgat tcatccagga ctagccaatc ggaatgattt cgtcatggga
     2161 actataaagc ctgattattg gcatttattt ggattaagca ttccagcagt tgcgtttcaa
     2221 gggggtgttt ttccagcaat tttgacttct tggtttttag caaaagttga gaaaattaca
     2281 aataaatatt ctcctcaagt acttgcttac attttagtcc caactgtgac aattgttctt
     2341 gccaactttg cactattttt agtgtttggt ccattgggga attttatcgg taatgtttta
     2401 ggtggaatca ttgatttcct atatatgaag atgggtgcct ttggtgcttt tgtttttgca
     2461 ggcgcgttac aaccattagt tgtaacagga acacaacatg ctattcaagg tattgaggca
     2521 aacttagtcg caacaactgg attcaattat atccaaccta tttggtcagt ttcaattatt
     2581 gcccaaggtg gcggttgtat tggtatgtat ttactcttta agaaaaaatc taaagataga
     2641 gatattgcga tttcaagctt tattccaaca ctggtaggta ttactgaacc ggctatcttt
     2701 gctgttaatt tgaaatattc aatcgttcct tttgtttgct cgtttattgg tgctggtttt
     2761 ggtggagcat ttatgaaaat ttttgatgta aaagccattg gacaaggatt gactgtattg
     2821 ccaggtctga ctattgctaa tccagttctt ccttatatat ttggtaatct catagcattc
     2881 attttaccaa tcatttttat tctcgcttat gataaatata aagtgtttat ccctgaagct
     2941 aaacaccgta aatttgcaga aaaaactgac tcaaaaaaat tgccagttca agtaaagtga
     3001 gatgagagca tgttaaattt ttttagaaaa aatgaaataa gcgttgtagc acctgcaacc
     3061 ggtgtttttg aaaaactgga gaaattaagt gatccagtct tttcaaaagg aatgatggga
     3121 caaggatttg caattgatgc aaaaaacgaa acgattgtat ctccaatcga tggttttgtt
     3181 tcaagtattt ttcctacaaa acatgcctta ggaataaaaa caaaatcagg aattgagata
     3241 cttgttcatg taggaataga tacagttgag ctaaatggtg agggatttga tattaaagtt
     3301 caagaaggac aaaaagttaa ggctggggat attcttatga ccattaattt tgtagttttt
     3361 gaaaagaatc acaagactaa agatgtcatt gttgtttttc cagactacca agacgaaatt
     3421 ttcattccga aacttgaaga agtaaaacaa ggacaagaat tgtttgttct tggctaatga
     3481 taagtaatta ataatagaat cgaggaaaat gtatggtttt tggggctatt gaagctggtg
     3541 gaacaaaatt tgtttgtgcc attggagata aagatttgaa catatttgaa aaagtcagct
     3601 ttcccacgac tacacccaaa gaaacaatga atttagtttt agattttttt aatcatcata
     3661 aagatgattt agaaggtatc ggagtgggat cgttcggtcc tattgatatt cattgtgaat
     3721 ctaaaacgta tggcttcatt acatcaacgc caaaacttgc ttggcaggat tttgatttta
     3781 ttggaagttt aaaaaagcaa cttaatattc caactgcttg gacgactgat gtaaatgcct
     3841 cttgctatgg tgaatatgtt tctggaaatg gcagaggcct ttcaagtgtt gtttactata
     3901 ccgtaggaac gggcatagga ggtggcgcta tccaaaacgg aaacttcatt gaaggtttta
     3961 gtcatccaga aatgggacat atgcttgtaa aacgacacaa taaagacaac ttttctggga
     4021 catgtccatt ccacggagac tgtttagagg gcttagctgc tggtccggca atcgaaagaa
     4081 gatcagggaa aaagggacaa gaagtgactg aaaatgacgt tacttgggaa atagaagctt
     4141 attacattgc tcagtgtgca tataatacaa cattgatgtt atcacctgat gtcattatat
     4201 ttggtggcgg agtaatgaaa caaaaacact taaaaaagaa agtacaagat aagtttattg
     4261 agttgatcaa cggatatgtc gctattcccg atgttgacaa ttatattatg acaccaaaat
     4321 tgttagataa ttctggtatt gtaggttgtt tagctttagc aagagacgtt aaagaaaaat
     4381 cttaatttgg aaaaaagatc gtctcgacta tttgtttggt tgatgcgatc tttttttcaa
     4441 ttagtaattg aagggacgcc taaaagtgaa aaaaaaaatg aaagatgaag tattagagta
     4501 tatcaaaaat aatttaaagt attacgattt ttctgctcaa gatattgcaa tgaaattttg
     4561 tattaaaaga aatgtcgctt cccactattt gaatcagtta ttttcagatg gaaaattact
     4621 aaagaatgat tctgttagac cagtaatgtt caagtataat caacaaaagc caaaagactg
     4681 cttttctaag tttattggag cagacatatc tttgaaatcc actatagata aatgcaaagc
     4741 tactgtaatg tatcccccta atggcctccc attaataata aaaggaaaca gtggcgttgg
     4801 aaaaagtttt ttagcaagtt tgatctatca gtatgctctt gatagaaaag taattcataa
     4861 tgacgcaaaa tttgttgttg tgaattgtgc agattatgca aataatccag agctattgtc
     4921 agcagtttta tttggatata aaaaaggagc ctttactggg gctgaaaaag atactgcagg
     4981 gatgctatct tctgctaatg gaggatacct ttttttagat gaggttcaca atctaagtgc
     5041 agaaaaccaa gagaaattgt ttcttcttat ggattctcaa aagtatcgta aacttggtga
     5101 aagtgtgcaa tgggagtatg caaatgttcg ccttatctta gccacaacag aagataaaaa
     5161 tacctctttg ttagcaactt ttagaagacg catcccagct gaaatcactc tgccagatta
     5221 caataataga agtacatctg aaaaaattca attattattt gaatttctta aagatgaagc
     5281 aattacgatt gattcaaaaa tattttgttc gattgaactt tttactgatc ttttatcgaa
     5341 aacatttgaa ggaaatgttg gagaattgag gaatgaaatc aagcttcttt gtgcagaagg
     5401 ttatttagga aataagagaa agtcttcaat ttatttaggg gacaaactag acagcggttt
     5461 ttggatagat ccagaaatta gtatagattt aaaaaaaatg tttcttaaat cactttctga
     5521 aattgatttg attgaacttt ttcaaaatca catttcaatt gaagaaagca tgagtcaatt
     5581 aagagataga atacttaaag agtgtgcagg ttcttctgag tataattatt tagaaagtga
     5641 cgaatttata gcattaagaa actatgttgt taataaaata tcaccaatta ttgattcgac
     5701 aggtctatgt ttattagatg aattcttggc ggatatcagt ctatttataa tgtttattga
     5761 tgtaattgaa gaaacatcaa gaaatttttt gctgtctaag ttcagaaaat ttcgtatgaa
     5821 aaatgataaa caaaaattat tggcaaatga gatttggagt agtttagaag tcgaagaaag
     5881 taaacaagaa cttttgttga aatggatact ttttcttgta aagaagttct atgtgaaaat
     5941 accagaaacc cattgtctga tagttatgca cggtaaaata acagcttctg cgatcgcaag
     6001 ggaaacaaat aagttgctga atacatatgt ttatgaggct ttcgatatgc caattgaagg
     6061 tgagactagt gacttaatta acctaatcaa caatttttgt aaaagtatag atacgacgaa
     6121 tggactcatt ctgctagttg atatgggatc attggaacaa atgtatgaaa aaattgaatc
     6181 caatgtaata ggtgatttgg taattttaaa caatgtttct acagctttag cgattgaatg
     6241 tgggattcaa gtttgccaaa aaaagccaat tagtcatttt taccagatgg attttgattc
     6301 gtttcaagtt aaagttcagt attataaagg attatcacaa aagaaaaatg taatagtatc
     6361 ttgtctctct ggtgagggga tttctgaaaa agttaaggat attttaaaac ggtatctcga
     6421 taatcaagtg gagattttga tttttgattt taacgcatta aagaaaatag caaaggaa
//
LOCUS       DAZKBH010000126         6303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000126 DAZKBH010000000
VERSION     DAZKBH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.128"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACG8OO_002505"
     CDS             334..714
                     /locus_tag="ACG8OO_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9046346.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACG8OO_002506"
     CDS             696..1610
                     /locus_tag="ACG8OO_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9046347.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACG8OO_002507"
     CDS             1855..2544
                     /locus_tag="ACG8OO_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046348.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACG8OO_002508"
     CDS             complement(2665..2751)
                     /locus_tag="ACG8OO_002508"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9046349.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACG8OO_002509"
     CDS             3427..4377
                     /locus_tag="ACG8OO_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9046350.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACG8OO_002510"
     CDS             4398..4931
                     /locus_tag="ACG8OO_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9046351.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACG8OO_002511"
     CDS             4947..5117
                     /locus_tag="ACG8OO_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046352.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACG8OO_002512"
     CDS             5567..6100
                     /locus_tag="ACG8OO_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046353.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACG8OO_002513"
     CDS             6112..>6303
                     /locus_tag="ACG8OO_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046354.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2324 a         1102 c         1173 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtatacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAZKBH010000127         6287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000127 DAZKBH010000000
VERSION     DAZKBH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.129"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACG8OO_002514"
     CDS             190..690
                     /locus_tag="ACG8OO_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9046355.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACG8OO_002515"
     CDS             787..2634
                     /locus_tag="ACG8OO_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFP9046356.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ACG8OO_002516"
     CDS             2637..3533
                     /locus_tag="ACG8OO_002516"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9046357.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ACG8OO_002517"
     CDS             3583..3972
                     /locus_tag="ACG8OO_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9046358.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="ACG8OO_002518"
     CDS             3959..>6287
                     /locus_tag="ACG8OO_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9046359.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAZKBH010000128         6261 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000128 DAZKBH010000000
VERSION     DAZKBH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.130"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2452
                     /locus_tag="ACG8OO_002519"
     CDS             <1..2452
                     /locus_tag="ACG8OO_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFP9046360.1"
                     /translation="QKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQ
                     VERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDEV
                     DIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVEE
                     NSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPL
                     RKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQ
                     EYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEI
                     EIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKENEA
                     MIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASI
                     VLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIK
                     LASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLT
                     SSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKL
                     FEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEH
                     IQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSI
                     QKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQV
                     LPTYEILLPSSLKSYFRL"
     gene            2634..2873
                     /locus_tag="ACG8OO_002520"
     CDS             2634..2873
                     /locus_tag="ACG8OO_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046361.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2889..4262
                     /locus_tag="ACG8OO_002521"
     CDS             2889..4262
                     /locus_tag="ACG8OO_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9046362.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4266..5900
                     /locus_tag="ACG8OO_002522"
     CDS             4266..5900
                     /locus_tag="ACG8OO_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9046363.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6063..>6261
                     /locus_tag="ACG8OO_002523"
     CDS             6063..>6261
                     /locus_tag="ACG8OO_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046364.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2187 a         1025 c         1273 g         1776 t
ORIGIN      
        1 acaaaaaaag cattctgtga gagacaatga tattgaaaaa attcggtttg tatttattga
       61 tattgaccct aaaaggaaag agggaagtgc aacagatagc gaaaagaaaa aggcagaaaa
      121 tgttatggaa caagtagaac gttatttgaa agaaaagaga atagaatcat ttgtaaaagt
      181 tgatagtggg aatggctacc atatttttct tcccataaat gaacaaccaa ataatcacga
      241 gacgattctt actattcaga attttcttca attacttcac cgaagatttg gagtagaaga
      301 tgaagttgat attgatactt ctgtttataa tccttcaaga ttatgcaaat tagttggtac
      361 accagcggtc aaaggaaagt ccacaaaaga gcgtccgcat cgtttatcta gtttcctttc
      421 tattcccgat aacatagata gaagtaattg ttttaaaatt cttgaacaga tagttgaaga
      481 aaattccatg atgtcaacct ctagagtaaa aagccatcca tcttttagag aagtgttacc
      541 ttttattcaa gcagactata aacaatggtt agctcactat cctgaattaa cttataccat
      601 aaaagagggc gatcataaag gagttacctt acttatattt gacgaatgtc cattaagaaa
      661 acacacgaat aattctaatg gttcttgttt gtctgttacg gctgataata agattcgatt
      721 tcattgtctg catgcttcgg ataaaaataa agatattcaa gactttgtaa aaaaatatcc
      781 gttgccaaaa cctgtaaatc cttctgtaga atcattagaa tccaatcaag aatattattt
      841 tgaccagttt aaactgaatc aagaaggagt atttttggtg aaagaaggtc aagaagaaaa
      901 agttgcttgt ccgatgttta ttaagcaaat acgcaaaaat atcgaacaaa aagtcattac
      961 acttgttctt gcctattatg tcgatcattg ttgggatgaa atagaaatag aaggtggatg
     1021 tttacaaact aataatttta aacaattagc aaaacatggc ttaatcatta aagctagaag
     1081 agaagctgaa gtcgttgact ttttacagat tcaacgggag aatgctccaa tcttttttga
     1141 acatgaatct cttggctgga aaaaagaaaa tgaagcaatg atttttcagt tacaagattc
     1201 ttttggacgt gaaattcctt ctgtattaac taaaaactca tgctaccaat taacaactaa
     1261 tggtacagaa gcggaatttg acgcattggt tcataagtat gttttaggaa cgaacttaga
     1321 acttgcttgg tcgattggta ttgcttctat tgtgttaggc tatttaaacg ttacgaaggc
     1381 taatactttt ttgagtttac taattaacct acagggcaag agcacaacgg gtaaaaccac
     1441 tacgttacac tttattgcct ctttatttgg aaatccaact acggttctta gaaatatgaa
     1501 tgctaccaat aaagccatta taaagctagc atcaaacaat cagggaggtg ttccactaat
     1561 actagatgag ttaggagcag caactcaaaa taactttagc tcgttgatct atcaattagc
     1621 ttcaggagaa gaaaggctaa gattagattc taattgtcag cttatgccac aagagacttt
     1681 ttctgttttg acccttacct cttctgaaga gcgtttggaa aattatttca atgagaaatt
     1741 aactggtttg aaagttcgat atatcgaatt ttctgatatc aagtttacaa aggatgcgaa
     1801 tcacgcagag aaagttaaaa gtctttcttc taatactttt ggttggggta taaagttatt
     1861 tcttttgaaa ctctttgaac aagggattac aaaaattgaa gcaacttttg tcgaagctca
     1921 aaaaaaacta tgggctgttt tacctgaaca ttctttgaag caacgcattg gtaataatct
     1981 agctttgatt tatagtggag ccctcctaat tcaagatata ctgggctatc ctttacgatt
     2041 agagcatata cagacggtgc ttattaattc gtacgaacag ttaattgtag atactagttt
     2101 aggaagtgag caaacggtat ttgaccgagt gaaaacattg ttggtgatga atagccataa
     2161 atttttaaca aacagtgaca acaaacatac gcccagtacg atttggggaa aagtatcaat
     2221 tcaaaaaaat ggggatatta aggtaaatat ttttcctatc ccgtttgagg acttgttaag
     2281 gcgagaattt caagtaaagg atctaaccta tctttttagg caattgcttg attcgggtaa
     2341 tatgtgtgca gaaaaaggga gaagaacgaa acgtattcac atgaatcgtc aagttcttcc
     2401 aacatacgag attctattac cttcttcact caaatcgtat tttcgattgt aatttagaat
     2461 aaaccaaagt atgaaagtga ggcaccatgt tatggaaata tttcttattg atttagcaaa
     2521 acaactgatt acttatctgt atgatagcga gcaaacccaa acggaagtaa atgaagcttg
     2581 atctatgctt catttacttc attcaatcaa acatgaaaga aaggaaaatt ctaatgacta
     2641 aaaataaaat gacactaaaa gcagaggttc ttctgtatat tcaagaacat ttttcaaatc
     2701 aagccttttt tacaaaacca atttacttgg atttcgaaat aagaggggta tctgctggat
     2761 ctatcggggg tactcttcaa gcgttgaaga acgaaggata tttagaaaat cactttgtgc
     2821 aacgttcgtt caatagaaga gtcgtgaaaa aatggtattt agttcattca tagagagggg
     2881 aatcaagcat gcgtgtggct tgttatgatc gaacgtctgt ttatgaagaa gcgaaaatta
     2941 gtaatgacgc tcaatatgat gcagtgaaag agtatgtttt agagaaatat gaacaaattc
     3001 cccaacgatt ttcagacaaa ggagaatcag gggaaaaagc ggagcgtcca gcttttcgac
     3061 agcttagagc ttgggtttgt gcgtcagaag aagagggggt gatctatgtt tggcgttatg
     3121 atcgattggc acgtcaagcc aaaatagcct tagaatttat tgagttgtgc caacaacatc
     3181 aggttcaagt gtattcgatt tctgaacctc ttcctgcttc tttctcacac gcgtcaacga
     3241 ataaactatt tgtgcaatta ttgttcactt ttgcagaaca acaacgaaac gtcattattg
     3301 agaatgttcg tgccggactt tcctataaaa agcaacaacg gcaatatttg tcttctgcgg
     3361 taccatatgg gtatcgttta gtgaatggga aaatccaaca agaagtacac gaagcaagga
     3421 ttgttcgcta tatttttcaa ctttatttga ccaaaaaata tggctacaaa aagctttgtc
     3481 aacgattgac gcaacaaaag cttttcttcc gcgaacgtcc ttttcagccc tatcatattt
     3541 atagtatcct aaaaaatcca ttgtactatg gcgaaatcaa aggaggatct ttggggaagt
     3601 atctaggaac ctttgaaccg attctctcga aagcaacctt tttacaagcc caagagattc
     3661 gccaatcaag gtgtacagct aaaaaagata cgtatctgta cttattacgt caaaaaattc
     3721 ggtgtccctt ctgtggtcgt catctgagtt cgaagtatca atggaataca aagaagacga
     3781 agacgcttca ttattatcat tgtaccgacc gaagctgcca aggaattttt cttcgggcag
     3841 aaagggttga gtcagaagta ttggaaaaga tacaggcttt ttttcaatac gaaggcgtct
     3901 atcaagcctt gattgcccaa ttaacgcaac aattagccga aaagcgaaaa gatgaaaaac
     3961 gcaaagtaat ccaacaaaag aaacaagaag cacaatggat taaggcattt gaagagggca
     4021 attgttcact ggaagaactc aagcaacaac tgcaagctct tcaaaagccg atgtctactt
     4081 cttcgtttct ttcactccaa cagtgtgaag caaaagtgaa agaatggctt caactaaaag
     4141 acctctctag tcgacaatta ttatgtgact atgtttgtcg gattgaggta acaaaggata
     4201 aaacagtttg tgggatttac ttccgacaac tgacacaaaa tattttagaa aggaacgaat
     4261 gatctatgga aatgatacaa accaaacgag tcgcattgta tattcgcgtg agtacacaag
     4321 aacaagcttt agaaggacat agtattcaag cacaagaaca aaatggacga cagtttgccg
     4381 aacggatggg ctatgaagtg gtagaagttt atgccgatga aggaatcagt gggaaatcca
     4441 caaaaaatcg gttcgcctat caacgaatga tgaacgatgc tcggaaccat cgttttgatc
     4501 tactagtgat ttggaaattg acccgtctcg gacgcaatat gttagatgtc ttacaaacag
     4561 tcgaggaatt attgcgatat gacgtaggat tacattcgat ctcggaacaa tttgacgtga
     4621 ccacttcgac aggtaaattg atgttacaac tccttgggag tttcggtgaa tttgaacgca
     4681 accaaattgc ggagaatgtt caaatgtcaa tgcaaagctt agtgaaagat aaaaagcgtt
     4741 atgcaggagg ccgacgttta ggctatgtca gtggtattga cacgaatggt caaaaacaat
     4801 tggtcattga accagaagag gcgaaaattg ttcgtctgat ctatgcaaaa tatttaggcg
     4861 gagcagggta tcgagcgatt gccaattttt tgaatcgtca aggatatcaa acagtcaaaa
     4921 agaatgcttt ttctaccacg gcagtcaaag atatcttacg caataaaatt tatgcagggt
     4981 atttagaata tgcgcgctac atcaattggg aaaccaaacg acggaagggg aagaatccga
     5041 gaccgatttt agtgaaagga acccatgaac cgattatttc ggaagaagat tatcaaaaag
     5101 tacaagaacg ccttgcttta gaaggaaaac aacccaaatg gaaccataca ggtgaaaatg
     5161 tattgacagg cttgttacgt tgtccagaat gtggcgcacc gatggcagcg agtaatgtca
     5221 caaataccct aaaggatggc acaaagaaac ggattcgata ttattcttgt tcagtctttc
     5281 ggaataaagg agcaagtgtg tgtcatgcca attcgattcg tgccgaacaa gcggaaacat
     5341 ttgtcgccga ccgattaaag gagatcgttc agctaccaga agttcttccc cgattggtgg
     5401 cagcgttaaa tgaagaaata gtgcgacaaa gccaaccatt ggaacaagaa ctcgtggtgt
     5461 tactggaacg aaaggaagaa ttgaagacta aaattgaaaa atgggaagct gctttggaag
     5521 atagtcctga attgtttccg atgttaaaag accgcttaga cgaattgact gaaaaacgac
     5581 gacagttgca cattcgagaa aatgaaatct tagggatctt tcaacaagga gagccgattc
     5641 aagtcaaaga tgtccaacgg atattgacta atttggatcg ttttcttgcc cagagtgaga
     5701 agaagcaaat caaagccttg tatcgtacct ttatagagaa aataaccttt gatcctaacc
     5761 ataaggagat gttggaaatt acgatgaaat tcacgcatgc cgtggtacaa caattgaatg
     5821 agcaatatca aatagccgta tccaaaacaa aggatacggc tatttttgtg ttaaaaacac
     5881 cctttacgtt aacgatctag tctaggttat atcaaacaac gttagagggg cgctaacgct
     5941 tctctagcga tttgaaatcg ttagagaagt agttagggag gtccttatca taaataggga
     6001 tatttattaa tgaatatttg aaatataatt gtatataaga attaaaaaga gtggggatag
     6061 atatgaaaaa gatgggtaag tgtattttat gtgaaaaata cactgaactt actaaagaac
     6121 atgtaccacc taaaaaatca gggaatactg gaggtaaaaa gaccagaact ggtaatttag
     6181 atgatttcct taagagcgat tttacaaaag gtgattttcc gaaggggatt aaaaggaaac
     6241 ctcaaggaaa tgtttattat a
//
LOCUS       DAZKBH010000129         6173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000129 DAZKBH010000000
VERSION     DAZKBH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.131"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG8OO_002524"
     CDS             725..811
                     /locus_tag="ACG8OO_002524"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9046365.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG8OO_002525"
     CDS             complement(932..1621)
                     /locus_tag="ACG8OO_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046366.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACG8OO_002526"
     CDS             complement(1628..1906)
                     /locus_tag="ACG8OO_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046367.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACG8OO_002527"
     CDS             complement(1866..2780)
                     /locus_tag="ACG8OO_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9046368.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG8OO_002528"
     CDS             complement(2762..3142)
                     /locus_tag="ACG8OO_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9046369.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG8OO_002529"
     CDS             complement(3592..3879)
                     /locus_tag="ACG8OO_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9046370.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG8OO_002530"
     CDS             complement(3899..4123)
                     /locus_tag="ACG8OO_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP9046371.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACG8OO_002531"
     CDS             complement(4578..4748)
                     /locus_tag="ACG8OO_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046372.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG8OO_002532"
     CDS             complement(4763..5296)
                     /locus_tag="ACG8OO_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9046373.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG8OO_002533"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG8OO_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9046374.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZKBH010000130         6159 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000130 DAZKBH010000000
VERSION     DAZKBH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.132"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..2543
                     /locus_tag="ACG8OO_002534"
     CDS             102..2543
                     /locus_tag="ACG8OO_002534"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFP9046375.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="ACG8OO_002535"
     CDS             complement(2873..3577)
                     /locus_tag="ACG8OO_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046376.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="ACG8OO_002536"
     CDS             complement(3764..4009)
                     /locus_tag="ACG8OO_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046377.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="ACG8OO_002537"
     CDS             complement(4026..4877)
                     /locus_tag="ACG8OO_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9046378.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="ACG8OO_002538"
     CDS             5043..6047
                     /locus_tag="ACG8OO_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFP9046379.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1978 a         1065 c         1303 g         1813 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaa
//
LOCUS       DAZKBH010000131         6144 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000131 DAZKBH010000000
VERSION     DAZKBH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.133"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(246..758)
                     /locus_tag="ACG8OO_002539"
     CDS             complement(246..758)
                     /locus_tag="ACG8OO_002539"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9046380.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(772..3069)
                     /gene="recJ"
                     /locus_tag="ACG8OO_002540"
     CDS             complement(772..3069)
                     /gene="recJ"
                     /locus_tag="ACG8OO_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9046381.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3211..3654)
                     /locus_tag="ACG8OO_002541"
     CDS             complement(3211..3654)
                     /locus_tag="ACG8OO_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFP9046382.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3655..4443)
                     /locus_tag="ACG8OO_002542"
     CDS             complement(3655..4443)
                     /locus_tag="ACG8OO_002542"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9046383.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4440..5381)
                     /gene="rnz"
                     /locus_tag="ACG8OO_002543"
     CDS             complement(4440..5381)
                     /gene="rnz"
                     /locus_tag="ACG8OO_002543"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFP9046384.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5558..>6144)
                     /locus_tag="ACG8OO_002544"
     CDS             complement(5558..>6144)
                     /locus_tag="ACG8OO_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFP9046385.1"
                     /translation="SKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDPN
                     GTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEIK
                     DAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVHD
                     AKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
BASE COUNT         1665 a         1407 c         1055 g         2017 t
ORIGIN      
        1 tttacggggg tgtgacaaaa atcggtcaca tccttttttt cgaataaacg gtgttctaaa
       61 gccagttttt cggtattaag tcaaattgcg gaaaaagtga ttttttaaac ttctactggt
      121 ctttgcttgt ttccatttat aaaaaaatag cgaccctcaa aagtcagata gagaatctga
      181 cttttgaggg tcgctttaaa atcgcttctt taagaataac atcaagaata gcttcttttt
      241 tctgcttaat attccattaa tgtaatgatg tcatatcctt tgattttgtc gcgtccatgg
      301 agatccatca attcgatcaa aaatgcgcag cctacgacta caccgcctaa ttgttcgatc
      361 agttcgatcg tcgctttgat tgttcctcca gtagctagca aatcgtcaca aatcagcaca
      421 cgttgacctg gtttaatggc atccttatgc aatgtcaatg tatctgttcc atattctaat
      481 ccataagtca cttcgatggt ttctcgaggt aacttccctt ttttgcggac tggggcaaaa
      541 ccgactccta actcataagc aacaggacat cctacgatga atccacgggc ttctggccca
      601 actaccatat caattcgttt ttcttttgca taatttacga tttgtttagt agcttcccga
      661 taagcgtcac catctgccat cagtggggaa atatctcgaa acacaattcc ttttgacgga
      721 tagtcgggaa tactggcaat ataatctttt aaatccactc tttgtttcct cctaggttgt
      781 taaccatttc tttatggtag gaatatcgct caaaagcaaa aattcctcaa ctttgatttt
      841 attgagccgt cgctggtata atctgctctc agtcaaagga tgactttctg ggctagcgac
      901 tttttgtaaa acaccgttct ctattgtaac aaatttcaat tcaaaaaaca cctgtatcat
      961 aaaaattaat aatttttcag ggatacgcaa atattgagct attgctttta ttttatgacg
     1021 tatatcaatt cgttcttgag aatggatcaa tttaaacaat tttgcgtact gttctcttgt
     1081 gcctacccca ttcaaatagg cctcatcagg tgaaatcccc atcagataaa cacgattgaa
     1141 tgaataagac cgaaaaactt ctttcagatc cgctaaatca tctggacaat ccataacaac
     1201 tagagagttt ggttttcttt tttcaattat tgcctttaca gaatctagtc cttcgaaaac
     1261 aatcgcagct tcctggataa tctcaggcac gaatttcaac gtcgaagaat caaatgctaa
     1321 ataaaggcta ccttccgacg ttcggctctg ttcacggaaa cgttttgccc gccaatcaaa
     1381 tacttgcaga ccacccacag aaaaatcaga taccatcagc tgcggtttct tacgcccgtt
     1441 ccattcattg atcgaaagct ttcctaccac atctacctga ttattcagca gttcttcttc
     1501 ttgagtacca aaaccaaaag cgaccgcatc aagctgactc gatgcatcgg aaaggacaaa
     1561 tttcagatgc tgttggtttg ctccgattct tttgatattt tctgcttgga cttgtcgaaa
     1621 taaaaactga ggcaaaggat tatccgtacc aaatggcgcc aatagtttca gctcatcaat
     1681 tcgctctact gttacttcat ttggcagcaa tacttcatca attctcaact caggccctct
     1741 catcaagtca atttgatgat cgactatata ttggttcatc ttctcctgta gtatagtgac
     1801 attttcactt ggcatcgtta aaccgactgc tgcatgatgt cctccgaagt aagtaaataa
     1861 atctcgcatc tggtcaagca tttcaaaaag attcaaggct tcaatactac gtccagatcc
     1921 tttagctgat ccatcttctt ttaaagtaag gacaagcgtt ggtttacccg tttcattcat
     1981 tattttccca gcaacaatgc ccaaaacgcc ttcatgccat cctcggttta ccaatagatg
     2041 gatatgattc tccttattga ccatcgctaa tgcttcttgt gttatctgct ctacgattgc
     2101 ttttctttct tcattgatct cattcaattt ttttgcttga acggatgctt cctcttcatc
     2161 aaacgtcgca agtaacgaga cggcaggacg tggatcgccc atccgaccga ttgcatttag
     2221 tctgggagca atcgaaaaac cgatcgttgt ttcgtctgcg tccctcattt ttacgccgct
     2281 ttcttcaaat agcgcctgaa gtcctatccg ttctgaatga tgaatggcat cgattcctaa
     2341 tgaaacaagg attcgattct catcagtcaa tgaaactaaa tcggcgatcg ttcctatcgc
     2401 tactaaatcc aaaaattctg caggcggttc ttccaataat gcagaagcta ctttgaatgc
     2461 tactccgact cctgccaaat ctggaaatgg atagtgcccc gctggatgac gagggtgaac
     2521 aatcgcataa gcgtctggta aattctcagg tagttcgtga tgatctgtaa tgatcacgtc
     2581 tacaccagct tgctgagcgt atgctaccgc atcatttcct gctaccccgt tatcaacagt
     2641 gatgatcaac tggattcctt cttcgatctt ttcttggtat accgattggt ttggtccata
     2701 cccatgctca aatcgatttg gcaggaatac ttctacttct gcaccaagta attccagtgt
     2761 ttctttcatt accgttgcac tggtgatccc atctgcgtca taatcaccgt aaacgaggat
     2821 tttttctcca ctgattacag cttcttggat tcggctgatt gctttatcca tatcgtgtat
     2881 cagatacgga tcatgtaatt cttgttcctt tggatgtaaa aatcggttga tatccttttc
     2941 ctcatggtaa cctctttgcc acagtaactt accgacaaat ggagataatt tcagttcacg
     3001 gatcatttgg agaaaggatt cagaaggctc tgtctcgctt aacttccaat caaaatttgc
     3061 ttgtctcact ttctcactcc taacttagtt attatagcaa aggaaatcag caaaaaaaag
     3121 cagaaagaaa gaataaaaac agatcagatc cgctagacag atgttcagca ggacctgatc
     3181 ttttgtacga ccatttatga aatatcccta ttagtcaaaa taattgaagc tttgtttggg
     3241 cgattgagtt ttttgttctt ccggcgcttt caattgactg atttgtgcgg ttttcgcttg
     3301 taactccgcc tgataagcag cttccaactc cgcacgttcg tttctaaact cgcgttgctg
     3361 ttcttctttt gcttcttcaa ttttcttttg aagttctgtt tgatattctg acatttcctg
     3421 agtcagctgt ttgatttgct ttttttgctg ccatagtgtt gccgaagcag tcaaaaatac
     3481 aatcagtgca ccaatcagcg cagaaccgat gatcactaag atcaatggcg cagaaaagga
     3541 agtgaagcca aaacttacag gaacattttt attgttcaat acagcaaaaa gaacaataat
     3601 caaaactaaa acaaatccaa gaatcacatt gctttgcttt ttcaattcct tcacctactt
     3661 tttgttaaaa attccgctgg ctaaataatc accaatcgtt ggaaacagcg tataaaattt
     3721 tgatgcaact tctaaaataa acggttgatt gatttcccgt tttggatgtc tcatattgcg
     3781 gacgatcatt tttgccaact tggcaggttc taaaacgatt tgtccgactt tttccaaata
     3841 gcttccgctt ggatcagcct tatcgaagaa atttgtctcg attggacctg gattcactgt
     3901 cgtaacagct acgccaagtg gtttcaattc taatcgcaaa gcgttggaga agccaaggac
     3961 tgcaaatttc gtggcagaat aaaccgttga ttttgcagtt gccatttttc cagccataga
     4021 ggcgatatta atgatatgac cttgtcttct ttctgccata tcgatcgcaa atttttgtgt
     4081 caaaaccatc attcctaaga tgttgacttc aaacatatcg tatgcttttc caaggtcgaa
     4141 tgtaaggaag tcttcgaaaa tcccaaaacc cgcattgttt accagaatat cgacgcgccc
     4201 gacttcttcc atcacctttt catataattc gtccacgctt tcaggattgc tgacgtctac
     4261 cttgaaagca taagcctctt tgccgctcaa gcgtctgcac tcgtctctga caccttctac
     4321 aaatgccatt cttcgtgcgc aacttataac gattgctcct tgctttgctg cttcataaca
     4381 gatttgtgcg cccagaccgc ctgaactacc tgttactaaa acaactttat cttctaaatt
     4441 catgcctctt cctcctcgcg aaatggaatc tcaatgctat caaaatcttt cataatcgtc
     4501 gtgttttcaa acacttcttg tgcttctttt tcaagctcta aagctgcttt tcctaaatat
     4561 ctcgcactga tatgagtaag tagcagccgt tttgcctgcg cttcttttgc aacttcagca
     4621 gcttgatgag tcgtggaatg gaaatagttg tgcgccattc ttgcttcatc tttattaaat
     4681 gtgctttcat gtaccagaat atcgctattt tccgcaagca caacggaatt atgtgttttt
     4741 ctcgtatcgc ctaaaatagt cacgattcgt ccttttttat ctggacctac gaaatctttt
     4801 ccattgatcg tccgcccatc ttctagttgg atcgtttcac cttgcttcag tttgccgtac
     4861 agtggtccag ctggaatacc taattctttt agacgatcca cctgcaattc tcctttatga
     4921 tcatgttcaa ccacacgata accaaaactg tcgatcccat gatttagttt ttttgtataa
     4981 acagaaaatt gctgatcagt aaaaatcggg gctgtttcat ttagctcaat gaatttcagc
     5041 ggataactta aacgcgtttg cgaaatacct aaagagactt ttatgtattc ctctatccct
     5101 tttggcccgt aaatctctaa tggcgtatcc ccaccttgaa atgaccggct gctgatgagc
     5161 ccgggaagtc caaaaatatg atcgccgtgt aaatgtgtaa tgaaaatttt accgattttt
     5221 ctcggacgaa tagtcgttcg caaaatctgc atttgcgtgc cttcgccaca gtcgaacaac
     5281 caaacttcat tgcgctcgtc taaaagtttc aatgctatac ttgtcacatt tctatgtttt
     5341 gcaggaacac ctgcacctgt tcctaaaaat tgtaattcca taatctacct gactttcttt
     5401 aagttcctct ttaattttag ggtaaaaaga agggataatc aaccgtaagt cgagaatggg
     5461 ttcaaattaa aaataacgtt ctaaagcgat agcgttttct ttgaaaatta ctataaaaac
     5521 agcagcgaac ccgtttgaga tcctctgctg ttttttatta atttgaagat ccagaacttt
     5581 cattgtcgtt ggaagtttgc ggatgtttgt ccttccaagt cttttgtgca gcttctttcg
     5641 catgattttc agcttctttc gcatcatgca catctttttc gacatcttca taatccaagc
     5701 gagtaatttc tccgcctaat tccatcatta ccaattgatt cagtgggcga ttgtcctctt
     5761 catcagcaat caagatcagt cctgtagctc cttcaccgat tttatttcct acaaagtcga
     5821 acacggtctg ggcatctttt atttctttgg catcttgtgt cgcaccgatc attcctccgg
     5881 caaaccatcc taataaaatc cctagcggac cacctaatac accaaccaac attccgatta
     5941 atccacccgt agaagatttg ttattaccgg taaagtcgat aaaatcttct attttgaatt
     6001 gatgtgtccc attaggatca tggctgacaa ctgccatctg ttctccttta atcgaacgaa
     6061 cagcatgtaa ctttttgatt tctgaaaatg cctgatatgc ctggctttct acttgaaaat
     6121 ttaaaatgat tactcgtttg ctca
//
LOCUS       DAZKBH010000132         5872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000132 DAZKBH010000000
VERSION     DAZKBH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.134"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(169..3318)
                     /locus_tag="ACG8OO_002545"
     CDS             complement(169..3318)
                     /locus_tag="ACG8OO_002545"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP9046386.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3332..4549)
                     /locus_tag="ACG8OO_002546"
     CDS             complement(3332..4549)
                     /locus_tag="ACG8OO_002546"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9046387.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4539..>5872)
                     /locus_tag="ACG8OO_002547"
     CDS             complement(4539..>5872)
                     /locus_tag="ACG8OO_002547"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFP9046388.1"
                     /translation="GYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGL
                     FDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAG
                     KKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKY
                     HGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSA
                     NWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAA
                     EGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKG
                     KNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPI
                     DMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1609 a         1247 c          979 g         2037 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag gtggaggaaa cattattcaa tagctgtctt
       61 aaatacttcg ttataaaatg tgaacaaata aaagacatct atccctattt tctacacttc
      121 cacttgacta ccatctttga atcgtacaat tttcttaact cttctcgttt acctcaattc
      181 attctcaagt ggaatcaact catcatctaa cattttacgc catgctcgct tcatttcttg
      241 tgggtattta aatggcttcg cttctggatg tttggcttta tatccttcgt aatccttact
      301 gttgataatt gctttcatat taggaattgg atcttcacct ggtagatatt gcagattaga
      361 agcatgtaat tctttctcag aaacaaacca ctcattggaa aagacatgaa tcaaatcatc
      421 actcgcttgt gtaaaatagc gacgtttaat gacaaagata tcttcattac tatctaaagt
      481 ccccacatca aatgcattta aaatttgtcg gtaaacttct ttgactcctt ctgttttatt
      541 caatttagcc aaagcttttt caatttcttc tcgtgcatct gaactattcg cagcataagt
      601 tcctagtaat tgatcaatat aagttgaatc aatatcatac aacttagtgg tattttcact
      661 ataaaattcg atcgttgaaa aatcttctcc ttctttttct ggttcttcct ctattaatga
      721 acccttcacg gtctcgtaca cgccaatttg ttcttccaaa agttgaattt gattgttcaa
      781 agttggagat ttcaccatgt catcattgta atcgtcataa gtcactaacg attcataagc
      841 acgattcaat tcttgaaatg ctttgacgta ctcgatacgg atttcaagtg gcgtatgttc
      901 atcaatggat tccatggatg aaacctcttt taacttacca tatgcttgtt tgaatctttt
      961 tttggattcc tgataacttg gataaatcaa acctgattct tcttttgctt ttgaatacag
     1021 ttttgtcgca tcctcgacat tcttctccat tgtcgctggc ttacgaaacg tcacaatcaa
     1081 tccttttgtc ttatcaggat acgtacgatt cgtacgtgaa aaagcttgga tcaatcctgc
     1141 atactctaga ttacgatcca caaatagggt ttgaatcgta ggggcgtcaa aacctgttaa
     1201 tagacgatcc actacaatcg ctaaatcgat ttgtttccca aattgtttga attccgctct
     1261 ttttcgtgcc aaacgattat tgatgtctcc gttataacgc tcaatgtctg ctaatgacca
     1321 ggaagtatca taatactgat tatattcctc aataattttt tgcatttctt cttgttgggc
     1381 acttgaatct cggctatttt cttctaatga ataggtaata gcgattcgtg gaaaatcagg
     1441 atcctccatc gttcgtcctt cacgaatgag ctgattggga aactcttttg ttaaccaatc
     1501 aggctctttc gtcatctctt tgatggcccg ataatacttt ttggccatgt caatggaact
     1561 cgtggttaag attgcagatt tcgttggcct gccatttcga aaatcaaatt ttgtataggc
     1621 attatctgga cggaacattt tatgaatgac tttttggata tgttcatctg cttcaaaaac
     1681 agctgggtcc aaataacttt cttttttgac aggttccatt tgatcaatca tacgattgat
     1741 ctgttctgat gaatactctg catatgtttc tacttctttt aatttccgat agattttatt
     1801 ctctagtgaa gtcggctcca cggtgttttc atgctctact tgaaatccta agacagagcc
     1861 atcttctagc gcattcttaa tggtataggt atgcaagacc tctccatact gatcatgggt
     1921 tgtacgtgcc aattgccctt tggcttgttt ccgattctca ggaaaaatcg gtgtacctgt
     1981 aaagccaaac caagtggatt tcggaaacat ttttttgatt tcttccatat tttcagcact
     2041 taacgctcga tgacactcat caactacaaa cacgatatgt tgccccatta atttttggaa
     2101 gcgattggtt ccttttttct cttcctgttt ctttgcatat ttcaacgccg catctagttt
     2161 ttgacgggtt gtaatgatca caacattcgc attggcatct gccaacagag tttcacttaa
     2221 ctctttggca cttcctgttc ccacgatcaa gctattcgct ttggcattac cagaagaaac
     2281 cccagtatta aactccgaag caaatttcgt aaactctgtg gtggtctgat tatctaaatc
     2341 tttgcggtcc actaacatga tcgttcgatc gatccccgat tttcgtgcta aaagcttagt
     2401 agatacaaaa ctagtcaacg tctttcctga accagtcgca tgccacacat accctgattg
     2461 atgtttattt gcggcaataa acaaggcttg aatcgcatgc acttgataag gatgtaacac
     2521 catgagggtt ttattgtctt gatcttcact gacaatcgta taatccgcaa tcaagcgatg
     2581 ggcatctggg atatttagca cttgtttgca aaactcatat aagttttcta cctttttatt
     2641 atctttcgtt cgccaactaa ataaaaactt aggatgcaaa tccttcggta aggcatttgc
     2701 aaaataccga gtcgtttgtt cattggaaac cacaaacaac tgtaaggtcg aaaagatatt
     2761 gttccgaaac atgccttctt cagcatattt tttgatttga ttaaacgcct gataaaaacc
     2821 atcttttgca gttacttgtt ttagctcgat ttggataatc ggtaagccat tgatcaataa
     2881 ggtgacgtca aaacgtcgat cccgcgcttc tatgttactt ccacgttttg caatttgatg
     2941 gaccacttca taagtagaga ttcctccacc aatgtcttga ttggaataaa ggataagtga
     3001 gacagaaccc aattgcggat tttcccgttc aatcgtgatc cgagccatcc cattttcacc
     3061 ttttaaccat ttcgcagcat caaatggggt tttcgtccgt agcaataatt cggttttgat
     3121 cgtttcaaac tctttatcgg tcaaaggaga atcattcagt tctgcttgat tatttgagat
     3181 gattttttga cgtaaattgc tccaaagatc ttcttctgat tttagatccg gacgatacgt
     3241 ccactgatta tgtccttccc cgaggacacg aatcaactgt tcttcgactt ccgcttcctc
     3301 acggtgacta attttcattc ttcttccctc cctaaacaaa catcttttgc aagaagcctt
     3361 ttttcgtttc cttcagcaaa tctaacttac gttgatgaag agcgatcgta tcatctagct
     3421 gcttgaagaa ggcaccaatt ttttgttgtt catttatatc cggtggaaac tgaaatgtcc
     3481 tttgttctat accaccttta gtcacatgta ccattgtcgt tccgttagtt gattctttaa
     3541 ttttctcttt atcagtcaat agccaagtat atagatactg tttgtccaat tccttagata
     3601 aaaatttaag tttccaaata tgataatggt agattacacg ttcttcattc cacatttcag
     3661 gaccgaagct tgttgcccat aaatataaga gatctccttt attagcatac ttgtcttcct
     3721 ctaattctaa gtttgaataa taccatctat cattagtatt aaaattacct actcttaaaa
     3781 ccctatattt tccagaatca agtaattctt gttgtttaaa agctctacca ttcaaaaaaa
     3841 ctaccacttc ccccaacttc cgctcttccc aatctcccgt aaatcctgga aaacgaactt
     3901 ctggcacttt tgctccgttt ttagggaaca ttttttgtaa gaatcctttt ttcgtttcct
     3961 tcagcaaatc taattcgtgc tggtgaagca tgatggtgtg atcaagttgt ttgaaaaatg
     4021 aaccgatctt gatctgttcg tcataagttg gaagtgagat aggtgtcttt ttaccactat
     4081 caattgtata ggttccgacg gttccaatgc ctgcaacgtc acggatatac ttctgaattt
     4141 tgttatttat gaacgagtag taaaagaagt ttccgtcaat tttatgctca ttcttgaacc
     4201 aaatgatgtt tccatcctta aagtaaattg ggttatcatc agtgattaat aaggggacac
     4261 ctattgatcc gacaccagtt acaagcaagt ctccttgaga aactttacct gaatttttcg
     4321 aatattcatt atatttttct tctgaaatgt aaagataatc ggatggttcc tcattttttg
     4381 cagctgagac aatatcacga gcacgtaaaa atctaatacc tgagttagtc caatctgatt
     4441 gatgaattcg cttcacagaa gtaacatcca tcatatctcc taacttacgc tcttcccaat
     4501 cttccgtaaa accaggaaac cggatttctg gtcgtgtatc attgctcatg tttgaacacc
     4561 tccagcgcac cttgtaacca cgcttcattt tcaggtgttg tttgcaggga agagatagct
     4621 tctaaaagtt ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg
     4681 acatgaacca tatcaattgg ttcttcttct tcgaaagtat cgacatagcg tgggatattt
     4741 aagttatagt cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca
     4801 tcttttcttt cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga taatttattc
     4861 tgatttttcc ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga
     4921 cgattcttct tcaatacaat caccgtcgtt ggaatcgatg taccaaagaa caagttcgca
     4981 ggcataccga tcacagcata aatactgccg tcttctaata atttttgacg aatgacccct
     5041 tctgcagcac cacggaataa aaccccgtgt ggcaagacga tagccatagt ccctgtttcc
     5101 ttcaaatgat agaaaccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat
     5161 ttcccgtagc gattaaaccg agaatcatct aagaaggtcg tatctgctga ccaattagcc
     5221 gaataaggtg gattcatcac tactgcatca aatgtgtaag gttcatctgt tggccagtcc
     5281 ttattcaacg tatccccatt gcggagattc atttcttcgg catctacccc atgtaaaatc
     5341 aagttcattt ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg
     5401 ttatctggat gagttaagta gttacgtaca tttaacatca aggaacctga acccatggtt
     5461 gggtcaaaga cactaaagaa tcgtcgttct ttttgatcca acgtcacgat ttgtgccatc
     5521 atatcagaaa ccatatgagg cgtgtaaaat tctccggctt tcttccctgc ttctgaagca
     5581 aattgactga tcagaaattc atacgcatcc ccgatcacat ccccatcgtg acctaaaact
     5641 tccacgtcat tcaatttctt gataacttcg gtaatcgtca catttcgttg ttgctcatct
     5701 gttcctagtt tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag
     5761 gtagatgcca attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt
     5821 gcttgatccg ctaagacgtt gaaaagatac tctggtgaga tcgaataccc ca
//
LOCUS       DAZKBH010000133         5809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000133 DAZKBH010000000
VERSION     DAZKBH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.135"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            450..1604
                     /locus_tag="ACG8OO_002548"
     CDS             450..1604
                     /locus_tag="ACG8OO_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFP9046389.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1577..2020
                     /locus_tag="ACG8OO_002549"
     CDS             1577..2020
                     /locus_tag="ACG8OO_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046390.1"
                     /translation="MGQYHYKLVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="ACG8OO_002550"
     CDS             3143..4075
                     /locus_tag="ACG8OO_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9046391.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="ACG8OO_002551"
     CDS             4092..4607
                     /locus_tag="ACG8OO_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9046392.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="ACG8OO_002552"
     CDS             4903..5184
                     /locus_tag="ACG8OO_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046393.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="ACG8OO_002553"
     CDS             5186..5602
                     /locus_tag="ACG8OO_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046394.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DAZKBH010000134         5790 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000134 DAZKBH010000000
VERSION     DAZKBH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.136"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8OO_002554"
     CDS             complement(99..1229)
                     /locus_tag="ACG8OO_002554"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9046395.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ACG8OO_002555"
     CDS             complement(1877..3079)
                     /locus_tag="ACG8OO_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9046396.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ACG8OO_002556"
     CDS             complement(3139..3342)
                     /locus_tag="ACG8OO_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9046397.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4551)
                     /locus_tag="ACG8OO_002557"
     CDS             complement(3484..4551)
                     /locus_tag="ACG8OO_002557"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9046398.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4609..4716)
                     /locus_tag="ACG8OO_002558"
     CDS             complement(4609..4716)
                     /locus_tag="ACG8OO_002558"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9046399.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4879..5691
                     /locus_tag="ACG8OO_002559"
     CDS             4879..5691
                     /locus_tag="ACG8OO_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9046400.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAZKBH010000135         5760 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000135 DAZKBH010000000
VERSION     DAZKBH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.137"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..308
                     /locus_tag="ACG8OO_002560"
     CDS             <1..308
                     /locus_tag="ACG8OO_002560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046401.1"
                     /translation="IYTKYFTLSTKVSSINTCSSYVQLRKSRYRGTFFFAAFFGLMRS
                     TFAGRIRYKTSFFTKDINRVKETSFCCQFFHFSKNENKKFWKWRRILLAILCLVEKR"
     gene            391..1902
                     /gene="gadC"
                     /locus_tag="ACG8OO_002561"
     CDS             391..1902
                     /gene="gadC"
                     /locus_tag="ACG8OO_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFP9046402.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1915..3315
                     /locus_tag="ACG8OO_002562"
     CDS             1915..3315
                     /locus_tag="ACG8OO_002562"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP9046403.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3480..4920)
                     /locus_tag="ACG8OO_002563"
                     /pseudo
     CDS             complement(3480..4920)
                     /locus_tag="ACG8OO_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            5313..5760
                     /locus_tag="ACG8OO_002564"
                     /pseudo
     CDS             5313..5760
                     /locus_tag="ACG8OO_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
BASE COUNT         1848 a          926 c         1088 g         1898 t
ORIGIN      
        1 ctatttacac aaaatatttt acactctcaa caaaagtatc ttcgattaat acttgctcaa
       61 gttatgtgca gctacgaaaa agtaggtacc gaggtacttt ctttttcgca gctttttttg
      121 gacttatgag aagtacattc gctggaagaa tacgatataa aacgagcttt ttcactaaag
      181 atattaatag agtgaaagaa acaagctttt gttgtcagtt ttttcacttt tctaaaaatg
      241 aaaataaaaa attctggaag tggagaagaa ttttgcttgc tatactttgt cttgtagaaa
      301 agaggtagac aaaataatgc aagctcaaat aagcgatttt ccataacctt ggtaggaatg
      361 acaaattaaa taggaaaggg aagtaataat atgaataaaa aaaagttatc tctattcggt
      421 ttttttgccc tgacagcttc catggttttg actgtttatg agtatccgac ctttgcaaca
      481 tcacaattac atttggtttt ctttcttctt ttgggaggta ttttatggtt tctaccagtt
      541 gctttatgtg ctgcagaaat ggcaacagtt gaaggatgga aagatggtgg gattttcagt
      601 tgggttagtc aaactttagg agaacgtttt ggttttgcag ctattttttt ccaatggttt
      661 caaattacag taggttttat tacaatgatc tacttcatac taggagcgct gtcgtatgta
      721 attaattttc cagagcttaa tacagatcca tttatcaagt ttattggtgt attagtcatt
      781 ttctgggtac tgactttttc acagttgggc gggaccaatc gtaccgctaa aattgctaaa
      841 gcaggatttg tcattggaat tgttattcct tcgatcattc tctttggttt agcagcagcc
      901 tactttatcg gcggtaatcc gattcaaatg gctattaatg cgaaagcttt tattcctgac
      961 ttttctcagc tttcaacttt agtagtattt gtatctttta tattggctta tatgggcgta
     1021 gaagcttcag catcacatat taatgagctg gaaaatcccc aaagaaacta cccattggca
     1081 atgtttttac tagtgatttt ggccatcgcc ttagatgcaa tcggcggttt ctcagtagca
     1141 gcggttattc cacaacatga cttgtctctc agtgctggtg taatccaaac atttgaaacg
     1201 cttattttac attttaacag tcatttacat tggttggtaa aagtgattgc tctcatgatt
     1261 gcctgtggcg ttatgggtga agttagttct tgggtagttg gtccatcacg agggatgttt
     1321 gcagctgctc agcgaggtct gttaccagtt ttcttccgga aaactaataa acaagacgtt
     1381 ccagttccgt tgattatgat acaaggaatt atcgttacta tttgggcagc agtcttaact
     1441 tttggtggag gaggcaacaa tttatcattc ttagtagcaa tctccttaac tgttgtcatt
     1501 tatctcgtag gatatttact atttttccta ggttactttg tattagtttt taagaaaaaa
     1561 gacttaaaac gaacttacaa tgtaccagga aaaactgttg gcaaaacgat tatcggtgga
     1621 attggtttta ttttatcagt gttcgcattg cttatctctt ttgtaccacc agcttcaatt
     1681 gcagctagtg agcaccgaac ataccaaatc atactactta ttagctttgt agtgactgtt
     1741 cttttacctt ttatcattta tgaagtacat gataaaagtg ctcacacaac tattgaggaa
     1801 cctactcacg tgaaagctga agacgttaat cccgctgttt atccagctgc tagaggagag
     1861 caccacgtta caaaaaatga tgaacatatt ttaaaacatt aggaggaatt cattatgtta
     1921 tacggaaaag ataatcaaga agaaaaaaac tatttggaac caatttttgg ctctgcaagt
     1981 gaggatgttg acttgcctaa atataagtta aacaaagaat ccattgaacc acgaattgct
     2041 tatcaattag tacaagacga gatgttggat gaaggaaatg cgcgattaaa cttagctact
     2101 ttttgtcaaa cgtatatgga acctgaagca gtgaaattga tgacccaaac gttagaaaaa
     2161 aatgcaattg ataaatcaga atacccacga acaacggaaa ttgaaaaccg ctgtgtaaat
     2221 atgattgctg atttatggca tgctccaaat aatgaaaaat tcatgggaac ttcaacgatc
     2281 ggctcttcag aagcctgcat gctgggtggt atggccatga aatttgcttg gcgtaaacgt
     2341 gctgaaaaat taggtcttga tattcaagca aaaaaaccta acctggtgat ctcttctggt
     2401 taccaagttt gttgggaaaa attctgtgta tattgggatg tggaactgag agaagtccca
     2461 atggatgaaa aacatatgtc aattaatcta gatactgtca tggattatgt ggatgagtac
     2521 acaattggta ttgtaggtat tatgggtatt acttacactg gtcgttatga tgatatcaag
     2581 ggtctgaatg atttagttga agctcacaat aaacaaactg actataaagt atacattcat
     2641 gttgacgctg catcgggtgg cttttatgca ccatttactg aacctgatct agtttgggat
     2701 tttcaattga aaaatgttat ctcaattaat tcttcaggtc acaaatatgg tttggtatat
     2761 ccaggtgtgg gttgggtctt atggcgtgac caacaatact taccagaaga attagtattt
     2821 aaagttagtt acttaggtgg agaaatgcca actatggcta tcaacttctc tcatagtgca
     2881 gcacaactaa ttgggcaata ctacaacttt gtacgctatg gctttgatgg ttatcgtgat
     2941 attcaccaaa gaactcatga tgttgctgtt tatttagcca aagagatcga aaaaactggt
     3001 atttttgaaa tcattaatga tggatcagaa ttgccagttg tgtgctataa gctgaaagaa
     3061 gatcccaatc gcgaatggac actatatgat ttatctgatc gtctgttaat gaagggatgg
     3121 caagtcccag cctacccatt acctaaagac ttggatcaat taattattca acgcttagtt
     3181 gttcgagcag actttggaat gaacatggct ggtgattatg tacaagatat gaaccaagca
     3241 attgaagagt tgaataaagc tcatattgtt tatcataaaa aacaggatgt gaaaacgtac
     3301 ggctttactc actaaaattc ttcagagaaa tataaaagaa gttaattaac taataaaagg
     3361 agttgtgatt accacacagc tccttaaatc tatttgaata aatatatgat attcatttgt
     3421 tacactctca atcttagctg ttgttctatt catcattact cttattgtaa gtagttcagt
     3481 caaatttgct tgttcaattg atcgataaca gtatgaattg tttgaaaagt aatcggatat
     3541 ataattgttt tctcactacc taagccttct tggtataaag aactatcgca aagtataatc
     3601 cgctgagtac aaaagaaagt gtcgtctact atttctctaa gttgtttaca aatgatatta
     3661 ctaatagaaa agtgttctcc caaaatgtct tctaactgtt gtcgatagta taaataccta
     3721 aagtctgacg gagctactat gaacacttca attggagatt tccgtttatg ctcatttaaa
     3781 agacttaaac taatggttag tttacgaata gaattctcat tccaacgcag aaataaattg
     3841 ttttgttttc gccattgttc aagaatttca tgaacttcta agtacaaagc gcgttgctct
     3901 tcatttaaca agtaagtctt atcaggttga aatatctgaa tatcagccca aagtgagcgg
     3961 ataaacggga gaagtgcttt atcaaaaagt atctgatttt cttcagatat attcagagat
     4021 aagataagtt tttgactgag ctcaatcacc aaagggttat ctgctacatg gttttgataa
     4081 acaacgttca agtctttttg taacaattca aagttatcgc atagataatt tctagagttg
     4141 aatagaaagt agatataaag gcattcatct tcgtcatgac ataaagagaa agaaagttgc
     4201 tctaatcctt tctgtattag ctgaaacaag ggagtatgtc gggcaactct tttttcttcc
     4261 tgactaaaat atattgggtt agtcttagat ctctgcaaac cgatagcgat acctaataaa
     4321 ataaattgtt tttcaactgt agggtaactt cttgaaaaaa aatgtttttc tacataatca
     4381 atcaattgtt gaccgctttg aataattgat ttatttagct ggatcaactc tgtccgattt
     4441 gtctcataga ttatcgttag taacaaactt cgataatcct tttcaggaat ttcaaaatgg
     4501 ccatcagcat ttaagtactg catttgttta aaaaaattaa tcaactcatt tcgtaagttg
     4561 taaactttgc ttaacgaaat gaaggtagta tcagctatat tactccaatt gtgttcccct
     4621 gataaataat gtcctagtac attgaaaaag tcagagcgct gatagatgag ttgagtgagt
     4681 gccaacctcg aaaacgaaga gtcgaatgcg actgtaaagc tacttctaga tttctccact
     4741 gaaataattt gctgatggag tatgtcggta atgcattcca agtcgttagt gacagacagt
     4801 gagtgggcaa acgttcaatt gatctgctat ttcgcgaatc tgaatggtcc ctttttcgtg
     4861 taattgctca caaatgtaaa cttgacgaaa aatacttttc tccagatagg tttccaacat
     4921 agtactcctc ctagtcattt tatacataac tattataact ctaccaatcg gaatagtcct
     4981 gtaatatatt gaatcaaaaa tataaattat aaaataatta gatggggtat ttggtttaag
     5041 tatcccttat atagtaataa atttataggt gtgattctcg aattaatacc cgaaaatgtg
     5101 agagaatttt tatggatttt ttaggataca aattaattat ttttgctttt atcctgatta
     5161 ccagtccagt actgtcggta tcccattttg cagcaaaatc atgttgataa agtgacaaga
     5221 agactgatga gatatattat ccaaattgag aagataatta atcggattct tgagtatacc
     5281 ctacttttta agaaagtagg gtatacaagc gtatgaataa agacataact ttccctgtga
     5341 tggacgttcg gcttgttcca attaccaaaa tacaggcgaa cgattataat cccaatcgag
     5401 tagcgtctcc ggaaatgaac ttattggagc tttctatcat tgaagatggc tttactcaac
     5461 cattagtttg ttattacaag gaaaaagaag agaagtatat cttggttgat gtgcttcata
     5521 ggtatcgtgt tgctaaggaa agactcaact taaaagttgt accggtaaca gttattgata
     5581 aacccatata agaacgtatg gcttcaacaa ttcgacacaa tcgtgcaaga gggacccatg
     5641 aaataagagg aatggaaatt gtcatccaaa gattagtaaa aaatggatgg tcagacaaga
     5701 gaatatccaa agaattaggc atggacattg aagaaatttt tcgctttaag caagtgtctg
//
LOCUS       DAZKBH010000136         5718 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000136 DAZKBH010000000
VERSION     DAZKBH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.138"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..2134
                     /locus_tag="ACG8OO_002565"
     CDS             2..2134
                     /locus_tag="ACG8OO_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFP9046404.1"
                     /translation="MQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTDLEGTSAF
                     GLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKTKGNVLWI
                     ADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWKQKGLVFH
                     IVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVLFEYSRSY
                     LLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDALASATLSI
                     FLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEMAAGAAVN
                     SSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIKHLIQLIS
                     SALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQVANNRLNE
                     IFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHSKTAIV
                     GVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQESFFFSGTI
                     IENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQRQRLAL
                     ARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKACDQILV
                     LHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2103..3518
                     /locus_tag="ACG8OO_002566"
     CDS             2103..3518
                     /locus_tag="ACG8OO_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9046405.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3551..4585
                     /gene="sppA"
                     /locus_tag="ACG8OO_002567"
     CDS             3551..4585
                     /gene="sppA"
                     /locus_tag="ACG8OO_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9046406.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4587..5108
                     /locus_tag="ACG8OO_002568"
     CDS             4587..5108
                     /locus_tag="ACG8OO_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFP9046407.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5105..5491
                     /locus_tag="ACG8OO_002569"
     CDS             5105..5491
                     /locus_tag="ACG8OO_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFP9046408.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(5536..>5718)
                     /locus_tag="ACG8OO_002570"
     CDS             complement(5536..>5718)
                     /locus_tag="ACG8OO_002570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046409.1"
                     /translation="LKSEEYLIEKLNLLAAFPFSINTTIRECLFFEKIRINKRHVVFL
                     STCLPSTTSYHFSKRI"
BASE COUNT         2051 a          777 c         1080 g         1810 t
ORIGIN      
        1 tgtgcagcaa caagatgaaa aagattgtgg tgttgcttgc ctttcgatga ttttaaaata
       61 ttttaaaact gatatcccga ttcataaatt acgggaccta tctgggactg atttagaggg
      121 tacttcagca tttggattga aaaagacatt tgaaaaattg tcttttgatt gtatggctat
      181 tcaagcagat agtgagatct ggaaagaaag agatcttatc ttaccattaa ttgcacatat
      241 attggttgat aacagctata tgcactacgt tgtaatttat aaaacgaagg gaaacgtact
      301 atggattgca gatcccgcta atggaaaaaa tagaaaaacg attgaagcgt ttcaaaaaga
      361 atggacaggt attcttttat tacctatgcc tagagaaact tatattccta ttaaagaaaa
      421 agtagctgga ttaagttctt ttttgcctat aatttggaaa caaaaaggat tagtattcca
      481 catcgtttta gcatcgattt tcattacgtt ttttggcata ggaagctcat attattttca
      541 aggtattctt gattttttca tccctaatca agcacgttca actttaaata taatttccat
      601 tggcttgatc gctgtctatt tatttcgggt attgtttgaa tatagtcgta gttatcttct
      661 tattatttta ggacagcgaa tgagtatggc aattatgctt cagtatttca aacatgtgtt
      721 aactttacca atgaattttt ttgctacgag aaaatcaggg gaaattattt ctcgttttct
      781 tgatgccagt aagattattg atgctttagc aagtgctacg ttatcaatat ttttagatat
      841 tggtatggtg ttgcttgttg gggttacgtt agctattcag aatagtacgt tgtttttaat
      901 cactctatgc tctttgcctt tttatgtagt tgcaatattc gctttcgtga aaagctatga
      961 aaaatcgaac agggaagaaa tggcagccgg tgcagctgta aattctagta tcatagaaag
     1021 tcttaaagga atagaaacaa ttaaagcata tagcggtgaa gagaaagtat ataaccgtat
     1081 agaccatgaa tttgttaaat taatgaaaaa atcatttcgt acggttacgc ttgataatat
     1141 tcaacaagga attaaacatt tgattcaatt aattagcagt gcattaattt tatggctagg
     1201 ttcttatttt gttatggata atagaattag tctaggtcaa cttatcacat acaatgcttt
     1261 actagtattt ttgacagatc ctttacaaaa cattattaat ttacaagtga aaatgcaaac
     1321 ggctcaagtt gcaaataatc gtttaaatga aattttcgcg attgattcag aaaaatcgaa
     1381 ccagtctttt gaatatatgc tttctgaaca attatttcaa caagatatca ctgtaaaaaa
     1441 cgtttcattc tcgtatagca tgaaggcccc aacgttgaac gaaatttcat gtacaattcc
     1501 ttcgcacagt aaaacggcta tagttggagt tagtggttct ggaaaatcaa ctcttgcaaa
     1561 gttatttgtt cgtttttatg aagcatcaga aggatcgatc cattatggaa acattaatca
     1621 cttagacatt ccttttcaga tattacgcga tcgagtgaca tatttgcctc aagagtcatt
     1681 tttctttagt ggaacaatca ttgagaatct tatattcggt ttagatagcc caccaagttt
     1741 cgaacaaata atagaagttt gtgagatagt ccaactaaat gaatttatta atcaacagcc
     1801 attacgttac gagacattgt tggaagaagg tggaaataat ctttcaggag ggcaacgaca
     1861 acgattagcc ttagcacggg ctttattaaa aaatccggac atattaattt tagatgaagc
     1921 aactagtgga ttagatacct tgttagaaca tgcaattatg aattatctcc tcaatttaaa
     1981 agaaaaaacg attattttta ttgcgcatca tttaccaata gcaaaagcat gtgatcaaat
     2041 tttagttctc catgaagggc atttggtaga aaaaggaacg cacgatgaac ttcgttttgc
     2101 cgatggtatc tatcaaaaat tatgggaaat ctaggagagg gaaacaattt atgcagaata
     2161 ataattggtt cgaacattct agtatctata ctaaacaaca tcatagcttc taccgttggg
     2221 tgttataccc tgtaattctt tttttatgta ttattggatt gtttttaacc tttgctaaaa
     2281 aagaggttgt tattcggaca acggctcaat tgaaccccga aaaaatagag aagttacaag
     2341 tgccattaga agctaaaata atggaaaact tcctaaaaga aaatcaattt gttcataagg
     2401 acgatgtgtt ggttagattg gattgttctt taatagagaa tgaaaaggct cagatagaac
     2461 aagaaaacca acgtattact caacaaataa agatggctca gctatttatt gaaagtataa
     2521 gtaaaggaaa aaatttgttt tcaacggatg acagttttgg ctacagtaat caattaaaga
     2581 gcatgttgtc agaaaaagaa tcactccgct acgctttgaa gcaaagtgaa ttaaatgatc
     2641 aaaagcaatt agaagtatac gaaaagacaa aaagacaact agaaaaacaa attgagagtt
     2701 cagatagtaa attacaagaa tggcaacaag tacaggtagc ttggagtaat aatcaatcat
     2761 taaaagattt ttcaaaagaa atgatggcaa actatgagaa ttggcaagaa caactaaata
     2821 atgtttctga tgatcaaaaa aatcaagtga aactgacaat ttcagcaagc ataaatgaac
     2881 aaattgagca actaaaaaaa gaagtagaac agtatcagtc agaaaaagct aaattagtta
     2941 aaccaactac ttctgagaat gacagaatta gtcaaacgga aaaaggaaag caagagctag
     3001 aacaagcaat aacaactgta aaacaaacag ttacagagct aaatgaaaaa caagagaaga
     3061 accagtcagt tatcaaaagt ttggacgatc aactttcaaa aggaatacta aaagctcctg
     3121 tatctggaac ggttcattta aatgaagaaa caaagggaca agcagaacta gccaaaggaa
     3181 cagtgcttgc agaaatttat ccgatccacg aaaaatcaaa aatgaaattt acagcacttt
     3241 tacctgcgaa tgaaagtacc tatattaaag aagggatgaa ggttcatttt aaattggatc
     3301 aaaaaggtgg ggctccgatt acgatcgacg gaactttaga tgaaatttca gaaaattcta
     3361 ctgccactga gaaaggtgtt ttttatgcag tgaaaggaac gttaaaaaca actcaatcgt
     3421 ttccgtttcg ttatggatta actggagagt tgtctttaat tgtaggagaa aaaacatatt
     3481 ggcaacaaat tgtcgatgtg tttctaaata aaaaataatt atttttacta agaatatagg
     3541 aggagtttat atgaataaaa gaagatggat tgctgtggga gtagcgtgcg gattattgtt
     3601 actatccatt atcgtcgcgc taattccagg aaaagataaa gaggaagcat caaatacaac
     3661 cctaacagga ataaataaga ttttttacgg atctaacgaa atcacagaag aaacgttaga
     3721 agaaggcgca tcaaataaaa agattgtcaa actatccgtc aatggagtta ttgcagatac
     3781 aggtgaaagc aatcttttta gtagggaaca atataaccat cagaattttt taacacagct
     3841 aaagaaaatt caagaagaca aagcggttaa gggagtgtta ttagaagtaa attcacctgg
     3901 gggaggaata tatgaaagtg ctgagattgc taaagaaatg gcaaaaatta aaaagctaga
     3961 tattcctatc tatactgcat ttaaaaatac agcggcaagt ggagggtact acatttctgc
     4021 tggttcagat aaaatatttg ctacggaaga aacaacaact ggatcaattg gtgtcattat
     4081 ttctggttta aattattctg gcttattaga aaaacttggc gtaactgatg cgacgtataa
     4141 aagtggtgca ttaaaagata tgatgtcacc tcaacacaaa ccttcagagg aagagaataa
     4201 agttatacag gaatttgtga tgagtgctta tgatcgtttt gtcaatgttg tagcaaaagg
     4261 aagaaatatg gatactaatg cagtgaaaga acttgcggat ggaagaattt atgatggtaa
     4321 ccaagcagta gaaaatgggt tggtggatca aattggttac tctgaagatg cattagattc
     4381 cctaaaaaaa gagaagaaat taactgatgc gacggttata gaatataaaa atgacacaac
     4441 tggctttgct tcttcttggt taggtaataa gattgctgaa tggcaagggc taaaggcaac
     4501 tacatcagat cgtgtaattt cagtttttga aaaacttgga ttttctgata ctccaaaacc
     4561 aatgtattac tatggagggc tataaaatga ataagaagag tggcattgaa actttgccta
     4621 attatgtatt tgcgggattt tggttacgat tttttgcttt tcttattgat ttaatttgta
     4681 ttagcatcat cacccatgct actataggtc tcttttacaa aatcatggga ctggaatctg
     4741 acaaatccat tattactttt tatggtatag cgtcattagc tatttattta acctattttg
     4801 tacttttgac taagctgaat tcaggacaaa caattgggaa aatgattttt gggataagag
     4861 tagtgagctt tgaggaaaaa gagctaagtt ggcaaactat ctttattcgt gagtgtatat
     4921 gtaggtttat attaaaaaat tttgtatttc aaattgggta tttggtggct gctttcacgc
     4981 ccaagaaaca acatattggg gattattttt ctaacacgtc agtggttgtt attcgttcat
     5041 tagaagctat taactatggt caaaaaattt ctaattctaa aacacttaat ctggagagaa
     5101 ttaaatgaat aagagaaaaa cagagtttat tttgatttta attagtggaa taacagggat
     5161 tttatgtagt cttttagcat tgatgggtgg tatgtttcta tcttctattg attcaactac
     5221 tttaaatact acagaactaa ccagtaagga tatagatatg ttagtcaatc taagcgggaa
     5281 tgttgtttta tttggaggaa ttaccctagt tttttcgata atgattttca taataggttt
     5341 tttagtaaga aaaaatcaaa aaacagtggt ctatggtatt agtttactca ttttatctat
     5401 tattccattt tttcttttaa cattattgtg ggtaattcct ggagttttag gtattattgc
     5461 gggattgatg cttctaataa gaaaatctta attaggcaat gtgtagacaa cgtgaaacga
     5521 ttaatcaatt aaaagctaaa tacgttttga gaaatgataa cttgtagtcg atggaagaca
     5581 tgtagataaa aaaactacgt gtcttttatt tattctaatt ttttcgaaaa aaagacactc
     5641 tctgattgtt gtattaatgg aaaaggggaa agcagcaagt aaatttagtt tttctattaa
     5701 atattcttca ctttttaa
//
LOCUS       DAZKBH010000137         5614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000137 DAZKBH010000000
VERSION     DAZKBH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.139"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..2008)
                     /locus_tag="ACG8OO_002571"
     CDS             complement(59..2008)
                     /locus_tag="ACG8OO_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9046410.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="ACG8OO_002572"
     CDS             complement(2050..3108)
                     /locus_tag="ACG8OO_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFP9046411.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="ACG8OO_002573"
     CDS             complement(3227..3682)
                     /locus_tag="ACG8OO_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046412.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="ACG8OO_002574"
     CDS             complement(4266..5069)
                     /locus_tag="ACG8OO_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9046413.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2076 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat attt
//
LOCUS       DAZKBH010000138         5565 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000138 DAZKBH010000000
VERSION     DAZKBH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.140"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACG8OO_002575"
     CDS             complement(<1..281)
                     /locus_tag="ACG8OO_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9046414.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            453..1541
                     /locus_tag="ACG8OO_002576"
     CDS             453..1541
                     /locus_tag="ACG8OO_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9046415.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="ACG8OO_002577"
     CDS             1669..3045
                     /locus_tag="ACG8OO_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9046416.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="ACG8OO_002578"
     CDS             3446..5026
                     /locus_tag="ACG8OO_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9046417.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="ACG8OO_002579"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="ACG8OO_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
BASE COUNT         1870 a          913 c         1136 g         1646 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctt
//
LOCUS       DAZKBH010000139         5341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000139 DAZKBH010000000
VERSION     DAZKBH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.141"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..285)
                     /locus_tag="ACG8OO_002580"
                     /pseudo
     CDS             complement(61..285)
                     /locus_tag="ACG8OO_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(839..925)
                     /locus_tag="ACG8OO_002581"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ACG8OO_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2091..3131
                     /locus_tag="ACG8OO_002582"
     CDS             2091..3131
                     /locus_tag="ACG8OO_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9046418.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3955..4287
                     /gene="traE"
                     /locus_tag="ACG8OO_002583"
     CDS             3955..4287
                     /gene="traE"
                     /locus_tag="ACG8OO_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFP9046419.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4906..5247
                     /locus_tag="ACG8OO_002584"
     CDS             4906..5247
                     /locus_tag="ACG8OO_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP9046420.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1770 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtggtat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cacaatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     4141 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     4201 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     4261 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     4321 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     4381 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     4441 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     4501 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     4561 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     4621 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     4681 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     4741 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     4801 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat
     4861 ataaaacata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     4921 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     4981 ggatttgtga cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattagac
     5041 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     5101 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     5161 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     5221 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     5281 tagaaggagt cacgatgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
     5341 t
//
LOCUS       DAZKBH010000140         5289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000140 DAZKBH010000000
VERSION     DAZKBH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.142"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..339)
                     /locus_tag="ACG8OO_002585"
     CDS             complement(19..339)
                     /locus_tag="ACG8OO_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP9046421.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(487..2721)
                     /locus_tag="ACG8OO_002586"
     CDS             complement(487..2721)
                     /locus_tag="ACG8OO_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9046422.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3200..3466
                     /locus_tag="ACG8OO_002587"
     CDS             3200..3466
                     /locus_tag="ACG8OO_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFP9046423.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3466..5193
                     /gene="ptsP"
                     /locus_tag="ACG8OO_002588"
     CDS             3466..5193
                     /gene="ptsP"
                     /locus_tag="ACG8OO_002588"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9046424.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         1595 a          984 c         1066 g         1644 t
ORIGIN      
        1 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
       61 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
      121 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
      181 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
      241 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
      301 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
      361 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
      421 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
      481 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
      541 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
      601 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
      661 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
      721 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
      781 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
      841 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
      901 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
      961 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     1021 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     1081 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     1141 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     1201 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     1261 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     1321 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     1381 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     1441 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     1501 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     1561 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     1621 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     1681 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     1741 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     1801 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     1861 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     1921 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     1981 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     2041 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     2101 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     2161 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     2221 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     2281 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     2341 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     2401 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     2461 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     2521 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     2581 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     2641 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     2701 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     2761 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     2821 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     2881 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     2941 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     3001 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     3061 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     3121 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     3181 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     3241 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     3301 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     3361 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     3421 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     3481 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     3541 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     3601 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     3661 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     3721 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     3781 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     3841 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     3901 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     3961 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     4021 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     4081 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     4141 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     4201 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     4261 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     4321 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
     4381 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
     4441 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
     4501 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
     4561 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
     4621 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
     4681 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
     4741 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
     4801 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
     4861 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
     4921 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
     4981 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
     5041 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
     5101 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
     5161 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
     5221 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
     5281 tagacaaaa
//
LOCUS       DAZKBH010000141         5029 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000141 DAZKBH010000000
VERSION     DAZKBH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.143"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(406..780)
                     /locus_tag="ACG8OO_002589"
     CDS             complement(406..780)
                     /locus_tag="ACG8OO_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9046425.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="ACG8OO_002590"
     CDS             complement(803..2203)
                     /locus_tag="ACG8OO_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFP9046426.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="ACG8OO_002591"
     CDS             complement(2207..2617)
                     /locus_tag="ACG8OO_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9046427.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="ACG8OO_002592"
     CDS             complement(2635..3126)
                     /locus_tag="ACG8OO_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9046428.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="ACG8OO_002593"
     CDS             complement(3136..3927)
                     /locus_tag="ACG8OO_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9046429.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="ACG8OO_002594"
     CDS             complement(3920..4693)
                     /locus_tag="ACG8OO_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9046430.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>5029)
                     /locus_tag="ACG8OO_002595"
     CDS             complement(4849..>5029)
                     /locus_tag="ACG8OO_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFP9046431.1"
                     /translation="RIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPK
                     GEYELIYTIFEQTIF"
BASE COUNT         1600 a         1023 c          661 g         1745 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgt
//
LOCUS       DAZKBH010000142         4968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000142 DAZKBH010000000
VERSION     DAZKBH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.144"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..1122)
                     /locus_tag="ACG8OO_002596"
     CDS             complement(7..1122)
                     /locus_tag="ACG8OO_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046432.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="ACG8OO_002597"
     CDS             complement(1167..1523)
                     /locus_tag="ACG8OO_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046433.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="ACG8OO_002598"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="ACG8OO_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFP9046434.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="ACG8OO_002599"
     CDS             complement(2991..3830)
                     /locus_tag="ACG8OO_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP9046435.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="ACG8OO_002600"
     CDS             4015..4902
                     /locus_tag="ACG8OO_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9046436.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         1619 a          979 c          736 g         1634 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatt
//
LOCUS       DAZKBH010000143         4894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000143 DAZKBH010000000
VERSION     DAZKBH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.145"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..466)
                     /locus_tag="ACG8OO_002601"
     CDS             complement(26..466)
                     /locus_tag="ACG8OO_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9046437.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            626..1132
                     /locus_tag="ACG8OO_002602"
     CDS             626..1132
                     /locus_tag="ACG8OO_002602"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP9046438.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1300..3213
                     /locus_tag="ACG8OO_002603"
     CDS             1300..3213
                     /locus_tag="ACG8OO_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9046439.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3218..4219
                     /locus_tag="ACG8OO_002604"
     CDS             3218..4219
                     /locus_tag="ACG8OO_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9046440.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4421..4627)
                     /locus_tag="ACG8OO_002605"
     CDS             complement(4421..4627)
                     /locus_tag="ACG8OO_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFP9046441.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         1804 a          751 c          995 g         1344 t
ORIGIN      
        1 gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat
       61 cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa
      121 ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga
      181 taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac
      241 tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta gagagcattt
      301 cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt
      361 gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc
      421 gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata
      481 gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt
      541 aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc
      601 aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga
      661 tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc
      721 attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca
      781 acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc
      841 tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa
      901 tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc
      961 agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat
     1021 gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc
     1081 tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc
     1141 ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg
     1201 agaaaataat cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt
     1261 ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa
     1321 caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg
     1381 acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa
     1441 actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa
     1501 acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac
     1561 caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa
     1621 gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat
     1681 ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca
     1741 gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat
     1801 ggtgtagata aagagattat caaacatctg attgctactt atctattgta cgatatcgct
     1861 tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct
     1921 acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa
     1981 caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca
     2041 gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac
     2101 aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat
     2161 agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata
     2221 aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga
     2281 gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt
     2341 gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta
     2401 gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat
     2461 gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat
     2521 acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat
     2581 ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga
     2641 gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac
     2701 catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata
     2761 aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag
     2821 ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa
     2881 ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt
     2941 ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca
     3001 gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt
     3061 cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact
     3121 ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt
     3181 atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt
     3241 gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat
     3301 cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca
     3361 agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga
     3421 aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga
     3481 aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa
     3541 aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa
     3601 tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga
     3661 agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat
     3721 tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga
     3781 agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt
     3841 tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc
     3901 tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc
     3961 agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt
     4021 cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt
     4081 tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa
     4141 ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt
     4201 aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct
     4261 ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact
     4321 tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac
     4381 acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc
     4441 gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg
     4501 gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac
     4561 aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag
     4621 ctttcataag cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa
     4681 atgtgacaag tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat
     4741 ggctaatgat aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac
     4801 gtctagacca ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa
     4861 agaatttccc caaaaattcg atgtatccta aact
//
LOCUS       DAZKBH010000144         4839 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000144 DAZKBH010000000
VERSION     DAZKBH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.146"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACG8OO_002606"
     CDS             complement(220..972)
                     /locus_tag="ACG8OO_002606"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9046442.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="ACG8OO_002607"
     CDS             1085..2005
                     /locus_tag="ACG8OO_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9046443.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACG8OO_002608"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACG8OO_002608"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9046444.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="ACG8OO_002609"
     CDS             3311..4789
                     /locus_tag="ACG8OO_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9046445.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1697 a          778 c          935 g         1429 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agaggctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgtt
//
LOCUS       DAZKBH010000145         4836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000145 DAZKBH010000000
VERSION     DAZKBH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.147"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1672)
                     /locus_tag="ACG8OO_002610"
     CDS             complement(62..1672)
                     /locus_tag="ACG8OO_002610"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFP9046446.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1894..2139)
                     /locus_tag="ACG8OO_002611"
     CDS             complement(1894..2139)
                     /locus_tag="ACG8OO_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046447.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2865..4205)
                     /gene="glnA"
                     /locus_tag="ACG8OO_002612"
     CDS             complement(2865..4205)
                     /gene="glnA"
                     /locus_tag="ACG8OO_002612"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFP9046448.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4233..4625)
                     /locus_tag="ACG8OO_002613"
     CDS             complement(4233..4625)
                     /locus_tag="ACG8OO_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9046449.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1416 a         1044 c          793 g         1583 t
ORIGIN      
        1 tgacaagaaa attgcttatc cccttctctt gctacttttc tctttctatc aagttcaaaa
       61 attattttgt ttcgtgatct gctaatgatg ctccgacaaa tgctttgatt aatttttgcg
      121 ggcgatttgg acgagaaatc aattctgggt ggaattggca tcccacaaag aattttttct
      181 ctggcagctc tacgatttct actaaacggt tatctggtga gacacctgaa aagactagcc
      241 cttgttcttc aaacaattga cgatatttgt tattgaactc atagcggtga cggtgacgtt
      301 cttgcacaac atcggctcca tcatatgctt cagccgtctt cgttcctttt ttgattttac
      361 atgggtataa tccaaggcgt aaagttccac ccaaattctc gatgttttcc tgatcagcca
      421 ttaaatcaat aatattgttt gctgtatctg gattcgtttc agctgatgac gcatcttcta
      481 atccaacgac gttacgtgca aattcgacac aagccatctg cataccaaga caaataccta
      541 aaaacggaac gtcattttcg cgagcataac gaatcgcttc gattttccct tctactccgc
      601 gatcaccaaa tccaccagga accaagattc catctgcgtc tttcaaacgt tcttctacat
      661 tttctctcgt taattcttgt gctttgatcc aatcgatctc gatgtctgaa ttataggcga
      721 aaccagaatg tttcaatgct tcaactactg agatatatgc atctggtaac tcgacatatt
      781 taccgacaag cgcaatccgt acttttttct tcaagttcaa gacacgttct tctaattctt
      841 tccattctgt catgtctgct tctggtgtat ctagttttaa atggtcgcaa acaatttgat
      901 ccattccttg tgcttgtaaa actaatggga tggagtaaag tgtatctaca tctggcgact
      961 cgataactgc ttctggttcg acatcacaaa attgtgccaa tttgtttttc acgctttgag
     1021 aaacaggttg ctctgtacgt accaccaaaa tattcggttg gatacctaaa ccacgtagct
     1081 cttttacact gtgctgcgtt ggtttagttt tcatttctcc agcagctttc aagtaaggaa
     1141 tcaatgttgt atggatatac ataacattat ctgtaccgac atctgctttc atttgacgaa
     1201 gtgcttcaag gaatggcaaa gattctatat cccctactgt tccccctact tctgtaatga
     1261 tcacatctga atcagtcatt ttagctgctc gcatgatttt ttctttgatc tcatttgtaa
     1321 tatgaggaat aacttgaaca gttgctccca agtattcgcc cttacgttct ttacgcaaaa
     1381 cttcagaata gatttttcct gtcgtcacgt tggagtattt gttcaaattg atgtcaataa
     1441 aacgttcgta gtggcccaga tccaaatctg tttcagcacc atcatctgtt acgaatactt
     1501 ctccgtgctg atatgggctc atcgtccccg gatccacgtt gatgtatgga tcaaattttt
     1561 ggatagttac ttttaagccg cggtttttca ataagcgtcc taaagaagct gcaacaatcc
     1621 cttttccgat tgatgaaacg acaccgccgg tcacaaaaat atattttgtc ataagttcaa
     1681 aaactccttt ttaatttatg tgcaggaaaa gtctcagaca ataaacaaag ctccctattc
     1741 ttcaataaga atagggagcc gaaatttctg taaacttctg accctctaaa agggtgccca
     1801 aggtgtataa tacaagctga ctaagggaaa gtcaagtact caaatgaata ctatccgctt
     1861 acaatttttt ctttttcgat atcctttgag aaattatttc ttaagtcctt tatgagtgca
     1921 aaaaaaattt aacccacata ataagcttga tataaacata gctaacgtta aacctaagca
     1981 aatcagaaaa tcgttactga aaaattttaa gtgaaataat agataaaaaa ataaaacaaa
     2041 cattatgata gataatatcc cacctaacaa agaactactt tttcctactt gcagcaacag
     2101 tttatatagc ttatcgtaga aacaatcttt ttctttcacc cttatttacc tcctttacgt
     2161 aaaaaacgta gtccacagtt ctcggatttt tggcatatct tttctattaa ataatatcaa
     2221 aaacaaaaca attccactaa ttttttcaaa gatgaccttt ttttcaacaa aagtgccttg
     2281 tagggattca ttgaaggtaa cgaatcacat aagtaaaaaa taaggagatg cgacaaaagt
     2341 cgtgcctcct tatttctaaa taaacggtgt tctcctgcca actcctcggt cacaagtcac
     2401 aatatccatc aaacatgaga agctgttttt gaaattattt cttgtgattc ggagttaaat
     2461 ggtaggctca caacctctgg ataaacggta ttcaaaagcc agtttctcgg tattaagcca
     2521 aatttatgca aaaatgtaga aagccatttt cacaaaattt ttcttaatac tcgaaactaa
     2581 acgtcttttt caaaacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag
     2641 ttcagccaat tcaaaaacaa gaaaagctgt ttttgcttgg ctattcttat tacttgttgc
     2701 agggcagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
     2761 agctcaaaaa acggcaaaat atgaggaact atttttccgt tagtttctct aatactcagg
     2821 tcaaaacgct gatttcacag cctctaattc acggtattcc tgttttaata aagttctaga
     2881 tattgttctc tttcccactc ggaaacagtt tgtctgaatg ccgcccattc gagacgtttt
     2941 gcttcaacaa aatttgagaa gatatgttgt cctaatgcgt caatcattac attatcctta
     3001 cgcaattctt tgatagcatt atggatagtt gaaggtaagt cctggatatg tgcagcttct
     3061 ctttcttcct catccattac ataaatgtta cggtcaacag ctgctggcgg tgtgatctga
     3121 ttacggatac cgtctagtcc tgcttgcaac aatactgcca ttgccaagta tgggtttgca
     3181 gatggatcaa ctgaccgcaa ttccaaacgt gtggataaac cgcgagattc tggtacacgt
     3241 actaatggcg aacgattacg gccactccat gcgacataaa caggcgcttc ataacctgga
     3301 acgagacgtt tataagaatt gactgttgga ttgcaaacgg ctgtgtaggc acgagcatgt
     3361 ttcagcaaac cacctaagaa atgataagct gtttcactta gttccaattc accgtcttta
     3421 tcatagaaaa catttccttc atcattgaac aaagacatat tgcaatgcat accagaacca
     3481 ttgatcccaa ataacggttt cggcatgaaa gtcgcgtgaa gtccatgctt gcgtgcaatc
     3541 gttttgacaa cgagcttgaa ggtttggata ttatcacaag catctaccac atctgcatat
     3601 ttaaagtcaa tttcatgctg tcctggcgct acttcatgat gtgaagcttc cacttcaaag
     3661 cctagacttt ctaattccaa cacgatgtca cggcggcaat tttcacctaa atctgttggt
     3721 gcaaaatcaa aataaccacc tttatcattc aaggtcgttg tgatcccacc gtcttcgtct
     3781 aatttaaata agaagaattc tggttcaggt cctaaattga aagaggtaaa accaaggtcg
     3841 cgcatatctt tcaatgctcg tttcaaattc ccgcgaggat cgccggcaaa tggtgtccca
     3901 tccggattat agatatcaca aattaaacga gcaacttttc catgagcact ttcccacggg
     3961 aagatcatcc aagtggagag atctggatac aaatacatat cgctttcttc aattcgaaca
     4021 aatccttcaa tcgaagaacc gtcaaacatc attttatttt ctaatacttt gtcgatctgg
     4081 ctcactggaa cttctacatt tttgatcgta cctaagatgt ctgtgaacat caatcgtaaa
     4141 aatcgtacgt tttcttcctc aatgattcgt ttgatttctt cacctgttat ctgtttcttt
     4201 accatggtat ccctcttcct tttttaattt attcagtcaa agaattgtct ttgcaatcct
     4261 tgcgactgat aatgacctgt tttgcttaac ccaccttgcg ccaaaatctc atcacgaaaa
     4321 atttgtcgaa cgtcatgatc tgtcaacggc ttcttgtttt ttgcttcttg ttttttcgcc
     4381 tgtgccattt cgtatacacg tttgatccca gccatattca atccctctga aagatagtct
     4441 ttaatctcaa gcaacatatc aatatcattc agcgaataca tacgtcggtt tccttcgctt
     4501 cgttccggat gaactaaatc ttgttcttca taatagcgaa tttgccgagc agacaaatca
     4561 gtcagcttca taactgtacc aattggaaat acggacatag atcggcgcaa ttccttttct
     4621 cccatcagac gtcccctttc gttttataag ttggctaaag tgtaccaatt aaaaaaaata
     4681 ttgtcaactg catatgttag gttttctaac atgagatctc tattttttat aaaataacaa
     4741 aaaaataaga acacaaaaaa ggtatctagt tgattttcgt accagatacc ttttattttt
     4801 tattatactg tactatcctt tattagtgtt tttatt
//
LOCUS       DAZKBH010000146         4714 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000146 DAZKBH010000000
VERSION     DAZKBH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.148"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..359
                     /locus_tag="ACG8OO_002614"
                     /pseudo
     CDS             108..359
                     /locus_tag="ACG8OO_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            452..1246
                     /locus_tag="ACG8OO_002615"
                     /note="aph(3')-IIIa"
     CDS             452..1246
                     /locus_tag="ACG8OO_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFP9046450.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1656..1739
                     /locus_tag="ACG8OO_002616"
                     /pseudo
     CDS             1656..1739
                     /locus_tag="ACG8OO_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1788..1871
                     /locus_tag="ACG8OO_002617"
     CDS             1788..1871
                     /locus_tag="ACG8OO_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFP9046451.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1996..2733
                     /gene="erm(B)"
                     /locus_tag="ACG8OO_002618"
     CDS             1996..2733
                     /gene="erm(B)"
                     /locus_tag="ACG8OO_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP9046452.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2996..3859)
                     /locus_tag="ACG8OO_002619"
     CDS             complement(2996..3859)
                     /locus_tag="ACG8OO_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP9046453.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3861..4133)
                     /locus_tag="ACG8OO_002620"
     CDS             complement(3861..4133)
                     /locus_tag="ACG8OO_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9046454.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4150..4365)
                     /locus_tag="ACG8OO_002621"
     CDS             complement(4150..4365)
                     /locus_tag="ACG8OO_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP9046455.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4457..>4714)
                     /locus_tag="ACG8OO_002622"
     CDS             complement(4457..>4714)
                     /locus_tag="ACG8OO_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFP9046456.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1447 a          796 c          865 g         1606 t
ORIGIN      
        1 atttcttggg agttttgaga ctactatatg cgtttttttg ttgatttacc aacatgttta
       61 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
      121 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      181 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      241 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      301 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      361 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      421 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      481 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      541 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      601 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      661 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      721 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      781 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      841 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      901 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      961 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     1021 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1081 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1141 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1201 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1261 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1321 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1381 agcgcctacg aggaatttgt atcgataaga aatagattta aaaatttcgc tgttattttg
     1441 tacatttaac ttgacggtga catctctcta ttgtgagtta ttagtggtac agttttcaac
     1501 cgttttaatt ataaaaaagt ggtgcatttt taaattggca caaacaggta acggttattg
     1561 caggtgtatt tcttatctat gggtttaaca tggattttat cattaaaatc atgagtattg
     1621 tccgagagtg attggtcttg cgtatggtta accctaaagt tatggaaata agacttagaa
     1681 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
     1741 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
     1801 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
     1861 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
     1921 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
     1981 ttcaggagtg ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
     2041 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
     2101 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
     2161 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
     2221 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
     2281 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
     2341 gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
     2401 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     2461 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     2521 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     2581 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     2641 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     2701 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     2761 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     2821 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     2881 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     2941 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     3001 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag ctttccaatt
     3061 tttgttgaat tgctttaaat tcaggtgtct cttggtgttt atttagaacc attttttgct
     3121 ctattcgttc taaagtcggt tgaatttctt tccctgatac tttacgattc aattcttttt
     3181 ctaaggtttc ttttggacta atagaaggtg tttccaaact tgaatagagt tttactcctt
     3241 ctctgttata tagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     3301 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgccctg gctgtcattg
     3361 gatcatctgc atacatggtt tcatatcgtt caattgttcc taaatatgag ttgattttag
     3421 gtactgccat gacatacatt tttgtttcat aacctttggc ttgaagcatt gttgcggttt
     3481 gaataggaac gtctgttgtt cgtcctgtac cttcgatcac caaattatac ccttgatcgc
     3541 tcaaacggct tatgatcgct tctgtcatgc gattagaata aggggtaacg tgttttacta
     3601 cgtctttttc ataaagtttc actagttcat caaaattagg gtgctgttgt ttaaaggtat
     3661 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     3721 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgcttttt
     3781 ttccttgaat caattcttct aaattatcat ttaagcgatt ctcaaattgt ttgtcagtaa
     3841 aattgactat atttgccata ttaagccact ttctctttat tcaaaacttg ttttgagtaa
     3901 cgattcattg accgccaata ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa
     3961 agatttacac gttttgcaag ctttaattgg tttaataaat tgacttccaa taattgagag
     4021 tcatttttat ttaattcatg gttcaaaaca tagtttaata ctcgattata aacgcttgcc
     4081 gataattcgt taatgatctc tatttcaaat gttttttcat acgtaactgc catttcattt
     4141 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     4201 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     4261 gtcagcacga acacgaaccg tcttatctcc cattatatct ttttttgcac tgattggtgt
     4321 atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact tctttctatt
     4381 tcttcttact cttattttat catcaacaat cacaaatcac aagtgatttg tgattaatca
     4441 cttgtgattt gtgattctat tctttttcgt tttctaattg aataattcgc tcaagcattt
     4501 caaaaaatac gttcttatac atggataaaa cttttttgtc atagccttta tgctctgtaa
     4561 tgccgttttt agaccaggta cttactttat tacttcgctt gataatattt tggaaaacca
     4621 agttatcttc tttatgttgc ttatacagtt cttccaggtt tgattttatc gttgcgctgt
     4681 ccgtatcaac taaataagga acaaaaccga tcat
//
LOCUS       DAZKBH010000147         4697 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000147 DAZKBH010000000
VERSION     DAZKBH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.149"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACG8OO_002623"
     CDS             <1..649
                     /locus_tag="ACG8OO_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046457.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="ACG8OO_002624"
     CDS             708..1148
                     /locus_tag="ACG8OO_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046458.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="ACG8OO_002625"
     CDS             1160..1828
                     /locus_tag="ACG8OO_002625"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9046459.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="ACG8OO_002626"
     CDS             1892..2515
                     /locus_tag="ACG8OO_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFP9046460.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="ACG8OO_002627"
     CDS             2571..2957
                     /locus_tag="ACG8OO_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFP9046461.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="ACG8OO_002628"
     CDS             3170..4561
                     /locus_tag="ACG8OO_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFP9046462.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1559 a          782 c         1000 g         1356 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttg
//
LOCUS       DAZKBH010000148         4645 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000148 DAZKBH010000000
VERSION     DAZKBH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.150"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..717)
                     /locus_tag="ACG8OO_002629"
     CDS             complement(133..717)
                     /locus_tag="ACG8OO_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9046463.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="ACG8OO_002630"
     CDS             complement(928..1131)
                     /locus_tag="ACG8OO_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046464.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="ACG8OO_002631"
     CDS             complement(1141..1563)
                     /locus_tag="ACG8OO_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046465.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="ACG8OO_002632"
     CDS             1801..2415
                     /locus_tag="ACG8OO_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9046466.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="ACG8OO_002633"
     CDS             complement(2580..3404)
                     /locus_tag="ACG8OO_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046467.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="ACG8OO_002634"
     CDS             complement(3428..3583)
                     /locus_tag="ACG8OO_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046468.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="ACG8OO_002635"
     CDS             complement(4042..4299)
                     /locus_tag="ACG8OO_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9046469.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="ACG8OO_002636"
     CDS             complement(4292..4561)
                     /locus_tag="ACG8OO_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9046470.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1670 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tctta
//
LOCUS       DAZKBH010000149         4639 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000149 DAZKBH010000000
VERSION     DAZKBH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.151"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..880)
                     /locus_tag="ACG8OO_002637"
     CDS             complement(<1..880)
                     /locus_tag="ACG8OO_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9046471.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVD"
     gene            complement(882..2321)
                     /locus_tag="ACG8OO_002638"
     CDS             complement(882..2321)
                     /locus_tag="ACG8OO_002638"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP9046472.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2506..4452
                     /locus_tag="ACG8OO_002639"
     CDS             2506..4452
                     /locus_tag="ACG8OO_002639"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFP9046473.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1488 a          785 c          761 g         1605 t
ORIGIN      
        1 tatcaacaat aagacttgca atttcatcaa ttggttgtct tattgttgtc aattgtggga
       61 aaaatttctc aataaaagag gtaccatcgt atccaataat ttttaaatct tctgggatat
      121 taagtttaag ttgatgagca attttcatag tgagaatggc tgttaaatca tcagaaacaa
      181 aaataccatc tggtttgtga tttataatag ttgactttat ttccatttca cgacggatag
      241 tagacagatt attaggaatt ttgaaaattt ttccatttgg attttgaaaa gaaaaaccta
      301 gtgcacgtaa ttctgtggga gaatccgtgt tatcatttcc tgtaatcata atggtatttt
      361 gacaaccatt tttttgtaaa gtcttagctg caagttttcc cccttcaaag ttatctgaag
      421 aaacaatgga aacattagga gctaaatttc tatcaaaagc aacaataggt gctctcacac
      481 gctcataatc agcaattcca agattatggc ttgatgagat tatgccatca acttggttgg
      541 cttcaagcat ttcaatgtat tctctctctt tagcaggatc attttgacta ttgcaaataa
      601 tcgttttata tccatgttta aataactcta tttcgagata ctcaatgagt tcagagtaaa
      661 agatattact aatattagga aaaataagac caataagctg aggtgacttt ccttgaagtc
      721 cacgtgctaa attatttggt ttataaccaa gagttttcat agcttcatga actttttgtt
      781 ttgttttttc tgataaatat cccttattat taatgacgcg tgaaacggtt gttgggctaa
      841 ctccagctaa ttcagcgaca tcagttaatt ttgctaccat tttaatattt taattcaaag
      901 tattttccag taggttcgcc tgatttgatt tgaataccat tttggtgatt ttcagggaat
      961 acacgactag taaacacttt ttctccttta ttaataaaaa tctcaatgat tgagttatct
     1021 acaaaaatgt tagcagttgt ttctttagta tcaattgagc attcacgaat cgtaccaaag
     1081 tcagtggcat attgaatgcc tgctttgctg cgatctaaaa taatttttcc ttctttggta
     1141 tctacaatca agctaagtcc atttccttta ctatcagaga aaagaagtaa ctccgatttc
     1201 tgatttggag gaaaagttag ttcaagctca taagtattat ttgtttctgc ttttgctgta
     1261 aattgttcag aactagcacg taatgatgta atagcttcta ctggatattg gtaaagttta
     1321 ccatctttta ttgaaagttc ttttacaaga ctcattgctc cctgataatc atacttatca
     1381 gttggataat cgatatcagg tagtccaatc caactgacaa tcagagcacg accatcagga
     1441 gtattgaatc cttgagtcgc atacgcttca aatccataat caagattttg aatttctgaa
     1501 gcgcccacaa gttttacttt ttctatgtca agcgattgac agatcttaaa agtattcgga
     1561 tagatattcc cataatttag ctcagattta tccaaccctt gaggacaata aatgagaaca
     1621 ggttttttgt ctacaaatac tagatttgga cactcaatca tgtattctga accagtgccg
     1681 ccaaaatcaa ggttgccaac ttcctcccat gtttccacgt tattgtcatc cgctttgtaa
     1741 agtttgataa agcctgattt ttctaaattt tgtgctccaa caatcgcata caattgtcct
     1801 tcataattaa acatttgtgg atcacgaaaa tgatcagtta catcagcagg tttatgaata
     1861 agaacattct cacctttcgt aatgttgtag tctttatcca tccatgctcc gatttgaagt
     1921 gtatcacgtt cccaattttc atttcgaacg tttcctgtat agagtaagaa tagattcccc
     1981 tctatttcat atgcagaacc ggaataagcg ccgtggctat ctcgttttgt gtctggaaga
     2041 agttctgctc ctgttacatg aaaatgaact aaatcatctg attcattgtg tacccattct
     2101 ttcaacccat gagcagctcc aaatggccaa ttttgataaa aaagcgtata ttttccgtta
     2161 aaaaaagaaa aaccattagg atcgtttaac aatcctgttt taggttcgat atgatagcta
     2221 gcacgccaag gtgatcgttt tacattatcc ttaattttat ccatctcatc tttagtccaa
     2281 tcagaataag cacgatatcg tatttctcga ggtaagttca tattttactc cttcaattta
     2341 tctttttaca actagtatgg taaacgtttt ccgttgaaaa atcaatcatt tcatttaaaa
     2401 aaataaacta tatgacaaac gtttgacata tttttaagat atgctaaaat aaatttgcat
     2461 ttcagtgaac cgttttcacg taaaaataaa aaaggagtga ttcgaatgaa ttacagtaaa
     2521 gtagcttccg acgtaataca agcggtcgga aaagataacc ttattgcagc tgcacactgc
     2581 gctacacgtc ttcgtctcgt actaaaagat gacacgaaag tgaatcaaaa agctttagac
     2641 gaaaatccag atgtgaaaag cacatttaaa attaatggac aatatcaagt gattatcggt
     2701 gcaggtgatg ttaatttcgt ttatgacgaa cttatcaaaa aaactggact ttcggaaatt
     2761 tcaacagatg atttaaaaca aattgctagt aaagataaaa aattcaaccc cattatggca
     2821 ttaattaaat tattatcaga tatttttgtt ccaattatac ctgcccttgt tgcaggtggt
     2881 ttattaatgg cattacaaaa ctttttaaca gctgctggac tttttggacc aaaatcaatt
     2941 gaagaaatgt atcctgcgat tgaaggtatt tcctcaatga tccaactgat gtccgctgca
     3001 ccattcatgt tcttaccaat tttagtaggt atttcagctg ctaaacgttt tggagcaaac
     3061 caatttcttg gtgctgcaat tggtatgatc atgacaacac cagatcttgg tggctcaact
     3121 gaatattgga atatttttgg ttatcatgtt tcacaaacaa actatgctta tcaagttatt
     3181 ccagttttag cagctgtttg gatattatct atattagaaa aattctttca taaaaaattg
     3241 ccatcatcta ttgactttac attcacgcca ttattatcag taatgattac tggatttttg
     3301 actttcacag ttattggccc tgtaatgtta ttgttgtcta atggaattac agacattatt
     3361 gtttggctat ataacactac tggatttatt ggaatgggta tttttggagg aacatattca
     3421 cttatagtca tgactggttt acatcaatca tttccagcta ttgaaactca attactttca
     3481 gcgtggacaa atggtatcgg acatggagac tttatctttg tagttgcatc tatggcaaac
     3541 gttgctcaag gtgctgcaac atttgccatt tggttcttaa ctaaaaattc taagacaaaa
     3601 agtcttgctt cctctgcggg tgtctcagca cttcttggaa ttacagagcc tgccttgttc
     3661 ggagttaact taaaataccg ctttccattc ttttgtgctt taattggttc aggaattgct
     3721 acagcagtag caggattact taaagtagtt gctgtttcac ttggttcagc aggtttcttg
     3781 ggatttcttt caattaatgc aacatctatt ccattttacg tattgtgtga attgattagc
     3841 tttgtagttg cttttgcaat tacttacttc tacggtaaaa caaaagctgc tggtatcttt
     3901 aaagcagaag aaattgctga acaaacacca gttaattctt caaaagtagc aacaacagat
     3961 ccaatatcta ctgatgttga atcaacacaa acaaaagttt catcagaaac aattgttagt
     4021 cccatttctg gtaaagtgat tagtctttct tcagtaaatg atccagtctt ttcatcagaa
     4081 gctatgggaa aagggattgc cattaaacca aatggaaaca cggtccattc tccagtagat
     4141 ggtactattc aactcacgtt tgaaactgga catgcttatg gtattaaatc aaataatggt
     4201 gctgaagttc taattcatat tggtattgat actgtatcaa tgggaggaaa aggatttaca
     4261 caaaaagtta ctactaatca aaaagttaga aaaggcgata ttcttggagc atttgaccgt
     4321 tcaatgattg agaaagaagg tcttgatgat acaacaatga ttattgtgac caatacttcg
     4381 gattattcag aagtaaatat agtagctgaa aatacagtca ctgagggtac tgatttactt
     4441 gaaatcaaat aaatatattt ccctgagtaa agatggaatt ttctttccaa aatcaaataa
     4501 tatagattaa tcaaatattt gagaataaca ttttatttaa tcttttcata tatcaattgt
     4561 atgaaaaatt attagaattt taaaaaatga taaaaattca aaaagaaaaa atagtagtta
     4621 tttacttgtc atgttacta
//
LOCUS       DAZKBH010000150         4634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000150 DAZKBH010000000
VERSION     DAZKBH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.152"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..518
                     /locus_tag="ACG8OO_002640"
     CDS             174..518
                     /locus_tag="ACG8OO_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9046474.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ACG8OO_002641"
     CDS             519..938
                     /locus_tag="ACG8OO_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP9046475.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ACG8OO_002642"
     CDS             complement(1030..1380)
                     /locus_tag="ACG8OO_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046476.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2676)
                     /locus_tag="ACG8OO_002643"
                     /pseudo
     CDS             complement(1346..2676)
                     /locus_tag="ACG8OO_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
     gene            2932..3498
                     /locus_tag="ACG8OO_002644"
     CDS             2932..3498
                     /locus_tag="ACG8OO_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9046477.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="ACG8OO_002645"
     tRNA            3643..3730
                     /locus_tag="ACG8OO_002645"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4634
                     /locus_tag="ACG8OO_002646"
     CDS             4064..>4634
                     /locus_tag="ACG8OO_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFP9046478.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaa
//
LOCUS       DAZKBH010000151         4611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000151 DAZKBH010000000
VERSION     DAZKBH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.153"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..400)
                     /locus_tag="ACG8OO_002647"
     CDS             complement(86..400)
                     /locus_tag="ACG8OO_002647"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9046479.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(451..705)
                     /locus_tag="ACG8OO_002648"
     CDS             complement(451..705)
                     /locus_tag="ACG8OO_002648"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9046480.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACG8OO_002649"
     CDS             complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACG8OO_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFP9046481.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="ACG8OO_002650"
     CDS             complement(1145..2098)
                     /locus_tag="ACG8OO_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9046482.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="ACG8OO_002651"
     CDS             2710..4203
                     /locus_tag="ACG8OO_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP9046483.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="ACG8OO_002652"
     CDS             4338..>4611
                     /locus_tag="ACG8OO_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFP9046484.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DAZKBH010000152         4609 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000152 DAZKBH010000000
VERSION     DAZKBH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.154"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(160..771)
                     /locus_tag="ACG8OO_002653"
     CDS             complement(160..771)
                     /locus_tag="ACG8OO_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9046485.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(894..1019)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1101..1817)
                     /locus_tag="ACG8OO_002654"
     CDS             complement(1101..1817)
                     /locus_tag="ACG8OO_002654"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9046486.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1823..2392)
                     /gene="scpB"
                     /locus_tag="ACG8OO_002655"
     CDS             complement(1823..2392)
                     /gene="scpB"
                     /locus_tag="ACG8OO_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFP9046487.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2376..3146)
                     /locus_tag="ACG8OO_002656"
     CDS             complement(2376..3146)
                     /locus_tag="ACG8OO_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFP9046488.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3143..3514)
                     /locus_tag="ACG8OO_002657"
     CDS             complement(3143..3514)
                     /locus_tag="ACG8OO_002657"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9046489.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3533..4420)
                     /gene="xerD"
                     /locus_tag="ACG8OO_002658"
     CDS             complement(3533..4420)
                     /gene="xerD"
                     /locus_tag="ACG8OO_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9046490.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1270 a          994 c          799 g         1546 t
ORIGIN      
        1 ttaaaaggag aagaatttca catttgcaaa gaattgattg atttttatgc ataaatcaaa
       61 aaaatactca aaacaaacaa ataaaacgtt tacattattg catgtcttgt atttttttcc
      121 aaaaaaaaag accaagaaat ttcttgatct ttttgatatt tatttgactg ctgtatgacg
      181 ttgttcgaac tgatgttgtt ttcgacttaa ccatggaaga agttgcgcat gcagcactaa
      241 aaagacagca ctcacgatga atcctttgat caagttgaaa ggaacgatcc caatcagtac
      301 atagtttgct aaagacatgc ccagcatctg tgttgcgttc agccctaaaa acattaagta
      361 aagaggcgta ataacgaaat aatttgcgat actcataaaa atagttagag cgatggttcc
      421 tgtaatgaca cctaaaaatt tgtttcttcc tttatgctgt ttctttacac taaagaaata
      481 ataaatcggg aatgtaaaga tcgttgttga taagaaacta gcagtgtctc ctaccaaatt
      541 atccggagaa aaacctgtag tcagcagatg taatactgaa cgcaagaacg ctgttgcaac
      601 tccggccgct ggaccgaata agaacatgct gatcaatacc gagatatcac taaaatcgat
      661 cttcaaaaaa ctaaaagccg gcatgatcgg aaaagcaaaa tactgtaaaa ctaatgcgat
      721 cgcagcaaac atcgctactg caaccatttt ctgtacacgg gtgttcttca tttgaaatcc
      781 tcctgttagg actcctcttc agcgaacttt ttgacgtttc tctccaaatg aaaaaactct
      841 attttccagg gaaaatagag ttagagttat ttgatcaagc gtcgtcaaat aagctctatc
      901 ttctttatcc agactatact gtcggtatcg gaatttcacc gattcagcta tttttttgca
      961 aaataggtcg tggactataa ccaccggtcg ggaatttcac cctgcccctg aagacgaacc
     1021 ttatataatt tttgtttaca tattcattat acatatctat tcaaaaaacg caacatctaa
     1081 caattataaa aagtaagtgt tcattttgag tggttcagca aactgctgac ttcttttttg
     1141 ttcagttcac ggtaatcccc aggacgtagg ccttgtaaag tcagatctcc gtatttttca
     1201 cgttttagtt tttgcacagg aagaccaact gcttgaagca tatttttcac ttgatgatta
     1261 cgtccttcgt ggatcgtcaa ttccacgata ctgtggtttg tcttaggatc cactgataaa
     1321 atttggaatt tagctggaga tgtttttcgt ccttcgatct ttattccttt tgtcaaaggt
     1381 gcaagcatcg attttgtagc gatccctttt acctttgcaa cgtaaacttt atccacttca
     1441 tgttttggat gtgtcaggcg ctgagcaaaa tctccatcat ttgtcagtag caagatcccc
     1501 gaagtatcgt aatccagtcg acctacagga tagatacgtt cttttaccat cggcaaaaga
     1561 tccgtaacaa cttttcttcc tttgtcatct gcaacagcag aaataactcc tttaggtttg
     1621 tacaataaat agtaaccgta ttcttcttga taaattggga cttcatccac ttccacagtg
     1681 tcatgtttcc caacttttat ccctaattcc cttacaactt gtccgtttac ttttacacgt
     1741 cctgcaagga tgaattcttc tgcttttcga cgagaagtga tgccagcgtg ggcaattact
     1801 ttctgcaatc tctccattac aattattctc ctttattcat tccatcaaag aatagatctg
     1861 gtgacacgtc ttcttcaagt gaaatttcca tttcttgtgt atcaggcagt tcttctaatg
     1921 atttcaaacc aaaataatct aagaagtagc cagttgttcc atataatatc ggccgacctg
     1981 gtccttctac tctacctttt tcttcaatca gtttatgggc agctagacgc tgtaccgctc
     2041 ctgaaacctg aactcctcga atctgctcga tttcagctct tgtgattggc tgtttataag
     2101 cgatgattgc taaggtttcg acggcggctt gagataatgt gtttgatgtc ccttgcgcat
     2161 atcgcttaag taagggagcc agctcttttt tagtcgttaa tacataatgg ctttccgttt
     2221 caaaaatatg caaagctgag tgcgagtttt gagtgtaaga ttcttttaat tctttcacta
     2281 aacttgtgac ttctgcttcg ctgagattta acagtccgct taattcttca ttactgatcc
     2341 cttcctcgcc aacaataaaa agaagggctt caagttcact taatttcgtc atgttcggta
     2401 tctcctactg ctggataaag cataattgat tcatagtttt ctttttgttg cgcaatgatc
     2461 tgcccttttt tcatcaattc tagcaaagcc ataaaagtag tgacaacttc acttttagaa
     2521 ggtgactcaa aaaaagaatc gaaagtacag ccatgcgcat ttctttttat acgctcttcg
     2581 ataaatacca ttcgttcttc gatcgttgtg tcatcgggcg tgatcgtttt ttccactttt
     2641 tgagaaaatt tcttacgttc taagattttc tgcatggcac ggatcaaatc gatttttttg
     2701 acttgtcctc gcttcaacgg cttttcagca tccgctaacc catcgatgtt cataggttct
     2761 tttgtgtaat aaagacttcg ttcctctgct ttttctgtca gtctttcagc tgcatattta
     2821 tatttacggt attctaaaag ctgagtgacc aattcttctc gaggatcttc ttcccataat
     2881 tcttcatcgt ctgtgccgaa ttcttggaca ggaagcaatg tcttgctttt gattgccatc
     2941 aatgtagcag ccatcactaa gtattctcct gcaacagcta gctcgagtgt tttcattgta
     3001 tggatataat tcatatattg ctccgtgatc gtagcgatcg gtatatcata tatatcgatt
     3061 tccatttgct gaatcagatg aagcagcaga tcgagaggac cctcgaatac atctaattta
     3121 atattgatct ccgtcaactc attcactctt tcttcttttt ctttgctagc cattttccaa
     3181 tgtaaggctc tgtgatttca gttgcacgga tggagacatc cgggtacttt tcctgtaatt
     3241 catctaacat ttgaaagccc atattctcac cgcgataaga cggattcaaa gaaatgtcat
     3301 ggatgatcag ctgatcgttc gttgcccagt aaataccaat aatcccttga atgttggttg
     3361 aaccttctgg cgtaaagaaa aaaagttcat aatcgtcttc agattcgtac gtatcaatct
     3421 ccttcaacaa agaagtttta cctgtcagtt tcttatggaa actcaatagt cccatagcaa
     3481 ttttacggtt ttctttccgg taatgtgtta acatcttctc accttcctat gactaagccc
     3541 ttggaaaatg ctgcttataa acatccgtca ttcttttctt cgtgatatgt gtataaatct
     3601 gagtcgtaga gatatccgcg tgacctaaca attcctgtac tgtccgcaaa tcagctccat
     3661 tttctagcag atgtgtggca aagctatgcc gcagcgtatg tggtgttact tctttgtaaa
     3721 tcccagcctc tcgtactaac tgcttcagat ttttccagat tccttgccga gaaagacctt
     3781 tcccatgatt attgacaaat acatgcattt cagatgcgtc ctttacaagt aatggacgtg
     3841 cttcgtctaa gtagcgttcc agccattgga tcgcataatc acctaaagga atgatccgct
     3901 ctttatctcc ttttccaact gtctgtacca gtccaagaga aagatgaaga tctcccaatt
     3961 tcaatccaat caattcactg acgcgcatcc cagtagcata catgacttcc aagattgcac
     4021 gatctcttat ccctaacgtt tttgtcgtat caggtgcttc aatcagcttt tctacttctg
     4081 tcagagtcaa tgtacttggt aatttctgtg ctttcttcgg cgtatcgata tgttgcatcg
     4141 gatcatgatc agtgatacgc tcttgccgta aaaactgatg gaatcttctg agacttgaga
     4201 tcatccgagt aacagttgct gtcgcttttt tttcttcatg caacttctcc agaaaacgga
     4261 tgactaaaaa gcgatcgaca tcctgccatg aagcaatttt ctgctcctgt agaaaatgca
     4321 agtactgttc caaatcacgt tcatagcttt gtcgtgtatt ggcggacaat ccccgttcaa
     4381 tattcaagta gtggagataa tcgttgattt gctctttcat aataatcccc ctcatgcctc
     4441 tctatcatat cagataccgc ttcattctga tagtcttttt ccataatctg agcaaagaaa
     4501 aagccgtcta gacactcaga cggactggtt tcttttttcg gctctataat ttatgggact
     4561 gatgaatcag aattgattct tcagccccat tttcatttta ggtattaaa
//
LOCUS       DAZKBH010000153         4425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000153 DAZKBH010000000
VERSION     DAZKBH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.155"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG8OO_002659"
     CDS             690..1658
                     /locus_tag="ACG8OO_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9046491.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACG8OO_002660"
     CDS             1726..2484
                     /locus_tag="ACG8OO_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFP9046492.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG8OO_002661"
     CDS             2552..3262
                     /locus_tag="ACG8OO_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9046493.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG8OO_002662"
     CDS             3298..>4425
                     /locus_tag="ACG8OO_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9046494.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZKBH010000154         4352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000154 DAZKBH010000000
VERSION     DAZKBH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.156"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACG8OO_002663"
     CDS             complement(<1..1031)
                     /locus_tag="ACG8OO_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9046495.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASL"
     gene            complement(1139..3166)
                     /locus_tag="ACG8OO_002664"
     CDS             complement(1139..3166)
                     /locus_tag="ACG8OO_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFP9046496.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(3311..4039)
                     /locus_tag="ACG8OO_002665"
     CDS             complement(3311..4039)
                     /locus_tag="ACG8OO_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9046497.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1213 a          903 c          713 g         1523 t
ORIGIN      
        1 aaaagtgaag ctactaagat ataaatccaa ttattgtctt ttttaatcgt aacatccgtc
       61 gtgttcaatt ccttggctac ttttccgtct attgtaaaat ctttttcaaa tacccattga
      121 ttggtatagt tgatcgtagt atttcctttt tttctcgaaa attttccatt ttcattttca
      181 tttcctaaaa cagtcattgt aaggtgatat tcacctgggg ttagcggttg accatttaaa
      241 gcagttggaa aagaaaaatt tgtattcggc gcaatctgca atccttcttt ttcctcttga
      301 tataaaactt cagaatgtcc tttttttgta atttttgttt caataactac ttgattgata
      361 taggtttttt gatcattttg aacgtttgct aagatgacat ttcttgcatt gatttgatct
      421 ggtttaactt catgaagagt aaggttcgga gcaacttcgt tcagtgtctg ttgcatcaac
      481 aaagcaataa tataggtgta ttcattctca atcgataagc tttgatcctt agtttctgtt
      541 tggtcctttt ccgtctggat ttcttggaat gtgatcccac cagccaatac accatcaaat
      601 cgctcattcg gcatcctgac atgaaaagca acggtttgga cacttttagg ttttagcgtc
      661 actatctgtg gatagctcac ataatctacc agatcaaaga tcaggctcat gtctttttca
      721 atctcgtttt ttccgtattc aaccatcaca tttgaattgg tggttgcgct attcacagaa
      781 atatcgattt tcacttcttt ttctgtatca tttctcaatt ccgcttttac ttctacttcc
      841 tgatccggat caagttgaag atcgaagtaa gtttttctct tgtcgatttg attctctgaa
      901 gttatcggcg tcactgaaaa attaaatgca ctagctgaca ctgatatatt gttaaaaagt
      961 gcccctaaaa taactaggag tgttataaat aattgttgtt ttggtctcaa gaaaatcgct
     1021 cctttaatca tatgtaagag aagactataa caaaatcttg tctctgtggg acacaagagc
     1081 ctatggtacc tgtgcccttc ttgaaactca ataattttgt tatattcttc tcttcctgtt
     1141 aatttcctgg gacatctgca agtatccaag atagtttccc ctcaaaggtt ccgattcttt
     1201 ggttttctac aggaatatct attctgattc cacgatgagc tgtagaatct aaaagagagg
     1261 acaaatcgct ttcacccttt tgatcacttt cgaaaatcgt aacagcggat gtccctataa
     1321 ccgttgtatt ttcagaggta acaaaggaaa gagcttctgg aagctcaaaa tctccatttg
     1381 taagaacttt ctcttgtttc aattgtaatt tccaacttcc aaaagcccgt tcatctataa
     1441 ttttcaaccc acctgaaaac tgtccgaatt cacttaatgg tctactggat attttatgtt
     1501 ttccaaaatt gacatattga ggagcagttg agagataaag tttgcctttt ctgtaataaa
     1561 aatagatatc ctctgtattt tcggagaaga tgccttctgt attcgctgaa tccgagtcaa
     1621 aaacataccc ttcaatatca ataggagttt ctttatactt ctctcctatt ttcccagtga
     1681 ttgttttcat ttcagcaata tcaatctgac tgtctttatc tttataataa acatggatat
     1741 ctatctcctt cagctttgcg atctttaggt ttatttcatt cgattgctca atcaaattcc
     1801 cgttttcaag gtattcaaaa tgcatagtga gtactttttc aggaattact tcctgcaaag
     1861 ttgtctccaa ttcgattatt aacgtatctc cagcaataaa ataatctgtc agatttagcg
     1921 tgactcgatt tccggttatc aagatatctt tggcatcaat ttgatttcca ttgatcaaga
     1981 tatgctcggg aatagttaat tcttgtggga ggtcgatact agtctgcatt ttcttatatt
     2041 caagaaaatc cccctcgacc ttcaatgtat ttctgatagt tatcttatcg tttcctccag
     2101 cttcagcgat ggccccataa tcactatcgt tgacttttac atcagctgtt gatgtgacat
     2161 tcgatggaag tcgcgtgaca gcgacagcat tttcttgaaa aaaagtacct gttgaagaag
     2221 taaaccccca atttacttct gtagaaccaa actgcctaat gagatcaggt ataatccgag
     2281 aagagtgacc gacaccttcc aaatcataag taagacttct ttgctccgcg tcccatttaa
     2341 ttattaacaa gcgccatgtg ccattagaaa gaaatgtagg agctaccgtc acgccagtat
     2401 gctctccaga ataattatta ttatttggtt taggcgtgac aaaagcgaca tgtccatgcc
     2461 ctctattatc agcagttatc tctcgatcca ttcgatcaga agaaccacta tttttatacg
     2521 tatcaaactc aatcgagagc gcattcgcaa tgtaaggttg gccagaacga gtagaatatg
     2581 agccaattcc catacctggc gatccaataa cttgactggg attactagcc attctaggat
     2641 cgccagtcaa tgtaaaggtc atgccatctc ccgctctatc tcgttgattt cctaagtata
     2701 aataagattt aaatgtaaaa tccgagagta aactaatctt cttatttgac caaatagctc
     2761 ctctttgaga cctttctgct gggttcaact gcagtaactt cccattttcc aatatggacg
     2821 tgctagtacc gataggtgtt tgaaaaattt tatcgataga aataatattg ggatcgtcag
     2881 gcagctcaac cgcagaaatt ggtgcatctt ctagtccttg tttgtccgaa ccctcagctt
     2941 ctacgttgtc agtggtctct ccactactcg aaatacttaa atcctcactg ctttcttgtg
     3001 tctggttgga ttctattaaa ctgtcgaatg tcgaagtgag tttgctttct tcgtctcttc
     3061 tatttccatg ctccaactga ttcaattcca aagagtaccc tatgatcgga aagatattag
     3121 tcactaagag actgcttaaa atgagcacga tcctcctctt tttcaatata tcttttcctc
     3181 ctgatcgtta aattcctaga tgaactattc gtatccaaac aaatttagcc tagcctttga
     3241 caatttttgc tcgattacct cgcaagacaa tatgactacg tatttatttt taacagttct
     3301 tttcagtaaa ctagttgtta ggtgtatctt ctaatgtcca aactaattta gtggaatatt
     3361 gttgcgctaa cttgacagat ttaccgggta ccgaaagctg taccgcatct tgattttcat
     3421 tttcatttgc accaaatcga tagacccaag tccccacacc tgttcctgat ttagcattaa
     3481 ttgctaacgt attgtttcct cccggtgtca acgttaattc agaattcacc gtttgcggcg
     3541 tatatgttgg atcaaccaaa gaaacagcct gccctttcgt aaatttcagc tgtgctccaa
     3601 ctagttcatc cccactggca gttttaaact gttctggttg agagacactt aatttccacc
     3661 cagataaggt acttctccta tctgtcactt ggacatatgt cggttttcta gacccatcgt
     3721 tcatcacatc agcagctgca taatacgttt tatccgcagt agaaattttt tgagtcccaa
     3781 atttgaattt tgatccataa tcaatcgata atgcccctcc tgttccaggg cttggaggat
     3841 ctgatggatc gacaggttta ttgggatcag gattctctgg atcaacagga ggagttacac
     3901 cctcatctcc tgcttcaaat gtaatcgttc cttctgtgtt atattccttt gggtatactt
     3961 cagttgcaga aacaaaagtc gtacttgcta ccatgctgaa taacatcatt gctgctgtta
     4021 atacgatttc ttttttcatc attgacattc ctctttcttt atttactaaa catatcagtc
     4081 gattggatta atccatagca aaattctgtt ttacgatact tatgaaataa aagaacatag
     4141 acctatattt ggtcctatgt atcgattagt tataaatgat tatcagcccg atttgatttc
     4201 tagtttttat tgcaacttga tgcaaacaca atctagaaat acagctgtca tagcctttaa
     4261 aataattcgt aagtccccct cctatctatc aaaatgtgac ttgactgcct agacttcaat
     4321 caatattaat tataattaaa taaaaaatgt at
//
LOCUS       DAZKBH010000155         4345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000155 DAZKBH010000000
VERSION     DAZKBH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.157"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1853)
                     /locus_tag="ACG8OO_002666"
     CDS             complement(387..1853)
                     /locus_tag="ACG8OO_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9046498.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /locus_tag="ACG8OO_002667"
     CDS             complement(2144..4228)
                     /locus_tag="ACG8OO_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9046499.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DAZKBH010000156         4324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000156 DAZKBH010000000
VERSION     DAZKBH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.158"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACG8OO_002668"
     CDS             complement(<1..281)
                     /locus_tag="ACG8OO_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9046500.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="ACG8OO_002669"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="ACG8OO_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="ACG8OO_002670"
     CDS             complement(2359..2721)
                     /locus_tag="ACG8OO_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046501.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="ACG8OO_002671"
     CDS             complement(2725..3033)
                     /locus_tag="ACG8OO_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046502.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="ACG8OO_002672"
     CDS             complement(3042..3461)
                     /locus_tag="ACG8OO_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046503.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="ACG8OO_002673"
     CDS             complement(3454..3687)
                     /locus_tag="ACG8OO_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046504.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="ACG8OO_002674"
     CDS             complement(3684..>4324)
                     /locus_tag="ACG8OO_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9046505.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DAZKBH010000157         4284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000157 DAZKBH010000000
VERSION     DAZKBH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.159"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(394..1899)
                     /locus_tag="ACG8OO_002675"
     CDS             complement(394..1899)
                     /locus_tag="ACG8OO_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9046506.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1896..2582)
                     /locus_tag="ACG8OO_002676"
     CDS             complement(1896..2582)
                     /locus_tag="ACG8OO_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9046507.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2775..4196)
                     /gene="gndA"
                     /locus_tag="ACG8OO_002677"
     CDS             complement(2775..4196)
                     /gene="gndA"
                     /locus_tag="ACG8OO_002677"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9046508.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1200 a          892 c          714 g         1478 t
ORIGIN      
        1 cgaagttttt ttcgataaaa tcaagcttta ttagctatct catccaaacg acttcgttat
       61 tataacatga catcaaaagt gcgtcaacac tattttttat aatttttagt gacaccttaa
      121 aggaatcatt tagaacaaat aataatatag caatctcttt ataaaattgc aatagaaaat
      181 tccctatttt tatttattat ttttaaacac gaatattaca gcattttatt gttttttcgt
      241 tcatctaaaa gatacttaca tttaattaac ggatgttatt ctatttaatt gttcgctagt
      301 ttttttataa tgacatccct gaatttctca cttgcttgta ttttgtacct tattctttgt
      361 tggatatata aaaaaacaag taaagcacat gctttacttg tcttctgcct ttgcgatagg
      421 aataaagatt ctaaaaattg tcccttgccc tactacactt tctgctaaga tacgtccttt
      481 atagttttca accagttgtt tagcgataga caaccccaga ccatttccac ccttcgttct
      541 agctcttgct ttatccactc gataaaaacg attgaagatc ttattaaggt cttctttggg
      601 tattccttcg ccaaagtcct gaatcgcgat ctcaaattca ttcagagtgg aagaaataga
      661 gatatggact tcttttcgtg tagttgaata ctttactgcg ttatccaata tgataatgat
      721 taattgttca aagtgattac gataaatttg cagggtcact tcttttgtca aatcatcatc
      781 taaagtgatc acatagtctg gatataatat cttgaaatta ttataaactt gataggtgac
      841 ttcttttcct ttagttgttt cattcgcata atgaacatct acttgttctg ctcttgataa
      901 gtctagcatc tcttgaacta agcttttcat acggctgatt tcttgcatgc tggcgcttaa
      961 tgattcttct aatatctcag gatcatcttt cccccaacga tttagcagac taagatgccc
     1021 ttcaataatt gcaacaggtg tccttaactc atgagatacg tcttccacga attgctcttg
     1081 ttgttcaata tacaaacgca tacggtcaag catctcattg aatatttcag ctaaatctgc
     1141 caattcatca tttgtgtcga tttcaggcat gtggatatct gattgcggat ctttacgtat
     1201 agtatccatt gtatctctga gtaccttgag tggttttaag aaataagaag ataaaaagaa
     1261 acctaaaata ctacttaaga tcaaagaaac aacttctaaa acaataagag tcaataatag
     1321 cttatttcga atatcataga aagaagataa ttcatagaag ctttgcgcat agccgatctt
     1381 ttctcttgta cctttagaat atataggctg gatagataaa aagccagttt ttccttctag
     1441 tgtaacgatc gttggaactt tacgtgtagt ctgaatcagg tttcgactgt tttcatgggt
     1501 tttaaaaatc aagttctcgt ccaaatcata aacataaaga tccattgatg gctgccctag
     1561 ctcagctata aaggaatcga tcttcattaa gtttccttcg accagtgaag tatgatctac
     1621 gacctcatct ccgtcatttg tcgctttcgt cagattacga tacgtattta ctaatgtcag
     1681 ttcttcatcc gagttagcca aacgagaagc tacttctgaa atcgtacgct ctacattgtt
     1741 tctttctttt gccacaatca gattgataga tgatttatag gtaatgacag cgaaaattgt
     1801 aaacacaacg aatatgaaga aagaactcgc aaaggcccac ttgattgtca aggagggccc
     1861 tttcagttct cttttttccg attttttcgg ctttatcacg agcgcatcac ataacctgtt
     1921 ccgcgtacag tttggatata gctttcctcc ccgggaacgt caattttgtt gcgaagataa
     1981 cgaatatata cgtccactac gtttgtttca acttctgttt cgtatcccca cactttattt
     2041 agtaaaacgt cacgtgctaa taccacgttc acgttttcca ttaaagtaag cagcaactcg
     2101 tattctcttt ttgttagttc aattacttca tttccgcgac gtacgacacg gttttctttt
     2161 tcgatagtca aatcgcgata agtaatagta gtttgtttag ccacattttt atcgccttca
     2221 atatcgattc gacgcaacaa tgcacgaaga cgtgctaata attcttcgat agcaaatggt
     2281 ttgacgatgt agtcatctgc tccgtggtca agtccagata cacgatcgat cacagaatct
     2341 cttgcagtca tcataatgat tggtgtattt ttgacttggc gtacacgtct gcagacttct
     2401 aaaccgttca attctgggag catcagatcc aacaatatgg catcccactc attatttaat
     2461 gccgcatcta aaccagttcg gccgttgtag tggacttctg cgttataccc ttcatgcttc
     2521 aactctagct cgacaaatcg tgccaagttc ttttcatctt caatgattag aatattgctc
     2581 atttattatt ttatcctttc tattctactg gctttttaaa aactctatca ttataccgat
     2641 aatatgctta aaaatctata gtcattttat catggatagc aattttaaaa aagagctgaa
     2701 gtctactcat aacaatcgac ttcagctctt tcgtcctctt tttattttct catttcctaa
     2761 cagccgaaag actattattc ttcatggtac catgagtaat ggaagattcc ttctttgtcg
     2821 acacgctcat aggtatgtgc gccaaagtaa tcgcgttggg cttggattat atttgcaggc
     2881 agacgttctg ctctgtatga atcgaagtaa gcgatggctg atgagaaagt cggtacaggt
     2941 acgccagctt ggacagccaa ggcaacgacg tctctgactg attgctgata ttttttcgtg
     3001 atttcaacga agtaatcatc tagcaataaa ttttcgatct caggattttt gtcataggca
     3061 tccgtaattt tttgcaagaa tcgagctcgg atgatacatc cagcgcgcca gatttttgcg
     3121 atttcaccaa atggcaagtc ccaatcaaat tcttttgaag cgacacgtaa ttgagcaaat
     3181 ccttgggcat aactcatgat tttactgaaa tacaacgctt ctcggatttt ttcgaccaat
     3241 tcttttttgt ctcccgtgaa ctcaaaatcg ttcgtacgag acaaaatctt gcttgcttgt
     3301 acgcgttctt ctttataagc agaaatatag cgggcaaata cagattcagt gatcaatgga
     3361 agtggcacac ctaagtcaag cgcgctttgg cttgtccatt tacctgttcc tttattccca
     3421 gctgcatcaa ggatcacatc tactactggt ttacctgttc cttcatcgtc tttacgtgtc
     3481 agaatgtcag cagtgatttc tatcaaatag ctgtctaatt cgccttgatt ccattcttta
     3541 aagatatcag ccatttcttc tactgaaagg tttaaaatat tcttcattaa gtcataagat
     3601 tcagcaatca gttgcatatc tccatactca ataccgttgt gtaccatttt cacataatgt
     3661 ccggctccat ttgggccaat atatgtgaca catggtgctc cgtcttctgc ttttgcagaa
     3721 atttgctcta agattggtgc aactagcgca taggcttctt tttgtccacc aggcatgatt
     3781 gaaggtcctt ttaaagctcc ttcttcacca ccggaaacgc ctgtaccgat aaagttgatg
     3841 ccagagttgg ctaactcttc attacgacgc atcgtatctt taaagaatgt atttcctccg
     3901 tcaatcaaaa tatcgccttt atcaaggtga ggaagtaact cttggattgt tgcatctgtt
     3961 gccggtcctg ctttgaccat tagcaagatc cgacgtggtt tctcgataga ttctacaaat
     4021 tcttcgattg tatatgttgc tttgaaattt ttatctgggt gttcttctac tacagcggtt
     4081 gttttagccc ctgtacgatt gaataaagcc actgaatagc cgcggctttc tatgttcaag
     4141 gctagatttt tacccataac ggccataccg acaacgccga tttgttgttt tgacatatga
     4201 actcctccta catacgggat ttctccctaa tctcttacat tatagcttaa cacggctaca
     4261 ttgcaaacta aagaacaaca tatt
//
LOCUS       DAZKBH010000158         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000158 DAZKBH010000000
VERSION     DAZKBH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.160"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG8OO_002678"
     CDS             complement(37..1257)
                     /locus_tag="ACG8OO_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9046509.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG8OO_002679"
     CDS             complement(1277..1888)
                     /locus_tag="ACG8OO_002679"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9046510.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG8OO_002680"
     CDS             complement(1931..2863)
                     /locus_tag="ACG8OO_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFP9046511.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG8OO_002681"
     CDS             complement(3284..4144)
                     /locus_tag="ACG8OO_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9046512.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZKBH010000159         4202 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000159 DAZKBH010000000
VERSION     DAZKBH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.161"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..808
                     /locus_tag="ACG8OO_002682"
     CDS             <1..808
                     /locus_tag="ACG8OO_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFP9046513.1"
                     /translation="VHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPE
                     IYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQ
                     TSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPL
                     ISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKI
                     MLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            825..1259
                     /locus_tag="ACG8OO_002683"
     CDS             825..1259
                     /locus_tag="ACG8OO_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046514.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1876..>4202
                     /gene="esp"
                     /locus_tag="ACG8OO_002684"
     CDS             1876..>4202
                     /gene="esp"
                     /locus_tag="ACG8OO_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFP9046515.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1548 a          584 c          790 g         1280 t
ORIGIN      
        1 agttcattat ttttttactg gaaaaataga agatctattc catgaaccgt taattgaata
       61 tacagaaaat ctaagtaaaa ctatcgagca aatcaaaata gataatctgt tatcacaaaa
      121 ttacgatcca gaaatatatc tgtttcttta tgaaaataaa atactggaat atgttaaaaa
      181 tggagatatt cgaaatttag aaaacatggt ttttaatctg agtaatggaa taattccaag
      241 tgttagtgga gatacgattc ggtctgaaaa aaattattct atcattgttt ttgaaaagtt
      301 agcacaaacg tctataacat taggaatgga tataatagaa gcgtatcaaa gtagagatgc
      361 tttgatacaa gaaaacgaat tagctgtatc gttacctgag gtcttaaaag tgagggattc
      421 tgggattgtt tattatacta aggaaatcgg aaaaactaag atagagcatc tttctccctt
      481 aatttcttct gtcgttcaat tcatcggctt aaatatctat aaaagaatta ctgtaaagga
      541 aatagctaat tatttttctg taagtgaaac taaattaaga gaaagcttta agaatgaaat
      601 gcatattacg atttataatt atatctctaa gcgaaaaatt tctacggcta aaataatgtt
      661 gaaatcaaac catactatca gtgaagtttc tttaggttta ggattttcag attcttcaca
      721 cttttcgaga gtattcaaaa agtacgcagg tgtatctcct aagcaatatc aattaggttt
      781 agtggacaat ttcaacaaca ttagctgaga ggttttattt tattatgaga ctatatcatt
      841 tacgtagttt ggaggagttg aggaaattgg attttaacaa aaaatttttt tgggaaattt
      901 ctgatttgag ttgtttcgta gaatctgagc gtctaagtaa tggtaataat agtgtgttaa
      961 atgaatatga caaagtagta attaataaag taaaaagttt atgtgaagaa gctagagaat
     1021 ctatcagtca tattaatggt agcaatttta aacaaacatt atttaatctg attaagctag
     1081 atgctaaaat atcaagttat ttattctttt tgatacatga tgaatttatg gattatcaaa
     1141 aattggatca gttaattgaa agagaaagct gggaagatta ttacgtgggt ctaacttttt
     1201 cagaaaagtt aaacaactac aagttaatag attataagta tttgagtgaa agcaattaat
     1261 tattcatgta gagtactaga cactttaaat atggacaatt caattataaa gaaaaagaat
     1321 caataattta atgaggctgt gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa
     1381 aatgttgata actccaatca ttcgtttaag attctaggaa tcattcagaa atgctgtgaa
     1441 accgatactt tataattata aagaaggtga tttcaaattc cgaagaagga atttgaagtc
     1501 acctttttac ttatgtcaca gtctcattca gtaatgcggg ataaggctcc ttattccgtt
     1561 atttgaatac tctgaagttt aatctaagaa agggtaatat tttgaaaaca tgcttcatcg
     1621 catagaaatg aacttaattt cagaaagaat acatacatat ctttttatat gttttcagct
     1681 gctgaatggt taaaaagcac aaatatccga aaaaaacatg aagaaatgta ctttttttgt
     1741 tgtaatgttc atgaaattct ctaagtaaat tagttaacat aaggttagta agattcctat
     1801 tctgtgaata atacgtatca gatttatctt ttattcttac taggaaagta attattaagg
     1861 gagttgattt gaataatggt tagcaagaat aataagagag tatttctaga aaaaacaaag
     1921 aaacgagtat tgaagtacag tatcaaaaaa ttaagtgttg gggttgcatc tgttttgatt
     1981 ggtgttggtc ttgtcttggg aacaactgaa ctagttaaag cacaagatga aatttctcca
     2041 agcagcccac ttgaaacagc gataagctcc gtacaagtag gtgacaaagt agcttcgggg
     2101 aatacttttc aggagaatcc tggttatacg aaaaattata acttttcgga tttacaattt
     2161 agtcctcaag aactaactgg agacacacta aaaggcaata caattggatt tgaagtttac
     2221 ggaaaacata atattgcagc atcaactaaa aactgggaaa tccgtcttca attagatgaa
     2281 cgattagcta aatatgtaga aaaaattcag gtcgatccaa agaaaggaat tggatcttct
     2341 agacggacct ttgtaagaat taatgattcg ctgggtagac ctacaaatat ttggaaggtc
     2401 aattatattc gtgcaagtga tggattattt gctggggcag aaacaaccga tacgcaaacc
     2461 gctcctaatg gggtaattac gtttgaaaag agcctagatg aaatttttaa agagataggc
     2521 atagacaatc tcaagaccga tcgtttaatg tatcgaatct acttggtaag tcatcaagac
     2581 gatgataaaa ttgtaccggg tattgatagt acgggctact ttttaacaga ttcagatgat
     2641 ttctataata gcttagatgt atctgaaaat aatcctgacc agtttaaaca tggatcagta
     2701 agtgcaaaat atgagaaacc taatactcaa acaaaagatg gttcaggatc cacaggtgct
     2761 aatggagcta taatcttaga tcataagtta acgaaaaatt ataatttctc atattcatct
     2821 tccgcaaagg gcacgccttg gtatgctaat tacaaaattg atgaacgatt agttccatat
     2881 gtagcgggta tacagatgca catggttcag gctgacaaag taacatatga tgtttcgttt
     2941 gaatccggaa caaaagtagc tgatttagca atcgagcgac gggaaggtca tgaaaattat
     3001 ggcataggtt caataactaa taatgattta actaaactta tcgattttgc taatgcaagt
     3061 ccacgtccag tcgttattcg atatgtttta caattgacta agccattaga tgaaatctta
     3121 gaagatatga aagcaacagc acaagttgaa gaaaataaac catttggtga agatttcatc
     3181 tttgattctt ggttgtcgga tacgaataaa aaattaatcc agaacactta tggaacaggt
     3241 tattattatt tgcaagatat tgatggtgat ggaaaccctg acgataaaga agagagcgga
     3301 gacacgaatc catatatcgg gaaacctgaa ttagaagaag tatatgatgt tgacacaaca
     3361 gttaagggga aagtattcat ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt
     3421 gataaagaag gtactgtatt agcagaaaaa actatcgctc caaatgaaaa agatggggct
     3481 ccaatttcag atactgtaga atttgaattt acgggtgtag attcaagtaa attaatcgcg
     3541 aaagatgaat taaaaatcca aatcgtttct ccaggttttg ataaaccaga agaaggttca
     3601 accgttatta aggaatcacc aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa
     3661 ccagatgcta aagaatcaat tcggaataat aaaaacttac ctgaagatgc agagtattca
     3721 tggaaaacag agcctgatac ttctaacgtt actgatagta cgaaaggtat tgtaactgtt
     3781 aagatcggaa atcgaacttt cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct
     3841 atggaaaatg atgcaacata cgtacctata acaacaactc cagaaacgac agttcaaagt
     3901 ggaaaaccta catttgataa accagatgtt cctttagcta aagatgcctt ttcaatttca
     3961 gatgtctata ataagtactt cggtaatgca agtgttgatg caaacactgg tattgttaca
     4021 ttcactccag ctaaaggtgt aggagaatcg gagccgatta ctggaacaat tcctattaaa
     4081 attgtttacc aagatggttc tgtaggcacg accgatttag cagtaactgt aagtaaagat
     4141 atttatgaaa atccaggaga aaacattcct gcaggctacc acaaagtaac cttcaccgca
     4201 gg
//
LOCUS       DAZKBH010000160         4045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000160 DAZKBH010000000
VERSION     DAZKBH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.164"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..1060
                     /locus_tag="ACG8OO_002685"
     CDS             149..1060
                     /locus_tag="ACG8OO_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFP9046516.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1547..1813
                     /locus_tag="ACG8OO_002686"
     CDS             1547..1813
                     /locus_tag="ACG8OO_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046517.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            1885..2226
                     /locus_tag="ACG8OO_002687"
     CDS             1885..2226
                     /locus_tag="ACG8OO_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046518.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2281..2700
                     /gene="ssb"
                     /locus_tag="ACG8OO_002688"
     CDS             2281..2700
                     /gene="ssb"
                     /locus_tag="ACG8OO_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9046519.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            2777..3073
                     /locus_tag="ACG8OO_002689"
     CDS             2777..3073
                     /locus_tag="ACG8OO_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046520.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3088..3519
                     /locus_tag="ACG8OO_002690"
     CDS             3088..3519
                     /locus_tag="ACG8OO_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFP9046521.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            3824..4006
                     /locus_tag="ACG8OO_002691"
     CDS             3824..4006
                     /locus_tag="ACG8OO_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046522.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
BASE COUNT         1490 a          609 c          858 g         1088 t
ORIGIN      
        1 tggagtaaaa caagtgtaga ggaaacttga agacttgttc gaacacaaac ttacaagaga
       61 ggggagtaaa gcagttgcag aggaatcagg aagatttgtt cgaacagaaa cttgcaagaa
      121 agcattatta aattgaaggt gatgaaatat gaagtattta ttgcatggct attttattga
      181 ttattctact cgcaatagta gtactttagc gattctatgt ttgcttcatg aattgagagt
      241 ggttactaga aaacagctat acaaactatt gaattttgac agatcaatta gtaaacaagg
      301 agtaacgaat gctttagtga gattatatga aaaacagtta atcgatcgaa aaaaatatgg
      361 aacagaaatg tattattatt tgacgaaaga gggacatcaa agcataggtg ggtattatac
      421 gttgccgaaa gttcctgaat ataatttgaa tcaccattta caaattaaca attatttgat
      481 taaaggaatg gcattggcaa aagaccaccc acatttaaaa cggattattt cagaaagacg
      541 taccgtctat gaaacgaagg atttaaatcc taagacaaaa ggaagaaaat tttttgtgcc
      601 agattttatt tttcgttttc gagacaaaga aaatcatgag atcaattggt cctttgaaat
      661 tgagctaacg ttgaaaacac gtcgtcgcta ccgtgaagcg atttttccga aatatattcg
      721 tgagttgcga acacgtcctg aatcacattt aatttatgtg acacctagtg gcgcaattca
      781 agaagaactt catgcgttta agcgtatttt tttcaagcgc gaaactgaaa aagatgcgag
      841 tcaacaagaa ttgtttgagc ggttgcatat tttttcagct ttagagtttg aagagggatt
      901 aaaagcatta ttagagaatg atccagcagt caattggcgg acagtagaac gagaaaaaat
      961 ttatccgaat ggcgctgttc aaagtcgttt gaatttattt gaagaatcaa ctgaaaatga
     1021 ggaaaaacca aaagtgaagt ttacgcctga agtattgtaa aagagggtgt tcgcttcgct
     1081 ctttttttgt gccagtttgc tggcaccgtt cagacgcaag cgtctgaacc ggctcgtttt
     1141 taggaaggac taacttgttt gatttatccg ttgtcaaaga agtggacgtt cgagagtccc
     1201 taatcgtata gcagtcattc ggcgagtata acctaaaaca taacctgcca attgccaagg
     1261 gtaaaatgca cgctgcgcgc ccttggcaat cgtcagaaca tgttttcgag ccggttatgc
     1321 cgaacgaccg ctatacgaaa agggcctcta aacagaagtc cactttgatc cttgacaact
     1381 gaaacccaaa atcaaacaag caggccttcg tctaaaaacg agccgaatga agaccttggg
     1441 ggttgcaccc ccaaaccccc gtgcccccga cacactaccg aaaaggtggg ggtgggtggc
     1501 gaaaaaatca aataaaacat tctattcaag aaaggaagaa acaaccatga caactaaaaa
     1561 cgagcaactg aaagaacagg caagaaaaga gttgcaagaa agaggtttga ttattgaggg
     1621 gagctttgaa ggagattttg aaacctatat tggttgttat gcaagaccga taaacaaacc
     1681 tacagcctta gacccaatga acgaacaaga agtaaaagaa caagaaaaac atgcagtgaa
     1741 tggttttccg caagatttca ctgaatggta tgagtgggaa attaacaacg ggaagcttga
     1801 aaacttctta taaaagtcag atgacgtgca aagagaatga ttctctttgt acggtaaaaa
     1861 tataaagaaa gaaggaaaaa gaaaatggca aaaaacacac cttatcaagt aggagacatt
     1921 ttttatagta gttggggcta cgaacaaacg aacgtgactt tttggcaaat tgtgaagtta
     1981 acagaaaaaa cagcatggtt tcagcctatt caaaagaaaa cggtcgagca ttattcgtcc
     2041 atgagtggaa aagttgttcc aatgaaagac aagtttatag agtatccttt agcaaaaact
     2101 aaagacagta ttgtaaaaaa acgaattgat ccagaaacct atcctgatat gttatgtggt
     2161 tatcgtagtt gggacacgtt tcatagatat aaaaatcaag ctgtttatga aagtagttat
     2221 tattaagggg atagtttccc ctttcaaaaa taataaatag aagaatggag agaaaaaatc
     2281 atgatgaatt cagtgaattt agtaggacgg ttaacaaaag aagtggattt aaaatacaca
     2341 ccaaaaggca atgcgacagg cacatttatt ttagctgtta atcgaaatta tacgaatgca
     2401 aacggcgagc gtgaagcaga ttatatccgc tgtgtcattt ggcggaaagc tgcggaaact
     2461 ttagcaaaat atactggaaa aggaagtttg attggcatta atggacgtct tcaaacgagg
     2521 agctatcaga atcagcaagg acagaccatg tatgttacgg aagttctagt gacagatttc
     2581 tatttgttag agagtaaaga aatcaatgag caacggtcaa aatcaatggc gaataatcaa
     2641 aataattctt caattccagg aaatgagatt caaatttcag atagttattt gcccttttaa
     2701 gtgaaaaata attttgttct atacctgatt tcttcaggta tagaacaaaa aataaataga
     2761 gatgaggcga ttttttatga aaatcattga cagttacgat caacgaacca tgcaatattt
     2821 gggctcgttt gattatacag atgaaaatat tattaattat gttgcaagtt tacctcctta
     2881 tcaaagcgtt cgcttggtag attttgcaag tgatgaattg gtattaacaa cgatgggcaa
     2941 ctttttagat tatgtcggag acaaaaaatg gttggaaaaa attttgtcta aattgattgc
     3001 gaaacaaaca ggagaagaac ccataaatgc gattcaattg ttcaatcgat atgaacaaga
     3061 aaacaatgat taaagaggag agaaaaaatg aaaagattaa aacgaagtat ttttattgtg
     3121 ctagcattga ttgattttgt tgggacaggt tgtgaagcaa ttgacgttca aaaagctccc
     3181 ttatcggaaa taaaacaagc aataacaaaa caagatgaag aacaaccgct aaatacacaa
     3241 gtagaaatag tgaatcaaaa taaaccgagt ttttctgaag aagatttttc gttagaaaat
     3301 ggaaccaggc aatttttttc agatttggat cgtttaaatc gggtaggtgt agcaagtgca
     3361 cttttgcata aaagcaggat gccaacagaa gaacgagacc gattatacat taagccaact
     3421 ggctggaaac aaaagaaaat gaagaatggc gattatcttt ataatcgttg tcatttgatt
     3481 ggctatcaac gtgcgacaag tttccccata agtatgtaat acgaaacggg aggattaatc
     3541 ttaagattaa tcacaactaa cctatttgac agttggaatg aaagctatca tgtctgctga
     3601 atcaactggt ctgatactcc tacatgcaat aaagtctact aggaagttat tgtatgaagc
     3661 taggtaaagg cggcagaaca atttctaagt gtggaatcat atgaagttgg atatgccggg
     3721 acgctataaa ttactcaggg tgagactgtt tttaattaaa ctgacgaact tgcgaatgta
     3781 cgggtctata accgacttat attcgaaaga tataagattg cgaatgcaaa gaacattatc
     3841 gaaaagtaag aatgttggtc atgaaattcg agatggaaga ctagcggtgt atctgtcaag
     3901 agttggtaaa acatgtttga ttgattcaga tgagagtggg aatgcgacaa aacgttttac
     3961 agaagaaata gagcaactag cagaactgaa tatacgatat ttttaaatac tttagcggag
     4021 aataaaaaaa tcctctacag aaaac
//
LOCUS       DAZKBH010000161         4026 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000161 DAZKBH010000000
VERSION     DAZKBH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.165"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(232..381)
                     /locus_tag="ACG8OO_002692"
     CDS             complement(232..381)
                     /locus_tag="ACG8OO_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046523.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            843..1055
                     /locus_tag="ACG8OO_002693"
     CDS             843..1055
                     /locus_tag="ACG8OO_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046524.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1333..3132)
                     /locus_tag="ACG8OO_002694"
     CDS             complement(1333..3132)
                     /locus_tag="ACG8OO_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFP9046525.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3256..3852)
                     /locus_tag="ACG8OO_002695"
     CDS             complement(3256..3852)
                     /locus_tag="ACG8OO_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9046526.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1443 a          711 c          689 g         1183 t
ORIGIN      
        1 ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt cataaaaaac
       61 gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag agagacaatt
      121 tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt acaaacgtct
      181 tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc cttatctaga
      241 ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt tattttcgtt
      301 ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc ggtattgcat
      361 atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta gttcttgaaa
      421 gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt tgatgcaatt
      481 aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct attttctttt
      541 aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat ataaatcata
      601 tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga acaacatagg
      661 aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata gttatctaaa
      721 aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt tcaaatacaa
      781 ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa agagggaatg
      841 aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga gtatctcatg
      901 aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat gttgaaagtt
      961 atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc gtaaaattct
     1021 gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga attcgcaaga
     1081 actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa aaagaaaaag
     1141 ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta ttttagctat
     1201 tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg tcaaacaaaa
     1261 aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac ttttttcatt
     1321 aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg aataagtgat
     1381 ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg ttatgatacc
     1441 atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg tccatacata
     1501 tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg tactatattc
     1561 cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag gtaagatgta
     1621 gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt acgtcaaact
     1681 ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa atcgttcgac
     1741 aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt ggtttagctt
     1801 atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc cgttttcagt
     1861 aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat ttacccctgt
     1921 taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg tttcctgaac
     1981 atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg cttctaaagt
     2041 attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg gttttgtctg
     2101 aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga aaaaaactgc
     2161 aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg cttgtgggat
     2221 aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa caatgatata
     2281 aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta aaagcgtcat
     2341 agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca tactttgtgc
     2401 caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc caatcacaat
     2461 aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt cccgaatggc
     2521 tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca gtcgaatccc
     2581 aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc aacgattagc
     2641 aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat cagaattaaa
     2701 acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca aaaaagtaat
     2761 gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca aagagattgg
     2821 ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga aataaactaa
     2881 cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat gttgagaaaa
     2941 aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta aaataaaacg
     3001 ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca tcagcaagac
     3061 atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta agctacgttt
     3121 caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat gaaaaaggaa
     3181 agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc aatgactaat
     3241 tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat aaatcaaggc
     3301 catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg tatatcgttt
     3361 cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag caactggaag
     3421 aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc agccaatata
     3481 tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat aacgacgccc
     3541 tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga taaatccagc
     3601 ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa tgattggaat
     3661 aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga tggaatcacc
     3721 tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa aataaatcgt
     3781 cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt ttcgagaggc
     3841 agcaatattc attattcatc aactccttag aggttctttg ttaactatac tttactagaa
     3901 aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg taagttcatc
     3961 ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt aataacttgt
     4021 tttttt
//
LOCUS       DAZKBH010000162         4021 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000162 DAZKBH010000000
VERSION     DAZKBH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.166"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..446
                     /locus_tag="ACG8OO_002696"
     CDS             <1..446
                     /locus_tag="ACG8OO_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046527.1"
                     /translation="GNLMIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKC
                     GNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVE
                     DAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(507..872)
                     /locus_tag="ACG8OO_002697"
     CDS             complement(507..872)
                     /locus_tag="ACG8OO_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFP9046528.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(948..1193)
                     /locus_tag="ACG8OO_002698"
     CDS             complement(948..1193)
                     /locus_tag="ACG8OO_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9046529.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1263..1517)
                     /locus_tag="ACG8OO_002699"
     CDS             complement(1263..1517)
                     /locus_tag="ACG8OO_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046530.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1681..2004)
                     /locus_tag="ACG8OO_002700"
     CDS             complement(1681..2004)
                     /locus_tag="ACG8OO_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046531.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2191..2264
                     /locus_tag="ACG8OO_002701"
     tRNA            2191..2264
                     /locus_tag="ACG8OO_002701"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2225..2227,aa:Ile,seq:cat)
     gene            complement(2649..3146)
                     /locus_tag="ACG8OO_002702"
     CDS             complement(2649..3146)
                     /locus_tag="ACG8OO_002702"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFP9046532.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3186..3995)
                     /locus_tag="ACG8OO_002703"
     CDS             complement(3186..3995)
                     /locus_tag="ACG8OO_002703"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9046533.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT         1343 a          715 c          712 g         1251 t
ORIGIN      
        1 gggggaacct tatgataaaa aaactatttt tactatcaat attaacgcta gcaatggcag
       61 gttgttctga ctctggctta gcaactgaac tgatcacaaa atcaactgaa gccacgacag
      121 aggagttaaa atgtggaaac gatcacagtg actacagagg cttaaatcga cgatgcatgg
      181 gaatattagg cagaagcaat tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa
      241 gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag
      301 acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg ttcatttatg tctagggaag
      361 ttggcgatga accaaaacta gagtacgctt ttagttttgc acgtgaggat gaagataacc
      421 ctaaaactta cgacatttta gcttgatcta agatggttaa agctttttaa tgaagtaaga
      481 ggagatagaa ggcttttcta gttatattag gtaagataaa ttcctttttg ctccaaattt
      541 gctaaacatt ctgctagata aacttcatcg tgttccggat aaatcggcac gttgcgctct
      601 acagtaggta aatcaaaaaa tgcttcgcat gatttgccac gatacatagg atttttccac
      661 tctggtgctg ggctgtaacc acgtttttcc atttcatcca tgacaagctg atgatattgg
      721 aataatctat aaggagaata gtgaaaaaca taatccacag tagcatgttt ctttccccag
      781 ccatttcctc ttaatgcaca gcattcccga tgttgtccta aaagctgttg acgaggaagc
      841 tttgaaatca atttttcatg ccataatcgc atagaaatgt ctcctttata tatagattgt
      901 taaccttaga cgttaaatga aaaaaccagc accaaggctg gaaaaaatca cttcagtttg
      961 cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata
     1021 tagattaatt caatgatgct tggaatttct tctccttctt cgtacttacg atatttgaat
     1081 aaaggaatac ttaacgcgct agaaacagct acttgagata aagatcggcc agttcttaag
     1141 aaattcagac gacaacgtaa agtatccagt aaaatagctt cttcgatact catttaatca
     1201 cctttcatta tagtagaata gttataatga ctatagaaag ctacacatgc aatcaatagt
     1261 actcaatgtt ttttatttaa acgtatcaaa taaagaatga cgatcaataa agacaaaacc
     1321 agtcctgata attcttctat aaaaacacta tcgggttcaa agtagttgta attaaaaact
     1381 ttccacataa agtagatagc cggaagagtc aaaatagcta aataaaaccg ttctttgttc
     1441 caattcataa gataaacaat acttgcgacc agtgcgaccg caggcaccca aaacaaaact
     1501 ctcaccaacg taatcatgta acggactcct caacgattca tataataaaa gataacatgt
     1561 tattgcatac atcacaaatg aaatgctagg aaaataaaag aagaatagat caatgaaaaa
     1621 tgaaaaagca gaacagtttt ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat
     1681 ctatcgagaa aaaatcaatg gattgatatc taagtaattc aatacatata aaaagaggac
     1741 gatcgcattc actaaaaagg aaagaatgat gatcccatag cgaatggaaa catatttaac
     1801 aaatactttt gtttgaatac gggcatatct ttttccttta tagctgagca tcatgacaag
     1861 aagcaaaagg gtaatcacac cttgaaaaat cattccaata aacagaattc ctgaggtttg
     1921 ataatttaat tggagaaaat aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa
     1981 tggcaaaata atcactccta acattgagaa taaataaaag aataacattt tttctaaaat
     2041 aagcaaataa aaattaaagt aacaaaatag ttatgacaca agtaaaagaa ataagaataa
     2101 caatattatt ttacttatat tttgaaactt gtgagagttt gtcattcgtg ctatagttga
     2161 aaagtaacaa aaataaattt tatttatgat gtacctttag ctcagttggt tagagcagac
     2221 ggctcataac cgtccggtcg taggttcgag tcctacaagg tacataagag gttgtgaaaa
     2281 agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttact
     2341 tggctgttct tattactgca gatgcaagct tttgaatacc gtttaaggaa gaggttgtga
     2401 aatcagcgtt ttgacctgag tattagctta acaaacggaa aaatagttcc tcatattttg
     2461 ccgtttgtta agctaatacc gagggtcagc tgattgaaca ccatttaaga aagaggtcgt
     2521 gagcctgccg tttcactctg tgtcataaaa aagccaatct actttgatag aaaaagcacg
     2581 taggactaaa acagttctgc gtgctttttt tattgtgtgt taaatatcgt aaatgaagat
     2641 agatattttt atgattcgac gatagagacg ttgaaatctt tcaacagctc taaagcggat
     2701 tctccgtgtt ttctactgga gactaacatt ggatcaacaa aaaagtctgc ttcaggaaaa
     2761 gcactctgca ggcctatcag attagagagt acacacaagt ttgtttcaat cccgactact
     2821 tcgatctctt tttgttcttc tttgctttca aataaagttt cctggatttc agctaaagtt
     2881 tcaggtggaa tcgtaaagta atacttttta atcactcgtt cattttcttg tggagaaaga
     2941 agagggatca attgcaaatc ttctcgttct tcttctttgt ccttcagttc aactggtata
     3001 tcccgtgtga agagaatata ttcattattt tggcgtgctt tagctaatct ttgagcaata
     3061 cgttctgcca attgatctga ctctggaata taaaattcgc tgtttggatc aagaatatga
     3121 ttttgcatat caataataac taacattttc tttcctccta cattcatatc gtcattttat
     3181 tctttttaat agaagttttt taaaatataa gcaacaccag cttcttcatt cgtcagtgtt
     3241 acttggtttg caatttcttt tacttgtcgc tgtgcattcc ccatcgcaat actcaacccg
     3301 ctgtaattca acatagcaat gtcgttttct tgatcgccaa aagccatgat gtcactcgat
     3361 tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag ctttagtgat acctgtaggc
     3421 gtgatttcta agaaatgcgg atcagagaat actgattgga taccatctgc ttgcagatct
     3481 ctttgtagct tctgtagttt ttcttcactc ttatctgtta atgctaattt aagatagcta
     3541 agagaaggat cttccaacat atttaaaatt tgatcaaaag aatggttctg taaattgaaa
     3601 tgttcataat aagggtcatc tttgactaga tttgattcat cgaatgtcag ctgatcatct
     3661 tgggtttctc ggctagcaat gatggatatt ttcgcttctt gagcatagaa gaaggctttt
     3721 ctcagtgtat cgctatctat ttttgaacgt ctgagaactt tcgccatttt tccatccttc
     3781 atttcagcaa tcagtgaacc attggaagaa atcaaatagc caccgattcc taattcggaa
     3841 aaaatatctt tagcagaaat gctgttcctt ccggtagcga cggttacgat tccgccattt
     3901 tcatcaaatt tccgaagtgc ttggatattt tcttctggta tctttccatt tcgatcaagc
     3961 aaggtaccgt ctaaatcaat cgctgctagt ttcattttat cttcctactt tctttttaca
     4021 t
//
LOCUS       DAZKBH010000163         3925 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000163 DAZKBH010000000
VERSION     DAZKBH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.167"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACG8OO_002704"
     CDS             complement(573..1130)
                     /locus_tag="ACG8OO_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046534.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACG8OO_002705"
     CDS             complement(1309..1920)
                     /locus_tag="ACG8OO_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046535.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACG8OO_002706"
     CDS             complement(2033..2290)
                     /locus_tag="ACG8OO_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046536.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACG8OO_002707"
     CDS             complement(2887..3165)
                     /locus_tag="ACG8OO_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046537.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACG8OO_002708"
     CDS             complement(3199..3450)
                     /locus_tag="ACG8OO_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046538.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DAZKBH010000164         3905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000164 DAZKBH010000000
VERSION     DAZKBH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.168"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..1114
                     /locus_tag="ACG8OO_002709"
     CDS             179..1114
                     /locus_tag="ACG8OO_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9046539.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            complement(1199..2209)
                     /gene="gap"
                     /locus_tag="ACG8OO_002710"
     CDS             complement(1199..2209)
                     /gene="gap"
                     /locus_tag="ACG8OO_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9046540.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            2497..3810
                     /gene="obgE"
                     /locus_tag="ACG8OO_002711"
     CDS             2497..3810
                     /gene="obgE"
                     /locus_tag="ACG8OO_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFP9046541.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
BASE COUNT         1223 a          694 c          878 g         1110 t
ORIGIN      
        1 tacactcctt tgttttcttt cgtcggaaat acaatttgag tgtaccataa atgccttttt
       61 atttttctaa cttaatttta caattcgcgg gttataaaaa tgaacattgc atagtagaga
      121 ttctttgatt atactaattc aaggttatat gaaaaaagag aaatgagatg gatgatttat
      181 gaaactttgg atgaaaatcc ttttaggcgt cgctttagct ctgattgttg cgttagtgtt
      241 tgcagggaat tatttttata attatgctgt tgtgccttct gagaaagact ttttagacga
      301 agatacacct ggtacaacac agacatccaa agaaacggat gcacataaat ggtttagttc
      361 aaaagaaaac cgaagcgatt ggaccatcac ttcaaaagac ggtctgaaat tatcagcgat
      421 ttatcttcct gctgaaacga aaagtgaaaa aacagctctt gtggctcatg gctatatggg
      481 aaacgctgaa acgatgacca attacgcaaa gatgtttcat gatatgggtt acaatgtcct
      541 tgttcctgat gcaagaggac atggaaaaag cgaaggggat tacattggat ttggatggcc
      601 agagcgtaaa gactatgtac agtggataaa caaagtgcta gaaaacaatg gaaaatcgca
      661 agaaatcgtt ctatacggtg tcagcatggg agcagcaaca gttatgatga ctagcggaga
      721 aaaactacca gataatgtga aagcaatcat tgaagattgc ggttattctt ccgttcatga
      781 tgagcttgct tatcagctag acgatatgtt tagtcttcca gcatttccgc ttatgcaagt
      841 aacgagtcta gtgacaaaag tccgtgctgg ctactttttc ggtgaagcaa atgcagtgga
      901 acaattgaag aaaaaccagc gcccaatgct ctttatacat ggagacgaag atacatttgt
      961 accatttgaa atgttagata aggtctatcg tgcaacgaag ggaccaaaag aaaagtatgt
     1021 tgtaaaagga gccgagcatg cggaagcata caagacagat cctgaaaaat atcagcaagt
     1081 agtccaacaa tttttaagcc aatatgttca ttgaacgagt ttaaaagtag gggatgaaaa
     1141 gaaagaagcc tttgactgga tccagtcaaa ggcttctttt atcgattgat ctatcaaatt
     1201 acaagttagc gaatttttct agtaagcgaa tcatttggca agtaaatcca tactcattgt
     1261 cataccatgc aacagtttta acaagttgga aatcaccagc agttgttact tctgtttgcg
     1321 ttgggtcaaa gatcgaacct tgagttgttc caatgacatc gctagaaacg atttgacggt
     1381 catcataccc gaatgaagga ttgtcaactg tgtgtttttt gattgcttcg tttacttgat
     1441 cagcagttac ttttgttttc aatactgaaa ccaattcagt taatgaacca tcaacgactg
     1501 gaacacgttg tgcgtgtcct tgtaattttc cttgtagctc agggataact aagccgattg
     1561 ctttcgcagc accagtagaa tgtgggatag tattgtcagc agctgaacga gcagcacgca
     1621 aattaccgcc ttttacaggt ccatctaaga gcatttgtgt ggaagtgtag gcatgaactg
     1681 ttgtcattgt accaacttcg atcccaaatt cattatttaa gaaataagcc ataggtgcta
     1741 aacagtttgt tgtacatgaa cctgctgaga tgattttgtc gttttcatct aatgtatcat
     1801 cattgacgtt atagacgatg gttttcatag caccagctgg ggcagaaata actactcgtt
     1861 tgacaccagc atctaaatgc gcttttgctt tttcttcaga tgtataaaaa ccagtacatt
     1921 ctagtacgat atctacaccg ttttcttttg cccaagggat tttgcttgcg tcgggttctg
     1981 cataaacacg agttgcttcg ccatccacta cgattgagtc ttctgttgca gtaactgtgc
     2041 caggataagt accatgagta gagtcaaatt gcaataattg tgctaacata gttggacttg
     2101 ttaagtcgtt gattgctact acttcaatat catctgagac ttctttgatg cggcggaaag
     2161 ccagacggcc aatacgccca aatccgttaa tacctacttt tactgtcata tcaaaagttc
     2221 ctcctttatt aggggtgctg tgaaacggca tcctcaatta atacaattaa tacactgtta
     2281 ttctgctacg caaagaatat tttgtcaaaa aattatactt ggtagcagaa aaaaggttgg
     2341 aaaccttacc aaatgataaa aaaagaagaa ggtaaacaat ctttttgctg aaatgataga
     2401 caaaatcaga tttcaccgat aaaataagta agacttttgt ttattgtgga aattcaggca
     2461 gtcaaataaa taaatgaata ataggaggac ttaattatgt ccatgttttt agatcaagta
     2521 acgatcgatg tgaaagctgg caagggcgga gacggaatgg ttgcatttcg tcgcgaaaaa
     2581 tacgttcctg acggcggacc ggctggcgga gacggtggtc gtggcggcga tgtcatcttg
     2641 atcgtcgatg aagggttacg tacattgatg gattttcggt tcaatcgcca tttcaaagcg
     2701 cagccaggtg aaaatgggat gagtaaaggg atgcacggtc gtggttcaga agatacctat
     2761 gttaaagtac cacaaggaac cactgtccga gatgcggaga ctggtgctct tttaggagat
     2821 ttaattgaaa atggtcaaac actagttgtg gcaaaaggtg gacgcggtgg acgagggaat
     2881 atccgttttg cctctcctcg gaatcctgca ccggaaattg ctgaaaacgg tgaaccagga
     2941 caagagcgaa aaatcgaact agaattgaaa gtattggcag acgttggact tgtaggtttt
     3001 ccatctgtcg ggaaatcgac tttactatcc gttatttcat ctgctcgtcc aaaaatcggc
     3061 gcttatcatt tcacgacgct cgtacctaat ctaggaatgg ttactacaag tgatggcaga
     3121 agttttgccg cagcagactt gccaggcttg attgaaggag cttcgcaagg tgttgggctg
     3181 ggtacacaat tccttcgtca tatcgaaaga acacgagtaa ttttacatgt gatcgatatg
     3241 agtgggatgg aaggacgaga tccttatgaa gactatcttg ccatcaacaa agaattatct
     3301 acttataact tacgcttgct agagcgtccg caaatcatcg tggcaaacaa aatggatatg
     3361 ccggatgcac cggaaaatct agtgaaattc aaagaacagc tgaacaagga aaaagaagat
     3421 gaatttgctg atgacatccc agtatttcca atttctggag tgactcgaca aggattggac
     3481 gcgttattga atgctacagc agatttgtta gaagtaacgc cagaattccc attgtacgaa
     3541 gaagaactgg aagaagaaac tgtacactat ggattcaatc cagaaggtcc tgagttccaa
     3601 atcgatcgtg attcagatgc aacgtggatc ttatctggtg agaagatcga aaaattattc
     3661 caaatgacga atttcgatca tgatgaaaca gtgatgcgtt ttgctcgtca attacgtggt
     3721 atgggtgtgg atgaagctct tcgggcacgc ggtgcgaaag atggcgacct tgtacgtatc
     3781 ggtgagtttg aatttgaatt tgtcgaatag gcaagcaaat aaaaacaaat accaaaaaat
     3841 atagattcag atttaaggga ggttgtgaca gaagtgatca gcttcaagaa cgccgtttat
     3901 tcgga
//
LOCUS       DAZKBH010000165         3876 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000165 DAZKBH010000000
VERSION     DAZKBH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.169"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2257
                     /locus_tag="ACG8OO_002712"
     CDS             158..2257
                     /locus_tag="ACG8OO_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFP9046542.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="ACG8OO_002713"
     CDS             2453..3316
                     /locus_tag="ACG8OO_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFP9046543.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="ACG8OO_002714"
     CDS             3421..>3876
                     /locus_tag="ACG8OO_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9046544.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DAZKBH010000166         3761 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000166 DAZKBH010000000
VERSION     DAZKBH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.170"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            446..592
                     /locus_tag="ACG8OO_002715"
                     /pseudo
     CDS             446..592
                     /locus_tag="ACG8OO_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(680..973)
                     /locus_tag="ACG8OO_002716"
     CDS             complement(680..973)
                     /locus_tag="ACG8OO_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFP9046545.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1010..1195
                     /locus_tag="ACG8OO_002717"
     CDS             1010..1195
                     /locus_tag="ACG8OO_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046546.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1376..2554)
                     /locus_tag="ACG8OO_002718"
     CDS             complement(1376..2554)
                     /locus_tag="ACG8OO_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9046547.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2658..2972)
                     /locus_tag="ACG8OO_002719"
     CDS             complement(2658..2972)
                     /locus_tag="ACG8OO_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9046548.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3079..3297
                     /locus_tag="ACG8OO_002720"
     CDS             3079..3297
                     /locus_tag="ACG8OO_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046549.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(3217..3687)
                     /locus_tag="ACG8OO_002721"
     CDS             complement(3217..3687)
                     /locus_tag="ACG8OO_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFP9046550.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
BASE COUNT         1201 a          721 c          553 g         1286 t
ORIGIN      
        1 aaaatactct ttttttacga ttattgaaaa aaaaagataa aacttgatta attgaagtat
       61 tgcttaaatt tggaagctat gagggaagaa taaaagagtt aggacgggat tatctactat
      121 ttttctttgt tttaaatatt tgttatcttt gaacataaaa gagaaatgtg tcgactgcag
      181 tgttgtgtcc ctcatcacga ataaacggtg ttcaattagc tgatcttgta ttcacttaaa
      241 aaatggtaaa gtacgagttg ctattcccca ttttcaattt tctaacgctc aggacaaaat
      301 actgttttca caacctcatt ggaataaaag catatatatt gtttcaagaa cgaaatcttg
      361 ttatcttttt aaagggcatc tgtccaaaat ctaattttag gaggtacgtt ttggtattta
      421 ttcgtacaaa taaaagagaa ttatctttat tcatgtaacg catccaattg attttttcat
      481 gtacgctcct ggaacgattt tactgacgat tgcttattta actgcgaatc gctcattagc
      541 ttttatcata gctatccata ttttgaataa cgttcttggc tttgtattat agatacagat
      601 gacaaaaaaa gttcaagtat gcggtgtaga accaatatcg catacttgaa cttttttcaa
      661 tgagtataaa ttaaaacttc taattttcaa ttcccatcat ctgatatgcc cgaccgtctg
      721 aacttctttt gaaaaagcca aatgtgatta gataacgtct ttgttcgaca taatcgccac
      781 tagaaaatac gttcagcagt atcgaattaa cctctgtttc tgtataagtc actccatggt
      841 caaagcgttt caagacttct ttcaatagga tgtatttctt ttttgttgaa cttggtactt
      901 gttttacttt tccatcattc atgaatgtgt ctaaaacttt catcttttct tgctccgtat
      961 aagtataaac catcttcttt ctccttttga ttctaagaat actccattta tgttagaata
     1021 ttctggattt tttattaaag cggttactct taagaaagat attatcatag tgctcttttt
     1081 tttactacta cttataggta gatttattct aatccattat tctcccctac caataattaa
     1141 agaaaaaact ggatcagggt gttcaaatta catgctaaga aaaaagaacc cttgaatacc
     1201 aagggttctt tctcagtctg ataacaatga ttaacgacgg atttctttga tacttgtgaa
     1261 aagtcgttaa agtattgata taaaaggatt tttattacct ctgactacat attgactaat
     1321 ttgttaaaaa atcgtttttc aagaatcctg taataccagt gctttcaccg tttttttaac
     1381 tctctaaaga actaaaagca atcagaggtt ttaccttttc atttgacgtt aagtggcgat
     1441 aaattttcga agttgtttgg tcagtatggc ctaagcgatc tttcaagact tcccagctac
     1501 agtgttccgc tactaagctt gccattgtat gacgagctaa atgagtatga aatggcttat
     1561 taattcctga gcgattcacg caccgtttga cgtgatcatt tacatactct cgacgataag
     1621 gaacaccacg tttactagaa tcaagataca caaacaaata tgtttcttct ataataaacc
     1681 tgtaatgttg cctcatctca cccaaagcct taatcaactg ttgtagcttt tccataacta
     1741 gaggagttac tagaactcgt ctagcagaac taactgtctt agtagtttca attcgaaaat
     1801 ccttactctt acctttaact gccgtttgct tatagaacag aatttcattt cttttgaaat
     1861 ctatatctcc aggctgtaga gcaagcgcct cgctgactcg gcagccagtg taaaatagaa
     1921 catggaaaag tacagacatc ggtaattcct catgaacaat gccataattc aaaaaagttc
     1981 tcaattcatc gacagtaaga aacttgtcct caatattatt tatggcttct tttaaatcag
     2041 ttaccgttaa ctgatatttt ggcattctca cagacttcat tggattttca cttatcaaat
     2101 tctttctttg acaatattta aaaatcatat ttagaacttg aataagtgat tgtaaactat
     2161 ttttggcata gccaacattt gttccattaa cgccacattc ctttaaggta aaaagcaatt
     2221 cttgaaggca ctctggtgtc aaatcagaca actttttatc accaatccat cgaacaataa
     2281 tttttaattg ttcctctcgc ttatcatagg ttatttcctt ttgttcattt aacgaacggt
     2341 aatattggaa ccattgctca cctacaacac taaacttgtc attgcttgca acaatttttt
     2401 tcttaagcct ttgttgtttc tccatttcca gataagcgag ttgcgcctca cgttttgaat
     2461 taaagccacg tcttgttgtt cgatatgctt gaaagtacca tgtgatccta ccagtagaac
     2521 tgatttgctt cttgaatcga tctgtttttt ccatttattg attcctccta ttttgaagga
     2581 acccaacaag cagaaaccct ttggaaatct ttctatctat gtcatcattg taccgcatct
     2641 tagaaagaaa atgtaactta taaagatcga agtagtcgca atttttctcg atagtcattc
     2701 gaatcactct ttctcatcca ttctaaaaca tcatcacgaa aatagtgtcc tcgaatcccg
     2761 taccaacagg gaggaaactc accaagtttt actaaacggt tcagtgtact atcacttatc
     2821 tgaaatagtc gctttaaatc agtatttgtt aataccagag gtaaatgact gactgcaagc
     2881 aatttttccg tgtaatcttc cagttttttt tgtaaaattg aatagaactc tatttccaga
     2941 gaattaacat ttgttttaac ttccaactcc atcacccttc tctctcaaaa tcaaaacgac
     3001 caccacggta ccccttaaaa ttgtaaaaaa acactccagt actccatcaa aacggactag
     3061 aaaagaggaa tttttaatat gaaacaagga attgcagaca taaaaataat taaagaaatt
     3121 cttgaaaaat ctacggctaa tgcaatagca ttcggaacag gaatcaactt gagtacagta
     3181 aaaaaactta aaagtggaga gagagctgaa gaaaaattaa atcttgccga tgctattaaa
     3241 atcacagaat ttggcatgaa aaatatgcca accaaaatcg aaatatggaa atcctagctc
     3301 ttccaaagcc atttgtcgtt ggtaccaaac actgccgaga cttttcccaa taatatcgcc
     3361 aatttcacga tcattaagac cgatgaagaa ggacataaaa atgatttctt gtctttgact
     3421 aggtaatgct gcaatcgcat ttgccaactc ctcattggaa ataaagattt tcataccaag
     3481 gacaataaaa gtaccactac tattcaaatt tggttccaaa taatagaact ctgtacctaa
     3541 ccctttttgt tccaatattt ccattgatat ttgccgttgg cgaaatctag cgtacttctt
     3601 ttgaatattt aacgcctcat tgcagactac tttcttacaa aaactgtcaa acgtatgttg
     3661 aatgtgggtt tcctctgagt cgcccataat attctccccc tcaaaaaatt cattcaactc
     3721 gttttacttc tatccctttc cattattact acaatctagg a
//
LOCUS       DAZKBH010000167         3713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000167 DAZKBH010000000
VERSION     DAZKBH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.171"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG8OO_002722"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG8OO_002722"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9046551.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACG8OO_002723"
     CDS             complement(2260..2511)
                     /locus_tag="ACG8OO_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFP9046552.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACG8OO_002724"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACG8OO_002724"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9046553.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACG8OO_002725"
     CDS             3461..3679
                     /locus_tag="ACG8OO_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046554.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1026 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca aga
//
LOCUS       DAZKBH010000168         3617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000168 DAZKBH010000000
VERSION     DAZKBH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.173"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(30..323)
                     /gene="rpmA"
                     /locus_tag="ACG8OO_002726"
     CDS             complement(30..323)
                     /gene="rpmA"
                     /locus_tag="ACG8OO_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9046555.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(336..680)
                     /locus_tag="ACG8OO_002727"
     CDS             complement(336..680)
                     /locus_tag="ACG8OO_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFP9046556.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(707..1015)
                     /gene="rplU"
                     /locus_tag="ACG8OO_002728"
     CDS             complement(707..1015)
                     /gene="rplU"
                     /locus_tag="ACG8OO_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9046557.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(1035..1111)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1297..1938
                     /locus_tag="ACG8OO_002729"
     CDS             1297..1938
                     /locus_tag="ACG8OO_002729"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9046558.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(2000..>3617)
                     /locus_tag="ACG8OO_002730"
     CDS             complement(2000..>3617)
                     /locus_tag="ACG8OO_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFP9046559.1"
                     /translation="VSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGVSSGAD
                     AAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATFLAGLF
                     LLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEKLSSYQ
                     TIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPVATVGK
                     IPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKPLDSNQ
                     ELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSMLVFAPV
                     MSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDLSIAIV
                     IGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMSSEKLK
                     NKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEVEKQLS
                     HLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         1101 a          808 c          650 g         1058 t
ORIGIN      
        1 gtcacaacct ctttaatcat aagattcaat taagcttctt gagctactgg ataaacagac
       61 acttgttttt tgttacggcc tttacgttcg aaacgtacta cgccatcaac ttttgcaaat
      121 aaagtgtcgt ctccaccgat acccacgttt acacctggat agatttttgt accgcgttga
      181 cggtaaagaa ttgatccacc tgtaactgtt tgtccatcag cacgtttagc acctagacgt
      241 tttgattctg agtcacggcc gttagatgta gaaccgccac cttttttgtg ggcaaataat
      301 tgtagattca tagataacaa catggtctgc acctcctatt tatcattaat tgttttaatt
      361 tggatatatt ctgagttttc agcttctatc gattgtaatc ctaataaaag attttctaat
      421 agaatctcag caatattgtt ttgttcttgt gtcattcctg aagtgacttc ggtataaaga
      481 taaccgcctt cttcttcatt catttcaaca atcggctcaa aaccggctaa tgaggcaatc
      541 ccgttgacag tgctgatcgc taacgcagaa acacccgcac aaacgatatc acttccataa
      601 ggaccagcat cagcatgccc ggtcaaagtg aatgaaacga tctgacctgc gtcatttcgc
      661 ttaaatgttc ctttgatcat atataggcct tctttctcaa acgctattaa gcgttgatcg
      721 cgtcgataac cacttttgta tatggttgac gatgaccttg tttacggtgt gaatgttttt
      781 taggtttgta tttgaaagta acgactttct tttgtttgcc gtgtttttca acagtacctt
      841 caacagttgc acctgcaaca gttggagcgc ctacttttgt agattctccg cctactaaga
      901 taacttcgtc aaaaacaact ttttctcctg cttcaacgtc aagtttttca acatagattg
      961 cttgtcccac ttcaactttt acttgtttac caccagtttt aataattgcg tacatgctat
     1021 aaagcacctc cttctgatat acttagactc gccatccgaa gtgacagcat atgctgtgct
     1081 tattacttgt tctgagcggt tgtagctgtg gagtccacaa ttacaacagt actagcttac
     1141 cagaacagca accgttagtc aactaaattt aaaatcaaaa agtaaaacaa aaattcaagc
     1201 aatacttctg cttttctatc ctcacagttc taccaaataa aatagtcgca ttgtgtttct
     1261 tttattctta taataaaaga aaaaggagga gcataaatgg aatacccaaa agaactgata
     1321 caagaagctt ctttaagaat gaacggccgt cctgtagaag gatttatcgc tgggcaagga
     1381 cctcttcatc ctaaactgat gctcgtggga gaagccccag ggaaaacaga gattgatacg
     1441 catgttcctt ttagcggtca agctggaaaa gaattgatgc aggcactttc ttcaactggc
     1501 ttaacacgag aagaggtgta tatcaccagt gctgtaagga gccgcccgta tcgcgtgact
     1561 catcggatca ataaacggac acaacagtca gaaactgttt atccaaatcg gacacctact
     1621 cgttccgaag tatttgcgca tgcaccaata ttggattacg aactgatgaa ggtccagcca
     1681 aaattaattg cgacacttgg aaacattggt cttcaacgtt tacttggcaa agaatatacg
     1741 gttaccaaaa atcatggtca actctataca gggcctgtct tacaattaac agaacaaaag
     1801 gatgcctacg aatggtcaga aaaaaactat cgaattcttc cactttttca tccagcagct
     1861 attttctaca atcgctccct tgtacctgtt atccatcaag actggcaagt actagcttca
     1921 cttttacagc aatattaaaa agaacagaca agaccataaa atcttcagtt tacagtcttg
     1981 tctgttcttt ttcattgttt taatggctaa ttgcagtata tttcgcatgt aaagctgtca
     2041 acgcatgagc aactgtttga tgttgatgcg tgaatgtatg ttcccacaag tgagaaagtt
     2101 gtttttctac ttctggctgc aaggaagtat agataatcgt ttgtcctctt tgctctgctt
     2161 tttcttgaaa ttcatcaaaa actgcacgtg ccgtcaaatc aatacttggt acgcctcgca
     2221 tggagaaaat gacaccatcg tgatgattta gttcttccaa tttatttttg agtttttctg
     2281 aagacataaa aaacagtgga ccactaatat acactactgt ccagttattc agcttttccg
     2341 tttctggcag ccccattctt tgccaatcga tcgtttctaa tgaaatcgtg atagcagcgc
     2401 ttttgacaac aaaaaataca cagccgccga tcaccccgat cacgatagca atacttaaat
     2461 caaagataac tgtacaaatc atcgtaagga taaacaatag aactgctgac caatatttat
     2521 tagtaaatag ctccttgatc gtttcccatt cattcatacg ccaagccgtc acaatcaaaa
     2581 cacctgcaag tgctggcatt ggaatctgtg acatcactgg cgcaaaaaca agcatggaga
     2641 gcagtaagac aactgcatgt atcataccag ctaatctagt ttgtgcacca gatttgatgg
     2701 ccacgcttgt acgggcgatg gccgcagtag caggaatccc gccaaagaaa ggaagcaaca
     2761 gatttccaat tccttgcgcc attaattctt gattgctatc taagggctta tttgccattc
     2821 ggccagccga agcaccacat agcaagcttt caatcattcc taataaagca atgctaatag
     2881 ctggaactgc cacttgttgc aaggcagaaa agctaaaatc tcccatattc aaacgattac
     2941 tactgatcaa tgtttgtgga atcttaccca cagttgctac tggtaaatgg aacaatagca
     3001 taactgctgt tgccaaaatg atagccagca gcgaactagg cattcgtttc gcccattttt
     3061 ttggatagat aaacatccct aaaatgacta gccctcccat gatcaaagag gctgtgttta
     3121 tcggaaatcc tatcgtttga taactactta gtttttcaat aacgtttgta ccttccgagt
     3181 gaacaccaaa taaattatcg atctgaccta atgcaataat gatagcaata cctgaagtaa
     3241 accccgtaat tacgggtgct gggataaaag aagtcagtgt tcctaatcgg aaaagtcctg
     3301 ctgcaagtaa aaatagaccc gctaaaaaag tagcaagcaa tacaccttgc attccctttg
     3361 ttgccgcaat agacatcaag atagctgcca tcgcacctgt cggacctgat atttgataaa
     3421 aaccgcctga taaaccgcca ataacgatac cggcaatgat agccgtaatc aaacctgcgg
     3481 ccgcatcagc tccgcttgat accccaaaag ccaaggctaa aggcaatgct acagcagcta
     3541 ctgtgatccc agccaaaaga tctttctgaa atcttgaaag atcataacct gaaaattctt
     3601 tttttagtga ggaaaca
//
LOCUS       DAZKBH010000169         3557 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000169 DAZKBH010000000
VERSION     DAZKBH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.174"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..989
                     /gene="vanS"
                     /locus_tag="ACG8OO_002731"
     CDS             <1..989
                     /gene="vanS"
                     /locus_tag="ACG8OO_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFP9046560.1"
                     /translation="YDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFA
                     KYFDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDV
                     VMYLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEIT
                     RYNLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARV
                     FNNILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNAR
                     SSDTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            1204..2172
                     /locus_tag="ACG8OO_002732"
     CDS             1204..2172
                     /locus_tag="ACG8OO_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9046561.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            2165..3196
                     /gene="vanA"
                     /locus_tag="ACG8OO_002733"
     CDS             2165..3196
                     /gene="vanA"
                     /locus_tag="ACG8OO_002733"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9046562.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            3202..>3557
                     /locus_tag="ACG8OO_002734"
     CDS             3202..>3557
                     /locus_tag="ACG8OO_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFP9046563.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT         1073 a          690 c          855 g          939 t
ORIGIN      
        1 aatatgactt aaatcacctg gacgcgatga aattatatca atattccata cggaacaata
       61 tagatatctt tatttatgtg gcgattgtca ttagtattct tattctatgt cgcgtcatgc
      121 tttcaaaatt cgcaaaatac tttgacgaga taaataccgg cattgatgta cttattcaga
      181 acgaagataa acaaattgag ctttctgcgg aaatggatgt tatggaacaa aagctcaaca
      241 cattaaaacg gactctggaa aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa
      301 atgacgttgt tatgtacttg gcgcacgata ttaaaacgcc ccttacatcc attatcggtt
      361 atttgagcct gcttgacgag gctccagaca tgccggtaga tcaaaaggca aagtatgtgc
      421 atatcacgtt ggacaaagcg tatcgactcg aacagctaat cgacgagttt tttgagatta
      481 cacggtataa cctacaaacg ataacgctaa caaaaacgca catagaccta tactatatgc
      541 tggtgcagat gaccgatgaa ttttatcctc agctttccgc acatggaaaa caggcggtta
      601 ttcacgcccc cgaggatctg accgtgtccg gcgaccctga taaactcgcg agagtcttta
      661 acaacatttt gaaaaacgcc gctgcataca gtgaggataa cagcatcatt gacattaccg
      721 cgggcctctc cggggatgtg gtgtcaatcg aattcaagaa cactggaagc atcccaaaag
      781 ataagctagc tgccatattt gaaaagttct ataggctgga caatgctcgt tcttccgata
      841 cgggtggcgc gggacttgga ttggcgattg caaaagaaat tattgttcag catggagggc
      901 agatttacgc ggaaagcaat gataactata cgacgtttag ggtagagctt ccagcgatgc
      961 cagacttggt tgataaaagg aggtcctaag agatgtatat aattttttag gaaaatctca
     1021 aggttatctt tactttttct taggaaatta acaatttaat attaagaaac ggctcgttct
     1081 tacacggtag acttaatacc gtaagaacga gccgttttcg ttcttcagag aaagatttga
     1141 caagattacc attggcatcc ccgttttatt tggtgccttt cacagaaagg gttggtctta
     1201 attatgaata acatcggcat tactgtttat ggatgtgagc aggatgaggc agatgcattc
     1261 catgctcttt cgcctcgctt tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa
     1321 tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg tgggacataa atcagagatt
     1381 tccgcctcta ttcttcttgc gctgaagaga gccggtgtga aatatatttc tacccgaagc
     1441 atcggctgca atcatataga tacaactgct gctaagagaa tgggcatcac tgtcgacaat
     1501 gtggcgtact cgccggatag cgttgccgat tatactatga tgctaattct tatggcagta
     1561 cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac
     1621 cgtggcaagg tactcagcga catgacagtt ggtgtggtgg gaacgggcca gataggcaaa
     1681 gcggttattg agcggctgcg aggatttgga tgtaaagtgt tggcttatag tcgcagccga
     1741 agtatagagg taaactatgt accgtttgat gagttgctgc aaaatagcga tatcgttacg
     1801 cttcatgtgc cgctcaatac ggatacgcac tatattatca gccacgaaca aatacagaga
     1861 atgaagcaag gagcatttct tatcaatact gggcgcggtc cacttgtaga tacctatgag
     1921 ttggttaaag cattagaaaa cgggaaactg ggcggtgccg cattggatgt attggaagga
     1981 gaggaagagt ttttctactc tgattgcacc caaaaaccaa ttgataatca atttttactt
     2041 aaacttcaaa gaatgcctaa cgtgataatc acaccgcata cggcctatta taccgagcaa
     2101 gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt tggattttga aaggagacag
     2161 gagcatgaat agaataaaag ttgcaatact gtttgggggt tgctcagagg agcatgacgt
     2221 atcggtaaaa tctgcaatag agatagccgc taacattaat aaagaaaaat acgagccgtt
     2281 atacattgga attacgaaat ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg
     2341 ggaaaacgac aattgctatt cagctgtact ctcgccggat aaaaaaatgc acggattact
     2401 tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat gtagcatttt cagctttgca
     2461 tggcaagtca ggtgaagatg gatccataca aggtctgttt gaattgtccg gtatcccttt
     2521 tgtaggctgc gatattcaaa gctcagcaat ttgtatggac aaatcgttga catacatcgt
     2581 tgcgaaaaat gctgggatag ctactcccgc cttttgggtt attaataaag atgataggcc
     2641 ggtggcagct acgtttacct atcctgtttt tgttaagccg gcgcgttcag gctcatcctt
     2701 cggtgtgaaa aaagtcaata gcgcggacga attggactac gcaattgaat cggcaagaca
     2761 atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt
     2821 attgggaaac agtgccgcgt tagttgttgg cgaggtggac caaatcaggc tgcagtacgg
     2881 aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac
     2941 cgttcccgca gacctttcag cagaggagcg aggacggata caggaaacgg caaaaaaaat
     3001 atataaagcg ctcggctgta gaggtctagc ccgtgtggat atgtttttac aagataacgg
     3061 ccgcattgta ctgaacgaag tcaatactct gcccggtttc acgtcataca gtcgttatcc
     3121 ccgtatgatg gccgctgcag gtattgcact tcccgaactg attgaccgct tgatcgtatt
     3181 agcgttaaag gggtgataag catggaaata ggatttactt ttttagatga aatagtacac
     3241 ggtgttcgtt gggacgctaa atatgccact tgggataatt tcaccggaaa accggttgac
     3301 ggttatgaag taaatcgcat tgtagggaca tacgagttgg ctgaatcgct tttgaaggca
     3361 aaagaactgg ctgctaccca agggtacgga ttgcttctat gggacggtta ccgtcctaag
     3421 cgtgctgtaa actgttttat gcaatgggct gcacagccgg aaaataacct gacaaaggaa
     3481 agttattatc ccaatattga ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa
     3541 tcaagccata gccgcgg
//
LOCUS       DAZKBH010000170         3505 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000170 DAZKBH010000000
VERSION     DAZKBH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.175"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="ACG8OO_002735"
     CDS             complement(<1..1097)
                     /locus_tag="ACG8OO_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9046564.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="ACG8OO_002736"
     CDS             complement(1227..2603)
                     /locus_tag="ACG8OO_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9046565.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="ACG8OO_002737"
     CDS             complement(2603..3259)
                     /locus_tag="ACG8OO_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9046566.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..>3505)
                     /locus_tag="ACG8OO_002738"
     CDS             complement(3269..>3505)
                     /locus_tag="ACG8OO_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9046567.1"
                     /translation="SSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLG
                     QSYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
BASE COUNT         1086 a          649 c          466 g         1304 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaaga
//
LOCUS       DAZKBH010000171         3347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000171 DAZKBH010000000
VERSION     DAZKBH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.176"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(564..1178)
                     /locus_tag="ACG8OO_002739"
     CDS             complement(564..1178)
                     /locus_tag="ACG8OO_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9046568.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1784..2161)
                     /locus_tag="ACG8OO_002740"
     CDS             complement(1784..2161)
                     /locus_tag="ACG8OO_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046569.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2461..3342)
                     /gene="galU"
                     /locus_tag="ACG8OO_002741"
     CDS             complement(2461..3342)
                     /gene="galU"
                     /locus_tag="ACG8OO_002741"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9046570.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1024 a          677 c          507 g         1139 t
ORIGIN      
        1 ttttttattt ttagattaga gaaaatctcg tcctgttttt ttcattttac aatcattgaa
       61 aaattgaatt tcattgcaaa aaaaataata aacacttata aatatccatt atctatcctt
      121 caatttttgt gtattttatt tctattagaa aaaggtatcc tgttggacaa ttttgttata
      181 tcaggtaaac ttcattgcaa taatgataac aaatgtacta taattacttt gtaaagagtt
      241 ttggggaaga ctctttcatc aacgtgtatt aagagacaag aaagaatcca tatggattct
      301 ttcttgtctc tttttccact actttttatt tttctgacac tacatattct tataactcat
      361 ttctcataac tgtgaaacaa cttataaaaa taacaaactt taaacaaaac aagcttaaaa
      421 tcaaagatga tccatcttaa aataactaaa acaaaaaaag tagtcgttgt taatcctcat
      481 taataatcgt tttctttttt tcaaacgctc catttttatg tggaacatta tttttttaag
      541 gaactttgtc aacatgtgga taactatttt ccacttgtgt cctggactag ctgttttgct
      601 gtaaaataca aaacttgctg cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc
      661 gctggatcta gatttggcaa agtcaagact gcatcatgta aattggcgtg gttgtccaaa
      721 accaaatcag caaattcaaa taatcgcaaa ccactgctat gttgcgattt gatacttcgt
      781 gacaaatcaa atccagtaac aatgattaat ggatgaccat tttctttgat ccactgagct
      841 agttcgatga ttgccgggtt tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat
      901 ttgacagaaa gctgatcaat aattgcctgt cctgcacctt ctagcgtttc atagacacct
      961 ccggctggat tttcaatcaa tatacttggt acaagatccg gggtttgatc ccaaaattca
     1021 aatgctacac cacttagtgt gcggctagca tatatctgca atctaccacc ttgattaatt
     1081 tgtttcgcga tccaagtgcc agcttgaagc atcagcggct cttctatcct taatttctct
     1141 tccaattggt gataatagtc aaaataacat aaactcattt atctctcccg cctagctata
     1201 cttgacatga atagaaacag ctgcctaatc taaggaagta cataaaaaaa cagtagttag
     1261 acagtaacac atgtggtctt tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc
     1321 caataccttg acatagcaaa taaaacaacg tgcagaccat ggaaaatatc tacatagggg
     1381 tagaattttc accctagatt aggtacaata aattaggtcg atgcactgca ttgactatgg
     1441 caaaatgatc ggtcagggtg tatgtgtgtg actggtcatt ccctgattct tagcgccaac
     1501 taagggtcag ggagcctttt tttattcagc tgtttctatc tgaatttatt gtaagtaatc
     1561 tcttatccaa gtgcaagcaa gaaaatacaa aaaaacattg gtatgattta tatatatata
     1621 aataacataa aaaaaggaat ggcttatccc gatacaacaa gcattccttt aaaaaagatt
     1681 cttttatata agtgaaaaaa gcttttttcc accaattaaa taaatctctc atctcaatag
     1741 tttatccatt attttatctt tttttgcgta tacctactaa atattaccgt cttcttctcg
     1801 ttcttctact ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt ctaccttttc
     1861 tgcctatccg agactttctt ttcatatatg ggatataaat gattagatac agtacagcaa
     1921 aaaataaagc aaatacgcaa gcttcccaac tacctaacgc caattcaacc atcaaggtag
     1981 caaccccaac ggatgctgca taccagataa aaatcagaaa cgtttctttt tttctgacca
     2041 tgcgaaacat gataaataga ctaataagca atagtggcaa cacaaatatc caattgcttc
     2101 cttccatcag ccacttgagt atatcaagca gcgactgtgc aacagcgact aattttccca
     2161 tagtgaacac acaccttttt ttataattaa aaaattcgcc tttttattct actatgaatc
     2221 ttctggcttg gctttcattt ctcttatgta atcattttct tttatacaac ttaaaaaaaa
     2281 attaaaccat tagcataata ttttctgaat ctattcacga aagtcttttt ctatctttcg
     2341 ttttccacga ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg
     2401 cacaagctgc cgctttaata acaaatagcc tttcattctc agcaggccac ttatttccag
     2461 ttaactttct gttcttagct ttttactcaa ctggataata tatgcactta atgaagtccc
     2521 aatttccgaa tgttgaagtc catattcaat atttgtcttc atatatccta acttatcgcc
     2581 tacgtcgtat ctttttcctt taaactctct cgcatagacc gcctctgtct ggttaagagt
     2641 ctgaagtgca tccgttagct gtatctcgtt tcctgctcct ggttcttgag tttctaatat
     2701 ctcaaatatt ttaggagtca gtagataacg tccgataatg gctaaattac ttggtgcttt
     2761 tcccatttca ggtttttcta caaacccttt tactctgcaa cgaccctttt cgctatctgt
     2821 tttaggatca ataattccgt atttagatgt ttcattttct ggtacacgca tgacggcaac
     2881 agcggaagct tgtgtttctt cataatccat catcaattgt tgagtcaatg ggatatcatc
     2941 tttcatcaaa tcatccccca gcatgacgac aaacggttca tttcctataa atgcttttgc
     3001 ttgaagcacg gcatccccca gtcctcttgg atgagattga cggatgaagt gaagattgac
     3061 ttgagtcgtt tcttcaacta aagcgagtaa ttctgttttt tccttttccc gcaaattgtt
     3121 ttccaattcg atatttgcat caaagtgatc ttcaatgggt cttttttctt ttcccgttac
     3181 gatcaagatg tcttcgattc ccgaatccaa tgcttcttcc acaataaact ggatcgttgg
     3241 tttatccacg attggaagca tttcttttgc catggctttt gttgccggca aaaaacgtgt
     3301 tcctagccca gcggcaggaa tgactgcttt tttgaccttc acttgct
//
LOCUS       DAZKBH010000172         3293 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000172 DAZKBH010000000
VERSION     DAZKBH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.177"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG8OO_002742"
     CDS             278..787
                     /locus_tag="ACG8OO_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9046571.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG8OO_002743"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG8OO_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9046572.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..>3293
                     /locus_tag="ACG8OO_002744"
     CDS             2322..>3293
                     /locus_tag="ACG8OO_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9046573.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKN"
BASE COUNT         1175 a          527 c          651 g          940 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aat
//
LOCUS       DAZKBH010000173         3221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000173 DAZKBH010000000
VERSION     DAZKBH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.178"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8OO_002745"
     CDS             301..585
                     /locus_tag="ACG8OO_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046574.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8OO_002746"
     CDS             588..1004
                     /locus_tag="ACG8OO_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046575.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8OO_002747"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8OO_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9046576.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACG8OO_002748"
     CDS             2544..2630
                     /locus_tag="ACG8OO_002748"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9046577.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACG8OO_002749"
     CDS             complement(2932..>3221)
                     /locus_tag="ACG8OO_002749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046578.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAZKBH010000174         3128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000174 DAZKBH010000000
VERSION     DAZKBH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.179"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1347
                     /locus_tag="ACG8OO_002750"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ACG8OO_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ACG8OO_002751"
     CDS             1360..1992
                     /locus_tag="ACG8OO_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9046579.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2200..2424
                     /locus_tag="ACG8OO_002752"
     CDS             2200..2424
                     /locus_tag="ACG8OO_002752"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP9046580.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2526..2759
                     /gene="relB"
                     /locus_tag="ACG8OO_002753"
     CDS             2526..2759
                     /gene="relB"
                     /locus_tag="ACG8OO_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFP9046581.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ACG8OO_002754"
     CDS             2749..3018
                     /locus_tag="ACG8OO_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9046582.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DAZKBH010000175         3121 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000175 DAZKBH010000000
VERSION     DAZKBH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.180"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..535)
                     /locus_tag="ACG8OO_002755"
     CDS             complement(167..535)
                     /locus_tag="ACG8OO_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFP9046583.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            685..1167
                     /locus_tag="ACG8OO_002756"
     CDS             685..1167
                     /locus_tag="ACG8OO_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFP9046584.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1204..2214
                     /locus_tag="ACG8OO_002757"
     CDS             1204..2214
                     /locus_tag="ACG8OO_002757"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFP9046585.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2329..3042)
                     /locus_tag="ACG8OO_002758"
     CDS             complement(2329..3042)
                     /locus_tag="ACG8OO_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046586.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         1051 a          502 c          618 g          950 t
ORIGIN      
        1 tcaatcagtg atttttttca gcacaagaaa gactccttat aaaaatagca gatactttgt
       61 caattaagtg tttaaccagg gatgataaaa aggataggat tgaacaaaac ctgtataagg
      121 tgatgaatag tttcctcgcc agtaaaaggt ttattcgtat aaagctttat cgccaaaatt
      181 tatcatgacc ttttttatct tcaatattaa atgaaatcaa ttgttcaatc aattcccagt
      241 tgattacact tttccaagga actttgatga cgttatccgt atatacgtag cctgcttctt
      301 ggatattttt ttcaagggca cgaatcgctg ctttttccgg tgcgatagaa aaatgatttt
      361 tagcaaaact aaatgcaata ataaaggtac catggtcagt aaacataggt tggttccatt
      421 ttacagttgt gtctagctgg ggatacgttt ctgttatctt agcaaaaagg tctttgatct
      481 tttgttgatg ttctttttct ttgatttgat tgatgatcgg ctgaaatttt tccattctaa
      541 aacgcctcca ttaagtggtt tgatttattt taccagaaaa acaatagtta tgtataaaaa
      601 acgaacaaaa cacaagtaat gttgtaaccg ctatatttat atgataagat gtctcaaata
      661 ataaaataga aaagagacat aaaaatgatt gacttggcaa agaaattctt acacgaaaat
      721 atatattttg agttgtatca tcatcgagcc atttatacaa atgaagatgc tttggtaata
      781 aagaaagaac aaggatttac aggtacagag accaaaagct tattcttaaa agataaaaaa
      841 gataataact acgtatatct gacttttact acgaaacaaa cggatttcaa agcattaggg
      901 aagatcgtcg gtaaaaggct ttctattgtt tcatcagaac gcatggaaga acaaacggga
      961 caaaagcagg gcgctgtttc tccgtttggt tatgaaaaac agattccgat tattattgat
     1021 gaagagctct tatcaaatga aaaacttgtt tttgcaccag gtagaccaga tcaaacgatg
     1081 gtcatccaaa cggttgactt aaacaaaatc atccagctac ttgagatgcc gatatttttg
     1141 atgccggcaa acgaaaaaaa caattaacca agtaaaatag tgcatacttg gaggtgaagc
     1201 aacatgataa gttatccatt attagaaaga aatgcaacga ttggggttac tgcaccatcc
     1261 tcaggcgtat cagaggaaca ccattcgtta ctcgaatcag ctttaaagag aatggaagaa
     1321 aaatctttcc aagtagttac tggaaagacg ctttggacgc aagataagga aaaatcaggt
     1381 ccagcaagaa aacgagcaga tgaattcaat caaatgatgc aagatgaaaa tatccaaatg
     1441 atctttccga cttggggagg agaattattg attgagattc tcgattttgt cgattttgat
     1501 gctctacaac caaaatggat agtaggttat tcagatatta gtacgttgct acttgctgtc
     1561 acattaaaaa cagggatagc cactgcacat ggaactaacc ttattgattt aagaggagag
     1621 tacttggatg aaacgacagc aatgtgggaa agtgtactga aaacaagaga aggagaatcg
     1681 attctacagc aatcttctga aaagtatcaa aaagtgtggc cccatgagca ttcatcatct
     1741 tctgttttcc atctcactga accaacaaat tggcaatcag tcagcggaga tgcagtaagg
     1801 atgaagggac gattattagg tggttgtatt gatacgatca ggcatttaat cggtactcct
     1861 tatggagatg tccaatcttt taaaagaaag tataatgatg gaaagccgat cttgtggttt
     1921 ctagaaaact gtgagctatc gtcagttgat ttaagaagat cacttgttca gatgaaactg
     1981 gcaggctggt ttgatggcag ctcggggatc atgttcggta gaagtgcggc agaggatttg
     2041 ttagattata aaatgaagga tgtatacgaa gatctgtcaa aggaattaaa actaccgatc
     2101 ttgtatgata ttgattgtgg tcatgtaccg ccacaactga cattgattaa tggtgcctat
     2161 gcagaagtag cggtagaaga tagaaaagcg actgttttgc aaacgtttat ttaaaaaatt
     2221 ttttcctgcc ttggagtcta tcaaagacca ataaatctaa atagaaaaga aagtgaaaga
     2281 agctgacaaa atgctgtcag cttctttttg tataacatat tagaaagtct aattatctat
     2341 aactgtcaca tcgattaact gtttcacgtc attgggattt cctgtaggaa tagctactac
     2401 agatgcatta tcaatgattt cttgagttgt ttctctaatg aacgctggat aagatgtttg
     2461 atctgcaggc atttgccaat gtgaaggaac tggataacta gtgattgtcc cgtcatggtt
     2521 acttgaatac actaaaactc cctggtaact gtatttccca gtgagcataa ccacatcttt
     2581 cggatagact gcactccctg catcataagg ggcgataggt gttcctgcag gataatgaat
     2641 aacatgtaat tcgaaatcac ccgatgtatc tctaaaagta ttacctctaa tagctagcca
     2701 aacacgtgcg tactcaattt gtagatcaga atatcctgct aacggatcta ttgactgaga
     2761 agactgtgag gaggttgtgt ctattgttga agaagaaata tttgtctcaa ctgtactact
     2821 agatgtagaa gaagtattgg gctctgagac tttactgctt gttgaagatg tattcgtttc
     2881 agtagaagct gatttctgag tacttgattt ttcagctgaa ctggtttgtc tgattgaact
     2941 tttgtctgtg gtcgcagatg gatcactatt ttctttacta ggagacgtac atcctacaag
     3001 gaataaagag accaacaata aaataaaacc taactttttc atataaaata cctcattatt
     3061 tttttaatac cagtatagca taatcctgaa tttttgaatc cgtaaaacct tgtctatgaa
     3121 t
//
LOCUS       DAZKBH010000176         3072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000176 DAZKBH010000000
VERSION     DAZKBH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.181"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACG8OO_002759"
     CDS             <1..566
                     /locus_tag="ACG8OO_002759"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9046587.1"
                     /translation="VRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFHAIKNNSV
                     LLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLFEGVPLVG
                     IEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILI
                     KADYNIQTAYKILESKYYELFELIQRG"
     gene            572..1594
                     /locus_tag="ACG8OO_002760"
     CDS             572..1594
                     /locus_tag="ACG8OO_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFP9046588.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            1616..2671
                     /locus_tag="ACG8OO_002761"
     CDS             1616..2671
                     /locus_tag="ACG8OO_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9046589.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT         1229 a          311 c          567 g          965 t
ORIGIN      
        1 atgtacgaat gaaattagga atttcaagtc agacgtttgt aatgggacat attggaagaa
       61 tttcatattc taaaaatcat cgttttttga ttgaaatttt taaagaattt cacgctatta
      121 agaataattc cgtattatta attattggaa ctggtgatat ggaagaagaa ataaaaaatt
      181 atgcaaagaa aagtggcata gatgatgata ttaaattttt aggaaataga aatgatatag
      241 aaaaatttta tcaagcattt gacgtattaa tgttgccatc attatttgaa ggggttccgt
      301 tagtgggtat tgaagctcaa tttgcagatt taccgtgttt cttttcggag aaagttccta
      361 cagaagttgc tttcagtgat aagaccaatt tcataagttt aaatcaatcg gcaaaagaat
      421 gggcgagaga gattgcaaat gtagatatct ctcatcgaga ttcagaacgt tcgattttaa
      481 taaaggcaga ttataatatt caaacagcat acaaaatctt agaatcaaaa tattatgagt
      541 tatttgaatt aatacaaaga ggttaaagaa tatgacacag aaacattcaa gtataaaaag
      601 aattatacat cgcatatccg aagaattgaa tgcaacaaga cgaattgcaa agataacatc
      661 ttggaaggaa gcatttgcca cgttacaagc taaaattgat attcaagtca tgaatcgtaa
      721 tggatataat gaaagtaaaa aacagaagga acatttatta aaaaaacatg aggtaatgct
      781 gaagtattat gaaaagactt tttcagattt tttgaaaaca tatgattata gtcaaaatca
      841 aataattaat caatcaaaat caccatattc taactgcatt tgggtttgtt ggtggcaggg
      901 attggatcaa tcgccggaaa ttgtaaaagc ttgtgtaaaa tcaataaaac gaaatgcagg
      961 gaaacatact gttataattt taacagaaga taattataga caatatgttg atataccaaa
     1021 atgggtggaa gaaaagaaaa acaaaggaat cattacgaga acaaattatt ctgatttatt
     1081 aagattatct ttgctagcaa aacatggagg tttgtggtta gacgccacat tttattgtac
     1141 aaaaccaata ccagatgagt attttgatat cgatatttgg tctattagga ggcctgatta
     1201 tgctcacgca tccgtagcat caggatattt tgcgggttat tcattagcat gtaatcaaga
     1261 gtctagatgg atgtttgcta taattagaga ctttttttta aattattgga aaaataatga
     1321 tatgatggtg gattatttga tgatagatta tatgattgtt ttagcacaga gaataaatca
     1381 tgaaatagca cagacatttg ataaaatacc tagtaacaat ccattatgtg acgaattgat
     1441 taaaaattta aataatatat atgatgaaaa agtatggaac ttattaaaga aagatacttt
     1501 actgtttaag ttgacatgga aacaatcttt taaaaagcaa aaagatgata aagttacttt
     1561 ttatgggatg ttggtagaaa acaaattatt ataaatatta gaggtataaa aagttatgaa
     1621 acacaaggta agtgtgatag taccaattta caatgttgaa aaatatttaa gaaaatgtgt
     1681 ggatagttta ttgagtcaga cattaaaaga gatagaaatc gtattagttg atgatggttc
     1741 gccagatgct tcgggggaaa tagcagatga gtatcaaaaa aaatattcta atgtaaaaac
     1801 aatacataga gaaaatgggg ggttaggacc ggcaagaaat acaggaattg agaatgcgac
     1861 aggagaatat gtggcttttc ttgattcaga tgattgggta gaaagtgata tgtatgaaaa
     1921 attatactta gttgcgtcca aagaaaatgc tgatatagta gttagtggac attgtgattt
     1981 tgcgaatgac aaagctatgg tgaaaaagca acatcctttg agtgggaatg tatatgatac
     2041 gcctaatagt attaaaaatg tacgaaaaaa tttatttgga catagtccaa aagatcaaga
     2101 agtagaggcc ttcccgatgt cagtatgtat gtctttgtat aataaaaatc tgctagataa
     2161 atatagacta agatttgaaa aaattttatc tgaagataca atttttaact tgaatgcata
     2221 tgatgtcgca aataaaattg cttttacaga atatacagat tattgttata gaaaagaaga
     2281 acaagagtct attacaaaaa gtttatctcc aaataagcag acggaattta aagaattttt
     2341 aataactcta aaagctatag catcgaaaga agatttagaa tgtcaaatca gagcaaaacg
     2401 tatggcaatt gattattgta ggctttatgt gggcttagtt gatgatgcaa atttgggaat
     2461 taaagagaaa agaaagtata taaaaaaatt tattgaagat gagggattaa ttcaatgttg
     2521 gaagggatat cctacaaata cgctaccatt tcaacaaaaa ttttttcata aaatgcttga
     2581 atgtaagtgt tatacaatat tgttgttgtt aagtagaatt agaaagataa ttaaaaatat
     2641 gaccagaaaa aaattgttta taatattata aactaaaagt atatcaaata atagaaatta
     2701 acaagttaca aaaaaatatt ataaagaata atattttcta aaaagtaaac tatagtaatg
     2761 tggattatca ccttccaaca gtatataaaa tgagcgtaaa aacggctatt tttgccttaa
     2821 caacgatttt tttgtataca aatacgatat gctcattaat tattggaatt tgtagtgctg
     2881 tttaaattaa tgaaggtgta attatatgag tgatcatttt gaaaaagttt gttgagaaga
     2941 tgtgcttcta gtcatgtgat gggagaaaat aatttaactg ttagagtgaa tagttaaatt
     3001 attctggtta tatgaaaaaa gtagtttcta aaataatctt gcagggatta acttaaagaa
     3061 attgagagaa ta
//
LOCUS       DAZKBH010000177         3068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000177 DAZKBH010000000
VERSION     DAZKBH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.182"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..805
                     /gene="fabI"
                     /locus_tag="ACG8OO_002762"
     CDS             50..805
                     /gene="fabI"
                     /locus_tag="ACG8OO_002762"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9046590.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(915..1967)
                     /locus_tag="ACG8OO_002763"
     CDS             complement(915..1967)
                     /locus_tag="ACG8OO_002763"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFP9046591.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2093..3013
                     /locus_tag="ACG8OO_002764"
     CDS             2093..3013
                     /locus_tag="ACG8OO_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFP9046592.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          893 a          618 c          588 g          969 t
ORIGIN      
        1 tattttttta cgactatcaa atttttatca gaaacaaagg agagtcatta tgtcatttct
       61 agaaggaaaa aaaatcgtca ttatgggcgt cgctaacaaa aaaagcattg cttggggttg
      121 tgctgaagca atgttgaaac aaggggcaga aattatctat acttaccaaa atgaacgaat
      181 gaaaaaatca ttagtcaaat tggtcggcga agaagcattc actgtagaat gtgatgtttc
      241 tagcgacgaa agcatcgaaa aagcaatgaa tgccatcggt gaatatgctg gggagatcca
      301 cggccttgtc catgcagttg cttacgccaa caaagaagaa ttgtccggaa atgtgtctga
      361 tatttccaga gacggctatc aattagctca agatatcagc agctactctt taattgctgt
      421 atcaaaatac gcacaaccgt ttcttgcacc aaacagcggg atcgttagca tgacttatct
      481 aggttcagaa cgcgcaatcc cgaattataa catgatgggt attgcgaaag cttctcttga
      541 agctgctatc cgttatttgg cagctgagtt ctcgcctaaa ggaattcggg tcaatggaat
      601 ctcagctggg gcaatcaaaa cattagctgt cacaggtgtt aaagactatc aaaaactgat
      661 ccagttatct gaatctcgta cgcctgatca gcaaggtgtg acgatcgaag aagtcggtaa
      721 tgcttgtgct ttcttaatca gcccactttc cagcggaatc atcggtgata ttatctacgt
      781 tgacaaaggt gtccatcttt cataaacttt tgttgcattc tttaaagtaa aacatataaa
      841 aatcaggaaa aaacttttta aagtctcttc ctgatttttt ctttttacgt ttttttcaac
      901 tcattacttt gaaattattc cacgacttca atccagcctt ctggtgcttc tatgtctcca
      961 aattgaatac ctgtcaattc atcatacaac cgttttgtta ctggtcctac ttccgtttca
     1021 ctataaaata catgaagatt gttattgtga taaatcccac cgattggaga aataacagcc
     1081 gctgtaccac atgcaccagc ttctgcaaat tgatctagat catcaatgaa aatatctcct
     1141 tcaattgctt gcagtcctag tcgttcttca gctaactgca acaaggaata tttcgtgata
     1201 cttggtaaga tagagggcga ttgcggagtg atgaattttc cgtcttttgt aattccaaag
     1261 aaatttgctg cacctacttc ttcgattttc gtatgtgtgg ctggatcaag ataaacacaa
     1321 tctgagtaat ccagttcttt tgcttctgtt cctggaagta aactcgctgc gtaatttccg
     1381 ccgacttttg ctgcaccagt ccccttagga gcagcacgat cgtaatcaga tacaataaaa
     1441 ttcgttggtt ttagtccacc tttgaaatac gctcctactg gcgtacagaa gacgacaaat
     1501 aaatattctg gagctggatg gacaccaatg ttttcaccga ccccaatcag taaagggcgc
     1561 aagtataatg tagcgcctgt tccatagggc ggcacaaaat cttcatttgc ttttaccact
     1621 tgcttgacag catcaacgaa catcttttca gggaccattg gcatcagcaa tcgtctagca
     1681 ctttgattca tccgtcgact attttgttca ggtcgaaaga gattgacctt gccgtctttt
     1741 cgacgataag cttttagacc ttcaaaacat tgctgaccat aatgaagcgc tggagagcct
     1801 tcatgaatcg tcaggtaatt atcttctgtc aattctcctt cttcccattg tccgtctttc
     1861 catttggcaa taaaacgcca aggagttttg atataagaaa aacctagatt gttccaatcg
     1921 atttgcatat acttaccacc ttcgttattt tctccacatt ttatcattat ccatattatt
     1981 tgtcatttta ttttcagctt ttttccttca ttatagttat ttttgctatt tccttttttg
     2041 aaaacttctt ggtacaatac agaaagaatc aaattttaga aaagaggatt ttatgttttc
     2101 attattagca actgccggta cgacagactc ttctggcgag acattgacag atcaagctgt
     2161 cagcaaagta aatgcgtttc agcggttttg ggacgggatt gattgggacg ccattttggc
     2221 tgcctttatc caaaaaagtt tatatctgat ttttttgatc gtcctttttt ggattcttca
     2281 ccgtgtggga aaatatctga ttgataagtc ttttaatcag tattctaaaa aagccattct
     2341 aaacgagagc cgcttaaaga ctttacatag tcttttaaat actatttttc attataccct
     2401 tggtttcttt tttatctatg caattttatc tgctatcggt gtgccgatcg gatcactttt
     2461 ggctggtgcc ggaattgcag gtgtggcaat cggtcttggt gcccaagggt tcatgagtga
     2521 tgttattacc ggatttttta ttatcatgga acaacagatg gatgttgggg actacatcaa
     2581 gctagcaaac ttaacgattg aaggaaccgt cgtatctgtt ggtatccgta cccttcagct
     2641 gaaagcaacg gatggtactg tccatttcat cccgaatcga aacatcacca cgatcagcaa
     2701 tctctctcgt gcaaatatgc aagtactttt agatattcgg attgttcctg aagaaggata
     2761 tgacaaaatc tatgagatca ttgatcgagt aaatcgtaca ttagctgaaa aataccaaga
     2821 agagctccaa actgaacctt ctatctttgg attagtggat cttggaaaca gcaattttgc
     2881 gattcgtacg gtctgctacg cattgaacgg aaaacaatat gccttaaaag aagagttctt
     2941 gagctcctat gtcaaagaat tgacagcttc tggtttcacg attccgaaca gtccaattgc
     3001 tgtcggaaaa taatttggac aaacgatgca acgaatctca ctaaaaagag cgtgggacaa
     3061 aagtaaag
//
LOCUS       DAZKBH010000178         3053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000178 DAZKBH010000000
VERSION     DAZKBH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.183"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..353)
                     /locus_tag="ACG8OO_002765"
                     /pseudo
     CDS             complement(1..353)
                     /locus_tag="ACG8OO_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(613..1161)
                     /locus_tag="ACG8OO_002766"
     CDS             complement(613..1161)
                     /locus_tag="ACG8OO_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046593.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACG8OO_002767"
     CDS             complement(1162..2019)
                     /locus_tag="ACG8OO_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9046594.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACG8OO_002768"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACG8OO_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACG8OO_002769"
     CDS             complement(2581..2910)
                     /locus_tag="ACG8OO_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9046595.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..>3053)
                     /locus_tag="ACG8OO_002770"
     CDS             complement(2987..>3053)
                     /locus_tag="ACG8OO_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9046596.1"
                     /translation="KVDEDEHVVEIYSARTHYESQ"
BASE COUNT          893 a          620 c          384 g         1156 t
ORIGIN      
        1 aaatgttctt actgcctctt ccacactaat ttcttcgttc aatttcacta ataatacttg
       61 atctcgctta ttataatcgc tttcatttat cctaagagtc aaatcttttt ccaccataac
      121 ttttacaccc aataaatttt gcggtttatt tttctctgtt gaaattcttt gtgtagcctt
      181 taaactgaca attagctcat tgggtacata cttatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc tta
//
LOCUS       DAZKBH010000179         3012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000179 DAZKBH010000000
VERSION     DAZKBH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.184"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..359
                     /locus_tag="ACG8OO_002771"
     CDS             177..359
                     /locus_tag="ACG8OO_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9046597.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="ACG8OO_002772"
     CDS             462..746
                     /locus_tag="ACG8OO_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046598.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="ACG8OO_002773"
     CDS             762..998
                     /locus_tag="ACG8OO_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046599.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="ACG8OO_002774"
     CDS             1020..1274
                     /locus_tag="ACG8OO_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046600.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="ACG8OO_002775"
     CDS             1356..1541
                     /locus_tag="ACG8OO_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP9046601.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="ACG8OO_002776"
     CDS             1608..2003
                     /locus_tag="ACG8OO_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9046602.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            2020..2370
                     /locus_tag="ACG8OO_002777"
     CDS             2020..2370
                     /locus_tag="ACG8OO_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046603.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="ACG8OO_002778"
     CDS             2392..2766
                     /locus_tag="ACG8OO_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046604.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DAZKBH010000180         2910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000180 DAZKBH010000000
VERSION     DAZKBH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.186"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            798..1052
                     /locus_tag="ACG8OO_002779"
                     /pseudo
     CDS             798..1052
                     /locus_tag="ACG8OO_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="ACG8OO_002780"
     CDS             1122..1634
                     /locus_tag="ACG8OO_002780"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9046605.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="ACG8OO_002781"
     CDS             1631..2668
                     /locus_tag="ACG8OO_002781"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9046606.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            2828..2910
                     /locus_tag="ACG8OO_002782"
                     /pseudo
     CDS             2828..2910
                     /locus_tag="ACG8OO_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         1051 a          517 c          580 g          762 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgac catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc
     2881 ctctacaacg attaaaggca tggaagccat
//
LOCUS       DAZKBH010000181         2907 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000181 DAZKBH010000000
VERSION     DAZKBH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.187"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..113
                     /locus_tag="ACG8OO_002783"
     CDS             <1..113
                     /locus_tag="ACG8OO_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9046607.1"
                     /translation="SVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(199..1293)
                     /locus_tag="ACG8OO_002784"
     CDS             complement(199..1293)
                     /locus_tag="ACG8OO_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFP9046608.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1482..2243
                     /locus_tag="ACG8OO_002785"
     CDS             1482..2243
                     /locus_tag="ACG8OO_002785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9046609.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2358..2798)
                     /gene="fabZ"
                     /locus_tag="ACG8OO_002786"
     CDS             complement(2358..2798)
                     /gene="fabZ"
                     /locus_tag="ACG8OO_002786"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9046610.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          933 a          574 c          484 g          916 t
ORIGIN      
        1 ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac agaacaccaa
       61 tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt tgacaaatca
      121 taacctataa aaaaacaaaa taaaaaagca gaaataactg gctatttcta gcggattatt
      181 tctgcaataa ctaattatct attttttatt ttcaattaat ttaaagcttg accatggttt
      241 caaaaagtca aggaggaata attcgtcatc ggagattctt cctacaacat tgacacgtcc
      301 gtcgtttttc atatccttca aggcgatttg tgtttctcct ttgtattgac cgtacccttc
      361 attgtcaatc agtacatccc catgatgcat atctcgcgta ttatgaggcg ggaaatcttt
      421 atctttgtag tatacacgag tcatcgttga acgaaggata tattctgagc gatcccctcg
      481 ataactatga aggttatcaa acaagcagat tcgttcgttt tctgtaatac cttcttccgt
      541 atctactttc aatgttggat aatccgcgtt aaatgcttct gccatttctt tcaattcttc
      601 ttcacttgcg tacgcatttc caatagaaat atcatcaatc aatcctgtaa ggattaaatg
      661 cttcacttgt gtcgcaattt caagatctcg gtggtcttcc agcgtgcaca agccatcttg
      721 cgtaggccaa gggccaaact cagcagattg cgaattgacg aatgccatcg tattcaaatt
      781 atattttctg aatttttctg aacaaaaaac aaagtgttcg tatcctaaac cagagtatcg
      841 atgaggataa aaattatgag aacctaataa attatccgtg tttggcgaat aactcatgat
      901 gttgtcaacg taattcgtgc ccgaactcat attgatctcg attttgatac caaaaggatt
      961 gcgcgtcatt tttgcttctt ctgctcctgt gaatccgata tctaagcgga ccccgtaggc
     1021 acccatctta tggaaaaaag ataagtcatc gtaagaaatc tccaactgtt caaataacgc
     1081 tggattgata tcaaccatta cttccatgcc taggctattc gcgtaatcaa ctacttcttt
     1141 aaattctgaa agaacttttt ctttatcatc attgatctgc agcaaactgg taaaaacccg
     1201 tttataccca tacttatgag ctaaatccaa gtacgctttg tccttttcaa atgttgaccg
     1261 ttctggataa atcgagattc ctaattttcc catattacca tttcctcctg catcttttcg
     1321 tttttgtatc cacttacatt atagcaaaca caaagtaaat ggtctacacc ttttcttttt
     1381 cttttaaaat aaatgataaa ttccaataat ttaagtaggt attttccctc tattttgtta
     1441 tcattaaaga taacaaaata gtaaattgga aaggaacagt tatgaagaaa aataaagtta
     1501 ttttaagtat tagtttagca gcagcattgc tgtttgctgc tggttgttct gcaaacaaca
     1561 cgacttcttc tagttcgtct acagaaagtt caaaaagtac atctactgcg gaaagcacga
     1621 ctgccagtag cgtcgatttg aacgcattgg aattaccaca attgacgaaa gatgtcgccg
     1681 aaaatgaaga tctggtcgaa atggtgacga ataaaggcac aatcaaaatc aaattatttc
     1741 ccgaacacgc accaaaagcg gttgaaaact ttatgacaca tgcaaaagac ggttactaca
     1801 acggactgac tttccatcgc gtcatcaaag atttcatgat ccaaggtggc gatcctaaag
     1861 gcgatggcac tggaggggaa agcatttggg gagaaggatt tgaaacagag atcaacaacc
     1921 acctttataa tataagaggt gcgttatcta tggctcgctc acaagatcct aacagtaacg
     1981 gcagccagtt ctttatcgtt caaaatacag atgatatgca tgatggtctt ttaaaagacg
     2041 actatcctca agcaatcatc gacgcttata aaaacggcgg ttatccttca ctagatggcc
     2101 attatactgt cttcggccaa gtgattgaag gaatggatgt cgtggatgct attgcaaata
     2161 tcgaaacaga tagttcagac aaaccaaaag aagaagtaaa aatcgataaa atcaatatac
     2221 tgcaagaagc aaaacaaaaa taaaaaaata acgtctgaat cctgcataga tgacaacgta
     2281 tgctaggatt caggcgtctt ctttttacat aaaaagcaag taagcttcct tttgttttgc
     2341 tatatcaggt tttatagtca agcattttgg ttgacgatga acgtgaactc gcactcacag
     2401 acttttttat catctacata tgctgcagat aacgctgtac cgactgatcc cttcattttg
     2461 gtgatttcaa aatgacattt cagcacatct cctggaacaa ctttttgacg gaatttggcc
     2521 ttattgatcc caccgatata agctcttttt cctagaaact cttctgattt taggataagt
     2581 atcgaacctg cttgtgccag tgtttctaga atcaacgcac ccggcatcac tggattgtca
     2641 ggaaagtgac cttggaaaaa atcttcattg attgttacat tttttgtcgc aacgatcttt
     2701 tttcctggtt ctaaagtatc tacataatca atataacaaa taggataacg attaggaatc
     2761 aattccatta cttcagtagc agataatacg taagacataa ttaaactcct tctttcaggc
     2821 aattgccatt ttcattactt tgactatcaa agtatcatat tcctaaatat ttagcaatct
     2881 ttttttaatt taagaaaaaa tagtttg
//
LOCUS       DAZKBH010000182         2766 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000182 DAZKBH010000000
VERSION     DAZKBH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.188"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1037
                     /locus_tag="ACG8OO_002787"
     CDS             <1..1037
                     /locus_tag="ACG8OO_002787"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9046611.1"
                     /translation="LEIISAYNFENISILKFDSRFKELFNVFKKQNKLLTLGSCLAQV
                     IFQLWIGGIFLIDVILLAKGTISITNFVIVFMYANMVPTLWTTLISIIWKWQDYSVSL
                     NRYYEYYSNNNKVAGTKKINKINSITGQSLNFSYNKRKVLSNISFSTEMNKLFMISGE
                     NGTGKTTLMKLFTKQLPVSNNQLFVNGIDINMIDNESLRRLISIATQIPYIINSSVKE
                     NILMGTKNYDKKKFSKLIELLGISKFMTKLGITLDDNIYDEKILLSGGEKQKISLARA
                     LIKSASVIILDEPFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEIL
                     YLNKEYDNNE"
     gene            1030..2058
                     /locus_tag="ACG8OO_002788"
     CDS             1030..2058
                     /locus_tag="ACG8OO_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP9046612.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            2114..2332
                     /locus_tag="ACG8OO_002789"
     CDS             2114..2332
                     /locus_tag="ACG8OO_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046613.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            2403..>2766
                     /locus_tag="ACG8OO_002790"
     CDS             2403..>2766
                     /locus_tag="ACG8OO_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046614.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNES"
BASE COUNT         1093 a          303 c          477 g          893 t
ORIGIN      
        1 aattagaaat tataagcgca tataattttg agaatatctc tattttaaaa ttcgattcta
       61 gatttaaaga attatttaat gttttcaaaa aacaaaataa attactaaca ctgggaagct
      121 gtttggcaca ggttattttc caattatgga ttggaggaat atttcttatt gatgttattt
      181 tattagctaa aggaacaata agtattacta attttgtaat agtatttatg tacgctaaca
      241 tggtccctac tttatggact actttaatta gtattatttg gaaatggcaa gactattcag
      301 tgtcattaaa tagatattac gaatattatt caaataacaa caaagtggct ggcacaaaaa
      361 aaataaataa aataaattct attactggtc aatcattaaa tttttcatat aacaaaagaa
      421 aggtattaag caatataagt ttttcaactg aaatgaataa actattcatg atatcaggag
      481 aaaatggaac agggaaaaca acactgatga aattatttac taaacagttg ccggttagta
      541 ataatcaatt attcgtcaac gggatagata ttaatatgat tgataacgaa tcattaagaa
      601 ggttaatttc aatcgcaaca caaataccat atataattaa tagctccgtg aaggagaata
      661 ttttaatggg gactaaaaac tatgataaaa aaaaattcag taaattaata gaactattgg
      721 gaataagtaa atttatgacg aaattaggta ttactttgga cgacaatatt tatgatgaaa
      781 aaatattatt atctggagga gaaaaacaaa aaataagttt agctagggcg cttattaaat
      841 cagcttcagt tattatatta gatgaaccat ttagaaattt agataatgaa actcgcgata
      901 atttgaggga ttatatacta aatacaaaaa aagaagtttg ttatataatt ataactcatg
      961 atcacattct agataatttt ttggattcaa atgatgaaat attatattta aataaggagt
     1021 atgataataa tgaatagcaa aaaacttcag atgggtgaaa ttgttagagg tcctagtcaa
     1081 atatctattg atgttacata taaatgtaat tttagatgta aacattgtta caatgcgagt
     1141 ggtggagaca attgttttat ggataatgag ttgactgatg atgaactgtt agaacttaca
     1201 aaagatatat gtaatttaaa gccatataat atttgtttct gtggaggaga aactttactg
     1261 agaaaaaaaa tagttttatc ttgtgccact ttattaaaag aggcaggtat tcctaatatt
     1321 gctattgtta ctaatggatc attagtaact caggatgttg caaaagaatt attcaaagca
     1381 ggtattaatc gatttcaagt aagtttagat ggaaaaaatg ctagtagcca tgaagcactt
     1441 agattatatc caaacagttt tgaaaaggca attaatgcta tcaaattatt tagagaattg
     1501 tccgaagaag tggatgttgc tttttgtcct actagtttta attacaatca attacgagaa
     1561 gtatatcaac tagtaaatac tcttaaagtg aagtctttaa gaattcaacc atttatgctt
     1621 agtggtaggg gaatgcaaaa tagggaaata gtgccaactg tttctcaata tagaaaacta
     1681 gtttctgata ttaactatat taaaagaaat gaagattata ccaagataga ttggggagat
     1741 ccaattgatc atatcatccg acacagaact ttattgaaag atttttcgaa ttttgtttct
     1801 attaggtcag acggaggatt aatggcatca ccttacttgc caattcaagt tggaaatatt
     1861 aaatgtcaca gtctttctaa atattggcga gcaggacttt ataaagtttt ttctttagat
     1921 ttagttcaag atatagctac tcaagtttct tcaatacaag atatgagtaa agaattaaaa
     1981 aaaggacata ttaaatggct taatgaagat atagaaattg atattctcga tgaatacaaa
     2041 catgatattt taatttaggt ttatcattgg aaagtttaga taaacaaagt acttttagaa
     2101 aggagggaaa aaaatgattt ctgaacctca aatgaataat atgtatgaaa gcccaactat
     2161 aatgggagct ggtgaagctg cgccaagagc ttgttcagca attatggtgt gtgtgttatt
     2221 agcatctgca ggagtaggta tccaagcagg agcggtgtat acttatgctg tagttgctac
     2281 agtagctgta gctgcagccg cagtttggaa aacagtagga ttagttggtt aggaattaat
     2341 aggagactaa ctgtctccta ttgattccaa tgattaagct aaggaggaat tttaagaaaa
     2401 taatgaaata caataattta aaaccgaaat tacttaatga gaacatagaa tatataagag
     2461 aagaaaatta tggatattta accatagttc ccaaaaatgg aactgaattg aaagataacg
     2521 ttttcaatcc aacagtaaga tatatattgg ctaactgtac tggaaaaaac agtgtaaaaa
     2581 aaatagcgaa tggtttaaaa aagaagtttc ctagtttaga atatgaaaaa ttctatcaag
     2641 atgttgtagg aactattaaa tttttcaaac actataatat tattgatata ggagattata
     2701 gtatggaaga aaagaaaata tataaaattg aacaaaattt agaattagaa ttactaaatg
     2761 aatcac
//
LOCUS       DAZKBH010000183         2688 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000183 DAZKBH010000000
VERSION     DAZKBH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.189"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1350
                     /locus_tag="ACG8OO_002791"
     CDS             <1..1350
                     /locus_tag="ACG8OO_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFP9046615.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>2688
                     /locus_tag="ACG8OO_002792"
     CDS             1484..>2688
                     /locus_tag="ACG8OO_002792"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9046616.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGL"
BASE COUNT         1056 a          368 c          532 g          732 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactatt
//
LOCUS       DAZKBH010000184         2672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000184 DAZKBH010000000
VERSION     DAZKBH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.190"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(261..545)
                     /locus_tag="ACG8OO_002793"
     CDS             complement(261..545)
                     /locus_tag="ACG8OO_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcQ family protein"
                     /protein_id="HFP9046617.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
     gene            complement(566..1969)
                     /locus_tag="ACG8OO_002794"
     CDS             complement(566..1969)
                     /locus_tag="ACG8OO_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HFP9046618.1"
                     /translation="MTASDLIRNFIMMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDF
                     FRYYLAAITGEYSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPE
                     GKYSEVLSDFQSMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNG
                     DDTSRVSKAFPSYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNL
                     LNANAYAMRLARWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYT
                     GLVNTIGNLTLVTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISAR
                     SEALINELITMYPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYS
                     KIKDIASDSLDETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDY
                     WKYDNGISINDSLRNKK"
     gene            2155..>2672
                     /locus_tag="ACG8OO_002795"
     CDS             2155..>2672
                     /locus_tag="ACG8OO_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFP9046619.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPIT"
BASE COUNT          826 a          545 c          398 g          903 t
ORIGIN      
        1 tatgtgaaaa tcgaatcttg agatagctat tccccttgag atattcactg atacttctac
       61 tcaaatcatt taaattttta taattatttg tacggaacgt atatatatca acatctattt
      121 agacgttgtc ggtgatacaa tgtcattttt cccgtgtact atgtcattta tctctatact
      181 ttgtcatttt tctttttggg caatcaaaaa aggcaacaaa ccaattatga tttgctacct
      241 tctccgttta aaaacttatc ttagccccaa aatggaatcc aatttttaac ttcttttgcc
      301 aatgcatacg ccttagcaag acctgagttt tcaactaaat atttttcccc ttccagagaa
      361 acactccccc agatatgaac agtgttatcc gcccaaacca tggattctag taagccattg
      421 cgcgaaagaa atttagtttg ttctaaaata tcttcttctg gaacttgccc tttaagctca
      481 ataaaaggat tctttttttc atcacgcaat ttaagaattg catatcttag tttgttctta
      541 tccaacctaa caccacctct atgctctatt tcttatttct tagcgaatca ttaatactta
      601 ttccgttatc atatttccag tagtcccaac cattccggct accccctaat agcagagctg
      661 ctgcattcga aacagatgag gcagtgtaat cttgaacaaa ttgtagtccc ccaattgttt
      721 cttcaatgat tccattatcc aacagctctt ggcgagtttc atctagcgaa tcagatgcaa
      781 tatctttaat cttagaataa atttctgaac ctgaatgtat aatcacggtc tcgtcttcgt
      841 tcaaataccc agttgctttt attccttgcg cctctaagaa aatttcccta ttcccatcgt
      901 gttcatagtc gcccgacgat ctcaaatagg gatacatagt aatcagttca ttgattagag
      961 cttcacttct ggcagaaata tccgatgatg tccactcagt caattcgtac aaatctttat
     1021 tcatacgaat atgaagggta tcttcaaaga ttttcttttt ggtctcaaag tctttatttc
     1081 ccgcggccga attatcagga tttgtaacga gtgttaaatt accaatggta tttactaatc
     1141 ctgtgtattc ttcccccgat gtaccagcct tctcttccca atatgaagta ctagtctgtg
     1201 gcatgatgtg ttcaatggac aagttactca tatcaagtgt tgctgaattt tctcgattct
     1261 ctattttttc aagtaaccaa cgggccaatc tcatcgcata agcattggca ttcaatagat
     1321 tagatttcaa tgccttgtca gatggaagtg ccatattatt tgattgattt ctagtaacaa
     1381 gaacataaat tacaatatca acgatatttt catatccatt agctttagcg tacttcctaa
     1441 cacttttcaa atatgaagga aatgcttttg acactctgct tgtatcatcc ccattaaaat
     1501 atctcctaag ctgataatca ttcactacct taatcgcatc aatatattgg tcttcagtga
     1561 taaattggtc atgatagtac cattcagata atcccaaaac aaaaggtgca ggcatcatac
     1621 tttccatact ttggaaatcg ctcaatactt ctgagtattt tccttcaggt ttgttatagt
     1681 aaagtctcgc aaaataacca gcatatcgca ctaatttttg gagcaattcg gcatcacttg
     1741 atacatcgcg ctctttatgc caatagtctt tgaatgcttg ataaagaata tgctttgctg
     1801 aatactcccc agttattgca gcaaggtagt atctgaaaaa gtcttccatt cctttagaat
     1861 tatcaaagac ttcttccaac ctacgccaat atttagtata aagcgtagtc tgctcttcat
     1921 tagatttatc catcatgatg aagtttcgaa tcaaatccga ggcagtcaat ctttcaccgg
     1981 tagaattgat actttcaaaa atttgctgtg cattatcacg gtcatctaat ttaatataaa
     2041 caatacgcgt ggtgctagtt aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa
     2101 tcaacaaaaa acgcatatag tagtctcaaa actcccaaga aatgaggact acatatgcgt
     2161 tcatcaaaat atactgaaca ttatacagat acttttataa cattcaagga aattatgaga
     2221 actgtttcta acatatacca caattgtgta ccagataaga ttaaaaaccg aagaaatact
     2281 gatcaactga agcaacgcga tacagtgatt attgcttgtg ttatatgggg cataattaat
     2341 ggctatacta gccaaagagc cacgtataga gcggtttgtt ccgtcttatt tcttaatggt
     2401 gactttccta gtaggagtcg attcactcgc ttaagttcaa acttagctta cactatcaag
     2461 attattcgat actttttcat caagaagctc accaaaggtg aactagtcgg aattatagat
     2521 agttttccta gtccattatg taaaccagtt agaaatagac aagcaaaact attgaatcaa
     2581 atagccaaag ttggctataa ctcaacaaaa aaatcttatt tttatggtct taaaattcat
     2641 atgatcgtta ctaagactgg ctttccgatt ac
//
LOCUS       DAZKBH010000185         2671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000185 DAZKBH010000000
VERSION     DAZKBH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.191"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..321
                     /locus_tag="ACG8OO_002796"
     CDS             <1..321
                     /locus_tag="ACG8OO_002796"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9046620.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
     gene            735..1553
                     /locus_tag="ACG8OO_002797"
     CDS             735..1553
                     /locus_tag="ACG8OO_002797"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9046621.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1626..1985)
                     /locus_tag="ACG8OO_002798"
     CDS             complement(1626..1985)
                     /locus_tag="ACG8OO_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9046622.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          925 a          372 c          471 g          903 t
ORIGIN      
        1 tataaagcaa tcattcctga gaacttagat gaggttaaac tttatttatg ggaagataac
       61 ggagaaattg tcggttttag tggtgtaagt gcagaagaat tagttatgct atttcttgat
      121 ccagatttta ttggtaagcg atatggtagt caaatattaa ttgagctgat tgaaactgaa
      181 gatataaaaa gaattgatgt taatagtcaa aataaatatg caaaacattt ttatttaagc
      241 catggatttg aaattgaatc tgaagaagac ttagatggtt ttggtaaacc ataccctata
      301 acgcatttag taaaaaaata attcatgaat ggtttattac ctgattgaac atgaataaaa
      361 gtaatatttt tattttgatg gcctatttct aggttatgaa ttatccagat caaatttgtg
      421 tattacattc agtaataagg tgcttatctt aggatcaact tatgatacta gctgaccaag
      481 gatgtaccag ttatagaaaa tcagttaaag taaaaatgat aaaaatctta tgtcaacact
      541 gatttaggct gacctgtggg ttaggagaaa gacaggtcgg tagcttttaa tacaaaaaat
      601 ggcttgtatg tatctagata gttaactgaa attagaaatg ttcttatttt taacggagta
      661 agaaaaatag gctgctttag gtctgaatac tttttcaata taatgaagat atcaaaggcg
      721 acaggagtgt ttttatgaat aagaatcctc ataatctaaa gttggtagca gtagatatgg
      781 atggaacatt tgctcgtact gattacacat acgatatccc aaggttcaag gctgttttat
      841 cgcgaatgaa agctgttgat tgtcaatttg ttgttgcaag tggaaatcaa tactaccaat
      901 tgagatcgtt atttccaaat tattatgatg aattaagttt tgttgctgaa aatggtgctt
      961 ttgttaaaga taaaacagag gtactatttt cagccgatat gcctagaaaa gcgatcgagg
     1021 tggtccttga tttatgtaaa gactatcctg aagtaaaaaa tgtgatgtgt ggtctgaata
     1081 gtgcctactg tgaaagagga aaggtgagtc aagcattttt tgatctaacc aatatctatt
     1141 accatagatt agaatgggta gctgattttg cagaagttca agataaaatt ttaaaatttg
     1201 ctccgactat gcctgttgaa aaaaccgaat tttacgttga attgttccgt gaaaaattag
     1261 ccgggctatt agtacctacc agtagtggtc atggttctgt agacttgatt ctttctgagt
     1321 gccataaaac tttcggcttg aaaatgttag ttgagcgctt ggggattaat cctgaccagt
     1381 gcgttgcttt tggagatggg ggcaatgaca ttgagatgtt ggagtattgt ggtctgagtt
     1441 atgaaatgga taatgcaacg gaagctgtta agcaagttgc taagcatcaa tgtccttcaa
     1501 atgacgaaga cggtgtttta gtgacgctcg atcgattgtt ccctaacacg taaacactag
     1561 gtaattcatc aaaaaacctc gatataagaa atattcgagg ttttaaaact atatttagaa
     1621 cagatttacg aagcgtctga tgagtcaaaa tagacttgga cattgctatc taaaatacca
     1681 ctttcattct ttaaacgaat tgtttgattt gatggagtta cctctaatac taaatgccca
     1741 ttaataaagg tagtatcgta ttcagatgtt ttaactgttg cttcttcgca aggtattgtt
     1801 aaatcatatg ctggaatata atgttgtgtg gaaactaata aaaaatgatc accaatcata
     1861 cagcatccct ctgctgaaga aaattgatta gagccatcat ttaaagttaa caagaatcca
     1921 tcctttaaac gaggtacttg cttaaatact tctggttgta cttctaaaaa taaattatcg
     1981 ctcattttca cgaccaccat cgacttccac agttgaaatt gacattttat ttaacacacc
     2041 tattccccct taactataac tcaattctat ataaaaaata ttctattctc aaataattga
     2101 acttttcaaa tcgccaattt acttcggctt tatcagagct cattcatatc aagagcgatt
     2161 aacgctgtaa aaattaaacc gaagatctga taaattttct ctattttcta caatatttaa
     2221 aacgcttcct tagtctatta tatatctatt tctcttagga gtatgtttag atgtttgaat
     2281 aatagttgcc caactttaaa ggttattctt ataatatttt acttcatttt ccaatagaaa
     2341 attcaaagga ttactggttt ttctaaaata gtttagttta aatacataag tatcctccga
     2401 cactgtatta gttcataatc atctaaatac aaaattttca aataaaaata tttcaagctc
     2461 tatcgttgac gaatgtaaac gatacttata aagtaataga aattgattac atttgtaaac
     2521 ttaaaatggt taatttatct cacataaaac taagaataaa ataatgatta aaagtggtta
     2581 gtttttatac gatttagcct atcggtaaaa atgaattaga gagtgtaaaa tattttgtgt
     2641 aaatgaaaaa atccatacaa aaaaggaagt c
//
LOCUS       DAZKBH010000186         2670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000186 DAZKBH010000000
VERSION     DAZKBH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.192"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..101
                     /locus_tag="ACG8OO_002799"
     CDS             <1..101
                     /locus_tag="ACG8OO_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9046623.1"
                     /translation="SGAYLSDILNGNRDGKKAQQHIETVKKILDIR"
     gene            113..361
                     /locus_tag="ACG8OO_002800"
     CDS             113..361
                     /locus_tag="ACG8OO_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046624.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            392..571
                     /locus_tag="ACG8OO_002801"
     CDS             392..571
                     /locus_tag="ACG8OO_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046625.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(614..1084)
                     /locus_tag="ACG8OO_002802"
     CDS             complement(614..1084)
                     /locus_tag="ACG8OO_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046626.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1171..1869
                     /locus_tag="ACG8OO_002803"
     CDS             1171..1869
                     /locus_tag="ACG8OO_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFP9046627.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2047..2388
                     /locus_tag="ACG8OO_002804"
     CDS             2047..2388
                     /locus_tag="ACG8OO_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046628.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2381..>2670
                     /locus_tag="ACG8OO_002805"
     CDS             2381..>2670
                     /locus_tag="ACG8OO_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFP9046629.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          983 a          389 c          538 g          760 t
ORIGIN      
        1 tttcaggagc ttatttatca gatattctaa acggcaatcg tgatggaaag aaagcacaac
       61 agcatatcga aacagtgaaa aaaatattgg acatccgata ggggggtagg cgatgacaaa
      121 actaaaaaaa caagattttg taaaaaaata caattattct ccatctactt atcaacgtcg
      181 aatgtcggaa ctaaaaaata cagcaatttt ctcagcggcg tatgaacggg tcacaggaca
      241 agaagtttgg atcaatacag aattatacga taaatttttg tctttcaaat cctataacag
      301 gttacgcaca agaaaggtaa cacctaaaga atttatcgag aagcatttag ttgatttata
      361 aaaaacagaa cattttgaga ggtgaaggtt aatgggtaaa ttcaacagag cattggtatt
      421 cagcgcaccg ctaatcatct acgctttagg actttgggga agcaggcaag cgttgatagg
      481 aacgattgtt tacatggttt ggatttttat ggggcttgat gaagctgaag ctgagtacag
      541 agcgaaaaag ccaaccgagg gggctgacta aaaatgaata gaaaagagaa actagaatgt
      601 atattactat tactcagttt aattctttca ctaatttctc tattgggaag tttgtatttt
      661 tgatatcaac aattttgtgg atttgggttc tatttagagt aaatatcatt tttgtttggt
      721 gattaaggag tttattaaaa ttaattttcc ctacttcaaa tgcaaggtaa caagatatag
      781 cacttttagg aggaatattt agcggtacag cttcagagta tattctatta tcatcgactg
      841 atcctccttt agcaattaat ttcttaaatg gagtagctga ccatctgcgg tttaagttag
      901 cggatgaaag ttctaaatta actaacatag ctggttctgt tgaatagtta gaaatgatta
      961 cttttgtata aatcatatct gcagcgaagt aagaagcatt aagttcaact tctagttgag
     1021 ggcgcattcc ttttattttt atccctgtta aaaacgtact caatataaaa cttattattg
     1081 acatccattg aaaaattgtt aagtgaaaaa aattcaaaat aaacaccacc agtttttaac
     1141 taaattatac caaaaaggag agaagaaata atgcaagaat tagtaatttt gaaaaataaa
     1201 gaagctgtga ctacgagctt acaagtggca gaaagcttcg agaaaaaaca tcaacatgtt
     1261 ttaagagata ttgatgcact aaaaaaagat gtgtccaatt ttggacagat gtttgtagaa
     1321 ggtaatgaac cagattcata tggcagaaat cgacgagttt tcttcattag tagagatgga
     1381 tttttcttgc tggctatggg gtttacagga aagaaagcta tctacttcaa acaaaaatac
     1441 attgaagcat tcaacgaaat ggaagatgtt attcgcaaga atactgttcc tcaaacaatt
     1501 gaagatatga tgatctatca attagaagaa atgaaagatg ttaaaaaaga tgtttccatg
     1561 cttaaagata ctatgcgaat tagcggacaa caagagtttg aaattaagca aaaaggaaat
     1621 atgaaagtta tgggagttct agggggaaaa gaaagccgag cttatgaaga aatcagcaaa
     1681 aaagtattct caaaattttg gtctgaattt aaacgtacct tttcaatccc aagatatggc
     1741 gagttacctc gtaagagatt cgatgatgct gtttcattta ttgaaatgtg gttgccagaa
     1801 actgcgatcc gtatggaaat tgatcaactg aacagacaac agagactttt tggtgatgac
     1861 aatgaataga gctgaagcgc taagaatagg gacggcaatt gctaattgct ggtggaaata
     1921 ctataaacca atcatcctaa gccaacaaca tattgacaag caaaaagcat ggcaacaaat
     1981 aaaaaagtga ctccgccggc aagcaaagag tcacaaagaa aacacatcat aaggagattt
     2041 tagcatatgg aaaaagaact ttccactcta gatcaatatt tgattgatcc tgattggggc
     2101 aagccgaaaa ttgaggaaac aagtggtcga aaaatcagac gaaatctttt gacgaatgaa
     2161 gaactagctt gggatcaaga tgatttaggc aaccatgtaa ctatttggga tcatgtttat
     2221 cttatccatc tatcgaagca ttcgaataaa cctgaatata tttacgtcat cgaagatggc
     2281 ttgattgatg cgctagaaga gtacgacaga gataacttga ttgatatctc ttattacgga
     2341 ccaggtaaga aatacattgc tgaaatggag gcagaatttg atgagtgaaa tcaaagggac
     2401 aacgaacttt gaaaaacttt ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa
     2461 ttttgattat ttatcttggg ctcacgcgtg ggagattatg aaaaagaatg atccacaggc
     2521 aacggtaact attaatgagt acaaacacta cagggttgtt tctggaactc atcaagactt
     2581 tcttgttgag gaatataaac cttttcttat ggatgaaact gggacttatg tatctgtctc
     2641 agtaacggtt aaaggacaca cggaaaccga
//
LOCUS       DAZKBH010000187         2574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000187 DAZKBH010000000
VERSION     DAZKBH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.193"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..825
                     /locus_tag="ACG8OO_002806"
     CDS             <1..825
                     /locus_tag="ACG8OO_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9046630.1"
                     /translation="DLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVL
                     FNQFKIDGKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQ
                     YRTKGIAYFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGR
                     GKPVIGYQFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQ
                     KRKLELLQQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            835..1332
                     /locus_tag="ACG8OO_002807"
     CDS             835..1332
                     /locus_tag="ACG8OO_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9046631.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            1708..1989
                     /locus_tag="ACG8OO_002808"
     CDS             1708..1989
                     /locus_tag="ACG8OO_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046632.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            1991..2407
                     /locus_tag="ACG8OO_002809"
     CDS             1991..2407
                     /locus_tag="ACG8OO_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046633.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1041 a          453 c          390 g          690 t
ORIGIN      
        1 gatttaagca attataaagc aactgcaaat gttcgattca tagatgattt agaaacaacg
       61 tatgacaaat taatggattt acgttttggt agaagaagtg ctgacggttt gcaacgtgaa
      121 cgatttgttt tatttaatca atttaaaatc gatggaaaag ctgatattcc ttttgctgaa
      181 atacaagtcc atgagaaagc cttaccctta cttaataatc ttgaagaatg ggttcgatat
      241 tctctccaac aattcaatga attagaaagc agttattcca aaaccatgtt tagattatta
      301 aaacaataca gaaccaaagg aattgcctat ttttcaaaag aagactttca tgaattactt
      361 gatatcccta aaagttatag agaaagtgat ataaataaaa aaattataaa gcctattcgc
      421 caagaactaa ccgcaatctt caaaggtctg acaatcaaaa agaaatacgg aaaaggaaga
      481 ggaaaacctg tcattggcta tcaattcacg tttaaacctg aaataaaaaa agcagacgac
      541 tttaataaac acgcaccaac aaaagaagaa caaaataaag ctttagaact agttaatcaa
      601 caaatttact ctgaaaaaga ccccacttca ttacaagaaa aaatcaaaga acagaaacga
      661 aaattagaac ttttacaaca attagaagag ctagaaagaa aacaaaataa tctgaataaa
      721 gaaaataagg agattaacga acaaatacca gataacaatt ccgatattcc cccagaatta
      781 aaaaaaggct tactagataa tcttcaaaac ttatttaaag aataaggggt ttttatgaca
      841 gataaaacta tcaaagaatt agcagatatt ctaggtgttt caaaagataa agtgaagtat
      901 caactgagga aattacctag taattttacc tacaaaaaag gaaatattat ctacctgaaa
      961 aaagaagcag taacaacaat tacaaaggtt ttatcaggta atgagccggg taacttacct
     1021 agtgaattac ctgattcatt acctaccgat tacctactca aggagattga aactaagaat
     1081 aatcatattg aagaaaaaga taggcaaatt gaaaaacttc aactagcttt agaaacacaa
     1141 caaaaattac tcgaccagca acaacaactc accctgcaag ccaaccaaca aatcgagaaa
     1201 ctgcaggaac aactacagtt gacttatacg gaagaatctt cggaaaataa atcaacgact
     1261 ctttctgaag aaacagaatc gatagaaaaa caatcaaaga cggagaaaaa atggtggcaa
     1321 ttctggcaat aaaaccagca atttaaatta ataaaaattg ctggttttat tcttcttttt
     1381 gcttttgcga gcagagcaac atgggcccat gttgcgatat tttggtggcg ccccaatccc
     1441 cgttttttcg ttgaatctta ctcggactgc actcgtttta cagctgattt ttctgaattt
     1501 tgaaagagca taaggaaacg tggtatcact ttcttacgaa aggtgctaga taagctgtgc
     1561 ctatcacgtt tctcagaaaa aaatttgcta tcactttttt gccattgtgg ctaggctatg
     1621 tatagtgttt tggcgaaaaa tagctataca aatactatat ttgtatgata tacataaatt
     1681 atatactgat acgaaaggaa aatttccatg gacattttag ttcgaaatat caatcccaaa
     1741 attattacaa gaatagataa acgttgtcag gaactaacaa ccaaaacggg caaaaaatgg
     1801 agtcgaaatc aatacctaaa aattttaatc gaaaacgatt ttgaccacga acttatgaat
     1861 tacaaaaaag atcaattcga tttattattc gaaaaattta ttgccattca agcagaaaat
     1921 acgaacatac taaatgacta tgttcacaca aacaaccaac tcattgaaac acttatcgga
     1981 ggaatataac atgagaaaaa cttctgttca aaaacacgta accctttatc ccagtcacgt
     2041 tgacgtttta gattccatca tcgaaaaaaa tccagacgtc aacaattatt cagaagccat
     2101 tcgcttttta tgtatgagtt atgaacctgc taccaataca accacaacca gtaaccaagc
     2161 aaaactcaat gccatgggaa aagaaatttc tatcatgaca gaaatcatgg cagaatttgc
     2221 tgatttatat ctaaaagata ctggtgttct tatcggtaca aattctaaag tatatcaaga
     2281 agtgaaagaa aaaattgaaa atcaactcaa tccttcccga cgaaatcaac ccaaaaatcg
     2341 tcaaaaagaa accaatcaaa cctttacttc tggaaatttt gacccaacaa actttctaaa
     2401 aagataaaaa aagaaaccga aaaataaaaa aattcctcga tttcttttta gggatcggtc
     2461 ttccagcaag atttgcaagt tggtaccaac ttgcgaaaaa aaaaagaaaa atccgcttct
     2521 gcgaattctt cttttttcat tttggggcgc tgccctaatc cctaagtccc ggtt
//
LOCUS       DAZKBH010000188         2544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000188 DAZKBH010000000
VERSION     DAZKBH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.194"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..535
                     /locus_tag="ACG8OO_002810"
     CDS             <1..535
                     /locus_tag="ACG8OO_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFP9046634.1"
                     /translation="QVFHPVVTELENIEKQILALINSMEQQSQPIICILPNSDAGSEI
                     IRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPVINLGTR
                     QNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQ
                     ILNDGEDIKWIQKQIAY"
     gene            508..1185
                     /locus_tag="ACG8OO_002811"
     CDS             508..1185
                     /locus_tag="ACG8OO_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9046635.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1136..2317
                     /locus_tag="ACG8OO_002812"
     CDS             1136..2317
                     /locus_tag="ACG8OO_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP9046636.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2330..>2544
                     /locus_tag="ACG8OO_002813"
     CDS             2330..>2544
                     /locus_tag="ACG8OO_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9046637.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSP"
BASE COUNT          945 a          336 c          501 g          762 t
ORIGIN      
        1 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
       61 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
      121 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
      181 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
      241 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
      301 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
      361 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
      421 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
      481 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
      541 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
      601 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
      661 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
      721 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
      781 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
      841 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
      901 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
      961 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
     1021 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
     1081 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
     1141 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
     1201 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
     1261 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
     1321 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
     1381 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
     1441 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
     1501 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
     1561 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
     1621 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
     1681 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
     1741 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
     1801 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
     1861 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
     1921 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
     1981 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
     2041 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
     2101 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
     2161 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
     2221 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
     2281 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
     2341 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
     2401 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
     2461 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
     2521 agtttttgtt tgtacatccc ctat
//
LOCUS       DAZKBH010000189         2492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000189 DAZKBH010000000
VERSION     DAZKBH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.195"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(362..583)
                     /locus_tag="ACG8OO_002814"
     CDS             complement(362..583)
                     /locus_tag="ACG8OO_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046638.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(745..817)
                     /locus_tag="ACG8OO_002815"
     tRNA            complement(745..817)
                     /locus_tag="ACG8OO_002815"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(782..784),aa:Lys,seq:ctt)
     gene            893..1123
                     /locus_tag="ACG8OO_002816"
     CDS             893..1123
                     /locus_tag="ACG8OO_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9046639.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            1167..2336
                     /locus_tag="ACG8OO_002817"
     CDS             1167..2336
                     /locus_tag="ACG8OO_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9046640.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
BASE COUNT          953 a          326 c          410 g          803 t
ORIGIN      
        1 aattgataga agcgatctta aaatttaaaa ttaactaact aaaaagtccc ctttccactt
       61 attctgttta aaagaatctt ctaatagtta aaggattagt agattctttt aaacagaaaa
      121 cctattgatc attgaaattt aaatacattc tatcagtacg ttaacacttc aaggagcata
      181 ttttagttgc gccatgattt cttactgtaa agaaggagcg ataaacattc taaaataatt
      241 tttatagtca ataaaaagcg atgatttgaa gttgggataa tgatacttcc gtaaggctcg
      301 ccgtaaactt ggagagaggt gaaattccta tgtgcttttg acaaaagcag ctgattaaga
      361 attatttcca tatgttgatt tcaacatttt ttctattatt tttaagacca aattcagtga
      421 ggcaaatagc atcatgtaaa tttagttttt ctattgaacg ttctccactt tttagttttt
      481 ttatagtact caaatttaat cctgtatgtt tagagatctc atttgcagta tggttttgta
      541 aagtttctcg gattattgtt atattagcaa tttcattacg cataattatg ttcctttctt
      601 atcaaaaata tcaatgacta cagtgggctt ttagtgaaaa aaaggggtac tacagtctct
      661 tttgaaaaca aaaaaataca gtatatacgt tttctaaatg ctaagcacca acgtttacac
      721 tgtatttaag ggtttggcac ccaatgggct gtgaggggct cgaacccgcg acccgatgat
      781 taagagtcat ctgctctacc aactgagcta acagcccaaa ttaatacttt gttattctaa
      841 cacctaaaat aaaaaagtgc aatacttatt atcagaattg agagtgaatt ttatggattt
      901 tcaagattat gaaaaaatat ttgaaagtta tccatcgata ttgaaaatta ctgatttaac
      961 taaaatgtta aatattggaa aaaataaagc ttataatttg ctgaaagata gatcaataga
     1021 aaatataaaa attggagcaa ggtacaaaat acctaaaaaa gctgtcattg agtatatatt
     1081 gaaaatacaa gatgaaggta aaaatcatgc tataataaaa tagtcaaaat ttttgccgat
     1141 atttcagttt aagaggagag attgaattga aattaagagc aaacataatt gaaaaaagag
     1201 gaacattaca agtagttgta tcttataaag actctaacgg aaaaattaga caaaagtgga
     1261 aagatacaaa attaaaaaag aaaggaaata aaaaacgagc agaaaaaatt agagatgaat
     1321 ttttactaga attggaaagt caattagcta tggaatgtga atgtgctaat ccaaatttat
     1381 tgttttatga ctatttgctt aattatttgg aagtatcaaa aaaacaagta gcatttaaca
     1441 catatgttgg gtataagcat tacgtccata atcgaattta caagtttttt tatcctaaaa
     1501 aaattaaact aaataggtta aagccttatc atttacaaga gttttatcaa tatatgttaa
     1561 atgataactg tacagcaaat acggttattc attatcacgc atttatacgc aaagctcttc
     1621 aagaggctgt tattacagaa ttgataagta caaatgtagc agataaagta agaaagccaa
     1681 aaaagaaaca atttgtttct caagtttata atcaacaaga gatcttgaaa ttacttgaca
     1741 ttttgtcaga agaaaaactc tatttggtag ttcttttaac agcattttat ggattgcgaa
     1801 gaagtgaagt tttaggatta aaatggtctg ctattgattt tttaaacaaa aaaatcatga
     1861 taaaccatgt aatcatagaa aatccagaga atatgtcaca gttaataaag aaagatcaaa
     1921 caaagaactc ttccagctat cgtacattac ctttggttga aacgattgaa cgagcattag
     1981 taaatcaagc taaatggcaa caagataatc gtattctttt aggagatgat tatattttag
     2041 aagataatga gtacgtattt actatggaag atggacggtt gatgaagcct caatatgtaa
     2101 cgcatcgttt gagtaagttg attaagaaaa atggattgaa aaaaattcgc tttcatgact
     2161 tgcgtcattc taatgcttca ataatgttag atagtggcca gaatatgaaa tccattcaag
     2221 aatggctggg acatgcatcg tattcgacaa ctgctaattt atacacgcat ttaaccgcag
     2281 gagtaaaaga tcgtgcagca gaagcattag aaaacgcatt tgggtttaaa gaataaaaaa
     2341 acaaaaaaca atatctttgg ggtatcgctt cttgttttta gaattttttt gttggcaatt
     2401 tgttggcagt atgttggcaa tgaaaaaatt cacaaactaa aaacctattt ataaagcttt
     2461 ataaaacttt tttcacaaat agagcatttg gt
//
LOCUS       DAZKBH010000190         2405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000190 DAZKBH010000000
VERSION     DAZKBH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.197"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACG8OO_002818"
     CDS             5..1150
                     /locus_tag="ACG8OO_002818"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP9046641.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACG8OO_002819"
     CDS             1321..1779
                     /locus_tag="ACG8OO_002819"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9046642.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2405
                     /locus_tag="ACG8OO_002820"
     CDS             1938..>2405
                     /locus_tag="ACG8OO_002820"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFP9046643.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          801 a          452 c          455 g          697 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctt
//
LOCUS       DAZKBH010000191         2388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000191 DAZKBH010000000
VERSION     DAZKBH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.198"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACG8OO_002821"
     CDS             197..2059
                     /locus_tag="ACG8OO_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9046644.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2388
                     /locus_tag="ACG8OO_002822"
     CDS             2059..>2388
                     /locus_tag="ACG8OO_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9046645.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          859 a          353 c          465 g          711 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAZKBH010000192         2349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000192 DAZKBH010000000
VERSION     DAZKBH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.200"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1798
                     /locus_tag="ACG8OO_002823"
     CDS             185..1798
                     /locus_tag="ACG8OO_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9046646.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          739 a          416 c          452 g          742 t
ORIGIN      
        1 atacagggtt atccccttca aagtattatc tatccttaaa atagaaaaaa atttttaggt
       61 tttatccaca aattatttct ctatattttc tgtctattca aaataaacaa taattttccg
      121 gcacaattat ttgctgtttt ttaccataac atagtacgat caacgtagaa aaggagatgt
      181 ttttatgaaa gttgttgtca ttggcggtgt agctggcggt ccttcatttg ccactcgttt
      241 ccgtcgattg aatgaagcgc acgaaatcat tatctatgaa cgcggagaaa atatttctta
      301 tgcaagttgt gctttgcctt attatttagg aggtgtgatc actgaccgtg actcgctgat
      361 cgaacgtaca ccagaaatat tgaaaacaaa aaacaacatc gatgtcttta ctaaacacga
      421 agtaacagca atcgatcctt ctactaagcg attaacagtt aaagacctat ccacaaatga
      481 agaaataaaa acagattacg ataagttgat catctcttct ggtgctagac cagattatcc
      541 ggatattccc ggagtttttg aagcagaaaa cggctttgtc ctccgtagtg tgacggatgc
      601 ggatcgaatc aaatcgttcc ttgaagaaaa aaatccacaa catgtcgtca ttcttggtgc
      661 aggtgttatg ggtctggaat tagctgagaa tttcaagcat cgcggcttag acgtgacttt
      721 aatcgatcaa ttgccacaag tcgctttccc ttatgatcca gaaattgcta atttagttta
      781 tgacaaattg ctgaaagaag gattagccgt tcatttagaa acaagagtta ctgagatccg
      841 tgataaaggt cgagaaatag tattatcaga tggctccgtc ctttctgctg atatgctaat
      901 ttttgctgtt ggtgtttctc cgaagaatga agtggtgaaa gcaaccggca tacaattatc
      961 agatacaggt cagatcattg ttgatgacca gttacaaacc aatctgccgg acatctatgc
     1021 gattggcgat attatcgaaa cgactagtgt agtgactggt cagccgatcc aaagtatgct
     1081 ttccagtgcg gccaatcgtc aaggacatat gctggcagat attttaaatg gtacgcctat
     1141 gcgctatcgc ggatatattg gtgcaggtgt cgcaaaaatc tttgatcata cagcaagtta
     1201 tgcgggaatg acagaacatg aattaaaagc atcaggcata acaaattata aaactgtttt
     1261 tatcactccc tttgaccatg cctatttcta tccagaagct acaagattaa atctaaagct
     1321 gatttttgat gcagatagcg gtcgtatttt aggtggacaa gcatttggag aaaaaggtgt
     1381 cgataaacgg atgggagaac tttctgtagc aatcaccgga aacttgacag tctttgattt
     1441 gcctgatttg gagttgcctt actctccacc gtattctact acccgtgacc cgttgaatat
     1501 agctggttat gtcgcaatca atcaaatgac gaatatcgta gaaacgatca aagcaagtga
     1561 tatacccgaa aacgatttga aagaagcgtt ctttttagat atacgtgaac ctaataaagc
     1621 accttctggc agtatctcgg caacaaaaaa tattccaatg aatgagttac gagatcgaat
     1681 caatgaaatc ccaaaagata aaaaagttta cattactttc agaagaggat tgaatactta
     1741 tacttctgcc cgaatcttgg caggtttggg tatcaaagcg attttgattg aagaataaaa
     1801 aaacgaccgc ggtgggtagc cgcggtctag gagttatttt tacttgagga gtaaaaatga
     1861 aaaagtatat agggttgttg gttggtatgt atatagaata gaagataaat gtgaagaaat
     1921 tgtgcattat ttcttcacat ttattaggtt atagtttaag aaagtttaaa cattgcgcaa
     1981 tttaattatt ttagctgttt cttttcataa aaaaacatgt tgtgactctg agacaaatca
     2041 tagtccatag tgatgtctct agagtaacta cgcctaatat tacgaggcat catattaatc
     2101 tatcagcact agtacctaaa ctagcatccc tttcgaatat tttttcttat ataataaagt
     2161 ttgctttcac tcctctaatc tcaccaaatc gactggcttg cgttgtgtga tactaggaac
     2221 tgattggttt agaacgactt ttctattgta atcatatttt tcgccatcct cgttgtcttc
     2281 taatccttca atgaatgatc cctaatgtgt gtattaaata atttaatttt tttgttccta
     2341 tttatttaa
//
LOCUS       DAZKBH010000193         2339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000193 DAZKBH010000000
VERSION     DAZKBH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.201"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..178
                     /locus_tag="ACG8OO_002824"
                     /pseudo
     CDS             1..178
                     /locus_tag="ACG8OO_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            242..>2339
                     /locus_tag="ACG8OO_002825"
     CDS             242..>2339
                     /locus_tag="ACG8OO_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9046647.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETC"
BASE COUNT          858 a          313 c          436 g          732 t
ORIGIN      
        1 caaacaaacg atgttgtatc gtgaattact ttcagatgaa gaatcaaaag aagatctgat
       61 tgcaacaatc gttgatattt tggggtatgc gatttcgcca gagtatcttt tcaacgtctt
      121 agcggatcaa gcaaaacaag caacgttcca attgaatgat ctaaacaaag cctttttagc
      181 aagtcatatt tatggaaaaa tactgtcggc aaaattcgaa tagtgtcgag gaggtgctgt
      241 gatggaagaa ttaaaagaac gcattgtcaa ttatgtcata gagaactctg agattaaaaa
      301 taaatggggg aacaatacag cacagaaaat atctgaaaaa ttaaatgtta gtcgtaatat
      361 tgtctctcaa tatttgaatg aattcgtaaa agatggagaa tttggaaaaa tcaatactcg
      421 tccagtaatc ttcttttctt taccggagaa agttccaaat caacaaattt ttagctcttc
      481 agaggaagcg gaaaaatggt atgatagctc ttttagggtt ggaatagaaa gggttatcgg
      541 aagtcaagga agtctgcgaa atgtaattgc tcaaattaaa gcagcaatct actatccgcc
      601 aaatggctta cccattttac ttcatggcga gactggaact ggaaaaagct atttagcaaa
      661 ttatgtgtat aaggatttgt tggccaataa ttatatagaa gagaaggcat ctttcatttc
      721 agttaattgt agtgaatatg ccaataatcc agaacttttc ttgtccaatt tttttgggca
      781 tgtaaaaggt gcatatacag gagccgaaga tggaagaaag ggtttagcag aattagctaa
      841 taatggagtc ttatttttag atgaaattca tgcattgagc aatgaatgtc aggagaaact
      901 ttttcagttt atggatacga ggaaatttca tcggatgggt gataatgaaa actggtacac
      961 atcaagttgc tggattatat ttgcaacatc agaaccacct gaggataaac tattatcaac
     1021 tttgttgaga agaatacctg taaagctaac acttccaaca ttagacagtc gttctcttta
     1081 tgaaagaaaa cagttaatca agatattttt taaatcagaa gaagaaagac tagcaaagaa
     1141 aatactgata tctcaacaat tattagattt tttattaaac tatcgcttta gtgaaaatgt
     1201 tggcaatttg aaaaatatca ttcaattgac ctgtgccaat gccttttatt tcttagatga
     1261 tttggaagaa ctttatattc agatgtatca tttaccacaa tgggtgaatt tcaacggcat
     1321 gaaggttatg aaaagacaga atgaattaaa atatattcaa atggatgatt tggaaaaagt
     1381 agacgaagtt tttaatatca acgaacttat agagtatctt tatcaaacgt ggaagaaaga
     1441 cactgataag cagcagcttt ttgagaaata tgatagatat tttagacaaa agaatgttga
     1501 tatccaaaat aattcagctt taacatatcc cacctatcaa gagattcgat ttagacagta
     1561 tgttaaggaa atagagagag attttcaaat agaatttgac tattattatc ttcaaataac
     1621 gtctatcttt ttgaattctt ggatttatca aatccaatta acatcggacg ataaattata
     1681 ttccttcatc aatgatatta atagttggtg tagtcgagaa gttcaattag tcaatcgttt
     1741 tattcaaata ggggatattt atttaacaaa aattcaaaaa aatgttttgg ttattttatt
     1801 taagttaatg tgtagtaaag aatttcagca aaaacgttta gcactgattt tggctcatgg
     1861 agatctaacg gcaacctgta tagcaagtac tgctaatagg atcgttgaga gatatatata
     1921 cgacgccatt gatatgccac taatgacttc aagtagtgag attgcagaca aaattaatag
     1981 atacttacaa gagtgtaccg gatatgaaga aattatttta cttgttgata tgggttcact
     2041 ggagaaaatt tatctaaatt tggaactaga cgctcaaaaa aaattcggac tactaaacaa
     2101 tgtttcaaca agaatggcta ttgatgttgc aaatcagata ttattagatt taccgcttaa
     2161 acagattctt gaacaagtaa aagaaaatca aaatgtggat tatatttatg aatcatcaaa
     2221 tattaagaag gatatgattg tctgttcttg tgcaagcgga ttaggaactt cgtttaaatt
     2281 aaaaaagata cttgaagata gctttccaag ggatactgat cttgtcatag aaacgtgtg
//
LOCUS       DAZKBH010000194         2282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000194 DAZKBH010000000
VERSION     DAZKBH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.202"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACG8OO_002826"
     CDS             complement(<1..153)
                     /locus_tag="ACG8OO_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP9046648.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="ACG8OO_002827"
     CDS             complement(475..696)
                     /locus_tag="ACG8OO_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046649.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="ACG8OO_002828"
     CDS             complement(794..1039)
                     /locus_tag="ACG8OO_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046650.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="ACG8OO_002829"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="ACG8OO_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          770 a          401 c          324 g          787 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 tt
//
LOCUS       DAZKBH010000195         2280 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000195 DAZKBH010000000
VERSION     DAZKBH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.203"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..>2280)
                     /locus_tag="ACG8OO_002830"
     CDS             complement(151..>2280)
                     /locus_tag="ACG8OO_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFP9046651.1"
                     /translation="ILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVA
                     LIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTR
                     GTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQL
                     GNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVV
                     AERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDR
                     VGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKV
                     EEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTI
                     TSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAV
                     KASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGID
                     LPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANG
                     GKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTD
                     PYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPN
                     LLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          584 a          507 c          413 g          776 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
//
LOCUS       DAZKBH010000196         2245 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000196 DAZKBH010000000
VERSION     DAZKBH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.204"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..693
                     /locus_tag="ACG8OO_002831"
     CDS             286..693
                     /locus_tag="ACG8OO_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046652.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="ACG8OO_002832"
     CDS             707..1108
                     /locus_tag="ACG8OO_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046653.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..1481
                     /locus_tag="ACG8OO_002833"
     CDS             1110..1481
                     /locus_tag="ACG8OO_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046654.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1517..1816)
                     /locus_tag="ACG8OO_002834"
     CDS             complement(1517..1816)
                     /locus_tag="ACG8OO_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9046655.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1822..2052)
                     /locus_tag="ACG8OO_002835"
     CDS             complement(1822..2052)
                     /locus_tag="ACG8OO_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046656.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          807 a          298 c          376 g          764 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aactcaccta atgaatttca agcgagtttg
     1501 tttgttattt ttttatttaa tctttgtatc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtcacgata tcaaacaaaa
     1621 agtatacacc cttcttcatt ctagttttcg cagcaggaat tttgaaatag ttcttattag
     1681 aatagtagag attgattaat aagctatctt cgattgctaa aaagaaaact tctgaattcc
     1741 atactttata aaaatctttg ataaatctat tcgtaggatc gaatttaaac cataattgtg
     1801 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
     1861 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
     1921 ttgtttattt tcggaaaaag ctaatatagc taaaattgaa acatccatct ggaatttatc
     1981 ttttttgctg ctttgctcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
     2041 gtcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct cgttgtaaag
     2101 cgatatttga ggggcaaaaa gggggcaaaa atacgtttat ctttgatata tctcagattg
     2161 aacatagtgt ttattatatg tcggtattgt tgatttatag ctgttttgag ggtattagat
     2221 acataataga atatagtggt tttgt
//
LOCUS       DAZKBH010000197         2232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000197 DAZKBH010000000
VERSION     DAZKBH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.206"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..543
                     /locus_tag="ACG8OO_002836"
     CDS             <1..543
                     /locus_tag="ACG8OO_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9046657.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(899..1471)
                     /locus_tag="ACG8OO_002837"
     CDS             complement(899..1471)
                     /locus_tag="ACG8OO_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9046658.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1487..2092)
                     /gene="fic"
                     /locus_tag="ACG8OO_002838"
     CDS             complement(1487..2092)
                     /gene="fic"
                     /locus_tag="ACG8OO_002838"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFP9046659.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          453 c          338 g          740 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
      601 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
      661 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
      721 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
      781 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
      841 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
      901 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
      961 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1021 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1081 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1141 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     1201 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     1261 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     1321 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     1381 tcttctctac gcagttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     1441 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     1501 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     1561 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     1621 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     1681 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     1741 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     1801 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     1861 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     1921 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     1981 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2041 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2101 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     2161 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     2221 ttgcacagtc ta
//
LOCUS       DAZKBH010000198         2208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000198 DAZKBH010000000
VERSION     DAZKBH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.207"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            488..913
                     /gene="bacA"
                     /locus_tag="ACG8OO_002839"
     CDS             488..913
                     /gene="bacA"
                     /locus_tag="ACG8OO_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFP9046660.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            943..1107
                     /locus_tag="ACG8OO_002840"
     CDS             943..1107
                     /locus_tag="ACG8OO_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046661.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2065..>2208
                     /locus_tag="ACG8OO_002841"
     CDS             2065..>2208
                     /locus_tag="ACG8OO_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9046662.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFE"
BASE COUNT          711 a          362 c          396 g          739 t
ORIGIN      
        1 gttattcaat agcatggaaa ctcaaagaat aagacgttcc ctggaataaa aaaaaggctg
       61 ctcgttcgag cagtccatac ataggaaaat gaaaaaacga cgtcccttac tgagaaacgc
      121 tattaaactt ttaacaaatc ctctaaaaaa taacaagtct ttaaaaagtg accggtaaaa
      181 atgcacaaag aactaaaaaa gagaaattgc atcaaatcaa gcattagaag ctagtaacta
      241 gctagaccaa ttgaaaatca caaagtgagc gcctgtatct attgctacag gcgctacttg
      301 gcatagtata cattatgtca acaagaattt aatataacat atttttctag tttgttataa
      361 caatgccact ctaacaaaaa tgtaagcatt atccttatta aaagctcctt gacaaggact
      421 atacctttta ttttatgatt tttacataga aaacgttttc tctatttttt gtttagaggt
      481 gatttttatg atttgcaaga acaagaaaaa aattattgct attattgcta gtttctcttt
      541 gatttttagt actgcatgta ctgtcttatt aacaaataat gctaatgttg aagctgcaag
      601 ctcttcttac aaacataatc atgctggtta tagatgttac aaaatgagtt attatgtaag
      661 tccttctaaa tgcaaacaat tggctcgcca aactaataaa cttaataccg tctcagctat
      721 tgcaaacttt attggactat ctggattaac accggggatt atatcattag ttttcggatc
      781 agcagttagt gctaataatg tatttgttac agccgctaga agaggaaaag gcgttcaatt
      841 aaattatatt gcccatttta gtaataccac tacctataat tatagttcta accaaaatta
      901 tgttataaaa taattaaaac tttaaactag gaggaatgct ttatgaaatt aacaaaaatt
      961 gctgtaattt tagctgttat tgctggcatt attttatatt ttgtattgcc tgctaatcaa
     1021 ttcttccttt actttggaat acctgcgatt ttaatagtaa atggcgtaag aatctttgaa
     1081 caacgaaaaa aatgtagctc tacctaatcg ttacaaaaca acaatttcac cttcggtgaa
     1141 ataagcgaaa ctttacaaaa agtggctagc aaatgctagc cttttttgta ttgtagagcg
     1201 tgaattttga gggggattca agggggagct tgcgcccctt tgacaagcgg ttatttgcac
     1261 caaatacctc cgtatttggc tagcaatcct tctaaaaaat acacctatcg atcagagtca
     1321 aaacaaatca attttcgagt gagcgaatcc gaatttgcaa agctgaagct ttgcaaatga
     1381 gtgtgccggc ttccgtaaaa atacatatgg ctataatttg cgttttaagg cgttttaaac
     1441 gttctggtat ataattatac caaaacaact ttaaaacgcc ttagatctaa ttttagaggt
     1501 cttatagaac attttagggt tgttagggct tgccctgacc gcctgtaagg cgctttgaga
     1561 acaagataag ggttttatac taagactgtc ttaaaacggc ttaaacaaac gctcagaact
     1621 tttcaaagga gttctttatg aactgttttt aagcaaagga gcctttaggc gactgcgcag
     1681 tataatgtga gagcgtagcg atcacacatt tttagtgacc gtagggagcg agtttcccag
     1741 tttagaaaaa ttcgcgaact tgtttgagga aaaaagcagg agttttttag gcgggaaatt
     1801 ttgcctcctc ttttataatc tctttttaaa cctcttttta aacctcttta ccgtcggctg
     1861 tatgcttact ctcccaaggt gtgcagaatc ttttgggggt ctttttgtct gtttttaggg
     1921 gtctttttgt ctgtttttag gggtcttttt gtctgttttt gggggtcttt ttgtctgttt
     1981 ttaggggtct ttaattaggg gcttgacccc tattagaaag ctctttataa ttttgttaag
     2041 tagttaatct tgaaggagga ttttatggca aatgaaattg tgaaatacca ccatgaatta
     2101 aatacgattc ctttacgcaa atttacgtca gtagaaatga atttattctt ctctatagta
     2161 tctagaatgc gtgacgtagg cgataaaaaa gtacaattta cattcgaa
//
LOCUS       DAZKBH010000199         2165 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000199 DAZKBH010000000
VERSION     DAZKBH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.208"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8OO_002842"
     CDS             complement(<1..914)
                     /locus_tag="ACG8OO_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFP9046663.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8OO_002843"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8OO_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9046664.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          734 a          381 c          417 g          633 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 atttt
//
LOCUS       DAZKBH010000200         2099 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000200 DAZKBH010000000
VERSION     DAZKBH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.209"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            776..1945
                     /locus_tag="ACG8OO_002844"
     CDS             776..1945
                     /locus_tag="ACG8OO_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9046665.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          768 a          294 c          312 g          725 t
ORIGIN      
        1 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
       61 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
      121 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
      181 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
      241 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
      301 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
      361 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
      421 gtactttatt taatttagta taaattaaca tactactata ttttaggttt catattatat
      481 aaattaataa tatactacca tttgttggta gtataattta aaggtaataa gttattcttt
      541 tattactatg tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac
      601 aatattaatg aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag
      661 acaaaatgat agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag
      721 tccattctgt aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc
      781 caattttact acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt
      841 tcgatgtcat ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct
      901 tgattatgaa aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta
      961 ttcacgttca ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa
     1021 tggtaagttt tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac
     1081 cactattatt cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga
     1141 aaaaatgtac gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc
     1201 tgaagatatt attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat
     1261 ggatgctact cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt
     1321 aattggcatt cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga
     1381 atctgcttat gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt
     1441 tttattagtc gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc
     1501 aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg
     1561 tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gtttatcaag caagttcaaa
     1621 agaagaagca aataatcaaa taaggtttat gatagataaa tggaaaaagc agtatccacg
     1681 agtggttaaa ttgctcatga atcctgctat attaaccttt tataatttcc ctccgtcaat
     1741 cagaagaact atttattcaa ctaatttgat tgaaggtttt aacaaacaat taaagaaata
     1801 taccaaaaga aaagagcaat ttcctaatga agaatctctg gagagatttc tagtatctca
     1861 attcaacaac tataatcaaa agtttctatg ccgagttcat aaaggtttta aagaaataca
     1921 tgatacatta gaatcaatgt tttaacttga tcaacagaat gggcttttcc atttacacaa
     1981 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
     2041 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcactt
//
LOCUS       DAZKBH010000201         2082 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000201 DAZKBH010000000
VERSION     DAZKBH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.210"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..586
                     /locus_tag="ACG8OO_002845"
                     /pseudo
     CDS             60..586
                     /locus_tag="ACG8OO_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            741..1316
                     /locus_tag="ACG8OO_002846"
     CDS             741..1316
                     /locus_tag="ACG8OO_002846"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9046666.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1359..1556)
                     /locus_tag="ACG8OO_002847"
     CDS             complement(1359..1556)
                     /locus_tag="ACG8OO_002847"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046667.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            1522..1725
                     /locus_tag="ACG8OO_002848"
                     /pseudo
     CDS             1522..1725
                     /locus_tag="ACG8OO_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1898..>2082)
                     /locus_tag="ACG8OO_002849"
     CDS             complement(1898..>2082)
                     /locus_tag="ACG8OO_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046668.1"
                     /translation="YKNLKTGAIITEKEFKNLVTEEESRFYNLSGNICPGFMDQIIEN
                     ESIESIENYVPVDLPK"
BASE COUNT          734 a          327 c          383 g          638 t
ORIGIN      
        1 gaagtccctt ctgtagaata gagttaccac aacacattca cagaaaagag gacttccata
       61 tgaacgattt tactacagaa atcctaaaga ctctagcgaa caaaggcgat ttgaatggat
      121 tattccgtgt ccatttggaa aaagctgtca atacgcttct caaaacggag ttaacggctt
      181 tcctcgatta cgaaaagtac gatcgcattg gttttaacac gggtaattct cgtaacggct
      241 cctatgaccg tacggtcaag accgagtatg gggaacttca tctccagatt ccgcgcgacc
      301 gcaatggcga gttcaagcaa cagactgttc ctgcttatag acggacgaat gacacattag
      361 aggagaccgt cattcacctc ttccgaaaag gtattaccat gtcggaaatc gcagacttga
      421 ttgagaaaat gtatgggcat cactacacgc cccaaaccat gtccaacatg actaaagtgc
      481 tgactgaaga agtaaatgca tttaaagtta gaactctaaa tgataagtat gtcgctattt
      541 tcatgattcc ttcagttttt cagatgatat accactcaaa aaacaatatg agagatgttc
      601 aggaatacca tctctaatgg cttataaatt ggttttaggg atagtagatg ttatagacat
      661 atcaaggaat aaacgagcag ctgcataaaa gcagatggaa ttaaggaata attgaattgt
      721 aaaagaaggg aataaattta atggcacaat tattttacag atatggaaca atgaatagtg
      781 gaaagagtat tgagctactg aaagtggctc ataattatga agaacaaggg aagagagtta
      841 tcataatgac aagcgcaata gatactcgat caggatttgg aaaagttgag agtcgtatag
      901 gtgtttcgaa accagctatt gcaattactt ctgaaatgga tgtgtacgga gaattagtgg
      961 agattattga tcagtacgga aaagttgatt gtattttggt cgatgagtct caatttttaa
     1021 gtagagagaa tattattgat ctatcactta tcgtaatgaa tgaggaagta ccagtaattt
     1081 gctatggatt gaagaatgat tttcaaaata atctcttcaa gggatccgaa gcattattga
     1141 catttgctga taaaattgag gaaattaaaa ctatttgcca tttttgcaat aaaaaggctg
     1201 taatgaatgc tcgtattgtt gatggtattc cagtttattc aggtgaacag attgtagtag
     1261 gaggtaatga agattacctt tcggtatgtc gcaaccacta ctacaacgaa gattgaaata
     1321 aaaaacgtgt aatcaaataa ttgattacac gttttttatt attctgttaa aggttgagga
     1381 tcagaaattt ttaggtatct ataatcctca cctaaaaatc cgttaaaacg aagtaaaaca
     1441 tcatgaccat ttaaatcagt tggaactaaa attggaatta atttatcaac agtagttttg
     1501 ttgtaaagtt caaaagtgtt ctccattcct tctggttcta caatcgaatc tttcaataaa
     1561 caaatcaaga aatacagccg cagaaaagag cagtttcaaa atgaagaatc actagagcgt
     1621 ttcctagtat ccatctttga tacatacaat caaaaattct taaatagaag ccataaaggc
     1681 ttccaacaag tgacggatac attagcttca atgtttactg agtaacccat tattttgcag
     1741 aaggacgagt tatttacaca aaattattga cgctccctgt ttggagtggt aaaaagattg
     1801 agaaattgtt tcattagcta ttacgattat tattgtaata tatttttatt gaaaatcaaa
     1861 attaaaataa ttcactgtaa aaacaggatt attttttcta ttttggtaag tcaacaggaa
     1921 catagttttc aattgattca attgactcat tttcaataat ctgatccata aatccagggc
     1981 aaatattacc agacaaatta taaaacctgc tttcttcttc agtcactaaa tttttaaatt
     2041 ctttttcagt tataatagct ccagttttca aatttttata gt
//
LOCUS       DAZKBH010000202         2065 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000202 DAZKBH010000000
VERSION     DAZKBH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.211"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1008
                     /locus_tag="ACG8OO_002850"
     CDS             <1..1008
                     /locus_tag="ACG8OO_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046669.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ACG8OO_002851"
     CDS             1025..1174
                     /locus_tag="ACG8OO_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046670.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ACG8OO_002852"
     CDS             1171..1617
                     /locus_tag="ACG8OO_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046671.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ACG8OO_002853"
     CDS             1619..1756
                     /locus_tag="ACG8OO_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP9046672.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="ACG8OO_002854"
     CDS             1794..>2065
                     /locus_tag="ACG8OO_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046673.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DAZKBH010000203         2057 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000203 DAZKBH010000000
VERSION     DAZKBH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.213"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..336)
                     /locus_tag="ACG8OO_002855"
     CDS             complement(<1..336)
                     /locus_tag="ACG8OO_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9046674.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(403..558)
                     /locus_tag="ACG8OO_002856"
     CDS             complement(403..558)
                     /locus_tag="ACG8OO_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP9046675.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1175..1396
                     /locus_tag="ACG8OO_002857"
     CDS             1175..1396
                     /locus_tag="ACG8OO_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046676.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1397..1654
                     /locus_tag="ACG8OO_002858"
     CDS             1397..1654
                     /locus_tag="ACG8OO_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046677.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1790..>2057)
                     /locus_tag="ACG8OO_002859"
     CDS             complement(1790..>2057)
                     /locus_tag="ACG8OO_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005727 - extrachromosomal circular DNA
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rep family protein"
                     /protein_id="HFP9046678.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          603 t
ORIGIN      
        1 tttagcatat tgaaattttt ctggattatc catatgagca aaatatcgca ccataccttt
       61 tgcattagca catttttgcg ggtttggaga ccgaagcttt tgagttatct cacgtatttg
      121 atcatacgtc tttttaccgc taaacattat caaaacatgc caatgaggct ttttcaattc
      181 accgtcagga ttcacatctt tatcatgaag tggactctca acccaaggaa catgtaactc
      241 atctaaaaac tccctccaat tttcaggagc cgattcagga tagactacaa acgtccaatt
      301 ccttgtgcgt tcatctttag tttttctact cgtcatgcta taattacctt aattgaacaa
      361 cagtctaaca gcaaaagcag tgcgccaaca ctaatgcttt tgctattttt tttgctcatt
      421 ttcctttccg ttagcattaa cccaaattgt aataagacca gacttactaa gacccatttg
      481 tttcgcaatt ttatcaagat tatctgccac ttccttagta agcgttacaa cgactctctt
      541 tttttcatta ctgatcataa ttcgtatcct ccaacgactt tttcttaaaa accatagtaa
      601 caaaaaagta actaaggtgc aacagtttag atactaatta gcgaataata ggccttaaac
      661 ccttggcatt actgattttg tcaaaaaaaa acgctgaaac ccttgggaga gtaaggctaa
      721 acagctatcg accaatgacc aatgaccaat aaggggggtg tcgtagtccc cccctcctcc
      781 gcccaattca taaacaccaa aaccaaaaaa caaacctcag ggctccgccc cgaaccccga
      841 cctccttcta ccgaaatata tgcaggcgcc acgcttacta tttcgttcag ctatcggctt
      901 ttgcaatcta acacgaccgt ttcgctacca gtcgccttac aggctccatt ctcgcttctc
      961 ttacgtgttt taacgattgc gccgataact aaacgaaata gacgctaaat cgcttaaaac
     1021 gcaaaataga ccctttttag agcattgatt ttttctacta gtcatttcga taatataaaa
     1081 ttgaccacaa cacagaaaac ttatattata agcttatgtc gtaagtccga cgtactcacg
     1141 taatatagac ggacgttaaa tggaaggagc ataaatgaga gtgataaaat cagaaaaatt
     1201 cactttggcc gaaattatga cgaccagcga acttgcacaa gaacttgatt acaaccaagc
     1261 ttacgttttg cgcttagcga aagaacatct gactgaagga aaagaatatc gctctgccgg
     1321 tagaagaaac tatcttttca gcccagaagc ccttaaaaaa ctagaaaaag tcataaccac
     1381 caaagaaggt gagtaaatgc ctaaagaaac aactatcaac gttgaacaac tagaaaaatt
     1441 gctagaatca tattccaatg attttatcga attgaccggc ggaaaagtcg aagaaaccac
     1501 cgacctcttt gacctacttc tcacaaatat tctggaatta aactctgaag aacaaaaaga
     1561 gcataaggga aaactgatag caccttccta caggaaggtg gctaagtcgg ctgtgccaac
     1621 tggttttctt acaaaacaac cttatatttt ttgatatcac aaaaaaatcc attttggacc
     1681 tattttcggt cataatatag tacctacttt tggtcatagt ttcgtccgta gttttggtca
     1741 tagtgactaa gactttcacg cacaaaaaaa gagctgaaat taacagctct tatcctttat
     1801 ttatttttga atttacactt ccaccatgac gattcgattt tatatattgc ccgataatat
     1861 aagctgaatt atcacaaaga agcggaaacc aatcatcaaa tctttctgac attgcatagt
     1921 caattaaatc tttgatttca gtgatattct tagaatcaac aaacgacatc atctctctaa
     1981 tcaactcata tcgttctgca ctcgttactg acaaataact agcaatttct gcaccaccat
     2041 gagcgataat atcactt
//
LOCUS       DAZKBH010000204         2005 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000204 DAZKBH010000000
VERSION     DAZKBH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.214"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2005
                     /gene="acm"
                     /locus_tag="ACG8OO_002860"
     CDS             <1..>2005
                     /gene="acm"
                     /locus_tag="ACG8OO_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFP9046679.1"
                     /translation="TNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFS
                     FDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNE
                     NIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFY
                     YKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQ
                     KVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQI
                     NFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIP
                     NVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSS
                     NELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEP
                     VPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLV
                     VTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFT
                     NLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPE
                     KTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNK"
BASE COUNT          788 a          333 c          396 g          488 t
ORIGIN      
        1 tactaatata acatcactga ttcctgttca tgtctatgcg gatgcaggca gagatatcag
       61 cagtaatgtc acttcgttga ccgtggaccc aacgaatatt acagatggag gaaacataaa
      121 agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa ccaggtgatt atctttggat
      181 caattggcca agtgaaggaa atattcgcgg tgaaggattc caaaaagaaa tcccattaat
      241 gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa gattctgcgc aggttgtgtt
      301 taatgaaaat attaaaaacc tagactcagt agaaggttgg gggcaatttg agatacaggc
      361 cagaaacgta accgataccg gtgaagaaaa tacgggacca ttcaccgtga cgagcggtga
      421 taaaacagct actgtaaatg tgacaaagcc agcttctggt tcttcttcta gcgtttttta
      481 ttataaaaca ggagacatgc tacctgaaga tactaagcac attcgatggt ttttgaatat
      541 taacaataac ggcacttatg ttgaacaacc agttaaaatc agtgatgaaa tccaaagtgg
      601 acaaagatta gatccaagta cgtttgaaat taatcaaata cacttaggtg aacagaaggt
      661 ttatagagga gaagagggaa tccaacaatt tttacaagat ttccccagcg ctacatttaa
      721 tttttcagta actgataatt acattgaaat aactatacca aaaaactttg tcaatcttag
      781 gaaaataatg gtgagttata agacgatcat agaaaaccca gaacaaataa attttgagaa
      841 tcattcggaa gcttggttta aagaatttaa taaacctgct gttgatggag aaagtttcaa
      901 tcatacagtt aaaaatattt cagcttctgg tggagtcaac ggtacagtta gaggcgaatt
      961 gaagattttc aaatatatca atgacacaga gattggtatc ccaaatgtga ctttcgaatt
     1021 aagacgagca gatgaacagc ctattcaagg acagtctagt atcctgttga cttcgaatga
     1081 acagggagaa gctagtatca aaggcctgca agtaggagac tatgttgtaa aagaaaaaga
     1141 agcacccaat tggattgatt ttgatccact aagttcaaat gaactcaaat tctccataaa
     1201 cgaaaatgat acagagggag tcagtttacc tatttataac aagaaaaagg ttacgaacat
     1261 cacagcaact aaaatttgga acggtggaac cactccaaga ccgagtattt attttaaatt
     1321 attcagagca agtacaaaca attgggaacc agttcctgat gcagaaacaa aacgattaga
     1381 caatggaata acttcagtga cttgggaaga tatccaacag tatgatgata gtggaaacga
     1441 atatacgttt aaagtgcaag aagtagatca aaacggaaat gattatgtcc cttcaggtta
     1501 tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat aaagaagtca tttcagtctc
     1561 aggacaaaaa acatgggaag acaatgagaa ccaagacggc aaacgaccga cgacgatcac
     1621 agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa gaagtaagtg aaaaagatga
     1681 ttggcgctat cgtttcacaa atctgccaaa atacagagac gggcaagaaa tcatctatac
     1741 tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt gacgggtata acatcaaaaa
     1801 cagttataca ccggaaaaaa caaatatatc tataccaggt agccatataa caccgtggaa
     1861 aaattttcca gagaagacag agagagagaa aattagaaat ctattctctc aaaaattgca
     1921 aatatttgct catacaggag aatccagcga acaaagaagc aattatatta gtaagagagc
     1981 tgtacctaaa cagataacaa ataaa
//
LOCUS       DAZKBH010000205         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000205 DAZKBH010000000
VERSION     DAZKBH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.215"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8OO_002861"
     CDS             <1..378
                     /locus_tag="ACG8OO_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9046680.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8OO_002862"
     CDS             426..1826
                     /locus_tag="ACG8OO_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP9046681.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKBH010000206         2000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000206 DAZKBH010000000
VERSION     DAZKBH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.216"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8OO_002863"
     CDS             200..742
                     /locus_tag="ACG8OO_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046682.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="ACG8OO_002864"
     CDS             898..1347
                     /locus_tag="ACG8OO_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFP9046683.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2000)
                     /locus_tag="ACG8OO_002865"
     CDS             complement(1377..>2000)
                     /locus_tag="ACG8OO_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFP9046684.1"
                     /translation="STYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYV
                     RVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKE
                     FDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLEL
                     FDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          727 a          310 c          380 g          583 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga
//
LOCUS       DAZKBH010000207         1993 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000207 DAZKBH010000000
VERSION     DAZKBH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.217"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG8OO_002866"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG8OO_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9046685.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG8OO_002867"
     CDS             852..1040
                     /locus_tag="ACG8OO_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9046686.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG8OO_002868"
     CDS             1053..1610
                     /locus_tag="ACG8OO_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9046687.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG8OO_002869"
     CDS             1642..1884
                     /locus_tag="ACG8OO_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9046688.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          555 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ctt
//
LOCUS       DAZKBH010000208         1978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000208 DAZKBH010000000
VERSION     DAZKBH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.218"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..985)
                     /locus_tag="ACG8OO_002870"
     CDS             complement(<1..985)
                     /locus_tag="ACG8OO_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFP9046689.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACG8OO_002871"
     CDS             1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACG8OO_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP9046690.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..>1978
                     /locus_tag="ACG8OO_002872"
     CDS             1810..>1978
                     /locus_tag="ACG8OO_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HFP9046691.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENK"
BASE COUNT          598 a          388 c          397 g          595 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaat
//
LOCUS       DAZKBH010000209         1962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000209 DAZKBH010000000
VERSION     DAZKBH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.219"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACG8OO_002873"
     CDS             complement(<1..272)
                     /locus_tag="ACG8OO_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046692.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="ACG8OO_002874"
     CDS             complement(309..446)
                     /locus_tag="ACG8OO_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP9046693.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="ACG8OO_002875"
     CDS             complement(448..894)
                     /locus_tag="ACG8OO_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046694.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="ACG8OO_002876"
     CDS             complement(891..1040)
                     /locus_tag="ACG8OO_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046695.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>1962)
                     /locus_tag="ACG8OO_002877"
     CDS             complement(1054..>1962)
                     /locus_tag="ACG8OO_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046696.1"
                     /translation="LLDDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELII
                     GQTYTITLKGTKPASQTFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDL
                     RIFQLPKETAGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYT
                     EKGLNDTVSLTEPQSIEGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKL
                     YREGKTVTIYFFALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSAYSFVITYSI"
BASE COUNT          509 a          407 c          342 g          704 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aa
//
LOCUS       DAZKBH010000210         1953 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000210 DAZKBH010000000
VERSION     DAZKBH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.220"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG8OO_002878"
     CDS             <1..435
                     /locus_tag="ACG8OO_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFP9046697.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG8OO_002879"
     tRNA            complement(759..842)
                     /locus_tag="ACG8OO_002879"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG8OO_002880"
     CDS             1370..1870
                     /locus_tag="ACG8OO_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9046698.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          635 a          366 c          331 g          621 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cag
//
LOCUS       DAZKBH010000211         1889 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000211 DAZKBH010000000
VERSION     DAZKBH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.221"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..289)
                     /locus_tag="ACG8OO_002881"
     CDS             complement(<1..289)
                     /locus_tag="ACG8OO_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFP9046699.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFL"
     gene            complement(290..1198)
                     /locus_tag="ACG8OO_002882"
     CDS             complement(290..1198)
                     /locus_tag="ACG8OO_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFP9046700.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(1198..>1889)
                     /locus_tag="ACG8OO_002883"
     CDS             complement(1198..>1889)
                     /locus_tag="ACG8OO_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9046701.1"
                     /translation="EYYDEIIEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISY
                     QYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKL
                     SNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFS
                     WNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKY
                     IINLINDIEVN"
BASE COUNT          627 a          332 c          208 g          722 t
ORIGIN      
        1 gtaagaatga atcacgtatc acttcaattt tccctacagt atctgtagct aattcaggtt
       61 tacgttctac aattttcaca gacggattta acttagtcgt caattctact aactcattac
      121 tgtccgtatt caaacaagtc gtgcaattat gttcaggatg tttttgttga tagatatcta
      181 tcattgctaa agcgaaatgg actaggggct ttcctgccaa atatttcaaa tttttatttt
      241 taaacccctt agacccagct ctaccacaaa tagtaaataa aatttccatt tatttctcct
      301 cagctatttt caatacttcg tttgcatgat ataaatcatt ccagttttct ttgcctgtta
      361 ctacgttttc aaagaaatat ttcaactcct tctcatacat atcattagca gattcaggca
      421 tttctttaac aatgttttct tcaaaactta ttttattatt taataagtca acattgatac
      481 attcatctaa agtaaacaat tgaaattttc ttgtaggaac tcttccaaaa taatctaaat
      541 gaatttctat aattttatca tcgtatctca atatatatgt tgctaaatca ttactgttaa
      601 tttcaagatt cgaatattta ccaaactgtt tttttactga tttagggaaa ccaaataggt
      661 ttataatata atccaattca tgtatacagt ctaattctac tcctcccccc atagatttaa
      721 aggcagaata attatttcta taatcagtac cttttctcca atcaggaaga taagtagaac
      781 aaatagctct tacagaatat acttttttac tttgaatact attacgaaca acttccatga
      841 tatttttata cctcagtgga gcagcaacat aaatattttt gtaatcataa ttatattgag
      901 taagatcaat actctcagaa aaagcaggtt tttctaaaaa tatgtaatca actttttcaa
      961 caattacatt tagtgtttcc agatgcttaa aagtcggatt tgtaataaaa gctatatcat
     1021 aatgtgtatc caaatcgtct ctattttgaa tatacgttat ttcttcaaac tctttttttc
     1081 cactgctact ataagctgtt atatcaaaat taatagaatt cttttttcca tagcttatta
     1141 gattttttag atgtctcttt ccaattgacc ctaacccaaa aaaaattact ttcatcatta
     1201 atttacctca atgtcgttaa ttaagttaat aatatactta tctttttcaa aagtatattg
     1261 gggttccatt ttcttttcca aaagcgtgaa aaattcttta atctcctcta aatatgcatc
     1321 ttcaataata gtatgtgaat aattattatc atgcataacc tcatcgtaca attgaatatc
     1381 tgtcatacta gaagtttctt cattccatga aaatagagaa tccgggttac cttcccattc
     1441 tattaaaatc ttttcaccaa ttatagttaa atttctttta gctactcttg aaacaacatc
     1501 tacacttaaa gttccaacta taccagtttc atgctcaata ataagagtga aactgtccga
     1561 ataatcaata tcaagattgg ataatttctg ggtcattaca gtaaacgatt taactttccc
     1621 aaatgtatct ataatccaag gtaattcaat agcgaatatt tctctacacc cattagtttt
     1681 tttgtttgaa acaaaaaaat tgcgataatc ttcccaagga tgccagtcgg gtaaatactg
     1741 tcctacatgg tattgataag aaattttttt attatgcgtc actttagact taatatattg
     1801 aatttctttt ctcttcaaaa atgtagaaga tagataaagt aatctatctt tctttttagc
     1861 tacttcgatt atttcatcat aatattcat
//
LOCUS       DAZKBH010000212         1863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000212 DAZKBH010000000
VERSION     DAZKBH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.222"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1329
                     /locus_tag="ACG8OO_002884"
     CDS             <1..1329
                     /locus_tag="ACG8OO_002884"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFP9046702.1"
                     /translation="YAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLF
                     DDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGK
                     KAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYH
                     GQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSAN
                     WSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAE
                     GVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGK
                     NQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPID
                     MVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1319..>1863
                     /locus_tag="ACG8OO_002885"
     CDS             1319..>1863
                     /locus_tag="ACG8OO_002885"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9046703.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQR"
BASE COUNT          636 a          334 c          381 g          512 t
ORIGIN      
        1 tatgcaattg caccagagta tcttttcaac gtcttagcgg atcaagcaaa acaagcaacg
       61 ttccaattga acgatctaaa caaagccttt gtgcaattgg catctaccta caatcaattt
      121 aatgggttat ttgatgatgt ggatctccaa tcaaaaaaac taggaacaga tgagcaacaa
      181 cgaaatgtga cgattaccga agtcatcaag aaattgaatg atgtagatgt tttagaacac
      241 gatggggacg tgatcgggga tgcgtatgag tttctgatca gtcaatttgc ttcagaagca
      301 gggaagaaag ccggagaatt ttacacacct catatggttt ctgatatgat ggcgcaaatc
      361 gtaacgctcg atcaaaagga acgacgattc tttagtgttt ttgatccaac gatgggttca
      421 ggttcattga tgttaaacgt acgtaactac ttaacacacc cagataacgt taaatatcat
      481 gggcaagaac taaatacaac gacatacaat ctagcaaaaa tgaacttgat tttacatggt
      541 gtcgacgccg aagaaatgaa tctccgcaat ggggatacgt tgaataagga ctggccaaca
      601 gatgaacctt acacatttga tgcagtagta atgaacccac cttattcggc taattggtca
      661 gcagatacga ctttcttaga tgattctcgg tttaatcgtt atgggaaatt agcgccaaaa
      721 tccaaagcag attttgcgtt tcttcttcat gggttctatc atttgaagga aacaggtacc
      781 atggccatcg tcttaccaca cggagtttta ttccgtggcg ctgcagaagg ggtcatccgt
      841 caaaaattac tagaagatgg cagtatttat gctgtgatcg gtatgcctgc gaacttgttc
      901 tttgggacat cgattccaac gacggtgatt gtgttgaaga agaatcgtca aactcgagat
      961 gtattattca ttgatgctag tcgtgaattt gttaaaggga aaaatcaaaa taaactatcg
     1021 gaagaaaaca ttcaaaaaat cctcgaaacc tacgctgaaa gaaaagatgt cgagaaatac
     1081 gctcatttgg caacctttga tgaaatcaaa gaaaatgact ataacttaaa tatcccacgc
     1141 tatgtagata ctttcgaaga agaagaacca attgatatgg ttcatgtcgg aaatgacatc
     1201 aagaagatac gacaagaaca gcaagtgcta gaaaaaaaac ttttagaagc tatctcttcc
     1261 ctgcaaacaa cacctgaaaa tgaagcgtgg ttacaaggtg cgctggaggt gttcaaacat
     1321 gagcaatgat acacaaccag aaatccgttt cccaggattt acagatgatt gggaacagag
     1381 gaagttgggg gacttcgttg tagattatgt tgaaaagaca agtgtacaaa atcaatttcc
     1441 tatgctaact tcatctcaac aaaaagggat agttttgcaa gaagactatt ttgcaaatcg
     1501 gcaagtaact acagaaaata atattggtta ctttgtacta cctagaggat atttcacgtt
     1561 ccgtagtcga agtgataatg atgtgtttgt gtttaaccgt aatgacatta ttgatagagg
     1621 gattataagc tatttttatc ctgtatttac tttaaaatct gctgattccg acttcttctt
     1681 aagacgtatt aacaatggaa tccaacgaca attatctatt caagcagagg gaacaggtca
     1741 acatgttctg tctctaaaaa agtttaaaaa tatagtagca atgtttccta gtgaggaaga
     1801 acaacaaaaa atcggcacct tcttcaaaca acttgacgac acgatcgctc ttcatcagcg
     1861 taa
//
LOCUS       DAZKBH010000213         1799 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000213 DAZKBH010000000
VERSION     DAZKBH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.223"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ACG8OO_002886"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ACG8OO_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9046704.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DAZKBH010000214         1738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000214 DAZKBH010000000
VERSION     DAZKBH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.225"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1234
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8OO_002887"
                     /pseudo
     CDS             1..1234
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8OO_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1218..1610
                     /locus_tag="ACG8OO_002888"
     CDS             1218..1610
                     /locus_tag="ACG8OO_002888"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9046705.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          715 a          133 c          297 g          593 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttattgatga taatatggtt tttgatattg
     1261 ataatttaaa aggatttctt aatgatacca gttcatttgg gtttatagct aaagaaaata
     1321 ataaaattat aggatttgca tattgctata cacttttaag acctgatgga aaaacaatgt
     1381 tttatttaca ctcaatagga atgttaccta actatcaaga caaaggttat ggttcaaaat
     1441 tattatcttt tattaaggaa tattctaaag agattggttg ttctgaaatg tttttaataa
     1501 ctgataaagg taatcctaga gcttgccatg tatatgaaaa attaggtggt aaaaatgatt
     1561 ataaagatga aatagtatat gtatatgatt atgaaaaagg tgataaataa atgaatatag
     1621 ttgaaaatga aatatgtata agaactttaa tagatgatga ttttcctttg atgttaaaat
     1681 ggttaactga tgaaagagta ttagaatttt atgatggtag agataaaaaa tatacatt
//
LOCUS       DAZKBH010000215         1711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000215 DAZKBH010000000
VERSION     DAZKBH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.226"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..161)
                     /locus_tag="ACG8OO_002889"
     CDS             complement(<1..161)
                     /locus_tag="ACG8OO_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9046706.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEIS"
     gene            complement(158..811)
                     /locus_tag="ACG8OO_002890"
     CDS             complement(158..811)
                     /locus_tag="ACG8OO_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9046707.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1099..>1711
                     /locus_tag="ACG8OO_002891"
     CDS             1099..>1711
                     /locus_tag="ACG8OO_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP9046708.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          548 a          311 c          235 g          617 t
ORIGIN      
        1 ctagaaattt catttgccat attatgaatc ttttcttttc tcgtctccaa atatgtttta
       61 ggaaagataa aaaaaattaa tgaatgaact aatataatta ttattccaag aacagaaaat
      121 atttgtatga acatttttgg aaatatcttt aattttttca ttttctctcc aatttatatc
      181 ctacatttct tatagtagtt atacagtcta attgtaattt ttttctaagt tcttttatat
      241 atacatctat tactcgatca taaggagttt cttcgctatc tttccataca taatcaataa
      301 tttgcattct tgttaacact tgtccattat tatccaataa acattttaat acttctagtt
      361 cttttgcatt tacatctatt tcttcaccat ttattttagc tgtatagcta ttaaaattaa
      421 ccgatagatc cttatattca aacttctcta aatgtccata attcttctta attaaagaat
      481 ctattctagc ttttaagacc ggcaatgaaa atggcttttc tacatatccg tctactaaat
      541 tagtaaatgc atcaatttta tattcttcat cactaaatgc agtcaagatt agaattggta
      601 aattgctttt ctttctaatt tctttcaaca cttctaaacc atttataaaa ggtatctgaa
      661 tgtctaagat aaccaaattt atatcgctat taaattttga caaggcttct cttccatctt
      721 tagcttgaat aacagtatat ccaaattctg aaagatattc acttattccc tctcttatca
      781 tattatcatc ttcaacagtt aatattttca tatatacctc cacttaagcc tatacactag
      841 atattataca gcatattatg gctctataat atctagtgtt ggtgacacct tctgttgcca
      901 atactttttc ttttttctaa taaatgcaaa cagacacctt gaaatccttt agaataaagg
      961 gttctataat attttgtgtt gttggaaaat cgaaggcgat tttctactaa cactttttct
     1021 tcgtttcaac tcaaaaagag actcactttt ggtaaaattt agtcgaccaa aacatcaccc
     1081 taccaaagga gtcgctctat ggataatcat actagaaaat tactcggatt aacagataaa
     1141 catttatttt ttgatgaaga atggctgatt gaaaaagaat ataaaggcgt tcaagcgcag
     1201 tttattaaag ggagattgga tgactcacct tcccattgtg aacactgtgg atcgattcgt
     1261 attatccgca acggttccta tacgacacgt actcagatat tgaaagtcaa agaaaagttg
     1321 actattttag aattaaaacg tacccgtttt ctatgctatg attgtgggca gaccttttct
     1381 gcgaaaacgg acttagttga tgaacatcat caactaacaa aggaactgaa gcaggcgatt
     1441 ctcatagaac tgtacgaaaa tcaatcacgt aaactaatcg ctaagaagta cttcgtctcg
     1501 gatgggacgg tgacacggat cttgcgtgaa gcaacgaagc attaccagcc acgtatgaac
     1561 ttccttccga cggtgttgtg tatggatgag ttcaaatcaa tgaagtctgt ttctggttcc
     1621 atgagtttta tttgtgttga tgggacaacc catcagctat ttacgatcct tgaagatcgc
     1681 agactttaca aactaactca atacttcctg c
//
LOCUS       DAZKBH010000216         1604 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000216 DAZKBH010000000
VERSION     DAZKBH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.227"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1560
                     /locus_tag="ACG8OO_002892"
     CDS             94..1560
                     /locus_tag="ACG8OO_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFP9046709.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          446 a          292 c          333 g          533 t
ORIGIN      
        1 ttagtttata tttcttaaat aagtttttta gttaaatttt tcggtaaaga tgtataatat
       61 tttctgaaaa tttaaagaat tggggtatag attatggaaa aacagcaaga caagggcttc
      121 ttcgggcagc cgaagggatt gtcaactttg ttcttcactg agatgtggga acgatttagt
      181 tattacggta tgcgtgcatt acttatctat tatatgtacg atacagttgc caacggggga
      241 cttggattac cacgagcaac agcattagct atcatgtcga tctacggatc tttgatctac
      301 atgtctagta tcgtcggcgg atgggtatct gaccgagtgt taggagcaaa acgcactgta
      361 ttcttcggtg gagttttcat tatgcttggc cacatcgtct tggctttacc atttggtgta
      421 agtacattgt ttgtttcgat ggcattgatt attatcggaa ccgccttttt gaaaccaaat
      481 gtttctggca tggtcggaca tctttattca aagactgact tgcgaagaga tgcaggattt
      541 tcgatttttt acatgggtgt caacttaggg gcattattag caccactaat cgttggtaca
      601 atcggtcaag aatataacta tcatttaggg ttctctttag ctgctattgg gatgttcttt
      661 ggattattac aatacatcat ccaaggacgc aagacattag acggagtggg tacaacaccg
      721 ccgaatcctt tgacaactga agaacgtaaa ggattcatcc gtaacttgat catcgcactt
      781 gttgttatcg ctgcagtatt cggcggggca caactaactg gtcatctgac aattgacttt
      841 tttgtcaata caatcagtgt attggggatt atcttaccgt tgtattactt tatcaaaatg
      901 ctgacttcaa aagatgtaac tgctgaagaa aaaccaagag tatgggctta tatcccatta
      961 ttccttgcag caatcatttt ctggtcatta caagaacaag gttcatctat cttagcttta
     1021 tttgctagtg aacgtacaca atctacgcta tttggtgtaa cgatccaagc aagctggtat
     1081 caatcattga atccaatttt catctttatt ctgacaccag tattcgtaac gatgtggacg
     1141 aaacttggtg aacgtcaacc atctacagta gtgaaattct ctcttggact tttatttgca
     1201 ggattatcgt acttgctatt gatgttgccg ggtatgcttt atggtacaag tggaaaagta
     1261 agtccattgt ggttgttggg aagtttcttc atcgtggtta ttgctgaatt gtgtctttca
     1321 ccagttggtt tgtctgtgac aacaaaatta gcaccacgag catttgaatc acaaacaatc
     1381 gctatttggt tcttagctga tgcttcttca caagcaatca atgcacaaat tgcacgcttc
     1441 tacacaccgg gaacagaatc tgcttacttc ggtattatcg gtgcagtagc aatcgtcggt
     1501 ggtatcttgc tatttactgt gaaagaacca atcaaaaaac taatgggctc tattcattaa
     1561 tcaatgaaca taaaaacagg aattgcttat ttgcagcagt tcct
//
LOCUS       DAZKBH010000217         1601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000217 DAZKBH010000000
VERSION     DAZKBH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.228"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..542
                     /locus_tag="ACG8OO_002893"
     CDS             144..542
                     /locus_tag="ACG8OO_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9046710.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="ACG8OO_002894"
     CDS             526..1221
                     /locus_tag="ACG8OO_002894"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9046711.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          609 a          232 c          314 g          446 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 ttatttctca tttaaacgat gtattcccta acctctaata catccttgta actctgaagt
     1381 tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt
     1441 tcgtgtttct ctagtataac agaataagag gaacaagtcc agaagggata tttttagtct
     1501 tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg
     1561 gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa t
//
LOCUS       DAZKBH010000218         1551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000218 DAZKBH010000000
VERSION     DAZKBH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.229"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8OO_002895"
     CDS             132..344
                     /locus_tag="ACG8OO_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046712.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ACG8OO_002896"
     CDS             506..1024
                     /locus_tag="ACG8OO_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP9046713.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ACG8OO_002897"
     CDS             1031..1543
                     /locus_tag="ACG8OO_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046714.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          611 a          181 c          241 g          518 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt a
//
LOCUS       DAZKBH010000219         1551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000219 DAZKBH010000000
VERSION     DAZKBH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.230"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACG8OO_002898"
     CDS             191..445
                     /locus_tag="ACG8OO_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9046715.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACG8OO_002899"
     CDS             446..763
                     /locus_tag="ACG8OO_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9046716.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1397..>1551)
                     /locus_tag="ACG8OO_002900"
     CDS             complement(1397..>1551)
                     /locus_tag="ACG8OO_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFP9046717.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
BASE COUNT          550 a          231 c          293 g          477 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc
     1381 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
     1441 gtgctcgact taatagaaca ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc
     1501 ctttaacaga acgtgagttg aaattttgac aacctaattt ttctaaggaa g
//
LOCUS       DAZKBH010000220         1540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000220 DAZKBH010000000
VERSION     DAZKBH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.231"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1490
                     /locus_tag="ACG8OO_002901"
     CDS             <1..1490
                     /locus_tag="ACG8OO_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9046718.1"
                     /translation="QQQTIQNLTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNL
                     FSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGS
                     QMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSL
                     GSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGER
                     LRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPK
                     TFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQ
                     LDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACK
                     LRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAE
                     LNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          520 a          280 c          318 g          422 t
ORIGIN      
        1 cacaacaaca aaccattcag aatcttacaa ctgaaatcca actattgaat gaaaaggtca
       61 attatttaac caacaaatta tttggacgct ctaaagagac tctctttgag gaaacgaatg
      121 ggcaattgaa tctttttagt gatgaagaaa tttctgtaag tgttccagaa gcagctgcca
      181 ccattattcc agtaaaagga catcaacgag ttgtaggaac gaaagcagac aaaatcaaac
      241 acttacctat aacagagaaa gaacatctcc ttcctttgga agaacaattt tgtgaacatt
      301 gcggttcaca gatgaaagat atcggacgga caaaagtacg agaagaaatt cgtttccatc
      361 aggctatgtt ggattgtctc acccattatc aacataccta ttgttgtaaa agctgcgaaa
      421 aagaaggact ttcttctttt aaaaaagcga tcgttcctaa acctttgatt tctaatagct
      481 taggttccaa tagtttagtg gcggaaacga tccggatgaa atttggtcag aaagttcctg
      541 cctatcggca agagaactat tggaaacaaa cacatgggtt ggatatttcg agagataata
      601 tcaccaattg gcatattaaa gcagtgcaaa acgcgttaga ccctttagga gaacgattaa
      661 gagtctatct taatcaagaa gagattctac acggggatga aacaagttat cgtgtgattg
      721 aaagtgccaa aacagatacc tattactggc agttttgtac aggaaaagac agtcagcatc
      781 ccatcgttta ttatcaccac gacgaaagtc gtgctggaga tgttccgaaa acttttctca
      841 aggagtttac agggtattta cactgtgacg gctatagcgg gtataatgcc gtggaaagtg
      901 tacgacttgt ctattgtttc gcgcatgttc ggcggaaatt tttcgaagca attccaaaag
      961 gaaagaaaaa cacggatatt cccgcagctc aagcagtcaa acaattggat aaatggtttg
     1021 tcctagaaaa gaaatggaaa gacttttctc ctgagaagcg attaagctgt cggcaacaag
     1081 aactgcgtcc attatttatt gctttttatg agtggatggc aacgatagat cctgttgcaa
     1141 aatccaagtt ggatgcagcc gttcaatatg cgtgtaaact acgaagtggt tttgaaccta
     1201 tttttgaaga tggtcgttta gaactcacaa ataatcgtgc ggagcgaaat atcaaagagc
     1261 tggtgatagg acggaaaaat tggttgcatt ccacaagtct tgaaggagca cgaacttcag
     1321 gaatcattct gagtgtatat aaaacagcag aacttaacgg attgaatcct gtaaagtatc
     1381 tagagtttct atttgataag ataccaaatc ttcccgtact ttcagcagaa actttagatc
     1441 aactgttgcc ttggaacaag gatgtacaac aacatttttc tcgtaactaa aaacgccaaa
     1501 cattcccgat tctattctat aggtcgggtt atttgacgta
//
LOCUS       DAZKBH010000221         1509 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000221 DAZKBH010000000
VERSION     DAZKBH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.232"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..868
                     /locus_tag="ACG8OO_002902"
     CDS             101..868
                     /locus_tag="ACG8OO_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9046719.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="ACG8OO_002903"
     CDS             1060..1458
                     /locus_tag="ACG8OO_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9046720.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DAZKBH010000222         1480 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000222 DAZKBH010000000
VERSION     DAZKBH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.234"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          551 a          169 c          303 g          457 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat
//
LOCUS       DAZKBH010000223         1466 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000223 DAZKBH010000000
VERSION     DAZKBH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.235"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8OO_002904"
     CDS             131..505
                     /locus_tag="ACG8OO_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9046721.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8OO_002905"
     CDS             complement(619..1248)
                     /locus_tag="ACG8OO_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9046722.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8OO_002906"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8OO_002906"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          492 a          224 c          258 g          492 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaat
//
LOCUS       DAZKBH010000224         1460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000224 DAZKBH010000000
VERSION     DAZKBH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.236"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..421
                     /locus_tag="ACG8OO_002907"
     CDS             <1..421
                     /locus_tag="ACG8OO_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFP9046723.1"
                     /translation="DLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVK
                     RIENEVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYL
                     ILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            426..1373
                     /locus_tag="ACG8OO_002908"
     CDS             426..1373
                     /locus_tag="ACG8OO_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9046724.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          490 a          208 c          292 g          470 t
ORIGIN      
        1 agatttgact tcacctttac gtgaagtttc taatcttgaa gaattaatag atgaacatct
       61 aaaaaattta gataaagacg tctacttttc agttgtttct gcaagacgta atccctattt
      121 taatatggta aaaagaattg aaaatgaagt tactatagtt aataaatcaa gatacacatc
      181 taggcaacag acacctgaag tttttgaaat gaatgcctca atgtattctt ttaaacctga
      241 atttctaaaa aaaagtaatt atatttttga tgggcaatgt gggataataa aaatgagaga
      301 ttatctaatt ttagatattg atagtgaaga agattttaaa tggttagaat ttttgtttcc
      361 taaattttta gaagattctt cggagcttca agatatctat aattatttat atcggaggta
      421 aaaggatgag ctgttatctt atcaccggtg gggctggctt cattggttct accttagcca
      481 atcatttagg agaagaacat gtggtaatcg tcgtcgatga cttatcaatg ggaaaagtag
      541 aaaatcttga tatgacgaaa aatatcactt ttattgaggg agatgtcgct gagaaatcct
      601 tgatggaaga gatcatgaaa gctaataaat ttgattatat ctttcattta gcagcagttg
      661 ctagtgtggc agattcagtt gcccgaccag tagaaacaca tcgtgtgaat tttgaaagtg
      721 tattaatgat tttagaattg atccgcaagt atcaacctga tttaaaacgg atcgtgttta
      781 gttcttcagc ggctgtttat ggggatgaac cgactttacc aaaaaaagaa gagtccgtga
      841 ttcgcccatt gacgccttat gcggttgata aattcgctgc agaacaatat gtgctagatt
      901 attgtcattt atatgatgta cccggtagcg cagttcgttt tttcaatgtc tatggtccaa
      961 atcaaaatcc gaattctcca tacagtggtg tgatttctat tctcgtagat cgctataaga
     1021 aacaaatcgt aggagaagca acttcattta cattatttgg tgatggaagc cagtctagag
     1081 actttgttta tatagatgat gtaatccaag cattactttt agttgcgaat gaggaagctg
     1141 cactggggaa acaattcaat gttggaacgg gtaaagcaac cacacttttg gaattgattc
     1201 atgcaatcaa tgaaattctc ggtgtagaat tgacacttga atacaaagaa gagcgttcgg
     1261 gtgatatcca tgactctcta gcagatatta ctaagatcca gtcaattggc tatcaaccga
     1321 aatatggtat tttaagtgga atgactaatt atttgaaaac tgaaatcaat taaaacatac
     1381 taagataaga ttagagggta aattcaggct ctttgtcaat aaggactgat gagttttgta
     1441 aaatcaaatc tgggatagaa
//
LOCUS       DAZKBH010000225         1451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000225 DAZKBH010000000
VERSION     DAZKBH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.237"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..625)
                     /locus_tag="ACG8OO_002909"
     CDS             complement(<1..625)
                     /locus_tag="ACG8OO_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFP9046725.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ACG8OO_002910"
     CDS             complement(784..1050)
                     /locus_tag="ACG8OO_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9046726.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ACG8OO_002911"
     CDS             complement(1040..1396)
                     /locus_tag="ACG8OO_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9046727.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DAZKBH010000226         1440 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000226 DAZKBH010000000
VERSION     DAZKBH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.238"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1406
                     /locus_tag="ACG8OO_002912"
     CDS             105..1406
                     /locus_tag="ACG8OO_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9046728.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAZKBH010000227         1432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000227 DAZKBH010000000
VERSION     DAZKBH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.239"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..752
                     /locus_tag="ACG8OO_002913"
     CDS             <1..752
                     /locus_tag="ACG8OO_002913"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9046729.1"
                     /translation="FGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKY
                     TDSLEKDQEKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEY
                     RYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNA
                     IEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLS
                     SIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            767..>1432
                     /locus_tag="ACG8OO_002914"
     CDS             767..>1432
                     /locus_tag="ACG8OO_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9046730.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRS"
BASE COUNT          572 a          168 c          235 g          457 t
ORIGIN      
        1 tgtttggtgt cttaatattt ttaatcattc aaatgtttgt tgttctattt ttatttatta
       61 gaataactat taggcaacga gaacaatatg aacagcaatt gaaaagaaaa gaattggttt
      121 atctaaaaaa atatactgat tcactggaaa aagatcaaga aaaatttgcc agatttagac
      181 atgactataa aaatttattg ctgagtttaa aagaaatcgc taatcaaaac catgattcga
      241 tattggaaaa agaaataaaa gaattagaga tctattctaa ttcttatcta accaataagt
      301 ttgaatatcg ttatttaaaa aatataaaaa atgaatattt gaaaagtttg ttgatagcga
      361 agctatacca agcaaataac aataagattt gttgtaaatt cgaatgtcca agtactatta
      421 ttgaagttcc tgttccaata tttgactgta ttagggtgct tggaattatt ctcgataatg
      481 caattgaagc atctatagag agtcaagata gagaactgtc tctagctatt taccgagatg
      541 ataaacagat agaaatttcg atatcgaata gttgtagaga gatactactc tctatagata
      601 ctctaatgaa aaaaggaata acaacaaaaa agggacatca agggttaggt ttatctagta
      661 tagaggaaat caataaaaag aatcccaata tgtttgtgaa ctatcaaaac gatccgtatc
      721 aatttactac tcaagttatt ttgttgtttt gagggaagga ggatttatga gttatccaat
      781 aatattatgt gaagatcaaa ttatacaatt aaatcagtta gaacgaatta ttgataattt
      841 cattcttttt cacgataaag tatttaagat agtattgaaa acacaaagtc cgttagaagt
      901 taaaaaatac ctcaaacaat tccgaccaaa gcaaggaata tatttcttag atattgattt
      961 aaatcatgaa gttaacggta tagaattagc agaagtaatc agaaaatatg atgttcaagc
     1021 aaaaatcatt tttacaacta ctcatgatga gatgttaccc gtaacaataa aaagaagagt
     1081 tgaaacgtta ggatttgtaa caaaagatca aacactagat gagtatcgaa acgagattgt
     1141 tgagttattg ttattagcgc aagaaaggat agatgcaaca aaacaggtta cgaatcaagc
     1201 atttgttttt tccataggtt ctcaaacatt tacttttgat ataaatgaca tttattttgt
     1261 agaagcttca aaattaccac atagattatc attgtgtact aaagatgggc agtacgaatt
     1321 ttatggaaga attagtgagc ttgaaaagaa atatcctatg ttaacaagaa ttagtcgagc
     1381 gtgtttagta aatatcttta acgttaaaga aatagatttt aaaaaacgtt ca
//
LOCUS       DAZKBH010000228         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000228 DAZKBH010000000
VERSION     DAZKBH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.240"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8OO_002915"
     CDS             complement(150..1337)
                     /locus_tag="ACG8OO_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFP9046731.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKBH010000229         1416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000229 DAZKBH010000000
VERSION     DAZKBH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.241"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..760)
                     /locus_tag="ACG8OO_002916"
     CDS             complement(92..760)
                     /locus_tag="ACG8OO_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9046732.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            894..1181
                     /locus_tag="ACG8OO_002917"
     CDS             894..1181
                     /locus_tag="ACG8OO_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046733.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          528 a          224 c          235 g          429 t
ORIGIN      
        1 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
       61 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      121 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      181 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      241 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      301 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      361 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      421 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      481 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      541 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      601 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      661 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      721 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      781 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      841 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
      901 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
      961 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     1021 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     1081 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     1141 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     1201 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     1261 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     1321 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     1381 agtgacaatt tcgtatagat ttttatggta ttcgta
//
LOCUS       DAZKBH010000230         1362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000230 DAZKBH010000000
VERSION     DAZKBH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.243"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACG8OO_002918"
     CDS             82..>1362
                     /locus_tag="ACG8OO_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFP9046734.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAZKBH010000231         1355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000231 DAZKBH010000000
VERSION     DAZKBH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.244"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACG8OO_002919"
     rRNA            <1..1355
                     /locus_tag="ACG8OO_002919"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZKBH010000232         1323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000232 DAZKBH010000000
VERSION     DAZKBH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.246"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..330)
                     /locus_tag="ACG8OO_002920"
     CDS             complement(<1..330)
                     /locus_tag="ACG8OO_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046735.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYK"
     gene            complement(332..>1323)
                     /locus_tag="ACG8OO_002921"
     CDS             complement(332..>1323)
                     /locus_tag="ACG8OO_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFP9046736.1"
                     /translation="IFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSI
                     ILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSK
                     FRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESD
                     KTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLK
                     KVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKV
                     NYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          512 a          220 c          162 g          429 t
ORIGIN      
        1 tttgtaaaca ttggagaaaa gtatttttgt gaaataattt tccccctgta taaaaatttc
       61 atcataaacg gataaatcat ccgagaaaaa ttgattatgg ttagttaaca agttaatttt
      121 acgtttaaag gaagaaccga ttttccggtg attcacaaaa acaacatcat taaatagccc
      181 taattctttt atacgctcac tatatcttct agcgttttca aattcagaat aaataaataa
      241 atctcctttt gattctcggt aaaaattttg ataaacattt aatatattaa tcaaatgtaa
      301 aggagtccaa gcaatgaaag ctgtcttcat attaattctc cttttttaaa tacaagtaag
      361 aaatgcttcc caaataatac cagaaaacaa ttgaaatgac atcgcgctta ttgaaaagta
      421 catgagcctc caccatttca tctataaaaa tagatagtat aaaaattaaa acaataccaa
      481 gaaacgcata attcacttta cttctaaaaa atgttttgag taaactaaag atatttaaca
      541 catagaaagc atagatacat ataaaagcaa ttataccact tgatacaagt acttgaagaa
      601 atccattatg catgtttcca tgtgctcttt ttagttcatt cacatctgta gtatcaagat
      661 ctactttttt caaaaatttt gcatcgctat tcccatagaa atcggtctct ccataaccaa
      721 ataacaaagt tggtcttata actttaagcg cagctttcca aatcgtaaaa cgaccagctg
      781 aaatttcgga gttatcacta gaatgctgta atattacttc tttttctgga agtggagttt
      841 tatctgattc atttgactct ataacttcta cttttctatc aatcgataca ggaattgttg
      901 ctaaaaaatc cttagcataa ttagtaccaa aagaaaaata taccaataga actaataata
      961 tgcttaccat ccatttgatt tttacaggta gtacttctct aatgctcttt ctaaattttg
     1021 aattggcaaa caacaaaaga aataatacta aaaaagaaca aattactacc tgggcacctc
     1081 tagacgaaga aagtgtaata taaaatattt gtacgataaa attcatcata taaaaaacac
     1141 gaatcgtttt tgttattttt gaaattaata cactaattaa acttaaaata atagaagtat
     1201 atccaaacat agctccaaca tttggacttg tatagacacc aaataaccga tgttcaataa
     1261 aaccttgtct tgcaagtaat cctggcgttc ctgttgggac atgataagaa atcaaaaaaa
     1321 taa
//
LOCUS       DAZKBH010000233         1319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000233 DAZKBH010000000
VERSION     DAZKBH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.247"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1232
                     /locus_tag="ACG8OO_002922"
     CDS             <1..1232
                     /locus_tag="ACG8OO_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046737.1"
                     /translation="VKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLSYF
                     SYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLILA
                     YGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGETNT
                     ANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALSGA
                     ILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGARLS
                     FYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIEVG
                     CIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMIFL
                     LVPMIVSWVDNQQRISE"
BASE COUNT          428 a          130 c          234 g          527 t
ORIGIN      
        1 gtgtaaaaaa aatgctattt atggtaagta caataattgt attaacatcc cgattttggg
       61 gaattaatac tagtttatct atgcgatatt ttattatggc aatatgggat ttatatttta
      121 tgttaagcta tttttcttat cgtgagaaaa catataaaaa atacgttatc aagaataact
      181 tttggttgac tataatgccg tttgtgatat tttctatcta tactctaata atatggggaa
      241 ttggttcaaa tgcagaattt gaaaatttta taaagttatg ttccacgttg ctttatttaa
      301 ttcttgcata tggttacgca tgtacagcat tttacttatt taaggcagaa ggaattgatt
      361 ttattttttg gtcaggtgtt atttcatata tgagtggttc agttttttat ttaattgtat
      421 cttatggttt aaatggttta atttcttata taaaaagtct tataacaggt gaaacaaata
      481 ctgcaaattt tgctatggag gtacatgatt taacttttgc gatgggggtt tttttccttt
      541 attacgtatt ttttgaaaat aaaaatacaa aaaaacataa gataaaaatt atcgtatctt
      601 taattttaat tttcatggga ttgaagagaa ttgaactgtt agctttaagt ggtgcaattc
      661 tagtgtatta tgttttatta aagtggggaa aaaagattca aatccgtgca ttagtgtgta
      721 ctatttgtgc aactgttatt tcgttagtgt ttatttacat tatagataca ggaattttgg
      781 cattgcttat gtcaaatttg ggcattacag atgacggtgc gaggctttca ttttatctat
      841 attcatctaa attttatgaa cttggtttag gatatgtagg aacaggatgg acatggtttt
      901 caaaatactt ttttaatctt tatttatcgc actttaggtt aaatggtgga agaattgcag
      961 caagcctaca cagtgatata ttaactattt atatagaagt tggatgtata gtttttatta
     1021 tatggatgtt ttggttattt tttgtgaaac cgataagact aactaaaaaa tttggattga
     1081 aaacagggga gtgtgtatta ttattaacta tatatatgtt tatattatat ttgacggata
     1141 atacgctcac ttatccagac actcaaatga tttttctctt agtacctatg atagtttcgt
     1201 gggttgataa tcaacaacga atatcggaat agatagctaa agtatatttt aattatgaat
     1261 ttataattag ttgtaaaagt taatatttaa cggacaggaa gtagggtgaa aaaatttga
//
LOCUS       DAZKBH010000234         1314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000234 DAZKBH010000000
VERSION     DAZKBH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.248"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..113)
                     /locus_tag="ACG8OO_002923"
     CDS             complement(<1..113)
                     /locus_tag="ACG8OO_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9046738.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQT"
     gene            complement(409..1281)
                     /gene="scrK"
                     /locus_tag="ACG8OO_002924"
     CDS             complement(409..1281)
                     /gene="scrK"
                     /locus_tag="ACG8OO_002924"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFP9046739.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          353 a          305 c          259 g          397 t
ORIGIN      
        1 attgtttggc gtccacgttt aagctgcttc gcaacgatag caaccggagt ttcttgatga
       61 aaatatgctt ctatcattac aagctcgttt ggtgtaagat gggtataagt catttatgtt
      121 cactctcctt gtatgcttta gcgggtatta cgatttgagt gtaacataaa tggctttttt
      181 atttgtctcg cttaattata caaacggcgt gtaaaaaact attgcacaat acaagtggcc
      241 tacggctatc gaaatttcca taactttcga gcaagaattt atctcattca aggcttgatc
      301 ttccaagtaa aacaaaaacc agttaagcac tctgcctaac tggttatgac taaattttca
      361 gttgtatctt tctctaccaa cactaaatgt cgaagagcct tttttcgttt atgaatgtaa
      421 gaccgcttct cgagccaatg ccaaacaacc gagcgttcca gcgttgtctt ctaatgctgg
      481 ggtcacgata tatttgctta actcaggtgt ttcgacatac ccattgacca attttgcaaa
      541 tgcgtcgtgg acgttttgca gcatatggcg ttgtttcatc acaccaccgc caaaaatgat
      601 cacatctgga gaaaacatca aggttgtatt gtaggcacat tgagcaatat aggaggcttc
      661 gatttcccaa taaggatcat cttctgcaac ctcttgtcct tttttcccta agcgtttttc
      721 gatggcgggt ccagcggcca tcccttctaa acagttgcca tgaaatggac agctgccagc
      781 aaagtcatct tttggatgag ggacgacgag catatgcccc atttctggat gactaaaccc
      841 ttcgataaag cgcccttctt gtaaggcacc gccgccaaca cccgtaccga tcgtatagta
      901 aacgacactg ctcaatcctt tgccactgcc gaagacatac tcaccatagg cagccgcatt
      961 gacatctgtt gtccaagaaa tcgggatcgg aaattctttt ttcatggtac cgatgaaatc
     1021 aaaattttgc catgccaatt tcggggtcga ggtaatgtac ccataagtgg cggaatcgcg
     1081 atggatatcg ataggcccaa atgagccaat cccaatccct gctaaatctg cttggtattg
     1141 tttgaagaag tcaatgacta aaggcattgt ttcctcaggt gtcgttgtcg gaaagctcac
     1201 tctttccttg atcgtcattt cttcatcgcc aatggcacac acaaattttg tgccgcctgc
     1261 ttcaattgca ccgtatagca tagacatttc tccctttatt ttagaatgaa atca
//
LOCUS       DAZKBH010000235         1305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000235 DAZKBH010000000
VERSION     DAZKBH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.249"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..456)
                     /locus_tag="ACG8OO_002925"
                     /pseudo
     CDS             complement(136..456)
                     /locus_tag="ACG8OO_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(572..>1305)
                     /locus_tag="ACG8OO_002926"
     CDS             complement(572..>1305)
                     /locus_tag="ACG8OO_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046740.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDL
                     LIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT          412 a          221 c          174 g          498 t
ORIGIN      
        1 tcttatttta gcatactcag ttttagaaaa agcaaaaaaa taaagaaact tattcttgat
       61 aacaagctac gtgacgtata tccctaacac aaaaatagcc atatcctttg gtttggatac
      121 ggctatttga tattactcat tcaattatca aatcacagta ggtgtgaatc tcatcgggat
      181 cggcaatatc tacttctgat taggatcaaa ggtgattttc tcaataaagg tgtgatacaa
      241 agctttaact tgcttcttct cactctggat aagaaagcaa tccaaactag tcaatatctg
      301 ttggacatct ttgacttgaa tcggttcccc ttgttgttga aagatcccta agatttcatt
      361 ctctcgaatt tgtaactgtc ttcgtttttc agtcaattca tttaaacgat cttttaacat
      421 cggaaacaat tcgggactat cttccaaagc agcttcctgt ttttgctcta atagttagat
      481 tttgcgtacc attttcttct tttattttcc ttcgattgct gattttaata tagtctaaag
      541 gaaatttata tttattgtat tctttataac ttcaagagaa cttcgtttga gtattactgt
      601 atttttttaa ttccataaga aaattagtta ttacctgcca cctaatcagc aaatctgaac
      661 ctgagtcaat ataattaata ttattttcca tctccaacat tgtaagtact gttggtgtta
      721 atatagggac tttgtacaat tgtatttttt tgattttatc atctgcacaa aggagatttt
      781 ttaaatctaa tccttctttt atttcaaaag ttgaattttt taaatctata agagtaaaac
      841 caattccttc atactgaatt tctgaaaatc gaatttttct cccagttgat agatcaaggg
      901 gcagaaaacc ttttattaca ttgtgagtgt caccatcatt tacaatgtca ttaggaattt
      961 tgattttaga attctgtgga gcacctattg gaagataata attcattata attttgtagt
     1021 cttcatgagg aaataaagta cttttaggat tgctaagata ttctcgtacc gaatctaaga
     1081 atggcttatc cattgaatct tcatccccat ttaaactaaa aaacattcct acaattgctt
     1141 ttgcaactct aagtgaattc attttcttta ttgaatgtgt taaatcagac ttaccattct
     1201 ttaaacttgt attgttataa aggaaatttt tattgtcttc tgcaaatcta atatattcct
     1261 caacatactc actaccaaaa aaactattac atttactaca taacg
//
LOCUS       DAZKBH010000236         1287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000236 DAZKBH010000000
VERSION     DAZKBH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.250"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACG8OO_002927"
     CDS             <1..848
                     /locus_tag="ACG8OO_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9046741.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DAZKBH010000237         1263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000237 DAZKBH010000000
VERSION     DAZKBH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.251"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..1097
                     /locus_tag="ACG8OO_002928"
     CDS             18..1097
                     /locus_tag="ACG8OO_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9046742.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          375 a          301 c          310 g          277 t
ORIGIN      
        1 gacaaggatg aattcacatg acgcaaaccc atgataacac agtttcacgc aagggaaaac
       61 atctttccta ctcagaacgt tgccaaatcg ctatcctgaa aaaggaaggc tattccaacc
      121 gtcaggttgc caaagtgctg ggacgtgttc cgcaaaccat aaacaatgag attgatcgcg
      181 gaaccatcac ccagttaaaa cgccaaacgc aaaatggaaa agtgtatgac acctactatt
      241 ctgtttatga cgctgatgca ggacaggcct tctatgaaaa gcaacgactg aactgtggca
      301 gacgccccaa atgggcgaac acaaatgcct tcattgattg ggcagatgac aaaatgttaa
      361 atgaaaaatg gtcgccggac gtcgtggtcg gatttgccag aaaacacgag ctgttcgatt
      421 gtgccatcat tcctggcaca accgctcttt atcaatggat tgaccgcgga atcatgcgaa
      481 caaagaacat ggacttgctt gaaaaactgt cccgtaaaat caaagcggtc cgcccaggac
      541 accgtcccaa caagcgtgtt ctcgggacgt caattgaaga acgccctaaa gaagttgaaa
      601 cacgggaaac gttcggtcat tgggaaatcg acacggtagt gggaaacaag accaagtcgg
      661 atgccgtgct gctgacgctg gctgaacggc agacacgctt tgaagtcatc ctaaagttga
      721 acgggaagga caccgaatcg gtggaccaag cggttcaaca gctaagagaa cgggctggcg
      781 ctaacttcga tcaattattt aagaccgtta catccgacaa tggctcggag ttttctgggc
      841 tacacgatgc tctgcaagat gtagctgacg tctatttcag ccacccctat gcttcttggg
      901 agcgtgggac gagcgaaaac cagcacaaat taattcgccg tttcgtcccc aagggaagcc
      961 cgattagtga ggtgagcgag aaccagattt tgcggatcca gcgttggatg aacgattacc
     1021 cgcggaaaat tttgggttat gcgacaccac atgatacatt cgtccaggca ttcaagcaag
     1081 aacggttggt ggcttgaccc attattcctt gataacgatc ctcactcggg acatggcttc
     1141 atgtctggaa agctggcctg gggccagaat cgtctgaaga ctatcatacc tcagttcatt
     1201 atcaaggcct cactgattta gaataacgag gcagttacgg tatcaaacaa atgagttacc
     1261 tgt
//
LOCUS       DAZKBH010000238         1259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000238 DAZKBH010000000
VERSION     DAZKBH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.252"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..1097
                     /locus_tag="ACG8OO_002929"
     CDS             18..1097
                     /locus_tag="ACG8OO_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9046743.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          332 a          342 c          356 g          229 t
ORIGIN      
        1 gacaaggatg aattcacatg acgcaaaacc atgataacac agtttcacgc aagggaaaac
       61 acctaaccta ctcagaacgt tgccaaatcg ccatcctgaa aaaggaaaac tattccaacc
      121 gtcaggttgc ggatgtcctg ggctgcgtgc cgcagaccat aaacaacgaa atcaaacgcg
      181 ggaccgttac tcagcttaac cgccagaatc agaacgggaa ggtctacgac tattatcatg
      241 acgtctacga cgccgatgcc gggcaggcgg cctacgagaa gaaccgcttg aactgcggca
      301 gacggccctt atgggccgac acggacgcct tcattgaatg ggcggacgac atgatgctgt
      361 tggaaaaatg gtccccggac gtggtggtgg gctttgccag aaaacacgaa ttgttcgacc
      421 cggccattat ccctgggacc accaccctct atcagtggat cgaccgtggc atcatgaaaa
      481 cgaagaatat ggacctgcta gaaaaactgt cccgcaaacc gaaatcgtcc cgtcatggca
      541 accgcccgaa taaacgggtg ctagggaaat ccattgaaga gcgcccggag gcggttggta
      601 cccggaaaac cttcggccat tgggaagtcg acacggtcgt aggaaacaag accaagtcgg
      661 atgccgtgct gctgacgttg gtggaacggc agacacgctt cgaggtcatt ctgaagctgg
      721 ccgggaagga tgccgaatcg gtcaaccaag cggtccgaca gctgagagaa cgggctggtg
      781 cgtacttcgg ccagctgttc aagacggtta cctcggacaa cggctcggag ttcgcgggac
      841 tgcacgatgc cttgcaggat gtggcaaaca tctacttcag ccacccctat gcttcctggg
      901 agcgcgggac gagcgaaaac caacacaagc tgattcgccg tttcatcccc aagggcagcc
      961 caatcggcaa cgtgagcgaa aaccaaatct tgcggattca gcgttggatg aacgattacc
     1021 cgcgaaagat tttgggctat gcaacgccgc atgacacgtt cgtccaggca tttaggcagg
     1081 aacggttgac ggcttgacac gtgggccctt gacaacgagc ctccctcggc atggcttgtg
     1141 tctggaaagc tggccggggc cagccagtcg ggagactacc atgcctcagc tcattgtcaa
     1201 gggcccactc gattagaaaa attagacggt tggtggtaac gagcaaccgg gtcaccatg
//
LOCUS       DAZKBH010000239         1251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000239 DAZKBH010000000
VERSION     DAZKBH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.253"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..1026
                     /locus_tag="ACG8OO_002930"
     CDS             19..1026
                     /locus_tag="ACG8OO_002930"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFP9046744.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1038..>1251
                     /locus_tag="ACG8OO_002931"
     CDS             1038..>1251
                     /locus_tag="ACG8OO_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFP9046745.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEM"
BASE COUNT          492 a          162 c          240 g          357 t
ORIGIN      
        1 tcataggagg agatataagt gtttaaagga aaaacaatat tagtaactgg cggaactggt
       61 tccattggaa gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt
      121 tatagtagag gtgaagaaaa acaattttat atgcaacaag agctctcaaa tcacactaat
      181 ttgagatttt taattggtga tatcagagac tctcaaagga ttgattatgc atgtcgtgat
      241 gtggatatta tttttcatgc tgccgcaatg aaacacgttc cagcaagtga atataatcct
      301 atggaagctg taaaaacaaa tgtgatggga actcaaaatg taatagatgc agcgatcaaa
      361 aataatgtaa aacattttgt aggtatcagt acagataaag tagtgagtcc tactaacacc
      421 atgggtgcga caaagttatt gagtgaaaaa ttaataatag ctgccaatca gtataaagga
      481 aaaacagaaa caatattttc ttgtgtaagg tttggaaatg tcatggggtc aagtggatct
      541 gttattcctc tttttagtaa acaaataaaa aacaataaag atattacttt gacagatgtt
      601 gatatgacac gatttatgat gtcaataccg gatgctgcaa gattagttct gtctgctgct
      661 atgaattcaa aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa tattttaact
      721 ttagctcaat cattaataaa aatatataat gatacacatg atgaaaaata tagaggtgaa
      781 ataaaagttg taggtatcag gccaggagaa aaattatatg aagagctaat gactacagac
      841 gaaattgaac gctcatatga aaatgataaa atgtatatca ttccaccaca ctatcatgct
      901 actgacgaac tgtacaaaga ttatgaaaaa attaaaatca ctgcatactc ttcagatgca
      961 acagaattaa tgagtggaca ggaatttatt agaatcgcaa aagagaataa tgtcgtgact
     1021 tggtaaggag ttttatgatg aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc
     1081 ataatggaag tctagattta gctttagaaa gtattgataa agctaaagaa tgtggagcta
     1141 acgcggttaa atttcaaaca tttaaaacga ataagctagt ttccaaaaag gctccaatgg
     1201 caaaatatca gaaggataat cttaaagagg acagttctca atttgaaatg c
//
LOCUS       DAZKBH010000240         1239 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000240 DAZKBH010000000
VERSION     DAZKBH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.254"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..702
                     /locus_tag="ACG8OO_002932"
     CDS             <1..702
                     /locus_tag="ACG8OO_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFP9046746.1"
                     /translation="DEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTG
                     MADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSL
                     GYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGV
                     KRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVK
                     RPISADSVLLEEDVE"
     gene            699..>1239
                     /gene="neuC"
                     /locus_tag="ACG8OO_002933"
     CDS             699..>1239
                     /gene="neuC"
                     /locus_tag="ACG8OO_002933"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFP9046747.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTIL"
BASE COUNT          414 a          163 c          270 g          392 t
ORIGIN      
        1 gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa aattggctca
       61 ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt gccagtttta
      121 ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg tttcatagat
      181 agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga agatattaat
      241 ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta ctccgatcat
      301 tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt aattgagaaa
      361 cattttacct tggataagga actacctggt ccagatcata aagcttcttt agatcctcag
      421 gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg tgatggtgtt
      481 aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag cctagtggtc
      541 actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat aaaacgtcct
      601 ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt taaaagacca
      661 ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata cttatagtaa
      721 gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta ctacaagata
      781 attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat ggatttacta
      841 aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt ttgtttttag
      901 agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt ttcacaaata
      961 tatttgaaca aactagccct gattttgtat tattactagg ggatagagga gaaatgttgg
     1021 cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac ggaggcgaag
     1081 tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca tctgttcatt
     1141 ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat tcttggagag
     1201 tttttcccgt cggagctcct agaatagata ctattttaa
//
LOCUS       DAZKBH010000241         1238 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000241 DAZKBH010000000
VERSION     DAZKBH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.255"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..256
                     /locus_tag="ACG8OO_002934"
     CDS             20..256
                     /locus_tag="ACG8OO_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFP9046748.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            275..490
                     /locus_tag="ACG8OO_002935"
     CDS             275..490
                     /locus_tag="ACG8OO_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFP9046749.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT          408 a          183 c          257 g          390 t
ORIGIN      
        1 aaatattatt taaagaagcg tgtttaaaca tgcaaaatgt aaaggaacta agtattgtgg
       61 aaatgcacaa actgttggtg gaggcaataa tgcttgggga aaattggggc aagttgtagg
      121 tggacttacc acaggggcag taggtggtgc aggtttagga acagcaattt gcggaccagc
      181 ttgcggtgtt gttggagggt tatatggagc tgtcgctgga ggtgctgcag ctggctggga
      241 tgctcgaaaa aaataaaaaa ggagaataag tagtatgcat attaaaaaca caaaaacaac
      301 atttatatta tcttcagaag agttgaaaaa tattcaaggt gggagtgccg tgggagtttt
      361 aggcactact ttttctgggg caacagcagg agtaaaactt tgttcagccg gaggacctta
      421 tgcaatagct gcttgtggtg taggtggagc tttattaggt gctggattca gcatgtggac
      481 cggtgcgtaa acaattaaaa aatatcaaac ctcaagattg gtttgtgtcg attacgaaca
      541 taggtatgtt ctttatcttt tcaaaagttt ggaatggaca gaacttttta ctaaagcttc
      601 ttgtgctagt ttttataatt atattaaatt tttatctttt attaaagtca gataaaaatt
      661 ctttggaaat gtaatggtag agatattttt acaaaaggac ttgcagcgct gtagttggtg
      721 ctgcaggagg acctatttcg gccgttacag gaggtattgg taatgtactt gtcggatgtg
      781 cattaaagcc tgttcctgca agttaaattt ataaaaacag gaaagtaatt atctcaaatc
      841 ctatcaagta caatcatagg attgcacttg acaggacaaa ttttttaact aaaaaatgaa
      901 aaacactttc taaaaattag tagaatttgc caaatctttc gcatcttcca ccatacattt
      961 aattttaatt cccctttatg gtacttaata aaaacaagaa aagaactatt catttggccc
     1021 ttttttggtt ccagaccata gaaatattcc tccagtaact aatattgctg ttatgcacgt
     1081 aactaataac agctataaaa attggtacaa ctttaatagg attcagaaaa atttgcacta
     1141 gtgaaggaat ctattttatt agaaaaatgc aatctcccta aagaaaaaac cactaaacag
     1201 tgctttcttt caacttctcc ttaatttttt gtgctttc
//
LOCUS       DAZKBH010000242         1210 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000242 DAZKBH010000000
VERSION     DAZKBH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.256"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG8OO_002936"
     CDS             <1..963
                     /locus_tag="ACG8OO_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9046750.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          405 a          235 c          244 g          326 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga
//
LOCUS       DAZKBH010000243         1196 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000243 DAZKBH010000000
VERSION     DAZKBH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.257"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG8OO_002937"
     CDS             73..234
                     /locus_tag="ACG8OO_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9046751.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>1196
                     /locus_tag="ACG8OO_002938"
     CDS             253..>1196
                     /locus_tag="ACG8OO_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9046752.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERI"
BASE COUNT          494 a          105 c          209 g          388 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aattta
//
LOCUS       DAZKBH010000244         1193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000244 DAZKBH010000000
VERSION     DAZKBH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.259"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8OO_002939"
     CDS             <1..1076
                     /locus_tag="ACG8OO_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9046753.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAZKBH010000245         1178 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000245 DAZKBH010000000
VERSION     DAZKBH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.260"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..940
                     /locus_tag="ACG8OO_002940"
     CDS             155..940
                     /locus_tag="ACG8OO_002940"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFP9046754.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            933..1175
                     /locus_tag="ACG8OO_002941"
     CDS             933..1175
                     /locus_tag="ACG8OO_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046755.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT          456 a          181 c          183 g          358 t
ORIGIN      
        1 aaataatttt tgatttaatt ctgcacccaa aaagatacac tcctatctct tatttaaagt
       61 tgctatatac cctcttattt tagcatactc ccttttagaa aaagcaaaaa aaaaagaaaa
      121 aatcttattc ttgataacaa gctacgtgac gtatatgcct aacaaaaata gaacttttga
      181 atcaatctta aatgaaggtt taaccacaga accggcatcc attatttttg atgaagttat
      241 cgatgacgtt gaagatagta agtatcataa aaatttaaaa gaactttttt attttgcact
      301 caacaatgga ccaagtttgt tcactggtat atcagtacct gatagtgata atccattaat
      361 tatggcaaaa atttacttaa caaaatggtg taataaatac atcagggata ggaataaccc
      421 agcattaaaa aaacctctta aaacatttgg cgaaaaagat gctgctttaa ctactagagt
      481 agcagcaaat gctaatattg atgatcaaaa agtattgaat gactacttgc gtggacattt
      541 tttatatatg agcgctgaga acatgaacgg aagtatttta gaggaatatt tagccgaagt
      601 gttagaacct gaaggttgga tttggtgcgc cggctctgta tatcgagctg ttgatttctg
      661 ttatttagga acctcaccaa ttcttttaca agtgaaaaat aaatacaata cagaaagcag
      721 ttcgagttca gctataagag taggaacaac aattagaaaa tggaatcgtt taaataagtc
      781 gacaaaaatt tctggtttgg atagccctat ccccaattgg aaagcattaa tagaaatgac
      841 tgaagctagt aaggaactgg cagctaagtt aactgaaaat agttatttag cttatattaa
      901 tgaaaaatcc acacgagaat tatggacact cgatgactaa caatatcctt attagtatct
      961 atccacgata catggaaaaa attataaaca aacaaaaaaa ctttgaattt agaaatttct
     1021 atcttacctc agatccttat aatatctttt ggatttacga aacaatcccc accaaagctt
     1081 taaagtataa attgattgta aaaaatccaa ttactaaact taagaaaaat caacactact
     1141 ttttaggaga cgatgagttt gaacaattag tttaaaag
//
LOCUS       DAZKBH010000246         1170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000246 DAZKBH010000000
VERSION     DAZKBH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.261"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..496
                     /locus_tag="ACG8OO_002942"
     CDS             86..496
                     /locus_tag="ACG8OO_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9046756.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            510..866
                     /locus_tag="ACG8OO_002943"
     CDS             510..866
                     /locus_tag="ACG8OO_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9046757.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          412 a          165 c          219 g          374 t
ORIGIN      
        1 ttttttttat atattattag ctaataattg gtattttaaa agaaattact gtagaattgg
       61 attataatta aaaatgagtg agaaaatgat aaaagtatat acttcgccta gctgtacttc
      121 ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag agaagaatat
      181 ttttgccgaa ccgttaacag aaagtgagat caaaaagatc ttggcagcga ctgaaggagg
      241 agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca tagattttaa
      301 cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat tattgagacg
      361 acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg agatccggtc
      421 atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac aaaacaataa
      481 catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga tcagcgcact
      541 gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct tatcaatttg
      601 ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc agttttgaag
      661 aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac attgcgattg
      721 acctttcctg tgtaggaaaa aaacaactga tttttttatg cacacaacat accaatctcc
      781 tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat gagcagttga
      841 tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca ctgagcattt
      901 catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct ttttttgact
      961 ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa tctaggtggg
     1021 gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc atttctgttg
     1081 ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag ttgttgacat
     1141 gttagaaatg acatgataag ataactgagt
//
LOCUS       DAZKBH010000247         1169 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000247 DAZKBH010000000
VERSION     DAZKBH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.262"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1169
                     /locus_tag="ACG8OO_002944"
     CDS             <1..>1169
                     /locus_tag="ACG8OO_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFP9046758.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFE"
BASE COUNT          384 a          230 c          228 g          327 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgag
//
LOCUS       DAZKBH010000248         1157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000248 DAZKBH010000000
VERSION     DAZKBH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.263"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..113)
                     /locus_tag="ACG8OO_002945"
     CDS             complement(<1..113)
                     /locus_tag="ACG8OO_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFP9046759.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIK"
     gene            326..>1157
                     /locus_tag="ACG8OO_002946"
     CDS             326..>1157
                     /locus_tag="ACG8OO_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9046760.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          389 a          194 c          212 g          362 t
ORIGIN      
        1 tttttaatct tatctggtac acaattgtgg tatatgttag aaacagttct cataatttcc
       61 ttgaatgtta taaaagtatc tgtataatgt tcagtatatt ttgatgaacg catatgtagt
      121 cctcatttct tgggagtttt gagactacta tatgcgtttt tttgttgatt taccaacatg
      181 tttagacatt ttcctctagg aaaaaattaa ctagcaccac gggtttttat tacgccgatt
      241 gtaaaattaa gctagacaac taaaaaagtc atttgtgcta cactcaaaat agttccgacc
      301 aaagaattat aaggagtgag tacaaatgac ctatacccat cttacaacgg atgaacttgt
      361 aataatagag tcttatttca aaatgaatca atctgttgct aaaactgccc attgcttgaa
      421 tcgttcaaga caaacgatcc ataaagtata tctgttcttc aagcaaggaa aatcagcctt
      481 agaatattat caacagtata agaaaaacaa atcaaactgt ggtagacgtc cgcttgtttt
      541 acccgaggaa caatcagaat atattcaaag aaaggttgtt caaggatgga cacccgatgt
      601 gattgttggt cgtgcagcgt ttcctattag ttgttctgct cgtaccattt atcgtatgtt
      661 caaaaagggg ctatttgatt cttctgactt accgatgaaa ggcaagcgta aaccgaatgg
      721 acatcaagaa agacgtggaa aacaaacttt ccgccgctct attcatgaac gtgaaaagga
      781 ttatagccaa ttctcaaatg agtttggtca ccttgaaggt gacactatcg taggtctgaa
      841 acataaaagt gctgtaatta ccttagttga acgattatca aaagttatca tcacattgaa
      901 accgtgtggt agacaagcga ttgatattga aaaaaaatta aatcaatggt ttgaatctgt
      961 accgaaaaac ctattcaaat ccatcacttt tgattgtgga aaggaatttt caaattggaa
     1021 acagatcagt aatgtcaatg atattgccat ttatttcgct gatccaggaa cgccgtctca
     1081 aagaggccta aacgagaatt ctaacggatt gttacgtaga gatggtttat tgaaatctat
     1141 ggatttcaat tcagtag
//
LOCUS       DAZKBH010000249         1135 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000249 DAZKBH010000000
VERSION     DAZKBH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.264"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG8OO_002947"
     CDS             <1..969
                     /locus_tag="ACG8OO_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFP9046761.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          386 a          199 c          226 g          324 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgttt
//
LOCUS       DAZKBH010000250         1116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000250 DAZKBH010000000
VERSION     DAZKBH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.265"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="ACG8OO_002948"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="ACG8OO_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFP9046762.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DAZKBH010000251         1107 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000251 DAZKBH010000000
VERSION     DAZKBH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.266"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8OO_002949"
     CDS             <1..1087
                     /locus_tag="ACG8OO_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9046763.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          193 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaac
//
LOCUS       DAZKBH010000252         1076 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000252 DAZKBH010000000
VERSION     DAZKBH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.267"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACG8OO_002950"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACG8OO_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9046764.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          317 a          221 c          183 g          355 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaag
//
LOCUS       DAZKBH010000253         1063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000253 DAZKBH010000000
VERSION     DAZKBH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.268"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..153
                     /locus_tag="ACG8OO_002951"
     CDS             <1..153
                     /locus_tag="ACG8OO_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP9046765.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            207..575
                     /locus_tag="ACG8OO_002952"
                     /pseudo
     CDS             207..575
                     /locus_tag="ACG8OO_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            681..944
                     /locus_tag="ACG8OO_002953"
     CDS             681..944
                     /locus_tag="ACG8OO_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9046766.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1063
                     /locus_tag="ACG8OO_002954"
     CDS             944..>1063
                     /locus_tag="ACG8OO_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9046767.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPY"
BASE COUNT          407 a          132 c          215 g          309 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tat
//
LOCUS       DAZKBH010000254         1059 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000254 DAZKBH010000000
VERSION     DAZKBH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.269"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACG8OO_002955"
     CDS             complement(37..993)
                     /locus_tag="ACG8OO_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046768.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DAZKBH010000255         1024 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000255 DAZKBH010000000
VERSION     DAZKBH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.270"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..988
                     /locus_tag="ACG8OO_002956"
     CDS             179..988
                     /locus_tag="ACG8OO_002956"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9046769.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          340 a          179 c          198 g          307 t
ORIGIN      
        1 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
       61 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
      121 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
      181 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
      241 tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc atgtcagtat
      301 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
      361 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
      421 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
      481 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
      541 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
      601 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
      661 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
      721 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
      781 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
      841 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
      901 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
      961 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
     1021 gaca
//
LOCUS       DAZKBH010000256         1012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000256 DAZKBH010000000
VERSION     DAZKBH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.271"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1012
                     /locus_tag="ACG8OO_002957"
     CDS             <1..>1012
                     /locus_tag="ACG8OO_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9046770.1"
                     /translation="INNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSL
                     ATLSLIVFLLIGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFK
                     YLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHF
                     FDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSV
                     IWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKD
                     YYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPR
                     LG"
BASE COUNT          308 a          126 c          170 g          408 t
ORIGIN      
        1 tgattaataa tttcacttca tttgtatcaa ttttttcgat tcttgttggg ctaagcctaa
       61 attcggcaat taataatgca agttttgact ataaagatga cataaatgaa tttatgtcat
      121 cggtactttc tctagctaca ctttcgttaa ttgtgttctt attaataggg aacatatatt
      181 ttttgttttc aaatgctttt tttgatatga accaacttgt atttaatttg atgttattac
      241 aaagttttgc tagttttgta attagttata tccagtctta ttttacgatt aactttaacc
      301 attttaaata tttgatttta tcttttatat ctactttttt gaatattgca atctctgtag
      361 ctttgatgca gacattatat tttgatgata aatatgttgg cagagtagtc ggcggaacac
      421 tgtcatttgt gttgataggt ttagtaattt atttaagaat aatgataaag ggtgctcatt
      481 tttttgataa aaaacagtgg agatacgctt taaaaatctc tttgccgcta atcccacatt
      541 ccttagcaaa tatagtttta gcacaattcg atagaattat gattaattct tatttaggtc
      601 ctgctgcggg gggaatatat tcctacatta gtaacatagg tattattcta agcgtaatat
      661 ggatgtctac taataacgcc tgggttccat ggttctatgg tgagatgagt aaaaataatg
      721 aaaaaaaagt gaagaaagtt agtaactatt atttagtagt ctttactttg attacgatga
      781 ttgtaatggt agtttcgatt gatttagcaa aatttatggc tccgaaagac tattattctg
      841 ggttatcttt agtggttccg ataaccttag gctatttctt tcagttttta tatagtttac
      901 cagttaatgc tgaattttat gagaaaaaaa catcgtttat tgcgattggt acagtagcaa
      961 gtgctatagt taatatacta ttaaacatga ttttcatacc taggttagga at
//
LOCUS       DAZKBH010000257          995 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000257 DAZKBH010000000
VERSION     DAZKBH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.272"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..913)
                     /locus_tag="ACG8OO_002958"
     CDS             complement(26..913)
                     /locus_tag="ACG8OO_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9046771.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DAZKBH010000258          994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000258 DAZKBH010000000
VERSION     DAZKBH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.273"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="ACG8OO_002959"
     rRNA            complement(<1..>994)
                     /locus_tag="ACG8OO_002959"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAZKBH010000259          985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000259 DAZKBH010000000
VERSION     DAZKBH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.274"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACG8OO_002960"
     CDS             <1..704
                     /locus_tag="ACG8OO_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9046772.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          328 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataa
//
LOCUS       DAZKBH010000260          972 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000260 DAZKBH010000000
VERSION     DAZKBH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.275"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            339..972
                     /locus_tag="ACG8OO_002961"
                     /pseudo
     CDS             339..972
                     /locus_tag="ACG8OO_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          330 a          181 c          177 g          284 t
ORIGIN      
        1 atatttaaat ttttaccaga aagaaatgaa caccttcaat tatctaaatc tcgaaaatgg
       61 ttagatgtaa tcatcaggaa aaacaatctt aaacgaatta ctattcatgg tcttagacac
      121 acacatgcta gcttgctttt agcaagctag tgcaaatatt aaagatgtgc aagagcgttt
      181 aggccactct tctattcaaa tcattatgga tctatatatt catattacag acaaaagaaa
      241 agaagaaacc gtagcgcaat tcgcaaaata taggctctat aatttatggg actgatgaat
      301 caaaattgat tcttcagtcc cattgttatt ttaggtatat gattcgtaca ttgaaagagt
      361 tgaaaaacta tcttccgaag ccgtcaaaaa ggaccctgcc aaaagtattg atggtggatg
      421 aatttcgttc gcacgcttca attgtggata aaatgagctt tatttgtgcg gatggcgaga
      481 ctggaaaact cattgatatt ttgcctacac gaaaattgtc tcgattaaca cactatttta
      541 gtcgctgtgc aaatcctgaa gcggtggaat atttagtgac agatatgaac gccgcctact
      601 tccaactgac aaaacgtgtc ttaccaaatg cgaaagtcgt cattgattgt tttcatatca
      661 tcaaacattt gaatcaggcg ttcaatgatt ttcgtgtaca agaaatgaaa agattacacc
      721 agttagggca acgacgggaa gccgagaaag tgaaaaagaa ctggcgcttt cttctaaaaa
      781 accgtcaaaa aatcaatcac tatgattaca aaacatggcg cagttttcgt gcacctaaat
      841 acccgctact cactgaagca atgatgattg atcgattgct ggcattttca acgcctttaa
      901 aggacgccta tacttttttc catgagctga ccgaagcctt tagagacaag gaatcagact
      961 tattttttac ct
//
LOCUS       DAZKBH010000261          931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000261 DAZKBH010000000
VERSION     DAZKBH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.276"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>931
                     /locus_tag="ACG8OO_002962"
     CDS             <1..>931
                     /locus_tag="ACG8OO_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9046773.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DAZKBH010000262          922 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000262 DAZKBH010000000
VERSION     DAZKBH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.277"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..841
                     /locus_tag="ACG8OO_002963"
     CDS             <1..841
                     /locus_tag="ACG8OO_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFP9046774.1"
                     /translation="ESFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNI
                     VMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVN
                     KRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLK
                     AKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLAD
                     TISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYS
                     RDH"
BASE COUNT          353 a          106 c          176 g          287 t
ORIGIN      
        1 agaatctttt tctatagttc acaatcaggt ttattttggc gggggaatta atttatggtt
       61 agccaaaaag aatggaatca gacagagaat tgctcatagc catgcaactg aagatggaaa
      121 atctcaaaat attgtaatga atgtattgcg taaattttta accaaattgt tgttacaaaa
      181 tgcaacagac taccttgccg ttagtcaaga agctggtgaa agtctgtttc aaaaccatcc
      241 ctttgagata gttcataatg gtattgattt agagctttat agtaaaaatt cagaagctaa
      301 ggttaataag agaaaagaat tggatatctc aatgagtact tttgttgtgg gaaacattgg
      361 acgattagag aaacaaaaaa atcaatctta tttgttagag atttttttag aaatactaaa
      421 aatgaattct gattcttact tgattttagt aggtgaaggg tcacttagaa ataaactgaa
      481 agccaaagca gtagaattgg atatagagaa aagagtaaaa tttttaggag aaagaaacga
      541 tattcccgag ttattatctg tgatggatgt ttttgtaatg ccttctcttt atgaaggtct
      601 gccaatggtt ggattagaag cacaagcagc aaaattaaaa ttagtcttag cagatactat
      661 ttccaaagat acaaagttaa caaataatgt ggattttgta tcattagatg aaacaccaca
      721 agaatgggca aaaaaagtaa taaaagagcc gtatgctaat aaaataacac aagaactaaa
      781 gagttatgat gtttcgtaca ctactgaaca aatggagagt atatatagta gagatcatta
      841 aaaatgttca agatatttta ggagtgatta tttttgaaaa tatgttattt gatattggca
      901 tacaatgata ttggcaactt aa
//
LOCUS       DAZKBH010000263          908 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000263 DAZKBH010000000
VERSION     DAZKBH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.279"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..814)
                     /locus_tag="ACG8OO_002964"
     CDS             complement(<1..814)
                     /locus_tag="ACG8OO_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9046775.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIA"
BASE COUNT          312 a          108 c          153 g          335 t
ORIGIN      
        1 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
       61 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
      121 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
      181 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
      241 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
      301 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
      361 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
      421 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
      481 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
      541 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
      601 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
      661 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
      721 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
      781 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
      841 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
      901 atattttt
//
LOCUS       DAZKBH010000264          904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000264 DAZKBH010000000
VERSION     DAZKBH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.280"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..888
                     /locus_tag="ACG8OO_002965"
     CDS             265..888
                     /locus_tag="ACG8OO_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9046776.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DAZKBH010000265          866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000265 DAZKBH010000000
VERSION     DAZKBH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.281"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..>866
                     /locus_tag="ACG8OO_002966"
     CDS             21..>866
                     /locus_tag="ACG8OO_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046777.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFG"
BASE COUNT          280 a          112 c          127 g          347 t
ORIGIN      
        1 tatatgtaat ataaaaaaac atgaggtggt atatgaattt gaaaaaaatt ttttctgtaa
       61 aaatatttat agtttatgca tttgttagtt tattatattt atttccagca tttaaaaaca
      121 tggaaatatt tggcttttat gatacggcat ttcacattaa tcgtgcgtta agtttagaat
      181 ctatttttag ctcccctatt aattttgaaa cgtttagatc atatggcatg caagtaaata
      241 atttttaccc atggttaact ctatatcctt tatttttatt gatcaagttt actaatttag
      301 caataggcta taaccttttt ctttatattg ttacgctcat tactctattt atatgtcatt
      361 atgtaatgta tgaaattacc aaaaaacatg ttactagctc ctttttcgct attatatata
      421 caacatcttc ttttaggagt gtagaaatat ttcttcgagg ggcaatgggg gaattactcg
      481 caatgtcaat acttcctctt attttactcg ggtttataaa gctttacgac tcaaaaaaag
      541 aatcttgggt tatgttggcg attagcatga ctttattaat ttacactcat gttctttctg
      601 tagctatgac tatggtaatg attataattg ctttaattat tcaattttat cggaaaaaga
      661 aaatagaaat cttcttaatt attaaattaa taaaagctgc tatagttact ttacttcttt
      721 cactagcgtt tataggtcct atgattgagc agacattgta tcaagatctg aatagacctt
      781 atgtggatat attacaaaaa agagcaattt atctgggtaa agagtttttg attggaagta
      841 tagattcaga attactatca tttggt
//
LOCUS       DAZKBH010000266          858 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000266 DAZKBH010000000
VERSION     DAZKBH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.282"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            279..767
                     /locus_tag="ACG8OO_002967"
     CDS             279..767
                     /locus_tag="ACG8OO_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9046778.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
BASE COUNT          346 a           71 c          144 g          297 t
ORIGIN      
        1 tgttaaatta aaaatgaaaa aatggaagcg gttacttatt cttttgaatc taagataaaa
       61 attcaaaaga ataacataac ccaaatatga ttatacttga gattctaaat atttatcgaa
      121 aaggaatttg ttttaaggaa aaatgattct atggacggtt attgatttat atttaccaaa
      181 tggctaataa aatagtattt cttatattgt atagtttttg tttttatgtg aaaacatgag
      241 caatagaatt gtactagtag gataaggatg gatggaaaat gaaaaaaact atttttgttt
      301 tagcgatttt tatatgtttt gggctacttt tggtaggcta cttgttgttt aattctaatg
      361 aaatgagaac tatttctacg aatgacttgt taaaaaaaag tgaattaaag gataaaaaaa
      421 aagaatctct tgcctttata atgtttactc aagaagagtg tgagtattgt atagcaataa
      481 atccaatcat agatagtgta aataaggaaa ctttgaataa gataaagaaa tatgatatat
      541 ctgtagattt tcaatctaaa caaaagtgta aaattaagtt tgttccgact attgttgcat
      601 atagtaagaa taaagaagtg gggagaattg acaatgaatt agataatttg tatcgaaaaa
      661 agcaagatgg ttcaattgta atagacaaga aaaaaacaaa gacattgctt atagattgga
      721 tgaaaaaaat ggataaacaa ggagaatatt tgtatggaga agattaaaca gacatttccg
      781 ttaattaata aatattggaa taagaaaaaa atttttatat tttttattac gtttctgctg
      841 tcatctttat tttctcta
//
LOCUS       DAZKBH010000267          820 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000267 DAZKBH010000000
VERSION     DAZKBH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.283"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(274..465)
                     /locus_tag="ACG8OO_002968"
     CDS             complement(274..465)
                     /locus_tag="ACG8OO_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046779.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT          294 a          143 c          111 g          272 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatgattcc taataaagaa aagactacaa taatattttt
      601 ctaacttatc gccttcaaag aaatagtttc ttatttttca ctaaaataaa aaatttattg
      661 caaaaaaaat agaagtgaca aaattatcaa tcatttcaag gtaatataac tacctgagtt
      721 tattatcgat taaaatgcta actacatttg caagagaagc gagagttaaa ggtaaatata
      781 gaatttctac ttttttagca atagaaatag gaataaaata
//
LOCUS       DAZKBH010000268          815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000268 DAZKBH010000000
VERSION     DAZKBH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.284"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG8OO_002969"
     CDS             85..624
                     /locus_tag="ACG8OO_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046780.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          312 a          102 c          147 g          254 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcata
//
LOCUS       DAZKBH010000269          815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000269 DAZKBH010000000
VERSION     DAZKBH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.285"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACG8OO_002970"
     tRNA            8..81
                     /locus_tag="ACG8OO_002970"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Asn,seq:gtt)
     gene            121..210
                     /locus_tag="ACG8OO_002971"
     tRNA            121..210
                     /locus_tag="ACG8OO_002971"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Ser,seq:gga)
     gene            225..296
                     /locus_tag="ACG8OO_002972"
     tRNA            225..296
                     /locus_tag="ACG8OO_002972"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:258..260,aa:Glu,seq:ttc)
     gene            312..384
                     /locus_tag="ACG8OO_002973"
     tRNA            312..384
                     /locus_tag="ACG8OO_002973"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Val,seq:tac)
     gene            394..466
                     /locus_tag="ACG8OO_002974"
     tRNA            394..466
                     /locus_tag="ACG8OO_002974"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:427..429,aa:Asp,seq:gtc)
     gene            502..577
                     /locus_tag="ACG8OO_002975"
     tRNA            502..577
                     /locus_tag="ACG8OO_002975"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Phe,seq:gaa)
     gene            579..659
                     /locus_tag="ACG8OO_002976"
     tRNA            579..659
                     /locus_tag="ACG8OO_002976"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:613..615,aa:Tyr,seq:gta)
     gene            666..739
                     /locus_tag="ACG8OO_002977"
     tRNA            666..739
                     /locus_tag="ACG8OO_002977"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Trp,seq:cca)
     gene            742..814
                     /locus_tag="ACG8OO_002978"
     tRNA            742..814
                     /locus_tag="ACG8OO_002978"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:775..777,aa:His,seq:gtg)
BASE COUNT          178 a          188 c          224 g          225 t
ORIGIN      
        1 atgttattcc ggcatagctc agttggtagt agcgcatgac tgttaatcat gatgtcgtag
       61 gttcgagtcc tactgccgga gttctcattt tgagaaagag tattgagctt ctttcactct
      121 ggagagttgt ccgagaggcc gaaggagcat gattggaaat catgtagatg gtgccaaact
      181 gtctcaaggg ttcgaatccc ttactctccg tagcaaattt acctggcccg ttggtcaagc
      241 ggttaagaca ccgccctttc acggcggtaa cacgggttcg aatcccgtac gggtcatttt
      301 aattgaatgt tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
      361 gcggttcgat cccgttaacc tccataatga actggttccg tggtgtaggg gttaacatgc
      421 ctgcctgtca cgcaggagat cgcgggttcg attcccgtcg gaaccgtcgt tcttgttatt
      481 caattaataa ttttaaaata tggcgcggta gctcagttgg tagagcaacg gattgaagct
      541 ccgtgtgtcg gcagttcgat tctgtcccgc gccaccatgg aggagtagcg aagtggctaa
      601 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
      661 tttatagggg catagtttaa aggtagaaca gcggtctcca aaaccgttag tgtgggttca
      721 attcctgctg cccctgccat ggcgaatgtg gcgaagtggt taacgcaccg gattgtggct
      781 ccggcattcg tgggttcgat tcccatcatt cgccc
//
LOCUS       DAZKBH010000270          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000270 DAZKBH010000000
VERSION     DAZKBH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.286"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG8OO_002979"
     CDS             <1..672
                     /locus_tag="ACG8OO_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9046781.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKBH010000271          804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000271 DAZKBH010000000
VERSION     DAZKBH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.287"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(376..750)
                     /locus_tag="ACG8OO_002980"
     CDS             complement(376..750)
                     /locus_tag="ACG8OO_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046782.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT          225 a          174 c          112 g          293 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcaaaaact
       61 taacaacttt cgaaaacttt cgtcacaaaa tattcacaac taatgactat tatataagca
      121 taccaacaaa caaccctaac aacacttttt catttttaac tccttatcct gctagctccc
      181 cggctagcag gacctttttt tatcaaaaaa acttgtacca tccaatcgat tcaactaatc
      241 aaccgaatgc tgtcatgcta tgatactcat cctaaaacgt tccgttttac tgatatcagc
      301 ttaaagcatt cggttatttt tgattagtaa tgattgatgg tacaagctca tacgtgcttt
      361 tctatctggt ataaattatt ttgataaccg ttcctgttcc cctgctggtg aaaggattgt
      421 tattttttca atttgttcta caggaaccat ccggatctgc tgctcttcgt tttgtaggcg
      481 gatcatcact cggtcttttt cgaccgtttt tgtcgccacc cagcctgaga tcgtttcata
      541 cttgctactc aaacgatttg ctcttacttg taagataact agactatgtt cagtagcagc
      601 tttttgcagc tcaagaataa actttccttg caatcgtaca ttcttagcat gttcttcagt
      661 gtcatttaca aaagaatcaa aaagttgata cgaagtatga gtaactaact tcgtaagctt
      721 cttcattggc gatactgtct cttcgttcat cgaatgcact cctcctttag ctaagaaagt
      781 gttacttaat taattttatt ttac
//
LOCUS       DAZKBH010000272          736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000272 DAZKBH010000000
VERSION     DAZKBH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.288"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..734
                     /locus_tag="ACG8OO_002981"
     CDS             <1..734
                     /locus_tag="ACG8OO_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFP9046783.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          318 a           94 c          122 g          202 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagtt
//
LOCUS       DAZKBH010000273          733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000273 DAZKBH010000000
VERSION     DAZKBH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.289"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>733
                     /locus_tag="ACG8OO_002982"
     rRNA            <1..>733
                     /locus_tag="ACG8OO_002982"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAZKBH010000274          712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000274 DAZKBH010000000
VERSION     DAZKBH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.290"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACG8OO_002983"
     CDS             complement(<1..446)
                     /locus_tag="ACG8OO_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9046784.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DAZKBH010000275          703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000275 DAZKBH010000000
VERSION     DAZKBH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.291"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          297 a          106 c          122 g          178 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa catttttaac
      601 aagcaagcaa tatgctagca aacaaattga gcttaacaat cgcaagattg ttcgaacttt
      661 ttatgagagt ttgatcctgg ctcaggacga acgctggcgg cgt
//
LOCUS       DAZKBH010000276          685 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000276 DAZKBH010000000
VERSION     DAZKBH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.292"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>685
                     /locus_tag="ACG8OO_002984"
     CDS             <1..>685
                     /locus_tag="ACG8OO_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9046785.1"
                     /translation="ILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTRELQYNVIAK
                     LLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLV
                     DFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDI
                     CVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVR
                     RRKLIANDIL"
BASE COUNT          255 a           94 c          120 g          216 t
ORIGIN      
        1 gattttacta gtagcgtata aaattttaac tgtaaacagt aacgttgcac gtaatattgc
       61 tagtaatata agggcgattt atgtggatga atttcaagat actagagaat tacaatacaa
      121 tgtaatagct aaattacttc aaaacaaccc tcaaattcag tcaatgtttg ttggagatat
      181 tgaccaagca atttatggat cattagatgg tatagctaaa tcagttgaag aactagaaat
      241 gctaactagt cacacttttc aatcaaagac gctacatggc tgttacaggt ctaatcagag
      301 attagtagac ttctactcta attttcagag ttgtccttac gagattgaaa gcagagggaa
      361 taataaaaac gaccgtggtt ttataagtta tgattgcaaa gttactaagg aggatttacc
      421 caacataata aaaaatataa taaaggaaaa gattgaaagc ggcattcatg aaaaagatat
      481 ttgtgtactt gctcctcaac actatcctct gttttcactg ggtgagtatt taaagaaaga
      541 attaccgtat tgcaattttg atgctataaa tatatcacca attaaggtaa ataatcatag
      601 tttatttttc aagttagcaa ttttgtactt tactgaatca ggtgaaaagg ttcgaagaag
      661 aaagctaatt gcaaacgata tttta
//
LOCUS       DAZKBH010000277          663 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000277 DAZKBH010000000
VERSION     DAZKBH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.293"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..>663
                     /locus_tag="ACG8OO_002985"
     CDS             122..>663
                     /locus_tag="ACG8OO_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9046786.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMI"
BASE COUNT          230 a           94 c          108 g          231 t
ORIGIN      
        1 cgtttcaaga gatagcgaca ctatcgttat cgaaaaaggc gaaaacctat ctcattttct
       61 gctaatgtaa gttggtttag cttttgttaa tatgacttta tgaaaatgga ggtaattaaa
      121 tatgattttt cctaaagtta atgaagcaag cttgaataaa ggatcctttt ttcatatcta
      181 tgaaaacgaa gattttgaac tgtataatat cctttcacaa aaagaggtca tcgaaaacca
      241 aataatctgc catccaatta tttacaaatc tattttcaag tatgcatttt ttgcttccaa
      301 tgattttcaa agaatggaga ttaccgactg tatattcgaa aactgtgatt tttcaggctg
      361 tatattgaca aaaagcatgt tgcatcgcgt gaaatttgtc aattgtaaat taacaggtac
      421 taaattgatg gaaagttata ttgggaacac gttattcgag aattgtaaaa tggactatgt
      481 aaatttatct ggttcaaaca taaaagaaag tacctttgag cactctgtct tatcaagcgc
      541 agattttgta gactgttccc tcactaaaat aatgttttct actaatgatt tagaaaatgc
      601 tagctttttt gatactgatt taaaaaatat cgatttaagt agcaactcct ttgaaatgat
      661 tga
//
LOCUS       DAZKBH010000278          640 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000278 DAZKBH010000000
VERSION     DAZKBH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.294"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..569
                     /locus_tag="ACG8OO_002986"
     CDS             <1..569
                     /locus_tag="ACG8OO_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFP9046787.1"
                     /translation="PLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPSFDYQLFN
                     LNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGD
                     SESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITA
                     IDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          232 a           82 c          106 g          220 t
ORIGIN      
        1 cccctttaca ttggctatta gctgataaag aaaaatcaat tgtcattgaa agtatgaaag
       61 atggacttca tatatatgat aaccctgtgg gcgttcttac caataatcct tcatttgact
      121 atcaattatt taatttaaac aattatcgtg tcttatcgag tgaaactcct aaaaataatt
      181 tttcaaatca aataagtttg aatgcctata gccgcggtat gggagggata ggcttgcctg
      241 gagatttatc ctcagtatct cgttttgtta aagcgacttt tacgaagctg aattctgtat
      301 ctggagattc agagtcagaa agtattagtc aatttttcca tatcttaggt tcagtagaac
      361 aacaaaaagg tttgtgtgat gtaggtgatg gaaaatatga atatacaatt tattcttctt
      421 gttgcaatgt tgacaaagga atctattatt atcgaacata tgaagacagt caaattactg
      481 caattgatat gaataaagaa gacttagata gtcataagtt aattagttat ccaattatag
      541 aaaaacaaca aattaaatat ataaattagt taatgtgttg tgattgatta tttaatatag
      601 atataataca aaaaggcaaa gatattttca aaacattttt
//
LOCUS       DAZKBH010000279          635 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000279 DAZKBH010000000
VERSION     DAZKBH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.295"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..270)
                     /locus_tag="ACG8OO_002987"
     CDS             complement(<1..270)
                     /locus_tag="ACG8OO_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046788.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ACG8OO_002988"
     CDS             complement(276..>635)
                     /locus_tag="ACG8OO_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFP9046789.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAZKBH010000280          616 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000280 DAZKBH010000000
VERSION     DAZKBH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.297"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..584)
                     /locus_tag="ACG8OO_002989"
                     /pseudo
     CDS             complement(1..584)
                     /locus_tag="ACG8OO_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
BASE COUNT          175 a          112 c           82 g          247 t
ORIGIN      
        1 gtaataataa gttgacttcg ctaattgtgc aattgaaagt aatagattca atttaaattc
       61 ttttttgagc tgaaggattg ttttaacctt gatttcttct tgctcaaatc ttgttcttga
      121 atcaaggttt ctaacttttt taaatatgcg ttctctgctc ttaaacgaat aacttcttct
      181 tcaagagact catctttaag ttttttagga atgtttagct tggaattcat actaattttt
      241 ctgccccttt tttggctctc aagtgaagaa gcaccgcctt cttcatattg ctctatccat
      301 ttacttagag ttctatttga accgatatta aattttttag cagtttcttg gatagaaaga
      361 ccatttgttt ccatatattc tataacatca agtttaaatt ttgtagtgta gcttttgcca
      421 cctccaacca agccttccca accatgatag ttataaatcc ttacccaatg tctaaccagt
      481 gtacgattta tttgatattt atgtgcaaga tatttgtagc cgccttcgtt atttaaatag
      541 tctgaaacta cttttttctt aaaaacaaat gtatatttcc gcaaaaaaag cacccctttt
      601 aattagattt ctagtc
//
LOCUS       DAZKBH010000281          592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000281 DAZKBH010000000
VERSION     DAZKBH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.298"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..553
                     /locus_tag="ACG8OO_002990"
     CDS             <1..553
                     /locus_tag="ACG8OO_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046790.1"
                     /translation="IKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGY
                     GIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSST
                     LLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRL
                     SEEKILLPENWAELRKLVEYGII"
BASE COUNT          223 a           82 c           89 g          198 t
ORIGIN      
        1 tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca tacaatcaat
       61 ctttgggata gaagaagttt ttgtcggaga taaatgtatc ttttttgatc aacctttatt
      121 atctgatggt tatggcatag atgaaacaga tattttcgct aaagtaaata gtatattttc
      181 tggaaaaaaa gaaactttaa ttaagcttca tcctagagca aggacaaatc gttatggtga
      241 aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa atgacttctc
      301 ttcaacactt ttgattagtc ctatatctac aatttctttt tcaccatcta tgatgtttga
      361 aaagacgatc aactctatta tgttggctaa attaatttta aatgaatata cagcattatc
      421 tgaagataaa aaagaaacat tgacaaatat tgttcttttc tgtgaacatt ataaccgtct
      481 atcagaagaa aaaattttac tcccagaaaa ctgggcagaa ttaagaaagt tggtagagta
      541 tgggattatt taacacactt tccaagtcaa aattagttaa gtccagtttc tg
//
LOCUS       DAZKBH010000282          527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000282 DAZKBH010000000
VERSION     DAZKBH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.299"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>527
                     /locus_tag="ACG8OO_002991"
     CDS             <1..>527
                     /locus_tag="ACG8OO_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFP9046791.1"
                     /translation="LTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNKFKQGK
                     TALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVSCGMRT
                     LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPHFNSEFGHLEG
                     DTIVGIHHKSAVITLV"
BASE COUNT          186 a           95 c          102 g          144 t
ORIGIN      
        1 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
       61 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
      121 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
      181 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
      241 cttggttgga cgcctgacgt cattatcggg cgaaaagaaa ggcctgtttc atgcggtatg
      301 agaacacttt atcgtttatt ttctaaagga atatttgata ttgacacact accgatgaaa
      361 ggtaaaagaa aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca
      421 atccatgatc gacctgataa ttatcctcat ttcaattctg agtttggtca ccttgaaggt
      481 gatacgatcg ttggcattca tcataaaagt gccgtcatta ctttagt
//
LOCUS       DAZKBH010000283          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000283 DAZKBH010000000
VERSION     DAZKBH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.300"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACG8OO_002992"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACG8OO_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9046792.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAZKBH010000284          516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000284 DAZKBH010000000
VERSION     DAZKBH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.301"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..>516)
                     /locus_tag="ACG8OO_002993"
     CDS             complement(107..>516)
                     /locus_tag="ACG8OO_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFP9046793.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          172 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccat
//
LOCUS       DAZKBH010000285          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000285 DAZKBH010000000
VERSION     DAZKBH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.302"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8OO_002994"
     CDS             complement(<1..132)
                     /locus_tag="ACG8OO_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9046794.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZKBH010000286          485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000286 DAZKBH010000000
VERSION     DAZKBH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.305"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..259)
                     /locus_tag="ACG8OO_002995"
     CDS             complement(62..259)
                     /locus_tag="ACG8OO_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9046795.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DAZKBH010000287          484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000287 DAZKBH010000000
VERSION     DAZKBH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.306"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          159 a           96 c           57 g          172 t
ORIGIN      
        1 gctaaatcac tctacaataa cggcgaaagc acaaaaaata aaaaaattat tacgttatta
       61 aaaaaacaga tgaaagatca ggttttatta gatctcattc tttcatcact tgaagggatt
      121 taatgaacta ctttttcctc tttgtcgttt ttatatattc gtattccttc aaacagtagg
      181 atagcctctt tcaatgcttg atatttgaac tgtccgttac ttaaaaattt agatgtgccg
      241 ccctcttcct agaacatatt cttttttact ttttcattaa ttgacagacc aatcgaacga
      301 aactgtaaat ttcgatagga taatgattgg tcaaactcat tcattttcaa gacatctcga
      361 ttcatattca agataggaat ctttttatca acatcacttt attcgtattt tcctctacca
      421 taaaagcttc acgctccaca aaattgtttt atacactact attcagccca ttcaacgacc
      481 aatg
//
LOCUS       DAZKBH010000288          471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000288 DAZKBH010000000
VERSION     DAZKBH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.307"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          124 a          102 c           98 g          147 t
ORIGIN      
        1 cccactctct cgcaagtttc tgttctggac agccccaaga acactggggc gttggccgtg
       61 gggttgattc tccccctctt tcaaaagagg ctgaaagagg gggttagggg ggagttagtt
      121 ttgagacccc aagactgggg taggggttga ttttttccct tttaaatttt atgctactac
      181 tatttttagt agtgtattct ttatctgttg gctgtcggcg cttggtatgt tattcggaga
      241 cttcccgctt aggtttttga ccattttctc tcataagtgg cggtttttcc acgttgagag
      301 aaaatggtca aaaacctgga agtcggagaa tgcctttcat accaagcgcc gacagccctc
      361 cccccactta ttacatataa cctttaaata atctctaaat aaccctcaaa taatccttac
      421 taatactgaa acgtaaaaaa aataaacgga tttcaaacaa aatacgttta t
//
LOCUS       DAZKBH010000289          468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000289 DAZKBH010000000
VERSION     DAZKBH010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.308"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(315..>468)
                     /locus_tag="ACG8OO_002996"
     CDS             complement(315..>468)
                     /locus_tag="ACG8OO_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9046796.1"
                     /translation="QLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIV
                     NEHTIQ"
BASE COUNT          155 a           68 c           55 g          190 t
ORIGIN      
        1 agaaaaaata tagaattaca agtcgttcaa aagtttaatc acctgttatt attactaact
       61 agtttccata tgttatcatc ataattactt cttgataata attcatatta caaaattatt
      121 cggtaccttt tgtcaaatcg aaattttcat atcaataata agcgagaata ataaaaagac
      181 tactctttcg agtagctata tactgatgtc atcaaaaata gaaaaggaaa cggtgtttag
      241 attgtataat attatgacat ttatattcta tctcattatc tccaaacata ccatttgtta
      301 aattaaaact atatctattg gattgtatgt tcatttacaa tttgttgtgc tttatttgcg
      361 gtatcctcta catcattttt agctttttga agcttttcaa tgtaattatt caaactacta
      421 ttttttgact tttcttcttc tagcttttgc tgagtagctt gcagttga
//
LOCUS       DAZKBH010000290          464 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000290 DAZKBH010000000
VERSION     DAZKBH010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.309"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..394
                     /locus_tag="ACG8OO_002997"
     CDS             95..394
                     /locus_tag="ACG8OO_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9046797.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          177 a           57 c           92 g          138 t
ORIGIN      
        1 acaatccagt aatgatttaa aaaaatctag atgtagcgta tactttgttt atagaatgta
       61 gtgggaagaa atatattttt gggaggaaat cgtgatgtct aaaagtgaag aaatagaaaa
      121 catggtacta aaattatatg atctcgtttt aaatccagag atcaaagaaa aggaacgtat
      181 acttttgata gacgcaaaga caggcttaga aaaaggtcag tattatccta aagtcataaa
      241 taatttggaa cgtagtttac gtccgttagc aatcagggga gaattgtcta aaccagtatc
      301 accgttttat atggagatat caacaatagg aaaatttgaa aaagaattag gtcgaggaat
      361 ggcttcggca ccgattactt ttgggcattt ataaaaacga gttattcatc tcaatgaatt
      421 aaaaagtata gtcaatctca taatgcaggc taggttcatt tatt
//
LOCUS       DAZKBH010000291          452 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000291 DAZKBH010000000
VERSION     DAZKBH010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.312"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>452
                     /locus_tag="ACG8OO_002998"
     CDS             <1..>452
                     /locus_tag="ACG8OO_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046798.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAZKBH010000292          443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000292 DAZKBH010000000
VERSION     DAZKBH010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.313"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..441)
                     /locus_tag="ACG8OO_002999"
     CDS             complement(<1..441)
                     /locus_tag="ACG8OO_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9046799.1"
                     /translation="MGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVSAA
                     IPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGYVV
                     FQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIG"
BASE COUNT          192 a           67 c           50 g          134 t
ORIGIN      
        1 accaattgta agtaaataaa tcatgaatat agcagtaagt ttagaactgg actgttcaac
       61 aatcctattt atagctaatt ttctatataa ccaaataaac atcagcaaaa acaaagaatc
      121 aagcattatc tggaatacta cataaccata cccttttatc ccttcaaatg aaaacatata
      181 cgtcataacc atggaagtaa taatagatct gaaatcatca ataagcatac ataatagtaa
      241 tttatttaaa agcactatat tagttttctt cttttccttt aatataaaat aacttccgat
      301 tataggtata gctgcggaaa catcagtaaa taattcaaca aaaaaagtga gtggtatcaa
      361 taaacataca aaaagatatt ttcttttgtt aagatcagta ttagtcatat aaaataagag
      421 aaaaaagaaa aagtttccca cga
//
LOCUS       DAZKBH010000293          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000293 DAZKBH010000000
VERSION     DAZKBH010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.316"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG8OO_003000"
     CDS             complement(<1..339)
                     /locus_tag="ACG8OO_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9046800.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAZKBH010000294          413 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000294 DAZKBH010000000
VERSION     DAZKBH010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.317"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACG8OO_003001"
     CDS             <1..390
                     /locus_tag="ACG8OO_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9046801.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          113 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caa
//
LOCUS       DAZKBH010000295          409 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000295 DAZKBH010000000
VERSION     DAZKBH010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.318"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..379
                     /locus_tag="ACG8OO_003002"
     CDS             <1..379
                     /locus_tag="ACG8OO_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFP9046802.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DAZKBH010000296          399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000296 DAZKBH010000000
VERSION     DAZKBH010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.319"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DAZKBH010000297          398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000297 DAZKBH010000000
VERSION     DAZKBH010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.320"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..>398
                     /locus_tag="ACG8OO_003003"
     CDS             122..>398
                     /locus_tag="ACG8OO_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP9046803.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT          129 a           71 c           72 g          126 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagt
//
LOCUS       DAZKBH010000298          352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000298 DAZKBH010000000
VERSION     DAZKBH010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.322"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..347)
                     /locus_tag="ACG8OO_003004"
     CDS             complement(<1..347)
                     /locus_tag="ACG8OO_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFP9046804.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEA"
BASE COUNT          119 a           71 c           44 g          118 t
ORIGIN      
        1 ttagcttctc ctactgttga gcattgtcct aaaatccaag gaataaattc aaaaggagat
       61 acattgtctt tcccttcttg tatttcttta taatcagcat acccagaaaa atttagccca
      121 gccatactca atcctttttc atttgtcgca tcgtaataaa gagggtagtc agctatacca
      181 gcggcaatac caatcattgc ataatgagta tccaaatcat ttacctttcg aaaattcaac
      241 ttataatttc ttggagtaac agtaactact tcattgtaag atatttcata atcaaaattc
      301 cttccaaaat aatgatcact tgttacataa gtaatagacg tacacatagt tt
//
LOCUS       DAZKBH010000299          345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000299 DAZKBH010000000
VERSION     DAZKBH010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.323"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..321)
                     /locus_tag="ACG8OO_003005"
     CDS             complement(<1..321)
                     /locus_tag="ACG8OO_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9046805.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           97 a           55 c           63 g          130 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaat
//
LOCUS       DAZKBH010000300          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000300 DAZKBH010000000
VERSION     DAZKBH010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.324"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACG8OO_003006"
     CDS             <1..>341
                     /locus_tag="ACG8OO_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFP9046806.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZKBH010000301          340 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000301 DAZKBH010000000
VERSION     DAZKBH010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.325"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..214
                     /locus_tag="ACG8OO_003007"
     CDS             17..214
                     /locus_tag="ACG8OO_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP9046807.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            216..>340
                     /locus_tag="ACG8OO_003008"
     CDS             216..>340
                     /locus_tag="ACG8OO_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9046808.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQ"
BASE COUNT          131 a           35 c           68 g          106 t
ORIGIN      
        1 taggggtgat tagattatga aacatttaaa aattttgtct attaaagaga cacaacttat
       61 ctatgggggt accactcata gtggaaaata ttatggaaat ggagtgtatt gcactaaaaa
      121 taaatgtacg gtcgattggg ccaaggcaac tacttgtatt gcaggaatgt ctataggtgg
      181 ttttttaggt ggagcaattc cagggaagtg ctaaaatgaa aaaaaatgct aagcaaattg
      241 ttcatgaatt atataatgat atatctataa gtaaagatcc taaatattct gatattcttg
      301 aggttttaca aaaggtatat ttaaaattag aaaaacaaaa
//
LOCUS       DAZKBH010000302          338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000302 DAZKBH010000000
VERSION     DAZKBH010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.326"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..338
                     /locus_tag="ACG8OO_003009"
                     /pseudo
     CDS             1..338
                     /locus_tag="ACG8OO_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612034.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DAZKBH010000303          331 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000303 DAZKBH010000000
VERSION     DAZKBH010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.327"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>331
                     /locus_tag="ACG8OO_003010"
     CDS             <1..>331
                     /locus_tag="ACG8OO_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HFP9046809.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DAZKBH010000304          323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000304 DAZKBH010000000
VERSION     DAZKBH010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.328"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8OO_003011"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8OO_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFP9046810.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAZKBH010000305          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000305 DAZKBH010000000
VERSION     DAZKBH010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.329"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG8OO_003012"
     CDS             complement(<1..>321)
                     /locus_tag="ACG8OO_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9046811.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZKBH010000306          309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000306 DAZKBH010000000
VERSION     DAZKBH010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.330"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..>309
                     /locus_tag="ACG8OO_003013"
     CDS             51..>309
                     /locus_tag="ACG8OO_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP9046812.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT          120 a           41 c           61 g           87 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggc
//
LOCUS       DAZKBH010000307          308 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000307 DAZKBH010000000
VERSION     DAZKBH010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.331"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..>308)
                     /locus_tag="ACG8OO_003014"
     CDS             complement(76..>308)
                     /locus_tag="ACG8OO_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9046813.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DAZKBH010000308          301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000308 DAZKBH010000000
VERSION     DAZKBH010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.332"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..301
                     /locus_tag="ACG8OO_003015"
                     /pseudo
     CDS             1..301
                     /locus_tag="ACG8OO_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           60 c           57 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 g
//
LOCUS       DAZKBH010000309          296 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000309 DAZKBH010000000
VERSION     DAZKBH010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.333"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>296)
                     /locus_tag="ACG8OO_003016"
     CDS             complement(<1..>296)
                     /locus_tag="ACG8OO_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFP9046814.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKK"
BASE COUNT           99 a           53 c           27 g          117 t
ORIGIN      
        1 atcttttttt cattttttaa aaaatacctt ttaaatttct tatagtcctc tgaaatcaaa
       61 atatagcgtg atggatactc atcaaaagaa gaatcgtatt cttcctttaa aattcccgat
      121 ttacttaatt tttctagtac atcaaatact ctatcactca agtaactcct taaattgtta
      181 tcactttccc attgaactac ttttctataa gttgaattat cagttgaaaa gatagaattt
      241 tcatccataa acgcatatat tagcagcaat ctttcatcat tgtcaaattc tattgg
//
LOCUS       DAZKBH010000310          292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000310 DAZKBH010000000
VERSION     DAZKBH010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.334"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..185
                     /locus_tag="ACG8OO_003017"
     CDS             <1..185
                     /locus_tag="ACG8OO_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9046815.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DAZKBH010000311          282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000311 DAZKBH010000000
VERSION     DAZKBH010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.336"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACG8OO_003018"
     CDS             <1..>282
                     /locus_tag="ACG8OO_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFP9046816.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAZKBH010000312          270 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000312 DAZKBH010000000
VERSION     DAZKBH010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.338"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>270
                     /locus_tag="ACG8OO_003019"
     CDS             <1..>270
                     /locus_tag="ACG8OO_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9046817.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DAZKBH010000313          255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000313 DAZKBH010000000
VERSION     DAZKBH010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.342"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           46 c           39 g           91 t
ORIGIN      
        1 aattggagtg gtagttaatt agtgacttat ttttgatttt atagcactga tactataaaa
       61 tatagatatc atcatattac acaatcttaa tactaactta aaaaatatct cctttcacaa
      121 gtatggtgat aaaattcgtt ccgggctacc tttttaggta gcctacttta atctttatac
      181 ctttctggat caacgaaagt atactttata tagtcataac gccgatgatc gctacgtgcg
      241 tccggcacgt cagtc
//
LOCUS       DAZKBH010000314          252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000314 DAZKBH010000000
VERSION     DAZKBH010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.343"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..>252)
                     /locus_tag="ACG8OO_003020"
     CDS             complement(32..>252)
                     /locus_tag="ACG8OO_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP9046818.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DAZKBH010000315          250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000315 DAZKBH010000000
VERSION     DAZKBH010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.344"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..>250
                     /locus_tag="ACG8OO_003021"
     CDS             77..>250
                     /locus_tag="ACG8OO_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9046819.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLV"
BASE COUNT           98 a           29 c           45 g           78 t
ORIGIN      
        1 attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt tttggtagtt
       61 ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag tcatacatga
      121 attatacaat agtttaatga cacgacctaa tcagacgaaa gcattattag atattactga
      181 tgttttattt caggtatata aaaaaattga tacagcaaaa tatcctgaaa ttcttgtaaa
      241 taaattagtt
//
LOCUS       DAZKBH010000316          239 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000316 DAZKBH010000000
VERSION     DAZKBH010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.denovo.345"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>239
                     /locus_tag="ACG8OO_003022"
     CDS             <1..>239
                     /locus_tag="ACG8OO_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9046820.1"
                     /translation="AIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSN
                     WKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           84 a           46 c           43 g           66 t
ORIGIN      
        1 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
       61 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      121 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      181 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAZKBH010000317        10933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.088, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000317 DAZKBH010000000
VERSION     DAZKBH010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.088"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            249..485
                     /locus_tag="ACG8OO_003023"
     CDS             249..485
                     /locus_tag="ACG8OO_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046821.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            492..2444
                     /locus_tag="ACG8OO_003024"
     CDS             492..2444
                     /locus_tag="ACG8OO_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9046822.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2516..3013)
                     /gene="dfrG"
                     /locus_tag="ACG8OO_003025"
     CDS             complement(2516..3013)
                     /gene="dfrG"
                     /locus_tag="ACG8OO_003025"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9046823.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3288..4415
                     /locus_tag="ACG8OO_003026"
                     /pseudo
     CDS             3288..4415
                     /locus_tag="ACG8OO_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            4513..4701
                     /locus_tag="ACG8OO_003027"
     CDS             4513..4701
                     /locus_tag="ACG8OO_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP9046824.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4759..5112)
                     /locus_tag="ACG8OO_003028"
     CDS             complement(4759..5112)
                     /locus_tag="ACG8OO_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9046825.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5643..6065
                     /locus_tag="ACG8OO_003029"
     CDS             5643..6065
                     /locus_tag="ACG8OO_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9046826.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6062..6292
                     /locus_tag="ACG8OO_003030"
     CDS             6062..6292
                     /locus_tag="ACG8OO_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9046827.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6790..6990
                     /locus_tag="ACG8OO_003031"
     CDS             6790..6990
                     /locus_tag="ACG8OO_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFP9046828.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7017..8210
                     /locus_tag="ACG8OO_003032"
     CDS             7017..8210
                     /locus_tag="ACG8OO_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9046829.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8278..9843)
                     /gene="guaA"
                     /locus_tag="ACG8OO_003033"
     CDS             complement(8278..9843)
                     /gene="guaA"
                     /locus_tag="ACG8OO_003033"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9046830.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9902..10825)
                     /gene="coaA"
                     /locus_tag="ACG8OO_003034"
     CDS             complement(9902..10825)
                     /gene="coaA"
                     /locus_tag="ACG8OO_003034"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9046831.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
BASE COUNT         3641 a         1939 c         1972 g         3381 t
ORIGIN      
        1 gtcttgcata ttaagtagtt cagttttgga gtacaaaaag tgaaaatgta aacctaaatg
       61 agtatcaaat tgcaactgaa tttgatactc gttttttatt gttaaatcaa cgtttgttgt
      121 taatttacac cacaaaatgt aagttaaaat gtatataaaa atgcgaacgt gaaataatta
      181 ttgtaggaat gcaaattggt gtgttaaaat ggttacaatt tactaagcaa aaaggggtgc
      241 attttcctat gcaatttctt gagccaaaaa ataagaacgc taagtctgtg gattgggaaa
      301 tatcagagca agtaagggtg atagttaagc aatacgctga atatgcagag cgtactgaaa
      361 gcgaagcagt ggatgaattc ttattgaaca ttttagacga taagaagttt attgaatgga
      421 ttgccaacaa gcgaagtaat aagcggattg ttgaaaaaat gggtatcaaa gatagagtgg
      481 gttgatagac aatgaccaag cttaaaagat tagccacgaa ggaagatgaa attgtagatg
      541 ttaaaattcc gatttcaaac gaagaattaa aagatagggc taaacaatat gatttattaa
      601 ctccaaaacg atttgcaaca aggtataaca aaatgttgtt cttgccaacg tcttttaaat
      661 ggaacggttc tgaatatccc attcaatata attactgcat caatccgttt tgttgtaatt
      721 ttgggaaaga acagcacaaa ttcaaagatg taaaagggaa accaagtcga tataaaatga
      781 ctggtagttc caaagataaa ggtcataaag gtatgtactg caatgacaat cctattggca
      841 gaggtgtatc gcaaaactgt acagtaaccc ctttatctaa ttggtctgtt gttgaagaaa
      901 ttaaacgcct tatagaaatc aatagcatcc aagatgttga gccagattat caatttcata
      961 aagaaggctg ctcggaagaa gaatcaaccc cattcaatga gccgaagcaa ttttacaaaa
     1021 gggggaaaag taggggcaaa tctcaacgct atcaatgtaa ggcgtgtaag aagttcacta
     1081 atgttctacc aaaaagagaa gaaaccacta cttatcacca acaaaagaac accattcttc
     1141 caatgttcgc taagatggtt gttggaaggg tttcagtcag tcgtacttgt gatattttag
     1201 gaattggagt tggaacctac tatcataagc tggagtggct gtataggcgt tgtttggagt
     1261 ttttagaacg atatgagaca caaccccttc aaacaaagaa atttaacgag atgtggctta
     1321 atacggacaa gatgcactac tacctaaata acgtccgtaa gaaaggacaa ggttcaaaga
     1381 aatatacggg gtttgaagac cttaatatgc aaacgtatat agttgtatct gctgaggtct
     1441 tatcaagata tgtgttccgt tctgatgttg cctatgattg gaatatttca atggatgaat
     1501 taaatgaaga tacaagaaaa ttcaaagaag accatctcaa tacttttagt cgtaaaaatg
     1561 ataggttaga ttggtcttat tatccacaag aaccatccgc taacgatagc gaaaatagga
     1621 atgcttatct tcacgaatta gggaaaatta ctaataggag tagatttgtc gatggtttaa
     1681 atgttgatgc tccttatact acaactgctc actattggtt aattaaacaa atggttaatg
     1741 ccgacgaatg gcgaatgatt agtgatgatg acttctctat tagaaacgcc ttttatagag
     1801 tattcacgaa agaattaagg ttgagcgatg cacatcattt tatttgccaa gttaacaaga
     1861 cgaaaagcag aaaacaatgc ttaaaagaat tcggacaagc aaaagcagaa ctgctcgatt
     1921 ggggagatat cagaggattc aaaacaaagt ttctacggac tttggcatct cattatctaa
     1981 ctgaattact aacaagtcat caattccacg aagaggctat tagtaaagat ggagaaagat
     2041 atcgcaaata tgccgataac cctatcaaac atccattagc cactaaggat aaaggatttt
     2101 attcagttga ttgtagaacg gatttatctg ctctggaacc aaacgaaatc gcaaagatgc
     2161 tattaaatgt taatgaccat tcgaccaata gctttattca acagataaga cgttatatat
     2221 catctcttga aagaccatta acaacagctc gtggtgataa gaagagctat atttacgcta
     2281 acttcaatcc gaagtatgct caatttgcga ttacaattct aagaacttac tacaactttt
     2341 gtcgcccttt taaatcagcg gataaaaagg ttttgacacc tgctcaaaga ttagggatta
     2401 cagataaaca atttgattgg aaagatataa tttatttcaa atgacaaaaa gagcttggag
     2461 taatcccaag ctcttgtgct ttatctttac tgttcttctt caattaacgc acccgttaac
     2521 tcaataagtt ttttctttca tatacatgaa aatagtagtt atacggattt ttatcattct
     2581 ttatcccttt ttgggcaaat acctcattcc attcctcata attcacttct ggaaaaaaag
     2641 tatctccttc gaattcatga tgtatttttg tgatgtacat tttctcaaca taagggaaaa
     2701 acaaattata aatctgttct cctccgaaaa taaaaatttc ttcttcgttt ttacataact
     2761 caaaaacatc ttctattgaa tgaacaattt cacaaccatt aaaggtaaac cccttatctc
     2821 tcgtcagaat aatatttctt ctgtcaggta aggctcttcc gattgattca aggttcttcc
     2881 tacctaatat tatcggatgt ccctttgtag tatttttaac atattcccag tccttgggaa
     2941 tcctccaagg aatgtcattc tctttgccaa tcactctatt cttatccatc gcagcaatca
     3001 aagaaacttt catttataac acagcctttt taactaattt accatctaat ttcttatttc
     3061 tatcaaaaaa ccttttctca ttatcctgcc cgttagttga ataagcaatt ttttctataa
     3121 gttatattat cataatacca caaaatcaca aattttcatt caattatcat tttcaattgc
     3181 attttataaa tcattttgta tgtaatttgg tatgtaagtt aaaatgaaaa aagtagtcca
     3241 ttttgaagta cattttgtca ttttgtactc caaaactgaa ctacttactt gcatatatac
     3301 gcctttatag tggagtacta catttacgag attcggttag agtatcagaa aaagaaaaaa
     3361 taaaagttac agaaatgtat acttcaataa atggtgaatt atgtaagatt gatagagctt
     3421 attctggaga aattgttatt ttgcaaaatg agtttttgaa gttaaatagt gttcttggag
     3481 atacaaaact attgccacag agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa
     3541 ctgttgaacc gagtaaacct gaacagagag aaatgttgct tgatgccctt ttggaaatct
     3601 cagatagtga tccgcttcta cgatattacg tggattctac gacacatgaa attatacttt
     3661 ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg
     3721 tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag
     3781 aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg
     3841 tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact
     3901 taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat
     3961 tatatggttg gaatgtgacg gattgtaaaa tctgttttaa gtacggttta tactatagcc
     4021 ctgttagtac tccagcagat tttcggatgc ttactcctat tgtactggag caagccttta
     4081 gaaaagctgg aacagaattg ttagagccat atcttagttt taaagtttat gcaccacagg
     4141 aatatctttc acgggcatat aacgatgctc ccaaatattg tgcaaatatc gtaaatactc
     4201 aactgaaaaa taatgaggtc attattattg gagaaattcc tgctcgatgt attcaagatt
     4261 atcgcaatga tttaactttt tttacaaatg ggcttagtgt ttgtttagca gagctaaaag
     4321 gatatcaggt taccactggc gaacctgttt gccagacccg tcgtctaaat agtcggatag
     4381 ataaagtaag atatatgttc aataaaataa cttagtgcgt tttatgttgt tatataaata
     4441 tggtttctta ttaaataaga tgaaatattc tttaatatag atttgaatta aagtggaaag
     4501 gaggagattg ttattataaa ctacaagtgg atattgtgtc ctatttgtgg aaataaaaca
     4561 agactacgaa tacgagtgga tactatactt aaaaatttcc ctttatactg ccccaaatgt
     4621 aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa taacaatcaa agagccagac
     4681 gccaagacgc agagccgata atttgagaaa tgaaactctc atcttatcgg ctctttttgt
     4741 ttatctgaat tttactgact agccttcaat atttcttgct tcattgatac cttttgctag
     4801 tgcttccatt aaggatagtt ctttgtctgt aaagctatcc atgtattttt ctacctgtaa
     4861 ccttcgagtg cttttaatca aattatctgt aggtaagaaa aattcatcaa tagatacatg
     4921 gagtaatgat acaagatcat acagcacttg tgtacttggg tgttgtcctt tgttttctat
     4981 atttgttaaa taacgtgggt caatttcaat cattgttccc acttgttcac gagtgagacc
     5041 ccgtttcatt cgagcctctt taatcgctaa accaacggct ctaaaatcgt atttatcttc
     5101 ttttttacgc atactaaatc acctctatac attttactgt tcctatataa ccataaacag
     5161 gtatagaaaa acatgttata tagtttatga gttcctatag aacactgtgg aatttattaa
     5221 tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt atttacctat acaactttat
     5281 aaaatttagt catactccta gaaagtagat aaaattggcg acttaatgga gggaaaagtc
     5341 gccatgagaa agataatctg tcatctagtt ctcttaatta agttcttatc aaaaaaactt
     5401 atccacctgt actggataga tatgaccatc aatgtggttt tgcagattac atagatagaa
     5461 aaatcaaagc atatgaacag agaaaacaat ctgtttatgt gcttttttgg tcacttaata
     5521 aatatctttt aggatttctc ttcatttatg caacaaatcc cccgttcgtg tcggggttta
     5581 gtatgaaggg agataaagaa gcacatgcca ctccctttca cgacagaaag ggggtgaaaa
     5641 gcatgaaacc atcttctttt caagaaacca tagaaaatca gtttgactat atctgcaaac
     5701 aagttattga agatgaacga aaagattact tcaaacatct agacagacta tcagcaaaag
     5761 aagtctcgtt ttctgatata gataactacc ttgtcaactc tttttctact gttgatacgt
     5821 acgtcactga tttccaacta tttaaattat ttggattaag aatcaatgtt gaaagtgatt
     5881 tattaagtga agccctgcgt catctttcag agaaaaaacg gaacattatt ttacttcatt
     5941 actttatgga tatgagtgat gtggagattg cggagttact aaatttgaat cgttcgactg
     6001 tttatcgtca tcgaataagt ggactagcca tgattaaaga attcatgaag gagtgtaaag
     6061 aatgaaaaca gcttatccaa gggttccctt ccccctcatt gtaaaggcga cagatggcga
     6121 cgtggaagca attaatcaga ttgttaaaca ttatcgaggg tatacctcaa aacgttcatt
     6181 acgtcgcatg acagacgaat atggaaattc tcatatggtt attgatgaaa ccctacgtgg
     6241 cagaatggaa acgaaactca tctctaagat tttatcgttc gaaattaaat agtcccattt
     6301 tttcgtggaa gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt tgttattcca
     6361 tcaaagcata ttgtgtgttc agtaggtttt gataggctaa caagccaaac gttctttgac
     6421 aaccaaataa aggcaatcga atacgtttcg gtaagaaaag agctgatgaa ctaacacgcc
     6481 atgacctaca aaattagtga gcgattcatg ttagtaattc gaaagtaatc tttagcagga
     6541 ttgctgccat gactttctta tcgtgataat gatactcccg tatagccata gtccgagcgt
     6601 tagaagcgtc acaggcaatg ggtacggcta catgagaacc atgcagaagt gaaattcctg
     6661 tgagctttgc tagagctgtt cgattgctaa gatattcttc tcttgtcagt gtattattat
     6721 aaaactattt gatataatta taggtgaata ttggagtttt cctagcctat aattgggaaa
     6781 ggagtaataa tgataacggt cacaaaaaag gatttagttg agctaggata tggaacttct
     6841 ttttcagcag acatcataaa aaaagcaaaa gaattgatga ttacaaaagg gcatatctac
     6901 tatcaatctc gaaagctaga tagagtccct cgtgaagctg ttgaagaaat tctaggaatt
     6961 cggttttcaa atgaaaacag gtctgagtag tttttgcaca gtaaggagta aaaactatgg
     7021 ttaaagaccc aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca aatctaggtt
     7081 atgattctaa agggaaaaaa attcaaaaat atcgtagtgg attcactact aaaaaagaag
     7141 caagagaaga atattcgaaa ctgctattga tgaaaccaga agaattaagt gagaataagg
     7201 ataaaatgac gtttaaacat tatatctttg aaattttcct tccatggtat aagacacagg
     7261 taaagctcag aacatatgaa aatcgcttac caactattaa gaagcatttc tcttattttg
     7321 ataaaatggc tgtttccgat attgagccta ttgatgtgca aaattggcaa ctaaaattat
     7381 caaagaagtg taagtcttct tatgtaagag ctgttcaagg actattttca gttgctatgg
     7441 atcgagcaat tgtccttggg ataaccacca caaatccatc taagattatt ggcaatgtga
     7501 aaaagcaaaa gtctaaaatt gaattttgga caaaagaaga atttgaaaaa gtaatctctc
     7561 taatttacaa agaagattac tatcaacact ttttatttat ctctctgtgg tttttattta
     7621 tgactggaat gcgaattggt gaagctactg ccattcaatg ggaagatatt gattttgact
     7681 caggagtact aaccatcaac aaaacactct attacaagaa tcaatcgaat tatagatttg
     7741 ttgaaccaaa aacaaaggcg agcgttcgtc atattgtatt agataaatgc acattaactt
     7801 atttatccga atggaaggaa gtacaacaaa atctaattca gacagacttt gtaatgagct
     7861 ataacggtat tcctacccaa aaacatacac tatcatatgc tattgaacgc tatgctaagc
     7921 tggctggtat ccatcgaatc agaatccatg ggttacggca ttctcatgct tccttgttaa
     7981 tcagtatggg agaaaatcca cttatcatta aagatcgtct aggacacgaa gatattgaga
     8041 cgactttagg aacgtatgga catctttatc ctaacagtaa tttcgaggta gctaacaaat
     8101 taaatggagc catttccttt aaagaagcaa aacaaaatta tgatacttca ccaaagaatc
     8161 aatttactgt agactacttg cgaaacaaag aaatgaaaaa agtgcaataa aagtgcagta
     8221 aaaagataag aagagccaga aacccttggt ataagaggat ttctagctct cctacaacta
     8281 ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg
     8341 ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc
     8401 aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta
     8461 agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg
     8521 ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc
     8581 gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg
     8641 accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc
     8701 acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc
     8761 tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc
     8821 gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag
     8881 ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg
     8941 ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat cgactttgat
     9001 gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg
     9061 tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag
     9121 aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc
     9181 aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc
     9241 ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg
     9301 acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc
     9361 aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa
     9421 gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg
     9481 atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga
     9541 accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc
     9601 taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc
     9661 gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg
     9721 gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata
     9781 ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt
     9841 caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat
     9901 gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta
     9961 tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt
    10021 tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat
    10081 aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc
    10141 atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga
    10201 cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat
    10261 caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa
    10321 caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct
    10381 ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac
    10441 gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac
    10501 gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg
    10561 cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca
    10621 agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga
    10681 tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat
    10741 cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac
    10801 ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat
    10861 tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata
    10921 atctttataa tag
//
LOCUS       DAZKBH010000318         8061 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.111, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000318 DAZKBH010000000
VERSION     DAZKBH010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.111"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG8OO_003035"
     CDS             complement(134..577)
                     /locus_tag="ACG8OO_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFP9046832.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG8OO_003036"
     CDS             complement(795..2135)
                     /locus_tag="ACG8OO_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9046833.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG8OO_003037"
     CDS             2404..3300
                     /locus_tag="ACG8OO_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9046834.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG8OO_003038"
     CDS             complement(3450..4391)
                     /locus_tag="ACG8OO_003038"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9046835.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG8OO_003039"
     CDS             4517..5638
                     /locus_tag="ACG8OO_003039"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9046836.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8OO_003040"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8OO_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9046837.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG8OO_003041"
     CDS             7465..7992
                     /locus_tag="ACG8OO_003041"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9046838.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2671 a         1505 c         1597 g         2288 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag g
//
LOCUS       DAZKBH010000319         4138 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.162, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000319 DAZKBH010000000
VERSION     DAZKBH010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.162"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..1566
                     /gene="msr(C)"
                     /locus_tag="ACG8OO_003042"
     CDS             88..1566
                     /gene="msr(C)"
                     /locus_tag="ACG8OO_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9046839.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1613..2059
                     /locus_tag="ACG8OO_003043"
     CDS             1613..2059
                     /locus_tag="ACG8OO_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9046840.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2258..2773)
                     /locus_tag="ACG8OO_003044"
     CDS             complement(2258..2773)
                     /locus_tag="ACG8OO_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9046841.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2890..4092
                     /locus_tag="ACG8OO_003045"
     CDS             2890..4092
                     /locus_tag="ACG8OO_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9046842.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1229 a          704 c          899 g         1306 t
ORIGIN      
        1 gtgatgcagt ttttttgcaa acagattttt ctgctctttt ttagtatcga taattgttgt
       61 tttacatgaa ataggagtgt aaaacatatg gaaaatttag cagtaaatat aacaaatcta
      121 caagtcagct ttggcaacca gctagaacta tctattgatt ctcttcgtgt ctatcagcaa
      181 gatcgtatag ggatcatagg agaaaatggt gtcggtaaat cgactttgct caaactaata
      241 gccggtgaac tttttcccga tcatgggaaa atccaaacag agatcacctt caactacctg
      301 cctcaattaa cctatctcgc tgaagcaaag gacctaaatt tggaattagc cagtcatttc
      361 cagttaagac tggaagaaac ttcagagcgg aaatggagcg gaggggaaga acgaaagatc
      421 gagttgatac gtcttctttc ttcttatgaa caagggatgc ttctagacga gccaacaacc
      481 catctagata gaaaaagtat tgatcgactg attgaagagc ttcgttatta ttatggtacg
      541 ctggtttttg ttagtcatga tcgctatttt ctagatgagc tagcatcgaa aatctgggaa
      601 gtaaaagacg gagaaatccg agagttttcg gggaattata gtgcctatct cactcaaaag
      661 gaattggaga aaaagactca gctacgagaa gcagagtcga tcatgaaaga gaaaaaacga
      721 ttggaaaaat cgatccaaga aaagaaaaaa caagcggaaa agttggaaaa agtgtccagt
      781 aaaaagaaaa agcaacaaat cagactggat cggttgtctt cctctaaaca aaaagacagt
      841 gtacaaaaag ccatccaaaa gaatgcgaaa acattagaga gaagactcca aaaaatagga
      901 gaaacaacca agccgcagca gatgaaacaa atccgttttc cagtaccaaa atctcttgaa
      961 ctccacagcc gctatcccat catgggacaa aatgtccaat tggaaagaag tgggagaaca
     1021 ttactggtaa atggtgattt tcagttttct ttaggtaaaa aaatcgcgat tgtcggtgaa
     1081 aatggttcag gtaagacaac cttattagaa catatccgca aacaaggaga aggaatcctt
     1141 ctctctccga aagtaagctt tcaagtatat cagcaaaagg gttatcaaat gacatctgaa
     1201 gaatccatca ttcgttttgt catgagacaa acagagtttt cagaatcgct tgtccgtagt
     1261 ttgctgaatc acttagggtt tgctcaggaa actttgacga aaccgttatg tacattaagt
     1321 gggggagaag cgacccgtct gacgattgct ttgcttttta ctaagccaag taatgtgttg
     1381 ctgttagatg aaccgactaa ttttattgat atggcaacga tcgaagcttt agagaagcta
     1441 atgcaaatat atccgggaac gattttgttt acttctcatg attcttactt tgtcgagcgt
     1501 acggctgatg aagtttatga aataaaaggg cagaaaataa aaaaagtact tacgagaaat
     1561 ttttaaatgt gctacgatga ttttcgtgac aaatttaaag gagtggtaca gattgcctac
     1621 aaataaaaaa gaaggaataa tttttacaac aatgatgtgt tttttgatgg tcttggggat
     1681 gagcgcgtat aatttatggc tgcatcaaga tcttcattta tcaagtttgt tgattggtct
     1741 agtgcctgga ttcgtggtgg ctttcatttt ggatgtcttc atcatcggtg tgctggcgaa
     1801 aaaaattgcc tttaaactac cagtaaatcc agaaagcaag gtccaaatga tcttaactat
     1861 ttcatgcttg atggttttag gaatggtgac atgtatgtct ttattcggtg tgatcatgga
     1921 acaaggaata cctgatcaat tattctctgc ttatatgaat gcttggaaaa tgaatgtgat
     1981 tgccgcttta ccgttgcagt tgatcatcgt tggcccgctt tcaagaagtg tgctaaaagc
     2041 aattcaaagt aatgaatgat ggaacactgt tatttgaaaa aaggccttgg catgcttttt
     2101 gtcatagtct cttgaatgca gttctagaaa aatagtgatc cgaacgatag gttttggcct
     2161 gtcgttcgga tcgctttttc ttttccaact gtattgctct tttattgaag ataatttgct
     2221 cttcaataga tatttctaga aaggttctgt ttatccctta ctcaaaaaac tgctgcattt
     2281 gttctcttac ttgctgacac cattggagtt cctgctgaca aagcataatt ctgcgctgga
     2341 tcacaacata acgtccaaat tcttgtttct tgtctagcga gaggtcattc aatttttttt
     2401 gatttttttg catgattttg ttcagctgtt gttctctagt gaatagcagt ccagtagctg
     2461 ttgttttatc gagcgtcgaa aaagcataga tttttgctag aaattcatct ttttgcgtag
     2521 gagcgggagt ttcctctttg atccattcct ctacaagagc acgtccttca tcagtcaagt
     2581 ggtagatctt tttttgagag tgctcgtctt tgtccaaaac ggtcacataa ccctgctggt
     2641 ctaacttggc aagcgttgta tagatctgac tgtgatgggc ttcccaaaaa attgtgatca
     2701 gctgcttcaa ttcataccca gaacgtggtt tggataaaag cacgcataat aatatatagg
     2761 ctaaagtatt cataaaaatc ctacctctcg tcttattttc tttattatat cacagtaaag
     2821 tttccacgtt ttacatgtgc atattgacat gtaaaataaa tcatgtatga tgctaataga
     2881 gagaaaggga tggaaatatt ggagaatcaa atagatatac aagaacaaac aaatgaagaa
     2941 acaaagggga aaagttcagc gctagttgta gcagctacct gtatgatcgc ttttatggga
     3001 gtgggtctag tagacccaat cttaaaaacg attgctgcac agttaaacgc tacgccagct
     3061 gaaacgaccc tgttatttac tagttacatg ttagtcactg gtgtggtgat gctatttaca
     3121 ggtttcttat ctagtcggat cggtttgaaa aaaacattga tgacaggatt gtttatcatc
     3181 gttgtattcg ctggtcttgg cggattatct gggaatatcg gaatgctgat tgggttacgt
     3241 gccggctggg gtgtcgggaa tgctttattt gtctctactg ctttagcaac gatcgtcagt
     3301 attttagttg gtaacacaga aaaagcaatc atgatgtatg aagcagcact tggctgcgga
     3361 atggctgttg gccctctagt cggtggggtc ttgggcagtg gttcatggcg ttatccattt
     3421 tttggggtag catttttgat gttttgcggt tttatagccc tagcggtact tttacctgaa
     3481 actgagaaac caaagagaaa agtaggcctt tgggaaggtg tcaaagcgtt aagcaatcat
     3541 aaacttcgtt cagttggact aatcgcattg ttatataatt tcggattctt tactttactt
     3601 gcgtatgcgc catttttatt gattggctat tctgaattag aagtcggttt tgtatttttt
     3661 ggctggggtg tgcttctggc tattgcttcg attttcgttg caccggctct tgagcggaag
     3721 tttacgactt atcggacaat tttagcagca tttgtcttat tcattatttg tttgctgtta
     3781 ttaggagctg gaacttctat gccagtcctt gtaccgatca gcattgtgtt agcaggattt
     3841 ttccaaggaa ttatcaatac actgttgacg acgattgcta tggaaatacc aggtctgcaa
     3901 cgaaacgttg cttcgtccag ctatagtttt gtccgctttt tcggtggagc tttagcacca
     3961 tttattgcag gtgtgattgg tgaacatatt ggtgaaagct atgcgtttta ttttgcaggt
     4021 gccattgttc ttatcagtgt atgttgtatc atttatcacc gtaattattt tgctacaaat
     4081 gaagaagaat aagaaatcag aggttgtgaa aaagctgtcc tgcatcaagt cataagaa
//
LOCUS       DAZKBH010000320         4138 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.163, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000320 DAZKBH010000000
VERSION     DAZKBH010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.163"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..1566
                     /gene="msr(C)"
                     /locus_tag="ACG8OO_003046"
     CDS             88..1566
                     /gene="msr(C)"
                     /locus_tag="ACG8OO_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9046843.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELPSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1613..2059
                     /locus_tag="ACG8OO_003047"
     CDS             1613..2059
                     /locus_tag="ACG8OO_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9046844.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2258..2773)
                     /locus_tag="ACG8OO_003048"
     CDS             complement(2258..2773)
                     /locus_tag="ACG8OO_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9046845.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2890..4092
                     /locus_tag="ACG8OO_003049"
     CDS             2890..4092
                     /locus_tag="ACG8OO_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9046846.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1228 a          705 c          899 g         1306 t
ORIGIN      
        1 gtgatgcagt ttttttgcaa acagattttt ctgctctttt ttagtatcga taattgttgt
       61 tttacatgaa ataggagtgt aaaacatatg gaaaatttag cagtaaatat aacaaatcta
      121 caagtcagct ttggcaacca gctagaacta tctattgatt ctcttcgtgt ctatcagcaa
      181 gatcgtatag ggatcatagg agaaaatggt gtcggtaaat cgactttgct caaactaata
      241 gccggtgaac tttttcccga tcatgggaaa atccaaacag agatcacctt caactacctg
      301 cctcaattaa cctatctcgc tgaagcaaag gacctaaatt tggaattagc cagtcatttc
      361 cagttaagac tggaagaaac ttcagagcgg aaatggagcg gaggggaaga acgaaagatc
      421 gagttgatac gtcttctttc ttcttatgaa caagggatgc ttctagacga gccaacaacc
      481 catctagata gaaaaagtat tgatcgactg attgaagagc ttcgttatta ttatggtacg
      541 ctggtttttg ttagtcatga tcgctatttt ctagatgagc tagcatcgaa aatctgggaa
      601 gtaaaagacg gagaaatccg agagttttcg gggaattata gtgcctatct cactcaaaag
      661 gaattggaga aaaagactca gctacgagaa gcagagtcga tcatgaaaga gaaaaaacga
      721 ttggaaaaat cgatccaaga aaagaaaaaa caagcggaaa agttggaaaa agtgtccagt
      781 aaaaagaaaa agcaacaaat cagactggat cggttgtctt cctctaaaca aaaagacagt
      841 gtacaaaaag ccatccaaaa gaatgcgaaa acattagaga gaagactcca aaaaatagga
      901 gaaacaacca agccgcagca gatgaaacaa atccgttttc cagtaccaaa atctcttgaa
      961 ctccccagcc gctatcccat catgggacaa aatgtccaat tggaaagaag tgggagaaca
     1021 ttactggtaa atggtgattt tcagttttct ttaggtaaaa aaatcgcgat tgtcggtgaa
     1081 aatggttcag gtaagacaac cttattagaa catatccgca aacaaggaga aggaatcctt
     1141 ctctctccga aagtaagctt tcaagtatat cagcaaaagg gttatcaaat gacatctgaa
     1201 gaatccatca ttcgttttgt catgagacaa acagagtttt cagaatcgct tgtccgtagt
     1261 ttgctgaatc acttagggtt tgctcaggaa actttgacga aaccgttatg tacattaagt
     1321 gggggagaag cgacccgtct gacgattgct ttgcttttta ctaagccaag taatgtgttg
     1381 ctgttagatg aaccgactaa ttttattgat atggcaacga tcgaagcttt agagaagcta
     1441 atgcaaatat atccgggaac gattttgttt acttctcatg attcttactt tgtcgagcgt
     1501 acggctgatg aagtttatga aataaaaggg cagaaaataa aaaaagtact tacgagaaat
     1561 ttttaaatgt gctacgatga ttttcgtgac aaatttaaag gagtggtaca gattgcctac
     1621 aaataaaaaa gaaggaataa tttttacaac aatgatgtgt tttttgatgg tcttggggat
     1681 gagcgcgtat aatttatggc tgcatcaaga tcttcattta tcaagtttgt tgattggtct
     1741 agtgcctgga ttcgtggtgg ctttcatttt ggatgtcttc atcatcggtg tgctggcgaa
     1801 aaaaattgcc tttaaactac cagtaaatcc agaaagcaag gtccaaatga tcttaactat
     1861 ttcatgcttg atggttttag gaatggtgac atgtatgtct ttattcggtg tgatcatgga
     1921 acaaggaata cctgatcaat tattctctgc ttatatgaat gcttggaaaa tgaatgtgat
     1981 tgccgcttta ccgttgcagt tgatcatcgt tggcccgctt tcaagaagtg tgctaaaagc
     2041 aattcaaagt aatgaatgat ggaacactgt tatttgaaaa aaggccttgg catgcttttt
     2101 gtcatagtct cttgaatgca gttctagaaa aatagtgatc cgaacgatag gttttggcct
     2161 gtcgttcgga tcgctttttc ttttccaact gtattgctct tttattgaag ataatttgct
     2221 cttcaataga tatttctaga aaggttctgt ttatccctta ctcaaaaaac tgctgcattt
     2281 gttctcttac ttgctgacac cattggagtt cctgctgaca aagcataatt ctgcgctgga
     2341 tcacaacata acgtccaaat tcttgtttct tgtctagcga gaggtcattc aatttttttt
     2401 gatttttttg catgattttg ttcagctgtt gttctctagt gaatagcagt ccagtagctg
     2461 ttgttttatc gagcgtcgaa aaagcataga tttttgctag aaattcatct ttttgcgtag
     2521 gagcgggagt ttcctctttg atccattcct ctacaagagc acgtccttca tcagtcaagt
     2581 ggtagatctt tttttgagag tgctcgtctt tgtccaaaac ggtcacataa ccctgctggt
     2641 ctaacttggc aagcgttgta tagatctgac tgtgatgggc ttcccaaaaa attgtgatca
     2701 gctgcttcaa ttcataccca gaacgtggtt tggataaaag cacgcataat aatatatagg
     2761 ctaaagtatt cataaaaatc ctacctctcg tcttattttc tttattatat cacagtaaag
     2821 tttccacgtt ttacatgtgc atattgacat gtaaaataaa tcatgtatga tgctaataga
     2881 gagaaaggga tggaaatatt ggagaatcaa atagatatac aagaacaaac aaatgaagaa
     2941 acaaagggga aaagttcagc gctagttgta gcagctacct gtatgatcgc ttttatggga
     3001 gtgggtctag tagacccaat cttaaaaacg attgctgcac agttaaacgc tacgccagct
     3061 gaaacgaccc tgttatttac tagttacatg ttagtcactg gtgtggtgat gctatttaca
     3121 ggtttcttat ctagtcggat cggtttgaaa aaaacattga tgacaggatt gtttatcatc
     3181 gttgtattcg ctggtcttgg cggattatct gggaatatcg gaatgctgat tgggttacgt
     3241 gccggctggg gtgtcgggaa tgctttattt gtctctactg ctttagcaac gatcgtcagt
     3301 attttagttg gtaacacaga aaaagcaatc atgatgtatg aagcagcact tggctgcgga
     3361 atggctgttg gccctctagt cggtggggtc ttgggcagtg gttcatggcg ttatccattt
     3421 tttggggtag catttttgat gttttgcggt tttatagccc tagcggtact tttacctgaa
     3481 actgagaaac caaagagaaa agtaggcctt tgggaaggtg tcaaagcgtt aagcaatcat
     3541 aaacttcgtt cagttggact aatcgcattg ttatataatt tcggattctt tactttactt
     3601 gcgtatgcgc catttttatt gattggctat tctgaattag aagtcggttt tgtatttttt
     3661 ggctggggtg tgcttctggc tattgcttcg attttcgttg caccggctct tgagcggaag
     3721 tttacgactt atcggacaat tttagcagca tttgtcttat tcattatttg tttgctgtta
     3781 ttaggagctg gaacttctat gccagtcctt gtaccgatca gcattgtgtt agcaggattt
     3841 ttccaaggaa ttatcaatac actgttgacg acgattgcta tggaaatacc aggtctgcaa
     3901 cgaaacgttg cttcgtccag ctatagtttt gtccgctttt tcggtggagc tttagcacca
     3961 tttattgcag gtgtgattgg tgaacatatt ggtgaaagct atgcgtttta ttttgcaggt
     4021 gccattgttc ttatcagtgt atgttgtatc atttatcacc gtaattattt tgctacaaat
     4081 gaagaagaat aagaaatcag aggttgtgaa aaagctgtcc tgcatcaagt cataagaa
//
LOCUS       DAZKBH010000321         3638 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.172, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000321 DAZKBH010000000
VERSION     DAZKBH010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.172"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1030
                     /gene="vanS"
                     /locus_tag="ACG8OO_003050"
     CDS             <1..1030
                     /gene="vanS"
                     /locus_tag="ACG8OO_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFP9046847.1"
                     /translation="LFDSRFKTLQQNHKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVI
                     SILILCRVMLSKFAKYFDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKR
                     EQDAKLAEQRKNDVVMYLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKA
                     YRLEQLIDEFFEITRYNLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPE
                     DLTVSGDPDKLARVFNNILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKL
                     AAIFEKFYRLDNARSSDTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMP
                     DLVDKRRS"
     gene            1245..2213
                     /locus_tag="ACG8OO_003051"
     CDS             1245..2213
                     /locus_tag="ACG8OO_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9046848.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            2206..3237
                     /gene="vanA"
                     /locus_tag="ACG8OO_003052"
     CDS             2206..3237
                     /gene="vanA"
                     /locus_tag="ACG8OO_003052"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9046849.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            3243..3608
                     /locus_tag="ACG8OO_003053"
                     /pseudo
     CDS             3243..3608
                     /locus_tag="ACG8OO_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1104 a          703 c          866 g          965 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccac aaatatgact taaatcacct
       61 ggacgcgatg aaattatatc aatattccat acggaacaat atagatatct ttatttatgt
      121 ggcgattgtc attagtattc ttattctatg tcgcgtcatg ctttcaaaat tcgcaaaata
      181 ctttgacgag ataaataccg gcattgatgt acttattcag aacgaagata aacaaattga
      241 gctttctgcg gaaatggatg ttatggaaca aaagctcaac acattaaaac ggactctgga
      301 aaagcgagag caggatgcaa agctggccga acaaagaaaa aatgacgttg ttatgtactt
      361 ggcgcacgat attaaaacgc cccttacatc cattatcggt tatttgagcc tgcttgacga
      421 ggctccagac atgccggtag atcaaaaggc aaagtatgtg catatcacgt tggacaaagc
      481 gtatcgactc gaacagctaa tcgacgagtt ttttgagatt acacggtata acctacaaac
      541 gataacgcta acaaaaacgc acatagacct atactatatg ctggtgcaga tgaccgatga
      601 attttatcct cagctttccg cacatggaaa acaggcggtt attcacgccc ccgaggatct
      661 gaccgtgtcc ggcgaccctg ataaactcgc gagagtcttt aacaacattt tgaaaaacgc
      721 cgctgcatac agtgaggata acagcatcat tgacattacc gcgggcctct ccggggatgt
      781 ggtgtcaatc gaattcaaga acactggaag catcccaaaa gataagctag ctgccatatt
      841 tgaaaagttc tataggctgg acaatgctcg ttcttccgat acgggtggcg cgggacttgg
      901 attggcgatt gcaaaagaaa ttattgttca gcatggaggg cagatttacg cggaaagcaa
      961 tgataactat acgacgttta gggtagagct tccagcgatg ccagacttgg ttgataaaag
     1021 gaggtcctaa gagatgtata taatttttta ggaaaatctc aaggttatct ttactttttc
     1081 ttaggaaatt aacaatttaa tattaagaaa cggctcgttc ttacacggta gacttaatac
     1141 cgtaagaacg agccgttttc gttcttcaga gaaagatttg acaagattac cattggcatc
     1201 cccgttttat ttggtgcctt tcacagaaag ggttggtctt aattatgaat aacatcggca
     1261 ttactgttta tggatgtgag caggatgagg cagatgcatt ccatgctctt tcgcctcgct
     1321 ttggcgttat ggcaacgata attaacgcca acgtgtcgga atccaacgcc aaatccgcgc
     1381 ctttcaatca atgtatcagt gtgggacata aatcagagat ttccgcctct attcttcttg
     1441 cgctgaagag agccggtgtg aaatatattt ctacccgaag catcggctgc aatcatatag
     1501 atacaactgc tgctaagaga atgggcatca ctgtcgacaa tgtggcgtac tcgccggata
     1561 gcgttgccga ttatactatg atgctaattc ttatggcagt acgcaacgta aaatcgattg
     1621 tgcgctctgt ggaaaaacat gatttcaggt tggacagcga ccgtggcaag gtactcagcg
     1681 acatgacagt tggtgtggtg ggaacgggcc agataggcaa agcggttatt gagcggctgc
     1741 gaggatttgg atgtaaagtg ttggcttata gtcgcagccg aagtatagag gtaaactatg
     1801 taccgtttga tgagttgctg caaaatagcg atatcgttac gcttcatgtg ccgctcaata
     1861 cggatacgca ctatattatc agccacgaac aaatacagag aatgaagcaa ggagcatttc
     1921 ttatcaatac tgggcgcggt ccacttgtag atacctatga gttggttaaa gcattagaaa
     1981 acgggaaact gggcggtgcc gcattggatg tattggaagg agaggaagag tttttctact
     2041 ctgattgcac ccaaaaacca attgataatc aatttttact taaacttcaa agaatgccta
     2101 acgtgataat cacaccgcat acggcctatt ataccgagca agcgttgcgt gataccgttg
     2161 aaaaaaccat taaaaactgt ttggattttg aaaggagaca ggagcatgaa tagaataaaa
     2221 gttgcaatac tgtttggggg ttgctcagag gagcatgacg tatcggtaaa atctgcaata
     2281 gagatagccg ctaacattaa taaagaaaaa tacgagccgt tatacattgg aattacgaaa
     2341 tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat gggaaaacga caattgctat
     2401 tcagctgtac tctcgccgga taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa
     2461 tatgaaatca accatgttga tgtagcattt tcagctttgc atggcaagtc aggtgaagat
     2521 ggatccatac aaggtctgtt tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa
     2581 agctcagcaa tttgtatgga caaatcgttg acatacatcg ttgcgaaaaa tgctgggata
     2641 gctactcccg ccttttgggt tattaataaa gatgataggc cggtggcagc tacgtttacc
     2701 tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat
     2761 agcgcggacg aattggacta cgcaattgaa tcggcaagac aatatgacag caaaatctta
     2821 attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg
     2881 ttagttgttg gcgaggtgga ccaaatcagg ctgcagtacg gaatctttcg tattcatcag
     2941 gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa ccgttcccgc agacctttca
     3001 gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt
     3061 agaggtctag cccgtgtgga tatgttttta caagataacg gccgcattgt actgaacgaa
     3121 gtcaatactc tgcccggttt cacgtcatac agtcgttatc cccgtatgat ggccgctgca
     3181 ggtattgcac ttcccgaact gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa
     3241 gcatggaaat aggatttact tttttagatg aaatagtaca cggtgttcgt tgggacgcta
     3301 aatatgccac ttgggataat ttcaccggaa aaccggttga cggttatgaa gtaaatcgca
     3361 ttgtagggac atacgagttg gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc
     3421 aagggtacgg attgcttcta tgggacggtt accgtcctaa gcgtgctgta aactgtttta
     3481 tgcaatgggc tgcacagccg gaaaataacc tgacaaagga aagttattat cccaatattg
     3541 accgaactga gatgatttca aaaggatacg tggcttcaaa atcaagccat agccgcggcg
     3601 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAZKBH010000322         2960 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.185, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000322 DAZKBH010000000
VERSION     DAZKBH010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.185"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1052
                     /locus_tag="ACG8OO_003054"
     CDS             <1..1052
                     /locus_tag="ACG8OO_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9046850.1"
                     /translation="IKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPR
                     AKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDF
                     FLKYYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRG
                     ELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSR
                     YAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYC
                     RLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNS
                     RVKERLEQIIGSLTG"
     gene            1059..1436
                     /locus_tag="ACG8OO_003055"
     CDS             1059..1436
                     /locus_tag="ACG8OO_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFP9046851.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1590..2372
                     /locus_tag="ACG8OO_003056"
     CDS             1590..2372
                     /locus_tag="ACG8OO_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFP9046852.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2498..>2960)
                     /locus_tag="ACG8OO_003057"
     CDS             complement(2498..>2960)
                     /locus_tag="ACG8OO_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HFP9046853.1"
                     /translation="PKHINYKIYGNIPYNISTDIVKRITFESQAKYSYLIVEKGFAKR
                     LQNLQRALGLLLMVEMDIKMLKKVPPLYFHPKPSVDSVLIVLERHQPLISKKDYKKYR
                     SFVYKWVNREYRVLFTKNQFRQALKHANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT         1076 a          431 c          579 g          874 t
ORIGIN      
        1 gaataaaacc tagtacaata actgggagga tctctatatt ggaaggacta tttagtaccc
       61 ttcttaggct agaatgggat gatgttcctt ccaaaatatt aatttattct gaggactatc
      121 cgaaaatacc aagagcaaaa ccacgcttta tagatgaatt cgtcctagag caattgaaca
      181 gtcatcttga taaattaccc gaatatatag ctacgatgac tatgattgtt caagaatgtg
      241 gaatgaggat aagtgaattg tgcaccttga aaaaaggctg tctattagag gacaaagatg
      301 gagatttctt tttaaagtat tatcaatgga aaatgaaaaa ggagcatata gttccaatat
      361 ctaaagaggt agctttactt attaaagttc gggaagataa agtttcagag gaatttccag
      421 atagtgaata cctctttcca agaaaagatg gatcgccatt aaaacaagaa acatttagag
      481 gtgagttaaa taaattagct tatgagcaaa atatagtgga taaatcaggt gagatttata
      541 gattccatgc ccatgccttt cgccatacag taggaacaag aatgattaac aacgggatgc
      601 cccagcatat tgtgcagaaa tttttggggc atgaaagccc agaaatgaca agcagatacg
      661 ctcatatctt tgatgaaact ctaaaaaatg aatttactaa atttcaggaa aaactggtta
      721 ccaataatgg agatgtgctt gatctagatg aagataatga agtcgatgat gtagagcttc
      781 aatggttcaa gaaaaatata aatgcacaag tgcttccaaa tggttattgt agattgccag
      841 tagtagcagg tggttgtcca catgcgaatg catgcttaga ttgcactcac ttctgtacca
      901 gtaagcaatt cttaccacag cacgaagaac agttagagcg tacagaagag ttattagcca
      961 tagctaagga taaacaatgg caaagacaag tagagactaa tagccgtgtt aaagagcgtt
     1021 tagaacaaat cattggaagt ttgacggggt aattatcaat ggataaacaa gttagaaata
     1081 caacagaaat tgtacgtttg gcgaagcaga aatcaaaaaa gacaagggaa aaagtagaca
     1141 aagcgatttc taaattttcg attgaaggta aagttattaa ttttaattca atagcaaagg
     1201 aagctaatgt ttctaaatca tggctttata aggaacacga tattaggcaa agaatcgaat
     1261 cccttcgtga gcgtcaaata acagcaaatg tagtctcaaa acccaagaaa agttctcgtt
     1321 cggaggaaat ccttattaaa accttaaaaa gaagagtaat ggaattagaa aaagaaaata
     1381 aaaaattaca gaaccaaatt caaaaattat atggagatct gtataataaa gaataattat
     1441 taatctgtag acaaattgtg aaaggatgta cttaaacgct aacggtcagc tttattgaac
     1501 agtcatttag gtatatgtcc aatctagggt aagtaaattg agtatcaata taaactttat
     1561 atgaacataa tcagttacga ggtgaaatca tgagcaattt gattaacgga aaaataccaa
     1621 atcaagcgat tcaaacatta aaaatcgtaa aagatttatt tggaagttca atagttggag
     1681 tatatctatt tggttcagca gtaaatggtg gtttacgcat taacagcgat gtagatgttc
     1741 tagtcgtcgt gaatcatagt ttacctcaat taactcgaaa aaaactaaca gaaagactaa
     1801 tgactatatc aggaaagatt ggaaatacgg attctgttag accacttgaa gttacggtta
     1861 taaataggag tgaagttgtc ccttggcaat atcctccaaa aagagaattt atatacggtg
     1921 agtggctcag gggtgaattt gagaatggac aaattcagga accaagctat gatcctgatt
     1981 tggctattgt tttagcacaa gcaagaaaga atagtatttc tctatttggt cctgattctt
     2041 caagtatact tgtctccgta cctttgacag atattcgaag agcaattaag gattctttgc
     2101 cagaactaat tgaggggata aaaggtgatg agcgtaatgt aattttaacc ctagctcgaa
     2161 tgtggcaaac agtgactact ggtgaaatta cctcgaaaga tgtcgctgca gaatgggcta
     2221 tacctctttt acctaaagag catgtaactt tactggatat agctagaaaa ggctatcggg
     2281 gagagtgtga tgataagtgg gaaggactat attcaaaggt gaaagcactc gttaagtata
     2341 tgaaaaattc tatagaaact tctctcaatt aggctaattt tattgcaata acaggtgctt
     2401 acttttaaaa ctactgattt attgataaat attgaacaat ttttgggaag aataaagcgt
     2461 cctcttgtga aattagagaa cgctttatta ctttaattta gtgaaacaat ttgtaactat
     2521 tgaaaataga aagaaattgt tccttcgata gtttattaat attagtgaca tttgcatgct
     2581 tcaaagcctg tcggaattgg tttttagtga aaagaacacg atattcacgg tttacccact
     2641 tataaacaaa agatcgatac tttttgtagt ccttctttga aatcaatggt tgatgtcgtt
     2701 caagaacaat caatacagag tctacacttg gcttaggatg aaaatatagt ggtggtactt
     2761 ttttgagcat ttttatatcc atctccacca ttaatagtaa acccaaagct cgttgcagat
     2821 tttgcaatct tttcgcaaat cccttctcaa cgataagata gctatattta gcctgacttt
     2881 caaaggtaat tcttttgaca atatccgtac tgatgttata aggaatatta ccatatatct
     2941 tatagtttat atgttttggg
//
LOCUS       DAZKBH010000323         2439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.196, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000323 DAZKBH010000000
VERSION     DAZKBH010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.196"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..1171)
                     /locus_tag="ACG8OO_003058"
     CDS             complement(68..1171)
                     /locus_tag="ACG8OO_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9046854.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1724..2272
                     /gene="aac(6')"
                     /locus_tag="ACG8OO_003059"
     CDS             1724..2272
                     /gene="aac(6')"
                     /locus_tag="ACG8OO_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9046855.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          772 a          432 c          478 g          757 t
ORIGIN      
        1 ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag
       61 caattattta actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt
      121 ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa
      181 tatcatttgt tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat
      241 tcatgtcggt taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa
      301 acaaatcgaa ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc
      361 cgtgaaggta tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca
      421 tagcagaatt atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat
      481 ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt
      541 gcgtaccata agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat
      601 ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag
      661 catcgccaat acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa
      721 tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt
      781 tatagatgac acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga
      841 agactggttt agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac
      901 cgactgcaaa caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat
      961 ttccataagt tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca
     1021 aagctacttt tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta
     1081 cttttgtccc atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg
     1141 tataatttgg taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg
     1201 ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc
     1261 taagacagga taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc
     1321 ctaaaaacga acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta
     1381 gtatttctac cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat
     1441 tattatatga aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga
     1501 taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct
     1561 ttcctatttt gaataaagtg tttctgtata atttttttat tttataagta agataaataa
     1621 tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt
     1681 tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt
     1741 tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc
     1801 ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat
     1861 cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata
     1921 cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca
     1981 aataggtact cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac
     2041 gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta
     2101 tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt
     2161 ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa
     2221 accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga
     2281 ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt
     2341 gaatttttgt gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtacca
     2401 gaggttgtga aaaagtcgct tagtttcgag tagtaagaa
//
LOCUS       DAZKBH010000324         2366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.199, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000324 DAZKBH010000000
VERSION     DAZKBH010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.199"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..530)
                     /locus_tag="ACG8OO_003060"
     CDS             complement(<1..530)
                     /locus_tag="ACG8OO_003060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9046856.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTS"
     gene            922..981
                     /locus_tag="ACG8OO_003061"
     CDS             922..981
                     /locus_tag="ACG8OO_003061"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFP9046857.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            1039..1770
                     /gene="erm(A)"
                     /locus_tag="ACG8OO_003062"
     CDS             1039..1770
                     /gene="erm(A)"
                     /locus_tag="ACG8OO_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFP9046858.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(1896..>2366)
                     /locus_tag="ACG8OO_003063"
     CDS             complement(1896..>2366)
                     /locus_tag="ACG8OO_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HFP9046859.1"
                     /translation="REFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGP
                     DSSSILVSVPLTDIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVA
                     AEWAIPLLPKEHVTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          816 a          432 c          352 g          766 t
ORIGIN      
        1 ttagatgtgt tgtgattata aattctaatc gcatttttta ttccaaattt gaaacaatgc
       61 ggtaaaaact cttggattgc ccctaattta taaacatagg taggcggtgt ctcgacttca
      121 gatacattat caagaataac tattcttcct tcctcattca acagttcctt catttgctgt
      181 attacgctgg ctatatcatc caaatgatga aaggttgttc ggcttacaat aaaatcaaac
      241 ttctcattaa aattaagttg ttctgcattc atattcagat agaccgtatt tgttagttga
      301 cgtttagatt tggcaagatc gagcatttga ttagaaatat caatccctac cacttcatca
      361 taataacttg ctaatttctc cactaacaaa cccgagccac atccgatatc taatgctctg
      421 cctttctttg gagacatatt agacacaaag aatgaataat cattcaaaag ctcattcacg
      481 aaatcgtaat cttctgcaac cttatcaaac tgtgattcta ttgtattcaa aaagatcccc
      541 cattcctact ttatcgacat tctttcatta cttaccactt tagatgtttt ttcgttgggg
      601 ataaaacttc cctttagaca attttatcca aagacaatac aacagtgcaa ctttattaaa
      661 gtcactgtcc tttatcgcag cctttacttt ttagtaaaga cagtggcttc tcttatcaag
      721 tttcaaaaca tattattttg aagaaaacgt ccatctgaag tgtcaagtgc aaaattacat
      781 ataaaggttt attctaaaat gaaaagatga tacaatcata ttcagttaca taaggaggtt
      841 tcaattatgt gcaccagtat cgcagtagta gaaattactt tatctcattc ataatgaaaa
      901 aaatggaaag gagataaaag tatgggtact ttttctatat ttgttattaa taaagttcgt
      961 tatcaaccaa atcaaaatta attggttata atgaacgctt aatgtcagtt cattataacc
     1021 agtaaggaga aggttataat gaaccagaaa aaccctaaag acacgcaaaa ttttattact
     1081 tctaaaaagc atgtaaaaga aatattgaat cacacgaata tcagtaaaca agacaacgta
     1141 atagaaatcg gatcaggaaa aggacatttt accaaagagc tagtcaaaat gagtcgatca
     1201 gttactgcta tagaaattga tggaggctta tgtcaagtga ctaaagaagc ggtaaacccc
     1261 tctgagaata taaaagtgat tcaaacggat attctaaaat tttccttccc aaaacatata
     1321 aactataaga tatatggtaa tattccttat aacatcagta cggatattgt caaaagaatt
     1381 acctttgaaa gtcaggctaa atatagctat cttatcgttg agaagggatt tgcgaaaaga
     1441 ttgcaaaatc tgcaacgagc tttgggttta ctattaatgg tggagatgga tataaaaatg
     1501 ctcaaaaaag taccaccact atattttcat cctaagccaa gtgtagactc tgtattgatt
     1561 gttcttgaac gacatcaacc attgatttca aagaaggact acaaaaagta tcgatctttt
     1621 gtttataagt gggtaaaccg tgaatatcgt gttcttttca ctaaaaacca attccgacag
     1681 gctttgaagc atgcaaatgt cactaatatt aataaactat cgaaggaaca atttctttct
     1741 attttcaata gttacaaatt gtttcactaa attaaagtaa taaagcgttc tctaatttca
     1801 caagaggacg ctttattctt cccaaaaatt gttcaatatt tatcaataaa tcagtagttt
     1861 taaaagtaag cacctgttat tgcaataaaa ttagcctaat tgagagaagt ttctatagaa
     1921 tttttcatat acttaacgag tgctttcacc tttgaatata gtccttccca cttatcatca
     1981 cactctcccc gatagccttt tctagctata tccagtaaag ttacatgctc tttaggtaaa
     2041 agaggtatag cccattctgc agcgacatct ttcgaggtaa tttcaccagt agtcactgtt
     2101 tgccacattc gagctagggt taaaattaca ttacgctcat caccttttat cccctcaatt
     2161 agttctggca aagaatcctt aattgctctt cgaatatctg tcaaaggtac ggagacaagt
     2221 atacttgaag aatcaggacc aaatagagaa atactattct ttcttgcttg tgctaaaaca
     2281 atagccaaat caggatcata gcttggttcc tgaatttgtc cattctcaaa ttcacccctg
     2341 agccactcac cgtatataaa ttctct
//
LOCUS       DAZKBH010000325         2243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.205, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000325 DAZKBH010000000
VERSION     DAZKBH010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.205"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..847)
                     /locus_tag="ACG8OO_003064"
     CDS             complement(<1..847)
                     /locus_tag="ACG8OO_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046860.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYR"
     gene            940..1289
                     /locus_tag="ACG8OO_003065"
                     /pseudo
     CDS             940..1289
                     /locus_tag="ACG8OO_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1561..1851)
                     /locus_tag="ACG8OO_003066"
     CDS             complement(1561..1851)
                     /locus_tag="ACG8OO_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9046861.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(2059..>2243)
                     /locus_tag="ACG8OO_003067"
     CDS             complement(2059..>2243)
                     /locus_tag="ACG8OO_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9046862.1"
                     /translation="LTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVE
                     DTADKSQQIVEEHLRK"
BASE COUNT          732 a          407 c          309 g          795 t
ORIGIN      
        1 tcctatatgt tagaaggaaa attaagaaaa atagcaataa aaataagata ttcatgtcta
       61 aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa cttattaaga aagttgaaat
      121 ttttatattc aggaaaccat ttattaattt catgtgcagc acgtaatgca ttccctggag
      181 ttgtcaaaga gaaaattaat tctgctaagc ttattattga aaatactgat aacttataat
      241 tatatcgtcc attgacaatc aaatataaac taactatact tgttagaaca aaaaaacagg
      301 cgttgacttg ttcttgatta gttaaaaata tcaataaagg aatacttacc caatatattt
      361 ttctatttat ttcttctcct cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa
      421 atgctactgg ccactgatag ttcaaggtgg ttgctctcca accggtttcc cccattactg
      481 tcaatggtat taaacagtat atagagcaaa ccaataataa atttttaaca tttattttct
      541 caaaaacata cttgcaaagt aatactacaa ttcctaacat aatcgtagaa tttaataatt
      601 tccatagcat aaagtgttta gaaaagaaca ttaagaatag ttctataata attcttgaac
      661 tccatgtctt ataatgttct atcgaagcac ccaaatagcc aagttcatct aatctacttg
      721 cataaactac gtcatccgct ctcatagtcg gcatatacat attaaagagt aaaaagatac
      781 cccaaatccc tattagtaac aagatcagtt ctttgttatt cgttattttt ctattatgtt
      841 tgttcatttt tatactcctt cttatatttc ctaatagagt ataacatagc tttaagcatt
      901 acttaaagat aacttaaaag ttatctttta aatacttaat tgttaaatac cttatttgat
      961 gcaaaagaaa tctacgatag tcaacaaaaa aattttcatt atatccagaa aaatttttct
     1021 tagttaagga cctatggatt gcagtaatgc agaattcgtc gaataaactg ccaaagacat
     1081 acaatcatat tgcttttaaa attgacgaac aagatataga ttcgtttgtc tcaaaaatcc
     1141 aaatgttagg tttaactgtc gaaccaggac gttctcgtgt gaaaggcgag gcttcttcaa
     1201 tttactttta cgactatgat aatcacctct tcgagttaca taccggtact ctccaggaac
     1261 gtttaaaatc ctacaattcc actacctgag actagtaatg actagtctct ttttatggcc
     1321 tctgatcccc ccattgagcg aatactaata gggcttcgaa ttgatctgtt caatctttaa
     1381 gcaatatccc tcaaatgagt ttggaaagtt tagtgattca tcacaaatac ttaaatacat
     1441 gtagttatat atagattgag gttcttgtga gcgtatgctc gtgtgggttc aaatcccatc
     1501 gtctgcatcc atctttccaa aacaagcggc taggacattt ttgtctcagc cgcttgtttt
     1561 ctagtttatt ctttcatctt gtcggacctt ttttcttggg tatagacttt tctcatattc
     1621 taccacaatt ttgggtttcc ctaacacatg ataggatctc acaacaaatt gctttctatt
     1681 attataataa cctgtaacga ctaacttcat cccttcgtct acttcagcta ggaagttcaa
     1741 actatgggaa gcaatcaaac aattgacccc atttaaactg aaacggatca acggtctggc
     1801 actaacttgc aaagtacgga tcttatctac tgttccatat tttttctcca taaccttcac
     1861 ctcaatacca ttatacgaac atacattccc tttgtaaaga agaaactctg ttttatcttt
     1921 ttgacttttt gtctattacc tttcttaaaa tcatattggc acttcctgcg aagatgaagg
     1981 acatgtacta aaaaaaagag gcagcagtat agattactgt tgcttcttta ctaatttagg
     2041 tttacttgtt aagaaagatt atttacgcaa atgttcttca acaatttgct gagatttatc
     2101 agctgtatct tccacttcat cttttgcttt ttctaacttt tgaatatatt gtttcaagct
     2161 tgagttttca gcagttgctt cgtctaattt cttttgtgta tctgttaact gagtttctac
     2221 atttgttttt tcttgtgtca gtg
//
LOCUS       DAZKBH010000326         2059 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.212, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000326 DAZKBH010000000
VERSION     DAZKBH010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.212"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..1026)
                     /locus_tag="ACG8OO_003068"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="ACG8OO_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACG8OO_003069"
     CDS             1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACG8OO_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP9046863.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..2021
                     /locus_tag="ACG8OO_003070"
                     /pseudo
     CDS             1851..2021
                     /locus_tag="ACG8OO_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
BASE COUNT          627 a          400 c          407 g          625 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tggttctgtt gcaaagtttt
     2041 aaataaagaa taaaatccc
//
LOCUS       DAZKBH010000327         1781 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.224, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000327 DAZKBH010000000
VERSION     DAZKBH010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.224"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..416
                     /locus_tag="ACG8OO_003071"
     CDS             24..416
                     /locus_tag="ACG8OO_003071"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9046864.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8OO_003072"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8OO_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFP9046865.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DAZKBH010000328         1504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.233, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000328 DAZKBH010000000
VERSION     DAZKBH010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.233"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACG8OO_003073"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACG8OO_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACG8OO_003074"
     CDS             394..477
                     /locus_tag="ACG8OO_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFP9046866.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG8OO_003075"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG8OO_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP9046867.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAZKBH010000329         1382 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.242, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000329 DAZKBH010000000
VERSION     DAZKBH010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.242"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1311
                     /locus_tag="ACG8OO_003076"
     CDS             <1..1311
                     /locus_tag="ACG8OO_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFP9046868.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DAZKBH010000330         1350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.245, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000330 DAZKBH010000000
VERSION     DAZKBH010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.245"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..298
                     /locus_tag="ACG8OO_003077"
                     /pseudo
     CDS             47..298
                     /locus_tag="ACG8OO_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ACG8OO_003078"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ACG8OO_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFP9046869.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAZKBH010000331         1194 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4462
            SAMEA115707139-rid22576153.guided.258, whole genome shotgun
            sequence.
ACCESSION   DAZKBH010000331 DAZKBH010000000
VERSION     DAZKBH010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707139
            Sequence Read Archive: ERR13196870
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287896.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:01:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,079
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 34 of 93
            Pseudo Genes (incomplete)         :: 49 of 93
            Pseudo Genes (internal stop)      :: 25 of 93
            Pseudo Genes (multiple problems)  :: 12 of 93
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707139-rid22576153.guided.258"
                     /isolate="VRE4462"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="ACG8OO_003079"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="ACG8OO_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFP9046870.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
